CN105532462A - Polygonum multiflorum bud proliferation medium and preparing method thereof - Google Patents
Polygonum multiflorum bud proliferation medium and preparing method thereof Download PDFInfo
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- CN105532462A CN105532462A CN201610011321.0A CN201610011321A CN105532462A CN 105532462 A CN105532462 A CN 105532462A CN 201610011321 A CN201610011321 A CN 201610011321A CN 105532462 A CN105532462 A CN 105532462A
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- fleece
- medium
- flower root
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- shoot propagation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
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- Botany (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a polygonum multiflorum bud proliferation medium and belongs to the technical field of polygonum multiflorum bud tissue culture. An MS medium serves as a basic medium of the medium, and 6-BA (6-benzylamino adenine), IBA (indolebutyric acid), a samarium-containing soluble compound, agar powder and white granulated sugar are added. Samarium is added in the medium, the polygonum multiflorum bud proliferation coefficient can be greatly increased, and proliferation time is shortened.
Description
Technical field
The present invention relates to fleece-flower root technical field of tissue culture, particularly relate to a kind of fleece-flower root Shoot propagation medium, also relate to a kind of fleece-flower root Shoot propagation medium compound method.
Background technology
The fleece-flower root, be that the polygonaceae fleece-flower root belongs to perennial winding liane, block root manure is thick, oblong, pitchy, and its block root is used as medicine, and can calm the nerves, nourish blood, active, disappear carbuncle, be common traditional Chinese medicine.
Current tissue cultures is one of conventional means of fleece-flower root artificial rapid propagation, but in existing tissue culture technique, fleece-flower root Shoot propagation coefficient is not high, generation time is long, is more and more difficult to the demand meeting market.
Summary of the invention
There are the problems referred to above based on prior art, the invention provides a kind of fleece-flower root Shoot propagation medium, it is medium based on MS medium, and is added with 6-BA(6-benzyl aminoadenine), IBA(indolebutyric acid), containing samarium soluble compound, agar powder and white granulated sugar.Medium in the present invention with the addition of samarium element, greatly can improve fleece-flower root Shoot propagation coefficient, shortens generation time.
A kind of fleece-flower root Shoot propagation medium, medium based on MS medium, and be added with 6-BA(6-benzyl aminoadenine), IBA(indolebutyric acid), containing samarium soluble compound, agar powder and white granulated sugar.
The concentration of 6-BA (6-benzyl aminoadenine) is 0.5-1.5mg/L, IBA(indolebutyric acid) concentration be 0.1-0.5mg/L, concentration containing samarium soluble compound is 0.1-0.2mM, and the concentration of agar powder is 8 ~ 11g/L, and the concentration of sucrose is 25 ~ 35g/L.
The concentration of 6-BA (6-benzyl aminoadenine) is 0.8-1.2mg/L, IBA(indolebutyric acid) concentration be 0.2-0.4mg/L, concentration containing samarium soluble compound is 0.12-0.16mM, and the concentration of agar powder is 9 ~ 10g/L, and the concentration of sucrose is 28 ~ 33g/L.
The pH value of described fleece-flower root blade inducing culture is 6.3 ~ 6.9.
The pH value of described fleece-flower root blade inducing culture is 6.7, and neutral environment is applicable to fleece-flower root Shoot propagation, can improve growth coefficient.
Be samaric nitrate containing samarium soluble compound, can also be samarium oxide or samarium trichloride, samarium element be a kind of rare earth element, has the effect of plant growth regulator, can stimulate Promoting plant growth, is usually used in the Quality and yield improving crops.
A compound method for fleece-flower root Shoot propagation medium, it comprises the following steps:
Step S10 dissolves preparation medium: dissolve MS basal medium each composition with distilled water, and add 6-BA(6-benzyl aminoadenine), IBA(indolebutyric acid), containing samarium soluble compound, agar powder and white granulated sugar;
Step S20 high-temperature sterilization: the medium prepared by step S10 is put in high-pressure sterilizing pot, in 120 ~ 121 DEG C of high-temperature sterilizations 20 ~ 21 minutes, cooled and solidified.
Step S10 also comprises step S11 adjust ph: use HCL and KOH titration to regulate Medium's PH Value to 6.7.
Embodiment
Embodiment one: preparation fleece-flower root Shoot propagation medium.
Fleece-flower root Shoot propagation culture medium prescription according to the MS culture medium prescription shown in table 1 and table 2, prepares fleece-flower root Shoot propagation medium of the present invention.
Weigh in the balance and get each composition in MS medium and 6-BA(6-benzyl aminoadenine), IBA(indolebutyric acid), containing samarium soluble compound, agar powder and white granulated sugar, be placed in triangular flask, add appropriate distilled water, stirring and dissolving, and adjust ph to 6.7, be settled to 1000mL.Triangular flask is sealed, is placed in high-pressure sterilizing pot, in 121 DEG C of sterilizing 20min, is then placed in superclean bench and naturally cools, for subsequent use.
Being divided by medium when will be cooled to 50-60 DEG C is filled in tissue culture flasks, dries in the air cool, by tissue cultures bottle closure after it solidifies, for subsequent use.
The composition of table 1MS medium and consumption thereof.
The composition of table 2 fleece-flower root Shoot propagation medium and consumption thereof.
Embodiment two: the test of fleece-flower root Shoot propagation medium of the present invention
Fleece-flower root Shoot propagation culture medium prescription according to the MS culture medium prescription shown in table 1 and table 3, with reference to the medium preparation method described in embodiment one, prepares the fleece-flower root Shoot propagation medium of each experimental group, control group respectively.
Table 3 fleece-flower root Shoot propagation medium.
Choose in superclean bench growth normal, without the fleece-flower root Multiple Buds of brownization, Multiple Buds is taken out, with aseptic operation cutter, Multiple Buds is divided into simple bud, being inoculated in by simple bud in the medium of each experimental group, control group shown in table 3, is then that 12h illumination/12h is dark, intensity of illumination is 2000lux, temperature is cultivated under being the condition of 25 ± 2 DEG C in light dark period.Cultivate 30 days statistics propagation fleece-flower root bud value-added coefficient, melting brown rate afterwards.
Table 4 fleece-flower root Shoot propagation medium test result.
Note: value-added coefficient=(after increment the dead bud number of bud number-brownization)/inoculation bud number.
From the result of the test of table 4, adopt fleece-flower root Shoot propagation medium provided by the invention growth coefficient can be brought up to more than 5, although to compare with experimental group from control group 3 simultaneously and can find out that samarium element can play the effect of certain coordinate plant growth, but DeGrain, samarium element more acts on and is coordinate plant growth state, the mutual work of growth promoting effects element and the basic element of cell division, thus promote fleece-flower root Shoot propagation.In addition because tissue cultures shoot proliferation is more, mutation probability is larger, is ensureing under the prerequisite do not made a variation, is improving proliferate efficiency and can substantially reduce the increment time.
Claims (8)
1. a fleece-flower root Shoot propagation medium, is characterized in that, medium based on MS medium, and is added with 6-BA(6-benzyl aminoadenine), IBA(indolebutyric acid), containing samarium soluble compound, agar powder and white granulated sugar.
2. a kind of fleece-flower root Shoot propagation medium according to claim 1, it is characterized in that, the concentration of 6-BA (6-benzyl aminoadenine) is 0.5-1.5mg/L, IBA(indolebutyric acid) concentration be 0.1-0.5mg/L, concentration containing samarium soluble compound is 0.1-0.2mM, the concentration of agar powder is 8 ~ 11g/L, and the concentration of sucrose is 25 ~ 35g/L.
3. a kind of fleece-flower root Shoot propagation medium according to claim 2, it is characterized in that, the concentration of 6-BA (6-benzyl aminoadenine) is 0.8-1.2mg/L, IBA(indolebutyric acid) concentration be 0.2-0.4mg/L, concentration containing samarium soluble compound is 0.12-0.16mM, the concentration of agar powder is 9 ~ 10g/L, and the concentration of sucrose is 28 ~ 33g/L.
4. according to claims 1 to 3 one of them described in a kind of fleece-flower root Shoot propagation medium, it is characterized in that, the pH value of described fleece-flower root blade inducing culture is 6.3 ~ 6.9.
5. described a kind of fleece-flower root Shoot propagation medium according to claim 4, it is characterized in that, the pH value of described fleece-flower root blade inducing culture is 6.7.
6. according to claims 1 to 3 one of them described in a kind of fleece-flower root Shoot propagation medium, it is characterized in that, described is samaric nitrate containing samarium soluble compound, can also be samarium oxide or samarium trichloride.
7. a compound method for fleece-flower root Shoot propagation medium, is characterized in that, it comprises the following steps:
Step S10 dissolves preparation medium, dissolves MS basal medium each composition, and add 6-BA(6-benzyl aminoadenine with distilled water), IBA(indolebutyric acid), containing samarium soluble compound, agar powder and white granulated sugar;
Step S20 high-temperature sterilization, the medium prepared by step S10 is put in high-pressure sterilizing pot, in 120 ~ 121 DEG C of high-temperature sterilizations 20 ~ 21 minutes, cooled and solidified.
8. the compound method of a kind of fleece-flower root Shoot propagation medium according to claim 7, it is characterized in that, described step S10 also comprises step S11 adjust ph, uses HCL and KOH titration to regulate Medium's PH Value to 6.7.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610011321.0A CN105532462A (en) | 2016-01-09 | 2016-01-09 | Polygonum multiflorum bud proliferation medium and preparing method thereof |
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| CN201610011321.0A CN105532462A (en) | 2016-01-09 | 2016-01-09 | Polygonum multiflorum bud proliferation medium and preparing method thereof |
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| CN201610011321.0A Pending CN105532462A (en) | 2016-01-09 | 2016-01-09 | Polygonum multiflorum bud proliferation medium and preparing method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108605811A (en) * | 2016-12-10 | 2018-10-02 | 江苏小潘园生态农业科技发展有限公司 | A kind of fleece-flower root culture medium |
| CN116114706A (en) * | 2022-11-30 | 2023-05-16 | 华中农业大学 | Application of samarium nitrate in relieving high-temperature stress of rice |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1391796A (en) * | 2001-06-15 | 2003-01-22 | 中国科学院化工冶金研究所 | Method for preventing plant indefinite bud and cultivating seedling in glass container |
| CN1934933A (en) * | 2006-10-08 | 2007-03-28 | 南京林业大学 | Tissue culture quick-breeding method for polygonum multiflorum |
| CN102870675A (en) * | 2012-09-26 | 2013-01-16 | 李斌 | Method for tissue culture of sprout |
| CN105010140A (en) * | 2015-07-07 | 2015-11-04 | 南京师范大学 | Culture media for promoting induction and rooting of cluster buds of dendrobium candidum and culture method by using rare earth elements |
-
2016
- 2016-01-09 CN CN201610011321.0A patent/CN105532462A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1391796A (en) * | 2001-06-15 | 2003-01-22 | 中国科学院化工冶金研究所 | Method for preventing plant indefinite bud and cultivating seedling in glass container |
| CN1934933A (en) * | 2006-10-08 | 2007-03-28 | 南京林业大学 | Tissue culture quick-breeding method for polygonum multiflorum |
| CN102870675A (en) * | 2012-09-26 | 2013-01-16 | 李斌 | Method for tissue culture of sprout |
| CN105010140A (en) * | 2015-07-07 | 2015-11-04 | 南京师范大学 | Culture media for promoting induction and rooting of cluster buds of dendrobium candidum and culture method by using rare earth elements |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108605811A (en) * | 2016-12-10 | 2018-10-02 | 江苏小潘园生态农业科技发展有限公司 | A kind of fleece-flower root culture medium |
| CN116114706A (en) * | 2022-11-30 | 2023-05-16 | 华中农业大学 | Application of samarium nitrate in relieving high-temperature stress of rice |
| CN116114706B (en) * | 2022-11-30 | 2023-11-28 | 华中农业大学 | Application of samarium nitrate in relieving high-temperature stress of rice |
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Application publication date: 20160504 |