CN105562710A - Method for biosynthesizing nano-silver through photoinduction of imperata leaf extract - Google Patents
Method for biosynthesizing nano-silver through photoinduction of imperata leaf extract Download PDFInfo
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- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 title claims abstract description 78
- 238000000034 method Methods 0.000 title claims abstract description 26
- 230000003570 biosynthesizing effect Effects 0.000 title claims abstract description 8
- 240000007171 Imperata cylindrica Species 0.000 title 1
- 241001598107 Imperata Species 0.000 claims abstract description 63
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000002245 particle Substances 0.000 claims abstract description 20
- 239000011159 matrix material Substances 0.000 claims abstract description 18
- 238000006243 chemical reaction Methods 0.000 claims abstract description 17
- 239000000843 powder Substances 0.000 claims abstract description 17
- 230000002194 synthesizing effect Effects 0.000 claims abstract description 13
- 239000006228 supernatant Substances 0.000 claims abstract description 10
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 3
- 239000012498 ultrapure water Substances 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims abstract 6
- 239000011259 mixed solution Substances 0.000 claims abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 20
- 239000011780 sodium chloride Substances 0.000 claims description 10
- 238000004108 freeze drying Methods 0.000 claims description 8
- 101710134784 Agnoprotein Proteins 0.000 claims description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- 238000000227 grinding Methods 0.000 claims description 2
- 238000000643 oven drying Methods 0.000 claims description 2
- 239000001103 potassium chloride Substances 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 244000166675 Cymbopogon nardus Species 0.000 claims 1
- 235000018791 Cymbopogon nardus Nutrition 0.000 claims 1
- 241000234643 Festuca arundinacea Species 0.000 claims 1
- 229920002994 synthetic fiber Polymers 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 description 22
- 238000003786 synthesis reaction Methods 0.000 description 15
- 239000008367 deionised water Substances 0.000 description 12
- 229910021641 deionized water Inorganic materials 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 238000002360 preparation method Methods 0.000 description 9
- 230000000844 anti-bacterial effect Effects 0.000 description 8
- 238000001035 drying Methods 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 7
- 238000004140 cleaning Methods 0.000 description 6
- 229910052709 silver Inorganic materials 0.000 description 6
- 239000004332 silver Substances 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 238000005303 weighing Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 238000012512 characterization method Methods 0.000 description 5
- 244000063299 Bacillus subtilis Species 0.000 description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 241000192125 Firmicutes Species 0.000 description 4
- 241000191967 Staphylococcus aureus Species 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000001308 synthesis method Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- -1 molecular detection Substances 0.000 description 2
- 239000002159 nanocrystal Substances 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 238000002371 ultraviolet--visible spectrum Methods 0.000 description 2
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 241000533770 Cayaponia Species 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000009713 electroplating Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000000608 laser ablation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000593 microemulsion method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 238000007738 vacuum evaporation Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
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- Condensed Matter Physics & Semiconductors (AREA)
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Abstract
The invention relates to the technical field of biology, in particular to a method for biosynthesizing nano-silver through photoinduction of imperata leaf extract. The method comprises the following steps: 1) picked imperata leaves are cleaned, dried and grinded to powder; ultrapure water is added in the powder for oscillating, extracting and centrifugating to obtain supernatant; and the supernatant is frozen and dried to obtain the imperata leaf extract; and 2) the imperata leaf extract in the step 1) is taken as a reaction matrix; AgNO3 solution is added, so that the concentration of the reaction matrix in obtained mixed solution is 10-50 mg/mL, and the concentration of AgNO3 is 1-3 mM; and nano-silver particles are prepared through reaction under sunlight irradiation or LED lamp irradiation. The biosynthesizing method is safe, environment-friendly, wide in synthetic material source and fast in synthesizing speed, can finish the reaction within 90-120 minutes, and is a green, safe and efficient biosynthesizing method.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a method for biosynthesizing nano silver by photoinduction of a cogongrass leaf extract.
Background
The nano silver is an atom or atom cluster with the particle size of 1-100nm, and the quantum mechanical effect on the nano scale endows the nano silver with various excellent physical, chemical and optical properties, so that the nano silver is widely applied to the fields of antibacterial materials, molecular detection, catalysts and the like (see the detailed reference 1: Charoupkak, MalamY, SeifialianAM. trends Biotechnol., 2010, 28: 580-588). The preparation of nano silver is different according to principles and can be divided into three major categories, namely a physical method, a chemical method and a biological synthesis method. The physical methods include vacuum evaporation, sputtering plating, laser ablation and the like, and the methods have high requirements on instruments and equipment and high production cost. The chemical methods include a microemulsion method, an electroplating method, an oxidation-reduction method, an electrochemical reduction method and the like, but the used chemical reagents are harmful to human bodies or environment to a certain extent, and some stabilizing agents or dispersing agents even have carcinogenicity. With the increase of environmental protection importance, it is necessary to find a green, safe and low-energy-consumption preparation method of nano silver. The starting materials used for the biosynthesis are microorganisms, enzymes and some natural plant materials (see in detail reference 2: NaikRR, StringerSJ, Agarwal G, JonesSE, StoneMO. nat. Mater., 2002, 1: 169-. Compared with microorganisms and enzymes, the method for synthesizing the nano-silver by the plants saves the link of cell culture, has relatively simple preparation process, and is more suitable for synthesizing the nano-silver on a large scale.
At present, it has been found that various plant substrates can mediate the synthesis of nanosilver, such as orange peel extract, red melon leaf extract, etc. (see in detail references 3: Kaviyas, Santhanalakshmij, Viswanathan B, MuthomurayJ, Srinivasan K. Spectrochim. acta, PartA, 2011, 79: 594-. However, due to the limitation of resources and regions, the screening of more novel and cheap plant substrates for the synthesis of nano-silver is of great significance. The cogongrass is a weed widely existing in nature, mostly grows on roadside, hillside and grassland, and is widely distributed in China. The invention discovers for the first time that the cogongrass leaf extract can efficiently biosynthesize nano silver through photoinduction, and has important application value.
Disclosure of Invention
The invention aims to provide a method for biosynthesizing nano silver by photoinduction of a cogongrass leaf extract.
The method for biosynthesizing nano silver by photoinduced cogongrass leaf extract according to the invention comprises the following steps:
1) washing the leaf of Imperata cylindrical for 3 times, oven drying at 60 deg.C, grinding into powder, adding ultrapure water 180r/min, oscillating for 2 hr, centrifuging at 10000rpm for 10min, and freeze drying the supernatant to obtain extract;
2) taking the extract of the leaves of the cogongrass in the step 1) as a reaction matrix, and adding AgNO3The solution of (1) is prepared so that the concentration of the extract of the leaf of the cogongrass is 10-50mg/mL, AgNO3The concentration is 1-3mM, and the reaction is carried out under the irradiation of sunlight or LED lamp to obtain the nano silver particles.
Wherein,
the method for synthesizing nano silver by photoinduced cogongrass leaf extract as claimed in claim 1, wherein AgNO is added in the step 2)3After the solution (2), Cl-containing solution is added dropwise-Solution of (2), making Cl in the mixed solution-Is in a concentration of 0.2-2 mM.
The method for synthesizing nano silver by photoinduced cogongrass leaf extract as claimed in claim 1, wherein the step 2) contains Cl-The solution of (a) comprises: NaCl, KCl or CaCl2。
The method for synthesizing nano silver by photoinduced cogongrass leaf extract according to claim 1, wherein the intensity of sunlight irradiation in the step 2) is as follows: 10000-100000lx, and the irradiation intensity of the LED lamp is 500-1000 lx.
According to an embodiment of the present invention, the method specifically includes the following steps:
a. preparation of biological matrices
Cleaning the picked imperata leaf with deionized water, placing the cleaned imperata leaf in a 60 ℃ oven for drying, and then preparing the dried imperata leaf into powder by a pulverizer for storage and standby.
Weighing 1g of imperata leaf powder, adding 20mL of deionized water, shaking at 37 ℃ and 200rpm for 10 min; centrifuging at 12000rpm for 20min, collecting supernatant, and freeze drying to obtain leaf extract of Imperata cylindrical as nanometer silver synthetic matrix.
b. Synthesis of nano silver
Adding AgNO to the above synthetic matrix3Stirring the solution for 10min at 200rpm of a magnetic stirrer, and then adding a NaCl solution to obtain a reaction system: the concentration of the matrix is 10-50mg/mL, AgNO3The final concentration is 1-3mM, the final concentration of NaCl is 0.2-2mM, and the reaction is carried out for 120min under the irradiation of sunlight or LED lamp, so as to obtain the nano silver particles.
c. Detection of nano-silver
The synthesis of the nano silver can be detected by an ultraviolet-visible spectrophotometry and color change; and (5) characterizing the particle size of the nano silver by a transmission electron microscope.
The method for synthesizing the nano-silver by photoinduction of the imperata leaf, provided by the invention, takes the imperata leaf as a reaction substrate, and is safe and reliable; the biological matrix prepared from the imperata leaf powder is used for synthesizing the nano silver particles, the reaction can be completed within 90-120min, and the synthesis speed is high; the synthesized nano silver is a nano crystal with the particle size distribution of 8-26 nm; the nano silver particle solution has good stability, and can be kept at room temperature for 2 months without obvious aggregation; the nano silver has higher antibacterial activity to gram-positive bacteria and gram-negative bacteria.
The method for synthesizing the nano-silver by the cogongrass leaf has stronger innovativeness and practicability and has the following advantages:
(1) the nano silver is synthesized by photoinduction of the imperata leaf for the first time, the synthesized nano silver is a nano crystal with the particle size distributed between 8 and 26nm, and the nano silver colloidal solution can keep high dispersion stability and has higher antibacterial activity on gram-positive bacteria and gram-negative bacteria.
(2) The synthesis method is safe, has no toxic or side effect, has high synthesis speed, can complete the reaction within 90-120min, and is an efficient, safe and environment-friendly synthesis method.
(3) The synthetic materials are safe, cheap and wide in source, mostly grow on roadsides, hillsides and grasslands, and are distributed all over the country.
Drawings
FIG. 1 is the UV-VIS absorption spectrum of synthesized nano-silver at the concentration of 10mg/mL of the extract from the leaf of Imperata cylindrical;
FIG. 2 is the UV-visible absorption spectrum of synthesized nano-silver at a silver nitrate concentration of 2 mM;
FIG. 3 is the UV-VIS absorption spectrum of nano-silver synthesized by adding 0.5mM NaCl;
FIG. 4 shows the statistics of the particle size distribution of the synthesized nano-silver from the extract of the leaves of Imperata cylindrical;
FIG. 5 shows the growth inhibition rate of nano-silver on liquid medium against Staphylococcus aureus, Bacillus subtilis and Escherichia coli.
Detailed Description
The present invention will be described in detail with reference to the following examples, but the present invention is not limited to these examples.
Example 1 biosynthesis of Nano silver particles by extract of leaf of Imperata cylindrical
a. Preparation of biological matrices
Cleaning the picked leaf blades of the cogongrass by deionized water, drying in a 60 ℃ drying oven, and then preparing the dried leaf blades of the cogongrass into powder by a pulverizer and storing for later use.
Weighing 1g of cogongrass rhizome powder, adding 20mL of deionized water, shaking at 37 ℃ and 200rpm for 10 min; centrifuging at 12000rpm for 20min, collecting supernatant, and freeze drying to obtain leaf extract of Imperata cylindrical as nanometer silver synthetic matrix.
b. Synthesis of nano silver
A1 mM solution of AgNO3 was added to a synthetic substrate having a concentration of 10mg/mL, and the reaction was carried out under LED lamp irradiation (1000lx) for 120 min.
c. Characterization of Nano-silver
The synthesis of nano silver can be characterized by color change or strong visible region absorption thereof, a reaction system without adding the biological matrix does not generate color change, and reddish brown is generated under the concentration of the biological matrix of 10 mg/mL. FIG. 1 shows: control samples without added imperata leaf extract, no visible absorption; the addition of 10mg/mL of the extract produced strong visible absorption, demonstrating that the extract from the leaf of Imperata cylindrical can mediate the synthesis of nano-silver.
Example 2 biosynthesis of Nano silver particles by extract of leaf of Imperata cylindrical
a. Preparation of biological matrices
Cleaning the picked leaf blades of the cogongrass by deionized water, drying in a 60 ℃ drying oven, and then preparing the dried leaf blades of the cogongrass into powder by a pulverizer and storing for later use.
Weighing 1g of cogongrass rhizome powder, adding 20mL of deionized water, shaking at 37 ℃ and 200rpm for 10 min; centrifuging at 12000rpm for 20min, collecting supernatant, and freeze drying to obtain leaf extract of Imperata cylindrical as nanometer silver synthetic matrix.
b. Synthesis of nano silver
A1 mM solution of AgNO3 was added to a synthetic substrate having a concentration of 50mg/mL, and the reaction was carried out under LED lamp irradiation (1000lx) for 120 min.
c. Characterization of Nano-silver
At a concentration of 50mg/mL of the biological matrix, the formationReddish brown and produces strong visible absorption, OD at 432nm432Reaching 4.87, the formation of nano silver is proved.
Example 3 biosynthesis of Nano silver particles by extract of leaf of Imperata cylindrical
a. Preparation of biological matrices
Cleaning the picked imperata leaf with deionized water, placing the cleaned imperata leaf in a 60 ℃ oven for drying, and then preparing the dried imperata leaf into powder by a pulverizer for storage and standby.
Weighing 1g of cogongrass rhizome powder, adding 20mL of deionized water, shaking at 37 ℃ and 200rpm for 10 min; centrifuging at 12000rpm for 20min, collecting supernatant, and freeze drying to obtain leaf extract of Imperata cylindrical as nanometer silver synthetic matrix.
b. Synthesis of nano silver
To a synthetic substrate having a concentration of 50mg/mL, a 2mM solution of AgNO3 was added and the reaction was carried out under sunlight (50000lx) for 120 min.
c. Characterization of Nano-silver
As shown in FIG. 2, no AgNO was added3No visible absorption of the control sample of solution; addition of 2mM AgNO3The solution generates strong visible region absorption, which proves the synthesis of the nano silver.
Example 4 biosynthesis of Nano silver particles by extract of leaf of Imperata cylindrical
a. Preparation of biological matrices
Cleaning the picked imperata leaf with deionized water, placing the cleaned imperata leaf in a 60 ℃ oven for drying, and then preparing the dried imperata leaf into powder by a pulverizer for storage and standby.
Weighing 1g of cogongrass rhizome powder, adding 20mL of deionized water, shaking at 37 ℃ and 200rpm for 10 min; centrifuging at 12000rpm for 20min, collecting supernatant, and freeze drying to obtain leaf extract of Imperata cylindrical as nanometer silver synthetic matrix.
b. Synthesis of nano silver
Adding 1mM AgNO to a synthetic matrix of concentration 50mg/mL3The solution was stirred magnetically, and then 0.5mM NaCl solution was added to the solution to react under LED lamp irradiation (1000lx) for 120 min.
c. Characterization of Nano-silver
As shown in fig. 3, the addition of 0.5mM NaCl increased the visible absorption compared to the control without NaCl, indicating that the addition of NaCl enhanced the synthesis of nanosilver.
Example 5 biosynthesis of Nano silver particles by extract of leaf of Imperata cylindrical
a. Preparation of biological matrices
Cleaning the picked imperata leaf with deionized water, placing the cleaned imperata leaf in a 60 ℃ oven for drying, and then preparing the dried imperata leaf into powder by a pulverizer for storage and standby.
Weighing 1g of cogongrass rhizome powder, adding 20mL of deionized water, shaking at 37 ℃ and 200rpm for 10 min; centrifuging at 12000rpm for 20min, collecting supernatant, and freeze drying to obtain leaf extract of Imperata cylindrical as nanometer silver synthetic matrix.
b. Synthesis of nano silver
Adding 1mM AgNO to a synthetic matrix of concentration 50mg/mL3The solution was stirred magnetically, and then 0.5mM NaCl solution was added to the solution to react under LED lamp irradiation (1000lx) for 120 min.
c. Characterization of Nano-silver
The particle size of the nano silver is counted by a transmission electron microscope, as shown in fig. 4, the particle size of the nano silver is distributed between 8 nm and 26nm, and the average particle size reaches 14.7 nm.
Evaluation of antibacterial Effect of Nano silver prepared in example 6
The invention investigates the antibacterial effect of the nano-silver on gram-positive bacteria (staphylococcus aureus, bacillus subtilis) and gram-negative bacteria (escherichia coli). Fig. 5 shows that the nano-silver particles synthesized in example 5 have significant antibacterial effects on staphylococcus aureus, bacillus subtilis and escherichia coli in a liquid culture medium, and the nano-silver concentration and the antibacterial effect show a certain dose-effect relationship. In addition, the nano silver prepared in examples 1 to 4 also has antibacterial effects against gram-positive bacteria (staphylococcus aureus, bacillus subtilis) and gram-negative bacteria (escherichia coli).
Claims (4)
1. A method for biosynthesizing nano silver by photoinduced cogongrass leaf extract, which is characterized by comprising the following steps:
1) washing the leaf of Imperata cylindrical for 3 times, oven drying at 60 deg.C, grinding into powder, adding ultrapure water 180r/min, oscillating for 2 hr, centrifuging at 10000rpm for 10min, and freeze drying the supernatant to obtain extract;
2) taking the extract of the leaves of the cogongrass in the step 1) as a reaction matrix, and adding AgNO3The solution of (1) is prepared so that the concentration of the Festuca arundinacea extract in the obtained mixed solution is 10-50mg/mL, AgNO3The concentration is 1-3mM, and the reaction is carried out under the irradiation of sunlight or LED lamp to obtain the nano silver particles.
2. The method for synthesizing nano silver by photoinduced citronella leaves as in claim 1, wherein AgNO is added in the step 2)3After the solution (2), Cl-containing solution is added dropwise-Solution of (2), making Cl in the mixed solution-Is in a concentration of 0.2-2 mM.
3. The method for synthesizing nano silver by photoinduced cogongrass leaf as claimed in claim 1, wherein in the step 2), Cl is contained-The solution of (a) comprises: NaCl, KCl or CaCl2。
4. The method for synthesizing nano silver by photoinduced cogongrass leaf according to claim 1, wherein the intensity of sunlight irradiation in the step 2) is as follows: 10000-100000lx, and the irradiation intensity of the LED lamp is 500-1000 lx.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106825607A (en) * | 2017-02-24 | 2017-06-13 | 华中农业大学 | A kind of method by photoinduction maize straw extract synthesizing nano-silver |
| CN106862590A (en) * | 2017-02-24 | 2017-06-20 | 华中农业大学 | A kind of method of utilization Guangxi Du chaste tree blade extract biosynthesis Nano Silver |
| CN108213457A (en) * | 2017-12-30 | 2018-06-29 | 吉林农业大学 | A kind of small size even Argent grain and preparation method thereof |
| CN109158613A (en) * | 2018-08-29 | 2019-01-08 | 扬州大学 | A method of utilizing sinigrin synthesizing nano-silver |
| CN109909512A (en) * | 2017-12-12 | 2019-06-21 | 中国科学院深圳先进技术研究院 | A kind of silver nanowires and its green magnanimity preparation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102888428A (en) * | 2011-07-21 | 2013-01-23 | 中国科学院过程工程研究所 | Method for synthesizing nano silver by utilizing Bacillus amyloliquefaciensBacillus amyloliquefaciens LSSE-62 |
| RU2477172C1 (en) * | 2011-11-10 | 2013-03-10 | Учреждение Российской академии наук Биолого-почвенный институт Дальневосточного отделения РАН | Method of producing metal nanoparticles |
| CN103785828A (en) * | 2014-02-25 | 2014-05-14 | 朱晓云 | Method for preparing silver-coated copper powder through light induction |
| IN2013MU01478A (en) * | 2013-04-22 | 2015-04-17 | Dnyandev Lokhande Prof Chandrakant | |
| IN2013MU02810A (en) * | 2013-08-28 | 2015-07-03 | Bharati Vidyapeeth Deemed University |
-
2016
- 2016-01-06 CN CN201610017660.XA patent/CN105562710A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102888428A (en) * | 2011-07-21 | 2013-01-23 | 中国科学院过程工程研究所 | Method for synthesizing nano silver by utilizing Bacillus amyloliquefaciensBacillus amyloliquefaciens LSSE-62 |
| RU2477172C1 (en) * | 2011-11-10 | 2013-03-10 | Учреждение Российской академии наук Биолого-почвенный институт Дальневосточного отделения РАН | Method of producing metal nanoparticles |
| IN2013MU01478A (en) * | 2013-04-22 | 2015-04-17 | Dnyandev Lokhande Prof Chandrakant | |
| IN2013MU02810A (en) * | 2013-08-28 | 2015-07-03 | Bharati Vidyapeeth Deemed University | |
| CN103785828A (en) * | 2014-02-25 | 2014-05-14 | 朱晓云 | Method for preparing silver-coated copper powder through light induction |
Non-Patent Citations (3)
| Title |
|---|
| KHAN BEHLOL AYAZ AHMED等: "β-Sitosterol-d-glucopyranoside isolated from Desmostachya bipinnata mediates photoinduced rapid green synthesis of silver nanoparticles", 《RSC ADVANCES》 * |
| 中国科学技术协会: "《颗粒学学科发展报告2009-2010》", 30 April 2010 * |
| 董丽红: "《两亲分子在纳米技术领域中的应用》", 31 October 2013 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106825607A (en) * | 2017-02-24 | 2017-06-13 | 华中农业大学 | A kind of method by photoinduction maize straw extract synthesizing nano-silver |
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| CN109909512A (en) * | 2017-12-12 | 2019-06-21 | 中国科学院深圳先进技术研究院 | A kind of silver nanowires and its green magnanimity preparation method |
| CN108213457A (en) * | 2017-12-30 | 2018-06-29 | 吉林农业大学 | A kind of small size even Argent grain and preparation method thereof |
| CN108213457B (en) * | 2017-12-30 | 2021-05-28 | 吉林农业大学 | Small-size uniform nano silver particles and preparation method thereof |
| CN109158613A (en) * | 2018-08-29 | 2019-01-08 | 扬州大学 | A method of utilizing sinigrin synthesizing nano-silver |
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