CN105548299B - Method and device for rapidly detecting acetochlor residues in soil - Google Patents
Method and device for rapidly detecting acetochlor residues in soil Download PDFInfo
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- CN105548299B CN105548299B CN201510996149.4A CN201510996149A CN105548299B CN 105548299 B CN105548299 B CN 105548299B CN 201510996149 A CN201510996149 A CN 201510996149A CN 105548299 B CN105548299 B CN 105548299B
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- VTNQPKFIQCLBDU-UHFFFAOYSA-N Acetochlor Chemical group CCOCN(C(=O)CCl)C1=C(C)C=CC=C1CC VTNQPKFIQCLBDU-UHFFFAOYSA-N 0.000 title claims abstract description 73
- 239000002689 soil Substances 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000001514 detection method Methods 0.000 claims abstract description 19
- 239000003792 electrolyte Substances 0.000 claims abstract description 13
- 229910052736 halogen Inorganic materials 0.000 claims abstract description 12
- 150000002367 halogens Chemical class 0.000 claims abstract description 12
- 238000005513 bias potential Methods 0.000 claims abstract description 7
- 238000012360 testing method Methods 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims description 34
- 108010015776 Glucose oxidase Proteins 0.000 claims description 26
- 239000004366 Glucose oxidase Substances 0.000 claims description 26
- 229940116332 glucose oxidase Drugs 0.000 claims description 26
- 235000019420 glucose oxidase Nutrition 0.000 claims description 26
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 23
- 229910021389 graphene Inorganic materials 0.000 claims description 23
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 16
- 229910052799 carbon Inorganic materials 0.000 claims description 15
- 229920001661 Chitosan Polymers 0.000 claims description 13
- 238000005259 measurement Methods 0.000 claims description 12
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical class Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 claims description 8
- 229910052697 platinum Inorganic materials 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 8
- 239000008055 phosphate buffer solution Substances 0.000 claims description 6
- 230000005611 electricity Effects 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000001548 drop coating Methods 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 229910021397 glassy carbon Inorganic materials 0.000 claims description 4
- 230000001629 suppression Effects 0.000 claims description 4
- 229940075397 calomel Drugs 0.000 claims description 3
- 238000004090 dissolution Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- 150000004676 glycans Chemical class 0.000 claims 1
- 229910002804 graphite Inorganic materials 0.000 claims 1
- 239000010439 graphite Substances 0.000 claims 1
- -1 graphite Alkene Chemical class 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 claims 1
- 238000005070 sampling Methods 0.000 abstract description 2
- 230000001678 irradiating effect Effects 0.000 abstract 1
- 238000011049 filling Methods 0.000 description 6
- 239000002953 phosphate buffered saline Substances 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical class [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 3
- 238000002848 electrochemical method Methods 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000003760 magnetic stirring Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000012490 blank solution Substances 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 150000001721 carbon Chemical class 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000000835 electrochemical detection Methods 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 231100000025 genetic toxicology Toxicity 0.000 description 1
- 230000001738 genotoxic effect Effects 0.000 description 1
- 125000003051 glycosyloxy group Chemical group 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000004060 quinone imines Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3273—Devices therefor, e.g. test element readers, circuitry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/36—Glass electrodes
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
A method for rapidly detecting acetochlor residues in soil comprises the following steps: a. a linear plot of acetochlor concentration versus current was made. Respectively putting a working electrode, a reference electrode and an auxiliary electrode into an electrolytic cell; a halogen lamp light source is arranged below the electrolytic cell; under the condition that the bias potential is 0V, irradiating the acetochlor electrolyte from the lower direction of the electrolytic cell by using halogen lamp light sources, and respectively measuring the photocurrent signal values of the acetochlor solutions with different known concentrations; a linear plot of acetochlor concentration versus suppressed photocurrent was prepared. b. Measuring the photocurrent signal value of the acetochlor solution to be tested, and calculating the concentration of the acetochlor in the test sample. A device for rapidly detecting acetochlor residue in soil comprises an electrochemical workstation, a light source, an electrolytic cell, a working electrode, a reference electrode and an auxiliary electrode. The invention has the advantages of low detection cost, high automation degree, simple and convenient operation, small sampling amount and short time consumption.
Description
Technical field
The present invention relates to one kind to be based on electrochemical method, more particularly to a kind of side of quick detection acetochlor in soil residual
Method.
Background technology
Acetochlor is one of maximum dry land herbicide of currently used area, but because Acetochlor degradation cycle is longer,
It is easy to migrate in water body and soil environment, and its metabolite quinone imines has weak genotoxicity and carcinogenesis.
Before making the present invention, the method for conventional detection Acetochlor is mostly that chromatography is combined with other technologies.Using mass spectrum
Method can improve sensitivity and the selectivity of analysis, but instrument is higher using more complicated and cost, sample extraction purifying step phase
To cumbersome, sensitivity is influenceed by steps such as sample purification, concentrations, and these methods require higher to instrument and equipment.
The content of the invention
The purpose of the present invention, which is that, overcomes drawbacks described above, develops the method that Acetochlor is quickly determined based on electrochemical method
And detection means, testing cost is low, automaticity is high, easy to operate and sampling amount is small, takes short.
First purpose of the present invention is achieved through the following technical solutions, and a kind of quick detection acetochlor in soil is residual
The method stayed, comprises the following steps：
A, the linear relationship chart of Acetochlor concentration and electric current is made：
（1）Cleaning glassy carbon electrode surface with after graphene/glucose oxidase/chitosan suspension drop coating, be placed in 4
Dried under °C, be made working electrode, be i.e. the glass-carbon electrode of graphene/glucose oxidase/chitosan-modified；
（2）The phosphate buffer solution that 10mL contains 0.8mM glucose is added in electrolytic cell, then sequentially adds difference
The Acetochlor solution of concentration known is into electrolytic cell；
（3）Using the glass-carbon electrode of graphene/glucose oxidase/chitosan-modified as working electrode, with saturation calomel electricity
Extremely reference electrode, using platinum electrode as auxiliary electrode, working electrode, reference electrode and auxiliary electrode are respectively put into electrolytic cell
In；Halogen light light source is set below electrolytic cell；Under conditions of bias potential is 0V, halogen light light source is from below electrolytic cell
Acetochlor electrolyte is irradiated upwards, measurement respectively obtains the photo-signal of the Acetochlor solution of different concentration knowns
Value；
（4）Using the concentration of Acetochlor solution as abscissa, using the suppression photo-signal value of Acetochlor solution as ordinate,
Acetochlor concentration is made with suppressing the linear relationship chart of photoelectric current；
B, Acetochlor concentration in test sample：
（1）Cleaning glassy carbon electrode surface with after graphene/glucose oxidase/chitosan suspension drop coating, be placed in 4
°C dry, the glass-carbon electrode of graphene/glucose oxidase/chitosan-modified is made；
（2）The Acetochlor solution to be measured for extracting to obtain from soil is quantitatively adding and contains 0.8mM glucose equipped with 10mL
In the electrolytic cell of phosphate buffer solution, Acetochlor electrolyte to be measured is formed；
（3）Using the glass-carbon electrode of graphene/glucose oxidase/chitosan-modified as working electrode, with saturation calomel electricity
Extremely reference electrode, using platinum electrode as auxiliary electrode, working electrode, reference electrode and auxiliary electrode are respectively put into second grass to be measured
In amine electrolyte；Halogen light light source is set below electrolytic cell；Under conditions of bias potential is 0V, halogen light light source is certainly electric
Acetochlor electrolyte to be measured is irradiated upwards below Xie Chi, measurement obtains the photo-signal of Acetochlor solution to be measured
Value；
（4）Using linear relationship chart of the Acetochlor concentration made from suppressing photoelectric current, second grass to be measured is obtained with measurement
The suppression photo-signal value of amine aqueous solution, calculates Acetochlor concentration in test sample.
Preferably, pedotheque acetonitrile dissolution filter.
Preferably, the halogen light light source is 250 watts.
Preferably, the step a（2）Described in the concentration of Acetochlor solution of different concentration knowns be at least three.
Preferably, the electrolytic cell material is glass.
Preferably, the graphene/glucose oxidase/chitosan suspension collocation method：0.5g Chitosan powder
Add in 100mL 1% acetum, 0.5% chitosan solution is made；1.0mg graphene and 0.6mg grape glycosyloxy
Change in enzyme addition 1.0mL 0.5% chitosan solution and graphene/glucose oxidase/chitosan suspension is made.
Second object of the present invention is achieved through the following technical solutions, and a kind of quick detection acetochlor in soil is residual
The device stayed, including electrochemical workstation, light source, electrolytic cell, working electrode, reference electrode and auxiliary electrode, by working electrode,
Reference electrode and auxiliary electrode are made three-electrode system, and working electrode is the glass of graphene/glucose oxidase/chitosan-modified
Carbon electrode, reference electrode are saturated calomel electrode, and auxiliary electrode is platinum electrode；Working electrode, reference electrode are set in electrolytic cell
And auxiliary electrode；Light source is set below electrolytic cell；Electrochemical workstation includes Survey Software and three electrode interfaces, electrode interface point
Lian Jie not working electrode, reference electrode and auxiliary electrode.
Compared with prior art, the invention has the advantages that：
First, advantages of the present invention and effect are sample treatment simple possible, are dissolved with acetonitrile；Instrument performance is steady
Fixed, reliable results are easy to operate, and automaticity is high, as a result directly displays, is not required to professional；
Second, take short, 3min can obtain analysis result, and testing cost is low, automaticity is high, easy to operate and take
Sample amount is small, the detection for the acetochlor in soil content that is particularly suitable for use in, and directly soil can be taken to be analyzed as former state, before complicated
Processing procedure.
Brief description of the drawings
Fig. 1 is the glass-carbon electrode of graphene/glucose oxidase of the present invention/chitosan-modified to being separately added into known to difference
The Acetochlor solution of concentration is in the photocurrent response figure of the phosphate buffer solution containing 0.8mM glucose（CK is blank solution,
1-5 concentration increases successively）；
Fig. 2 is linear relationship chart of the Acetochlor concentration of the present invention with suppressing photoelectric current；
Fig. 3 is the structural representation of the device of quick detection acetochlor in soil residual of the present invention；
In figure：1. auxiliary electrode, 2. working electrodes（The glass-carbon electrode of graphene/glucose oxidase/chitosan-modified）、
3. reference electrode, 4. saturated potassium chloride solutions, 5. electrolytic cells, 6.PBS solution.
Embodiment
A kind of method of quick detection acetochlor in soil residual, its major technique step include：
（1）Phosphate buffer solution is added in electrolytic cell；
（2）Glucose solution is added in the electrolytic cell；
（3）Start magnetic stirring apparatus；
（4）Bias potential is set；
（5）Working electrode, reference electrode, auxiliary electrode are put into electrolytic cell, Acetochlor solution is added in electrolytic cell,
Magnetic stirring apparatus is closed after 30 seconds.
（6）Measure photo-signal.
Working electrode is that graphene/glucose oxidase/chitosan-modified glass-carbon electrode is working electrode, the reference
Electrode is saturated calomel electrode, and the auxiliary electrode is platinum electrode.
The PBS solution 6 that 10mL contains 0.8mM glucose is added in electrolytic cell 5（PBS is phosphate buffer solution）, so
Working electrode 2, reference electrode 3, auxiliary electrode 1 are inserted in electrolytic cell 5 afterwards.Acetochlor solution is added in electrolytic cell again, made
Acetochlor suppresses the activity of the glucose oxidase on working electrode, and photo-signal is determined in electrolytic cell.Acetochlor contains
Amount is inversely proportional to the photo-signal intensity of output, and manufactured instrument is self-reacting device, and one-shot measurement takes about 3min, and reagent is used
Amount can be controlled in 10 μ L.Three-electrode system insertion electrolytic cell especially is made in working electrode, reference electrode and auxiliary electrode
In detected, so as to both ensureing that Acetochlor fully suppresses glucose oxidase activity in sample, ensure few sample size again,
With less sample so when carrying out Acetochlor detection, so as to also ensure not pollute environment.
Electrolyte is neutral electrolyte, after glucose solution is added, directly can measure Acetochlor with apparatus of the present invention.
Electrolyte selects neutral phosphate buffered saline solution.
Based on the measurement apparatus of electrochemical method determining Acetochlor property, including electrochemical workstation, light source, electrolytic cell 5,
Working electrode 2, reference electrode 3 and auxiliary electrode 1 are formed, it is characterized in that working electrode, reference electrode and auxiliary electrode are made
Three-electrode system, working electrode are the glass-carbon electrode of graphene/glucose oxidase/chitosan-modified, can be adjusted as needed
Electrode size, guarantee to insert smaller electrolytic cell with this or carry out on-line checking, reference electrode is saturated calomel electrode, auxiliary
It is platinum electrode to help electrode.Reference electrode is placed in internal-filling liquid, and internal-filling liquid is conventional saturated potassium chloride solution 4 etc..
Electrochemical workstation includes Survey Software and three electrode interfaces, and interface connects different electrodes, green connection respectively
Working electrode, white connection reference electrode, red connection auxiliary electrode.Electrolyte selects neutral PBS solution.
In measurement apparatus of the present invention, the three-electrode system that working electrode, reference electrode and auxiliary electrode are formed inserts electrolysis
Chi Zhong.Electrolytic cell uses glass, and working electrode is located on megohmite insulant；Reference electrode is placed in internal-filling liquid, and internal-filling liquid is conventional
Saturated potassium chloride solution, ensure conductive between electrode internal-filling liquid and external solution and internal-filling liquid does not ooze out；Working electrode diameter
, it is necessary to more samples and bigger electrolytic cell when big, but the present invention can be as needed using the glass-carbon electrode of different area, can
It can be controlled in 10 μ L with less reagent, such as reagent dosage.The reagent is the dosage that Acetochlor measures every time.
During measurement, three electrode new systems are all inserted into the electrolyte for the PBS that 10mL contains 0.8mM glucose, are being electrolysed
Acetochlor solution is added in pond, the glucose oxidase activity of working electrode surface is suppressed, then bias potential is set, press
Lower measurement switch starts to measure, and photo-signal corresponding to Acetochlor, unit A can be obtained after 3min.Measurement terminates.Measure
Photo-signal.Monitoring lower-cut can as little as 10-12mol/L.The size of Acetochlor concentration has with the photo-signal intensity exported
Close, shown so as to be calculated result by the setting of instrument in itself（It is automatically performed by instrument）.
Claims (7)
- A kind of 1. method of quick detection acetochlor in soil residual, it is characterised in that the detection method comprises the following steps：A, the linear relationship chart of Acetochlor concentration and electric current is made：（1）Cleaning glassy carbon electrode surface with after graphene/glucose oxidase/chitosan suspension drop coating, be placed in 4 °C Under dry, be made working electrode, i.e. the glass-carbon electrode of graphene/glucose oxidase/chitosan-modified；（2）The phosphate buffer solution that 10mL contains 0.8mM glucose is added in electrolytic cell, is then sequentially added known to difference The Acetochlor solution of concentration is into electrolytic cell；（3）Using the glass-carbon electrode of graphene/glucose oxidase/chitosan-modified as working electrode, using saturated calomel electrode as Reference electrode, using platinum electrode as auxiliary electrode, working electrode, reference electrode and auxiliary electrode are respectively put into electrolytic cell；Electricity Halogen light light source is set below Xie Chi；Under conditions of bias potential is 0V, halogen light light source is upward from below electrolytic cell Acetochlor electrolyte is irradiated, measurement respectively obtains the photo-signal value of the Acetochlor solution of different concentration knowns；（4）Using the concentration of Acetochlor solution as abscissa, using the suppression photo-signal value of Acetochlor solution as ordinate, make Acetochlor concentration and the linear relationship chart for suppressing photoelectric current；B, Acetochlor concentration in test sample：（1）Cleaning glassy carbon electrode surface with after graphene/glucose oxidase/chitosan suspension drop coating, be placed in 4 °C Dry, the glass-carbon electrode of graphene/glucose oxidase/chitosan-modified is made；（2）The Acetochlor solution to be measured for extracting to obtain from soil is quantitatively adding to the phosphorus for containing 0.8mM glucose equipped with 10mL In the electrolytic cell of hydrochlorate cushioning liquid, Acetochlor electrolyte to be measured is formed；（3）Using the glass-carbon electrode of graphene/glucose oxidase/chitosan-modified as working electrode, using saturated calomel electrode as Reference electrode, using platinum electrode as auxiliary electrode, working electrode, reference electrode and auxiliary electrode are respectively put into Acetochlor electricity to be measured Solve in liquid；Halogen light light source is set below electrolytic cell；Under conditions of bias potential is 0V, halogen light light source is from electrolytic cell Lower section is irradiated to Acetochlor electrolyte to be measured upwards, and measurement obtains the photo-signal value of Acetochlor solution to be measured；（4）Using linear relationship chart of the Acetochlor concentration made from suppressing photoelectric current, it is molten that Acetochlor to be measured is obtained with measurement The suppression photo-signal value of liquid, calculates Acetochlor concentration in test sample.
- 2. the method for quick detection acetochlor in soil residual according to claim 1, it is characterised in that the soil-like Product acetonitrile dissolution filter.
- 3. the method for quick detection acetochlor in soil residual according to claim 1, it is characterised in that the Halogen lamp LED Radiant is 250 watts.
- 4. the method for quick detection acetochlor in soil residual according to claim 1, it is characterised in that the step a （2）Described in the concentration of Acetochlor solution of different concentration knowns be at least three.
- 5. the method for quick detection acetochlor in soil residual according to claim 1, it is characterised in that the electrolytic cell Material is glass.
- 6. the method for quick detection acetochlor in soil residual according to claim 1, it is characterised in that the graphite Alkene/glucose oxidase/chitosan suspension collocation method：0.5g Chitosan powder adds 100mL 1% acetum In, be made 0.5% chitosan solution；1.0mg graphene and 0.6mg glucose oxidase add 1.0mL 0.5% shell Graphene/glucose oxidase/chitosan suspension is made in glycan solution.
- 7. a kind of device of quick detection acetochlor in soil residual, it is characterized in that, described device includes electrochemical workstation, light Source, electrolytic cell, working electrode, reference electrode and auxiliary electrode, three electrodes are made in working electrode, reference electrode and auxiliary electrode System, working electrode are the glass-carbon electrode of graphene/glucose oxidase/chitosan-modified, and reference electrode is saturation calomel electricity Pole, auxiliary electrode are platinum electrode；Working electrode, reference electrode and auxiliary electrode are set in electrolytic cell；Light is set below electrolytic cell Source；Electrochemical workstation includes Survey Software and three electrode interfaces, and electrode interface connects working electrode, reference electrode and auxiliary respectively Help electrode.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297151A (en) * | 1999-12-22 | 2001-05-30 | 中国人民解放军军事医学科学院放射医学研究所 | Method and special instrument for in-situ detection of organophosphorus agricultural chemical |
| CN103743797A (en) * | 2014-01-02 | 2014-04-23 | 青岛大学 | Novel method for assembling multi-layer biological enzyme sensor to improve detection performance by using graphene |
| CN104215674A (en) * | 2014-09-17 | 2014-12-17 | 扬州工业职业技术学院 | Method for rapidly detecting clethodim pesticide residues in soil |
| CN104877985A (en) * | 2015-04-14 | 2015-09-02 | 上海大学 | Preparation method of carrier capable of improving thermal stability of glucose oxidase based on graphene |
-
2015
- 2015-12-28 CN CN201510996149.4A patent/CN105548299B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297151A (en) * | 1999-12-22 | 2001-05-30 | 中国人民解放军军事医学科学院放射医学研究所 | Method and special instrument for in-situ detection of organophosphorus agricultural chemical |
| CN103743797A (en) * | 2014-01-02 | 2014-04-23 | 青岛大学 | Novel method for assembling multi-layer biological enzyme sensor to improve detection performance by using graphene |
| CN104215674A (en) * | 2014-09-17 | 2014-12-17 | 扬州工业职业技术学院 | Method for rapidly detecting clethodim pesticide residues in soil |
| CN104877985A (en) * | 2015-04-14 | 2015-09-02 | 上海大学 | Preparation method of carrier capable of improving thermal stability of glucose oxidase based on graphene |
Non-Patent Citations (2)
| Title |
|---|
| iosensor for atrazin based on aligned carbon nanotubes modified with glucose oxidase;Qing Yang et al.;《Microchim Acta》;20101231;Square wave voltammetry (SWV) of GOx-aligned CNTs –Cu electrode、Determination of atrazine * |
| Visible-light-activated photoelectrochemical biosensor for the study of acetylcholinesterase inhibition induced by endogenous neurotoxins;Qilin Huang et al.;《BiosensorsandBioelectronics》;20130214;第45卷;全文 * |
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