CN105496872A - Sun-blocking composition and daily chemical product with sun-blocking effect - Google Patents

Sun-blocking composition and daily chemical product with sun-blocking effect Download PDF

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Publication number
CN105496872A
CN105496872A CN201510988168.2A CN201510988168A CN105496872A CN 105496872 A CN105496872 A CN 105496872A CN 201510988168 A CN201510988168 A CN 201510988168A CN 105496872 A CN105496872 A CN 105496872A
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sun
extract
sunscreen
oil
sunscreen composition
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CN105496872B (en
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符志冲
谌宏学
肖瑞光
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Guangzhou Shancaoji Cosmetic Co Ltd
ZHAOQING DICAI DAILY CHEMICAL SCIENCE AND TECHNOLOGY Co Ltd
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Guangzhou Shancaoji Cosmetic Co Ltd
ZHAOQING DICAI DAILY CHEMICAL SCIENCE AND TECHNOLOGY Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Dermatology (AREA)
  • Cosmetics (AREA)

Abstract

The invention relates to sun-blocking composition and a daily chemical product with a sun-blocking effect. The sun-blocking composition comprises raw materials in percentage by mass as follows: 0.01%-3% of a purple coneflower extract, 0.01%-3% of an emblic extract, 0.01%-3% of a meadowrue root and rhizome extract, 0.01%-10% of crambe abyssinica seed oil, 0-20% of a physical sunscreen and the balance of an auxiliary. The sun-blocking composition contains various plant ingredients and has multiple effects including ultraviolet light absorption, inflammation resistance, after-sunburn repair and the like; the sun-blocking composition contains naturally sourced ingredients, is safe and mild and has broader market prospect.

Description

A kind of sunscreen composition and there is the cosmetics of everyday use of sun-proof function
Technical field
The invention belongs to cosmetic field, be specifically related to a kind of sunscreen composition and there is the cosmetics of everyday use of sun-proof function.
Background technology
Appropriate ultraviolet (UV) irradiates the antibacterial that skin can kill skin surface, and accelerates the generation of vitamin D, thus promotes the absorption of calcium, is conducive to the growth promoter of human body.But, excessive ultraviolet irradiates for a long time and can produce adverse influence to skin, reason is the 26S Proteasome Structure and Function that ultraviolet can change individual cells component (as protein, lipid and DNA molecular), infringement human immune system, accelerate skin aging, produce various dermatosis, time even serious, can skin carcinoma be brought out.Sun-proofly people have been caused to pay close attention to widely, raising along with people's living standard, the demand to fair skin and the understanding to the harm of excessive exposure ultraviolet are strengthened gradually, people start to use sun-proof class cosmetics to prevent ultraviolet to the injury of human body in a large number, and the research and development of sun-proof class cosmetics become one of focus of cosmetics research and development.It is single that sun-proof class cosmetics in the market also exist sun-proof approach, and the skin care item that SPF (sun protection factor) is higher contain a large amount of physical sunscreen agent and chemical sun mostly, particularly chemical sun, very large pressure can be caused to skin after using, cause skin allergy and the sense of discomfort in use and insecurity can be caused.Along with the cry of people's back to nature grows to even greater heights, application natural plant composition has caused cosmetics worker to study widely as sun care preparations raw material.In natural plants, some composition has sun-proof function, they to skin action temperature and, zest is little, and safety is high, also have absorb and shielding ultraviolet rays, opposing inflammation and reparation after shining effect.Therefore, the application of natural plant composition in sun care preparations has very wide prospect.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of sunscreen composition, described sunscreen composition contains various plants composition, and it has multi-efficiencies such as absorbing ultraviolet, opposing inflammation and the rear reparation of solarization; Sunscreen composition ingredient origin provided by the invention is natural, and safety is gentle, has more wide market prospect.
For achieving the above object, the present invention adopts following technical scheme:
A kind of sunscreen composition, described sunscreen composition is made up of following raw material by mass percentage:
Echinacea angustifolia extract 0.01 ~ 3%;
Fructus Phyllanthi extract 0.01 ~ 3%;
Radix et Rhizoma Thalictri extract 0.01 ~ 3%;
Shepherd's purse seed oil 0.01 ~ 10% is saved at deep-sea two;
Physical sunscreen agent 0 ~ 20%;
Surplus is auxiliary agent.
Deep-sea two is saved shepherd's purse seed oil and is formed by the seed cold pressing of two joint shepherd's purses of Mediterranean.Shepherd's purse seed oil is saved at deep-sea two, and oxidation stability is good, and skinfeel silk is sliding, joyful, and remarkable toner dispersibility, is rich in skin nutrition composition: OMEGA-3,6, and 9 and docosenoic acid (C22:1).Obvious absorption peaks is had, simultaneously its effect that physical sunscreen agent is had good dispersion effect and repaired after shining in the wave band interval of UVA.
Echinacea purpurea Moench (Echinacea) originates from North America and a Mexican kind of plant; Its extract is by strengthening the life-span of Skin Cell to the toleration prolongation cell of pressure, and can effectively suppress to irradiate by UVB the inflammation caused, it has obvious absorption peaks in UVB and UVA wave band district simultaneously.
Fructus Phyllanthi orders again Phyllanthus emblica L., Niuganguo, because its taste " bitter first ,sweet later " gains the name.There is various trace elements and the mineral such as abundant injection Vitamin B_6, B, simultaneously also containing 14 seed amino acids of needed by human body and superoxide dismutase (SOD) and the organic selenium that enriches, especially vitamin C, containing vitamin C 500 ~ 1841 milligrams in every 100 grams of fresh Phyllanthus emblica L.s, higher than the Fructus actinidiae chinensis of the king have vitamin 4 times, higher than mandarin orange 25 times, there is plurality kinds of health care medical function.Usual purposes is food, beverage and medical aspect, but the present invention is mainly applied in the sun-proof field of skin care item, and because it has obvious absorption peaks in the wave band interval of UVA, simultaneously abundant vitamin is conducive to the reparation of shining rear skin.
Radix et Rhizoma Thalictri extract source is the leafy Thalictrum aquilegifolium L. var. sibiricum Regel of Ranunculaceae thalictrum spp and plateau Thalictrum aquilegifolium L. var. sibiricum Regel, is used as medicine with root.Root digs out by spring, autumn, cuts off stem and leaf on the ground, washes away earth, dry.Leafy Thalictrum aquilegifolium L. var. sibiricum Regel: perennial herb, plant is all without hair.Underground have horizontal walk root stock, the root that raw many bars are elongated, all yellow.Stem is high 90 ~ 150 centimetres, and long branch is arranged at top.Stem middle leaf is go out for three times three or nearly winglike compound leaf; Blade reaches 28 centimetres, lobule herbaceous stem, avette, width egg shape or subcircular, long 1 ~ 2.5 centimetre, wide 0.5 ~ 1.5 centimetre, and not obvious 3 shallowly split, and have thin knuckle-tooth, arteries and veins is several not to swell.9 ~ October blooms, and inflorescence is coniform, multi-branched; Bennet is thin, long 0.7 ~ 1.5 centimetre; Flower diameter about 7 millimeters; Sepal 4, petal-shaped, white or be with faint yellow, caducous, oval, long 3 ~ 4.5 millimeters; Without petal; Stamen is most, and long 6 ~ 7 millimeters, there is short point on flower pesticide top, and filigree is thread; Carpel 4 ~ 6, stigma bar shaped, has narrow wing.Achene spindle, long 3 millimeters.Its Main Function is heat clearing and damp drying, eliminating fire and detoxication.The present invention is applied in sun-proof field, and it has obvious absorption peaks in the wave band interval of UVA and UVB.
Shepherd's purse seed oil four kinds of natural extracts and physical sunscreen agent, quintessence oil and other auxiliary agent are saved with the use of obtaining a kind of sunscreen composition in Echinacea angustifolia extract, Fructus Phyllanthi extract, Radix et Rhizoma Thalictri extract, deep-sea two by the present invention, the interphase interaction of the various active composition in this sunscreen composition, it has multi-efficiencies such as absorbing ultraviolet, Synergistic spf value, opposing inflammation and the rear reparation of solarization; And sunscreen composition provided by the invention contains natural plant composition, safety is gentle, has toxic and side effects little, the feature that clinical efficacy is good.
Preferably,
Preferably, described sunscreen composition is made up of following raw material by mass percentage:
Echinacea angustifolia extract 0.1 ~ 1%;
Fructus Phyllanthi extract 0.1 ~ 1%;
Radix et Rhizoma Thalictri extract 0.1 ~ 1%;
Shepherd's purse seed oil 0.3 ~ 5% is saved at deep-sea two;
Physical sunscreen agent 5 ~ 20%;
Surplus is auxiliary agent.
Preferably, described auxiliary agent is one or more in deionized water, grease, surfactant, thickening agent, chelating agen, solubilizing agent, quintessence oil or antiseptic.
More preferably, described sunscreen composition is made up of following raw material by mass percentage:
Echinacea angustifolia extract 0.6%;
Shepherd's purse oil 4% is saved at deep-sea two;
Fructus Phyllanthi extract 0.6%;
Radix et Rhizoma Thalictri extract 0.6%;
Physical sunscreen agent 8%;
Quintessence oil 0.3%;
Grease 10%;
Thickening agent 4%;
Surfactant 3%;
Antiseptic 0.6%;
Surplus is water.
In the present invention, select to have the quintessence oil eliminating free radical or opposing inflammatory effect, preferably, described quintessence oil is one or more in Flos Rosae Rugosae quintessence oil, chamomile essential oil, sandalwood essential oil, Peppermint essential oil, Jojoba oil, tea tree ethereal oil or rosemary ethereal oil.
In the present invention, described physical sunscreen agent can be the conventional various physical sunscreen agent in this area, and preferably, described physical sunscreen agent is one or both in titanium dioxide or zinc oxide.
Preferably, described Echinacea angustifolia extract, Fructus Phyllanthi extract or Radix et Rhizoma Thalictri extract adopt microwave extracting.
The mechanism of microwave extracting can from the viewpoint of two, and microwave radiation process is that frequency electromagnetic waves penetrates spe medium on the one hand, arrives internal batch vascular bundle and glandular cell's system.Owing to absorbing microwave energy, cell interior temperature rises rapidly, makes its cell interior pressure exceed cell wall expansion ability to bear, cell rupture.In cell, effective ingredient freely flows out, and under lower temperature conditions, spe medium is caught and dissolved.By filtering further and being separated, just obtain extraction material.On the other hand, the electromagnetic field that microwave produces accelerates to be extracted fractions to extractant interface diffusion rate, during with moisture extractant, under microwave field, hydrone high-speed rotation is called excited state, this is a kind of high-energy labile state, or hydrone vaporization, strengthens the power of extraction component; Or hydrone itself releases energy and gets back to ground state, the energy delivery discharged gives other material molecules, accelerate its warm-up movement, the molecule shortening extracted component is diffused into the time at extractant interface by internal batch, thus make extraction rate improve several times, also reduce extraction temperature simultaneously, ensure the quality extracted to greatest extent.
Preferably, the extractant that Echinacea angustifolia extract is used is glycerine water solution; Fructus Phyllanthi extract extractant used is butanediol aqueous solution; Radix et Rhizoma Thalictri extract extractant used is aqueous solution of propylene glycol.Preferably, in the process of Echinacea angustifolia extract microwave extracting, microwave frequency is 400 ~ 250000MHz; Further preferably, microwave frequency is 2450MHz.
Preferably, in the process of Fructus Phyllanthi extract microwave extracting, microwave frequency is 300 ~ 300000MHz; Further preferably, microwave frequency is 2450MHz.
Preferably, in the process of Radix et Rhizoma Thalictri extract microwave extracting, microwave frequency is 600 ~ 300000MHz; Further preferably, microwave frequency is 2450MHz.
For extending the fragrance remaining time of above-mentioned quintessence oil, the present invention adopts the above-mentioned quintessence oil of microcapsule technology embedding treatment.
Preferably, the wall material that microcapsule technology embedding treatment is used is one or more in arabic gum, soybean protein, maltodextrin, soluble starch, lecithin or cyclodextrin; When the micro encapsulation process carrying out quintessence oil, the mass ratio of wall material and quintessence oil is preferably 3 ~ 7:1, and micro encapsulation temperature is 35 ~ 60 DEG C, and the micro encapsulation time is 0.01 ~ 2 hour.
There is a cosmetics of everyday use for sun-proof function, described cosmetics of everyday use be above-mentioned sunscreen composition prepare sun protection gel, sunscreen, sunscreen cream, sunscreen spray, sun water, sun-proof ballpoint, sunscreen stick, BB frost, sun screen, isolation breast, sun-proof sizing, shampoo or hair conditioner.
Compared with prior art, the present invention has following beneficial effect:
The present invention, by various plants active component mixture, obtains a kind of sunscreen composition; This sunscreen composition is due to the interaction of wherein various active composition, have and absorb ultraviolet, Synergistic spf value, diminish inflammation and shine effect of rear reparation, because sunscreen composition of the present invention contains natural plant composition, it is little that it has toxic and side effects, the feature that clinical efficacy is good.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further explained, but embodiments of the present invention is not limited in any way.Unless stated otherwise, involved in embodiment reagent, method are the conventional reagent in this area and method.
In embodiment 1 ~ 9, Echinacea angustifolia extract used adopts microwave-assisted extraction technique, chooses Echinacea purpurea Moench dry rhizome, take mass fraction as the glycerol liquor of 50% is solvent, and the microwave frequency of extraction is 2450MHz.Other extraction process is carried out with reference to prior art, concentrated, obtains Echinacea angustifolia extract; Fructus Phyllanthi extract used adopts microwave-assisted extraction technique, chooses the dry fruit of Fructus Phyllanthi, take mass fraction as the butanediol of 40% is solvent, and the microwave frequency of extraction is 2450MHz.Other extraction process is carried out with reference to prior art, concentrated, obtains Fructus Phyllanthi extract; Radix et Rhizoma Thalictri extract used adopts microwave-assisted extraction technique, chooses the dry rhizome of Radix et Rhizoma Thalictri, with mass fraction be the propylene glycol water of 60% for solvent, the microwave frequency of extraction is 2450MHz.Other extraction process is carried out with reference to prior art, concentrated, obtains Radix et Rhizoma Thalictri extract.
In embodiment 10, Echinacea angustifolia extract used adopts microwave-assisted extraction technique, and choosing the dry rhizome of Echinacea purpurea Moench, take mass fraction as the glycerol liquor of 50% is solvent, and the microwave frequency of extraction is 400MHz.Other extraction process is carried out with reference to prior art, concentrated, obtains Echinacea angustifolia extract; Fructus Phyllanthi extract used adopts microwave-assisted extraction technique, chooses the dry fruit of Fructus Phyllanthi, take mass fraction as the butanediol of 40% is solvent, and the microwave frequency of extraction is 300MHz.Other extraction process is carried out with reference to prior art, concentrated, obtains Fructus Phyllanthi extract; Radix et Rhizoma Thalictri extract used adopts microwave-assisted extraction technique, chooses dry Radix et Rhizoma Thalictri rhizome, with mass fraction be the propylene glycol water of 60% for solvent, the microwave frequency of extraction is 600MHz.Other extraction process is carried out with reference to prior art, concentrated, obtains Radix et Rhizoma Thalictri extract.
In embodiment 11, Echinacea angustifolia extract used adopts microwave-assisted extraction technique, chooses the dry rhizome of Echinacea purpurea Moench, take mass fraction as the glycerol liquor of 50% is solvent, and the microwave frequency of extraction is 250000MHz.Other extraction process is carried out with reference to prior art, concentrated, obtains Echinacea angustifolia extract; Fructus Phyllanthi extract used adopts microwave-assisted extraction technique, chooses the dry fruit of Fructus Phyllanthi, take mass fraction as the butanediol of 40% is solvent, and the microwave frequency of extraction is 300000MHz; Radix et Rhizoma Thalictri extract used adopts microwave-assisted extraction technique, chooses dry Radix et Rhizoma Thalictri rhizome, with mass fraction be the propylene glycol water of 60% for solvent, the microwave frequency of extraction is 300000MHz.Other extraction process is carried out with reference to prior art, concentrated, obtains Radix et Rhizoma Thalictri extract.
For embodying the impact of performance of sunscreen composition of the present invention, the present invention is also provided with two reference examples, do not add Echinacea angustifolia extract, Fructus Phyllanthi extract, Radix et Rhizoma Thalictri extract and deep-sea two in reference examples 1 and save shepherd's purse seed oil four kinds of natural plant components, other component is identical with embodiment 1; Do not add Echinacea angustifolia extract, Fructus Phyllanthi extract, Radix et Rhizoma Thalictri extract and deep-sea two in reference examples 2 and save shepherd's purse seed oil four kinds of natural plant compositions yet, but add 5 parts of chemical sun, other component is identical with embodiment 1, and in reference examples 2, described chemical sun is OXYBENZONE.
In embodiment 1 ~ 11 and reference examples 1 ~ 2, select the Flos Rosae Rugosae quintessence oil of the coated process of beta-schardinger dextrin-, the weight ratio of beta-schardinger dextrin-and Flos Rosae Rugosae quintessence oil is 5:1, and micro encapsulation temperature is 40 DEG C, and the micro encapsulation time is 1.5 hours.
The sunscreen composition of embodiment 1 ~ 11 and reference examples 1 ~ 2 is prepared into cream type, tests its properties; The concrete formula of the sunscreen composition of embodiment 1 ~ 11 and reference examples 1 ~ 2 sees the following form 1.
The sunscreen composition composition formula of table 1 embodiment 1 ~ 11 and reference examples 1 ~ 2
The cream type sunscreen product that the sunscreen composition of embodiment 1 ~ 11 and reference examples 1 ~ 2 is prepared carry out SPF, UVAPF, patch test, average COX-2 suppression ratio sun-proof performance test and carry out result of use evaluation, method of testing is as follows, and test result sees the following form 2 and table 3.
(1) SPF performance test SPF performance test uses ex vivo technique to measure according to following scheme:
At first, the weight of the PMMA substrate (purchased from SCHONBERGGmbH & Co.KG, Hamburg/Germany) that measured surface is coarse.Then to be tested batch to be deposited on substrate and to use 7 microns of lower pull-rods (drawdownbar) floating to obtain thin conforming layer fast subsequently.Make this layer of drying about 20 minutes, and measure the weight of substrate+uniform drying layer.The UV of uniform drying layer absorbs and uses the multiple measurements of LABSPHERE2000S analyser on layer.The solid matter percentage ratio of this layer uses solid matter analyser to measure.Utilize the weight of dry film and the solid matter content of layer, the weight of the rear instant original wet layer of deposition can be calculated and therefore calculate its density.Use this information, can following formulae discovery SPF be passed through:
The wherein spectral irradiance of E (X)=standard solar spectrum; The erythemal action spectrum (erythemalactionspectrum) of S (λ)=af at wavelength lambda and the spectral absorption (spectralabsorbance) of A (λ)=af at wavelength lambda (calculate correction factor with extrapolation for determining that (sample test is counted (9 points, average) under the wet layer density of 2.0mg/cm2; Blankscan number (8 times); Solar irradiance (290nm-400nm).
UV-2000S is a stable top testing equipment, can detect the diffuse transmittance of outturn sample to light in 280nm-410nm wave-length coverage fast.The double beam spectrometer with general diffused lighting function accurately can be tested high scattering sample, and without the need to using light filter to prevent the overexposure of sample.Test data conversion can be SPF (sun protection factor) (SPF) by its application software automatically.
(2) test of UVAPF performance test UVA-PF adopts COLIPA method
Use UV-2000S analyser to measure, sample test is counted (9 points, average); Blankscan number (8 times); Solar irradiance (290nm-400nm)
Principle steps is as follows: the mensuration of UVAPF is undertaken by following steps:
Step 1: by spreading upon the in-vitro measurements of the absorbance A 0 (λ) on PMMA panel products before any UV irradiates;
Step 2: coefficient of utilization ' C ' carries out the mathematics correction of initial UV spectrum to obtain the external SPF(0%UV dosage equaling nominal (in vivo) SPF).Use A0 (λ) and C to calculate initial UVA subsequently and protect (UVAPF0);
Step 3: calculate single UV dosage D, proportional with UVAPF0 (D=UVAPF0 × 1.2);
Step 4: under making described sample be exposed to the UV calculating UV dosage D;
The external transmission measurement of the sunscreen product after step 5:UV irradiates, with A (λ) data acquisition the 2nd UV spectrum;
Step 6: the number carrying out the second spectrum (after UV irradiation) according to the identical C coefficient previously measured in step 2;
Learn and revise, calculate external UVA protecting factor UVAPF subsequently.Computing formula is as follows:
Test data can be UVAPF value by COLIPA method migration by UV-2000S analyser its application software automatically.
(3) patch test
Patch test method is, by the routine subject of subject enrollment Standard Selection 30, select qualified patch material, sample is put into speckle examination device (amount of samples is 0.020-0.025g), simultaneously one of them speckle examination device is that blank is declined any material and is blank.All be affixed on the bent side of forearm of experimenter by what have a sample with the patch device of blank, gently press with palm and make it to stick equably on skin, continue 24h.Interval 30min after removal patch device, observes dermoreaction after impression disappears.
The criterion of patch test is, judge according to " diagnostic criteria of cosmetics contact dermatitis and treatment principle " classification: recipient site is reactionless as (0), skin occurs that light red speckle is for (1), skin occurs that erythema, infiltration or pimple are for (2), skin occurs that edematous erythema, pimple are for (3), and skin occurs that significantly redness, companion's pimple or bulla are (4);
(4) average COX-2 suppression ratio test
Average COX-2 suppression ratio refers to the activity by suppressing COX-2 enzyme, alleviate or slow down UVB line induce the inflammation of generation.Its numerical value is higher represents that opposing is better by the tan severely effect that causes inflammation of ultraviolet.
Test method is, weigh 0.5 gram of test products, by the cyclooxygenase (COX)-2 of equivalent and fluorogenic substrate 10-acetyl group-3, 7-dihydroxyphenazine (ADHP) mixes mutually with haemachrome, reaction is started by adding equivalent substrate arachidonic acid, arachidonic acid can be converted into PGG2 (PGG2) by COX-2, PGG2 (PGG2) is converted into the PGH2 (PGH2) of equal number then, in this course of reaction, ADHP is converted into fluorescence resorufin (fluorescent end product), measure fluorescence intensity (λ Ex535nm, λ Em590nm).That fluorescence intensity obtained with the testing time and most that fluorescence intensity of initial time compare, and the reduction obtained is the suppression ratio of COX-2 enzyme.
(5) result of use evaluation
Result of use evaluation mainly refers to by 20 aspiration triers through professional training, the sample of 80ul is applied to the back of the hand, mildly circle round with another hands and smear 8 times, then with the mode of marking evaluate successively the texture of sample, spreadability, flexibility, absorbability, with rear joyful sense; Evaluation criterion: very good (8-10), good (6-8), general (4-6), poor (2-4), poor (0-2), the scoring best result of properties is 10 points, is minimumly divided into 0 point, mark is higher, and the performance representing this product is better, and total score is 50 points, numerical value is higher, and effect is better.
To the properties test result of the cream type sunscreen product that the sunscreen composition of embodiment 1 ~ 11 and reference examples 1 ~ 2 prepares as table 2 and table 3:
The sun-proof performance test of the product of table 2 embodiment 1 ~ 11 and reference examples 1 ~ 2
Table 3: the result of use evaluation of the product of embodiment 1 ~ 11 and reference examples 1 ~ 2
As can be seen from upper table 2 and table 3, the sun-proof result of the product of the cream type that sunscreen composition provided by the invention prepares is better, and result of use is had higher rating, be significantly better than not adding the reference examples 1 of any natural constituents and with the addition of the reference examples 2 of chemical sun; Sunscreen composition provided by the invention has and absorbs ultraviolet, Synergistic spf value, diminishes inflammation and shine effect of rear reparation, and contains natural plant composition, has toxic and side effects little, the feature that clinical efficacy is good.
Above-described embodiment is the present invention's preferably embodiment, but embodiments of the present invention are not restricted to the described embodiments, above-mentioned sunscreen composition provided by the invention can make the cosmetics of everyday use of the various dosage forms such as sun protection gel, sunscreen, sunscreen cream, sunscreen spray, sun water, sun-proof ballpoint, sunscreen stick, BB frost, sun screen, isolation breast, sun-proof sizing, shampoo, hair conditioner.
The change done under other any does not deviate from spirit of the present invention and principle, modification, substitute, combine, simplify the substitute mode that all should be equivalence, be included within protection scope of the present invention.

Claims (9)

1. a sunscreen composition, is characterized in that, described sunscreen composition is made up of following raw material by mass percentage:
Echinacea angustifolia extract 0.01 ~ 3%;
Fructus Phyllanthi extract 0.01 ~ 3%;
Radix et Rhizoma Thalictri extract 0.01 ~ 3%;
Shepherd's purse seed oil 0.01 ~ 10% is saved at deep-sea two;
Physical sunscreen agent 0 ~ 20%;
Surplus is auxiliary agent.
2. sunscreen composition according to claim 1, it is characterized in that, described sunscreen composition is made up of following raw material by mass percentage:
Echinacea angustifolia extract 0.1 ~ 1%;
Fructus Phyllanthi extract 0.1 ~ 1%;
Radix et Rhizoma Thalictri extract 0.1 ~ 1%;
Shepherd's purse seed oil 0.3 ~ 5% is saved at deep-sea two;
Physical sunscreen agent 5 ~ 20%;
Surplus is auxiliary agent.
3. sunscreen composition according to claim 1 or 2, is characterized in that, described auxiliary agent is one or more in deionized water, grease, surfactant, thickening agent, chelating agen, solubilizing agent, quintessence oil or antiseptic.
4. sunscreen composition according to claim 2, it is characterized in that, described sunscreen composition is made up of following raw material by mass percentage:
Echinacea angustifolia extract 0.6%;
Shepherd's purse oil 4% is saved at deep-sea two;
Fructus Phyllanthi extract 0.6%;
Radix et Rhizoma Thalictri extract 0.6%;
Physical sunscreen agent 8%;
Quintessence oil 0.3%;
Grease 10%;
Thickening agent 4%;
Surfactant 3%;
Antiseptic 0.6%;
Surplus is water.
5. sunscreen composition according to claim 3, it is characterized in that, described quintessence oil is one or more in Flos Rosae Rugosae quintessence oil, chamomile essential oil, sandalwood essential oil, Peppermint essential oil, Jojoba oil, tea tree ethereal oil or rosemary ethereal oil.
6. sunscreen composition according to claim 1, is characterized in that, described physical sunscreen agent is one or both in titanium dioxide or zinc oxide.
7. sunscreen composition according to claim 1, is characterized in that, described Echinacea angustifolia extract, Fructus Phyllanthi extract or Radix et Rhizoma Thalictri extract adopt microwave extracting.
8. sunscreen composition according to claim 1, it is characterized in that, described quintessence oil is the quintessence oil after microcapsule technology embedding treatment.
9. one kind has the cosmetics of everyday use of sun-proof function, it is characterized in that, described cosmetics of everyday use for prepared by sunscreen composition described in the arbitrary claim of claim 1 ~ 8 sun protection gel, sunscreen, sunscreen cream, sunscreen spray, sun water, sun-proof ballpoint, sunscreen stick, BB frost, sun screen, isolation breast, sun-proof sizing, shampoo or hair conditioner.
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