CN105586254A - Microbionation device and microbionation method thereof - Google Patents
Microbionation device and microbionation method thereof Download PDFInfo
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- CN105586254A CN105586254A CN201610165734.4A CN201610165734A CN105586254A CN 105586254 A CN105586254 A CN 105586254A CN 201610165734 A CN201610165734 A CN 201610165734A CN 105586254 A CN105586254 A CN 105586254A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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Abstract
The invention provides a microbionation device and belongs to the technical field of microculture. The microbionation device comprises an interface and a soft inoculation rod, the top end of the soft inoculation rod is connected to the bottom of the interface, the bottom end of the soft inoculation rod is provided with a smooth round end, the outer surface of the soft inoculation rod is provided with a groove, the groove is formed in the axial direction of the soft inoculation rod, the middle and upper portion of the soft inoculation rod is provided with an easily-bent ring, the easily-bent ring is arranged in the circumferential direction of the soft inoculation rod, and the diameter of the easily-bent ring is smaller than that of the soft inoculation rod. The invention further provides a microbionation method through the microbionation device. The microbionation device can serve as an inoculating loop for plate streaking inoculation and can also serve as a spreader for spread inoculation and is safe, sterile, convenient, simple, practical, skilled, simple in structure and low in production cost.
Description
Technical field
The present invention relates to microorganism culture technique field, specifically a kind of microbionation device.
Background technology
At present, in clinical bacterial analysis etc. is tested the analysis of microorganism, bacterium liquid need to be seeded inOn the surface of culture dish, then by observation, analytical equipment etc., it is carried out to scientific experiment.
In microbe research, the culture medium microbionation device instrument that is absolutely necessary. But, have at present twoKind, one is oese: existing oese comprises nickel filament, metal handle, insulation handle three parts, nickelOn chromium silk, have a screw socket, structure as shown in Figure 1, 2; Another is glass spatula (being again spreading rod),Though be glass material or metal material be triangle spreading rod, comprise triangle spatula district andHandle, the angle between handle and triangle spatula district place plane is 120 °-140 °, structure is as Fig. 3 instituteShow. Existing have following shortcoming for oese and the spreading rod inoculated: (1) function singleness, and existingSome oeses can only be used for streak inoculation, and can not be used for being coated with inoculation, and existing spreading rod can notComplete the function of oese; (2) while carrying out microbionation, need to carry out high temperature sintering sterilizing, cause temperatureSpend height and thalline is scalded dead; (3) inconvenient operation, cost of manufacture is high.
Summary of the invention
In view of this, the present invention aims to provide a kind of microbionation device, both can be used as oese and put downPlate streak inoculation, can be used as again spreader and be coated with inoculation; Safety asepsis; Convenient, simple, realWith, skilled; Simple in structure, cost of manufacture is cheap.
For achieving the above object, technical scheme of the present invention is achieved in that a kind of microbionation device,Comprise an interface and one inoculation soft bar, the top of the soft bar of described inoculation is connected in the bottom of interface, described in connectThe bottom of planting soft bar is provided with smooth round end, and the outer surface of the soft bar of described inoculation is provided with groove, described groove edgeInoculate the axial setting of soft bar, the middle and upper part of the soft bar of described inoculation is provided with an easy curved circle, described easy curved circle edgeInoculate the circumferential setting of soft bar, the diameter of described easy curved circle is less than the diameter of inoculating soft bar, and the present invention both canCarry out plate streaking inoculation to be used as oese, slightly firmly will inoculate soft bar simultaneously and bend, can be used as againSpreader is coated with inoculation.
Further, described groove extends to smooth round end by the top of inoculating soft bar, and groove can be retainedInoculation liquid.
Further, described groove is uniformly distributed in the outer surface of the soft bar of inoculation.
Further, the distance between adjacent notches equates.
Further, the diameter that is positioned at the soft bar of inoculation of easy curved circle top reduces from top to bottom successively.
Further, described interface, the material of inoculating soft bar and smooth round end are high-temperature resistance plastice, substituteTraditional glass material or metal material, cost of manufacture is cheap, carrying out bacterium when inoculation coating does not needTo carry out again high temperature sintering sterilizing, avoid excess Temperature and thalline is scalded dead.
Further, the material of described interface is polypropylene.
Further, the material of the soft bar of described inoculation and smooth round end is polytetrafluoroethylene (PTFE).
The present invention also provides above-mentioned microbionation device to carry out the method for microbionation:
(1), in the time need to exercising the function of oese, dip bacterium liquid or bacterium colony carries out with smooth round endInoculation, groove can be retained inoculation liquid, can increase qualitatively or reduce inoculum concentration, selects according to inoculum concentrationSelect the length of the soft bar immersed in liquid level of inoculation;
(2) in the time need to exercising the function of spreading rod, slightly firmly microbionation device is oliquely downward pressed,Inoculate soft bar in easy curved circle place's bending, groove can be retained inoculation liquid, inoculation liquid can be transferred to otherPlace, what in the time of coating, bacterium liquid distributed is more even.
The present invention also provides a kind of microbionation device, comprises a pipettor and a microbionation device, instituteState microbionation device and comprise an interface and the soft bar of an inoculation, the top of the soft bar of described inoculation is connected in interfaceBottom, the bottom of the soft bar of described inoculation is provided with smooth round end, and the outer surface of the soft bar of described inoculation is provided with groove,Described groove is along the axial setting of the soft bar of inoculation, and the middle and upper part of the soft bar of described inoculation is provided with an easy curved circle, instituteState the circumferential setting of easy curved circle along the soft bar of inoculation, the diameter of described easy curved circle is less than the diameter of inoculating soft bar,Described pipettor and interface removably connect.
Further, the end of described pipettor is provided with a suction nozzle, and described suction nozzle and interface removably connect,The interface of microbionation device top is docked with the suction nozzle of pipettor, can easily connect or lay down, to moveLiquid device is that carrier is inoculated, convenient, simple, practical, skilled.
With respect to prior art, the present invention is convenient, simple and easy, safety asepsis, two-in-one microbionation deviceThere is following advantage:
(1) multifunctionality: microbionation device of the present invention both can be used as oese and carry out plate streaking and connectKind, slightly firmly will inoculate soft bar simultaneously and bend, can be used as again spreader and be coated with inoculation;
(2) safety asepsis: microbionation device of the present invention can be placed on sterilization cassette as traditional suction nozzleIn, carry out high pressure steam sterilization, avoid the possibility of microbiological contamination; Meanwhile, need not in the time carrying out microbionationCarry out again high temperature sintering sterilizing, exempt the cooling stand-by period and avoid excess Temperature that thalline is scalded dead;
(3) convenience: the interface of microbionation device of the present invention top docks with the suction nozzle of pipettor, canEasily connect or lay down, inoculating taking pipettor as carrier, convenient, simple, practical, skilled;
(4) simplification: microbionation device of the present invention is compared with spreading rod with traditional oese, structureSimply, make with high-temperature resistance plastice, cost of manufacture is cheap.
Brief description of the drawings
The accompanying drawing that forms a part of the present invention is used to provide a further understanding of the present invention, of the present inventionSchematic description and description is used for explaining the present invention, does not form inappropriate limitation of the present invention. ?In accompanying drawing:
Fig. 1 is the structural representation of prior art oese.
Fig. 2 is the structural representation of prior art oese.
Fig. 3 is the structural representation of prior art spreading rod.
Fig. 4 is the structural representation of microbionation device of the present invention.
Fig. 5 is the local enlarged diagram of microbionation device of the present invention.
Structural representation when Fig. 6 is microbionation device enforcement spreading rod function of the present invention.
Fig. 7 is the structural representation of microbionation device of the present invention.
In figure: 1, interface; 2, easy curved circle; 3, inoculate soft bar; 4, groove; 5, smooth round end; 6,Pipettor; 7, suction nozzle.
Detailed description of the invention
It should be noted that, in the situation that not conflicting, the spy in embodiment and embodiment in the present inventionLevy and can mutually combine.
In description of the invention, it will be appreciated that, term " " center ", " longitudinally ", " laterally "," on ", D score, 'fornt', 'back', " left side ", " right side ", " vertically ", " level ",Orientation or the position relationship of the instruction such as " top ", " end ", " interior ", " outward " are based on shown in accompanying drawingOrientation or position relationship, be only the present invention for convenience of description and simplified characterization, instead of instruction or darkThe device or the element that show indication must have specific orientation, construct and operation with specific orientation, thereforeCan not be interpreted as limitation of the present invention. In addition, term " first ", " second " etc. are only for describingObject, and can not be interpreted as instruction or hint relative importance or implicit indicate indicated technical charactericticQuantity. Thus, the feature that is limited with " first ", " second " etc. can be expressed or impliedly wrapDraw together one or more these features. In description of the invention, except as otherwise noted, " multiples' "Implication is two or more.
In description of the invention, it should be noted that, unless otherwise clearly defined and limited, term" installation ", " being connected ", " connection " should be interpreted broadly, and for example, can be to be fixedly connected with, alsoCan be to removably connect, or connect integratedly; Can be mechanical connection, can be also electrical connection; CanTo be to be directly connected, also can indirectly be connected by intermediary, can be the connection of two element internals.For the ordinary skill in the art, can understand above-mentioned term in the present invention by concrete conditionIn concrete meaning.
Describe below with reference to the accompanying drawings and in conjunction with the embodiments the present invention in detail.
As shown in Figure 4,5, a kind of microbionation device, comprises an interface 1 and the soft bar 3 of an inoculation, inoculates softThe top of bar 3 is connected in the bottom of interface 1, and the bottom of inoculating soft bar 3 is provided with smooth round end 5, inoculates soft bar3 outer surface is provided with groove 4, and groove 4 is along the axial setting of the soft bar 3 of inoculation, and groove 4 is by the soft bar 3 of inoculationTop extends to smooth round end 5, and groove 4 is uniformly distributed in the outer surface of the soft bar 3 of inoculation, adjacent notches 4 itBetween distance equate, groove 4 can be retained inoculation liquid, the middle and upper part of inoculating soft bar 3 is provided with an easy curved circle 2,Easy curved circle 2 is along the circumferential setting of the soft bar 3 of inoculation, and the diameter of easy curved circle 2 is less than the diameter of inoculating soft bar 3, positionDiameter in the soft bar 3 of inoculation of easy curved circle 2 tops reduces from top to bottom successively, and the material of interface 1 is poly-thirdAlkene, the material of inoculating soft bar 3 and smooth round end 5 is polytetrafluoroethylene (PTFE), substituted traditional glass material orPerson's metal material, cost of manufacture is cheap, does not need to carry out high temperature sintering again and go out while carrying out bacterium coating inoculationBacterium, exempts the cooling stand-by period and avoids excess Temperature that thalline is scalded dead; The present invention both can be used as and connectPlant ring and carry out plate streaking inoculation, slightly firmly will inoculate soft bar simultaneously and bend, can be used as again spreader and enterRow coating inoculation.
As shown in Figure 7, a kind of microbionation device, comprises a pipettor 6 and a microbionation device, moves liquidThe end of device 6 is provided with a suction nozzle 7, and suction nozzle 7 removably connects with the interface 1 of microbionation device, and bacterium is connectThe interface 1 of planting device top docks with the suction nozzle 7 of pipettor 6, can easily connect or lay down, with pipettor 6For carrier is inoculated, convenient, simple, practical, skilled.
The course of work of the present embodiment: before inoculation, microbionation device is put in sterilization cassette, adopts high pressure to steamVapour sterilizing is also dry to reach germ-free condition, when inoculation, the suction nozzle of pipettor 67 is inserted in interface 1.(1) in the time need to exercising the function of oese, dip bacterium liquid or bacterium colony is inoculated with smooth round end 5,Groove 4 can be retained inoculation liquid, can increase qualitatively or reduce inoculum concentration, if want higher connecingThe amount of kind can lengthen the length of soft bar 3 immersed in liquid level of inoculation, if want to reduce inoculum concentration, can shortenInoculate the length of soft bar 3 immersed in liquid level; (2) in the time need to exercising the function of spreading rod, slightly firmly will be thinBacterium inoculator is oliquely downward pressed, and inoculates soft bar 3 and bends to Fig. 6 state at easy curved circle 2 places, and groove 4 can stayDeposit inoculation liquid, inoculation liquid can be transferred to other places, what in the time of coating, bacterium liquid distributed is more even.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all at thisWithin the spirit and principle of invention, any amendment of doing, be equal to replacement, improvement etc., all should be included inWithin protection scope of the present invention.
Claims (10)
1. a microbionation device, is characterized in that: comprise an interface and the soft bar of an inoculation, described inoculationThe top of soft bar is connected in the bottom of interface, and the bottom of the soft bar of described inoculation is provided with smooth round end, described in connectThe outer surface of planting soft bar is provided with groove, and described groove is along the axial setting of the soft bar of inoculation, the soft bar of described inoculationMiddle and upper part be provided with an easy curved circle, described easy curved circle is along the circumferential setting of the soft bar of inoculation, described easy curved circleDiameter is less than the diameter of inoculating soft bar.
2. a kind of microbionation device according to claim 1, is characterized in that: described groove is by connecingThe top of planting soft bar extends to smooth round end.
3. a kind of microbionation device according to claim 1, is characterized in that: described groove is evenBe distributed in the outer surface of the soft bar of inoculation.
4. a kind of microbionation device according to claim 3, is characterized in that: between adjacent notchesDistance equate.
5. a kind of microbionation device according to claim 1, is characterized in that: be positioned on easy curved circleThe diameter of the soft bar of inoculation of side reduces from top to bottom successively.
6. a kind of microbionation device according to claim 1, is characterized in that: described interface, connectThe material of planting soft bar and smooth round end is high-temperature resistance plastice.
7. a kind of microbionation device according to claim 6, is characterized in that: the material of described interfaceMatter is polypropylene, and the material of the soft bar of described inoculation and smooth round end is polytetrafluoroethylene (PTFE).
8. the method that the microbionation device as described in claim 1-7 any one carries out microbionation,It is characterized in that: (1) in the time need to exercising the function of oese, with smooth round end dip bacterium liquid orBacterium colony is inoculated, and groove can be retained inoculation liquid, can increase qualitatively or reduce inoculum concentration, according toInoculum concentration is selected the length of the soft bar immersed in liquid level of inoculation;
(2) in the time need to exercising the function of spreading rod, slightly firmly microbionation device is oliquely downward pressed,Inoculate soft bar in easy curved circle place's bending, groove can be retained inoculation liquid, inoculation liquid can be transferred to otherPlace, what in the time of coating, bacterium liquid distributed is more even.
9. a microbionation device, is characterized in that: comprise a pipettor and a microbionation device, instituteState microbionation device and comprise an interface and the soft bar of an inoculation, the top of the soft bar of described inoculation is connected in interfaceBottom, the bottom of the soft bar of described inoculation is provided with smooth round end, and the outer surface of the soft bar of described inoculation is provided with groove,Described groove is along the axial setting of the soft bar of inoculation, and the middle and upper part of the soft bar of described inoculation is provided with an easy curved circle, instituteState the circumferential setting of easy curved circle along the soft bar of inoculation, the diameter of described easy curved circle is less than the diameter of inoculating soft bar,Described pipettor and interface removably connect.
10. a kind of microbionation device according to claim 9, is characterized in that: described pipettorEnd be provided with a suction nozzle, described suction nozzle and interface removably connect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610165734.4A CN105586254B (en) | 2016-03-22 | 2016-03-22 | A kind of microbionation device and its method for carrying out microbionation |
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| CN201610165734.4A CN105586254B (en) | 2016-03-22 | 2016-03-22 | A kind of microbionation device and its method for carrying out microbionation |
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| CN105586254A true CN105586254A (en) | 2016-05-18 |
| CN105586254B CN105586254B (en) | 2017-09-12 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| IT202000008416A1 (en) * | 2020-04-20 | 2021-10-20 | Copan Italia Spa | Tool for dispensing a sample of biological or microbiological material and associated distribution process |
| WO2023077598A1 (en) * | 2021-11-02 | 2023-05-11 | 深圳先进技术研究院 | Apparatus and method for automated microorganism application |
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| WO2023077598A1 (en) * | 2021-11-02 | 2023-05-11 | 深圳先进技术研究院 | Apparatus and method for automated microorganism application |
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| CN105586254B (en) | 2017-09-12 |
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