CN105542028A - Water-soluble pea polysaccharide and preparation method thereof - Google Patents
Water-soluble pea polysaccharide and preparation method thereof Download PDFInfo
- Publication number
- CN105542028A CN105542028A CN201610099969.8A CN201610099969A CN105542028A CN 105542028 A CN105542028 A CN 105542028A CN 201610099969 A CN201610099969 A CN 201610099969A CN 105542028 A CN105542028 A CN 105542028A
- Authority
- CN
- China
- Prior art keywords
- water
- pea
- soluble
- preparation
- polysaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
Abstract
The invention belongs to the technical field of food additives, and discloses a water-soluble pea polysaccharide and a preparation method thereof. The preparation method comprises the steps that dried pea dregs are taken and dissolved for 0.5-1 h by adding deionized water, and centrifuging is performed after the pH of the system is regulated to be 8.0-10.0 with alkali; supernatant liquor is removed, 10-30 times of deionized water is added into precipitates, and the pH of the system is regulated to be 4.0-7.0 with acid; steaming is performed for 0.5-3 hours at the temperature of 105 DEG C-125 DEG C, the temperature is lowered to room temperature, centrifuging and concentrating are performed to obtain water-soluble pea polysaccharide concentration liquor, drying is performed, and then the water-soluble pea polysaccharide is obtained. The water-soluble pea polysaccharide has the higher tolerance to temperature, pH, ionic concentration and the like, has the viscosity which is far lower than that of other stabilizing agents and has the good application prospect by serving as a food additive.
Description
Technical field
The invention belongs to technical field of food additives, be specifically related to a kind of water-soluble pea polysaccharide and preparation method thereof.
Background technology
Pea is the fourth-largest legume crop in the world.China, as second-biggest-in-the-world pea producing country, has abundant pea resource.Current pea mainly for the production of starch and vermicelli product, such as Longkou bean vermicelli.The byproduct after starch and albumen is extracted with pea, i.e. pea slag (pea fibre), annual have a large amount of processing byproducts to be abandoned by factory or be used as animal-feed, seldom has producer to carry out being recycled of bean dregs, cause great environmental pollution and the wasting of resources.We extract pea polysaccharide from pea slag, greatly can improve the added value of China's bean dregs and the efficiency utilization to pea.
Soluble polysaccharide is the study hotspot of current food service industry, most of polysaccharide all have hypotensive, reduce serum cholesterol, the functional performance such as physiologically active and stably dispersing, emulsification and film forming such as anti-oxidant.Research shows, pea polysaccharide has the effects such as anti-oxidant, hypoglycemic.
Soybean polysaccharide and pectin have good dispersion stabilization all respectively.Pectin mainly makes it stablize by the viscosity improving protein drinks, adds the drink that soybean polysaccharide then can obtain lower thickness sense.In China, yogurt drink is deeply by the favor of consumers in general, utilize the stable emulsifying characteristic of water-soluble pea polysaccharide can form the product of acid light mouthfeel, food fibre can be provided to supplement simultaneously, without the need to using with other compound stabilizers, this is with a wide range of applications in yogurt drink industry.Galacturonic acid content in current discovery pea polysaccharide is about 17%, and structure proximate is in soybean soluble polysaccharide.
Summary of the invention
Based on above prior art, primary and foremost purpose of the present invention is the preparation method providing a kind of water-soluble pea polysaccharide.The method with pea slag for raw material prepare high dispersion stability can foodstuff additive-water-soluble pea polysaccharide.
Another object of the present invention is to provide a kind of water-soluble pea polysaccharide prepared by aforesaid method.
The object of the invention is achieved through the following technical solutions:
A preparation method for water-soluble pea polysaccharide, comprises following preparation process:
Get the dry bean dregs of pea, add deionized water, dissolving 0.5 ~ 1h, is centrifugal after 8.0 ~ 10.0 with alkali regulation system pH, removing supernatant liquor, the deionized water of 10 ~ 30 times of quality is added in precipitation, be 4.0 ~ 7.0 with sour regulation system pH, at 105 ~ 125 DEG C, boiling was cooled to room temperature after 0.5 ~ 3 hour, then obtained pea soluble polysaccharide concentrated solution through centrifugal, concentrated, drying, obtains water-soluble pea polysaccharide.
The dry bean dregs of described pea refer to the waste pea slag (pea fibre) after pea extraction starch and albumen.
Preferably, described precipitation is 1:(20 ~ 30 with the mass ratio of the deionized water added).
Preferably, described alkali refers to sodium hydroxide, and described acid refers to hydrochloric acid.
Preferably, the sour regulation system pH of described use is 5.0 ~ 6.0.
Preferably, described boiling temperature is 110 ~ 120 DEG C, and cooking time is 1.5 ~ 2h.
Preferably, described drying refers to spraying dry, warm air drying or vacuum lyophilization.
A kind of water-soluble pea polysaccharide, is prepared by above method.
Preparation method of the present invention and the product tool obtained have the following advantages and beneficial effect:
(1) the water-soluble pea polysaccharide that prepared by the present invention has higher tolerance to temperature, pH and salt ionic concentration etc., and viscosity is well below other stablizer, such as pectin;
(2) the present invention utilize pea slag for raw material extracts water-soluble pea polysaccharide, turn waste into wealth, not only alleviate environmental pollution, and greatly improve the utility value of pea resource;
(3) the present invention adopts the method for high-temp extracting under slightly acidic condition, and gained water-soluble pea polysaccharide yield is higher and dispersion stabilization is better.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
The abundant stirring and dissolving 0.5h of appropriate amount of deionized water is added in waste pea slag after extracting starch and from albumen to pea, with (7500rpm centrifugal after sodium hydroxide regulation system pH8.0,25min, 4 DEG C), abandoning supernatant is to remove deproteinize, residue precipitation adds the deionized water of 10 times of quality, with hydrochloric acid regulation system pH4.0 under fully stirring, and room temperature is cooled to after boiling 2h at 125 DEG C, then centrifugation (10000rpm, 30min, 4 DEG C) obtain supernatant liquor and throw out.Supernatant concentration is obtained pea soluble polysaccharide concentrated solution, then namely lyophilize obtains water-soluble pea polysaccharide, and yield is 16.03%.The k value that the aqueous solution being configured to 10% records at 20 DEG C is 103mPas.
Embodiment 2
The abundant stirring and dissolving 1h of appropriate amount of deionized water is added in waste pea slag after extracting starch and from albumen to pea, with (7500rpm centrifugal after sodium hydroxide regulation system pH10.0,25min, 4 DEG C), abandoning supernatant is to remove deproteinize, residue precipitation adds the deionized water of 15 times of quality, with hydrochloric acid regulation system pH4.5 under fully stirring, and room temperature is cooled to after boiling 1.5h at 120 DEG C, then centrifugation (10000rpm, 30min, 4 DEG C) obtain supernatant liquor and throw out.Supernatant concentration is obtained pea soluble polysaccharide concentrated solution, then namely spraying dry obtains water-soluble pea polysaccharide, and yield is 12.94%.The k value that the aqueous solution being configured to 10% records at 20 DEG C is 111mPas.
Embodiment 3
The abundant stirring and dissolving 1h of appropriate amount of deionized water is added in waste pea slag after extracting starch and from albumen to pea, with (7500rpm centrifugal after sodium hydroxide regulation system pH9.0,25min, 4 DEG C), abandoning supernatant is to remove deproteinize, residue precipitation adds the deionized water of 20 times of quality, with hydrochloric acid regulation system pH7.0 under fully stirring, and room temperature is cooled to after boiling 0.5h at 150 DEG C, then centrifugation (10000rpm, 30min, 4 DEG C) obtain supernatant liquor and throw out.Supernatant concentration is obtained pea soluble polysaccharide concentrated solution, then namely warm air drying obtains water-soluble pea polysaccharide, and yield is 10.87%.The k value that the aqueous solution being configured to 10% records at 20 DEG C is 99mPas.
Embodiment 4
The abundant stirring and dissolving 1h of appropriate amount of deionized water is added in waste pea slag after extracting starch and from albumen to pea, with (7500rpm centrifugal after sodium hydroxide regulation system pH8.0,25min, 4 DEG C), abandoning supernatant is to remove deproteinize, residue precipitation adds the deionized water of 25 times of quality, with hydrochloric acid regulation system pH5.5 under fully stirring, and room temperature is cooled to after boiling 1h at 110 DEG C, then centrifugation (10000rpm, 30min, 4 DEG C) obtain supernatant liquor and throw out.Supernatant concentration is obtained pea soluble polysaccharide concentrated solution, then namely lyophilize obtains water-soluble pea polysaccharide, and yield is 15.17%.The k value that the aqueous solution being configured to 10% records at 20 DEG C is 105mPas.
Embodiment 5
The abundant stirring and dissolving 1h of appropriate amount of deionized water is added in waste pea slag after extracting starch and from albumen to pea, with (7500rpm centrifugal after sodium hydroxide regulation system pH8.0,25min, 4 DEG C), abandoning supernatant is to remove deproteinize, residue precipitation adds the deionized water of 30 times of quality, with hydrochloric acid regulation system pH6.0 under fully stirring, and room temperature is cooled to after boiling 3h at 105 DEG C, then centrifugation (10000rpm, 30min, 4 DEG C) obtain supernatant liquor and throw out.Supernatant concentration is obtained pea soluble polysaccharide concentrated solution, then namely spraying dry obtains water-soluble pea polysaccharide, and yield is 13.29%.The k value that the aqueous solution being configured to 10% records at 20 DEG C is 100mPas.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (8)
1. a preparation method for water-soluble pea polysaccharide, is characterized in that comprising following preparation process:
Get the dry bean dregs of pea, add deionized water, dissolving 0.5 ~ 1h, is centrifugal after 8.0 ~ 10.0 with alkali regulation system pH, removing supernatant liquor, the deionized water of 10 ~ 30 times of quality is added in precipitation, be 4.0 ~ 7.0 with sour regulation system pH, at 105 ~ 125 DEG C, boiling was cooled to room temperature after 0.5 ~ 3 hour, then obtained pea soluble polysaccharide concentrated solution through centrifugal, concentrated, drying, obtains water-soluble pea polysaccharide.
2. the preparation method of a kind of water-soluble pea polysaccharide according to claim 1, is characterized in that: the dry bean dregs of described pea refer to the waste pea slag after pea extraction starch and albumen.
3. the preparation method of a kind of water-soluble pea polysaccharide according to claim 1, is characterized in that: described precipitation is 1:(20 ~ 30 with the mass ratio of the deionized water added).
4. the preparation method of a kind of water-soluble pea polysaccharide according to claim 1, it is characterized in that: described alkali refers to sodium hydroxide, described acid refers to hydrochloric acid.
5. the preparation method of a kind of water-soluble pea polysaccharide according to claim 1, is characterized in that: the sour regulation system pH of described use is 5.0 ~ 6.0.
6. the preparation method of a kind of water-soluble pea polysaccharide according to claim 1, it is characterized in that: described boiling temperature is 110 ~ 120 DEG C, cooking time is 1.5 ~ 2h.
7. the preparation method of a kind of water-soluble pea polysaccharide according to claim 1, is characterized in that: described drying refers to spraying dry, warm air drying or vacuum lyophilization.
8. a water-soluble pea polysaccharide, is characterized in that: prepared by the method described in any one of claim 1 ~ 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610099969.8A CN105542028A (en) | 2016-02-23 | 2016-02-23 | Water-soluble pea polysaccharide and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610099969.8A CN105542028A (en) | 2016-02-23 | 2016-02-23 | Water-soluble pea polysaccharide and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105542028A true CN105542028A (en) | 2016-05-04 |
Family
ID=55821617
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610099969.8A Pending CN105542028A (en) | 2016-02-23 | 2016-02-23 | Water-soluble pea polysaccharide and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105542028A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1076700A (en) * | 1992-03-23 | 1993-09-29 | 不二制油株式会社 | water-soluble polysaccharide and preparation method thereof |
| JP2003082003A (en) * | 2001-07-05 | 2003-03-19 | Mitsubishi Rayon Co Ltd | Method for purifying water-soluble seed polysaccharides |
| CN103608360A (en) * | 2011-06-24 | 2014-02-26 | 不二制油株式会社 | Pectic polysaccharide and method for producing same |
| CN104017099A (en) * | 2014-06-12 | 2014-09-03 | 苏州经贸职业技术学院 | Microwave extraction, separation and purification method of pea polysaccharide |
-
2016
- 2016-02-23 CN CN201610099969.8A patent/CN105542028A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1076700A (en) * | 1992-03-23 | 1993-09-29 | 不二制油株式会社 | water-soluble polysaccharide and preparation method thereof |
| JP2003082003A (en) * | 2001-07-05 | 2003-03-19 | Mitsubishi Rayon Co Ltd | Method for purifying water-soluble seed polysaccharides |
| CN103608360A (en) * | 2011-06-24 | 2014-02-26 | 不二制油株式会社 | Pectic polysaccharide and method for producing same |
| CN104017099A (en) * | 2014-06-12 | 2014-09-03 | 苏州经贸职业技术学院 | Microwave extraction, separation and purification method of pea polysaccharide |
Non-Patent Citations (1)
| Title |
|---|
| 汪洪涛等: ""豆渣中水溶性大豆多糖提取工艺的优化"", 《江苏农业科学》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105481993A (en) | Tremella polysaccharide extracting method | |
| CN104211827B (en) | A kind of method extracting polysaccharide from Hericium erinaceus (Bull. Ex Fr.) Pers. dreg | |
| CN106011199B (en) | Pretreatment method of crop straws | |
| CN102796163A (en) | Method for extracting and separating proteins from cake by using ionic liquid and enzyme process | |
| CN101120766B (en) | Monosodium glutamate green manufacturing technology | |
| CN103330047B (en) | Preparation method of rice protein | |
| CN105754015A (en) | Method for extracting chitin from oyster shell | |
| CN110194807B (en) | Method for extracting water-soluble active substance of coix seeds | |
| CN102334586A (en) | Method for preparing tea leaf protein emulsifier from tea leaves | |
| CN102599326B (en) | Backward extraction method for reversed micellar extraction of soybean protein | |
| CN103665195A (en) | Method for extracting agar from gracilaria with enzymatic method | |
| CN108840916A (en) | A kind of double enzyme optimization assisted extraction vegetable seed globulin and albuminised process | |
| CN104561129A (en) | Enzymolysis saccharification processing method of straw | |
| CN107242347B (en) | Method for improving soybean protein foamability by using enzyme composite treatment by taking soybean meal as raw material | |
| CN106752072A (en) | A kind of High color values capsanthin continuous production processes | |
| CN101260129A (en) | Method of purifying tannic acid | |
| CN102643364A (en) | Method for extracting ganoderan from submerged-fermentation mycelia of ganoderma lucidum | |
| CN105542028A (en) | Water-soluble pea polysaccharide and preparation method thereof | |
| CN105192663A (en) | Preparation method for black tea essence | |
| CN1277486C (en) | Modification method of soybean protein concentrate | |
| CN103265649B (en) | The method of pectin is extracted from lemon peel | |
| CN100532396C (en) | Process for preparing high purity pectin by using apple pomace | |
| CN105385738B (en) | A kind of preparation method of yak cheese protein phosphatase polypeptide | |
| CN101766254A (en) | Method for preparing low-sodium healthcare isolated soy protein | |
| CN106749686B (en) | A kind of preparation method of microcrystalline cellulose |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160504 |
|
| RJ01 | Rejection of invention patent application after publication |