CN105527234A - Method for detecting content of vitamin C in fruits and vegetables - Google Patents
Method for detecting content of vitamin C in fruits and vegetables Download PDFInfo
- Publication number
- CN105527234A CN105527234A CN201510992542.6A CN201510992542A CN105527234A CN 105527234 A CN105527234 A CN 105527234A CN 201510992542 A CN201510992542 A CN 201510992542A CN 105527234 A CN105527234 A CN 105527234A
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- solution
- centrifuge tube
- vitamin
- standard solution
- fruits
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Abstract
The invention provides a method for detecting the content of vitamin C in fruits and vegetables. The method comprises the following steps: triturating a fruit or vegetable product to be detected, taking the obtained juice to obtain a standard solution, and disposing the standard solution in a centrifuge tube; adding a Ag<+> standard solution to the centrifuge tube; adding clear water to the centrifuge tube, shaking up the obtained solution, and disposing the centrifuge tube in a shady place at room temperature; centrifuging the solution, removing the obtained supernatant, and washing the obtained precipitate with ultrapure water; dissolving the precipitate with concentrated nitric acid according to a ratio of 1:1, transferring the obtained solution to a volumetric flask, and adding water to dilute the solution; and injecting air-acetylene flame, determining the absorbance of the solution, and drawing a standard curve with the concentrations of the standard solution to be detected as abscissa and measured absorbance values as ordinate. The method has the advantages of stable determination result, simple operation and wide detection range, and can be widely used to rapidly detect vitamin C in foods.
Description
Technical field
The present invention relates to a kind of detection technique of Fast Measurement vitamin C component in food, especially relate to Vitamin C content detection method in a kind of fruits and vegetables.
Background technology
Along with the raising of people's quality of life, people more and more focus on dietary structure and healthy.Vitamin C is one of vitamin of wanting of body weight for humans, can not synthesize, so meals must be relied on to supply in human body.The human body C that is deficient in vitamin can cause various diseases, as scurvy, so ascorbic content detection also becomes very important in fruit and vegetable food.Prior art ascorbic assay method Main Basis national standard method fluorescence method, to utilize in sample reduced form ascorbic acid through activated carbon oxidation for after hydroascorbic acid, the quinoxaline generating fluorescence is reacted with o-phenylenediamine (OPDA), its fluorescence intensity is directly proportional under certain condition to the concentration of hydroascorbic acid, measures ascorbic amount in food with this.This method operation is very loaded down with trivial details, and detection speed is slow, and detection efficiency is low.
Summary of the invention
For solving the problems of the technologies described above, the technical solution used in the present invention is: Vitamin C content detection method in a kind of fruits and vegetables, comprises the following steps:
(1) fruit and vegetable food to be detected is smashed to pieces get juice, obtain standard solution;
(2) get standard solution 1-2 part of step (1), be placed in centrifuge tube;
(3) in centrifuge tube, 1-2 part Ag is added again
+standard solution;
(4) in centrifuge tube, add clear water 1-2 part again, shake up, at room temperature lucifuge places 30-40 minute;
(5) solution after step (4) being completed carries out centrifuging, removes supernatant, then precipitates 3-4 time with 2-3 part milli-Q water;
(6), after red fuming nitric acid (RFNA) 2-3 part dissolution precipitation of the solution 1:1 after step (5) being completed, be moved in volumetric flask, add water and dilute;
(7) spray into Air-acetylene Flame and measure its absorbance, with the concentration of standard solution to be detected for horizontal ordinate, with the absorbance recorded for ordinate drawing standard curve, the Vitamin C content in fruit and vegetable food to be detected can be obtained.
Further, the standard solution that described step (1) obtains utilizes the citric acid of 1-2 part 2-3% to mix as solvent.
Further, described centrifuge tube is brown centrifuge tube.
The advantage that the present invention has and good effect are: the present invention utilizes citric acid solution to carry out preparing standard solution, slow down the speed that vitamin C is oxidized, eliminate the interference of certain extraneous factor simultaneously, make measurement result more stable; And the advantages such as simple to operate, sensing range is wide, the quick detection that can be widely used in food vitamins C that the present invention has.
Embodiment
Embodiment:
Vitamin C content detection method in a kind of fruits and vegetables, comprises the following steps:
(1) fruit and vegetable food to be detected is smashed to pieces get juice, mix as solvent with the citric acid of 1 part 2%, obtain standard solution;
(2) get the standard solution 2 parts of step (1), be placed in brown centrifuge tube;
(3) in brown centrifuge tube, 2 parts of Ag are added again
+standard solution;
(4) in brown centrifuge tube, add 1 part, clear water again, shake up, at room temperature lucifuge places 35 minutes;
(5) solution after step (4) being completed carries out centrifuging, removes supernatant, then precipitates 3 times with 2 parts of milli-Q water;
(6), after the red fuming nitric acid (RFNA) 3 parts of dissolution precipitations of the solution 1:1 after step (5) being completed, be moved in volumetric flask, add water and dilute;
(7) spray into Air-acetylene Flame and measure its absorbance, with the concentration of standard solution to be detected for horizontal ordinate, with the absorbance recorded for ordinate drawing standard curve, the Vitamin C content in fruit and vegetable food to be detected can be obtained.
It is emphasized that; embodiment of the present invention is illustrative; instead of it is determinate; therefore the present invention is not limited to the embodiment described in embodiment; every other embodiments drawn by those skilled in the art's technical scheme according to the present invention, belong to the scope of protection of the invention equally.
Claims (3)
1. a Vitamin C content detection method in fruits and vegetables, is characterized in that: comprise the following steps:
(1) fruit and vegetable food to be detected is smashed to pieces get juice, obtain standard solution;
(2) get standard solution 1-2 part of step (1), be placed in centrifuge tube;
(3) in centrifuge tube, 1-2 part Ag is added again
+standard solution;
(4) in centrifuge tube, add clear water 1-2 part again, shake up, at room temperature lucifuge places 30-40 minute;
(5) solution after step (4) being completed carries out centrifuging, removes supernatant, then precipitates 3-4 time with 2-3 part milli-Q water;
(6), after red fuming nitric acid (RFNA) 2-3 part dissolution precipitation of the solution 1:1 after step (5) being completed, be moved in volumetric flask, add water and dilute;
(7) spray into Air-acetylene Flame and measure its absorbance, with the concentration of standard solution to be detected for horizontal ordinate, with the absorbance recorded for ordinate drawing standard curve, the Vitamin C content in fruit and vegetable food to be detected can be obtained.
2. Vitamin C content detection method in fruits and vegetables according to claim 1, is characterized in that: the standard solution that described step (1) obtains utilizes the citric acid of 1-2 part 2-3% to mix as solvent.
3. Vitamin C content detection method in fruits and vegetables according to claim 1, is characterized in that: described centrifuge tube is brown centrifuge tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510992542.6A CN105527234A (en) | 2015-12-25 | 2015-12-25 | Method for detecting content of vitamin C in fruits and vegetables |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510992542.6A CN105527234A (en) | 2015-12-25 | 2015-12-25 | Method for detecting content of vitamin C in fruits and vegetables |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105527234A true CN105527234A (en) | 2016-04-27 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510992542.6A Pending CN105527234A (en) | 2015-12-25 | 2015-12-25 | Method for detecting content of vitamin C in fruits and vegetables |
Country Status (1)
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| CN (1) | CN105527234A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110208233A (en) * | 2019-06-09 | 2019-09-06 | 福建医科大学 | The method of nano cupric oxide enhancing fluoremetry ascorbic acid |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60227171A (en) * | 1984-04-25 | 1985-11-12 | Sogo Seibutsu Igaku Kenkyusho:Kk | Measuring method of glucose conjugated to hemoglobin |
| SU1608579A1 (en) * | 1988-04-04 | 1990-11-23 | Белгородский филиал Всесоюзного научно-исследовательского витаминного института | Method of quantitative determination of vitamins a,e,k3 in oil solutions containing carotene |
| CN103439274A (en) * | 2013-07-13 | 2013-12-11 | 中盐东兴盐化股份有限公司 | Method for detection of content of ferric ammonium citrate in table salt by spectrophotometry |
| CN103454233A (en) * | 2013-09-11 | 2013-12-18 | 浙江中一检测研究院股份有限公司 | Method for measuring sulfide in environment |
| CN103499475A (en) * | 2013-09-26 | 2014-01-08 | 苏州国环环境检测有限公司 | Method of measuring lead content in shredded squid by microwave digestion-atomic absorption spectroscopy |
-
2015
- 2015-12-25 CN CN201510992542.6A patent/CN105527234A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60227171A (en) * | 1984-04-25 | 1985-11-12 | Sogo Seibutsu Igaku Kenkyusho:Kk | Measuring method of glucose conjugated to hemoglobin |
| SU1608579A1 (en) * | 1988-04-04 | 1990-11-23 | Белгородский филиал Всесоюзного научно-исследовательского витаминного института | Method of quantitative determination of vitamins a,e,k3 in oil solutions containing carotene |
| CN103439274A (en) * | 2013-07-13 | 2013-12-11 | 中盐东兴盐化股份有限公司 | Method for detection of content of ferric ammonium citrate in table salt by spectrophotometry |
| CN103454233A (en) * | 2013-09-11 | 2013-12-18 | 浙江中一检测研究院股份有限公司 | Method for measuring sulfide in environment |
| CN103499475A (en) * | 2013-09-26 | 2014-01-08 | 苏州国环环境检测有限公司 | Method of measuring lead content in shredded squid by microwave digestion-atomic absorption spectroscopy |
Non-Patent Citations (1)
| Title |
|---|
| 董振明 等: "间接原子吸收法测定维生素C的含量", 《光谱学与光谱分析》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110208233A (en) * | 2019-06-09 | 2019-09-06 | 福建医科大学 | The method of nano cupric oxide enhancing fluoremetry ascorbic acid |
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Application publication date: 20160427 |
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