CN1055880A - Processs for comprehensive recvery of blood - Google Patents
Processs for comprehensive recvery of blood Download PDFInfo
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- CN1055880A CN1055880A CN91103626A CN91103626A CN1055880A CN 1055880 A CN1055880 A CN 1055880A CN 91103626 A CN91103626 A CN 91103626A CN 91103626 A CN91103626 A CN 91103626A CN 1055880 A CN1055880 A CN 1055880A
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Abstract
A kind of separation of blood and recovery method, Processs for comprehensive recvery of blood particularly is made of the isolation technics in integrated use modern times, comprising centrifuging, ultrafiltration membrance filter method, chromatography and electrophoresis method, according to the characteristics of this technology respectively, planned combining makes blood in the process of each relevant devices of flowing through, with contained different components, from coarse to fine, all first separation is come out, thereby reaches recovery, the purpose of utilizing or checking, analyze.The inventive method efficient height can all separate useful component in the operation recovery blood remaining, so remarkable economic efficiency and social benefit are arranged.
Description
The present invention relates to a kind of separation and recovery method, particularly Processs for comprehensive recvery of blood of blood.
Current, the blood recovery system that uses of various countries in the world, blood recovery system as U.S. Heamenetics company produces can only separate cell in the blood and blood plasma by centrifugal device from the blood that operation is run off, its result just preserves various cells in the blood get off, blood plasma is then further separated and processing, cause the useful composition in the blood plasma, slatterned in vain, very unfortunate, can not satisfy the needs in the medical treatment.
The objective of the invention is to provide a kind of Processs for comprehensive recvery of blood, and according to this law, the blood recovery system that produces can separate and reclaim in the blood visible components such as cell, can separate again and reclaims useful composition in the blood plasma on demand.
The object of the present invention is achieved like this: this method is with effective various modern separation technology, comprising centrifuging, ultrafiltration membrance filter method, chromatography and electrophoresis method, according to the characteristics of each technology, planned combining, flow direction by various liquid in the on-off control blood, make blood in the process of each relevant devices of flowing through, from coarse to fine, with various different components, all first separation is come out, thereby reaches the purpose that reclaims and utilize.
The present invention is because of separating blood all sidedly in the disposable mode that flows through and reclaiming, so can be used for autotransfusion, single blood sampling, in the analysis and check of blood component displacement and blood component, the efficient height, and composition useful in the blood plasma is separated remain, be not wasted, remarkable economic efficiency and social benefit are arranged.
Particular content of the present invention is provided by following examples and accompanying drawing thereof:
Fig. 1 is the process flow sketch map of the inventive method.
Fig. 2 is the blood recovery system sketch map that adopts the inventive method to manufacture.
Fig. 3 is a blood recovery system B-B cross section cross-sectional schematic.
Fig. 4 is a blood recovery system A-A cross section cross-sectional schematic.
Describe details of the present invention in detail below in conjunction with accompanying drawing.
With reference to accompanying drawing 1～4, Processs for comprehensive recvery of blood of the present invention is that the operation recovery blood that is hung on the support 33 is put into blood microstrainer 8 from blood bag 1, to filter granule greater than 40 μ m, promptly sneak into impurity and blood clotting in the blood by the external world in the operation, start simultaneously anticoagulant pump 5 with anticoagulant from bag 2 in become 1: 7 ratio to pump in the blood microstrainer 8 with blood, carry out the anticoagulant processing to reclaiming blood, open normal saline switch 6 and blood switch 9, start blood pump 11, make an amount of normal saline enter separate cup 12 with mixing mutually through four-way 10 through blood microstrainer 8 filtering blood, start centrifuge 13, as required, adjust its rotating speed, make separate cup 12 rotations, blood in the cup after the dilution, under the action of the centrifugal, by the layering of density size, erythrocyte is the heaviest, at outermost layer, secondly be leukocyte, platelet, blood plasma.Because it is conical that separate cup top is, and when continuously importing blood, makes the erythrocyte layer progressive additive of being close to outer wall, promotes stratified blood plasma, platelet, leukocyte and discharge along outer field output channel from the bottom of separate cup successively.Open blood plasma switch 15, blood plasma enters blood plasma microstrainer 16 and filters, with various cell debriss of filtering and residual particles; Treat after the plasma flow, open switch 17, make platelet enter platelet collecting bag 18.Open switch 19 then, make leukocyte enter leukocyte collecting bag 20.During hot cells fill separate cup, close blood switch 9 and physiology reason salt taps 6, open erythrocyte and collect switch 7, and stop centrifuge and rotate, oppositely start blood pump 11 then, the erythrocyte of separator well is drawn in the erythrocyte collecting bag 4 from separate cup 12 by former input channel, so far finish first circulation hemocyte and separate, this mask work can handle as required blood volume what and carry out repeatedly repeatedly, till all operation loss blood is disposed.
Start blood plasma pump 21 then, blood plasma after will filtering through blood plasma microstrainer 16 pumps into chromatographic column 22, chromatographic column by containing different adsorbing materials is with the heterogeneity in the adsorbed plasma, it is fixed that the quantity of chromatographic column and the adsorbing material in the post come according to the material that will adsorb, adopt a chromatographic column in the present embodiment, adsorbing material adopts the DEAE cellulose, with the absorption thrombinogen.Like this, after plasma flow was crossed chromatographic column, thrombinogen wherein just was adsorbed material absorption, after whole flow of liquid, this chromatographic column is taken off, and uses the stripping liquid eluting, can obtain thrombinogen.Blood plasma is through flowing into the ultrafiltration membrance filter device or being called for short in the ultrafilter 23 behind the chromatographic column, the aperture of filter membrane and the quantity of filter membrane in this ultrafilter 23, decide according to required composition that leaches and kind, utilize the filter membrane in different apertures to collect in the blood plasma particle diameter greater than the various compositions in filter membrane aperture; Adopt two-stage filter in the present embodiment, the filter membrane aperture of first order filter is 70000 molecular weight, all molecular weight promptly are filtered off in the inflow globulin collecting bag 24 greater than 70000 globulin, the filter membrane aperture of second level filter is 50000 molecular weight, being mainly used in and leaching molecular weight is 67000 albumin, and the albumin that leaches flows in the albumin collecting bag 25.Flow into electrophoretic apparatus 26 through the liquid after the ultrafilter filtration, because of the nucleic acid in the liquid, protein, enzyme, polypeptide and aminoacid etc. all have ionogenic group, group can absorb in solution and become to provide hydrion and become charged particle, since in the blood plasma different components institute with the size of charged particle with more or less, so under the effect of electrophoresis tank internal electric field, charged particle to self electrically charged opposite electrode direction swimming, different distance place in electrophoresis tank like this, just assembling various different components, and can take out with known method, remaining moisture content just flows in the waste fluid bag 27.
The right side of anticoagulant flow meter 29 among Fig. 2, order is blood pump flow table, centrifuge speed table, blood plasma pump flow meter, electrophoretic voltage table; The right side of anticoagulant Flow-rate adjustment knob 28, order are and the corresponding various adjusting knobs of its various instrument in top.Key is corresponding with switch 6,7,9,15,17,19 respectively each minute in the button 30, and 31 is main switch, and 32 is display lamp.
Ultrafilter in the present embodiment, chromatographic column, and modern separation technology such as electrophoretic apparatus, be to take series connection method to be coupled to each other, also can be parallel with one another as required, simultaneously also can be on same operation the same isolation technics of parallel use, in addition, also can select wherein part isolation technics for use as required.
The main parts size specification of selecting for use among the embodiment is as follows:
1. high speed centrifuge (LG10-2.4A)
2. detachable blood composition separate cup (Shanghai medical apparatus and instruments special equipment factory)
3. infusion pump (SOP-II)
4. ultrafiltration membrance filter device (88-1) (military science medical college)
5. electrophoretic apparatus (DYY-III)
6. microstrainer (Xijing-1600)
Wherein blood microstrainer filter membrane aperture is 40 μ m
Blood plasma microstrainer filter membrane aperture is 20 μ m
The electromagnetic switch of using in chromatographic column and the blood recovery system is self-control, reclaims the machine shell and adopts the corrosion resistant plate manufacturing, and each parts all is fastened on the frame in the casing with screw.
Adopt this blood recovery system, through 20 minutes the 1000ml blood separation is gone out erythrocyte, leukocyte, platelet and plasma solutions, plasma solutions was isolated compositions such as globulin, albumin, thrombinogen, nucleic acid, polypeptide, creatine and bilirubin again through two hours.
Claims (2)
1, a kind of separation of blood and recovery method, special Processs for comprehensive recvery of blood is made of the isolation technics in integrated use modern times, it is characterized in that master operation is:
1) blood to be recycled and anticoagulant are put into blood microstrainer 8 simultaneously, to filter granule greater than 40 μ m;
2) will in normal saline is mixed into separate cup 12 mutually, utilize centrifuge that blood plasma, platelet, leukocyte, erythrocyte are isolated from microstrainer 8 effusive liquid, at last by oppositely starting blood pump, with erythrocyte in the separate cup in the collecting bag of falling the suction 4;
3) isolated blood plasma is pumped into chromatographic column 22, by containing the chromatographic column of different adsorbing materials, with the different components in the adsorbed plasma;
4) blood plasma by chromatographic column 22 enters in the ultrafiltration membrance filter device 23, utilizes the filter membrane in different apertures, and particle diameter is greater than the various compositions in filter membrane aperture in the collection blood plasma;
5) by the blood plasma behind the ultrafilter 23, enter in the electrophoretic apparatus 26, isolate useful composition after, discard institute's waste liquid.
2, Processs for comprehensive recvery of blood according to claim 1, it is characterized in that described modern separation technology, both can connect mutually as required, also can be parallel with one another, simultaneously also can be on same operation the same isolation technics of parallel use, in addition, also can select wherein part isolation technics for use as required.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN91103626A CN1055880A (en) | 1991-06-07 | 1991-06-07 | Processs for comprehensive recvery of blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN91103626A CN1055880A (en) | 1991-06-07 | 1991-06-07 | Processs for comprehensive recvery of blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1055880A true CN1055880A (en) | 1991-11-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN91103626A Pending CN1055880A (en) | 1991-06-07 | 1991-06-07 | Processs for comprehensive recvery of blood |
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| CN (1) | CN1055880A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103614339A (en) * | 2013-11-22 | 2014-03-05 | 武汉友芝友医疗科技有限公司 | Cell separation method |
| CN107693872A (en) * | 2017-11-01 | 2018-02-16 | 桂林欣中伊健康产业有限公司 | A kind of method of plasma purification |
| CN107693873A (en) * | 2017-11-01 | 2018-02-16 | 桂林欣中伊健康产业有限公司 | Plasma purification simple and easy method |
| CN112912725A (en) * | 2018-10-25 | 2021-06-04 | Tl基因体科技株式会社 | Pretreatment of blood to grade blood cells using microchannels |
-
1991
- 1991-06-07 CN CN91103626A patent/CN1055880A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103614339A (en) * | 2013-11-22 | 2014-03-05 | 武汉友芝友医疗科技有限公司 | Cell separation method |
| CN107693872A (en) * | 2017-11-01 | 2018-02-16 | 桂林欣中伊健康产业有限公司 | A kind of method of plasma purification |
| CN107693873A (en) * | 2017-11-01 | 2018-02-16 | 桂林欣中伊健康产业有限公司 | Plasma purification simple and easy method |
| CN112912725A (en) * | 2018-10-25 | 2021-06-04 | Tl基因体科技株式会社 | Pretreatment of blood to grade blood cells using microchannels |
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