CN105541937A - Extracting method for stevioside in stevia rebaudiana - Google Patents
Extracting method for stevioside in stevia rebaudiana Download PDFInfo
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- CN105541937A CN105541937A CN201610034444.6A CN201610034444A CN105541937A CN 105541937 A CN105541937 A CN 105541937A CN 201610034444 A CN201610034444 A CN 201610034444A CN 105541937 A CN105541937 A CN 105541937A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/256—Polyterpene radicals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
Abstract
The invention relates to an extracting method for stevioside in stevia rebaudiana. The extracting method comprises the following steps: extracting dry leaves of the stevia rebaudiana and remaining an extracting liquid; loading a sample of the extracting liquid to a separation column which is filled with Generik RP packing for separating a target component from impurity components in the extracting liquid; remaining the target component in the Generik RP packing into which the sample is loaded; subsequently, performing gradient elution on the target component by respectively adopting a pretreatment eluting liquid and a target eluting liquid, respectively obtaining a pretreated effluent liquid and a target effluent liquid, and remaining the target effluent liquid, wherein the pretreatment eluting liquid is a mixed liquid of an organic solvent and water, and the target eluting liquid is a mixed liquid of the organic solvent and water or the organic solvent; concentrating and drying the target effluent liquid to obtain the stevioside. The extracting method for the stevioside in the stevia rebaudiana has few steps and simple overall process, and is beneficial to application.
Description
Technical field
The present invention relates to technical field of natural product extraction, particularly relate to the extracting method of steviol glycoside in a kind of sweet Stevia.
Background technology
Sweet Stevia another name stevia rebaudianum, sugar grass, belong to composite family, containing 14 kinds of trace elements, 32 kinds of nutritive ingredients, not metabolism in vivo, do not accumulate, not carcinogenic, nontoxicity, its security has obtained the accreditation of the tissue such as international FAO and WHO and U.S. FAD, therefore it is fabulous sugared source, is again good source of nutrition.
Steviol glycoside is the effective constituent in sweet Stevia, belongs to the intensive sweetener of natural diet.It contains the tetracyclic diterpene monomer of multiple heterogeneity, these monomers have identical aglycon-steviol (Steviol), can according to C13 and C19 position link different aglycons be divided into stevioside (Stevioside), rebaudioside A (RebaudiosideA), B (RebaudiosideB), C (RebaudiosideC), D (RebaudiosideD), E (RebaudiosideE), Dole can glycosides (DulcosideA), this is for dimension uncle's glycosides (Steviolbioside) and immature fruit of Juteleaf Raspberry glycosides (Rubusoside).In these monomers, that content is the highest is St (Stevioside), RA (RebaudiosideA) and Rc (RebaudiosideC), account for 5% ~ 9.5% of leaf dry weight, 2% ~ 4.5% and 1% ~ 2% respectively, sugariness is respectively 300,450 and 120 times of sucrose.Because steviol glycoside has above-mentioned high sugariness, characteristic low in calories, long-term eating can not make people get fat, and therefore, current steviol glycoside substitutes sucrose as a kind of sweeting agent, it continues outside sucrose beet sugar, and the third has Development volue and healthy natural substitute of praising highly.I crosses the Ministry of Health and ratifies natural sweeteners that steviol glycoside is quantity-unlimiting use and pharmaceutical sweeting agent auxiliary material respectively from 1985 and nineteen ninety.Therefore, steviol glycoside has a wide range of applications scope and broad market outlook.
But, in traditional sweet Stevia the extracting method of steviol glycoside be by sweet Stevia cured leaf by lixiviate, filtration, ultrafiltration, then add ferrous sulfate, milk of lime obtain supernatant liquor, iterate through resin, finally dry steviol glycoside.Step is more, and integrated artistic is loaded down with trivial details, is unfavorable for application.
Summary of the invention
Based on this, be necessary the problem that in the extracting method for steviol glycoside in traditional sweet Stevia, integrated artistic is loaded down with trivial details, the extracting method of steviol glycoside in the simple sweet Stevia of a kind of technique is provided.
In sweet Stevia, an extracting method for steviol glycoside, comprises the steps:
Vat liquor is retained by after the lixiviate of sweet Stevia cured leaf;
By described vat liquor loading to the separator column being filled with GenerikRP filler, in order to be separated target components in described vat liquor and impurity composition, described target components is remained with in described GenerikRP filler after loading, pre-treatment elutriant and target elutriant is adopted to carry out gradient elution to described target components subsequently respectively, obtain pretreated stream fluid and object flow fluid respectively, and retain object flow fluid, described pre-treatment elutriant is the mixed solution of organic solvent and water, and described target elutriant is mixed solution or the organic solvent of organic solvent and water; And
Concentrate drying is carried out to described object flow fluid, obtains steviol glycoside.
Compared with the extracting method of steviol glycoside in traditional sweet Stevia, in above-mentioned sweet Stevia steviol glycoside extracting method in, only by vat liquor loading to separator column, then need carry out wash-out and retain object flow fluid, carrying out concentrate drying afterwards can obtain steviol glycoside.Without the need to adding ferrous sulfate, milk of lime etc., also do not need to iterate through resin, therefore, step is less, and integrated artistic is comparatively simple, is conducive to application.
Wherein in an embodiment, described separator column is solid-phase extraction column, flash chromatography post or liquid-phase chromatographic column.
Wherein in an embodiment, described organic solvent is methyl alcohol, ethanol, Virahol, acetone or acetonitrile.
Wherein in an embodiment, in described pre-treatment elutriant, the volume ratio of described organic solvent and water is 0:1 ~ 3:7;
In described target elutriant, the volume ratio of described organic solvent and water is 3:7 ~ 1:0.
Wherein in an embodiment, the organic solvent in described pre-treatment elutriant and the volume ratio of water are 1:4;
Described target elutriant comprises first object elutriant and the second target elutriant, and the organic solvent in described first object elutriant and the volume ratio of water are 2:3, and the organic solvent in described second target elutriant and the volume ratio of water are 1:1.
Wherein in an embodiment, by described vat liquor loading to being filled with in the operation of separator column of GenerikRP filler, the mass ratio of the solute in described vat liquor and described GenerikRP filler is 1:100 ~ 1:10, and described solute is made up of RA and St.
Accompanying drawing explanation
Fig. 1 is the schema of the extracting method of steviol glycoside in the sweet Stevia of an embodiment;
Fig. 2 is the high-efficient liquid phase chromatogram of vat liquor in embodiment 1;
Fig. 3 is the high-efficient liquid phase chromatogram of the effluent liquid collected in embodiment 1.
Embodiment
For enabling above-mentioned purpose of the present invention, feature and advantage become apparent more, are described in detail the specific embodiment of the present invention below in conjunction with accompanying drawing.Set forth a lot of detail in the following description so that fully understand the present invention.But the present invention can be much different from alternate manner described here to implement, those skilled in the art can when without prejudice to doing similar improvement when intension of the present invention, therefore the present invention is by the restriction of following public specific embodiment.
Unless otherwise defined, all technology used herein and scientific terminology are identical with belonging to the implication that those skilled in the art of the present invention understand usually.The object of term used in the description of the invention herein just in order to describe specific embodiment, is not intended to be restriction the present invention.Term as used herein " and/or " comprise arbitrary and all combinations of one or more relevant Listed Items.
In the sweet Stevia of an embodiment as shown in Figure 1, the extracting method of steviol glycoside, comprises the steps:
S10: retain vat liquor by after the lixiviate of sweet Stevia cured leaf.
Being operating as of vat liquor is retained: mixed with water by sweet Stevia cured leaf and carry out lixiviate by after the lixiviate of sweet Stevia cured leaf, maintaining extraction temperature is 80 DEG C ~ 90 DEG C, extraction time is 20min ~ 60min, carry out solid-liquid separation afterwards, obtain solid and liquid, solid is mixed with water, and repeat at least twice above-mentioned leaching step, retain the liquid after each lixiviate, obtain vat liquor.
Wherein, the mass ratio of sweet Stevia cured leaf and water is 1:10 ~ 1:12.The mass ratio of solid and water is 1:10 ~ 1:12.Select above-mentioned mass ratio can by the useful component lixiviate in sweet Stevia cured leaf out.
Vat liquor is the extracting solution of steviol glycoside crude product.
S20: by the vat liquor loading of step S10 to the separator column being filled with GenerikRP filler, in order to be separated target components in vat liquor and impurity composition, target components is remained with in GenerikRP filler after loading, pre-treatment elutriant and target elutriant is adopted to carry out gradient elution to target components subsequently respectively, obtain pretreated stream fluid and object flow fluid respectively, and retain object flow fluid, pre-treatment elutriant and target elutriant are the mixed solution of organic solvent and water, and the ratio of organic solvent and water is less than the ratio of organic solvent and water in target elutriant in pre-treatment elutriant.
Separator column is solid-phase extraction column, flash chromatography post or liquid-phase chromatographic column.Wherein, solid-phase extraction column (English SolidPhaseExtractionColumn, be called for short SPEcolumn, or SolidPhaseextractionCartridges, be called for short SPEcartridges) be from chromatography column develop a kind of for extracting, being separated, concentrated sample pretreatment device.Flash chromatography post is also called Flash post, refers generally to the SiO got express developed that can pressurize
2pillar.The development trend of liquid-phase chromatographic column is that reduction filler granularity and post footpath are imitated to improve post.
GenerikRP filler is modification bonded silica gel filler, and its bonded functional group is C4 ~ C18 alkyl.The particle diameter of GenerikRP filler is 10 μm ~ 200 μm.
Organic solvent is methyl alcohol, ethanol, Virahol, acetone or acetonitrile.
In pre-treatment elutriant, the volume ratio of organic solvent and water is 0:1 ~ 3:7.
In target elutriant, the volume ratio of organic solvent and water is 3:7 ~ 1:0.When the volume ratio of organic solvent and water is 1:0, target elutriant is pure organic solvent.
In a preferred embodiment, the organic solvent in pre-treatment elutriant and the volume ratio of water are 1:4.Target elutriant comprises first object elutriant and the second target elutriant, and the organic solvent in first object elutriant and the volume ratio of water are 2:3, and the organic solvent in the second target elutriant and the volume ratio of water are 1:1.
By vat liquor loading to being filled with in the operation of separator column of GenerikRP filler, the mass ratio of the solute in vat liquor and GenerikRP filler is 1:100 ~ 1:10, and solute is made up of RA and St.
S30: concentrate drying is carried out to the object flow fluid in step S20, obtains steviol glycoside.
Due to content in steviol glycoside the highest be St (Stevioside), RA (RebaudiosideA) and Rc (RebaudiosideC), the content of steviol glycoside that therefore the application obtains is as the criterion with the content of steviol glycoside St, RA and Rc.
Compared with the extracting method of steviol glycoside in traditional sweet Stevia, in above-mentioned sweet Stevia steviol glycoside extracting method in, only by vat liquor loading to separator column, then need carry out wash-out and retain object flow fluid, carrying out concentrate drying afterwards can obtain steviol glycoside.Without the need to adding ferrous sulfate, milk of lime etc., also do not need to iterate through resin, therefore, step is less, and integrated artistic is comparatively simple, is conducive to application.
Be embodiment below:
Embodiment 1
Mixed with the pure water of 200g by 25g sweet Stevia cured leaf and carry out lixiviate, maintaining extraction temperature is 90 DEG C, and extraction time is 40min, carries out solid-liquid separation afterwards, obtains solid and liquid.Continued to mix with the pure water of 200g by above-mentioned solid, above-mentioned leaching step in triplicate, retains the liquid after each lixiviate, obtains vat liquor, finally adds ethanol and makes proportion of ethanol in vat liquor reach 10%.Carry out HPLC (high performance liquid chromatography) test to vat liquor, as shown in Figure 2, result shows, except containing except steviol glycoside St, RA and Rc in vat liquor, the peak value of other impurity is very high, shows that the content of impurity in vat liquor is very high.
By above-mentioned vat liquor loading extremely in advance with the solid-phase extraction column being filled with 30gGenerikRP filler that 10% methanol aqueous solution is equilibrated, pre-treatment elutriant, first object elutriant and the second target elutriant is adopted to carry out gradient elution to target components subsequently respectively, be all 15 column volumes, obtain pretreated stream fluid, first object effluent liquid and the second object flow fluid respectively, and retain first object effluent liquid and the second object flow fluid.Wherein, pre-treatment elutriant is the mixed solution of methyl alcohol (MeOH) and water, and the volume ratio of methyl alcohol and water is 1:4.The mixed solution of first object eluant methanol and water, the volume ratio of methyl alcohol and water is 2:3.Second target elutriant is the mixed solution of methyl alcohol and water, and the volume ratio of methyl alcohol and water is 1:1.
Collect first object effluent liquid and the second object flow fluid, and carry out HPLC test to it, as shown in Figure 3, result shows, the total content of steviol glycoside St, RA and Rc that embodiment 1 is extracted is 90.4%.Subsequently concentrate drying is carried out to it, obtain steviol glycoside.
Embodiment 2
Mixed with the pure water of 200g by 25g sweet Stevia cured leaf and carry out lixiviate, maintaining extraction temperature is 90 DEG C, and extraction time is 40min, carries out solid-liquid separation afterwards, obtains solid and liquid.Continued to mix with the pure water of 200g by above-mentioned solid, above-mentioned leaching step in triplicate, retains the liquid after each lixiviate, obtains vat liquor, finally adds ethanol and makes proportion of ethanol in vat liquor reach 10%.Carry out HPLC (high performance liquid chromatography) test to vat liquor, as shown in Figure 2, result shows, except containing except steviol glycoside St, RA and Rc in vat liquor, the peak value of other impurity is very high, shows that the content of impurity in vat liquor is very high.
By above-mentioned vat liquor loading extremely in advance with the FLASH post being filled with 30gGenerikRP filler that 10% methanol aqueous solution is equilibrated, pre-treatment elutriant, first object elutriant and the second target elutriant is adopted to carry out gradient elution to target components subsequently respectively, be all 15 column volumes, obtain pretreated stream fluid, first object effluent liquid and the second object flow fluid respectively, and retain first object effluent liquid and the second object flow fluid.Wherein, pre-treatment elutriant is the mixed solution of methyl alcohol (MeOH) and water, and the volume ratio of methyl alcohol and water is 1:4.The mixed solution of first object eluant methanol and water, the volume ratio of methyl alcohol and water is 2:3.Second target elutriant is the mixed solution of methyl alcohol and water, and the volume ratio of methyl alcohol and water is 1:1.
Collect first object effluent liquid and the second object flow fluid, and carry out HPLC test to it, result shows, the total content of steviol glycoside St, RA and Rc that embodiment 2 is extracted is 90.2%.Subsequently concentrate drying is carried out to it, obtain steviol glycoside.
Embodiment 3
Mixed with the pure water of 200g by 25g sweet Stevia cured leaf and carry out lixiviate, maintaining extraction temperature is 90 DEG C, and extraction time is 40min, carries out solid-liquid separation afterwards, obtains solid and liquid.Continued to mix with the pure water of 200g by above-mentioned solid, above-mentioned leaching step in triplicate, retains the liquid after each lixiviate, obtains vat liquor, finally adds ethanol and makes proportion of ethanol in vat liquor reach 10%.Carry out HPLC (high performance liquid chromatography) test to vat liquor, as shown in Figure 2, result shows, except containing except steviol glycoside St, RA and Rc in vat liquor, the peak value of other impurity is very high, shows that the content of impurity in vat liquor is very high.
By above-mentioned vat liquor loading extremely in advance with the liquid-phase chromatographic column being filled with 30gGenerikRP filler that 10% methanol aqueous solution is equilibrated, pre-treatment elutriant, first object elutriant and the second target elutriant is adopted to carry out gradient elution to target components subsequently respectively, be all 15 column volumes, obtain pretreated stream fluid, first object effluent liquid and the second object flow fluid respectively, and retain first object effluent liquid and the second object flow fluid.Wherein, pre-treatment elutriant is the mixed solution of methyl alcohol (MeOH) and water, and the volume ratio of methyl alcohol and water is 1:4.The mixed solution of first object eluant methanol and water, the volume ratio of methyl alcohol and water is 2:3.Second target elutriant is the mixed solution of methyl alcohol and water, and the volume ratio of methyl alcohol and water is 1:1.
Collect first object effluent liquid and the second object flow fluid, and carry out HPLC test to it, result shows, the total content of steviol glycoside St, RA and Rc that embodiment 3 is extracted is 89.2%.Subsequently concentrate drying is carried out to it, obtain steviol glycoside.
Each technical characteristic of the above embodiment can combine arbitrarily, for making description succinct, the all possible combination of each technical characteristic in above-described embodiment is not all described, but, as long as the combination of these technical characteristics does not exist contradiction, be all considered to be the scope that this specification sheets is recorded.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be construed as limiting the scope of the patent.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (6)
1. the extracting method of steviol glycoside in sweet Stevia, is characterized in that, comprise the steps:
Vat liquor is retained by after the lixiviate of sweet Stevia cured leaf;
By described vat liquor loading to the separator column being filled with GenerikRP filler, in order to be separated target components in described vat liquor and impurity composition, described target components is remained with in described GenerikRP filler after loading, pre-treatment elutriant and target elutriant is adopted to carry out gradient elution to described target components subsequently respectively, obtain pretreated stream fluid and object flow fluid respectively, and retain object flow fluid, described pre-treatment elutriant is the mixed solution of organic solvent and water, and described target elutriant is mixed solution or the organic solvent of organic solvent and water; And
Concentrate drying is carried out to described object flow fluid, obtains steviol glycoside.
2. the extracting method of steviol glycoside in sweet Stevia according to claim 1, it is characterized in that, described separator column is solid-phase extraction column, flash chromatography post or liquid-phase chromatographic column.
3. the extracting method of steviol glycoside in sweet Stevia according to claim 1, it is characterized in that, described organic solvent is methyl alcohol, ethanol, Virahol, acetone or acetonitrile.
4. the extracting method of steviol glycoside in sweet Stevia according to claim 1, it is characterized in that, in described pre-treatment elutriant, the volume ratio of described organic solvent and water is 0:1 ~ 3:7;
In described target elutriant, the volume ratio of described organic solvent and water is 3:7 ~ 1:0.
5. the extracting method of steviol glycoside in sweet Stevia according to claim 1, it is characterized in that, the organic solvent in described pre-treatment elutriant and the volume ratio of water are 1:4;
Described target elutriant comprises first object elutriant and the second target elutriant, and the organic solvent in described first object elutriant and the volume ratio of water are 2:3, and the organic solvent in described second target elutriant and the volume ratio of water are 1:1.
6. the extracting method of steviol glycoside in sweet Stevia according to claim 1, it is characterized in that, by described vat liquor loading to being filled with in the operation of separator column of GenerikRP filler, the mass ratio of the solute in described vat liquor and described GenerikRP filler is 1:100 ~ 1:10, and described solute is made up of RA and St.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107937380A (en) * | 2017-11-29 | 2018-04-20 | 蚌埠市华东生物科技有限公司 | A kind of STEVIA REBAUDIANA extracts the carrier immobilized Trichoderma viride of steviol glycoside |
| CN107988437A (en) * | 2017-11-29 | 2018-05-04 | 甘肃西域阳光食品有限公司 | A kind of technique that STEVIA REBAUDIANA immersion ability is strengthened by vibrating screen |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006038221A1 (en) * | 2004-10-04 | 2006-04-13 | Council Of Scientific And Industrial Research | Process for production of steviosides from stevia rebaudiana bertoni |
| CN101220062A (en) * | 2008-01-23 | 2008-07-16 | 石任兵 | Method for preparing stevioside and rebaudiodside A simultaneously |
| CN101270138A (en) * | 2007-03-23 | 2008-09-24 | 宁波绿之健药业有限公司 | Extracting method for high-content sweet inulin A3 |
| CN101946887A (en) * | 2010-09-01 | 2011-01-19 | 桂林莱茵生物科技股份有限公司 | Preparation method of stevioside |
| WO2013157887A1 (en) * | 2012-04-19 | 2013-10-24 | 아주대학교산학협력단 | Recombinant microorganism having kaurene, kaurenoic acid or steviol producing ability, and method for preparing kaurene, kaurenoic acid or steviol using same |
-
2016
- 2016-01-19 CN CN201610034444.6A patent/CN105541937B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006038221A1 (en) * | 2004-10-04 | 2006-04-13 | Council Of Scientific And Industrial Research | Process for production of steviosides from stevia rebaudiana bertoni |
| CN101270138A (en) * | 2007-03-23 | 2008-09-24 | 宁波绿之健药业有限公司 | Extracting method for high-content sweet inulin A3 |
| CN101220062A (en) * | 2008-01-23 | 2008-07-16 | 石任兵 | Method for preparing stevioside and rebaudiodside A simultaneously |
| CN101946887A (en) * | 2010-09-01 | 2011-01-19 | 桂林莱茵生物科技股份有限公司 | Preparation method of stevioside |
| WO2013157887A1 (en) * | 2012-04-19 | 2013-10-24 | 아주대학교산학협력단 | Recombinant microorganism having kaurene, kaurenoic acid or steviol producing ability, and method for preparing kaurene, kaurenoic acid or steviol using same |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107937380A (en) * | 2017-11-29 | 2018-04-20 | 蚌埠市华东生物科技有限公司 | A kind of STEVIA REBAUDIANA extracts the carrier immobilized Trichoderma viride of steviol glycoside |
| CN107988437A (en) * | 2017-11-29 | 2018-05-04 | 甘肃西域阳光食品有限公司 | A kind of technique that STEVIA REBAUDIANA immersion ability is strengthened by vibrating screen |
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