CN105543097B - A kind of collection method of active microalgae adsorbent material - Google Patents
A kind of collection method of active microalgae adsorbent material Download PDFInfo
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- CN105543097B CN105543097B CN201610007538.4A CN201610007538A CN105543097B CN 105543097 B CN105543097 B CN 105543097B CN 201610007538 A CN201610007538 A CN 201610007538A CN 105543097 B CN105543097 B CN 105543097B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- Water Treatment By Sorption (AREA)
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Abstract
The invention discloses a kind of collection methods of active microalgae adsorbent material, comprising the following steps: 1) changes the microalgae cell physiological metabolism period by regulating illumination intensity, control frustule floats regular；2) it after microalgae cell floats, realizes and is separated by solid-liquid separation using vacuum pump and double-layer leaching net filtering, collect microalgae cell；The present invention is 8000-10000lux by manual adjustment illumination condition, irradiation microalgae cell 1-2.5 hours, after microalgae cell sufficiently floats, vacuum pump suction is controlled in underwater 0.5-1.5cm, 80-200 mesh filter screen is selected to realize the collection of microalgae cell by vacuum pump and double-layer leaching net filtering, the recycling of the microalgae cells such as achievable microcystic aeruginosa, chlamydomonas, the Anabaena spiroides in processing heavy metal wastewater thereby, organic polluting water of the present invention, the rate of recovery is up to 80%-85%；Collection method step of the invention is simple, at low cost, and collection efficiency is high, is worth being widely popularized.
Description
Technical field
The present invention relates to field of environment engineering, the collection method of specifically a kind of active microalgae adsorbent material, by artificial
Illumination condition is adjusted, the physiological metabolism cycle of activity of microalgae is changed, its floating in sewage is controlled and sinks rule, be microalgae material
The collection of material creates conditions.
Background technique
Biosorption process is a kind of emerging water treatment technology, and biosorption technology is applied to water treatment field, is such as located
It has broad prospects in terms of reason organic polluting water, heavy metal wastewater thereby；Biological adsorption refers to and utilizes various natural organisms
Or derivatives thereof absorption pollutant soluble in water, then be separated by solid-liquid separation, reach water body purification purpose；Biological adsorption material source
It is abundant, processing cost can be significantly reduced；Relative to general biological adsorption agent, microalgae can voluntarily be increased by photosynthesis
Reproductive growth, it is only necessary to which a small amount of inorganic nutriment is provided；More importantly the microalgae of some types can be regulated and controled by illumination and temperature,
It swims on the water surface, it is convenient to collect, and can substantially reduce the cost of adsorbent material removal process, this is other biological adsorption agent institutes
The advantage not having.
Summary of the invention
The purpose of the present invention is to provide a kind of collections of easy to operate, efficient, inexpensive active microalgae adsorbent material
Method, to solve the problems mentioned in the above background technology.
To achieve the above object, the invention provides the following technical scheme:
A kind of collection method of active microalgae adsorbent material, comprising the following steps:
1) the microalgae cell physiological metabolism period is changed by regulating illumination intensity, control frustule floats regular；
2) it after microalgae cell floats, realizes and is separated by solid-liquid separation using vacuum pump and double-layer leaching net filtering, collect microalgae cell.
As a further solution of the present invention: in the step 1 after purification absorption, strengthening the light that microalgae cell floats
Strong condition is 8000-10000lux, is 2-4 times of normal sorption process, and irradiation time is 1-2.5 hours.
As further scheme of the invention: the step 2 intermediate pump suction nozzle is controlled in underwater 0.5-1.5cm,
Select 80-200 mesh filter screen.
As further scheme of the invention: the collection method of active microalgae adsorbent material of the invention, for collecting
Handle heavy metal wastewater thereby, the microcystic aeruginosa in organic polluting water, chlamydomonas, Anabaena spiroides；The rate of recovery of microalgae cell is
80%-85%。
Compared with prior art, the beneficial effects of the present invention are: the present invention is high using the physiological metabolism period of microalgae cell
Degree relies on the characteristics of intensity of illumination, after completing pollutant absorption, by increasing intensity of illumination, microalgae cell is promoted to float on water
Face separates microalgae cell by circulating filtration, by this collection method, Cu in waste water is green micro- using special double-layer leaching net
The rate of recovery of the microalgae cells such as capsule algae, chlamydomonas, Anabaena spiroides can reach 80%-85%.
Specific embodiment
The technical solution of the patent is explained in further detail With reference to embodiment.
Embodiment 1
A kind of collection method of active microalgae adsorbent material, comprising the following steps:
1) light intensity of microcystic aeruginosa Water warfare cupric (< 10mg/L) is 4500lux, at the end of purification process to be adsorbed,
Light intensity is increased to 9500lux, frustule is promoted to float, irradiation time is 2.5 hours；
2) it after microalgae cell sufficiently floats, realizes and is separated by solid-liquid separation using vacuum pump and double-layer leaching net filtering, it is thin to collect microalgae
Born of the same parents；The control of vacuum pump suction selects 150 mesh filter screens in 0.8 cm of underwater, bilateral strainer.
The collection method of active microalgae adsorbent material of the invention, for collecting Treatment of Copper waste water Microcystis aeruginosa Strains
The rate of recovery be 81 ± 3%.
Embodiment 2
A kind of collection method of active microalgae adsorbent material, comprising the following steps:
1) microcystic aeruginosa Water warfare contains the light intensity of benzene (< 8mg/L) for 3800lux, at the end of purification process to be adsorbed, incites somebody to action
Light intensity is increased to 9500lux, and frustule is promoted to float, and irradiation time is 2 hours；
2) it after microalgae cell sufficiently floats, realizes and is separated by solid-liquid separation using vacuum pump and double-layer leaching net filtering, it is thin to collect microalgae
Born of the same parents；The control of vacuum pump suction selects 150 mesh filter screens in 0.8 cm of underwater, bilateral strainer.
The collection method of active microalgae adsorbent material of the invention, for collecting Treatment of Copper waste water Microcystis aeruginosa Strains
The rate of recovery be 83 ± 2%.
Embodiment 3
A kind of collection method of active microalgae adsorbent material, comprising the following steps:
1) chlamydomonas Water warfare contains the light intensity of zinc (< 15mg/L) for 3000lux, at the end of purification process to be adsorbed, by light intensity
It is increased to 8500lux, frustule is promoted to float, irradiation time is 1.8 hours；
2) it after microalgae cell sufficiently floats, realizes and is separated by solid-liquid separation using vacuum pump and double-layer leaching net filtering, it is thin to collect microalgae
Born of the same parents；The control of vacuum pump suction selects 100 mesh filter screens in 1.5 cm of underwater, bilateral strainer.
The collection method of active microalgae adsorbent material of the invention, for collecting returning for Treatment of Zinc-containing Wastewater copper reinhardtii cell
Yield is 85 ± 3%.
Embodiment 4
A kind of collection method of active microalgae adsorbent material, comprising the following steps:
1) light intensity of Anabaena spiroides Water warfare cupric (< 10mg/L) and benzene (< 5mg/L) is 5000lux, purification to be adsorbed
At the end of process, light intensity is increased to 10000lux, frustule is promoted to float, irradiation time is 2.5 hours；
2) it after microalgae cell sufficiently floats, realizes and is separated by solid-liquid separation using vacuum pump and double-layer leaching net filtering, it is thin to collect microalgae
Born of the same parents；The control of vacuum pump suction selects 80 mesh filter screens in underwater 1.0cm, bilateral strainer.
The collection method of active microalgae adsorbent material of the invention, for collecting Treatment of Copper and benzene waste water anabena cell
The rate of recovery be 82 ± 4%.
The preferred embodiment of the patent is described in detail above, but this patent is not limited to above-mentioned embodiment party
Formula within the knowledge of one of ordinary skill in the art can also be under the premise of not departing from this patent objective
Various changes can be made.
Claims (3)
1. a kind of collection method of active microalgae adsorbent material, which comprises the following steps:
1) the microalgae cell physiological metabolism period is changed by regulating illumination intensity, control frustule floats regular；
2) it after microalgae cell floats, realizes and is separated by solid-liquid separation using vacuum pump and double-layer leaching net filtering, collect microalgae cell；
In the step 1) after purification absorption, strengthening the light-intensity conditions that microalgae cell floats is 8500-10000lux, irradiation
Time is 1.8-2.5 hours.
2. the collection method of active microalgae adsorbent material according to claim 1, which is characterized in that step 2 intermediate pump
Suction nozzle control selects 80-200 mesh filter screen in underwater 0.5-1.5cm.
3. the collection method of active microalgae adsorbent material according to claim 1, which is characterized in that for collecting processing weight
Microcystic aeruginosa, chlamydomonas, Anabaena spiroides in metallic wastewater, organic polluting water；The rate of recovery of microalgae cell is 80%-
85%。
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| CN201610007538.4A CN105543097B (en) | 2016-01-07 | 2016-01-07 | A kind of collection method of active microalgae adsorbent material |
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| CN201610007538.4A CN105543097B (en) | 2016-01-07 | 2016-01-07 | A kind of collection method of active microalgae adsorbent material |
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| CN110320168B (en) * | 2019-07-31 | 2021-07-09 | 河海大学 | Method for measuring light transmittance inside microcystis colony |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774764A (en) * | 2015-04-03 | 2015-07-15 | 新奥科技发展有限公司 | Immobilized culture method and immobilized culture device for microalgae |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774764A (en) * | 2015-04-03 | 2015-07-15 | 新奥科技发展有限公司 | Immobilized culture method and immobilized culture device for microalgae |
Non-Patent Citations (2)
| Title |
|---|
| 光照对微藻水华的影响;施丰华等;《安徽农业科学》;20111231;第39卷(第16期);第9801页右栏第3段-第9802页左栏第2段 |
| 微藻生物质采收方法的经济性和效率研究进展;沈英等;《湖北农业科学》;20121130;第51卷(第22期);第4983页右栏第3段第4984页左栏第4段 |
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