CN105560629A - Medicinal composition for treating postpartum abdominalgia with blood stasis, and preparation method thereof - Google Patents
Medicinal composition for treating postpartum abdominalgia with blood stasis, and preparation method thereof Download PDFInfo
- Publication number
- CN105560629A CN105560629A CN201511019566.XA CN201511019566A CN105560629A CN 105560629 A CN105560629 A CN 105560629A CN 201511019566 A CN201511019566 A CN 201511019566A CN 105560629 A CN105560629 A CN 105560629A
- Authority
- CN
- China
- Prior art keywords
- extract
- parts
- weight
- relative
- volumetric concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000008280 blood Substances 0.000 title claims abstract description 117
- 210000004369 blood Anatomy 0.000 title claims abstract description 116
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 239000000203 mixture Substances 0.000 title claims abstract description 15
- 239000000284 extract Substances 0.000 claims abstract description 130
- 239000011858 nanopowder Substances 0.000 claims abstract description 64
- 241000131329 Carabidae Species 0.000 claims abstract description 45
- 241000208368 Euonymus alatus Species 0.000 claims abstract description 16
- 241000301400 Trogopterus Species 0.000 claims abstract description 13
- 210000003608 fece Anatomy 0.000 claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 160
- 239000000706 filtrate Substances 0.000 claims description 55
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 52
- 239000000843 powder Substances 0.000 claims description 50
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 48
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 48
- 241001600153 Charybdis japonica Species 0.000 claims description 44
- 239000008194 pharmaceutical composition Substances 0.000 claims description 43
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 36
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 36
- 238000001556 precipitation Methods 0.000 claims description 36
- 238000010992 reflux Methods 0.000 claims description 36
- 239000006228 supernatant Substances 0.000 claims description 36
- 239000007788 liquid Substances 0.000 claims description 31
- 238000000034 method Methods 0.000 claims description 30
- 238000009835 boiling Methods 0.000 claims description 24
- 238000001035 drying Methods 0.000 claims description 24
- 238000000605 extraction Methods 0.000 claims description 24
- 239000012530 fluid Substances 0.000 claims description 24
- 239000002245 particle Substances 0.000 claims description 24
- YKRGDOXKVOZESV-WRJNSLSBSA-N Paeoniflorin Chemical group C([C@]12[C@H]3O[C@]4(O)C[C@](O3)([C@]1(C[C@@H]42)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)C)OC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-WRJNSLSBSA-N 0.000 claims description 18
- 229930013930 alkaloid Natural products 0.000 claims description 18
- 240000000073 Achillea millefolium Species 0.000 claims description 15
- 235000007754 Achillea millefolium Nutrition 0.000 claims description 15
- 240000009087 Crescentia cujete Species 0.000 claims description 15
- 235000005983 Crescentia cujete Nutrition 0.000 claims description 15
- 240000003537 Ficus benghalensis Species 0.000 claims description 15
- 235000009797 Lagenaria vulgaris Nutrition 0.000 claims description 15
- 239000010271 massa medicata fermentata Substances 0.000 claims description 15
- 241000722118 Thlaspi Species 0.000 claims description 14
- 239000012567 medical material Substances 0.000 claims description 14
- 229910052799 carbon Inorganic materials 0.000 claims description 13
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 12
- 206010053615 Thermal burn Diseases 0.000 claims description 12
- 239000002253 acid Substances 0.000 claims description 12
- 230000001476 alcoholic effect Effects 0.000 claims description 12
- 238000004458 analytical method Methods 0.000 claims description 12
- 239000003729 cation exchange resin Substances 0.000 claims description 12
- 238000004140 cleaning Methods 0.000 claims description 12
- 239000012141 concentrate Substances 0.000 claims description 12
- 239000008367 deionised water Substances 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 12
- 239000008187 granular material Substances 0.000 claims description 12
- 238000003306 harvesting Methods 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 12
- 238000004321 preservation Methods 0.000 claims description 12
- 239000000047 product Substances 0.000 claims description 12
- 238000010298 pulverizing process Methods 0.000 claims description 12
- 238000010926 purge Methods 0.000 claims description 12
- 238000010898 silica gel chromatography Methods 0.000 claims description 12
- 238000005406 washing Methods 0.000 claims description 12
- 239000007921 spray Substances 0.000 claims description 11
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 7
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 5
- 229920000881 Modified starch Polymers 0.000 claims description 5
- 238000005469 granulation Methods 0.000 claims description 5
- 230000003179 granulation Effects 0.000 claims description 5
- 235000019359 magnesium stearate Nutrition 0.000 claims description 5
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 5
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 5
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 208000002193 Pain Diseases 0.000 abstract description 15
- 230000036407 pain Effects 0.000 abstract description 15
- 230000017531 blood circulation Effects 0.000 abstract description 13
- 239000003814 drug Substances 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 9
- 206010019233 Headaches Diseases 0.000 abstract description 2
- 231100000869 headache Toxicity 0.000 abstract description 2
- 241000132059 Adenocaulon himalaicum Species 0.000 abstract 1
- 240000003690 Callicarpa japonica Species 0.000 abstract 1
- 235000017595 Callicarpa japonica Nutrition 0.000 abstract 1
- 244000173782 Ficus hirta Species 0.000 abstract 1
- 240000002373 Ficus pumila Species 0.000 abstract 1
- 235000006718 Ficus pumila Nutrition 0.000 abstract 1
- 241001558017 Gynura Species 0.000 abstract 1
- 241000561705 Kadsura coccinea Species 0.000 abstract 1
- 241000634758 Leonurus pseudomacranthus Species 0.000 abstract 1
- 244000086741 Lindernia ciliata Species 0.000 abstract 1
- 241000522652 Ormosia <angiosperm> Species 0.000 abstract 1
- 206010033557 Palpitations Diseases 0.000 abstract 1
- 208000018525 Postpartum Hemorrhage Diseases 0.000 abstract 1
- 240000002044 Rhizophora apiculata Species 0.000 abstract 1
- 240000000760 Viola betonicifolia Species 0.000 abstract 1
- 230000001914 calming effect Effects 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 230000002936 tranquilizing effect Effects 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
- 239000002023 wood Substances 0.000 abstract 1
- 208000004998 Abdominal Pain Diseases 0.000 description 15
- 230000001737 promoting effect Effects 0.000 description 14
- 230000008961 swelling Effects 0.000 description 11
- 210000004291 uterus Anatomy 0.000 description 11
- 201000000736 Amenorrhea Diseases 0.000 description 8
- 206010001928 Amenorrhoea Diseases 0.000 description 8
- 231100000540 amenorrhea Toxicity 0.000 description 8
- 208000014674 injury Diseases 0.000 description 8
- 208000024891 symptom Diseases 0.000 description 8
- 206010000087 Abdominal pain upper Diseases 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 210000000952 spleen Anatomy 0.000 description 7
- 230000008736 traumatic injury Effects 0.000 description 7
- 206010007247 Carbuncle Diseases 0.000 description 6
- 208000027418 Wounds and injury Diseases 0.000 description 6
- 230000007812 deficiency Effects 0.000 description 6
- 231100000614 poison Toxicity 0.000 description 6
- 239000003440 toxic substance Substances 0.000 description 6
- 208000005171 Dysmenorrhea Diseases 0.000 description 5
- 206010013935 Dysmenorrhoea Diseases 0.000 description 5
- 206010027514 Metrorrhagia Diseases 0.000 description 5
- 206010030113 Oedema Diseases 0.000 description 5
- 206010052428 Wound Diseases 0.000 description 5
- 208000019255 Menstrual disease Diseases 0.000 description 4
- 210000001015 abdomen Anatomy 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 4
- 210000003734 kidney Anatomy 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 238000001694 spray drying Methods 0.000 description 4
- 230000001225 therapeutic effect Effects 0.000 description 4
- 206010051625 Conjunctival hyperaemia Diseases 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000023597 hemostasis Effects 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 208000037920 primary disease Diseases 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 239000011800 void material Substances 0.000 description 3
- 206010000084 Abdominal pain lower Diseases 0.000 description 2
- 206010003011 Appendicitis Diseases 0.000 description 2
- 206010010726 Conjunctival oedema Diseases 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 2
- 208000036119 Frailty Diseases 0.000 description 2
- 206010017553 Furuncle Diseases 0.000 description 2
- 208000032843 Hemorrhage Diseases 0.000 description 2
- 208000006877 Insect Bites and Stings Diseases 0.000 description 2
- 206010023126 Jaundice Diseases 0.000 description 2
- 208000004880 Polyuria Diseases 0.000 description 2
- 208000025747 Rheumatic disease Diseases 0.000 description 2
- 208000036029 Uterine contractions during pregnancy Diseases 0.000 description 2
- 206010046910 Vaginal haemorrhage Diseases 0.000 description 2
- 230000003187 abdominal effect Effects 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 206010003549 asthenia Diseases 0.000 description 2
- 208000006673 asthma Diseases 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000035606 childbirth Effects 0.000 description 2
- 230000004087 circulation Effects 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000035619 diuresis Effects 0.000 description 2
- 208000001848 dysentery Diseases 0.000 description 2
- 201000006549 dyspepsia Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 208000035861 hematochezia Diseases 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 230000002175 menstrual effect Effects 0.000 description 2
- 230000005906 menstruation Effects 0.000 description 2
- 210000002826 placenta Anatomy 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 238000004080 punching Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000000552 rheumatic effect Effects 0.000 description 2
- 206010040872 skin infection Diseases 0.000 description 2
- 239000002435 venom Substances 0.000 description 2
- 210000001048 venom Anatomy 0.000 description 2
- 231100000611 venom Toxicity 0.000 description 2
- 206010000077 Abdominal mass Diseases 0.000 description 1
- 208000010470 Ageusia Diseases 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 208000007882 Gastritis Diseases 0.000 description 1
- 208000005577 Gastroenteritis Diseases 0.000 description 1
- 208000012671 Gastrointestinal haemorrhages Diseases 0.000 description 1
- 208000034507 Haematemesis Diseases 0.000 description 1
- 206010019909 Hernia Diseases 0.000 description 1
- 241000218231 Moraceae Species 0.000 description 1
- 206010050031 Muscle strain Diseases 0.000 description 1
- 206010050819 Musculoskeletal chest pain Diseases 0.000 description 1
- 241000475481 Nebula Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 240000005373 Panax quinquefolius Species 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 206010067268 Post procedural infection Diseases 0.000 description 1
- 208000029785 Puerperal disease Diseases 0.000 description 1
- 208000004078 Snake Bites Diseases 0.000 description 1
- 206010042674 Swelling Diseases 0.000 description 1
- 206010053476 Traumatic haemorrhage Diseases 0.000 description 1
- 206010046788 Uterine haemorrhage Diseases 0.000 description 1
- 206010046798 Uterine leiomyoma Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 206010048038 Wound infection Diseases 0.000 description 1
- 208000019790 abdominal distention Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 231100000176 abortion Toxicity 0.000 description 1
- 208000012873 acute gastroenteritis Diseases 0.000 description 1
- 230000036592 analgesia Effects 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 210000000038 chest Anatomy 0.000 description 1
- 208000023652 chronic gastritis Diseases 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000002934 diuretic Substances 0.000 description 1
- 230000001882 diuretic effect Effects 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 208000001780 epistaxis Diseases 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 210000002219 extraembryonic membrane Anatomy 0.000 description 1
- 208000024756 faint Diseases 0.000 description 1
- 208000006750 hematuria Diseases 0.000 description 1
- 230000002607 hemopoietic effect Effects 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 210000003026 hypopharynx Anatomy 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 201000003453 lung abscess Diseases 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 208000004396 mastitis Diseases 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 210000004914 menses Anatomy 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 201000008383 nephritis Diseases 0.000 description 1
- 201000005737 orchitis Diseases 0.000 description 1
- YKRGDOXKVOZESV-UHFFFAOYSA-N paeoniflorin Natural products O1C(C)(C2(CC34)OC5C(C(O)C(O)C(CO)O5)O)CC3(O)OC1C24COC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-UHFFFAOYSA-N 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 210000001034 respiratory center Anatomy 0.000 description 1
- 201000003068 rheumatic fever Diseases 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 206010042772 syncope Diseases 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/86—Violaceae (Violet family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/24—Mucus; Mucous glands; Bursa; Synovial fluid; Arthral fluid; Excreta; Spinal fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/612—Crustaceans, e.g. crabs, lobsters, shrimps, krill or crayfish; Barnacles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/37—Celastraceae (Staff-tree or Bittersweet family), e.g. tripterygium or spindletree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/533—Leonurus (motherwort)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/79—Schisandraceae (Schisandra family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
- A61K9/2059—Starch, including chemically or physically modified derivatives; Amylose; Amylopectin; Dextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Insects & Arthropods (AREA)
- Biomedical Technology (AREA)
- Neurology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Zoology (AREA)
- Marine Sciences & Fisheries (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a medicinal composition for treating postpartum abdominalgia with blood stasis, and a preparation method thereof. The medicinal composition for treating postpartum abdominalgia with blood stasis comprises creeping fig root, Adenocaulon himalaicum Edgew., Kadsura coccinea, purple gynura root, Viola betonicifolia J. E. Smith, wood of Henry ormosia, Boor's Mustard Herb, Euonymus alatus (Thunb.)Sieb., Leonurus pseudomacranthus Kitagawa, Herba Lycopi, root and inflorescence receptacle of Ficus simplicissima Lour. var. hirta (Vahl) Migo, root or leaf of Bigleaf Beautyberry, herb of Lindernia ciliata (Colsm.) Pennell, medicated leaven, Herba Leonuri extract, radix paeoniae rubra extract, trogopterus dung extract, female ground beetle nano-powder, and mangrove crab nano-powder. The medicinal composition is elaborately prepared by combining clinic experiences through reasonably selecting medicines according to a traditional Chinese medicine dialectical theory, has blood circulation invigorating, channel freeing, blood stasis transforming, pain relieving, blood breaking, qi moving, calming and mind tranquillizing effects, has substantial treatment effects on postpartum abdominalgia with blood stasis, postpartum hemorrhage, postpartum palpitation and postpartum headaches, is mild in property, and is safe to puerperae and babies lactated by the puerperal without side effects.
Description
Technical field
The present invention relates to medical art, specifically relate to and a kind ofly treat pharmaceutical composition of abdominalgia with blood stasis in puerperal and preparation method thereof.
Background technology
Puerperal, abdominalgia with blood stasis referred to metrorrhagia in puerperal, uterus meromorphosis, prolonged lochia, and after artificial abortion, vaginal hemorrhage etc. cause the symptom of women's lower abdominal pain.The traditional Chinese medical science is thought, primary disease pathology mainly QI-blood circulation is not normal, stagnation of QI and blood.Face in disease and think that primary disease is the card of deficiency in origin and excess in superficiality in fact.Deficiency in origin is spleen and suffers from a deficiency of the kidney, and mark is real is blood stasis.The pregnant foster born of the same parents' tire of married woman, need consume cloudy blood; During childbirth firmly, perspire, maternal infuries is lost blood and postpartum breastfeeding increases the weight of again the loss of QI and blood; Deficiency of qi and blood, the debility of CHONG and REN meridians, this had both been the special physiological state in married woman's puerperal, was also the cause of disease of the many void of puerperal disease, died people's all days " all joints in puerpera being hollow and deficient ".If puerpera's kidney qi foot, essence can activating QI hemopoietic, and then QI and blood biochemistry is inexhaustible for spleen health movement, and QI and blood is gradually prosperous, and the sea of blood gradually fills, and logical Sheng of conception vessel and uterus are from flourish, and blood is taken the photograph in contracting palace and lochia can be exhausted.If married woman's weakness of the spleen and stomach or vigour element void usually, hinder in gestation, childbirth again, gas damages blood and consumes the void of then spleen kidney more very.Dysfunction of the spleen in transportation then QI and blood is biochemical deficient, has under one's command and haves no right; Suffer from a deficiency of the kidney, the angry blood of being difficult of damage of essence insufficiency of blood moistens supports punching and appoints, and decline of the fire from the gate of life, can not warm uterus; The QI and blood that puerpera consumes wound can not be taken a tonic or nourishing food to build up one's health in time, the debility of CHONG and REN meridians and cause lochia non-stop.Though the sick position of primary disease is appointed in punching, uterus, change in QI and blood, be originally deficiency of spleen and stomach, mark is real is blood stasis.Doctor trained in Western medicine is thought, its cause of disease mainly uterus meromorphosis, and when part Placenta Hominis, fetal membrane is residual or infect and affect uterine contraction and recovery; Or puerpera as suffered from chronic disease, severe loss of blood, excessively tired, body constitution fails to recover, or the local factor such as uterus excessive expansion, hysteromyoma, all can affect uterus meromorphosis.Secondly, cesarean postoperate, when Uterus wall disruption of wound, operation, hemostasis is thoroughly or because of postoperative infection, affect uterine involution and causes bleeding incessantly.
At present women uterus in puerperal abdominalgia with blood stasis is mainly treated by Chinese medicine and Western medicine two kinds of methods.Doctor trained in Western medicine generally adopts accelerates uterine contraction with hemostasis, and enrich blood, continue with antibiotic infection control etc., though through treatment energy relief of symptoms, have certain untoward reaction, therapeutic effect is not satisfactory, although the effect of getting instant result can be reached when treating this disease, but during the abdominalgia with blood stasis disease of western medicine women uterus in puerperal, side effect is larger, easy recurrent exerbation, and fundamentally can not thoroughly cure this disease.China's traditional medicine utilizes treatment by Chinese herbs women uterus in puerperal abdominalgia with blood stasis to have a long history, Chinese medicine not only can reach the effect of invigorating blood circulation rapidly, the more important thing is and can also carry out double recuperation to human body, thus reach equilibrium between yin and yang, QI and blood is normally run, reaches the object of hemostasis.
Prior art prescriptions of Chinese medicine lacks dialectical, and treatment cycle is long, and suiting the medicine to the illness property is not strong, and curative effect is not obvious, even also can bring side effect to patient, increases patient suffering.
Summary of the invention
The technical problem that the present invention solves is to provide a kind ofly treats pharmaceutical composition of abdominalgia with blood stasis in puerperal and preparation method thereof.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is as follows:
The pharmaceutical composition being used for the treatment of postnatal blood stasis stomachache is grouped into by the one-tenth of following weight portion: Radix Fici pumilae 89 ~ 103 parts, 76 ~ 90 parts, calabash leaf, Caulis Kadsurae Coccineae 73 ~ 87 parts, Radix Gynurae bicoloris 68 ~ 84 parts, Herba Violae Inconspicui 59 ~ 77 parts, lignum Ormosiae henryi 47 ~ 65 parts, Herba Thlaspis 40 ~ 54 parts, Xinjiang winged euonymus 37 ~ 45 parts, Herba leonuri pseudoma cranthi 31 ~ 39 parts, Herba Lycopi 25 ~ 33 parts, palm leaf banyan 18 ~ 26 parts, Radix Callicarpae Bodinieri 13 ~ 19 parts, alpine yarrow 7 ~ 15 parts, Massa Medicata Fermentata 4 ~ 6 parts, Herba Leonuri extract 5 ~ 8 parts, Radix Paeoniae Rubra extract 2 ~ 4 parts, Oletum Trogopterori extract 1 ~ 3 part, female ground beetle nano powder 6 ~ 9 parts, Charybdis japonica nano powder 4 ~ 7 parts.
Further, described a kind of pharmaceutical composition for the treatment of postnatal blood stasis stomachache is grouped into by the one-tenth of following weight portion: Radix Fici pumilae 96 parts, 84 parts, calabash leaf, Caulis Kadsurae Coccineae 80 parts, Radix Gynurae bicoloris 76 parts, Herba Violae Inconspicui 67 parts, lignum Ormosiae henryi 56 parts, Herba Thlaspis 47 parts, Xinjiang winged euonymus 41 parts, Herba leonuri pseudoma cranthi 35 parts, Herba Lycopi 29 parts, palm leaf banyan 22 parts, Radix Callicarpae Bodinieri 16 parts, alpine yarrow 11 parts, Massa Medicata Fermentata 5 parts, Herba Leonuri extract 6.5 parts, Radix Paeoniae Rubra extract 3 parts, Oletum Trogopterori extract 2 parts, female ground beetle nano powder 7.5 parts, Charybdis japonica nano powder 5.5 parts.
Further, described Herba Leonuri extract is Total alkaloids, its extracting method is: the Herba Leonuri getting fresh harvesting, add the water boiling and extraction 2 ~ 3 times relative to Herba Leonuri 5 times of weight, each extraction 1 ~ 2 hour, merge extractive liquid, then concentrated extracting solution is to water content 35%, then adding relative to the volumetric concentration of concentrated solution 3 times of weight in concentrated solution is the alcoholic solution of 95%, cold preservation 2 hours at 4 ~ 6 DEG C, then ethanol is reclaimed, add wherein volumetric concentration be 3% hydrochloric acid regulate PH to 2 ~ 3, filter, getting filtrate is added on acid cation exchange resin post, first use washed with de-ionized water, be 3% hydrochloric acid cleaning by volumetric concentration again, merge all cleanout fluid, and in cleanout fluid, add the sodium hydroxide solution adjustment PH to 7 that mass concentration is 40%, leave standstill 10min to filter, concentrate filtrate to thick paste, then at 70 ~ 80 DEG C by thick paste drying under reduced pressure, obtain Herba Leonuri extract Total alkaloids, Total alkaloids has direct excitation to respiratory center, and there is activating blood circulation to dissipate blood stasis, the effect of inducing diuresis to remove edema.
Further, described Radix Paeoniae Rubra extract is peoniflorin, and its extracting method is: get Radix Paeoniae Rubra dry product pulverize after cross 100 mesh sieves, then add relative to the volumetric concentration of Radix Paeoniae Rubra powder 3 times of weight be 60% aqueous acetone solution reflux, extract, 3h, filter, filtrate is for subsequent use; Filtering residue uses the aqueous acetone solution reflux, extract, twice of 60% again, extracting solution and above-mentioned filtrate are merged, centrifugal, then by the supernatant silica gel column chromatography of centrifugal gained, gained chromatographic solution is evaporated to the extractum that relative density is 1.05 ~ 1.25 at 45 ~ 75 DEG C, extractum is dried and is pulverized to constant weight, and obtain Radix Paeoniae Rubra extract peoniflorin, paeoniflorin has significant analgesia, calmness, convulsion, antithrombotic effect.
Further, the extracting method of described Oletum Trogopterori extract is: be placed in by Oletum Trogopterori in dehydrator, dry to moisture lower than 1% for 60 ~ 70 DEG C, taking-up is cooled to room temperature, be ground into coarse powder, be the alcohol heating reflux 2 times of 95% successively by the volumetric concentration relative to Trogopterus Dung 7 times of weight, be respectively 2 hours, 1 hour at every turn, merge extractive liquid, is evaporated to extractum; Add relative to the volumetric concentration of extractum 6 times of weight be 95% dissolve with ethanol extractum, filter, obtain supernatant, then with active carbon particle filter, be the ethanol purge activated carbon granule of 95% by the volumetric concentration relative to supernatant 3 times of weight.Collect ethanol rinse, being concentrated into determining alcohol is 40%, at 24 DEG C, place cold analysis in 8 hours, filters to obtain precipitation, is the washing with alcohol precipitation of 60% by the volumetric concentration relative to precipitation 4 times of weight, drying precipitated, obtains Oletum Trogopterori extract.
The preparation method of further Suo Shu female ground beetle nano powder and Charybdis japonica nano powder is: get Huo female ground beetle and Charybdis japonica respectively, scald dead with boiling water and clean up, then be placed in 50 ~ 70 DEG C, marmite inherence low temperature and bake 2 ~ 3 hours, taking-up is cooled to room temperature, first be ground into super micron mill the fine powder that particle diameter is 120 ~ 150 μm, and then obtain 1 ~ 100nm female ground beetle nano powder and Charybdis japonica nano powder respectively after nanoscale pulverization process.
Further, the preparation method of the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal, comprises the following steps:
(1) Radix Fici pumilae is taken by proportioning, calabash leaf, Caulis Kadsurae Coccineae, Radix Gynurae bicoloris, Herba Violae Inconspicui, lignum Ormosiae henryi, Herba Thlaspis, Xinjiang winged euonymus, Herba leonuri pseudoma cranthi, Herba Lycopi, palm leaf banyan, Radix Callicarpae Bodinieri, alpine yarrow, Massa Medicata Fermentata, add the water relative to medical material 10 times of weight, soak 3 ~ 5h, decoct 2 ~ 3 hours, filter, medicinal residues add the water of medical material 8 times of weight again, decoct 1 ~ 2 hour, medicinal liquid leaches, merge twice decoction liquor, being evaporated to 60 DEG C of relative densities is the extractum of 1.05, adding relative to the volumetric concentration of extractum 3 times of weight is the ethanol of 75%, stir, leave standstill, filter, during filtrate reduced in volume to 65 DEG C, relative density is the concentrated solution of 1.05 ~ 1.25, and reclaim ethanol, concentrated solution is spray dried to extract powder, cross 100 mesh sieves, for subsequent use, spray drying condition is: inlet temperature is 100 DEG C, and leaving air temp is 90 DEG C, and temperature of charge is 70 DEG C, and atomizing pressure is 0.2 MPa, and spray velocity is 10ml/s,
(2) Herba Leonuri of fresh harvesting is got, add the water boiling and extraction 2 ~ 3 times relative to Herba Leonuri 5 times of weight, each extraction 1 ~ 2 hour, merge extractive liquid, then concentrated extracting solution is to water content 35%, then adding relative to the volumetric concentration of concentrated solution 3 times of weight in concentrated solution is the alcoholic solution of 95%, cold preservation 2 hours at 4 ~ 6 DEG C, then ethanol is reclaimed, add wherein volumetric concentration be 3% hydrochloric acid regulate PH to 2 ~ 3, filter, getting filtrate is added on acid cation exchange resin post, first use washed with de-ionized water, be 3% hydrochloric acid cleaning by volumetric concentration again, merge all cleanout fluid, and in cleanout fluid, add the sodium hydroxide solution adjustment PH to 7 that mass concentration is 40%, leave standstill 10min to filter, concentrate filtrate to thick paste, then at 70 ~ 80 DEG C by thick paste drying under reduced pressure, obtain Herba Leonuri extract Total alkaloids, for subsequent use,
(3) get Radix Paeoniae Rubra dry product pulverize after cross 100 mesh sieves, then add relative to the volumetric concentration of Radix Paeoniae Rubra powder 3 times of weight be 60% aqueous acetone solution reflux, extract, 3h, filter, filtrate is for subsequent use; Filtering residue uses the aqueous acetone solution reflux, extract, twice of 60% again, extracting solution and above-mentioned filtrate are merged, centrifugal, then by the supernatant silica gel column chromatography of centrifugal gained, gained chromatographic solution is evaporated to the extractum that relative density is 1.05 ~ 1.25 at 45 ~ 75 DEG C, extractum is dried and is pulverized to constant weight, obtains Radix Paeoniae Rubra extract peoniflorin, for subsequent use;
(4) Oletum Trogopterori is placed in dehydrator, dry to moisture lower than 1% for 60 ~ 70 DEG C, taking-up is cooled to room temperature, be ground into coarse powder, be the alcohol heating reflux 2 times of 95% successively by the volumetric concentration relative to Trogopterus Dung 7 times of weight, be respectively 2 hours, 1 hour, merge extractive liquid, is evaporated to extractum at every turn; Add relative to the volumetric concentration of extractum 6 times of weight be 95% dissolve with ethanol extractum, filter, obtain supernatant, then with active carbon particle filter, be the ethanol purge activated carbon granule of 95% by the volumetric concentration relative to supernatant 3 times of weight.Collect ethanol rinse, being concentrated into determining alcohol is 40%, at 24 DEG C, place cold analysis in 8 hours, filters to obtain precipitation, is the washing with alcohol precipitation of 60% by the volumetric concentration relative to precipitation 4 times of weight, drying precipitated, obtains Oletum Trogopterori extract, for subsequent use;
(5) Huo female ground beetle and Charybdis japonica is got respectively, scald dead with boiling water and clean up, then be placed in 50 ~ 70 DEG C, marmite inherence low temperature and bake 2 ~ 3 hours, taking-up is cooled to room temperature, first be ground into super micron mill the fine powder that particle diameter is 120 ~ 150 μm, and then 1 ~ 100nm female ground beetle nano powder and Charybdis japonica nano powder is obtained respectively after nanoscale pulverization process, for subsequent use;
(6) Herba Leonuri extract, Radix Paeoniae Rubra extract, Oletum Trogopterori extract, female ground beetle nano powder and Charybdis japonica nano powder is taken by proportioning, mix homogeneously in dispensing canister with the extract powder of step (1) gained, the pharmaceutical composition of abdominalgia with blood stasis in puerperal must be treated;
Further, tablet is made by the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal.
Further, the preparation method of the medicinal composition tablets of described treatment abdominalgia with blood stasis in puerperal, comprises the following steps:
(1) Radix Fici pumilae is taken by proportioning, calabash leaf, Caulis Kadsurae Coccineae, Radix Gynurae bicoloris, Herba Violae Inconspicui, lignum Ormosiae henryi, Herba Thlaspis, Xinjiang winged euonymus, Herba leonuri pseudoma cranthi, Herba Lycopi, palm leaf banyan, Radix Callicarpae Bodinieri, alpine yarrow, Massa Medicata Fermentata, add the water relative to medical material 10 times of weight, soak 3 ~ 5h, decoct 2 ~ 3 hours, filter, medicinal residues add the water of medical material 8 times of weight again, decoct 1 ~ 2 hour, medicinal liquid leaches, merge twice decoction liquor, being evaporated to 60 DEG C of relative densities is the extractum of 1.05, adding relative to the volumetric concentration of extractum 3 times of weight is the ethanol of 75%, stir, leave standstill, filter, during filtrate reduced in volume to 65 DEG C, relative density is the concentrated solution of 1.05 ~ 1.25, and reclaim ethanol, concentrated solution is spray dried to extract powder, cross 100 mesh sieves, for subsequent use,
(2) Herba Leonuri of fresh harvesting is got, add the water boiling and extraction 2 ~ 3 times relative to Herba Leonuri 5 times of weight, each extraction 1 ~ 2 hour, merge extractive liquid, then concentrated extracting solution is to water content 35%, then adding relative to the volumetric concentration of concentrated solution 3 times of weight in concentrated solution is the alcoholic solution of 95%, cold preservation 2 hours at 4 ~ 6 DEG C, then ethanol is reclaimed, add wherein volumetric concentration be 3% hydrochloric acid regulate PH to 2 ~ 3, filter, getting filtrate is added on acid cation exchange resin post, first use washed with de-ionized water, be 3% hydrochloric acid cleaning by volumetric concentration again, merge all cleanout fluid, and in cleanout fluid, add the sodium hydroxide solution adjustment PH to 7 that mass concentration is 40%, leave standstill 10min to filter, concentrate filtrate to thick paste, then at 70 ~ 80 DEG C by thick paste drying under reduced pressure, obtain Herba Leonuri extract Total alkaloids, for subsequent use,
(3) get Radix Paeoniae Rubra dry product pulverize after cross 100 mesh sieves, then add relative to the volumetric concentration of Radix Paeoniae Rubra powder 3 times of weight be 60% aqueous acetone solution reflux, extract, 3h, filter, filtrate is for subsequent use; Filtering residue uses the aqueous acetone solution reflux, extract, twice of 60% again, extracting solution and above-mentioned filtrate are merged, centrifugal, then by the supernatant silica gel column chromatography of centrifugal gained, gained chromatographic solution is evaporated to the extractum that relative density is 1.05 ~ 1.25 at 45 ~ 75 DEG C, extractum is dried and is pulverized to constant weight, obtains Radix Paeoniae Rubra extract peoniflorin, for subsequent use;
(4) Oletum Trogopterori is placed in dehydrator, dry to moisture lower than 1% for 60 ~ 70 DEG C, taking-up is cooled to room temperature, be ground into coarse powder, be the alcohol heating reflux 2 times of 95% successively by the volumetric concentration relative to Trogopterus Dung 7 times of weight, be respectively 2 hours, 1 hour, merge extractive liquid, is evaporated to extractum at every turn; Add relative to the volumetric concentration of extractum 6 times of weight be 95% dissolve with ethanol extractum, filter, obtain supernatant, then with active carbon particle filter, be the ethanol purge activated carbon granule of 95% by the volumetric concentration relative to supernatant 3 times of weight.Collect ethanol rinse, being concentrated into determining alcohol is 40%, at 24 DEG C, place cold analysis in 8 hours, filters to obtain precipitation, is the washing with alcohol precipitation of 60% by the volumetric concentration relative to precipitation 4 times of weight, drying precipitated, obtains Oletum Trogopterori extract, for subsequent use;
(5) Huo female ground beetle and Charybdis japonica is got respectively, scald dead with boiling water and clean up, then be placed in 50 ~ 70 DEG C, marmite inherence low temperature and bake 2 ~ 3 hours, taking-up is cooled to room temperature, first be ground into super micron mill the fine powder that particle diameter is 120 ~ 150 μm, and then 1 ~ 100nm female ground beetle nano powder and Charybdis japonica nano powder is obtained respectively after nanoscale pulverization process, for subsequent use;
(6) Herba Leonuri extract, Radix Paeoniae Rubra extract, Oletum Trogopterori extract, female ground beetle nano powder and Charybdis japonica nano powder is taken by proportioning, mix homogeneously in dispensing canister with the extract powder of step (1) gained, the pharmaceutical composition of abdominalgia with blood stasis in puerperal must be treated;
(7) in the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal, 12 ~ 20% pregelatinized Starch, 3 ~ 5% microcrystalline Cellulose, 0.1 ~ 0.2% BHA, 0.2 ~ 0.3% magnesium stearate is added, mixing, granulation, tabletting, obtain the tablet of the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal.
Wherein: Radix Fici pumilae: bitter in the mouth, property is put down; Expelling wind and removing dampness, channels sootheing and network vessel quickening; Control headache dizzy, articular rheumatic pain, puerperal exposure to wind; For the root of moraceae plants Caulis fici pumilae (Fructus Fici Pumilae), can adopt at any time, dry for subsequent use.
Calabash leaf: bitter in the mouth, pungent, warm in nature; Relieving cough and asthma, diuretic dissipating blood stasis; For cough and asthma, edema, postnatal blood stasis is suffered from abdominal pain; Fracture is controlled in external.
Caulis Kadsurae Coccineae: acrid in the mouth, micro-hardship, warm in nature; Promoting the circulation of QI to relieve pain, dispelling, collateral-activating, dissipating blood stasis for subsidence of swelling; For taste-blindness rate, chronic gastritis, acute gastroenteritis, rheumatic arthritis, treating swelling and pain by traumatic injury, dysmenorrhea, postnatal blood stasis is suffered from abdominal pain.
Radix Gynurae bicoloris: lightly seasoned, warm in nature, nontoxic.Promoting flow of QI and blood, stops stasis of blood stomachache, metrorrhagia, malaria after managing property, vim and vigour pain.
Herba Violae Inconspicui: bitter in the mouth, pungent, cold in nature; Heat-clearing and toxic substances removing, removing heat from blood is subsided a swelling, dampness removing blood stasis dispelling; Main furuncle carbuncle, laryngopharynx swelling and pain, acute mastitis, jaundice due to damp-heat, conjunctival congestion corneal nebula, acute appendicitis hematochezia, traumatic injury, traumatic hemorrhage, women's postnatal blood stasis is suffered from abdominal pain, snake bite and insect sting.
Lignum Ormosiae henryi: acrid in the mouth, warm in nature, nontoxic; Expelling wind and removing dampness, promoting blood circulation and breaking stagnation, removing toxic substances and promoting subsidence of swelling; Main air wet arthritis, lumbar muscle strain, postnatal blood stasis is suffered from abdominal pain, multicoloured leukorrhagia, traumatic injury, fracture, flu, venom, innominate toxic swelling.
Herba Thlaspis: bitter in the mouth; Sweet; Cold nature; Heat-clearing and toxic substances removing, inducing diuresis to remove edema; Main conjunctival congestion and swelling pain, lung abscess, acute appendicitis, has loose bowels, dysentery, leucorrhea, and postnatal blood stasis is suffered from abdominal pain, dyspepsia, oedema due to nephritis, cirrhotic ascites, carbuncle pyogenic infections from tumour or sore.
Xinjiang winged euonymus: bitter in the mouth, cold in nature; Promoting blood circulation to restore menstrual flow, blood-activating analgetic, removing toxic substances and promoting subsidence of swelling; Main amenorrhea, postnatal blood stasis is suffered from abdominal pain, arthralgia, and carbuncle sore is red and swollen.
Herba leonuri pseudoma cranthi: taste abnormal pigmentary deposit on the skin, property is put down; Promoting blood flow to regulate menstruation, removing toxic substances and promoting subsidence of swelling; Main menoxenia, amenorrhea, dysmenorrhea, postnatal blood stasis is suffered from abdominal pain, metrorrhagia, traumatic pain, carbuncle sore.
Herba Lycopi: bitter in the mouth, pungent, slightly warm in nature; Blood circulation promoting and blood stasis dispelling, line water detumescence; For menoxenia, amenorrhea, dysmenorrhea, postnatal blood stasis is suffered from abdominal pain, edema.
Palm leaf banyan: sweet in the mouth, micro-hardship, warm in nature; Expelling wind and removing dampness, blood circulation promoting and blood stasis dispelling; For rheumatic ostalgia, amenorrhea, postnatal blood stasis is suffered from abdominal pain, leucorrhea, orchitis, traumatic injury.
Radix Callicarpae Bodinieri: acrid in the mouth, property is put down; Promoting blood circulation to restore menstrual flow, expelling wind and dampness; Control menoxenia, bleeding not during menses, postnatal blood stasis is suffered from abdominal pain, affection of exogenous wind-cold, rheumatalgia.
Alpine yarrow: lightly seasoned, property is put down; Heat-clearing and toxic substances removing, removing blood stasis to reduce swelling, pain relieving; For venom, traumatic injury, postnatal blood stasis is suffered from abdominal pain; External is controlled furuncle and is swollen and ache.
Massa Medicata Fermentata: property is put down, sweet in the mouth; Invigorating the spleen and regulating the stomach, helps digestion in adjusting; Control retention of food and drink, feeling of stuffiness in chest abdominal distention, vomiting dysentery, postnatal blood stasis is suffered from abdominal pain, and children's's enlarged abdomen heavily fortified point is long-pending.
Female ground beetle: salty in the mouth, cold in nature; Removing blood stasis, removing mass, dredging collateral, reason wound; Control lump in the abdomen, amenorrhea due to stagnation of blood, postnatal blood stasis is suffered from abdominal pain, traumatic injury, swollen and rigid tongue, congenital deformity of the tongue.
Charybdis japonica: salty in the mouth, cold in nature, nontoxic; Blood circulation promoting and blood stasis dispelling; Help digestion; Lactogenesis.Main blood stasis amenorrhea; The stagnant stomachache of the stasis of blood in puerperal; Dyspepsia; Food stagnation feeling of fullness; Breast method is not enough.
Herba Leonuri extract: invigorate blood circulation, blood stasis dispelling, regulating menstruation, disappear water; Control menoxenia, vaginal bleeding during pregnancy is had difficult labour, and retention of placenta, puerperal blood faints, abdominal pain due to blood stasis, metrorrhagia and metrostaxis, hematuria, rushes down blood, carbuncle skin infection.
Radix Paeoniae Rubra extract: bitter in the mouth, cold nature; Clearing away heat and cooling blood, blood circulation promoting and blood stasis dispelling; Main maculae caused by violent heat pathogen, hematemesis and epistaxis, discharging fresh blood stool, conjunctival congestion and swelling pain, carbuncle skin infection, amenorrhea, dysmenorrhea, metrorrhagia and leukorrhagia stranguria with turbid discharge, the stagnant hypochondriac pain of the stasis of blood, hernia abdominal mass is gathered, injury from falling down.
Oletum Trogopterori extract: sweet, temperature; Promoting blood circulation to remove blood stasis, parch to black stops blooding; Have a pain for trusted subordinate's congestion, dysmenorrhea, blood stasis amenorrhea, puerperal abdominalgia with blood stasis, parch to black controls metrostaxis, and traumatic injury is controlled in external, Serpentis, insect bite wound.
Detailed description of the invention
Embodiment 1:
Treating a pharmaceutical composition for abdominalgia with blood stasis in puerperal, is be grouped into by the one-tenth of following weight portion: Radix Fici pumilae 89 parts, 76 parts, calabash leaf, Caulis Kadsurae Coccineae 73 parts, Radix Gynurae bicoloris 68 parts, Herba Violae Inconspicui 59 parts, lignum Ormosiae henryi 47 parts, Herba Thlaspis 40 parts, Xinjiang winged euonymus 37 parts, Herba leonuri pseudoma cranthi 31 parts, Herba Lycopi 25 parts, palm leaf banyan 18 parts, Radix Callicarpae Bodinieri 13 parts, alpine yarrow 7 parts, Massa Medicata Fermentata 4 parts, Herba Leonuri extract 5 parts, Radix Paeoniae Rubra extract 2 parts, Oletum Trogopterori extract 1 part, female ground beetle nano powder 6 parts, Charybdis japonica nano powder 4 parts.
Wherein said Herba Leonuri extract is Total alkaloids, its extracting method is: the Herba Leonuri getting fresh harvesting, add the water boiling and extraction 2 times relative to Herba Leonuri 5 times of weight, each extraction 1 hour, merge extractive liquid, then concentrated extracting solution is to water content 35%, then adding relative to the volumetric concentration of concentrated solution 3 times of weight in concentrated solution is the alcoholic solution of 95%, cold preservation 2 hours at 4 DEG C, then ethanol is reclaimed, add wherein volumetric concentration be 3% hydrochloric acid regulate PH to 2, filter, getting filtrate is added on acid cation exchange resin post, first use washed with de-ionized water, be 3% hydrochloric acid cleaning by volumetric concentration again, merge all cleanout fluid, and in cleanout fluid, add the sodium hydroxide solution adjustment PH to 7 that mass concentration is 40%, leave standstill 10min to filter, concentrate filtrate to thick paste, then at 70 DEG C by thick paste drying under reduced pressure, obtain Herba Leonuri extract Total alkaloids.
Wherein said Radix Paeoniae Rubra extract is peoniflorin, and its extracting method is: get Radix Paeoniae Rubra dry product pulverize after cross 100 mesh sieves, then add relative to the volumetric concentration of Radix Paeoniae Rubra powder 3 times of weight be 60% aqueous acetone solution reflux, extract, 3h, filter, filtrate is for subsequent use; Filtering residue uses the aqueous acetone solution reflux, extract, twice of 60% again, extracting solution and above-mentioned filtrate are merged, centrifugal, then by the supernatant silica gel column chromatography of centrifugal gained, gained chromatographic solution is evaporated to the extractum that relative density is 1.05 at 45 DEG C, extractum is dried and is pulverized to constant weight, obtains Radix Paeoniae Rubra extract peoniflorin.
The extracting method that wherein said Oletum Trogopterori extract is is: be placed in by Oletum Trogopterori in dehydrator, dry to moisture lower than 1% for 60 DEG C, taking-up is cooled to room temperature, be ground into coarse powder, be the alcohol heating reflux 2 times of 95% successively by the volumetric concentration relative to Trogopterus Dung 7 times of weight, be respectively 2 hours, 1 hour, merge extractive liquid, is evaporated to extractum at every turn; Add relative to the volumetric concentration of extractum 6 times of weight be 95% dissolve with ethanol extractum, filter, obtain supernatant, then with active carbon particle filter, be the ethanol purge activated carbon granule of 95% by the volumetric concentration relative to supernatant 3 times of weight.Collect ethanol rinse, being concentrated into determining alcohol is 40%, at 24 DEG C, place cold analysis in 8 hours, filters to obtain precipitation, is the washing with alcohol precipitation of 60% by the volumetric concentration relative to precipitation 4 times of weight, drying precipitated, obtains Oletum Trogopterori extract.
The preparation method of wherein said female ground beetle nano powder and Charybdis japonica nano powder is: get Huo female ground beetle and Charybdis japonica respectively, scald dead with boiling water and clean up, then be placed in the inherent 50 DEG C of low temperature of marmite and bake 2 hours, taking-up is cooled to room temperature, first be ground into super micron mill the fine powder that particle diameter is 120 μm, and then obtain 1nm female ground beetle nano powder and Charybdis japonica nano powder respectively after nanoscale pulverization process.
The pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal is made tablet, and its preparation method comprises the following steps:
(1) Radix Fici pumilae is taken by proportioning, calabash leaf, Caulis Kadsurae Coccineae, Radix Gynurae bicoloris, Herba Violae Inconspicui, lignum Ormosiae henryi, Herba Thlaspis, Xinjiang winged euonymus, Herba leonuri pseudoma cranthi, Herba Lycopi, palm leaf banyan, Radix Callicarpae Bodinieri, alpine yarrow, Massa Medicata Fermentata, add the water relative to medical material 10 times of weight, soak 3h, decoct 2 hours, filter, medicinal residues add the water of medical material 8 times of weight again, decoct 1 hour, medicinal liquid leaches, merge twice decoction liquor, being evaporated to 60 DEG C of relative densities is the extractum of 1.05, adding relative to the volumetric concentration of extractum 3 times of weight is the ethanol of 75%, stir, leave standstill, filter, during filtrate reduced in volume to 65 DEG C, relative density is the concentrated solution of 1.05, and reclaim ethanol, concentrated solution is spray dried to extract powder, cross 100 mesh sieves, for subsequent use, spray drying condition is: inlet temperature is 100 DEG C, and leaving air temp is 90 DEG C, and temperature of charge is 70 DEG C, and atomizing pressure is 0.2 MPa, and spray velocity is 10ml/s,
(2) Herba Leonuri of fresh harvesting is got, add the water boiling and extraction 2 times relative to Herba Leonuri 5 times of weight, each extraction 1 hour, merge extractive liquid, then concentrated extracting solution is to water content 35%, then adding relative to the volumetric concentration of concentrated solution 3 times of weight in concentrated solution is the alcoholic solution of 95%, cold preservation 2 hours at 4 DEG C, then ethanol is reclaimed, add wherein volumetric concentration be 3% hydrochloric acid regulate PH to 2, filter, getting filtrate is added on acid cation exchange resin post, first use washed with de-ionized water, be 3% hydrochloric acid cleaning by volumetric concentration again, merge all cleanout fluid, and in cleanout fluid, add the sodium hydroxide solution adjustment PH to 7 that mass concentration is 40%, leave standstill 10min to filter, concentrate filtrate to thick paste, then at 70 DEG C by thick paste drying under reduced pressure, obtain Herba Leonuri extract Total alkaloids, for subsequent use,
(3) get Radix Paeoniae Rubra dry product pulverize after cross 100 mesh sieves, then add relative to the volumetric concentration of Radix Paeoniae Rubra powder 3 times of weight be 60% aqueous acetone solution reflux, extract, 3h, filter, filtrate is for subsequent use; Filtering residue uses the aqueous acetone solution reflux, extract, twice of 60% again, extracting solution and above-mentioned filtrate are merged, centrifugal, then by the supernatant silica gel column chromatography of centrifugal gained, gained chromatographic solution is evaporated to the extractum that relative density is 1.05 at 45 DEG C, extractum is dried and is pulverized to constant weight, obtains Radix Paeoniae Rubra extract peoniflorin, for subsequent use;
(4) Oletum Trogopterori is placed in dehydrator, dry to moisture lower than 1% for 60 DEG C, taking-up is cooled to room temperature, be ground into coarse powder, be the alcohol heating reflux 2 times of 95% successively by the volumetric concentration relative to Trogopterus Dung 7 times of weight, be respectively 2 hours, 1 hour, merge extractive liquid, is evaporated to extractum at every turn; Add relative to the volumetric concentration of extractum 6 times of weight be 95% dissolve with ethanol extractum, filter, obtain supernatant, then with active carbon particle filter, be the ethanol purge activated carbon granule of 95% by the volumetric concentration relative to supernatant 3 times of weight.Collect ethanol rinse, being concentrated into determining alcohol is 40%, at 24 DEG C, place cold analysis in 8 hours, filters to obtain precipitation, is the washing with alcohol precipitation of 60% by the volumetric concentration relative to precipitation 4 times of weight, drying precipitated, obtains Oletum Trogopterori extract, for subsequent use;
(5) Huo female ground beetle and Charybdis japonica is got respectively, scald dead with boiling water and clean up, then be placed in the inherent 50 DEG C of low temperature of marmite and bake 2 hours, taking-up is cooled to room temperature, first be ground into super micron mill the fine powder that particle diameter is 120 μm, and then obtain 1nm female ground beetle nano powder and Charybdis japonica nano powder respectively after nanoscale pulverization process;
(6) Herba Leonuri extract, Radix Paeoniae Rubra extract, Oletum Trogopterori extract, female ground beetle nano powder and Charybdis japonica nano powder is taken by proportioning, mix homogeneously in dispensing canister with the extract powder of step (1) gained, the pharmaceutical composition of abdominalgia with blood stasis in puerperal must be treated;
(7) in the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal, 12% pregelatinized Starch, 3% microcrystalline Cellulose, 0.1% BHA, 0.2% magnesium stearate is added, mixing, granulation, tabletting, specification is, 0.8g/ sheet, obtains the tablet of the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal.
Embodiment 2:
Treating a pharmaceutical composition for abdominalgia with blood stasis in puerperal, is be grouped into by the one-tenth of following weight portion: Radix Fici pumilae 96 parts, 84 parts, calabash leaf, Caulis Kadsurae Coccineae 80 parts, Radix Gynurae bicoloris 76 parts, Herba Violae Inconspicui 67 parts, lignum Ormosiae henryi 56 parts, Herba Thlaspis 47 parts, Xinjiang winged euonymus 41 parts, Herba leonuri pseudoma cranthi 35 parts, Herba Lycopi 29 parts, palm leaf banyan 22 parts, Radix Callicarpae Bodinieri 16 parts, alpine yarrow 11 parts, Massa Medicata Fermentata 5 parts, Herba Leonuri extract 6.5 parts, Radix Paeoniae Rubra extract 3 parts, Oletum Trogopterori extract 2 parts, female ground beetle nano powder 7.5 parts, Charybdis japonica nano powder 5.5 parts.
Wherein said Herba Leonuri extract is Total alkaloids, its extracting method is: the Herba Leonuri getting fresh harvesting, add the water boiling and extraction 2 times relative to Herba Leonuri 5 times of weight, each extraction 1.5 hours, merge extractive liquid, then concentrated extracting solution is to water content 35%, then adding relative to the volumetric concentration of concentrated solution 3 times of weight in concentrated solution is the alcoholic solution of 95%, cold preservation 2 hours at 5 DEG C, then ethanol is reclaimed, add wherein volumetric concentration be 3% hydrochloric acid regulate PH to 2.5, filter, getting filtrate is added on acid cation exchange resin post, first use washed with de-ionized water, be 3% hydrochloric acid cleaning by volumetric concentration again, merge all cleanout fluid, and in cleanout fluid, add the sodium hydroxide solution adjustment PH to 7 that mass concentration is 40%, leave standstill 10min to filter, concentrate filtrate to thick paste, then at 75 DEG C by thick paste drying under reduced pressure, obtain Herba Leonuri extract Total alkaloids.
Wherein said Radix Paeoniae Rubra extract is peoniflorin, and its extracting method is: get Radix Paeoniae Rubra dry product pulverize after cross 100 mesh sieves, then add relative to the volumetric concentration of Radix Paeoniae Rubra powder 3 times of weight be 60% aqueous acetone solution reflux, extract, 3h, filter, filtrate is for subsequent use; Filtering residue uses the aqueous acetone solution reflux, extract, twice of 60% again, extracting solution and above-mentioned filtrate are merged, centrifugal, then by the supernatant silica gel column chromatography of centrifugal gained, gained chromatographic solution is evaporated to the extractum that relative density is 1.15 at 60 DEG C, extractum is dried and is pulverized to constant weight, obtains Radix Paeoniae Rubra extract peoniflorin.
The extracting method of wherein said Oletum Trogopterori extract is: be placed in by Oletum Trogopterori in dehydrator, dry to moisture lower than 1% for 65 DEG C, taking-up is cooled to room temperature, be ground into coarse powder, be the alcohol heating reflux 2 times of 95% successively by the volumetric concentration relative to Trogopterus Dung 7 times of weight, be respectively 2 hours, 1 hour, merge extractive liquid, is evaporated to extractum at every turn; Add relative to the volumetric concentration of extractum 6 times of weight be 95% dissolve with ethanol extractum, filter, obtain supernatant, then with active carbon particle filter, be the ethanol purge activated carbon granule of 95% by the volumetric concentration relative to supernatant 3 times of weight.Collect ethanol rinse, being concentrated into determining alcohol is 40%, at 24 DEG C, place cold analysis in 8 hours, filters to obtain precipitation, is the washing with alcohol precipitation of 60% by the volumetric concentration relative to precipitation 4 times of weight, drying precipitated, obtains Oletum Trogopterori extract.
The preparation method of wherein said female ground beetle nano powder and Charybdis japonica nano powder is: get Huo female ground beetle and Charybdis japonica respectively, scald dead with boiling water and clean up, then be placed in the inherent 60 DEG C of low temperature of marmite and bake 2.5 hours, taking-up is cooled to room temperature, first be ground into super micron mill the fine powder that particle diameter is 135 μm, and then obtain 50nm female ground beetle nano powder and Charybdis japonica nano powder respectively after nanoscale pulverization process.
The pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal is made tablet, and its preparation method comprises the following steps:
(1) Radix Fici pumilae is taken by proportioning, calabash leaf, Caulis Kadsurae Coccineae, Radix Gynurae bicoloris, Herba Violae Inconspicui, lignum Ormosiae henryi, Herba Thlaspis, Xinjiang winged euonymus, Herba leonuri pseudoma cranthi, Herba Lycopi, palm leaf banyan, Radix Callicarpae Bodinieri, alpine yarrow, Massa Medicata Fermentata, add the water relative to medical material 10 times of weight, soak 4h, decoct 2.5 hours, filter, medicinal residues add the water of medical material 8 times of weight again, decoct 1.5 hours, medicinal liquid leaches, merge twice decoction liquor, being evaporated to 60 DEG C of relative densities is the extractum of 1.05, adding relative to the volumetric concentration of extractum 3 times of weight is the ethanol of 75%, stir, leave standstill, filter, during filtrate reduced in volume to 65 DEG C, relative density is the concentrated solution of 1.15, and reclaim ethanol, concentrated solution is spray dried to extract powder, cross 100 mesh sieves, for subsequent use, spray drying condition is: inlet temperature is 100 DEG C, and leaving air temp is 90 DEG C, and temperature of charge is 70 DEG C, and atomizing pressure is 0.2 MPa, and spray velocity is 10ml/s,
(2) Herba Leonuri of fresh harvesting is got, add the water boiling and extraction 2 times relative to Herba Leonuri 5 times of weight, each extraction 1.5 hours, merge extractive liquid, then concentrated extracting solution is to water content 35%, then adding relative to the volumetric concentration of concentrated solution 3 times of weight in concentrated solution is the alcoholic solution of 95%, cold preservation 2 hours at 5 DEG C, then ethanol is reclaimed, add wherein volumetric concentration be 3% hydrochloric acid regulate PH to 2.5, filter, getting filtrate is added on acid cation exchange resin post, first use washed with de-ionized water, be 3% hydrochloric acid cleaning by volumetric concentration again, merge all cleanout fluid, and in cleanout fluid, add the sodium hydroxide solution adjustment PH to 7 that mass concentration is 40%, leave standstill 10min to filter, concentrate filtrate to thick paste, then at 75 DEG C by thick paste drying under reduced pressure, obtain Herba Leonuri extract Total alkaloids, for subsequent use,
(3) get Radix Paeoniae Rubra dry product pulverize after cross 100 mesh sieves, then add relative to the volumetric concentration of Radix Paeoniae Rubra powder 3 times of weight be 60% aqueous acetone solution reflux, extract, 3h, filter, filtrate is for subsequent use; Filtering residue uses the aqueous acetone solution reflux, extract, twice of 60% again, extracting solution and above-mentioned filtrate are merged, centrifugal, then by the supernatant silica gel column chromatography of centrifugal gained, gained chromatographic solution is evaporated to the extractum that relative density is 1.15 at 60 DEG C, extractum is dried and is pulverized to constant weight, obtains Radix Paeoniae Rubra extract peoniflorin, for subsequent use;
(4) Oletum Trogopterori is placed in dehydrator, dry to moisture lower than 1% for 65 DEG C, taking-up is cooled to room temperature, be ground into coarse powder, be the alcohol heating reflux 2 times of 95% successively by the volumetric concentration relative to Trogopterus Dung 7 times of weight, be respectively 2 hours, 1 hour, merge extractive liquid, is evaporated to extractum at every turn; Add relative to the volumetric concentration of extractum 6 times of weight be 95% dissolve with ethanol extractum, filter, obtain supernatant, then with active carbon particle filter, be the ethanol purge activated carbon granule of 95% by the volumetric concentration relative to supernatant 3 times of weight.Collect ethanol rinse, being concentrated into determining alcohol is 40%, at 24 DEG C, place cold analysis in 8 hours, filters to obtain precipitation, is the washing with alcohol precipitation of 60% by the volumetric concentration relative to precipitation 4 times of weight, drying precipitated, obtains Oletum Trogopterori extract, for subsequent use;
(5) Huo female ground beetle and Charybdis japonica is got respectively, scald dead with boiling water and clean up, then be placed in the inherent 60 DEG C of low temperature of marmite and roast 2.5 hours, taking-up is cooled to room temperature, first be ground into super micron mill the fine powder that particle diameter is 135 μm, and then 50nm female ground beetle nano powder and Charybdis japonica nano powder is obtained respectively after nanoscale pulverization process, for subsequent use;
(6) Herba Leonuri extract, Radix Paeoniae Rubra extract, Oletum Trogopterori extract, female ground beetle nano powder and Charybdis japonica nano powder is taken by proportioning, mix homogeneously in dispensing canister with the extract powder of step (1) gained, the pharmaceutical composition of abdominalgia with blood stasis in puerperal must be treated;
(7) in the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal, 16% pregelatinized Starch, 4% microcrystalline Cellulose, 0.15% BHA, 0.25% magnesium stearate is added, mixing, granulation, tabletting, specification is 0.9g/ sheet, obtains the tablet of the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal.
Embodiment 3:
Treating a pharmaceutical composition for abdominalgia with blood stasis in puerperal, is be grouped into by the one-tenth of following weight portion: Radix Fici pumilae 103 parts, 90 parts, calabash leaf, Caulis Kadsurae Coccineae 87 parts, Radix Gynurae bicoloris 84 parts, Herba Violae Inconspicui 77 parts, lignum Ormosiae henryi 65 parts, Herba Thlaspis 54 parts, Xinjiang winged euonymus 45 parts, Herba leonuri pseudoma cranthi 39 parts, Herba Lycopi 33 parts, palm leaf banyan 26 parts, Radix Callicarpae Bodinieri 19 parts, alpine yarrow 15 parts, Massa Medicata Fermentata 6 parts, Herba Leonuri extract 8 parts, Radix Paeoniae Rubra extract 4 parts, Oletum Trogopterori extract 3 parts, female ground beetle nano powder 9 parts, Charybdis japonica nano powder 7 parts.
Wherein said Herba Leonuri extract is Total alkaloids, its extracting method is: the Herba Leonuri getting fresh harvesting, add the water boiling and extraction 3 times relative to Herba Leonuri 5 times of weight, each extraction 2 hours, merge extractive liquid, then concentrated extracting solution is to water content 35%, then adding relative to the volumetric concentration of concentrated solution 3 times of weight in concentrated solution is the alcoholic solution of 95%, cold preservation 2 hours at 6 DEG C, then ethanol is reclaimed, add wherein volumetric concentration be 3% hydrochloric acid regulate PH to 3, filter, getting filtrate is added on acid cation exchange resin post, first use washed with de-ionized water, be 3% hydrochloric acid cleaning by volumetric concentration again, merge all cleanout fluid, and in cleanout fluid, add the sodium hydroxide solution adjustment PH to 7 that mass concentration is 40%, leave standstill 10min to filter, concentrate filtrate to thick paste, then at 80 DEG C by thick paste drying under reduced pressure, obtain Herba Leonuri extract Total alkaloids.
Wherein said Radix Paeoniae Rubra extract is peoniflorin, and its extracting method is: get Radix Paeoniae Rubra dry product pulverize after cross 100 mesh sieves, then add relative to the volumetric concentration of Radix Paeoniae Rubra powder 3 times of weight be 60% aqueous acetone solution reflux, extract, 3h, filter, filtrate is for subsequent use; Filtering residue uses the aqueous acetone solution reflux, extract, twice of 60% again, extracting solution and above-mentioned filtrate are merged, centrifugal, then by the supernatant silica gel column chromatography of centrifugal gained, gained chromatographic solution is evaporated to the extractum that relative density is 1.25 at 75 DEG C, extractum is dried and is pulverized to constant weight, obtains Radix Paeoniae Rubra extract peoniflorin.
The extracting method of wherein said Oletum Trogopterori extract is: be placed in by Oletum Trogopterori in dehydrator, dry to moisture lower than 1% for 70 DEG C, taking-up is cooled to room temperature, be ground into coarse powder, be the alcohol heating reflux 2 times of 95% successively by the volumetric concentration relative to Trogopterus Dung 7 times of weight, be respectively 2 hours, 1 hour, merge extractive liquid, is evaporated to extractum at every turn; Add relative to the volumetric concentration of extractum 6 times of weight be 95% dissolve with ethanol extractum, filter, obtain supernatant, then with active carbon particle filter, be the ethanol purge activated carbon granule of 95% by the volumetric concentration relative to supernatant 3 times of weight.Collect ethanol rinse, being concentrated into determining alcohol is 40%, at 24 DEG C, place cold analysis in 8 hours, filters to obtain precipitation, is the washing with alcohol precipitation of 60% by the volumetric concentration relative to precipitation 4 times of weight, drying precipitated, obtains Oletum Trogopterori extract.
The preparation method of wherein said female ground beetle nano powder and Charybdis japonica nano powder is: get Huo female ground beetle and each 50 of Charybdis japonica respectively, scald dead with boiling water and clean up, then be placed in the inherent 70 DEG C of low temperature of marmite and bake 3 hours, taking-up is cooled to room temperature, first be ground into super micron mill the fine powder that particle diameter is 150 μm, and then obtain 100nm female ground beetle nano powder and Charybdis japonica nano powder respectively after nanoscale pulverization process.
The pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal is made tablet, and its preparation method comprises the following steps:
(1) Radix Fici pumilae is taken by proportioning, calabash leaf, Caulis Kadsurae Coccineae, Radix Gynurae bicoloris, Herba Violae Inconspicui, lignum Ormosiae henryi, Herba Thlaspis, Xinjiang winged euonymus, Herba leonuri pseudoma cranthi, Herba Lycopi, palm leaf banyan, Radix Callicarpae Bodinieri, alpine yarrow, Massa Medicata Fermentata, add the water relative to medical material 10 times of weight, soak 5h, decoct 3 hours, filter, medicinal residues add the water of medical material 8 times of weight again, decoct 2 hours, medicinal liquid leaches, merge twice decoction liquor, being evaporated to 60 DEG C of relative densities is the extractum of 1.05, adding relative to the volumetric concentration of extractum 3 times of weight is the ethanol of 75%, stir, leave standstill, filter, during filtrate reduced in volume to 65 DEG C, relative density is the concentrated solution of 1.25, and reclaim ethanol, concentrated solution is spray dried to extract powder, cross 100 mesh sieves, for subsequent use, spray drying condition is: inlet temperature is 100 DEG C, and leaving air temp is 90 DEG C, and temperature of charge is 70 DEG C, and atomizing pressure is 0.2 MPa, and spray velocity is 10ml/s,
(2) Herba Leonuri of fresh harvesting is got, add the water boiling and extraction 3 times relative to Herba Leonuri 5 times of weight, each extraction 2 hours, merge extractive liquid, then concentrated extracting solution is to water content 35%, then adding relative to the volumetric concentration of concentrated solution 3 times of weight in concentrated solution is the alcoholic solution of 95%, cold preservation 2 hours at 6 DEG C, then ethanol is reclaimed, add wherein volumetric concentration be 3% hydrochloric acid regulate PH to 3, filter, getting filtrate is added on acid cation exchange resin post, first use washed with de-ionized water, be 3% hydrochloric acid cleaning by volumetric concentration again, merge all cleanout fluid, and in cleanout fluid, add the sodium hydroxide solution adjustment PH to 7 that mass concentration is 40%, leave standstill 10min to filter, concentrate filtrate to thick paste, then at 80 DEG C by thick paste drying under reduced pressure, obtain Herba Leonuri extract Total alkaloids, for subsequent use,
(3) get Radix Paeoniae Rubra dry product pulverize after cross 100 mesh sieves, then add relative to the volumetric concentration of Radix Paeoniae Rubra powder 3 times of weight be 60% aqueous acetone solution reflux, extract, 3h, filter, filtrate is for subsequent use; Filtering residue uses the aqueous acetone solution reflux, extract, twice of 60% again, extracting solution and above-mentioned filtrate are merged, centrifugal, then by the supernatant silica gel column chromatography of centrifugal gained, gained chromatographic solution is evaporated to the extractum that relative density is 1.25 at 75 DEG C, extractum is dried and is pulverized to constant weight, obtains Radix Paeoniae Rubra extract peoniflorin, for subsequent use;
(4) Oletum Trogopterori is placed in dehydrator, dry to moisture lower than 1% for 70 DEG C, taking-up is cooled to room temperature, be ground into coarse powder, be the alcohol heating reflux 2 times of 95% successively by the volumetric concentration relative to Trogopterus Dung 7 times of weight, be respectively 2 hours, 1 hour, merge extractive liquid, is evaporated to extractum at every turn; Add relative to the volumetric concentration of extractum 6 times of weight be 95% dissolve with ethanol extractum, filter, obtain supernatant, then with active carbon particle filter, be the ethanol purge activated carbon granule of 95% by the volumetric concentration relative to supernatant 3 times of weight.Collect ethanol rinse, being concentrated into determining alcohol is 40%, at 24 DEG C, place cold analysis in 8 hours, filters to obtain precipitation, is the washing with alcohol precipitation of 60% by the volumetric concentration relative to precipitation 4 times of weight, drying precipitated, obtains Oletum Trogopterori extract, for subsequent use;
(5) Huo female ground beetle and Charybdis japonica is got respectively, scald dead with boiling water and clean up, then be placed in the inherent 70 DEG C of low temperature of marmite and bake 3 hours, taking-up is cooled to room temperature, first be ground into super micron mill the fine powder that particle diameter is 150 μm, and then 100nm female ground beetle nano powder and Charybdis japonica nano powder is obtained respectively after nanoscale pulverization process, for subsequent use;
(6) Herba Leonuri extract, Radix Paeoniae Rubra extract, Oletum Trogopterori extract, female ground beetle nano powder and Charybdis japonica nano powder is taken by proportioning, mix homogeneously in dispensing canister with the extract powder of step (1) gained, the pharmaceutical composition of abdominalgia with blood stasis in puerperal must be treated;
(7) in the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal, 20% pregelatinized Starch, 5% microcrystalline Cellulose, 0.2% BHA, 0.3% magnesium stearate is added, mixing, granulation, tabletting, specification is 1.0g/ sheet, obtains the tablet of the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal.
Clinical treatment case:
1. treatment target: choose abdominalgia with blood stasis patient 84 example in puerperal that certain hospital accepts for medical treatment, be divided into three groups at random, often organize 28 people; Disease is seen: lower abdominal pain, lochia amount are few, and not smooth not smooth, color purple secretly often presss from both sides clot, or double sternal rib pain, and complexion is bluish white, and extremity do not warm up, and body of the tongue is dim, thin fur whiten, stringy and tense pulse etc.
2. Therapeutic Method: above 3 groups of patients take the pharmaceutical composition for the treatment of abdominalgia with blood stasis in puerperal prepared by the embodiment of the present invention 1 to 3 respectively, daily three times, each 2, take 2 weeks continuously.
3. therapeutic outcome:
Treat therapeutic outcome statistics after a week
Group | Recovery from illness | Effectively | Invalid | Cure rate | Effective percentage |
Embodiment 1 | 21 | 5 | 2 | 75% | 92,9% |
Embodiment 2 | 24 | 3 | 1 | 85.7% | 96,4% |
Embodiment 3 | 23 | 3 | 2 | 82.1% | 92,9% |
Wherein criterion of therapeutical effect is:
Recovery from illness: all symptoms all disappear, lower abdomen pain disappears, and lochia amount recovers normal, and complexion recovers normal, and extremity are no longer ice-cold;
Effective: all symptoms all alleviate, lower abdomen occasional pain, lochia amount recovers normal, and complexion recovers ruddy, and extremity are also no longer ice-cold;
Invalid: all symptoms are not all improved.
4. conclusion: in treatment clinical course, the patient accepting Drug therapy of the present invention finds no any untoward reaction, from upper table data, the effective percentage of medicine of the present invention to abdominalgia with blood stasis in puerperal reaches more than 90.0%, be the safe and effective medicine for the treatment of abdominalgia with blood stasis in puerperal, there is good clinical meaning.
Typical case:
1. poplar, female, 29 years old, natural labor, in two weeks puerperal, just started to feel abdominal part occasional pain, finds that lochia amount also continues to reduce after couple of days, and sensation cold limbs, and complexion is turned to be yellow, and stomachache increases the weight of; The pharmaceutical composition one week rear stomachache taking treatment abdominalgia with blood stasis in puerperal prepared by the embodiment of the present invention 1 alleviates, and lochia amount recovers normal, and extremity are no longer ice-cold, and complexion also recovers ruddy, continues to take all symptoms after two weeks and all recovers as usual; And the baby of suckling is not also affected, follow up a case by regular visits to after two weeks and have no recurrence.
2. revive certain, female, 34 years old, natural labor, feel that abdominal part has a dull ache puerperal, lochia amount is also few especially always, face jaundice, cold limbs, the pharmaceutical composition taking treatment abdominalgia with blood stasis in puerperal prepared by the embodiment of the present invention 2 two days later lochia amount recovers normal, complexion starts to take a turn for the better to some extent, extremity temperature edges up, and take all symptoms after a week and all disappear, and the mental status is also better than before, and the baby of suckling is not also affected, follow up a case by regular visits to after three weeks and have no recurrence.
3. Deng, female, 25 years old, cesarean, because patient's own bodies is weak, in addition cesarean, lie in bed after production always, puerperal, abdominal pain was serious, doctor once thought wound infection, and patient's lochia amount is little, complexion is turned to be yellow, lip bark, trick is ice-cold, take the pharmaceutical composition for the treatment of abdominalgia with blood stasis in puerperal prepared by the embodiment of the present invention 3 after one week, the obvious lips moist of patient, have rosy cheeks, and stomachache obviously alleviates, lochia amount is busy increase also, after continuing to take three weeks, the all symptoms of patient all disappear, and wound recovery is fine, out-of-bed activity that can be a small amount of, and the baby of suckling is not also affected, follow up a case by regular visits to after three weeks and have no recurrence.
Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in previous embodiment, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (9)
1. treat the pharmaceutical composition of abdominalgia with blood stasis in puerperal for one kind, it is characterized in that, component meter by weight, mainly consist of the following composition: Radix Fici pumilae 89 ~ 103 parts, 76 ~ 90 parts, calabash leaf, Caulis Kadsurae Coccineae 73 ~ 87 parts, Radix Gynurae bicoloris 68 ~ 84 parts, Herba Violae Inconspicui 59 ~ 77 parts, lignum Ormosiae henryi 47 ~ 65 parts, Herba Thlaspis 40 ~ 54 parts, Xinjiang winged euonymus 37 ~ 45 parts, Herba leonuri pseudoma cranthi 31 ~ 39 parts, Herba Lycopi 25 ~ 33 parts, palm leaf banyan 18 ~ 26 parts, Radix Callicarpae Bodinieri 13 ~ 19 parts, alpine yarrow 7 ~ 15 parts, Massa Medicata Fermentata 4 ~ 6 parts, Herba Leonuri extract 5 ~ 8 parts, Radix Paeoniae Rubra extract 2 ~ 4 parts, Oletum Trogopterori extract 1 ~ 3 part, female ground beetle nano powder 6 ~ 9 parts, Charybdis japonica nano powder 4 ~ 7 parts.
2. a kind of pharmaceutical composition for the treatment of abdominalgia with blood stasis in puerperal as claimed in claim 1, it is characterized in that, component meter by weight, mainly consists of the following composition: Radix Fici pumilae 96 parts, 84 parts, calabash leaf, Caulis Kadsurae Coccineae 80 parts, Radix Gynurae bicoloris 76 parts, Herba Violae Inconspicui 67 parts, lignum Ormosiae henryi 56 parts of, Herba Thlaspiss 47 parts, Xinjiang winged euonymus 41 parts, Herba leonuri pseudoma cranthi 35 parts, Herba Lycopi 29 parts, palm leaf banyan 22 parts, Radix Callicarpae Bodinieri 16 parts, alpine yarrow 11 parts, Massa Medicata Fermentata 5 parts, Herba Leonuri extract 6.5 parts, Radix Paeoniae Rubra extract 3 parts, Oletum Trogopterori extract 2 Fen, female ground beetle nano powder 7.5 parts, Charybdis japonica nano powder 5.5 parts.
3. a kind of pharmaceutical composition for the treatment of abdominalgia with blood stasis in puerperal as claimed in claim 1 or 2, it is characterized in that, described Herba Leonuri extract is Total alkaloids, its extracting method is: the Herba Leonuri getting fresh harvesting, add the water boiling and extraction 2 ~ 3 times relative to Herba Leonuri 5 times of weight, each extraction 1 ~ 2 hour, merge extractive liquid, then concentrated extracting solution is to water content 35%, then adding relative to the volumetric concentration of concentrated solution 3 times of weight in concentrated solution is the alcoholic solution of 95%, cold preservation 2 hours at 4 ~ 6 DEG C, then ethanol is reclaimed, add wherein volumetric concentration be 3% hydrochloric acid regulate PH to 2 ~ 3, filter, getting filtrate is added on acid cation exchange resin post, first use washed with de-ionized water, be 3% hydrochloric acid cleaning by volumetric concentration again, merge all cleanout fluid, and in cleanout fluid, add the sodium hydroxide solution adjustment PH to 7 that mass concentration is 40%, leave standstill 10min to filter, concentrate filtrate to thick paste, then at 70 ~ 80 DEG C by thick paste drying under reduced pressure, obtain Herba Leonuri extract Total alkaloids.
4. a kind of pharmaceutical composition for the treatment of abdominalgia with blood stasis in puerperal as claimed in claim 1 or 2, it is characterized in that, described Radix Paeoniae Rubra extract is peoniflorin, its extracting method is: get after Radix Paeoniae Rubra dry product is pulverized and cross 100 mesh sieves, then add relative to the volumetric concentration of Radix Paeoniae Rubra powder 3 times of weight be 60% aqueous acetone solution reflux, extract, 3h, filter, filtrate is for subsequent use; Filtering residue uses the aqueous acetone solution reflux, extract, twice of 60% again, extracting solution and above-mentioned filtrate are merged, centrifugal, then by the supernatant silica gel column chromatography of centrifugal gained, gained chromatographic solution is evaporated to the extractum that relative density is 1.05 ~ 1.25 at 45 ~ 75 DEG C, extractum is dried and is pulverized to constant weight, obtains Radix Paeoniae Rubra extract peoniflorin.
5. a kind of pharmaceutical composition for the treatment of abdominalgia with blood stasis in puerperal as claimed in claim 1 or 2, it is characterized in that, the extracting method of described Oletum Trogopterori extract is: be placed in by Oletum Trogopterori in dehydrator, dry to moisture lower than 1%, take out and be cooled to room temperature, be ground into coarse powder for 60 ~ 70 DEG C, be the alcohol heating reflux 2 times of 95% successively by the volumetric concentration relative to Trogopterus Dung 7 times of weight, be respectively 2 hours, 1 hour, merge extractive liquid, is evaporated to extractum at every turn; Add relative to the volumetric concentration of extractum 6 times of weight be 95% dissolve with ethanol extractum, filter, obtain supernatant, then with active carbon particle filter, be the ethanol purge activated carbon granule of 95% by the volumetric concentration relative to supernatant 3 times of weight.Collect ethanol rinse, being concentrated into determining alcohol is 40%, at 24 DEG C, place cold analysis in 8 hours, filters to obtain precipitation, is the washing with alcohol precipitation of 60% by the volumetric concentration relative to precipitation 4 times of weight, drying precipitated, obtains Oletum Trogopterori extract.
6. a kind of pharmaceutical composition for the treatment of abdominalgia with blood stasis in puerperal as claimed in claim 1 or 2, it is characterized in that, the preparation method of Suo Shu female ground beetle nano powder and Charybdis japonica nano powder is: get Huo female ground beetle and Charybdis japonica respectively, scald dead with boiling water and clean up, then be placed in 50 ~ 70 DEG C, marmite inherence low temperature and bake 2 ~ 3 hours, taking-up is cooled to room temperature, first be ground into super micron mill the fine powder that particle diameter is 120 ~ 150 μm, and then obtain 1 ~ 100nm female ground beetle nano powder and Charybdis japonica nano powder respectively after nanoscale pulverization process.
7. a kind of preparation method for the treatment of the pharmaceutical composition of abdominalgia with blood stasis in puerperal as described in claim 1-6 any one, is characterized in that, comprise the following steps:
(1) Radix Fici pumilae is taken by proportioning, calabash leaf, Caulis Kadsurae Coccineae, Radix Gynurae bicoloris, Herba Violae Inconspicui, lignum Ormosiae henryi, Herba Thlaspis, Xinjiang winged euonymus, Herba leonuri pseudoma cranthi, Herba Lycopi, palm leaf banyan, Radix Callicarpae Bodinieri, alpine yarrow, Massa Medicata Fermentata, add the water relative to medical material 10 times of weight, soak 3 ~ 5h, decoct 2 ~ 3 hours, filter, medicinal residues add the water of medical material 8 times of weight again, decoct 1 ~ 2 hour, medicinal liquid leaches, merge twice decoction liquor, being evaporated to 60 DEG C of relative densities is the extractum of 1.05, adding relative to the volumetric concentration of extractum 3 times of weight is the ethanol of 75%, stir, leave standstill, filter, during filtrate reduced in volume to 65 DEG C, relative density is the concentrated solution of 1.05 ~ 1.25, and reclaim ethanol, concentrated solution is spray dried to extract powder, cross 100 mesh sieves, for subsequent use,
(2) Herba Leonuri of fresh harvesting is got, add the water boiling and extraction 2 ~ 3 times relative to Herba Leonuri 5 times of weight, each extraction 1 ~ 2 hour, merge extractive liquid, then concentrated extracting solution is to water content 35%, then adding relative to the volumetric concentration of concentrated solution 3 times of weight in concentrated solution is the alcoholic solution of 95%, cold preservation 2 hours at 4 ~ 6 DEG C, then ethanol is reclaimed, add wherein volumetric concentration be 3% hydrochloric acid regulate PH to 2 ~ 3, filter, getting filtrate is added on acid cation exchange resin post, first use washed with de-ionized water, be 3% hydrochloric acid cleaning by volumetric concentration again, merge all cleanout fluid, and in cleanout fluid, add the sodium hydroxide solution adjustment PH to 7 that mass concentration is 40%, leave standstill 10min to filter, concentrate filtrate to thick paste, then at 70 ~ 80 DEG C by thick paste drying under reduced pressure, obtain Herba Leonuri extract Total alkaloids, for subsequent use,
(3) get Radix Paeoniae Rubra dry product pulverize after cross 100 mesh sieves, then add relative to the volumetric concentration of Radix Paeoniae Rubra powder 3 times of weight be 60% aqueous acetone solution reflux, extract, 3h, filter, filtrate is for subsequent use; Filtering residue uses the aqueous acetone solution reflux, extract, twice of 60% again, extracting solution and above-mentioned filtrate are merged, centrifugal, then by the supernatant silica gel column chromatography of centrifugal gained, gained chromatographic solution is evaporated to the extractum that relative density is 1.05 ~ 1.25 at 45 ~ 75 DEG C, extractum is dried and is pulverized to constant weight, obtains Radix Paeoniae Rubra extract peoniflorin, for subsequent use;
(4) Oletum Trogopterori is placed in dehydrator, dry to moisture lower than 1% for 60 ~ 70 DEG C, taking-up is cooled to room temperature, be ground into coarse powder, be the alcohol heating reflux 2 times of 95% successively by the volumetric concentration relative to Trogopterus Dung 7 times of weight, be respectively 2 hours, 1 hour, merge extractive liquid, is evaporated to extractum at every turn; Add relative to the volumetric concentration of extractum 6 times of weight be 95% dissolve with ethanol extractum, filter, obtain supernatant, then with active carbon particle filter, be the ethanol purge activated carbon granule of 95% by the volumetric concentration relative to supernatant 3 times of weight.Collect ethanol rinse, being concentrated into determining alcohol is 40%, at 24 DEG C, place cold analysis in 8 hours, filters to obtain precipitation, is the washing with alcohol precipitation of 60% by the volumetric concentration relative to precipitation 4 times of weight, drying precipitated, obtains Oletum Trogopterori extract, for subsequent use;
(5) Huo female ground beetle and Charybdis japonica is got respectively, scald dead with boiling water and clean up, then be placed in 50 ~ 70 DEG C, marmite inherence low temperature and bake 2 ~ 3 hours, taking-up is cooled to room temperature, first be ground into super micron mill the fine powder that particle diameter is 120 ~ 150 μm, and then 1 ~ 100nm female ground beetle nano powder and Charybdis japonica nano powder is obtained respectively after nanoscale pulverization process, for subsequent use;
(6) Herba Leonuri extract, Radix Paeoniae Rubra extract, Oletum Trogopterori extract, female ground beetle nano powder and Charybdis japonica nano powder is taken by proportioning, mix homogeneously in dispensing canister with the extract powder of step (1) gained, the pharmaceutical composition of abdominalgia with blood stasis in puerperal must be treated.
8. a kind of preparation method for the treatment of the pharmaceutical composition of abdominalgia with blood stasis in puerperal as claimed in claim 7, is characterized in that, the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal is made tablet.
9. preparation a kind of preparation method for the treatment of the medicinal composition tablets of abdominalgia with blood stasis in puerperal as claimed in claim 8, is characterized in that, comprise the following steps:
(1) Radix Fici pumilae is taken by proportioning, calabash leaf, Caulis Kadsurae Coccineae, Radix Gynurae bicoloris, Herba Violae Inconspicui, lignum Ormosiae henryi, Herba Thlaspis, Xinjiang winged euonymus, Herba leonuri pseudoma cranthi, Herba Lycopi, palm leaf banyan, Radix Callicarpae Bodinieri, alpine yarrow, Massa Medicata Fermentata, add the water relative to medical material 10 times of weight, soak 3 ~ 5h, decoct 2 ~ 3 hours, filter, medicinal residues add the water of medical material 8 times of weight again, decoct 1 ~ 2 hour, medicinal liquid leaches, merge twice decoction liquor, being evaporated to 60 DEG C of relative densities is the extractum of 1.05, adding relative to the volumetric concentration of extractum 3 times of weight is the ethanol of 75%, stir, leave standstill, filter, during filtrate reduced in volume to 65 DEG C, relative density is the concentrated solution of 1.05 ~ 1.25, and reclaim ethanol, concentrated solution is spray dried to extract powder, cross 100 mesh sieves, for subsequent use,
(2) Herba Leonuri of fresh harvesting is got, add the water boiling and extraction 2 ~ 3 times relative to Herba Leonuri 5 times of weight, each extraction 1 ~ 2 hour, merge extractive liquid, then concentrated extracting solution is to water content 35%, then adding relative to the volumetric concentration of concentrated solution 3 times of weight in concentrated solution is the alcoholic solution of 95%, cold preservation 2 hours at 4 ~ 6 DEG C, then ethanol is reclaimed, add wherein volumetric concentration be 3% hydrochloric acid regulate PH to 2 ~ 3, filter, getting filtrate is added on acid cation exchange resin post, first use washed with de-ionized water, be 3% hydrochloric acid cleaning by volumetric concentration again, merge all cleanout fluid, and in cleanout fluid, add the sodium hydroxide solution adjustment PH to 7 that mass concentration is 40%, leave standstill 10min to filter, concentrate filtrate to thick paste, then at 70 ~ 80 DEG C by thick paste drying under reduced pressure, obtain Herba Leonuri extract Total alkaloids, for subsequent use,
(3) get Radix Paeoniae Rubra dry product pulverize after cross 100 mesh sieves, then add relative to the volumetric concentration of Radix Paeoniae Rubra powder 3 times of weight be 60% aqueous acetone solution reflux, extract, 3h, filter, filtrate is for subsequent use; Filtering residue uses the aqueous acetone solution reflux, extract, twice of 60% again, extracting solution and above-mentioned filtrate are merged, centrifugal, then by the supernatant silica gel column chromatography of centrifugal gained, gained chromatographic solution is evaporated to the extractum that relative density is 1.05 ~ 1.25 at 45 ~ 75 DEG C, extractum is dried and is pulverized to constant weight, obtains Radix Paeoniae Rubra extract peoniflorin, for subsequent use;
(4) Oletum Trogopterori is placed in dehydrator, dry to moisture lower than 1% for 60 ~ 70 DEG C, taking-up is cooled to room temperature, be ground into coarse powder, be the alcohol heating reflux 2 times of 95% successively by the volumetric concentration relative to Trogopterus Dung 7 times of weight, be respectively 2 hours, 1 hour, merge extractive liquid, is evaporated to extractum at every turn; Add relative to the volumetric concentration of extractum 6 times of weight be 95% dissolve with ethanol extractum, filter, obtain supernatant, then with active carbon particle filter, be the ethanol purge activated carbon granule of 95% by the volumetric concentration relative to supernatant 3 times of weight.Collect ethanol rinse, being concentrated into determining alcohol is 40%, at 24 DEG C, place cold analysis in 8 hours, filters to obtain precipitation, is the washing with alcohol precipitation of 60% by the volumetric concentration relative to precipitation 4 times of weight, drying precipitated, obtains Oletum Trogopterori extract, for subsequent use;
(5) Huo female ground beetle and Charybdis japonica is got respectively, scald dead with boiling water and clean up, then be placed in 50 ~ 70 DEG C, marmite inherence low temperature and bake 2 ~ 3 hours, taking-up is cooled to room temperature, first be ground into super micron mill the fine powder that particle diameter is 120 ~ 150 μm, and then 1 ~ 100nm female ground beetle nano powder and Charybdis japonica nano powder is obtained respectively after nanoscale pulverization process, for subsequent use;
(6) Herba Leonuri extract, Radix Paeoniae Rubra extract, Oletum Trogopterori extract, female ground beetle nano powder and Charybdis japonica nano powder is taken by proportioning, mix homogeneously in dispensing canister with the extract powder of step (1) gained, the pharmaceutical composition of abdominalgia with blood stasis in puerperal must be treated;
(7) in the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal, 12 ~ 20% pregelatinized Starch, 3 ~ 5% microcrystalline Cellulose, 0.1 ~ 0.2% BHA, 0.2 ~ 0.3% magnesium stearate is added, mixing, granulation, tabletting, obtain the tablet of the pharmaceutical composition of described treatment abdominalgia with blood stasis in puerperal.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201511019566.XA CN105560629A (en) | 2015-12-29 | 2015-12-29 | Medicinal composition for treating postpartum abdominalgia with blood stasis, and preparation method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201511019566.XA CN105560629A (en) | 2015-12-29 | 2015-12-29 | Medicinal composition for treating postpartum abdominalgia with blood stasis, and preparation method thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN105560629A true CN105560629A (en) | 2016-05-11 |
Family
ID=55871641
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201511019566.XA Pending CN105560629A (en) | 2015-12-29 | 2015-12-29 | Medicinal composition for treating postpartum abdominalgia with blood stasis, and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105560629A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113663049A (en) * | 2021-09-22 | 2021-11-19 | 中国热带农业科学院热带作物品种资源研究所 | Li nationality medicine postpartum medicated bath effervescent granules and preparation method thereof |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1824113A (en) * | 2005-12-15 | 2006-08-30 | 北京阜康仁生物制药科技有限公司 | Postpartum laonurus medicinal preparation and its new preparation method |
CN103845468A (en) * | 2014-03-14 | 2014-06-11 | 林萍 | Traditional Chinese medicine composition for treating poor postpartum uterus involution |
-
2015
- 2015-12-29 CN CN201511019566.XA patent/CN105560629A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1824113A (en) * | 2005-12-15 | 2006-08-30 | 北京阜康仁生物制药科技有限公司 | Postpartum laonurus medicinal preparation and its new preparation method |
CN103845468A (en) * | 2014-03-14 | 2014-06-11 | 林萍 | Traditional Chinese medicine composition for treating poor postpartum uterus involution |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113663049A (en) * | 2021-09-22 | 2021-11-19 | 中国热带农业科学院热带作物品种资源研究所 | Li nationality medicine postpartum medicated bath effervescent granules and preparation method thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104225138A (en) | Traditional Chinese medicine for treating bedsore through oral administration and external washing and preparation method of traditional Chinese medicine | |
CN104324338A (en) | Nursing patch for bedsore | |
CN103263647B (en) | Traditional Chinese medicine for treating postnatal lochiorrhea and preparation method thereof | |
CN104435326A (en) | Traditional Chinese medicine preparation for treating acute mastitis | |
CN102988946A (en) | Traditional Chinese medicine composition for treating pantalgia after childbirth | |
CN103041328A (en) | Traditional Chinese medicine composition for treating blood stasis type lochiorrhea | |
CN105031013A (en) | Medicine for treating lumbar vertebrae bone hyperplasia and preparation method thereof | |
CN102861160B (en) | Traditional Chinese medicine hemorrhoid elimination decotion | |
CN105250878A (en) | Traditional Chinese medicine for treating sub-acute infective endocarditis | |
CN104707046A (en) | Traditional Chinese medicine for treating pelvic inflammation | |
CN104069443A (en) | Traditional Chinese medicine for treating acute purulent osteomyelitis and preparation method of traditional Chinese medicine | |
CN105560629A (en) | Medicinal composition for treating postpartum abdominalgia with blood stasis, and preparation method thereof | |
CN103550708B (en) | Chinese herbal compound for treating dysmenorrheal | |
CN105749172A (en) | Medicament for treating gastric calculus and preparation thereof | |
CN105435131A (en) | Traditional Chinese medicine composition for treating chronic heart failure and preparation method thereof | |
CN104491670A (en) | Traditional Chinese medicine composition for treating chronic pelvic inflammatory disease | |
CN104352995A (en) | Medicine for treating gall-stone and preparation method thereof | |
CN103623331B (en) | Medicine for treating habitual abortion and preparation method of medicine | |
CN102671132A (en) | Traditional Chinese medicine combination for treating atonic constipation | |
CN106377711A (en) | Traditional Chinese medicine paste capable of treating anorectal diseases and preparation method of traditional Chinese medicine paste | |
CN106310049A (en) | Traditional Chinese medicine granule for treating anorectal diseases and preparation method thereof | |
CN104906449A (en) | Traditional Chinese medicine for treating cervicitis | |
CN105943924A (en) | Traditional Chinese medicine for treating gallbladder fire exuberance syndrome type multiple gall-stone | |
CN106266986A (en) | A kind of Chinese medicine composition for treating hysteromyoma | |
CN105477600A (en) | Drug for treating prolonged lochiorrhea and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160511 |
|
RJ01 | Rejection of invention patent application after publication |