CN105557519A - Test-tube plantlet proliferation culture medium of pear tree somatic embryos - Google Patents
Test-tube plantlet proliferation culture medium of pear tree somatic embryos Download PDFInfo
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- CN105557519A CN105557519A CN201510938584.1A CN201510938584A CN105557519A CN 105557519 A CN105557519 A CN 105557519A CN 201510938584 A CN201510938584 A CN 201510938584A CN 105557519 A CN105557519 A CN 105557519A
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- test
- pear tree
- tube plantlet
- culture medium
- somatic embryo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a test-tube plantlet proliferation culture medium of pear tree somatic embryos. The culture medium comprises 1/2 improved SH, 0.5-1.0mgL<-1> of 6-BA, 1-2mgL<-1> of 2,4-D, 0.3-0.5mgL<-1> of NAA, 6.0-7.0gL<-1> of agar, 30gL<-1> of sucrose and 0.1-0.3% of phytagel and has pH value of 5.8-6.0. The culture medium has the beneficial effects that pear trees difficult to propagate are rapidly propagated through somatic embryogenesis by improving a basic culture medium and components and adjusting the culture conditions; the plant transplanting survival rate is more than or equal to 96%; seedlings grow robustly, thus the problem of germplasm degeneration of good seedlings can be effectively solved, plenty of high-quality purified and rejuvenated seedlings can be also provided for production and an ideal receptor system can be also provided for breeding new varieties through genetic transformation or mutation breeding of the pear trees.
Description
Technical field
The present invention relates to a kind of test-tube plantlet increment medium of pear tree excised leaf, belong to botany field.
Background technology
Pear tree is rose family pear, perennial deciduous fruit tree, and arbor all has extensive distribution in the whole world, and about there are 35 initial specieses in the whole world, is wildly distributed in Europe, Asia and African, classification is divided into east pears and the large class of European pear two.Chinese pear area under cultivation and output are only second to apple.
Pear tree is a kind of seeds with higher Development volue, and at present, the modes of reproduction usually adopted is cottage propagation, and the fungal diseases such as this kind of modes of reproduction easy infection leaf spot, spring and summer, it was subject to again aphid damage, and its virus is serious, blade piebald easily comes off, and improved variety degree reduces; And the good characteristic that the use of seedling inferior causes pear tree intrinsic is lost day by day, this constrains the development of these seeds to a certain extent and applies, therefore, selection, breed good pear tree strain become a very outstanding production problem, tissue-culturing rapid propagation is a kind of effective way of pear tree breeding industrialization, the report of related fields is also more, but about the direct somatic embryos of Pears and regenerate fast numerous system technical research there are no successful report.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of can be effectively the test-tube plantlet increment medium of the pear tree somatic embryo of the pear tree nursery stock that the amount of providing in production is of fine quality greatly, purificate and rejuvenate.
In order to realize foregoing invention object, the present invention is achieved through the following technical solutions:
A test-tube plantlet increment medium for pear tree somatic embryo, is characterized in that, comprise 1/2 improvement SH+0.5 ~ 1.0mgL
-16-BA+1 ~ 2mgL
-12,4-D+0.3 ~ 0.5mgL
-1nAA+6.0 ~ 7.0gL
-1agar+30gL
-1sucrose+0.1 ~ 0.3%phytagel, and pH value is 5.8 ~ 6.0.
The test-tube plantlet increment medium of above-mentioned pear tree somatic embryo, preferably includes 1/2 improvement SH+0.8mgL
-16-BA+1.5mgL
-12,4-D+0.4mgL
-1nAA+6.5gL
-1agar+30gL
-1sucrose+0.2%phytagel, and pH value is 5.9.
Further, described improvement SH comprises grand nutrition element, micronutrient element and organic reagent.
Further, the component of described grand nutrition element is as follows with the concentration of its correspondence:
Potassium nitrate 2100mg/L;
Ammonium sulfate 583mg/L;
Epsom salt 210mg/L;
Calcium chloride dihydrate 220mg/L;
Anhydrous potassium dihydrogenphosphate 380mg/L;
Disodium ethylene diamine tetraacetate 3.5mg/L;
Ferrous sulfate heptahydrate 5.9mg/L.
The component of described micronutrient element is as follows with the concentration of its correspondence:
Four water manganese sulphate 15.2mg/L;
Zinc sulphate 1.5mg/L;
Boric acid 6.2mg/L;
Potassium iodide 1.0mg/L;
Sodium molybdate 0.2mg/L;
Copper sulphate 0.1mg/L;
Cobalt chloride 0.05mg/L.
And the component of described organic reagent and the concentration of its correspondence as follows:
Thiamine hydrochloride 10.0mg/L;
Nicotinic acid 1.0mg/L;
Puridoxine hydrochloride 1.0mg/L;
Inositol 100.0mg/L.
The invention has the beneficial effects as follows: the present invention is by improvement minimal medium and the supplementary measures such as composition, adjustment condition of culture, plantlet of transplant survival rate is made to reach more than 96%, seedling early growth is healthy and strong, effectively can solve good seed kind matter degenerate problem, also can be the pear tree nursery stock that production provides a large amount of high-quality to purificate and rejuvenate, in addition, also can be pear tree genetic transformation or mutation breeding seed selection new varieties and a desirable acceptor systems is provided.
Embodiment
The specific embodiment of the present invention is described in detail below in conjunction with specific embodiment:
embodiment 1
The test-tube plantlet increment medium of pear tree somatic embryo, comprises 1/2 improvement SH+0.5mgL
-16-BA+1mgL
-12,4-D+0.3mgL
-1nAA+6.0gL
-1agar+30gL
-1sucrose+0.1%phytagel, and pH value is 5.8.
embodiment 2
The test-tube plantlet increment medium of pear tree somatic embryo, comprises 1/2 improvement SH+1.0mgL
-16-BA+2mgL
-12,4-D+0.5mgL
-1nAA+7.0gL
-1agar+30gL
-1sucrose+0.3%phytagel, and pH value is 6.0.
embodiment 3
The test-tube plantlet increment medium of pear tree somatic embryo, comprises 1/2 improvement SH+0.8mgL
-16-BA+1.5mgL
-12,4-D+0.4mgL
-1nAA+6.5gL
-1agar+30gL
-1sucrose+0.2%phytagel, and pH value is 5.9.
Above-described embodiment with embodiment 3 for most preferred embodiment.
And in 3 wherein above-mentioned embodiments, improvement SH comprises grand nutrition element, micronutrient element and organic reagent, wherein, the component of grand nutrition element is as follows with the concentration of its correspondence:
Potassium nitrate 2100mg/L;
Ammonium sulfate 583mg/L;
Epsom salt 210mg/L;
Calcium chloride dihydrate 220mg/L;
Anhydrous potassium dihydrogenphosphate 380mg/L;
Disodium ethylene diamine tetraacetate 3.5mg/L;
Ferrous sulfate heptahydrate 5.9mg/L.
The component of described micronutrient element is as follows with the concentration of its correspondence:
Four water manganese sulphate 15.2mg/L;
Zinc sulphate 1.5mg/L;
Boric acid 6.2mg/L;
Potassium iodide 1.0mg/L;
Sodium molybdate 0.2mg/L;
Copper sulphate 0.1mg/L;
Cobalt chloride 0.05mg/L.
And the component of described organic reagent and the concentration of its correspondence as follows:
Thiamine hydrochloride 10.0mg/L;
Nicotinic acid 1.0mg/L;
Puridoxine hydrochloride 1.0mg/L;
Inositol 100.0mg/L.
The present invention utilizes the pear tree of the fast numerous seminal propagation difficulty of hot research plant somatocyte embryo generation technique in plant cell and tissue cultures to be in recent years only the fundamental way solved the problem, it not only can solve the problem that pear tree kind matter is degenerated, reach the object of good seed detoxification, purification and rejuvenation and Fast-propagation, and be the important channel of highly efficient regeneration seed source, still carry out the desirable acceptor material of genetic transformation or mutation breeding seed selection new varieties.
Below disclose the present invention with preferred embodiment, so it is not intended to limiting the invention, and all employings are equal to replacement or the technical scheme that obtains of equivalent transformation mode, all drop within protection scope of the present invention.
Claims (6)
1. a test-tube plantlet increment medium for pear tree somatic embryo, is characterized in that, comprise 1/2 improvement SH+0.5 ~ 1.0mgL
-16-BA+1 ~ 2mgL
-12,4-D+0.3 ~ 0.5mgL
-1nAA+6.0 ~ 7.0gL
-1agar+30gL
-1sucrose+0.1 ~ 0.3%phytagel, and pH value is 5.8 ~ 6.0.
2. a test-tube plantlet increment medium for pear tree somatic embryo, is characterized in that, comprise 1/2 improvement SH+0.8mgL
-16-BA+1.5mgL
-12,4-D+0.4mgL
-1nAA+6.5gL
-1agar+30gL
-1sucrose+0.2%phytagel, and pH value is 5.9.
3. the test-tube plantlet increment medium of a kind of pear tree somatic embryo according to claim 1 and 2, it is characterized in that, described improvement SH comprises grand nutrition element, micronutrient element and organic reagent.
4. the test-tube plantlet increment medium of a kind of pear tree somatic embryo according to claim 3, it is characterized in that, the component of described grand nutrition element is as follows with the concentration of its correspondence:
Potassium nitrate 2100mg/L;
Ammonium sulfate 583mg/L;
Epsom salt 210mg/L;
Calcium chloride dihydrate 220mg/L;
Anhydrous potassium dihydrogenphosphate 380mg/L;
Disodium ethylene diamine tetraacetate 3.5mg/L;
Ferrous sulfate heptahydrate 5.9mg/L.
5. the test-tube plantlet increment medium of a kind of pear tree somatic embryo according to claim 3, it is characterized in that, the component of described micronutrient element is as follows with the concentration of its correspondence:
Four water manganese sulphate 15.2mg/L;
Zinc sulphate 1.5mg/L;
Boric acid 6.2mg/L;
Potassium iodide 1.0mg/L;
Sodium molybdate 0.2mg/L;
Copper sulphate 0.1mg/L;
Cobalt chloride 0.05mg/L.
6. the test-tube plantlet increment medium of a kind of pear tree somatic embryo according to claim 3, it is characterized in that, the component of described organic reagent is as follows with the concentration of its correspondence:
Thiamine hydrochloride 10.0mg/L;
Nicotinic acid 1.0mg/L;
Puridoxine hydrochloride 1.0mg/L;
Inositol 100.0mg/L.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510938584.1A CN105557519A (en) | 2015-12-16 | 2015-12-16 | Test-tube plantlet proliferation culture medium of pear tree somatic embryos |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510938584.1A CN105557519A (en) | 2015-12-16 | 2015-12-16 | Test-tube plantlet proliferation culture medium of pear tree somatic embryos |
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| CN105557519A true CN105557519A (en) | 2016-05-11 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102648698A (en) * | 2012-05-23 | 2012-08-29 | 南京农业大学 | Pyrus stem tip tissue culture rapid propagation method |
| CN102986535A (en) * | 2012-12-14 | 2013-03-27 | 西南林业大学 | Fast propagation method of seedless roxburgh rose seedlings |
| CN104126511A (en) * | 2014-08-22 | 2014-11-05 | 江苏省农业科学院 | Tissue culture method for early-maturing pear stem section and culture medium |
-
2015
- 2015-12-16 CN CN201510938584.1A patent/CN105557519A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102648698A (en) * | 2012-05-23 | 2012-08-29 | 南京农业大学 | Pyrus stem tip tissue culture rapid propagation method |
| CN102986535A (en) * | 2012-12-14 | 2013-03-27 | 西南林业大学 | Fast propagation method of seedless roxburgh rose seedlings |
| CN104126511A (en) * | 2014-08-22 | 2014-11-05 | 江苏省农业科学院 | Tissue culture method for early-maturing pear stem section and culture medium |
Non-Patent Citations (4)
| Title |
|---|
| 傅润民: "《果树瓜类生物工程育种》", 31 May 1994 * |
| 李云等: "《林果花菜组织培养快速育苗技术》", 30 June 2001 * |
| 王定康等: "依主梨树茎尖离体培养", 《云南农业大学学报》 * |
| 金青等: "‘砀山酥梨’的组织培养和脱毒快速繁殖", 《植物生理学通讯》 * |
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Application publication date: 20160511 |
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