CN105536075A - Nano-micron fiber, chitosan and polylactic acid composite stent and making method thereof - Google Patents
Nano-micron fiber, chitosan and polylactic acid composite stent and making method thereof Download PDFInfo
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- CN105536075A CN105536075A CN201610106720.5A CN201610106720A CN105536075A CN 105536075 A CN105536075 A CN 105536075A CN 201610106720 A CN201610106720 A CN 201610106720A CN 105536075 A CN105536075 A CN 105536075A
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- 239000000835 fiber Substances 0.000 title claims abstract description 62
- 229920001661 Chitosan Polymers 0.000 title claims abstract description 59
- 229920000747 poly(lactic acid) Polymers 0.000 title claims abstract description 59
- 239000004626 polylactic acid Substances 0.000 title claims abstract description 59
- 239000002131 composite material Substances 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title abstract description 13
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims description 17
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 16
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 16
- APSBXTVYXVQYAB-UHFFFAOYSA-M sodium docusate Chemical compound [Na+].CCCCC(CC)COC(=O)CC(S([O-])(=O)=O)C(=O)OCC(CC)CCCC APSBXTVYXVQYAB-UHFFFAOYSA-M 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 150000001875 compounds Chemical class 0.000 claims description 12
- 239000002904 solvent Substances 0.000 claims description 12
- 238000009987 spinning Methods 0.000 claims description 9
- 229920002101 Chitin Polymers 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 239000002121 nanofiber Substances 0.000 claims description 2
- 239000012528 membrane Substances 0.000 abstract description 7
- 230000007547 defect Effects 0.000 abstract description 2
- 238000007710 freezing Methods 0.000 abstract 2
- 230000008014 freezing Effects 0.000 abstract 2
- 238000007605 air drying Methods 0.000 abstract 1
- 239000003513 alkali Substances 0.000 abstract 1
- 230000004071 biological effect Effects 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 239000010408 film Substances 0.000 description 9
- 238000003756 stirring Methods 0.000 description 8
- 239000010409 thin film Substances 0.000 description 5
- 239000005030 aluminium foil Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 2
- 239000002114 nanocomposite Substances 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 description 1
- 229920001287 Chondroitin sulfate Polymers 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229940059329 chondroitin sulfate Drugs 0.000 description 1
- 210000000589 cicatrix Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002062 molecular scaffold Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/042—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/06—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/16—Biologically active materials, e.g. therapeutic substances
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- A—HUMAN NECESSITIES
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
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- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Heart & Thoracic Surgery (AREA)
- Transplantation (AREA)
- Dermatology (AREA)
- Vascular Medicine (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Surgery (AREA)
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- Molecular Biology (AREA)
- Biomedical Technology (AREA)
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- Materials For Medical Uses (AREA)
- Nonwoven Fabrics (AREA)
- Artificial Filaments (AREA)
Abstract
The invention relates to a nano-micron fiber, chitosan and polylactic acid composite stent and a making method thereof. According to the making method, a nanometer chitosan and polylactic acid composite fiber membrane and a micrometer chitosan and polylactic acid composite fiber membrane are subjected to electrostatic spinning under certain conditions; after the composite fiber membranes are subjected to alkali washing and air drying, the nanometer fiber membrane and the micrometer fiber membrane are cut into short fibers in water at a high speed according to a certain proportion; after sample separation, freezing forming is performed at the temperature of -18 DEG C, and then freezing drying is performed to obtain the nano-micron fiber, chitosan and polylactic acid composite three-dimensional stent. Defects of chitosan and polylactic acid are overcome after chitosan and polylactic acid are compounded. Through the making method, the process is simple, the stent has a micro-nano scale in fiber diameter, and the composite stent has good biological properties and mechanical properties.
Description
Technical field
The present invention relates to a kind of nano micron fibre chitosan polylactic acid compound rest and preparation method thereof, belong to biology medical material technical field.
Background technology
Chitosan is as a kind of cation alkaline polysaccharide, the polysaccharose substance such as chondroitin sulfate and hyaluronic acid of similar in human body, can be degraded and absorbed in vivo, and have pain relieving, hemostasis, antibacterial, promote wound healing, reduce the excellent specific property such as cicatrix have important application in biomedical materials field at present.The people such as SundararajanV adopt phase detachment technique to prepare chitosan stent first, and its structure is tens of lamellar honeycomb textures to hundreds of micron thickness, has good biology performance (Biomaterials, 1999; 20:1133-1142), but chitosan is be easy to swelling as the major defect of timbering material.A kind of synthetic polymer timbering material that polylactic acid is ratified the earliest as U.S. FDA; have nontoxic, good with human compatibility, the advantage such as degradablely can to absorb in vivo; be widely used in the reparation (LeeEJ etc. of the tissues such as bone, cartilage, blood vessel and skin and organ; AnnBiomedEng, 2014; 42 (2): 323), polylactic acid hydrophobicity is strong, and cellular affinity is poor, and therefore chitosan and polylactic acid compound can overcome both shortcomings.It is composite porous and preparation method thereof that Li Geng discloses a kind of chitosan-polylactic acid, shows that the composite of chitosan and polylactic acid has good performance (CN102757625A).
Research shows that nano fiber scaffold has larger specific surface area, can provide the better condition of cell adhesion and differentiation, but simple nanofiber mechanical strength is lower, cannot bear the mechanical loading of stent implantation procedure.Comparatively speaking, micrometer fibers support has good mechanical property.Therefore, three-dimensional rack fibre diameter with micro nanometer fiber has good biocompatibility and mechanical property.The method that the many employings of current researcher are separated prepares three-dimensional rack, but its fibre diameter uncontrollable, or adopt the method for electrostatic spinning to obtain the fiber membrane of two dimension, but the 3 D chitosan polylactic acid compound rest preparation method how preparing micro nanometer fiber there is not yet report.
Summary of the invention
Main imagination of the present invention adopts the method for electrostatic spinning to prepare nanometer and micron chitosan PLA Composite Fiber thin film respectively, according to a certain ratio by nanometer and the mixing of micron composite cellulosic membrane, and laminated film high speed shear is become short fiber, after dividing sample, freeze forming, obtains nano micron fibre chitosan polylactic acid complex three-dimensional support through lyophilization.
In the present invention the preparation method of nano micron fibre chitosan polylactic acid complex three-dimensional support specific as follows described in.
(1) electrostatic spinning nano chitin fiber film: preparation chitosan 5.0% solution, solvent be trifluoroacetic acid with the mixture of diox, volume ratio is 70:30; Preparation polylactic acid 10.0% solution, solvent be chloroform with the mixture of diox, volume ratio is 70:30.Chitosan and PLA solution are added respectively in two syringes, connected by two syringes with two-in-one syringe needle, the voltage passing to 20 ~ 25kV carries out spinning.
(2) electrostatic spinning micron chitin fiber film: preparation chitosan 7.5% solution, solvent be trifluoroacetic acid with the mixture of diox, volume ratio is 90:10; Preparation polylactic acid 20.0% solution, solvent be chloroform with the mixture of diox, volume ratio is 90:10.Chitosan and PLA solution are added respectively in two syringes, connected by two syringes with two-in-one syringe needle, the voltage passing to 12 ~ 15kV carries out spinning.
(3) nanometer obtained by electrostatic spinning and micron chitosan PLA Composite Fiber film are immersed in the solvent removed in the sodium hydroxide solution of 0.5mol/L and do not volatilize, and wash air-dry rear stand-by.
(4) take quantitative nanometer and micron chitosan PLA Composite Fiber film respectively, join in quantitative water, cut into short fiber in water high speed.
(5) divide sample in weighing botle, freeze forming at-18 DEG C.
(6) nano micron fibre chitosan polylactic acid compound rest can be obtained after lyophilization.
The nano micron fibre chitosan polylactic acid compound rest outward appearance that the present invention obtains is white disk, and its shape and height can adopt different containers and point sample volume-adjustment in point sample.The compound of chitosan and polylactic acid can overcome both shortcomings, and support has better combination property.Obtain mainly through electrostatic spinning in preparation method and receive the fiber of micro-meter scale, also can adjust spinning condition in preparation easily and obtain nanometer and micron chitosan PLA Composite Fiber.This support has micro-nano-scale on fibre diameter, gives the good biocompatibility of support and mechanical strength.This preparation method technique is simple, and be easy to the chitosan PLA Composite Fiber obtaining different-diameter, compared to simple method of electrostatic spinning, this method also easily can prepare the support of three dimensional structure.
Detailed description of the invention
Below in conjunction with specific embodiment, content of the present invention is further described, but implementation of the present invention is not limited thereto.
Embodiment 1: trifluoroacetic acid and the 3ml diox of getting 7ml respectively, mix homogeneously, takes 0.5g chitosan, stirring and dissolving, leaves standstill 12 hours.Get chloroform and the 3ml diox of 7ml respectively, mix homogeneously, takes 1.0g polylactic acid, stirring and dissolving, leaves standstill 12 hours.Chitosan and PLA solution are joined respectively in two syringes, with two-in-one syringe needle, two syringes are connected, pass to the voltage spinning of 20kV, be collected on the aluminium foil of ground connection, can obtain chitosan polylactic acid nano composite fiber thin film, fibre diameter is between 50-500nm.Get trifluoroacetic acid and the 1ml diox of 9ml respectively, mix homogeneously, takes 0.75g chitosan, stirring and dissolving, leaves standstill 12 hours.Get chloroform and the 1ml diox of 9ml respectively, mix homogeneously, takes 2.0g polylactic acid, stirring and dissolving, leaves standstill 12 hours.Chitosan and PLA solution are joined respectively in two syringes, with two-in-one syringe needle, two syringes are connected, pass to the voltage spinning of 15kV, be collected on the aluminium foil of ground connection, can obtain chitosan polylactic acid micron composite fiber thin film, fibre diameter is between 1-10 μm.The nanometer obtained by electrostatic spinning and micrometer fibers film are immersed in the solvent removed in the sodium hydroxide solution of 0.5mol/L and do not volatilize, and wash air-dry.Take 0.2g and 0.8g nanometer and micron chitosan PLA Composite Fiber film respectively, short fiber is cut in 100ml water high speed, divide sample in weighing botle, freeze forming at-18 DEG C, can obtain nano micron fibre chitosan polylactic acid complex three-dimensional support in freeze dryer after lyophilizing.
Embodiment 2: trifluoroacetic acid and the 6ml diox of getting 14ml respectively, mix homogeneously, takes 1.0g chitosan, stirring and dissolving, leaves standstill 12 hours.Get chloroform and the 6ml diox of 14ml respectively, mix homogeneously, takes 2.0g polylactic acid, stirring and dissolving, leaves standstill 12 hours.Chitosan and PLA solution are joined respectively in two syringes, with two-in-one syringe needle, two syringes are connected, pass to the voltage spinning of 25kV, be collected on the aluminium foil of ground connection, can obtain chitosan polylactic acid nano composite fiber thin film, fibre diameter is between 50-500nm.Get trifluoroacetic acid and the 1ml diox of 9ml respectively, mix homogeneously, takes 0.75g chitosan, stirring and dissolving, leaves standstill 12 hours.Get chloroform and the 1ml diox of 9ml respectively, mix homogeneously, takes 2.0g polylactic acid, stirring and dissolving, leaves standstill 12 hours.Chitosan and PLA solution are joined respectively in two syringes, with two-in-one syringe needle, two syringes are connected, pass to the voltage spinning of 12kV, be collected on the aluminium foil of ground connection, can obtain chitosan polylactic acid micron composite fiber thin film, fibre diameter is between 1-10 μm.The nanometer obtained by electrostatic spinning and micrometer fibers film are immersed in the solvent removed in the sodium hydroxide solution of 0.5mol/L and do not volatilize, and wash air-dry.Take 2.4g and 0.6g nanometer and micron chitosan PLA Composite Fiber film respectively, short fiber is cut in 100ml water high speed, divide sample in weighing botle, freeze forming at-18 DEG C, can obtain nano micron fibre chitosan polylactic acid complex three-dimensional support in freeze dryer after lyophilizing.
Claims (5)
1. nano micron fibre chitosan polylactic acid compound rest and preparation method thereof, it is characterized in that distinguishing electrostatic spinning nano and micron chitosan PLA Composite Fiber film under certain condition, in the sodium hydroxide solution of 0.5mol/L, remove the solvent do not volatilized and wash air-dry after, according to a certain ratio nanometer and micron chitosan PLA Composite Fiber film are cut into short fiber in water high speed, after dividing sample, freeze forming, obtains nano micron fibre chitosan polylactic acid complex three-dimensional support through lyophilization.
2. nano micron fibre chitosan polylactic acid compound rest according to claim 1 and preparation method thereof, it is characterized in that used chitosan molecule amount is between 20 ~ 400,000, polylactic acid molecule amount is between 5 ~ 100,000.
3. nano micron fibre chitosan polylactic acid compound rest according to claim 1 and preparation method thereof, when it is characterized in that Electrospun nano-fibers film, chitosan concentration is 5.0%, solvent is that trifluoroacetic acid is with the mixture of diox, volume ratio is 70:30, and PLA concentration is 10.0%, and solvent is that chloroform is with the mixture of diox, volume ratio is 70:30, and spinning voltage is 20 ~ 25kV.
4. nano micron fibre chitosan polylactic acid compound rest according to claim 1 and preparation method thereof, when it is characterized in that electrostatic spinning micron chitin fiber film, chitosan concentration is 7.5%, solvent is that trifluoroacetic acid is with the mixture of diox, volume ratio is 90:10, and PLA concentration is 20.0%, and solvent is that chloroform is with the mixture of diox, volume ratio is 90:10, and spinning voltage is 12 ~ 15kV.
5. nano micron fibre chitosan polylactic acid compound rest according to claim 1 and preparation method thereof, it is characterized in that the mass ratio of Nano chitosan PLA Composite Fiber and micron chitosan PLA Composite Fiber is between 20:80 to 80:20, the total concentration of chitosan polylactic acid nano micrometer fibers in water is between 1 ~ 3%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475797A (en) * | 2016-06-08 | 2017-12-15 | 南京理工大学 | A kind of magnetic Nano chopped fiber and preparation method thereof |
| WO2018227587A1 (en) * | 2017-06-16 | 2018-12-20 | 深圳大学 | Method for preparing anti-smog nonwoven fabric |
| CN109381747A (en) * | 2017-08-10 | 2019-02-26 | 沙冰娟 | A kind of preparation method of polylactic acid-chitose fiber scaffold material |
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| CN104888278A (en) * | 2015-05-20 | 2015-09-09 | 东华大学 | Nanometer/micrometer fiber three-dimensional porous structure support material and preparation and application of support material |
| CN105107022A (en) * | 2015-09-21 | 2015-12-02 | 东华大学 | Preparation method for nanofiber porous scaffold having compression elasticity in wet state |
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| WO2014160019A1 (en) * | 2013-03-14 | 2014-10-02 | Lifenet Health | Aligned fiber and method of use thereof |
| CN103285424A (en) * | 2013-05-27 | 2013-09-11 | 东华大学 | Three-dimensional fiber-based aerogel tissue engineering scaffold and preparation method thereof |
| CN103691005A (en) * | 2013-12-24 | 2014-04-02 | 华东交通大学 | Micro-nanofiber tissue engineering scaffold and preparation method thereof |
| CN104888278A (en) * | 2015-05-20 | 2015-09-09 | 东华大学 | Nanometer/micrometer fiber three-dimensional porous structure support material and preparation and application of support material |
| CN105107022A (en) * | 2015-09-21 | 2015-12-02 | 东华大学 | Preparation method for nanofiber porous scaffold having compression elasticity in wet state |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475797A (en) * | 2016-06-08 | 2017-12-15 | 南京理工大学 | A kind of magnetic Nano chopped fiber and preparation method thereof |
| WO2018227587A1 (en) * | 2017-06-16 | 2018-12-20 | 深圳大学 | Method for preparing anti-smog nonwoven fabric |
| CN109381747A (en) * | 2017-08-10 | 2019-02-26 | 沙冰娟 | A kind of preparation method of polylactic acid-chitose fiber scaffold material |
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