CN105586349A - Drought-resistant salt-tolerant gene TaPIP1A of wheat and encoded protein and application of TaPIP1A - Google Patents
Drought-resistant salt-tolerant gene TaPIP1A of wheat and encoded protein and application of TaPIP1A Download PDFInfo
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Abstract
The invention discloses a drought-resistant salt-tolerant gene TaPIP1A of wheat and encoded protein and application of the TaPIP1A. The nucleotide sequence of the drought-resistant salt-tolerant gene TaPIP1A of the wheat is shown as SEQ ID NO:1; the gDNA sequence is shown as SEQ ID NO:2; the amino acid sequence of the encoded protein is shown as SEQ ID NO:3. The invention further discloses recombinant plasmid containing the drought-resistant salt-tolerant gene TaPIP1A of the wheat and application of the gene TaPIP1A in cultivating drought-resistant slat-tolerant plant varieties. According to the drought-resistant salt-tolerant gene TaPIP1A of the wheat and the encoded protein and application of the TaPIP1A, a plant gene engineering technology is applied, the gene TaPIP1A is obtained from wheat Kn9204 for the first time, it is proved through experiments that the gene TaPIP1A is transferred into arabidopsis through an agrobacterium-mediated transformation method, and transgenic arabidopsis plants have higher salt-tolerant and drought-resistant capacity; a candidate gene is provided for development and cultivation of drought-resistant salt-tolerant new varieties of crops, and very important significance is achieved for cultivating more new varieties of drought-resistant salt-tolerant plants.
Description
Technical field
The present invention relates to technical field of biological genetic engineering, specifically a kind of Drought-resistance in Wheat, resistant gene of salt TaPIP1AAnd encoding proteins and application.
Background technology
Global water resources shortage at present, under limited land resource prerequisite, effectively farming seems particularly important.Abiotic stress is larger to the yield effect of crops, and in various abiotic stress, drought is coerced with salt stress and often caused and plantThing cell dehydration, ionic equilibrium are broken, and then cause crop yield to reduce, and this just requires the raw plant of anchor root in the face of variousExtraneous environment stress factor, must possess with Stress Factors adapt stress mechanism to reduce stress damage. Therefore, plant existsVarious to coerce down the mechanism that maintains its water balance be the focus of degeneration-resistant research always, is also that to cultivate salt tolerant by genetic engineering anti-The Research foundation of dry farming thing new varieties.
Aquaporin is the AQP-CHIP that a class is efficiently transported hydrone, rises emphatically in plant moisture transfer processAct on. Moisture transports three kinds of approach in plant: apoplast approach, symplast approach and transcellular pathway. MoistureCross-film transport has three kinds of modes: by the free diffusing transport of lipid bilayer (after birth), transport by protein called membrane transporters with pass throughThe passive transport of aquaporin. Conventionally the studies on plant aquaporins is divided at present to four classes: be positioned at the inherent egg of plasma membrane on plasma membraneWhite PIPs, be positioned at tonoplast intrinsic protein TIPs on tonoplast, be present in symbiosis root nodule bacteroid around Nod26 class on film,Little molecule alkalescence integral protein SIPs. At present, people have done some relevant grinding to the aquaporin of the crops such as tobaccoStudy carefully, but the research of the aquaporin function and effect mechanism of wheat is little. Wheat be the important cereal crops in the whole world itOne, its drought resisting is or/and salt tolerant research receives people's concern day by day. Therefore, research wheat itself and the side of body under Drought and salt is coercedUrgent factor adapt stress mechanism related gene, and utilize related gene characteristic cultivate wheat and other plant droughts resistance toSalt new varieties, are of great significance agricultural development tool.
Summary of the invention
The object of this invention is to provide a kind of Drought-resistance in Wheat, resistant gene of salt TaPIP1A and encoding proteins and application, is agricultureResearch and the cultivation of crop drought resistance New salt-tolerant cultivar provide candidate gene, to cultivate more drought resistance and salt tolerance neies variety of plant.
The present invention is achieved in that a kind of Drought-resistance in Wheat, resistant gene of salt TaPIP1A, the cDNA of described gene TaPIP1ASequence is as shown in SEQIDNO:1; The gDNA sequence of described gene TaPIP1A as shown in SEQIDNO:2, its gDNA by3 extrons and 2 introne compositions; Since 5 ' end, the length of extron is followed successively by 642bp, 141bp, 96bp, introneLength is followed successively by 129bp, 116bp.
The invention also discloses the encoding proteins of a kind of Drought-resistance in Wheat, resistant gene of salt TaPIP1A, its amino acid sequence asShown in SEQIDNO:3; The encoding proteins of this gene TaPIP1A is a kind of wheat aquaporin that is positioned cytoplasma membraneMatter.
The invention also discloses a kind of recombinant plasmid, described recombinant plasmid comprises Drought-resistance in Wheat claimed in claim 1, resistance toSalt gene TaPIP1A; The carrier of this plasmid is preferably pCAMBIA1300, and recombinant plasmid is preferably pCAMBIA1300-TaPIP1A, in addition, anyly can import foreign gene in the carrier of expressing in plant and may be used to the present invention.
Drought-resistance in Wheat provided by the invention, the application of resistant gene of salt TaPIP1A in seed selection drought resistance and salt tolerance plant variety, shouldPlant preferably wheat or arabidopsis.
Concrete application process is: by described Drought-resistance in Wheat, resistant gene of salt TaPIP1A or will contain Drought-resistance in Wheat, salt tolerantThe recombinant plasmid of gene TaPIP1A imports in cell, tissue or the organ of host's wheat or arabidopsis, and cultivation obtains having anti-Drought, Salt-tolerant Wheat or arabidopsis new varieties. For the ease of genetically modified plants or clone are screened, can be to described in containingThe plant expression vector pCAMBIA1300-TaPIP1A of gene TaPIP1A processes, and the mark that can bring Selection In, as GUSDeng. .
Agriculture 9204 (KN9204) of the present invention section used, for authorization kind, examined by Hebei province's variety of crops in 2002Determine committee's authorization; Within 2003, by national variety certification, variety certification is numbered state and examines wheat 2003037.
The present invention has applied plant gene engineering technology, obtains gene TaPIP1A first from wheat section agriculture 9204, passes throughExperiment showed, this gene TaPIP1A by agriculture bacillus mediated method by this gene transferred plant (arabidopsis), this transgenosisCan make plant (arabidopsis) have stronger drought resistance and salt tolerance ability, this is research and the cultivation of crops salt-tolerant drought-resistant new varietiesGood candidate gene is provided, has been of great significance realizing cultivation drought resistance and salt tolerance new variety of plant tool.
Brief description of the drawings
Fig. 1 is the amplification of cDNA sequence and the gDNA sequence of wheat cdna TaPIP1A. M is DNAMarkerIII(Tian Gen biochemical technology Co., Ltd, Beijing).
Fig. 2 is the Molecular Detection result of transgenosis TaPIP1A arabidopsis positive plant.
Fig. 3 is the phenotypic evaluation result of transgenosis TaPIP1A arabidopsis in Their Seed Germinating Period salt tolerance and drought tolerance.
Fig. 4 is that transgenosis TaPIP1A arabidopsis is coerced lower seed germination rate statistics at Drought and salt.
Fig. 5 is the Salt-Tolerance Identification result of transgenosis TaPIP1A arabidopsis in seedling stage.
Detailed description of the invention
Experimental technique in following embodiment, if no special instructions, is conventional method. Test material used in embodimentMaterial, reagent etc., if no special instructions, all can obtain from commercial channels. Quantitative test in following examples, all arranges weight three timesMultiple experiment, results averaged. M in literary composition represents mol/L.
The clone of embodiment 1 wheat TaPIP1A gene
1, extract the total RNA of wheat
(1) organization material of section's agriculture 9204 is put into the mortar of Liquid nitrogen precooler, abundant grind into powder in liquid nitrogen;
(2) treat that liquid nitrogen volatilization is dry, transfer to immediately in the centrifuge tube of 2ml, every 100mg material approximately adds the Invitrogen of 1mlThe Trizol extract of company, after thawing, repeatedly inhales and blows with sample loading gun, and thermal agitation mixes sample, makes its abundant cracking, room temperaturePlace 5 minutes;
(3) add 0.2ml chloroform (chloroform), thermal agitation mixes 15 seconds, and room temperature is placed 10 minutes; At 4 DEG C,Centrifugal 15 minutes of 12000rpm;
(4) careful sucking-off upper strata water, joins in the centrifuge tube of clean 1.5ml, adds the isopropyl alcohol (upper water of 500 μ lWith the volume ratio of isopropyl alcohol be 1:1), fully mix, at-20 DEG C, precipitation 30min; At 4 DEG C, 12000rpm is centrifugal10min, careful abandoning supernatant, stays precipitation;
(5) the ethanolic solution washing that is 75% by precipitation with the concentration of volume percent of 1ml, at 4 DEG C, 8000rpm centrifugal 10Min, collects RNA precipitation;
(6) RNA is deposited on aseptic operating platform and dries about 10-15 minute, when RNA shows slightly transparent, adds the RNase-of 50 μ lFree water fully dissolves, and can be placed at-80 DEG C and preserves for a long time, for subsequent use;
(7) the Agrose detected through gel electrophoresis RNA concentration and the quality that are 1% with ultraviolet specrophotometer and mass percent concentration.
2, cDNA reverse transcription
Carry out according to the description of RNAPCRKit (AMV) Ver.3.0 kit (TaKaRa, DRR019A); First carry outFirst step reverse transcription reaction, carries out reverse transcription taking the RNA of 500ng as template, indicates in reaction system according to described descriptionIn add successively by MgCl2、10×RTbuffer、RNaseFreedH2O、dNTPMixture、RNaseInhibitor、The reaction system totally 10 μ L of AMVReverseTranscriptase, OligodTPrimer and TotalRNA composition. InsteadThe program of answering is: 42 DEG C of 30min, 99 DEG C of 5min, 5 DEG C of 5min.
3, clone and sequencing
(1), taking cDNA as template, at 5 ' UTR and 3 ' UTR design gene specific primer, primer sequence is respectively:
Upstream primer XbaI-PIP1A:5 '-GCTCTAGATCTCTCCCAAAGCCAAGAGC-3 ', downstream primer BamHI-PIP1A：5’-CGGGATCCCAGCGATAAGATTCCACCCG-3’；
(2) PCR reaction system (totally 20 μ L):
2×TaqPCRMasterMix10μL
Upstream primer XbaI-PIP1A0.5 μ L
Downstream primer BamHI-PIP1A0.5 μ L
Template cDNA1 μ L
ddH2O8μL
(3) PCR response procedures: 94 DEG C of denaturation 5min; 94 DEG C of sex change 30sec, 58 DEG C of annealing 30sec, 72 DEG C are extended 1Min15s, 30 circulations/min; 72 DEG C are extended 7min; 20 DEG C of preservations, the specific amplified band that amplification obtains is as shown in Figure 1;
Reclaim PCR product and connect pEASY-Bluntcloningvector(Quan Shijin Bioisystech Co., Ltd) surveyOrder, the nucleotides sequence of the PCR product that amplification obtains is classified SEQIDNO:1 as, and its initiation codon is large to terminator codonLittle is 879bp, and the unnamed gene of this PCR product is TaPIP1A, the amino acid sequence of the encoding proteins of this gene TaPIP1A asShown in SEQIDNO:3.
Embodiment 2TaPIP1A genome gDNA full-length clone
(1) gDNA extracts: extract kit description (Tian Gen biochemical technology Co., Ltd, Beijing) method according to plant gDNA and carryGet section's agriculture 9204 wheat gDNA;
(2), taking the gDNA of section's agriculture 9204 as template, at 5 ' UTR and 3 ' UTR design gene specific primer, upstream and downstream primer is as follows:
Upstream primer XbaI-PIP1A:5 '-GCTCTAGATCTCTCCCAAAGCCAAGAGC-3’，
Downstream primer BamHI-PIP1A:5 '-CGGGATCCCAGCGATAAGATTCCACCCG-3’；
Carry out pcr amplification, amplification condition is with the increase operating procedure of cDNA of embodiment 1. By the specific amplified band obtaining as Fig. 1Shown in, connect pEASY-Bluntcloningvector order-checking, obtain the total length of gene TaPIP1A in section's agriculture 9204GDNA sequence, from initiation codon to terminator codon, size is 1124bp.
Because the ORFs size of cDNA in embodiment is 879bp, its gDNA is from initiation codon to termination codonThe size of son is also 1124bp, compares demonstration by nucleotide sequence, and the gDNA of gene TaPIP1A is by 3 extrons and 2Introne composition; Since 5 ' end, the length of extron is followed successively by 642bp, 141bp, 96bp, and length of intron is followed successively by129bp、116bp。
The acquisition of embodiment 3 recombinant plasmid pCAMBIA1300-TaPIP1A
(1) by restriction enzyme Xba I and BamH I to obtained the PCR product (gene that molecular weight is 879bp by embodiment 1TaPIP1A) carry out double digestion, its double digestion system is: Xba I 1 μ l, BamH I 1 μ l, 10 × bufferK1 μ l, PCR product 5 μl，ddH2O12 μ l, the enzyme that obtains TaPIP1A gene is cut product;
(2) use restriction endonuclease Xba I and BamH I to plasmid pCAMBIA1300 double digestion, its double digestion system: Xba I 1 μ l, BamH I 1μ l, 10 × bufferK1 μ l, plasmid 5 μ l, ddH2O12 μ l, the enzyme that obtains pCAMBIA1300 is cut product;
(3) enzyme of TaPIP1A gene is cut to product respectively and carrier restriction enzyme enzyme at 37 DEG C is cut 3-5h, afterwardsAt 65 DEG C, 20min makes restriction enzyme enzyme deactivation, and the enzyme of object fragment TaPIP1A gene is cut to product and carrierThe enzyme of pCAMBIA1300 cut product be in molar ratio 5:1 mix, add simultaneously 1 μ LT4DNAligase and 2 μ L10 ×T4DNAligasebuffer, adds water to 20 μ L; 4 DEG C of connections of spending the night; Obtain recombinant plasmid pCAMBIA1300-TaPIP1A, rear conversion bacillus coli DH 5 alpha competent cell (Tian Gen biochemical technology Co., Ltd, Beijing), qualification recombinant plasmidAfter obtain the double source expression vector with genes of interest.
(4) qualification of recombinant plasmid: have the clone of Insert Fragment with the screening of bacterium colony PCR method, concrete operations are as follows:
1. picking transform after white colony on flat board, draw short-term, 37 DEG C to be cultured to bacterium line visible, carries out bacterium colony PCR reaction;
2. scrape with toothpick the thalline taking a morsel and proceed to 20 μ l containing in the PCR system of described primer, carry out PCR reaction. PCR reactionSystem: 2 × TaqPCRMasterMix10 μ L, upstream primer XbaI-PIP1A1 μ L, downstream primer BamHI-PIP1A1μl、ddH2O8 μ L, PCR response procedures is: 94 DEG C of denaturation 5min; 94 DEG C of sex change 30sec, 58 DEG C of annealing 30sec, 72DEG C extend 1min15s, 30 circulations/min; 72 DEG C are extended 7min; 20 DEG C of preservations;
3. PCR product, in 0.8% agarose gel electrophoresis, detects the fragment that whether contains 879bp molecular size range, and checking is carriedBody is correct structure, after qualification recombinant plasmid, obtains the plant expression vector with genes of interest.
Recombinant plasmid is sent to order-checking, result be this recombinant plasmid be by described in SEQIDNO:1 from 5 ' end1-879 position nucleotides inserts between the Xba I and BamH I restriction enzyme site of pCAMBIA1300, names this recombinant plasmid to bepCAMBIA1300-TaPIP1A。
Recombinant plasmid is proceeded to Agrobacterium GV3101 competence by embodiment 4
(1) cultivate Agrobacterium GV3101: the single colony inoculation of picking Agrobacterium is in 3mLYEB(60mg/Lrif) fluid nutrient mediumIn, 28 DEG C are shaken training and spend the night; Get 500 μ L and be inoculated in 50mLYEB(60mg/Lrif) in fluid nutrient medium, 28 DEG C are shaken training extremelyOD600Be 0.6; Bacterium liquid is proceeded in 50mL centrifuge tube to ice bath 30min; 4 DEG C, 5000g, centrifugal 5min; Abandon supernatant, precipitationResuspended with the NaCl of 10mL0.15M; 4 DEG C, 5000g, centrifugal 5min; Abandon supernatant, the CaCl of 1mL20mM for precipitation2HeavyOutstanding; Every pipe 100 μ L/ pipe packing; Liquid nitrogen frozen 5min;-70 DEG C of preservations;
(2) proceeding to of recombinant plasmid: recombinant plasmid pCAMBIA1300-TaPIP1A prepared by 10 μ L embodiment 3 adds 100In μ L Agrobacterium GV3101 competence, mix; Ice bath 30min successively, liquid nitrogen frozen 3-5min, 37 DEG C of water-bath 5min; Add1mLYEB fluid nutrient medium, 28 DEG C are slowly shaken training 2-4h; 4 DEG C, 5000rpm, centrifugal 5min; Abandon part supernatant, in residueAfter clear resuspended thalline, be applied to YEB(60mg/Lrif) on solid medium, cultivate 2-3 days for 28 DEG C;
(3) thalline PCR qualification, chooses positive colony and obtains the Agrobacterium of recombinating, and its concrete authentication step is with step (4) in embodiment 3,Obtain the Agrobacterium GV3101 that contains recombinant plasmid.
Screening and the acquisition of embodiment 5 transgenic arabidopsis
(1) plantation of arabidopsis: (every 100mL sterilization mixed liquor contains 7.5g by sterilization mixed liquor for wild type arabidopsis seedClorox and the Triton-X100 of 0.01g) sterilization 15 minutes, then use rinsed with sterile water 5-6 time, put and be sowed at MS flat boardUpper, in 4 DEG C of vernalization 2-3 days; Then be transplanted to (volume ratio of Nutrition Soil and vermiculite is 1:1) in nutritive cube 23 DEG C of cultivations, 16/8The h photoperiod, light intensity 30-40 μ molm-2s-1; After plant to be planted is bloomed, cut off its major branch top, promote side shoot development; After beta pruning4-6 days in, transform;
(2) transform: in test tube, YEB fluid nutrient medium shakes the Agrobacterium GV3101 containing recombinant plasmid that training embodiment 4 obtains, 28DEG C spend the night; The Agrobacterium GV3101 containing recombinant plasmid that shakes training by spending the night for the ratio of 1:100 by volume spreads cultivation in 100mLIn YEB fluid nutrient medium, to OD600Be about 1.0; Room temperature, 5500g, centrifugal 10min collection bacterium; Thalline is resuspended in to conversion mediumIn, adjust OD600Be about 0.8; The conversion medium of Agrobacterium GV3101 containing recombinant plasmid is poured in container, by the plan choosingSouth mustard is inverted (conversion is front cuts off angle fruit and wide-open bud, only stays and just shows money or valuables one carries unintentionally and young tender bud) on it and soaksBubble 5min; Take out arabidopsis, lie on one's side and be placed in clean plastic tub, and cover lucifuge moisturizing renewal cultivation 16-with black thin film24 hours; Open film, arabidopsis is placed under light, normally cultivate;
(3) screening of positive plant: T0For (the hypochlorous acid that every 100mL sterilization mixed liquor contains 7.5g of sterilization mixed liquor for seedThe Triton-X100 of sodium and 0.01g) sterilization is after 15 minutes, and program request is selected on culture plate (25mg/L hygromycin) at MS; In 4Vernalization 2-3 days at DEG C; Move in culturing room and cultivate; Cultivate 10 days, (grow true leaf 1-2 couple, root is stretched to select hygromycin resistance plantGrow in culture medium) and be transplanted in nutritive cube; Cultivate until seed maturity; Same method screening T1Obtain T for seed2Dai ZhiStrain; And at T2Insert independent strain for selecting resistance in plant than the single copy for 3:1, and obtain the T that isozygotys3For strain OE1, OE6And OE12;
(4) the arabidopsis Molecular Detection of transgenosis TaPIP1A: OE1, the OE6 to above-mentioned acquisition and OE12 carry out transgenosis and intend southThe Molecular Detection of mustard, taking the gDNA of transformation plant as template, is used gene specific primer to carry out Genomic PCR, positive gram of qualificationGrand, its amplimer and PCR condition are with 3 step (1) and (2) in example 1; Its amplification is shown in Fig. 2; Fig. 2 amplification tableBright transgenic line OE1, OE6 and OE12 have amplified target sizes band, and wild type contrast (WT) does not amplify band.
Embodiment 6 transgenosis TaPIP1A Seed Germination of Arabidopsis Pumila salt tolerants and drought tolerance qualification
By wild type arabidopsis WT and T3For single seed sterilization mixed liquor that copies isozygoty arabidopsis strain OE1, OE6 and OE12(clorox that every 100mL sterilization mixed liquor contains 7.5g and the Triton-X100 of 0.01g) sterilization 15 minutes, then uses nothingBacterium water rinse 5-6 time, selects and is sowed at the mannital(sweet mellow wine simulating drought containing 300mM) or flat containing the MS of the NaCl of 200mMOn plate, in 4 DEG C of vernalization 2-3 days, 23 DEG C of cultivations, 16/8h photoperiod, light intensity 30-40 μ molm-2s-1; With similarity condition without interpolationCoercing material is blank, observes seed germination situation. Seed germination the results are shown in Figure 3. In figure, OE1, OE6 and OE12 represent to turnGene arabidopsis strain, WT represents wild type arabidopsis, control represents not carry out any blank seed of coercing and sproutsThe situation of sending out. The arabidopsis that proceeds to wheat cdna TaPIP1A can be obviously found out in contrast from figure, 300mM'sUnder the Drought and salt stress conditions of the NaCl simulation of mannital or 200mM, seed germination situation is significantly better than wild type pairAccording to; Blank and arid (mannital) or salt (NaCl) are coerced to lower wild type (WT) and transgenic arabidopsis OE1, OE6Add up with OE12 seed germination rate, its germination rate exposes little Bai bud as foundation taking seed, cultivates 15 days statistics afterwards, statisticsAs Fig. 4, show that from data result transgenosis TaPIP1A arabidopsis plant is remarkable containing the germination rate sweet mellow wine or salt culture mediumHigher than wild type, show that the drought-enduring and salt ability of transgenosis TaPIP1A arabidopsis obviously increases.
Embodiment 6 transformed plant Seedling Salt-tolerance qualifications
By wild type arabidopsis WT and T3For single seed sterilization mixed liquor that copies isozygoty arabidopsis strain OE1, OE6 and OE12(clorox that every 100mL sterilization mixed liquor contains 7.5g and the Triton-X100 of 0.01g) sterilization 15 minutes, then uses nothingBacterium water rinse 5-6 time, point is sowed on MS flat board, in 4 DEG C of vernalization 2-3 days, 23 DEG C of cultivations, 16/8h photoperiod, light intensity 30-40 μmolm-2s-1, after 10 days, seedling replanting is entered to vermiculite and carries out earth culture; After 3 weeks of earth culture, carrying out concentration is the NaCl place of 300mMManage 8 days. Plant strain growth situation is shown in Fig. 5. Fig. 5 result shows, transgenosis TaPIP1A arabidopsis is compared with contrasting wild type, salt tolerantAbility obviously strengthens.
In sum, gene TaPIP1A is proceeded in plant and can make plant there is stronger drought resisting and the performance of salt tolerant.Foregoing description only proposes as the enforceable technical scheme of the present invention, not as the Single restriction condition to its technical scheme itself.
Claims (7)
1. Drought-resistance in Wheat, a resistant gene of salt TaPIP1A, is characterized in that, the cDNA sequence of described gene TaPIP1A is as SEQShown in IDNO:1 or gDNA sequence as shown in SEQIDNO:2.
2. an encoding proteins of Drought-resistance in Wheat, resistant gene of salt TaPIP1A, is characterized in that, its amino acid sequence is as SEQIDShown in NO:3.
3. a recombinant plasmid, is characterized in that, described recombinant plasmid comprises Drought-resistance in Wheat claimed in claim 1, salt tolerant baseBecause of TaPIP1A.
4. recombinant plasmid according to claim 3, is characterized in that, described recombinant plasmid is pCAMBIA1300-TaPIP1A。
5. a Drought-resistance in Wheat, resistant gene of salt TaPIP1A are in the application of cultivating in drought resisting, salt-tolerant plant kind.
6. Drought-resistance in Wheat according to claim 5, resistant gene of salt TaPIP1A are cultivating in drought resisting, salt-tolerant plant kindApplication, is characterized in that, by described Drought-resistance in Wheat, resistant gene of salt TaPIP1A or will contain Drought-resistance in Wheat, resistant gene of saltThe recombinant plasmid of TaPIP1A imports in cell, tissue or the organ of host plant, cultivates and obtains drought resisting, salt-tolerant plant new varieties.
According to the Drought-resistance in Wheat described in claim 5 or 6, resistant gene of salt TaPIP1A cultivating in drought resisting, salt-tolerant plant kindApplication, it is characterized in that, described plant is wheat or arabidopsis.
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Cited By (2)
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| CN113308489A (en) * | 2021-06-18 | 2021-08-27 | 中国农业科学院生物技术研究所 | Creation method of novel salt-tolerant oat germplasm |
| CN116656698A (en) * | 2023-07-27 | 2023-08-29 | 河南大学三亚研究院 | Application of corn gene Zm00001d018037 in improving drought resistance of monocotyledonous crops |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113308489A (en) * | 2021-06-18 | 2021-08-27 | 中国农业科学院生物技术研究所 | Creation method of novel salt-tolerant oat germplasm |
| CN116656698A (en) * | 2023-07-27 | 2023-08-29 | 河南大学三亚研究院 | Application of corn gene Zm00001d018037 in improving drought resistance of monocotyledonous crops |
| CN116656698B (en) * | 2023-07-27 | 2023-11-03 | 河南大学三亚研究院 | Application of corn gene Zm00001d018037 in improving drought resistance of monocotyledonous crops |
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