CN105597370B - 分离基质 - Google Patents

分离基质 Download PDF

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Publication number
CN105597370B
CN105597370B CN201610085613.9A CN201610085613A CN105597370B CN 105597370 B CN105597370 B CN 105597370B CN 201610085613 A CN201610085613 A CN 201610085613A CN 105597370 B CN105597370 B CN 105597370B
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CN
China
Prior art keywords
isolation medium
ligand
base matrix
growing agent
matrix
Prior art date
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CN201610085613.9A
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English (en)
Chinese (zh)
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CN105597370A (zh
Inventor
A.阿克森
E.布雷坎
G.埃德格伦
G.马尔姆奎斯特
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Cytiva Bioprocess R&D AB
Global Life Sciences Solutions USA LLC
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GE Healthcare Bio Sciences AB
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3285—Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/08—Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/16—Organic material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/08—Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/16—Organic material
    • B01J39/18—Macromolecular compounds
    • B01J39/19—Macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/08—Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/12—Macromolecular compounds
    • B01J41/13—Macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Other Resins Obtained By Reactions Not Involving Carbon-To-Carbon Unsaturated Bonds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CN201610085613.9A 2009-10-12 2010-10-08 分离基质 Active CN105597370B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0950748 2009-10-12
SE0950748-4 2009-10-12
CN2010800469014A CN102574101A (zh) 2009-10-12 2010-10-08 分离基质

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN2010800469014A Division CN102574101A (zh) 2009-10-12 2010-10-08 分离基质

Publications (2)

Publication Number Publication Date
CN105597370A CN105597370A (zh) 2016-05-25
CN105597370B true CN105597370B (zh) 2019-03-05

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CN2010800469014A Pending CN102574101A (zh) 2009-10-12 2010-10-08 分离基质

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Country Status (6)

Country Link
US (1) US20120202976A1 (enExample)
EP (1) EP2488295A1 (enExample)
JP (1) JP5826180B2 (enExample)
CN (2) CN105597370B (enExample)
IN (1) IN2012DN02539A (enExample)
WO (1) WO2011046494A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140228539A1 (en) * 2011-10-21 2014-08-14 Tanvex Biologics Corp. Separation of acetylated proteins from unacetylated proteins
CH706332B1 (de) * 2012-03-28 2015-10-15 Zeochem Ag Dotierte Materialien für die Umkehrphasen-Chromatographie.
JP2015212620A (ja) * 2012-08-28 2015-11-26 信和化工株式会社 コアシェル粒子の製造方法
PL2970378T3 (pl) 2013-03-15 2021-12-06 Biogen Ma Inc. Chromatografia oddziaływań hydrofobowych białek w warunkach bez soli
RU2665442C2 (ru) * 2013-10-10 2018-08-29 ДжиИ Хелткер Биопроцесс АрЭндДи АБ Способ получения хроматографического материала
JP6634202B2 (ja) * 2014-08-22 2020-01-22 Jsr株式会社 担体、担体の製造方法、及び標的物の精製方法
DE102016004432A1 (de) 2016-04-12 2017-10-12 Sartorius Stedim Biotech Gmbh Multimodales Adsorptionsmedium mit multimodalen Liganden, Verfahren zu dessen Herstellung und dessen Verwendung
EP3783009A1 (en) * 2017-02-10 2021-02-24 Mitsubishi Chemical Corporation Separating agent for human insulin purification and human insulin purification method
CN106824307B (zh) * 2017-02-21 2019-12-10 博格隆(上海)生物技术有限公司 一种混合型阴离子交换介质及其制备方法
CN116174058A (zh) * 2023-02-17 2023-05-30 常州天地人和生物科技有限公司 一种磺酸基阳离子交换介质的制备方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6115684A (ja) * 1984-07-02 1986-01-23 Asahi Chem Ind Co Ltd 全多孔質のスペ−サ−分子付担体
DE3811042A1 (de) * 1988-03-31 1989-10-19 Merck Patent Gmbh Ionenaustauscher
SE9601368D0 (sv) * 1996-04-11 1996-04-11 Pharmacia Biotech Ab Process for the production of a porous cross-linked polysaccharide gel
SE9700383D0 (sv) * 1997-02-04 1997-02-04 Pharmacia Biotech Ab An adsorption/separation method and a medium for adsorption/separation
US6310199B1 (en) * 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
SE0004932D0 (sv) * 2000-12-31 2000-12-31 Apbiotech Ab A method for mixed mode adsorption and mixed mode adsorbents
AU2002235795A1 (en) * 2000-12-31 2002-07-16 Amersham Biosciences Ab A method for the manufacture of compositions containing low concentrations of salts
SE0302911D0 (sv) * 2003-10-31 2003-10-31 Amersham Biosciences Ab Novel separation matrix
EP2066418A4 (en) * 2006-09-29 2011-12-28 Ge Healthcare Bio Sciences Ab SEPARATION MATRIX FOR VIRAL PURIFICATION
CN101678318B (zh) * 2007-05-25 2013-09-25 默克专利股份公司 用于阳离子交换色谱法的接枝共聚物
EP2153877A1 (de) * 2008-07-30 2010-02-17 MERCK PATENT GmbH Mischpfropfpolymere für die Ionenaustauschchromatographie
JP5396933B2 (ja) * 2009-03-11 2014-01-22 東ソー株式会社 液体クロマトグラフィー用充填剤、及び生体高分子の分離精製方法

Also Published As

Publication number Publication date
EP2488295A1 (en) 2012-08-22
JP2013507237A (ja) 2013-03-04
US20120202976A1 (en) 2012-08-09
WO2011046494A1 (en) 2011-04-21
JP5826180B2 (ja) 2015-12-02
CN102574101A (zh) 2012-07-11
IN2012DN02539A (enExample) 2015-08-28
CN105597370A (zh) 2016-05-25

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