CN105379625A - Pear inducer embryo formation medium - Google Patents
Pear inducer embryo formation medium Download PDFInfo
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- CN105379625A CN105379625A CN201510938585.6A CN201510938585A CN105379625A CN 105379625 A CN105379625 A CN 105379625A CN 201510938585 A CN201510938585 A CN 201510938585A CN 105379625 A CN105379625 A CN 105379625A
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- CN
- China
- Prior art keywords
- pear
- inductor
- pear tree
- inducer
- improvement
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention discloses a pear inducer embryo formation medium. The pear inducer embryo formation medium comprises 1/2 of improved MS, 0.5-1.0mgL<-1> of 6-BA, 0.3-0.5mgL<-1> of 2, 4-D, 0.5-1.0mgL<-1> of NAA, 6.0-7.0gL<-1> of agar, 30gL<-1> of cane sugar and 0.1-0.3% of phytagel and has pH of 5.8-6.0. Through improvement of a basic medium and components and adjustment of culture conditions, a pear with breeding difficulty produces approach fast breeding by somatic embryo, a plant transplanting survival rate is 96% or more and a seedling grows strongly. The pear inducer embryo formation medium effectively solves the problem of excellent seedling and germplasm degeneration, provides a lot of high-quality purified renovated nursery stocks for production and provides an ideal acceptor system for pear genetic transformation or mutation breeding for new specie breeding.
Description
Technical field
The present invention relates to a kind of inductor embryogenesis medium of pear tree excised leaf, belong to botany field.
Background technology
Pear tree is rose family pear, perennial deciduous fruit tree, and arbor all has extensive distribution in the whole world, and about there are 35 initial specieses in the whole world, is wildly distributed in Europe, Asia and African, classification is divided into east pears and the large class of European pear two.Chinese pear area under cultivation and output are only second to apple.
Pear tree is a kind of seeds with higher Development volue, and at present, the modes of reproduction usually adopted is cottage propagation, and the fungal diseases such as this kind of modes of reproduction easy infection leaf spot, spring and summer, it was subject to again aphid damage, and its virus is serious, blade piebald easily comes off, and improved variety degree reduces; And the good characteristic that the use of seedling inferior causes pear tree intrinsic is lost day by day, this constrains the development of these seeds to a certain extent and applies, therefore, selection, breed good pear tree strain become a very outstanding production problem, tissue-culturing rapid propagation is a kind of effective way of pear tree breeding industrialization, the report of related fields is also more, but about the direct somatic embryos of Pears and regenerate fast numerous system technical research there are no successful report.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of can be effectively the pear tree inductor embryogenesis medium of the pear tree nursery stock that the amount of providing in production is of fine quality greatly, purificate and rejuvenate.
In order to realize foregoing invention object, the present invention is achieved through the following technical solutions:
A kind of pear tree inductor embryogenesis medium, is characterized in that, comprise 1/2 improvement MS+0.5 ~ 1.0mgL
-16-BA+0.3 ~ 0.5mgL
-12,4-D+0.5 ~ 1.0mgL
-1nAA+6.0 ~ 7.0gL
-1agar+30gL
-1sucrose+0.1 ~ 0.3%phytagel, and pH value is 5.8 ~ 6.0.
A kind of pear tree body inductor embryogenesis medium, preferably includes 1/2 improvement MS+0.8mgL
-16-BA+0.4mgL
-12,4-D+0.8mgL
-1nAA+6.5gL
-1agar+30gL
-1sucrose+0.2%phytagel, and pH value is 6.0.
Further, described improvement MS comprises grand nutrition element, micronutrient element and organic reagent.
Further, the component of described grand nutrition element is as follows with the concentration of its correspondence:
Potassium nitrate 1900mg/L;
Ammonium sulfate 1500mg/L;
Epsom salt 350mg/L;
Calcium chloride dihydrate 420mg/L;
Anhydrous potassium dihydrogenphosphate 185mg/L;
Disodium ethylene diamine tetraacetate 26.8mg/L;
Ferrous sulfate heptahydrate 15.9mg/L.
The component of described micronutrient element is as follows with the concentration of its correspondence:
Four water manganese sulphate 22.5mg/L;
Zinc sulphate 6.8mg/L;
Boric acid 5.4mg/L;
Potassium iodide 1.0mg/L;
Sodium molybdate 0.2mg/L;
Copper sulphate 0.02mg/L;
Cobalt chloride 0.05mg/L.
And the component of described organic reagent and the concentration of its correspondence as follows:
Thiamine hydrochloride 10.0mg/L;
Nicotinic acid 1.0mg/L;
Puridoxine hydrochloride 1.0mg/L;
Inositol 100.0mg/L.
The invention has the beneficial effects as follows: the present invention is by improvement minimal medium and the supplementary measures such as composition, adjustment condition of culture, plantlet of transplant survival rate is made to reach more than 96%, seedling early growth is healthy and strong, effectively can solve good seed kind matter degenerate problem, also can be the pear tree nursery stock that production provides a large amount of high-quality to purificate and rejuvenate, in addition, also can be pear tree genetic transformation or mutation breeding seed selection new varieties and a desirable acceptor systems is provided.
Embodiment
The specific embodiment of the present invention is described in detail below in conjunction with specific embodiment:
embodiment 1
Pear tree inductor embryogenesis medium, comprises 1/2 improvement MS+0.5mgL
-16-BA+0.3mgL
-12,4-D+0.5mgL
-1nAA+6.0gL
-1agar+30gL
-1sucrose+0.1%phytagel, and pH value is 5.8.
embodiment 2
Pear tree inductor embryogenesis medium, comprises 1/2 improvement MS+1.0mgL
-16-BA+0.5mgL
-12,4-D+1.0mgL
-1nAA+7.0gL
-1agar+30gL
-1sucrose+0.3%phytagel, and pH value is 5.9.
embodiment 3
Pear tree body inductor embryogenesis medium, comprises 1/2 improvement MS+0.8mgL
-16-BA+0.4mgL
-12,4-D+0.8mgL
-1nAA+6.5gL
-1agar+30gL
-1sucrose+0.2%phytagel, and pH value is 6.0.
Above-described embodiment with embodiment 3 for most preferred embodiment.
And in 3 wherein above-mentioned embodiments, improvement MS comprises grand nutrition element, micronutrient element and organic reagent, wherein, the component of grand nutrition element is as follows with the concentration of its correspondence:
Potassium nitrate 1900mg/L;
Ammonium sulfate 1500mg/L;
Epsom salt 350mg/L;
Calcium chloride dihydrate 420mg/L;
Anhydrous potassium dihydrogenphosphate 185mg/L;
Disodium ethylene diamine tetraacetate 26.8mg/L;
Ferrous sulfate heptahydrate 15.9mg/L.
The component of described micronutrient element is as follows with the concentration of its correspondence:
Four water manganese sulphate 22.5mg/L;
Zinc sulphate 6.8mg/L;
Boric acid 5.4mg/L;
Potassium iodide 1.0mg/L;
Sodium molybdate 0.2mg/L;
Copper sulphate 0.02mg/L;
Cobalt chloride 0.05mg/L.
And the component of described organic reagent and the concentration of its correspondence as follows:
Thiamine hydrochloride 10.0mg/L;
Nicotinic acid 1.0mg/L;
Puridoxine hydrochloride 1.0mg/L;
Inositol 100.0mg/L.
The present invention utilizes the pear tree of the fast numerous seminal propagation difficulty of hot research plant somatocyte embryo generation technique in plant cell and tissue cultures to be in recent years only the fundamental way solved the problem, it not only can solve the problem that pear tree kind matter is degenerated, reach the object of good seed detoxification, purification and rejuvenation and Fast-propagation, and be the important channel of highly efficient regeneration seed source, still carry out the desirable acceptor material of genetic transformation or mutation breeding seed selection new varieties.
Below disclose the present invention with preferred embodiment, so it is not intended to limiting the invention, and all employings are equal to replacement or the technical scheme that obtains of equivalent transformation mode, all drop within protection scope of the present invention.
Claims (6)
1. a pear tree inductor embryogenesis medium, is characterized in that, comprises 1/2 improvement MS+0.5 ~ 1.0mgL
-16-BA+0.3 ~ 0.5mgL
-12,4-D+0.5 ~ 1.0mgL
-1nAA+6.0 ~ 7.0gL
-1agar+30gL
-1sucrose+0.1 ~ 0.3%phytagel, and pH value is 5.8 ~ 6.0.
2. a pear tree inductor embryogenesis medium, is characterized in that, comprises 1/2 improvement MS+0.8mgL
-16-BA+0.4mgL
-12,4-D+0.8mgL
-1nAA+6.5gL
-1agar+30gL
-1sucrose+0.2%phytagel, and pH value is 6.0.
3. a kind of pear tree inductor embryogenesis medium according to claim 1 and 2, it is characterized in that, described improvement MS comprises grand nutrition element, micronutrient element and organic reagent.
4. a kind of pear tree inductor embryogenesis medium according to claim 3, is characterized in that, the component of described grand nutrition element is as follows with the concentration of its correspondence:
Potassium nitrate 1900mg/L;
Ammonium sulfate 1500mg/L;
Epsom salt 350mg/L;
Calcium chloride dihydrate 420mg/L;
Anhydrous potassium dihydrogenphosphate 185mg/L;
Disodium ethylene diamine tetraacetate 26.8mg/L;
Ferrous sulfate heptahydrate 15.9mg/L.
5. a kind of pear tree inductor embryogenesis medium according to claim 3, is characterized in that, the component of described micronutrient element is as follows with the concentration of its correspondence:
Four water manganese sulphate 22.5mg/L;
Zinc sulphate 6.8mg/L;
Boric acid 5.4mg/L;
Potassium iodide 1.0mg/L;
Sodium molybdate 0.2mg/L;
Copper sulphate 0.02mg/L;
Cobalt chloride 0.05mg/L.
6. a kind of pear tree inductor embryogenesis medium according to claim 3, is characterized in that, the component of described organic reagent is as follows with the concentration of its correspondence:
Thiamine hydrochloride 10.0mg/L;
Nicotinic acid 1.0mg/L;
Puridoxine hydrochloride 1.0mg/L;
Inositol 100.0mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510938585.6A CN105379625A (en) | 2015-12-16 | 2015-12-16 | Pear inducer embryo formation medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510938585.6A CN105379625A (en) | 2015-12-16 | 2015-12-16 | Pear inducer embryo formation medium |
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| Publication Number | Publication Date |
|---|---|
| CN105379625A true CN105379625A (en) | 2016-03-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510938585.6A Pending CN105379625A (en) | 2015-12-16 | 2015-12-16 | Pear inducer embryo formation medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107333654A (en) * | 2017-08-07 | 2017-11-10 | 镇江万山红遍农业园 | A kind of Multiplying culture method of pear tree body embryo |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102124954A (en) * | 2011-01-31 | 2011-07-20 | 江苏农林职业技术学院 | Induced rapid propagation culture medium for somatic embryos of leaves in vitro of photinia x frasery |
| CN102648698A (en) * | 2012-05-23 | 2012-08-29 | 南京农业大学 | Pyrus stem tip tissue culture rapid propagation method |
| CN103444522A (en) * | 2012-06-04 | 2013-12-18 | 华中农业大学 | Preparation method for rooting of test-tube plantlet of ample flow pear |
-
2015
- 2015-12-16 CN CN201510938585.6A patent/CN105379625A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102124954A (en) * | 2011-01-31 | 2011-07-20 | 江苏农林职业技术学院 | Induced rapid propagation culture medium for somatic embryos of leaves in vitro of photinia x frasery |
| CN102648698A (en) * | 2012-05-23 | 2012-08-29 | 南京农业大学 | Pyrus stem tip tissue culture rapid propagation method |
| CN103444522A (en) * | 2012-06-04 | 2013-12-18 | 华中农业大学 | Preparation method for rooting of test-tube plantlet of ample flow pear |
Non-Patent Citations (1)
| Title |
|---|
| 薛光荣等: "锦丰梨花粉植株的诱导", 《园艺学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107333654A (en) * | 2017-08-07 | 2017-11-10 | 镇江万山红遍农业园 | A kind of Multiplying culture method of pear tree body embryo |
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Application publication date: 20160309 |