CN104492511A - Sulfur dioxide rapid detection reagent tube and detection method thereof - Google Patents
Sulfur dioxide rapid detection reagent tube and detection method thereof Download PDFInfo
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- CN104492511A CN104492511A CN201410810697.9A CN201410810697A CN104492511A CN 104492511 A CN104492511 A CN 104492511A CN 201410810697 A CN201410810697 A CN 201410810697A CN 104492511 A CN104492511 A CN 104492511A
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Abstract
The invention provides a detection reagent tube which is integrated, has good reagent sealing performance, is convenient to transport, continuously and rapidly combines the steps of multi-component reagent sampling, reaction, color comparison and the like, is safe, convenient, accurate and rapid. The detection reagent tube comprises a reagent cup and a reaction cup, wherein the reagent cup is connected with the reaction cup by a plastic tape; the reagent cup is divided into two cavities; the cavity 1 is internally filled with 500 microliters of solution A and the cavity 2 is internally filled with 500 microliters of solution B; the cavity 1 and the cavity 2 are sealed by an aluminum foil; the size of an opening formed in the bottom of a reagent packaging cup is smaller than or equal to the size of an opening formed in the top of a reaction colorimetric cuvette; the reaction cup is internally provided with two pricking needles; and the two pricking needles correspond to the cavity 1 and the cavity 2 of the reagent cup respectively. By virtue of the detection reagent tube, the limitation of a traditional laboratory detection instrument that the volume is large, the cost is high and an analysis method is complicated is effectively overcome, and the rapid detection requirements of supervision law-enforcing departments including quality supervision, industry and commerce, agriculture, sanitation and the like, and units including wholesale markets, supermarkets, restaurants, hotels and the like are met.
Description
Technical field
The present invention relates to a kind of detection Reagent Tube, and the detection method of content of sulfur dioxide, specifically, relate to a kind of content of sulfur dioxide quick detection reagent pipe, and the detection method of sulfur dioxide in food.
Background technology
In the process of manufacture of food, its distinctive color and luster is kept for making food, manufacturer often adds the color and luster control groups such as bleaching agent and divides, and the oxidation relying on it to have or reducing power suppress, destroy the variable color factor of food, food is faded or avoids brown stain occurs.In China's food service industry, use more component to be sulfur dioxide and sulphite, this kind of component has certain corrosiveness, and in food, excessive use can affect health.Especially sulfur dioxide, it is effectively bleached and antisepsis food, bleaching agent conventional in food processing and anticorrisive agent, but, Use out of range sulfur dioxide class additive, intestines and stomach will be damaged, cause feeling sick, vomiting, also can affect the absorption of calcium, therefore sulfur dioxide has strict consumption control as food additives, China in " food additives use sanitary standard " (GB2760-2011) to drinks, carbohydrate, native article, preserved fruit, in the food such as starch, the maximum use amount of bleaching agent has made regulation, in sulfur dioxide residue limits for 0.01-0.4g/L (kg) not etc.
Domestic the administration for industry and commerce and quality supervised department once repeatedly found individual traders and pedlars or some the food enterprises of some areas, in order to pursue its product, there is good appearance luster, or extend the packaging for foodstuff time limit, or for covering poor quality food, Misuse or Use out of range sulfur dioxide class additive in food.The poisoned food that retailers' bold production sulfur dioxide why like this exceeds standard, mainly because the detection means too backwardness of law enforcement agency, can not detect the sulfur dioxide composition in food on a large scale, fast.There is complex operation in traditional pararosaniline hydrochloridc colorimetric method, sensitivity is low, color development system is unstable, poor reproducibility, often causes testing result to occur comparatively big error.Therefore, on market, the method and technological means thereof that detect the content of sulfur dioxide in food are fast set up in active demand.
Summary of the invention
The invention discloses a kind of simple and easy to do sulfur dioxide detection method, invent coherent detection reagent and detector tube.
The present invention's reagent used is important component part of the present invention, mainly comprises:
Solution A: the two fluoroform sulfimide salt 0.5-1mL of formaldehyde 10-100mL+1-ethyl-3-methylimidazole, adds water and be settled to 1000mL.
B solution: pararosaniline hydrochloridc 0.1-2g+ hydrochloric acid 100-500mL, adds water and be settled to 1000mL.
The invention provides a kind of integrated, reagent sealing good, be convenient to transport, the steps such as multicomponent reagent pipetting volume, reaction, colorimetric are carried out continuously quick combination simultaneously, safer, easy, detect Reagent Tube accurately and rapidly.This detection Reagent Tube effectively overcomes the limitation that traditional test in laboratory instrument volume is comparatively large, cost is high, analytical method is complicated, can meet the quick detection demand of the units such as supervision law enforcement agency and wholesale market, supermarket, dining room, hotel etc. such as quality supervision, industry and commerce, agricultural, health.
This detection Reagent Tube comprises: reagent cup and reaction cup, reagent cup is connected by plastic tape with reaction cup, wherein reagent cup is divided into two chambeies, fill solution A 500uL in chamber 1, in chamber 2, fill B solution 500uL, by aluminium foil, chamber 1 and chamber 2 are sealed, the size of reagent cup bottom opening is less than or equal to the open-topped size of reaction cup, make the lower end of reagent cup can the inner chamber of intercalation reaction cup, be provided with two root thorn pins in reaction cup, two root thorn pins be corresponding with the chamber 1 of reagent cup and chamber 2 respectively.
The using method detecting Reagent Tube is as follows:
Sample detection and result judge
1) sample is added: get 1g testing sample and join in reaction cup, add 3mL water.
2) reagent adding: enter in reaction cup by reagent cup back-off, firmly press down, reagent is sealed aluminium foil and punctures by pricker, and reagent flows in reaction cup.
3) react: pressure-vaccum mixes, and room temperature leaves standstill 1min-10min.
4) result interpretation: with the content of sulfur dioxide in standard color comparison card colorimetric determination sample: reactant liquor is not containing sulfur dioxide in faint yellow interpret sample, in blue or bluish violet interpret sample containing sulfur dioxide, color is darker, and content of sulfur dioxide is higher.
The making of sulfur dioxide examination criteria colorimetric card:
Get the detection Reagent Tube that 12 embodiments 1 are obtained, respectively to adding 0.0 in reaction cup, 0.1,0.2,0.5,1.0,2.0,4.0,8.0,12.0,16.0,20.0,32.0mg/kg sulfur dioxide standard liquid 3.0mL.Enter in reaction cup by mentioned reagent cup back-off, firmly press down, reagent is sealed aluminium foil and punctures by pricker, and reagent flows in reaction cup.Pressure-vaccum mixes, and room temperature leaves standstill 5min, makes sulfur dioxide examination criteria colorimetric card.
The advantage that the present invention has and good effect are: adopt prefabricated integrated reagent, without the need to extemporaneous preparation, improve operating efficiency; Operating personnel are avoided directly to contact chemicals, safe and reliable; Without the need to using any instrument and auxiliary equipment, consume low; Highly sensitive, meet national examination criteria requirement; Naked eyes contrast color judges testing result, simple and clear; Detector tube deft design is unique, good looking appearance.Adopt the two fluoroform sulfimide salt of 1-ethyl-3-methylimidazole as SO
2absorbent, on the one hand effectively can absorb SO
2, make SO as stabilizing agent on the other hand
2stablize with the chromogenic reaction of pararosaniline hydrochloridc and formaldehyde, color clear, can not pass in time and change, and without the need to adding stabilizing agent in addition again, the detection limit of sulfur dioxide can be reduced to 0.3mg/kg, sensitiveer, favorable reproducibility, and testing result is stablized.
The invention belongs to technical field of food safety detection, be mainly used in the quick detection of content of sulfur dioxide in food samples.The applying unit mainly national law enforcement of checkout gear of the present invention, as the administration for industry and commerce, hygiene department, quality supervision department, commodity inspection and epidemic prevention department etc., food processing enterprises also have wilderness demand to this technology and product in control of product quality.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Fig. 2 is the use procedure schematic diagram that the present invention detects Reagent Tube.
In figure:
1-reagent cup 2-reaction cup 3-chamber 1
4-chamber 2 5-pricker
Detailed description of the invention
Embodiment 1
The compound method of solution is as follows:
Solution A: the two fluoroform sulfimide salt 0.5mL of formaldehyde 40mL+1-ethyl-3-methylimidazole, adds water and be settled to 1000mL.
B solution: pararosaniline hydrochloridc 0.5g+ hydrochloric acid 100mL, adds water and be settled to 1000mL.
As shown in Figure 1, a kind of Reagent Tube that detects comprises: reagent cup and reaction cup, reagent cup is connected by plastic tape with reaction cup, wherein reagent cup is divided into two chambeies, solution A 500uL is filled in chamber 1, B solution 500uL is filled in chamber 2, by aluminium foil, chamber 1 and chamber 2 are sealed, the size of reagent cup bottom opening is less than or equal to the open-topped size of reaction cup, make the lower end of reagent cup can the inner chamber of intercalation reaction cup, making reagent encapsulate cup lower end can the inner chamber of intercalation reaction cuvette, two root thorn pins are provided with in reaction cup, two root thorn pins are corresponding with the chamber 1 of reagent cup and chamber 2 respectively.
Embodiment 2
The making of sulfur dioxide examination criteria colorimetric card:
Get the detection Reagent Tube that 12 embodiments 1 are obtained, respectively to adding 0.0 in reaction cup, 0.1,0.2,0.5,1.0,2.0,4.0,8.0,12.0,16.0,20.0,32.0mg/kg sulfur dioxide standard liquid 3.0mL.Enter in reaction cup by reagent cup back-off, firmly press down, reagent is sealed aluminium foil and punctures by pricker, and reagent flows in reaction cup.Pressure-vaccum mixes, and room temperature leaves standstill 5min, makes sulfur dioxide examination criteria colorimetric card.
Embodiment 3
Application examples: the white granulated sugar sample choosing 10 manufacturer production, measures its content of sulfur dioxide by this law and National Standard Method respectively.
Add sample: get 1g testing sample and join in reaction cup, add 3mL distilled water.
Reagent adding: enter in reaction cup by the reagent cup back-off of embodiment 1, firmly press down, reagent is sealed aluminium foil and punctures by pricker, reagent flows in reaction cup.
Reaction: pressure-vaccum mixes, and room temperature leaves standstill 5min.
Testing result sees table 1, and this law can detect the content range of sulfur dioxide substantially, and its detection limit can reach 0.3mg/kg, can detect the content of sulfur dioxide fast, as needed accurately to detect, can use absorption photometric instrument auxiliary detection.
| Sample | This law (mg/kg) | National Standard Method (mg/kg) |
| 1 | 24-32 | 29.80 |
| 2 | 12-24 | 22.03 |
| 3 | 12-24 | 18.93 |
| 4 | 12-24 | 19.08 |
| 5 | 24-32 | 30.96 |
| 6 | 32-60 | 33.85 |
| 7 | 24-32 | 26.74 |
| 8 | 3-6 | 5.08 |
| 9 | 6-12 | 10.63 |
| 10 | 12-24 | 13.95 |
Embodiment 4
The compound method of solution is as follows:
Solution A: the two fluoroform sulfimide salt 2mL of formaldehyde 50mL+1-ethyl-3-methylimidazole, adds water and be settled to 1000mL.
B solution: pararosaniline hydrochloridc 2g+ hydrochloric acid 200mL, adds water and be settled to 1000mL.
Detect in the chamber 1 of Reagent Tube and fill solution A 500uL, fill B solution 500uL in chamber 2 and obtain detecting Reagent Tube.
Embodiment 5
The making of sulfur dioxide examination criteria colorimetric card:
Get the detection Reagent Tube that 12 embodiments 4 are obtained, respectively to adding 0.0 in reaction cup, 0.1,0.2,0.5,1.0,2.0,4.0,8.0,12.0,16.0,20.0,32.0mg/kg sulfur dioxide standard liquid 3.0mL.Enter in reaction cup by reagent cup back-off, firmly press down, reagent is sealed aluminium foil and punctures by pricker, and reagent flows in reaction cup.Pressure-vaccum mixes, and room temperature leaves standstill 5min, makes sulfur dioxide examination criteria colorimetric card.
Embodiment 6
Application examples: detect bean vermicelli and bamboo shoots containing amount of sulfur dioxide
Add sample: get 1g testing sample and join in reaction cup, add 3mL distilled water.
Reagent adding: enter in reaction cup by the reagent cup back-off of embodiment 4, firmly press down, reagent is sealed aluminium foil and punctures by pricker, reagent flows in reaction cup.
Reaction: pressure-vaccum mixes, and room temperature leaves standstill 5min.
Detect that containing amount of sulfur dioxide in bean vermicelli is 6-12mg/kg, containing amount of sulfur dioxide in bamboo shoots is 24-32mg/kg.
Embodiment 7
Replica test is tested
Get the sulfur dioxide standard liquid 3.0mL of 5 parts of 4.0mg/kg in the reaction cup of embodiment 1, enter in reaction cup by reagent cup back-off, firmly press down, reagent is sealed aluminium foil and punctures by pricker, and reagent flows in reaction cup.Pressure-vaccum mixes, and room temperature leaves standstill 5min.
Colour developing result indifference, solid colour, can draw thus and adopt method of the present invention to detect sulfur dioxide, color development system is stablized, favorable reproducibility.
Claims (4)
1. a sulfur dioxide quick detection reagent pipe, comprise: reagent cup and reaction cup, reagent cup is connected by plastic tape with reaction cup, wherein reagent cup is divided into two chambeies, solution A 500uL is filled in chamber 1, B solution 500uL is filled in chamber 2, by aluminium foil, chamber 1 and chamber 2 are sealed, the size of reagent cup bottom opening is less than or equal to the open-topped size of reaction cup, make the lower end of reagent cup can the inner chamber of intercalation reaction cup, two root thorn pins are provided with in reaction cup, two root thorn pins are corresponding with the chamber 1 of reagent cup and chamber 2 respectively, wherein solution A: the two fluoroform sulfimide salt 0.5-1mL of formaldehyde 10-100mL+1-ethyl-3-methylimidazole, add water and be settled to 1000mL, B solution: pararosaniline hydrochloridc 0.1-2g+ hydrochloric acid 100-500mL, adds water and be settled to 1000mL.
2. sulfur dioxide quick detection reagent pipe, wherein solution A as claimed in claim 1: the two fluoroform sulfimide salt 0.5mL of formaldehyde 40mL+1-ethyl-3-methylimidazole, adds water and be settled to 1000mL; B solution: pararosaniline hydrochloridc 0.5g+ hydrochloric acid 100mL, adds water and be settled to 1000mL.
3. sulfur dioxide quick detection reagent pipe as claimed in claim 2, also comprises standard color comparison card, adopts and obtain with the following method:
Get 12 detection Reagent Tubes according to claim 2, respectively to adding 0.0 in reaction cup, 0.1,0.2,0.5,1.0,2.0,4.0,8.0,12.0,16.0,20.0,32.0mg/kg sulfur dioxide standard liquid 3.0mL, mentioned reagent cup back-off is entered in reaction cup, firmly press down, reagent is sealed aluminium foil and punctures by pricker, and reagent flows in reaction cup, and pressure-vaccum mixes, room temperature leaves standstill 5min, makes sulfur dioxide examination criteria colorimetric card.
4. a sulfur dioxide method for quick, comprises the steps:
Add sample: get 1g testing sample and join in reaction cup according to claim 3, add 3mL water;
Reagent adding: reagent cup back-off is entered in reaction cup, firmly presses down, reagent is sealed aluminium foil and punctures by pricker, and reagent flows in reaction cup;
Reaction: pressure-vaccum mixes, and room temperature leaves standstill 1min-10min;
Result interpretation: with the content of sulfur dioxide in standard color comparison card colorimetric determination sample.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107045069A (en) * | 2017-03-27 | 2017-08-15 | 广州埃克森生物科技有限公司 | A kind of specific protein analyzer reaction cup handling system and its application method |
| CN107462575A (en) * | 2017-07-25 | 2017-12-12 | 广东省药品检验所（广东省药品质量研究所、广东省口岸药品检验所） | A kind of sulfur dioxide quick detection kit and detection method |
| CN107817358A (en) * | 2017-12-14 | 2018-03-20 | 江苏宝卫士生物科技有限公司 | A kind of diagnostic reagent detection means and its method |
| CN111965282A (en) * | 2020-08-18 | 2020-11-20 | 中国地质科学院矿产资源研究所 | Ultra-micro sulfur isotope analysis system and analysis method |
| CN112649420A (en) * | 2019-10-12 | 2021-04-13 | 苏州淳雨净化科技有限公司 | Special air comprehensive detection device for archives library |
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| CN102116744A (en) * | 2010-02-08 | 2011-07-06 | 力德力诺生物技术(北京)有限公司 | Device for detecting hydrogen peroxide and preparation method thereof |
| CN201926651U (en) * | 2010-08-18 | 2011-08-10 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Sulfur dioxide detection reagent kit |
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| CN2715146Y (en) * | 2004-07-26 | 2005-08-03 | 卢新 | Detection tube for sulfur dioxide in food |
| US20070045113A1 (en) * | 2005-08-29 | 2007-03-01 | Shinko Electric Industries Co., Ltd. | Solid electrolyte gas sensor |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107045069A (en) * | 2017-03-27 | 2017-08-15 | 广州埃克森生物科技有限公司 | A kind of specific protein analyzer reaction cup handling system and its application method |
| CN107462575A (en) * | 2017-07-25 | 2017-12-12 | 广东省药品检验所（广东省药品质量研究所、广东省口岸药品检验所） | A kind of sulfur dioxide quick detection kit and detection method |
| CN107817358A (en) * | 2017-12-14 | 2018-03-20 | 江苏宝卫士生物科技有限公司 | A kind of diagnostic reagent detection means and its method |
| CN107817358B (en) * | 2017-12-14 | 2023-11-07 | 江苏宝卫士生物科技有限公司 | Diagnostic reagent detection device and method thereof |
| CN112649420A (en) * | 2019-10-12 | 2021-04-13 | 苏州淳雨净化科技有限公司 | Special air comprehensive detection device for archives library |
| CN111965282A (en) * | 2020-08-18 | 2020-11-20 | 中国地质科学院矿产资源研究所 | Ultra-micro sulfur isotope analysis system and analysis method |
| CN111965282B (en) * | 2020-08-18 | 2023-08-29 | 中国地质科学院矿产资源研究所 | Ultra-trace sulfur isotope analysis system and analysis method |
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Effective date of registration: 20190627 Address after: 330000 No. 266 Huiren Avenue, Xiaolan Economic and Technological Development Zone, Nanchang City, Jiangxi Province Co-patentee after: Lidelinuo Biotechnology (Beijing) Co., Ltd. Patentee after: Nanchang Futailinuo Detection System Co. Ltd. Address before: 518102 Main Building 622, Taohuayuan Science and Technology Innovation Park, Tiegang Reservoir, Xixiang Street, Baoan City, Shenzhen City, Guangdong Province Patentee before: SHENZHEN LEADING NORM DETECTION APPLICATION SYSTEM CO., LTD. |