CN105601605A - Methanol hydrochloric acid extraction method of anthocyanins in aged purple chrysanthemum flower petals - Google Patents
Methanol hydrochloric acid extraction method of anthocyanins in aged purple chrysanthemum flower petals Download PDFInfo
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- CN105601605A CN105601605A CN201610099570.XA CN201610099570A CN105601605A CN 105601605 A CN105601605 A CN 105601605A CN 201610099570 A CN201610099570 A CN 201610099570A CN 105601605 A CN105601605 A CN 105601605A
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- hydrochloric acid
- aged
- purple chrysanthemum
- methanol
- purple
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
Abstract
The invention discloses a methanol hydrochloric acid extraction method of anthocyanins in aged purple chrysanthemum petals, and belongs to the technical field of extraction methods of plant anthocyanins. According to the technical scheme, the method comprises the steps that petals of fresh purple chrysanthemum flowers harvested in autumn are naturally dried at an indoor ventilation place and put at a dark and dry place for aging for three months, aged purple chrysanthemum flower petals and extract liquid composed of methanol and a hydrochloric acid solution are mixed to be uniform, and extraction is conducted for 24 h at 50 DEG C, wherein the molar concentration of the hydrochloric acid solution is 0.1 mol/L, the volume ratio of methanol to the hydrochloric acid solution is 1 to 3, and 1 g of aged purple chrysanthemum petals correspond to 70 mL of the extract liquid. According to the methanol hydrochloric acid extraction method of the anthocyanins in the aged purple chrysanthemum petals, methanol and the hydrochloric acid solution are adopted to prepare the extract liquid, the proportion of methanol in the extract liquid is lower, the usage amount of the extract liquid is increased, the temperature is increased compared with the original scheme, and the extraction effect of the anthocyanins in the aged purple chrysanthemum petals is obviously improved.
Description
Technical field
The invention belongs to the leach extraction method technical field of Anthocyanin, be specifically related to one and display rear purple Chrysanthemum PetalThe methanolic hydrochloric acid leach extraction method of anthocyanidin.
Background technology
Anthocyanidin (anthocyanins) also claim anthocyanin, is a kind of natural food colour, can remove in human body superfluousFree radical, can cross over blood-brain barrier and directly protect brain central nervous system, has radioresistance, uvioresistant, anti-sudden change and anti-Tumour, angiocardiopathy preventing, the functions such as alleviating asthenopia. Therefore,, about extract the research of anthocyanidin from plant, be day by day subject toTo people's concern.
Chrysanthemum (DendranthemamorifoliumRamat.), the herbaceous plant of genus composite family Chrysanthemum perennial root, petal canBe used for viewing and admiring, or be directly used in tea use, medicinal, edible, also can be used as important composition and add for the production of chrysanthemum crystals, chrysanthemum wineWork. In purple Chrysanthemum Petal, be rich in anthocyanidin, conduct a research for its extraction process, for realizing prolonging of agricultural product industrial chainStretch, increase flower-grower's income and have great importance. The fresh petal of this seminar early-stage Study purple chrysanthemum used is after gatheringNaturally dry under condition, extract anthocyanidin wherein without displaying direct employing extraction, Preliminary screening has gone out best techniqueCondition, finds taking the mixed solution of volume fraction 80% ethanol and molar concentration 0.1mol/L hydrochloric acid as extraction solvent ethanol and saltThe volume ratio of acid is 1:2, and solid-liquid ratio is 1:50(1g:50mL), at room temperature lixiviate 24h, lixiviate 2 times, anthocyanidin extraction effectBest. But in follow-up study, find, purple Chrysanthemum Petal is placed after 3 months (displaying rear chrysanthemum), with reference to aforementioned process conditionsWhile extracting anthocyanidin, there is the phenomenon of extracting effect variation.
Summary of the invention
The technical problem that the present invention solves has been to provide a kind of methoxide acidleach of displaying rear purple Chrysanthemum Petal anthocyanidinExtracting method, the method has effectively been improved the extraction effect of displaying rear purple Chrysanthemum Petal anthocyanidin.
The present invention adopts following technical scheme for solving the problems of the technologies described above, and one is displayed rear purple Chrysanthemum Petal anthocyanidinMethanolic hydrochloric acid leach extraction method, it is characterized in that concrete steps are: the fresh purple Chrysanthemum Petal of gathering autumn is at room ventilationPlace's nature dries, then displays 3 months in the dry place of dark, then by the purple Chrysanthemum Petal after displaying and methyl alcohol and hydrochloric acid solutionAfter the leaching liquor of composition mixes, in 50 DEG C of lixiviate 24h, wherein the molar concentration of hydrochloric acid solution is 0.1mol/L, methyl alcohol and saltThe volume ratio of acid solution is 1:3, and the volume of the corresponding leaching liquor of purple Chrysanthemum Petal after 1g displays is 70mL.
The present invention adopts methyl alcohol and hydrochloric acid solution preparation leaching liquor, and the ratio of methyl alcohol in leaching liquor is lower, and leaching liquor makesConsumption increases, and temperature improves than original scheme, displays rear purple Chrysanthemum Petal anthocyanidin extracting effect and obviously improves. For solving purpleIn look Chrysanthemum Petal anthocyanidin industrialization leaching process, because raw material are displayed and strengthened extracting effect variation because extracting difficulty afterwardsPractical problem provides effective means.
Detailed description of the invention
By the following examples foregoing of the present invention is described in further details, but this should be interpreted as to thisThe scope of inventing above-mentioned theme only limits to following embodiment, and all technology realizing based on foregoing of the present invention all belong to thisBright scope.
Embodiment 1
The present embodiment is that purple Dendranthema morifolium Varieties ' whole spice path ' is tested, and concrete control method is: that gathers autumn is freshPurple Chrysanthemum Petal dries naturally at room ventilation place, then displays 3 months in the dry place of dark, then by the purple chrysanthemum after displayingAfter the leaching liquor that flower petal and methyl alcohol and hydrochloric acid solution form mixes in 50 DEG C of lixiviate 24h, wherein hydrochloric acid solution moleConcentration is 0.1mol/L, and the volume ratio of methyl alcohol and hydrochloric acid solution is 1:3, the corresponding leaching liquor of purple Chrysanthemum Petal after 1g displaysVolume is 70mL.
Comparative example 1
This comparative example is that purple Dendranthema morifolium Varieties ' whole spice path ' is tested, and concrete control method is: that gathers autumn is freshPurple Chrysanthemum Petal dries naturally at room ventilation place, then purple Chrysanthemum Petal and ethanolic solution and hydrochloric acid solution is formedAfter leaching liquor mixes, in room temperature lixiviate 24h, wherein in ethanolic solution, the volume fraction of ethanol is 80%, the rubbing of hydrochloric acid solutionYour concentration is 0.1mol/L, and the volume ratio of ethanolic solution and hydrochloric acid solution is 1:2, and the purple Chrysanthemum Petal correspondence after 1g dries is soakedThe volume of extract is 50mL.
Comparative example 2
This comparative example is that purple Dendranthema morifolium Varieties ' whole spice path ' is tested, and concrete control method is: that gathers autumn is freshPurple Chrysanthemum Petal dries naturally at room ventilation place, then displays 3 months in the dry place of dark, then by the purple chrysanthemum after displayingAfter the leaching liquor that flower petal and ethanolic solution and hydrochloric acid solution form mixes in room temperature lixiviate 24h, wherein in ethanolic solutionThe volume fraction of ethanol is 80%, and the molar concentration of hydrochloric acid solution is 0.1mol/L, and the volume ratio of ethanolic solution and hydrochloric acid solution is1:2, the volume of the corresponding leaching liquor of purple Chrysanthemum Petal after 1g dries is 50mL.
' whole spice path ' petal anthocyanidin extraction effect comparison under the different extracting conditions of table 1
Note: upper and lower case letter represents respectively the significance of difference of same column data in 0.01 and 0.05 level.
More than experiment shows: adopt the extracting condition of this case, can effectively improve and display rear purple Chrysanthemum Petal anthocyanidinExtracting effect, thereby prove that the new technology of this lixiviate purple Chrysanthemum Petal anthocyanidin is conducive to solve industrial production reallyIn, because the problem of the anthocyanidin lixiviate difficulty causing deposited process and extend by raw material.
Above embodiment has described general principle of the present invention, principal character and advantage, and the technical staff of the industry shouldUnderstand, the present invention is not restricted to the described embodiments, and just illustrating of describing in above-described embodiment and description is of the present invention formerReason, is not departing under the scope of the principle of the invention, and the present invention also has various changes and modifications, and these changes and improvements all fall intoIn the scope of protection of the invention.
Claims (1)
1. display a methanolic hydrochloric acid leach extraction method for rear purple Chrysanthemum Petal anthocyanidin, it is characterized in that concrete steps are: the autumnThe fresh purple Chrysanthemum Petal of Ji Caishou dries naturally at room ventilation place, then displays 3 months in the dry place of dark, then by oldAfter the leaching liquor of the purple Chrysanthemum Petal after putting and methyl alcohol and hydrochloric acid solution composition mixes in 50 DEG C of lixiviate 24h, wherein saltThe molar concentration of acid solution is 0.1mol/L, and the volume ratio of methyl alcohol and hydrochloric acid solution is 1:3, the purple Chrysanthemum Petal after 1g displaysThe volume of corresponding leaching liquor is 70mL.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610099570.XA CN105601605B (en) | 2016-02-24 | 2016-02-24 | A kind of methanolic hydrochloric acid leach extraction method for displaying rear purple Chrysanthemum Petal anthocyanidin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610099570.XA CN105601605B (en) | 2016-02-24 | 2016-02-24 | A kind of methanolic hydrochloric acid leach extraction method for displaying rear purple Chrysanthemum Petal anthocyanidin |
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| CN105601605A true CN105601605A (en) | 2016-05-25 |
| CN105601605B CN105601605B (en) | 2018-05-08 |
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| CN201610099570.XA Active CN105601605B (en) | 2016-02-24 | 2016-02-24 | A kind of methanolic hydrochloric acid leach extraction method for displaying rear purple Chrysanthemum Petal anthocyanidin |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103626733A (en) * | 2013-11-08 | 2014-03-12 | 天津大学 | Separation method of blueberry anthocyanin |
| CN105223049A (en) * | 2015-08-28 | 2016-01-06 | 河南科技大学 | A kind of extracting method of plant leaf blade anthocyanidin |
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2016
- 2016-02-24 CN CN201610099570.XA patent/CN105601605B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103626733A (en) * | 2013-11-08 | 2014-03-12 | 天津大学 | Separation method of blueberry anthocyanin |
| CN105223049A (en) * | 2015-08-28 | 2016-01-06 | 河南科技大学 | A kind of extracting method of plant leaf blade anthocyanidin |
Non-Patent Citations (2)
| Title |
|---|
| 刘海英等: "采后处理对紫色菊花花瓣花青素吸收光谱及抗氧化活性的影响", 《湖北农业科学》 * |
| 王少朋等: "菊花花青素的粗提及其对DPPH的清除作用", 《西北农业学报》 * |
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| CN105601605B (en) | 2018-05-08 |
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