CN105497093A - Composition with auxiliary memory improvement function, preparation method of composition and capsule containing composition - Google Patents
Composition with auxiliary memory improvement function, preparation method of composition and capsule containing composition Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/286—Carthamus (distaff thistle)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/12—Ketones
- A61K31/122—Ketones having the oxygen directly attached to a ring, e.g. quinones, vitamin K1, anthralin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/66—Phosphorus compounds
- A61K31/683—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols
- A61K31/685—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols one of the hydroxy compounds having nitrogen atoms, e.g. phosphatidylserine, lecithin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
Abstract
The invention relates to a composition with an auxiliary memory improvement function. The composition is prepared from the following components in parts by weight: 40-60 parts of DHA spirulina oil, 30-50 parts of phosphatidylserine, 10-20 parts of safflower oil and 5-8 parts of astaxanthin oil. The composition has the technical advantage that the compatibility theory of the compound reflects the latest research results of active components and brain nutrients of the modern research; various unsaturated fatty acids of the brain nutrients and antioxidant ingredients of bioactive factors are reasonably combined; the defects of unilateral memory improvement products are overcome; overall regulation is strengthened from the basis conservation of brain cells; and a healthy brain is rebuilt.
Description
Technical field
The present invention relates to health product and preparation method thereof field, particularly relate to a kind of compositions, its preparation method and the capsule containing said composition with auxiliary improvement of memory power function.
Background technology
In the modern life, people's hypomnesis phenomenon is general.The forgetful commonly encountered diseases becoming city professional population, forgetful is the common multiple disease of old people, especially with cerebral blood supply insufficiency cause forgetful the most common.Population of adolescent is mainly manifested in and has a poor memory, and majority is owing to depending on computer, books etc. unduly, and excessive mental pressure, often smokes and drink, and the bad living habit such as frequent breakfast skipping causes.
The aging of human brain just started from 30 year, more obvious to the aging of brain time over fifty years old.With advancing age, brain there occurs some senile brains and changes old people in various degree, and serious meeting generation brain atrophy, therefore there will be hypomnesis.In addition, as cerebrovascular class disease, the exogenous loss of brain, nervous lesion class medicine, in meals, some composition etc. can cause the decline of memory function equally.Middle-aged and elderly people encephalopathy symptom reaches an advanced stage and shows as ischemic dementia, presenile dementia (AD) more.
The patients of senile dementia of current the Asian-Pacific area has 1,400 ten thousand people, and may be increased to 6,500 ten thousand people to the year two thousand fifty, wherein 40% from China.There is old dementia patients about 5,000,000 in current China, comprise presenile dementia and AD and the vascular dementia caused by cerebrovascular disease, in addition we are about to enter aging society, and numerous middle-aged and elderly people mass-brain health status causes anxiety, and the burden brought to society will be also huge.
Vascular dementia---the energy metabolism impairment caused by chronic cerebral ischemia, glucose utilization reduces, albumen resulting anomaly, and neurotransmitter changes, cholinoceptor lacks, cerebral white matter damage and neuron loss constitute the pathophysiological basis that chronic cerebral ischemia causes cognitive function to damage, and drastically influence the quality of life of old people, belong to one of infirmities of age, therefore, how to improve chronic cerebral ischemia symptom, delay it and occur, develop, become the problem that clinician pays close attention to.Oxidative stress is the important mechanisms of cerebral ischemia, confirm, neurocyte is very responsive to oxygen free radical injury, free radical chain reactions, the neuron etc. of primary challenge grey matter is rich in the cerebral tissue of lipid, make neuronal death, especially it can make the not serious neuron of initial ischemic injuries necrose.Mitochondrion is that in cell, oxygen-derived free radicals produces maximum places, also more subjects to radical damage and causes memory function reduction.Modern study shows dull-witted front pathological changes, comprises cerebral infarction, vascular hypertension, cerebral arteriosclerosis etc.Zhao Nan is firm etc. thinks, after cerebrovascular, the emotions such as the irritability of patient are stronger because of the puzzlement of disease, certainly will cause autonomic nervous dysfunction, endocrine dysfunction is lacked of proper care, and sympathetic excitability increases, Secretion of Catecholamine increases, blood pressure raises, and blood glucose raises, thus promotes arteriosclerosis, cause hemorheological change, more increase the weight of the long-term hypoxic-ischemic of brain cell, finally make brain cell produce irreversible infringement, occur that hypomnesis even causes vascular dementia.
Presenile dementia---there is serious degeneration in the choline system of presenile dementia patient cortex, causes learning and memory impairment and cognitive disorder, produces dementia symptom.When presenile dementia occurs, the cholinergic neuron of basal forebrain areas is lost, and causes the synthesis of acetylcholine, storage, release to reduce, thus to cause remembering with recognition function obstacle be main clinical manifestation.Radical damage is one of major reason causing AD.The lipid peroxidation of free radical and initiation thereof participates in the destruction that neuronic infringement oxygen-derived free radicals can cause neuron and glial cell biomembrane and subcellular structure and function.The oxygen-derived free radicals of a large amount of generation can cause mitochondrion DHA and mitochondrial membrane damage, and mitochondrion DHA damage occurs relevant with AD.
After people in middle age, namely the function and structure of brain changes gradually, and neurocyte and nerve fiber all can occur degenerative change, and brain weight alleviates, and cerebral blood flow also reduces about 17% than at an early age.Modern medicine study finds, the brain of people has highly plastic and great potential.With advancing age, knowledge, experience can be more and more abundanter, thus faculty of understanding and language competence can be improved, make old people exceed youngster, the prevention so old brain health problem can be kept healthy to the analysis of problem, inductive reasoning and judgement.
The brain of people is the maincenter producing thinking and consciousness, metabolism is very vigorous, brain function normally carry out the participation needing multiple nutrients material, in addition, when carrying out mental activity, brain cell needs a large amount of oxidations, if oxygen deficiency will affect the function and thinking of brain, if therefore memory to be improved normally can be run by supplementary brain necessary material, increase brain cell to the utilization of oxygen and supplementaryly can promote that the approach such as the material that brain metabolism product is got rid of realize.
Supplement nutrition brain key, refreshing machine is nourished, and brain siddhi can be normal.By improving cerebral cortex blood flow, making chronic cerebral ischemia anaerobic condition be improved, lowering the injury that the emergency reaction of brain causes, alleviating the damage of cerebral tissue, lower damage and apoptosis that brain nervous cell causes because of hypoxic-ischemic.
The principal item of the health food of auxiliary improvement of memory power in the market has: lipid, protein and amino acids, mineral and microelement kind, vitamins, Chinese herbal medicine class.US healthcare food association expert identification has the composition that memory is improved effect: five hydroxytryptamine mixture, antioxidant, novel Chroma-Pak, ginkgo agent, huperzine A, w-3 fatty acid, Phosphatidylserine, Herba Catharanthi Rosei extract.
Now, along with the development of era of knowledge-driven economy, about mankind's brain research brain health problem has become the focus of World Developed Countries concern.The U.S. proposes " brain 10 years ", the slogan that Japan's proposition " understands brain, protection brain, creation brain ", and Japanese brain research association proposes brain research to disclose brain function, overcome disease of brain and to develop artificial intelligence for pillar.The relevant authoritative department of China is also proposed the brand new ideas of " in preventing brain overworked, valuing brain health " to society.But the new results that existing market responds this theory are not satisfactory, especially the field comprehensively paying close attention to brain health in health diet is also very limited, majority controls disease with regard to disease, single goes solution auxiliary treatment disease of brain symptom, and the importance that comprehensive brain health care, brain nutrition aspect do not go response to prevent from basis, certainly relevant technical products service is also limited.
The technical scheme that the present invention solves, on the one hand for the construction of domestic brain health idea is done one's bit, also can bring glad tidings for the brain health of vast middle-aged and elderly people on the other hand, also can go out high economic benefit for social creativity simultaneously.
Summary of the invention
The object of the invention is the technical limitation for the oral series products of existing market brain-strengthening, provides a kind of comprehensive brain of supporting to protect brain safety, science, effectively, and has the compositions of auxiliary improvement of memory power, its preparation method and the capsule containing said composition.
By absorbing in right amount, specifically brain nutrition and regulatory factor promote human brain development and brain physiological metabolism, improve thinking activities, to reach the object of food supplementing the brain and foster brain, strengthen corticocerebral irritability and oxidative stress rejection ability, keep intelligence sensitive, delay brain aging, the mental memory function of service hoisting.
For reaching above object, the technical scheme that the present invention takes is:
There is a compositions for auxiliary improvement of memory power function, become by following component parts by weights array:
DHA algal oil 40-55 part, Phosphatidylserine 30-40 part, safflower oil 10-20 part, astaxanthin oil 5-8 part.
On the basis of such scheme, the described compositions with auxiliary improvement of memory power function, is become by following component parts by weights array:
DHA algal oil 45 parts, Phosphatidylserine 33 parts, safflower oil 14 parts, astaxanthin oil 8 parts.
In order to improve the effect of the present composition, described DHA algal oil is prepared from by following methods:
1) oil-rich microalgae pretreatment of raw material: add 2-4 times of water gaging (w/w) in rich oil schizochytrium dry powder, concentrated hydrochloric acid regulates PH to 1-2, stir, dissolve 40-50min, multigelation 2-3 time respectively at 30-40min freezing at-20 DEG C of temperature, 45 DEG C of temperature; Add initial algae dry powder 2 times of water gagings (w/w) again, 6MNaOH regulates PH to 12-13, and ultrasonic power is 120W, working time 20s, and interval time, 15s, worked 40-60 time, carried out ultrasonic vibration broken wall treatment under 4-10 DEG C of condition; And then add algae mud solution equal-volume pure water after ultrasonication process, wash 1-2 time, collect nonaqueous phase pretreatment and to wet algae mud, 50-60 DEG C of heat-wind circulate drying is no more than 10% to water content, obtains pretreatment algae mud siccative;
2) oils and fats extracts: to step 1) add 1-2 times amount 95% ethanol (w/v) in gained pretreatment algae mud siccative, after stirring, be transferred to supercritical CO rapidly
2in extraction column, extraction column ambient temperature controls at 35-45 DEG C, and in extraction column, Stress control is at 35-40MPa, when temperature, pressure are steady, and control CO
2flow velocity 1.6-1.8L/min starts extraction and recovery, and extraction time is 2-3h, collects extraction crude oil;
3) DHA enriching and purifying: to step 2) add 6-8 times amount 0.5MKOH-CH in gained crude oil
3oH solution (w/v), 60 DEG C of heated and stirred are to merging completely, add initial crude oil 2-3 times amount petroleum ether (w/v) again, leave standstill 15-20min after stirring, separated and collected lower floor filtrate, concentrated hydrochloric acid regulates PH to 1-2, add initial crude oil 6-8 times amount petroleum ether (w/v) afterwards, leave standstill 25-30min, collection ether layer solution decompression is concentrated into removes petroleum ether completely, obtains free oil; Then add rapidly free oil 10-15 times amount 12% carbamide alcoholic solution (w/v), after stirring under 0 DEG C of condition winterization 16-20h; Winterization terminates rear rapid sucking filtration, collects the filtrate being enriched unsaturated fatty acid DHA; Initial free oil 2-4 times amount pure water (w/v) is added in filtrate, concentrated hydrochloric acid regulates PH to neutral, add initial free oil 2-4 times amount petroleum ether (w/v), leave standstill and collect ether layer, with pure water washing 1-2 time, collect ether layer, add the anhydrous sodium sulfate (v/w) of ether layer liquor capacity 2-3%, carry out distilling under reduced pressure after the 2-4h that dewaters and collect enrichment DHA algal oil;
4) DHA algal oil finished product is refined: to step 3) add 0.02% vitamin E (w/v) in gained enrichment DHA algal oil, 0.015% sodium L-ascorbate-2-phosphate (w/v), winterization 8-12h at 4 DEG C of temperature, obtains light yellow refining DHA algal oil.
Have a preparation method for the compositions of auxiliary improvement of memory power function as above, concrete steps are as follows:
DHA algal oil, Phosphatidylserine, astaxanthin oil, safflower oil are fed intake in cryogenic high pressure homogenizer by component proportioning, in 15-20 DEG C, homogeneous under 30-35MPa condition.Homogeneous is twice homogenization, the homogeneous 5-8min of the homogeneous 10-15min of first time 6000-8000r/min, second time 8000-10000r/min.
In order to easy to use, compositions of the present invention can be prepared into capsule, wherein, and the above-mentioned content of compositions as capsule with auxiliary improvement of memory power function; Preparation method is:
Contents Fill is entered soft capsule, finally under low temperature (20-30 DEG C), low humidity (relative humidity 22%-28%) condition, is dried to water content at 6%-8%.
The invention has the advantages that:
Source and the character of complex compatibility composition of the present invention are as follows:
DHA algal oil: what scientific research showed to contribute to oxygen in brain cell from the W-3 unsaturated fatty acid (DHA, EPA) in bathypelagic fish oil enters the effusion with carbon dioxide, for the growth course improving brain, the brain diseasess such as prevention and therapy nerve and spirituality, the aging course delaying brain all has very important effect.The nutritionist research of Japan finds, human body is as lacked unsaturated fatty acid, and memory, ability of thinking are then difficult to be in normal condition.
DHA is mainly present in the component of PHOSPHATIDYL ETHANOLAMINE and Phosphatidylserine, and PHOSPHATIDYL ETHANOLAMINE is the main phospholipid composition of neuron membrane.Along with the growth at people's age, in brain, the corresponding minimizing of DHA content, thus can produce the phenomenons such as forgetful and dull-witted, and DHA can activate cranial nerve cell, eliminate interior free yl, contribute to postpone brain cell aging, keep brain cell activity, supplementary DHA can prevent and delaying senility course.Present research extensively approves that the plasticity of neuronic growth and synapse needs DHA, and this point from alzheimer disease (AD) disease, DHA content reduction rear cephalin can occur and be verified.
Research finds that fish oil source DHA is ethyl ester type, and microalgae source DHA is triglyceride type, and ethyl ester DHA is low many at the absorption of human body, metabolic efficiency, and also can produce oxidative pressure in metabolic process, brings adverse effect to human body.Relative to fish oil DHA, microalgae DHA is more easily absorbed by the body and metabolism, and bioavailability is high, and resource is stablized, and the vegetalitas source of pure natural, safety, not containing fishlike smell.DHA algal oil is extracted in microalgae, and its production technology, safety and the application in the field such as food, medicine, become focus at home.Supercritical extraction oil tech replaces traditional extractive with organic solvent mode gradually, improves the safety of goods.
Therefore, the present invention adopts modern extraction technique refine from oil-rich microalgae schizochytrium to go out algae oil DHA, uses as the main brain nutrition of complex soft capsule of the present invention and active component compatibility.
Phosphatidylserine: scientist isolates a kind of special acid phospholipids acyl serine in soybean phospholipid, Phosphatidylserine is mainly used in treating brain aging clinically, more causes everybody extensive concern in recent years with " brain specificity nutrient substance ".Experiment confirms, Phosphatidylserine can promote that oxygen and nutrient substance enter brain cell, stimulates the secretion of " dopamine " (a kind of very important male neurotransmitter) simultaneously.Also be verified improving such as the information transmission between the release of intracellular signaling, excretion vesicles and cell of the effect in brain function.In addition, Phosphatidylserine correlational study also finds, supplementary DHA can promote the accumulation of Phosphatidylserine, thus plays the function suppressing nerve cell death.
Research shows, can slow down the decline even reversing dull-witted or relevant to aging study, emotion, memory, attention etc. to the oral Phosphatidylserine of the patient suffering from AD; The Phosphatidylserine of debita spissitudo can strengthen the oxidation resistance of hippocampal neuron; illustrate that Phosphatidylserine can play the effect of neuroprotective cell on a cellular level, affirmed that Phosphatidylserine has the effect promoting study, hypermnesis and improve Senile disease further.
Therefore, select Phosphatidylserine as collaborative lifting active matter important in complex soft capsule compatibility composition of the present invention.
Astaxanthin: astaxanthin is the containing oxygen derivative of carotenoid, can quenching activity oxygen effectively, has higher nutrition and medical value.The graduate BrandonK.Harvey of US National teaches one section of up-to-date achievement in research and shows, astaxanthin can reduce in Adult Wistar rats effectively due to injury that ischemic brain injury causes, its mechanism of action is the response to oxidative stress stoping brain, reduce the release of glutamate, Glu, inhibited apoptosis.Domestic association area research shows, astaxanthin shows significant neuroprotective to ischemic mice, plays useful effect to improving the memory of vascular dementia disease; The damage of astaxanthin to the hippocampal neurons Antioxidant Enzyme Systems that acrylamide (a kind of neurotoxin of the property put aside) causes has certain repair, has protective effect to the hippocampal neurons mitochondrial injury under acrylamide effect.
Clinic study shows, astaxanthin has the effect of the cardiovascular and cerebrovascular diseases such as prevention of arterial sclerosis, coronary heart disease and ischemic brain injury.In addition study and also find, astaxanthin through the blood-brain barrier of animal, can produce antioxidant activity outward at barrier, can the effective nervous system disease such as prevention and therapy parkinsonism, thus can play slow down aging, protects the effect of brain cell.
Therefore, select astaxanthin as regulatory factor important in complex soft capsule compatibility composition of the present invention, the infringement of protecting or repairing brain cell to bring because of response to oxidative stress can be played.
Safflower oil: safflower oil is rich in the natural Vitamin E with vital activity, be that in all natural plants, content is the highest, its main component is linoleic acid in addition, is also that in natural known plants, content is the highest.Linoleic acid can synthesize other two kinds necessary unsaturated fatty acids (linolenic acid, arachidonic acid) in vivo, so linoleic acid is the most most important unsaturated fatty acid of human body, be the basic substance of life, participate in lipid metabolism, prevention cardiovascular and cerebrovascular disease.Modern study result shows, safflower oil can significantly improve the energy of mitochondrion anti-lipid peroxidation, thus improves Membrane Fluidity, swelling degree and atpase activity, plays a part to strengthen cellular anti-oxidant capacity by subcellular organelle mitochondria pathway.Experiment proves, long-term edible safflower seed oil has blood fat reducing, vessel softening, stabilizing blood pressure and promotes microcirculatory effect.
Therefore, the oil base compatibility composition in complex software capsule of the present invention selects safflower oil, can play the effect of basic regulating lipid metabolism, and other brain neutraceutical active ingredients are better played a role.
Technological merit of the present invention is:
The present invention is absorbed in the development and utilization of source, ocean natural nutrient and the functional activity factor.Draw the elite in wide life cradle, enrichment results from microalgae organism, utilize modem technology, extract the biomacromolecule unsaturated fatty acid algae oil DHA of high stability efficiently, be applied in the oral series products of complex of the present invention with the form of high biological value.
Because DHA is in the course of processing, easily oxidized, so with the addition of antioxidant in DHA algal oil prepared by the present invention, present composition compatibility composition also has superpower non-oxidizability in addition, therefore technical solution of the present invention can improve the stability of DHA greatly, improves its biological value.In addition, in DHA algal oil preparation technology of the present invention, adopt multigelation method to carry out breaking cellular wall pretreatment in conjunction with low temperature ultrasonic concussion method to oil-rich microalgae and slightly refine, a nearlyer step carries out low temperature supercritical extraction oils and fats, farthest maintain the stability of DHA molecular machinery, and improve its effective yield.The pretreatment of rich oil base material have employed the method for acid refining multigelation and the combination of alkali refining ultrasonic vibration, greatly can improve schizochytrium cell wall breaking degree on the one hand, also carried out preliminary oils and fats stripping simultaneously, promote subsequent technique grease extraction efficiency and efficiency, cryogenic conditions has also ensured stability, the effectively yield of target lubricant component on the other hand; The supercritical extraction technique of up-to-date maturation is have employed in total oils and fats refines, the crude oil obtained is not containing extractant, color and luster abnormal smells from the patient is good, owing to being low-temperature extraction, physiologically active and the effective content of target oils and fats can not be destroyed, efficiency is high, safety, and greatly reduce the use amount of organic solvent, industrialization is environmental protection more; In DHA enriching and purifying technique, have employed the method for urea clathration in conjunction with winterization, not only increase target product algae oil DHA content and purity, also farthest maintain the stability of effective ingredient, remain without auxiliary agent, ensured the quality of target product.
Complex compatibility theory of the present invention embodies and utilizes modern study active component and brain nutrient newest research results, reasonable compatibility is carried out to various brain nutrient unsaturated fatty acid and bioactie agent antioxidant content, overcome the defect of folk prescription Improving memory power product, from maintenance brain cell basis, the overall conditioning of strengthening, rebuilds healthy brain.
Detailed description of the invention
Embodiment 1
A preparation method for DHA algal oil, concrete steps are as follows:
1) microalgae pretreatment of raw material: add 2 times of water gagings (w/w) in schizochytrium dry powder, concentrated hydrochloric acid regulates PH to 1, stirs, dissolves 40min, multigelation 3 times respectively at 40min freezing at-20 DEG C of temperature, 45 DEG C of temperature; Add initial algae dry powder 2 times of water gagings (w/w) again, 6MNaOH regulates PH to 12, and ultrasonic power is 120W, working time 20s, and 15s, worked 40 times, carried out ultrasonic vibration broken wall treatment under 8 DEG C of conditions interval time; And then add algae mud solution equal-volume pure water after ultrasonication process, wash 2 times, collect nonaqueous phase pretreatment and to wet algae mud, 50 DEG C of heat-wind circulate dryings are no more than 10% to water content, obtain pretreatment algae mud siccative;
2) oils and fats extracts: to step 1) add 2 times amount 95% ethanol (w/v) in gained pretreatment algae mud siccative, after stirring, be transferred to supercritical CO rapidly
2in extraction column, extraction column ambient temperature controls at 38 DEG C, and in extraction column, Stress control is at 40MPa, when temperature, pressure are steady, and control CO
2flow velocity 1.6L/min starts extraction and recovery, and extraction time is 2h, collects extraction crude oil;
3) DHA enriching and purifying: to step 2) add 6 times amount 0.5MKOH-CH in gained crude oil
3oH solution (w/v), 60 DEG C of heated and stirred are to merging completely, add initial crude oil 2 times amount petroleum ether (w/v) again, leave standstill 15min after stirring, separated and collected lower floor filtrate, concentrated hydrochloric acid regulates PH to 1, add initial crude oil 8 times amount petroleum ether (w/v) again, leave standstill 25min, collection ether layer solution decompression is concentrated into removes petroleum ether completely, obtains free oil; Then add rapidly free oil 10 times amount 12% carbamide alcoholic solution (w/v), after stirring under 0 DEG C of condition winterization 18h; Winterization terminates rear rapid sucking filtration, collects the filtrate being enriched unsaturated fatty acid DHA; Initial free oil 4 times amount pure water (w/v) is added in filtrate, concentrated hydrochloric acid regulates PH to neutral, add initial free oil 3 times amount petroleum ether (w/v), leave standstill and collect ether layer, 2 times are washed with pure water, collect ether layer, add the anhydrous sodium sulfate (v/w) of ether layer liquor capacity 2%, carry out distilling under reduced pressure after the 4h that dewaters and collect enrichment DHA algal oil;
4) DHA algal oil finished product is refined: to step 3) add 0.02% vitamin E (w/v) and 0.015% sodium L-ascorbate-2-phosphate (w/v) in gained enrichment DHA algal oil, winterization 8h at 4 DEG C of temperature, obtains light yellow refining DHA algal oil.
Described refining DHA algal oil DHA content is the total extraction ratio of 86.2%, DHA is 15.3%, and color is more shallow, has the distinctive algae fragrance of microalgae, without fishlike smell.
Described schizochytrium dry powder buying is from Inner Mongolia Kingdomway Pharmaceutical Co., Ltd..
Embodiment 2
Have a DHA algal oil complex soft capsule for auxiliary improvement of memory power function, content is prepared from by the component of following parts by weight: DHA algal oil 45 parts, Phosphatidylserine 33 parts, safflower oil 14 parts, astaxanthin oil 8 parts.Described Phosphatidylserine buying is from Dou Shan group of Korea S (DoosanCorporation), described astaxanthin oil adopts purchased from American West Asia promise Imtech (cyanotech), and described safflower oil buying is from Handan morning twilight treasure oils and fats company limited.
Concrete preparation method is as follows:
1) DHA algal oil, Phosphatidylserine, astaxanthin oil, safflower oil are fed intake in cryogenic high pressure homogenizer by component proportioning, in 20 DEG C, under 35MPa condition homogeneous twice, the homogeneous 15min of first time 6000r/min, the homogeneous 8min of second time 8000r/min, obtains algae oil complex emulsion (capsule 's content).
2) by step 1) gained emulsion press 500mg/ grain specification metering fill soft capsule.Finally carry out low temperature (30 DEG C) low humidity (relative humidity 28%) be dried to dry ball water content 8%, pick ball and obtain the DHA algal oil complex soft capsule preparation that the present invention has auxiliary improvement of memory power.
Embodiment 3
Have a DHA algal oil complex soft capsule for auxiliary improvement of memory power function, content is prepared from by the component of following parts by weight: DHA algal oil 40 parts, Phosphatidylserine 40 parts, safflower oil 15 parts, astaxanthin oil 5 parts.Described Phosphatidylserine buying is from Dou Shan group of Korea S (DoosanCorporation), described astaxanthin oil adopts purchased from American West Asia promise Imtech (cyanotech), and described safflower oil buying is from Handan morning twilight treasure oils and fats company limited.
Concrete preparation method is as follows:
1) DHA algal oil, Phosphatidylserine, astaxanthin oil, safflower oil are fed intake in cryogenic high pressure homogenizer by component proportioning, in 20 DEG C, under 35MPa condition homogeneous twice, the homogeneous 12min of first time 7000r/min, the homogeneous 6min of second time 9000r/min, obtains algae oil complex emulsion (capsule 's content).
2) by step 1) gained emulsion press 600mg/ grain specification metering fill soft capsule.Finally carry out low temperature (28 DEG C) low humidity (relative humidity 26%) be dried to dry ball water content 8%, pick ball and obtain the DHA algal oil complex soft capsule preparation that the present invention has auxiliary improvement of memory power.
Embodiment 4
Have a DHA algal oil complex soft capsule for auxiliary improvement of memory power function, content is prepared from by the component of following parts by weight: DHA algal oil 55 parts, Phosphatidylserine 30 parts, safflower oil 10 parts, astaxanthin oil 5 parts.Described Phosphatidylserine buying is from Dou Shan group of Korea S (DoosanCorporation), described astaxanthin oil adopts purchased from American West Asia promise Imtech (cyanotech), and described safflower oil buying is from Handan morning twilight treasure oils and fats company limited.
Concrete preparation method is as follows:
1) DHA algal oil, Phosphatidylserine, astaxanthin oil, safflower oil are fed intake in cryogenic high pressure homogenizer by component proportioning, in 20 DEG C, under 35MPa condition homogeneous twice, the homogeneous 10min of first time 8000r/min, the homogeneous 5min of second time 10000r/min, obtains algae oil complex emulsion (capsule 's content).
By step 1) gained emulsion press 550mg/ grain specification metering fill soft capsule.Finally carry out low temperature (28 DEG C) low humidity (relative humidity 30%) be dried to dry ball water content 8%, pick ball and obtain the DHA algal oil complex soft capsule preparation that the present invention has auxiliary improvement of memory power.
Auxiliary improvement of memory power effect and safety evaluatio:
Laboratory animal: SPF level female KM kind mice, 3-4 week age, 20 ± 2 grams; 300 mices, are divided into 3 batches at random, often criticize 100 and carry out step down test respectively, keep away dark test, water maze test, often criticize 100 and be divided into 10 groups at random, often organize 10 as negative control group and each tested material various dose test group mice.
Experiment material:
Complete granular Mus feedstuff, sterilized water, Oleum Arachidis hypogaeae semen, tested material comprise: capsule 's content 1 (DHA algal oil 45 parts prepared by the embodiment of the present invention 2, Phosphatidylserine 33 parts, safflower oil 14 parts, astaxanthin oil 8 parts), contrast tested material 2 (DHA algal oil 45 parts, Oleum Arachidis hypogaeae semen 55 parts), contrast tested material 3 (Oleum Arachidis hypogaeae semen 45 parts, Phosphatidylserine 33 parts, safflower oil 14 parts, astaxanthin oil 8 parts)
Experiment given low:
Dose is recommended according to human body, getting 1.7g, 3.3g, 10g capsule 's content respectively and contrasting tested material is dissolved in 100ml Oleum Arachidis hypogaeae semen, and gavage amount is 10ml/kg.bw, obtains low dosage A:0.17g/kg.bw tested material, middle dosage B:0.33g/kg.bw tested material, high dose C:1g/kg.bw tested material
Experimental group:
Negative control group: gavage 10ml/kg.bw Oleum Arachidis hypogaeae semen
Low dosage A1 group: gavage 0.17g/kg.bw capsule 's content 1 of the present invention
Low dosage A2 group: gavage 0.17g/kg.bw contrasts tested material 2
Low dosage A3 group: gavage 0.17g/kg.bw contrasts tested material 3
Middle dosage B1 group: gavage 0.33g/kg.bw capsule 's content 1 of the present invention
Middle dosage B2 group: gavage 0.33g/kg.bw contrasts tested material 2
Middle dosage B3 group: gavage 0.33g/kg.bw contrasts tested material 3
High dose C1 group: gavage 1g/kg.bw capsule 's content 1 of the present invention
High dose C2 group: gavage 1g/kg.bw contrasts tested material 2
High dose C3 group: gavage 1g/kg.bw contrasts tested material 3
Experimental technique:
Every day gavage once, continuous gavage 30 days, after last gavage, next day carries out step down test, keeps away dark test, water maze test.Experimental session is respectively organized mice and is normally ingested, freely drinks water.
Jumping test: mice is put into reaction chamber endoadaptation 3min, passing to 36V alternating current immediately stimulates mice.Then mice is placed on insulated platform, records each mice first time and jump off every Mus in incubation period of platform, 5min and jump off the number of animals (Memory acquisition) that the errors number of platform and each group jump off platform.Retest after 24h or 48h, record first time jumps off the number of animals (memory consolidation) that the errors number of jumping off platform in incubation period of platform, 3min and each group jump off platform.Deconditioning after last gavage, continues raising and within 4 days, carries out test (memory is reproduced) of disappearing.
Step-through test: mice face is put into bright room in hole dorsad, starting timer simultaneously, recording that every Mus enters incubation period (every mice is from putting into bright room to the interval time entering darkroom and shocked by electricity) in darkroom, 5min enters the errors number in darkroom and the number of animals (Memory acquisition) in the financial darkroom of each group.Retest after 24h, records the number of animals (memory consolidation) that every Mus enters errors number and the financial darkroom of each group entering darkroom in incubation period in darkroom, 5min.Deconditioning after last gavage, continues raising and within 4 days, carries out test (memory is reproduced) of disappearing.
Water maze laboratory: the mice training time is defined as 120s, the mice of not reaching home in 120s is all designated as 120s. and trains once every day, continuously training 5 days, and training period continues to give tested material.Finally evaluate with total school grade of 5 days, calculate the number of animals of reaching home in each group of total time of reaching home for 5 days, total errors number and each group 2min.Continue raising after last gavage and within 5 days, carry out test (memory is reproduced) of disappearing.
Result is as shown in table 1, table 2 and table 3;
Table 1: Jumping test result
Note: * P<0.05, * * P<0.01 compared with negative control group
Table 2: step-through test result
Note: * P<0.05, * * P<0.01 compared with negative control group
Table 3: water maze laboratory result
Note: * P<0.05, * * P<0.01 compared with negative control group
Interpretation of result:
Above experimental result display, capsule 's content tested material 30 days prepared by the embodiment of the present invention 2 that continuous per os gavage gives basic, normal, high three various dose of mice, Jumping test, step-through test are all positive result, and wherein tested material of the present invention middle and high dosage experiments group water maze laboratory is also positive findings; Compare mouse memory with negative control group to consolidate, remember reproduction there is significant difference; The middle and high dosage experiments group of contrast tested material also present some positive result in Jumping test, step-through test, water maze laboratory.Show that DHA algal oil complex soft capsule preparation of the present invention has the auxiliary function improving mouse memory; Compare with contrast tested material experimental group, tested material compatibility of the present invention is reasonable, stable, and Improving memory power is remarkably productive; Give each dose of test thing gavage mouse experiment training period in good condition, show that the present invention has auxiliary improvement of memory power DHA algal oil complex soft capsule preparation safety, without bad indication.
Above-mentioned embodiment is the embodiment of the best of the present invention; but embodiment of the present invention are not by the restriction of above-mentioned embodiment; other any without prejudice under the condition of the principle of the invention; the embodiment that can be produced by the form changing parameter, is all contained within protection scope of the present invention.
Claims (7)
1. there is a compositions for auxiliary improvement of memory power function, it is characterized in that, become by following component parts by weights array:
DHA algal oil 40-55 part, Phosphatidylserine 30-40 part, safflower oil 10-20 part, astaxanthin oil 5-8 part.
2. the compositions with auxiliary improvement of memory power function according to claim 1, is characterized in that, is become by following component parts by weights array:
DHA algal oil 45 parts, Phosphatidylserine 33 parts, safflower oil 14 parts, astaxanthin oil 8 parts.
3. the compositions with auxiliary improvement of memory power function according to claim 1, is characterized in that, described DHA algal oil is prepared from by following methods:
1) expect pretreatment: in schizochytrium dry powder, add 2-4 times of water gaging (w/w), concentrated hydrochloric acid regulates PH to 1-2, stirs, dissolves 40-50min, multigelation 2-3 time respectively at 30-40min freezing at-20 DEG C of temperature, 45 DEG C of temperature; Add initial algae dry powder 2 times of water gagings (w/w) again, 6MNaOH regulates PH to 12-13, and ultrasonic power is 120W, working time 20s, and interval time, 15s, worked 40-60 time, carried out ultrasonic vibration broken wall treatment under 4-10 DEG C of condition; And then add algae mud solution equal-volume pure water after ultrasonication process, wash 1-2 time, collect nonaqueous phase pretreatment and to wet algae mud, 50-60 DEG C of heat-wind circulate drying is no more than 10% to water content, obtains pretreatment algae mud siccative;
2) oils and fats extracts: to step 1) add 1-2 times amount 95% ethanol (w/v) in gained pretreatment algae mud siccative, after stirring, be transferred to supercritical CO rapidly
2in extraction column, extraction column ambient temperature controls at 35-45 DEG C, and in extraction column, Stress control is at 35-40MPa, when temperature, pressure are steady, and control CO
2flow velocity 1.6-1.8L/min starts extraction and recovery, and extraction time is 2-3h, collects extraction crude oil;
3) DHA enriching and purifying: to step 2) add 6-8 times amount 0.5MKOH-CH in gained crude oil
3oH solution (w/v), 60 DEG C of heated and stirred are to merging completely, add initial crude oil 2-3 times amount petroleum ether (w/v) again, leave standstill 15-20min after stirring, separated and collected lower floor filtrate, concentrated hydrochloric acid regulates PH to 1-2, add initial crude oil 6-8 times amount petroleum ether (w/v) afterwards, leave standstill 25-30min, collection ether layer solution decompression is concentrated into removes petroleum ether completely, obtains free oil; Then add rapidly free oil 10-15 times amount 12% carbamide alcoholic solution (w/v), after stirring under 0 DEG C of condition winterization 16-20h; Winterization terminates rear rapid sucking filtration, collects the filtrate being enriched unsaturated fatty acid DHA; Initial free oil 2-4 times amount pure water (w/v) is added in filtrate, concentrated hydrochloric acid regulates PH to neutral, add initial free oil 2-4 times amount petroleum ether (w/v), leave standstill and collect ether layer, with pure water washing 1-2 time, collect ether layer, add the anhydrous sodium sulfate (v/w) of ether layer liquor capacity 2-3%, carry out distilling under reduced pressure after the 2-4h that dewaters and collect enrichment DHA algal oil;
4) DHA algal oil finished product is refined: to step 3) add 0.02% vitamin E (w/v) and 0.015% sodium L-ascorbate-2-phosphate (w/v) in gained enrichment DHA algal oil, winterization 8-12h at 4 DEG C of temperature, obtains light yellow refining DHA algal oil.
4. a preparation method with the compositions of auxiliary improvement of memory power function according to claim 1 and 2, it is characterized in that, concrete steps are as follows:
DHA algal oil, Phosphatidylserine, astaxanthin oil, safflower oil are fed intake in cryogenic high pressure homogenizer by component proportioning, in 15-20 DEG C, homogeneous under 30-35MPa condition.
5. a preparation method with the compositions of auxiliary improvement of memory power function according to claim 4, is characterized in that, homogeneous is twice homogenization, the homogeneous 5-8min of the homogeneous 10-15min of first time 6000-8000r/min, second time 8000-10000r/min.
6. one kind contains the capsule with the compositions of auxiliary improvement of memory power function described in claim 1 or 2; It is characterized in that: described compositions is as the content of capsule.
7. the preparation method of a capsule according to claim 6; It is characterized in that: concrete step is:
Contents Fill is entered soft capsule, finally under low temperature (20-30 DEG C), low humidity (relative humidity 22%-28%) condition, is dried to water content at 6%-8%.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106235309A (en) * | 2016-07-29 | 2016-12-21 | 李显堂 | A kind of Functional Food Formulations of brain-strengthening eye protection and preparation method thereof |
| CN106942727A (en) * | 2017-04-20 | 2017-07-14 | 北京市永康年健康科技有限责任公司 | It is a kind of to be used to reduce DHA algal oil product of blood fat and its preparation method and application |
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| CN107279992A (en) * | 2017-06-02 | 2017-10-24 | 杭州鑫伟低碳技术研发有限公司 | It is a kind of to be used to protect astaxanthin product of eyesight improving memory and preparation method thereof |
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| CN109288005A (en) * | 2018-10-31 | 2019-02-01 | 北京斯利安药业有限公司 | A kind of composition with auxiliary improvement of memory power function, its application and preparation method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120051458A (en) * | 2010-11-12 | 2012-05-22 | 주식회사 두산 | Method of preparing a composition including astaxanthin and dha- and/or epa-conjugated phosphatidylserine using krill-derived lecithin, and a composition prepared by the method |
| CN102461677A (en) * | 2010-11-19 | 2012-05-23 | 嘉里特种油脂（上海）有限公司 | Composite improving stability of long-chained polyunsaturated fatty acid and application thereof |
| CN103609752A (en) * | 2013-12-02 | 2014-03-05 | 内蒙古伊利实业集团股份有限公司 | Composition for improving cognitive function of brain, and preparation method and application thereof |
-
2015
- 2015-12-30 CN CN201511027817.9A patent/CN105497093B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120051458A (en) * | 2010-11-12 | 2012-05-22 | 주식회사 두산 | Method of preparing a composition including astaxanthin and dha- and/or epa-conjugated phosphatidylserine using krill-derived lecithin, and a composition prepared by the method |
| CN102461677A (en) * | 2010-11-19 | 2012-05-23 | 嘉里特种油脂（上海）有限公司 | Composite improving stability of long-chained polyunsaturated fatty acid and application thereof |
| CN103609752A (en) * | 2013-12-02 | 2014-03-05 | 内蒙古伊利实业集团股份有限公司 | Composition for improving cognitive function of brain, and preparation method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 秦昌蓉: "富油微藻中油脂的提取及DHA富集纯化的研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
| 颜素珠: "《新经济植物学》", 31 July 1996, 暨南大学出版社 * |
| 高俊全，等: "《虾青素：健康新世纪的奥秘》", 31 July 2013, 中国医药科技出版社 * |
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