CN105487218B - Micro- scanner and micro- scan method - Google Patents

Micro- scanner and micro- scan method Download PDF

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Publication number
CN105487218B
CN105487218B CN201511007566.8A CN201511007566A CN105487218B CN 105487218 B CN105487218 B CN 105487218B CN 201511007566 A CN201511007566 A CN 201511007566A CN 105487218 B CN105487218 B CN 105487218B
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microscope group
micro
light
reflective
slice
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CN105487218A (en
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胡木荣
沈晓江
杨鹏
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Ningbo Sunny Instruments Co Ltd
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Ningbo Sunny Instruments Co Ltd
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    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/36Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361Optical details, e.g. image relay to the camera or image sensor
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/18Arrangements with more than one light path, e.g. for comparing two specimens

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

The present invention provides a kind of micro- scanner and micro- scan method, wherein micro- scanner includes: scanner ontology, eyepiece device and the switching device for realizing optical path switching;Wherein, by the light supply apparatus in scanner ontology, the platform for placing slice, objective lens, the reflective microscope group of third, the second auxiliary microscope group, the first reflective microscope group, the first auxiliary microscope group, the second reflective microscope group, steering prism and eyepiece in switching device and eyepiece device form the first optical path;Second optical path is formed by the light supply apparatus in scanner ontology, the platform for placing slice, objective lens, the reflective microscope group of third, the second auxiliary microscope group, switching device and micro- camera, so as to pass through switching device, so that micro- scanner both can be used for carrying out slice scanning, it can be used for carrying out microexamination again, it avoids realizing above-mentioned two function using the micro- scanner of boxlike and conventional microscope, the working efficiency for improving micro- scanner, reduces costs.

Description

Micro- scanner and micro- scan method
Technical field
The present invention relates to light technical field more particularly to a kind of micro- scanner and micro- scan methods.
Background technique
Currently, the existing micro- scanner of boxlike amplifies scanning simultaneously to slice using micro- camera, attachment lens etc. Slice after exporting magnified sweep, so that user checks.However, in the prior art, the micro- scanner of existing boxlike and without There is eyepiece etc., when user needs to observe slice, user also needs to observe using traditional microscope, to drop The low working efficiency of the micro- scanner of boxlike, improves cost.
Summary of the invention
The present invention provides a kind of micro- scanner and micro- scan method, for solving the micro- scanning of boxlike in the prior art The problem of instrument working efficiency difference.
The first aspect of the invention is to provide a kind of micro- scanner, comprising: scanner ontology, eyepiece device and is used for Realize the switching device of optical path switching;Wherein,
The eyepiece device includes: the first reflective microscope group, the first auxiliary microscope group, the second reflective microscope group, turns to prism and mesh Mirror;
The scanner ontology includes: light supply apparatus, the platform for placing slice, objective lens, the reflective microscope group of third, the Two auxiliary microscope groups and micro- camera;
Wherein, by the light supply apparatus, the platform for placing slice, objective lens, the reflective microscope group of third, the second auxiliary mirror Group, the first reflective microscope group, the first auxiliary microscope group, the second reflective microscope group, turns to prism and eyepiece the first light of formation at switching device Road;
By the light supply apparatus, the platform for placing slice, objective lens, the reflective microscope group of third, the second auxiliary microscope group, cut Changing device and micro- camera form the second optical path.
Further, the switching device includes: the 4th reflective microscope group and lens switch controller;
The lens switch controller is connect with the 4th reflective microscope group, for controlling the anti-of the 4th reflective microscope group The light of the second auxiliary microscope group is reflexed to the described first reflective microscope group and reaches eyepiece by firing angle degree, or by described the The light of two auxiliary microscope groups is transferred to the micro- camera.
Further, the scanner ontology further include: the first attachment lens group;
Wherein, by light supply apparatus, for place slice platform, objective lens, the reflective microscope group of third, second auxiliary microscope group, Switching device, the first attachment lens group and micro- camera form the second optical path.
Further, the micro- scanner further include: fluorescent apparatus;
The fluorescent apparatus is arranged on the optical circuit path between the reflective microscope group of the third and the second auxiliary microscope group.
Further, the micro- scanner further include: light source switch controller;
The light source switch controller is connect with the fluorescent apparatus and the light supply apparatus respectively, described glimmering for controlling Electro-optical device and the light supply apparatus open or close.
Further, the lower section of the platform is arranged in the light supply apparatus;
The light emission direction of the light supply apparatus is towards the platform.
Further, the light emission direction of the fluorescent apparatus is towards the reflective microscope group of the third, so that the third is reflective The light of the fluorescent apparatus is reflexed to the objective lens by microscope group, and the light is sent to described put down by the objective lens Slice on platform.
Further, the micro- scanner further include: master controller, the master controller and lens switching are controlled Device connection processed, for sending switching command to the lens switch controller, so that the lens switch controller is according to switching Instruction controls the reflection angle of the 4th reflective microscope group.
In the present invention, by providing a kind of micro- scanner, comprising: scanner ontology, eyepiece device and for realizing light The switching device of road switching;Wherein, eyepiece device includes: the first reflective microscope group, the first auxiliary microscope group, the second reflective microscope group, turns To prism and eyepiece;Scanner ontology include: light supply apparatus, for place slice platform, objective lens, the reflective microscope group of third, Second auxiliary microscope group and micro- camera;Wherein, by light supply apparatus, the platform for placing slice, objective lens, third reflective mirror Group, switching device, the first reflective microscope group, the first auxiliary microscope group, the second reflective microscope group, turns to prism and mesh at the second auxiliary microscope group Mirror forms the first optical path;By light supply apparatus, the platform for placing slice, objective lens, the reflective microscope group of third, the second auxiliary mirror Group, switching device and micro- camera form the second optical path, so as to pass through switching device, so that micro- scanner can both be used In carrying out slice scanning, and it can be used for carrying out microexamination, so that micro- scanner can integrate slice scanning and micro- sight Two functions are examined, avoids realizing above-mentioned two function using the micro- scanner of boxlike and conventional microscope, improves micro- sweep The working efficiency for retouching instrument, reduces costs.
The second aspect of the invention also provides a kind of micro- scan method, comprising:
Light supply apparatus is that the slice being placed on platform is illuminated, and light is reflected by objective lens through the reflective microscope group of third To the second auxiliary microscope group;
Light is transferred to micro- camera by switching device by the second auxiliary microscope group, so that micro- camera is carried out by light Slice amplification capture;
Or second auxiliary microscope group light reflexed into the first reflective microscope group by switching device, the first reflective microscope group is by light Line reflection and reflexes to steering prism through the second reflective microscope group, the scattering by turning to prism reaches mesh to the first auxiliary microscope group Mirror, so as to user's observation.
Further, light supply apparatus is before the slice being placed on platform is illuminated further include:
Judge whether the slice is fluorescent staining slice or acid-fast stain slice;
If the slice is that fluorescent staining is sliced, light source switch controller closes light supply apparatus and opens fluorescent apparatus;
The fluorescent apparatus provides light source by the second reflective microscope group and objective lens for the slice on the platform;
Light is passed through objective lens and the reflective microscope group of third, the second auxiliary by the fluorescent staining part of the slice on the platform Microscope group is transferred to switching device.
In the present invention, a kind of micro- scan method is provided, light supply apparatus is that the slice being placed on platform is illuminated, light Line is reflected into the second auxiliary microscope group through the reflective microscope group of third by objective lens;Second auxiliary microscope group passes light by switching device It is handed to micro- camera, so that micro- camera carries out slice amplification capture by light;Or second auxiliary microscope group light is passed through Switching device reflexes to the first reflective microscope group, and light is reflexed to the first auxiliary microscope group by the first reflective microscope group, and reflective through second Microscope group reflexes to steering prism, and the scattering by turning to prism reaches eyepiece, so as to user's observation, so as to pass through switching dress It sets, so that micro- scanner not only can be used for carrying out slice scanning, but also can be used for carrying out microexamination, so that micro- scanner It can integrate slice scanning and two functions of microexamination, avoid realizing using the micro- scanner of boxlike and conventional microscope Two functions are stated, the working efficiency of micro- scanner is improved, reduces costs.
Detailed description of the invention
Fig. 1 is the light path schematic diagram of micro- scanner one embodiment provided by the invention;
Fig. 2 is the top view of micro- scanner provided by the invention;
Fig. 3 is the front view of micro- scanner provided by the invention;
Fig. 4 is the side view of micro- scanner provided by the invention;
Fig. 5 is the flow chart of micro- scan method one embodiment provided by the invention;
Fig. 6 is the flow chart of micro- another embodiment of scan method provided by the invention.
Appended drawing reference:
1- scanner ontology;2- eyepiece device;3- switching device;The reflective microscope group of 21- first;22- first assists microscope group; The reflective microscope group of 23- second;24- turns to prism;25- eyepiece;11- light supply apparatus;12- is used to place the platform of slice;13- object lens Group;The reflective microscope group of 14- third;15- second assists microscope group;The micro- camera of 16-;The reflective microscope group of 31- the 4th;The switching control of 32- lens Device processed;17- the first attachment lens group;4- fluorescent apparatus;5- light source switch controller;6- master controller.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art Every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.
Fig. 1 is the light path schematic diagram of micro- scanner one embodiment provided by the invention, as shown in Figure 1, comprising:
Scanner ontology 1, eyepiece device 2 and the switching device 3 for realizing optical path switching;Wherein,
Eyepiece device 2 includes: that the first reflective microscope group 21, first assists microscope group 22, the second reflective microscope group 23, turns to prism 24 With eyepiece 25;
Scanner ontology 1 includes: light supply apparatus 11, the platform 12 for placing slice, objective lens 13, the reflective microscope group of third 14, the second auxiliary microscope group 15 and micro- camera 16;
Wherein, auxiliary by light supply apparatus 11, the platform 12 for placing slice, objective lens 13, the reflective microscope group 14, second of third Microscope group 15, switching device 3, the first reflective microscope group 21, first is helped to assist microscope group 22, the second reflective microscope group 23, turn to 24 and of prism Eyepiece 25 forms the first optical path;
By light supply apparatus 11, the platform 12 for placing slice, objective lens 13, the reflective microscope group 14 of third, the second auxiliary mirror Group 15, switching device 3 and micro- camera 16 form the second optical path.
Wherein, scanner ontology 1 can also include: the first attachment lens group 17;It is mentioned referring to reference Fig. 2, Fig. 2 for the present invention The top view of the micro- scanner supplied.At this point, the second optical routing light supply apparatus, the platform for placing slice, objective lens, third Reflective microscope group, the second auxiliary microscope group, switching device, the first attachment lens group and micro- camera are formed.
Further, in conjunction with referring to figs. 2 and 3, Fig. 3 is the front view of micro- scanner provided by the invention, switching dress Setting 3 may include: the 4th reflective microscope group 31 and lens switch controller 32;Lens switch controller 32 and the 4th reflective microscope group 31 It is reflective to be reflexed to first for controlling the reflection angle of the 4th reflective microscope group 31 by connection for the light of second auxiliary microscope group 15 Microscope group 21 reaches eyepiece, or the light of the second auxiliary microscope group 15 is transferred to micro- camera 16.
Wherein, micro- scanner can also include: master controller 6, referring to Fig. 3, master controller 6 and lens switch controller 32 connections, for sending switching command to lens switch controller 32, so that lens switch controller 32 is according to switching command control Make the reflection angle of the 4th reflective microscope group 31.
Master controller can connect operation interface, so that user selects the first optical path or the second light in operation interface Road, if user selects the first optical path, master controller can inquire the reflection of the 4th reflective microscope group in the case of the first optical path of acquisition Reflection angle carrying is sent to lens switch controller, so that lens switch controller is according to cutting by angle in switching command Change the reflection angle of control the 4th reflective microscope group of control.Wherein, it under a kind of implement scene, can be set in the 4th reflective microscope group There is rotary shaft, lens switch controller may include: rotation axial brake device, and rotation axial brake device is rotated according to reflection angle Rotary shaft, so that rotary shaft drives the 4th reflective microscope group rotation, so that the 4th reflective microscope group reaches anti-in the case of the first optical path Firing angle degree.User selects operation when the second optical path similar, is no longer described in detail herein.
In addition, being also provided with panorama camera, panorama phase above platform in micro- scanner provided in this embodiment Machine can be connect with master controller, panorama camera can the quick capture under the control of master controller, the image of slice is passed through Master controller is shown in operation interface, so that user determines that slice is acid-fast stain slice or fluorescence according to the image of slice Stained slice, and then select the first optical path or the second optical path;Or master controller can be by sectioning image and the slice prestored Image is matched, and determines that slice is acid-fast stain slice or fluorescent staining slice, and then cut to lens according to matching result Changer controller sends switching command.
Further, in order to enable micro- scanner can be adapted for fluorescent staining slice and acid-fast stain slice, wherein The scanning of fluorescent staining slice scanning or observation that perhaps observation needs fluorescent apparatus acid-fast stain to be sliced need light source to fill It sets, referring to fig. 4, Fig. 4 is the side view of micro- scanner provided by the invention, and micro- scanner can also include: fluorescent apparatus 4 With light source switch controller 5.The optical circuit path between the reflective microscope group 14 of third and the second auxiliary microscope group 15 is arranged in fluorescent apparatus 4 On.Light source switch controller 5 is connect with fluorescent apparatus 4 and light supply apparatus 11 respectively, for controlling fluorescent apparatus 4 and light supply apparatus 11 open or close.
When slice is fluorescent staining slice, fluorescent apparatus 4 is opened, light supply apparatus 11 is closed.When slice is acid-fast stain When slice, light supply apparatus 11 is opened, closes fluorescent apparatus 4.
It needs to be illustrated, light source switch controller 5 may include: fluorescent light source controller and bright field light source control Device, fluorescent light source controller are connect with fluorescent apparatus, and bright field light source controller is connect with light supply apparatus, fluorescent light source controller and Bright field light source controller can be connected with the master controller outside micro- scanner, under the control of master controller, fluorescent light source Controller can control the opening and closing of fluorescent apparatus;Bright field light source controller can control unlatching and the pass of light supply apparatus It closes.
Wherein, the lower section of platform is arranged in light supply apparatus 11;The light emission direction of light supply apparatus 11 can be with object platform.When cutting When piece is that acid-fast stain is sliced, light supply apparatus is that the slice being placed on platform is illuminated, and light is by objective lens through third Reflective microscope group is reflected into the second auxiliary microscope group;Light is transferred to micro- camera by switching device by the second auxiliary microscope group, so that Micro- camera carries out slice amplification capture by light;Or second auxiliary microscope group light reflexed to first by switching device Reflective microscope group, light is reflexed to the first auxiliary microscope group by the first reflective microscope group, and reflexes to steering prism through the second reflective microscope group, Scattering by turning to prism reaches eyepiece, so as to user's observation.
The light emission direction of fluorescent apparatus 4 can be towards the reflective microscope group of third.When slice is fluorescent staining slice, third is anti- The light of fluorescent apparatus can be reflexed to objective lens by light microscopic group, and light is sent to the slice on platform by objective lens, be put down Light is transferred to switching by objective lens and the reflective microscope group of third, the second auxiliary microscope group by the fluorescent staining part of the slice on platform Device, and then reach eyepiece or micro- camera.
In the present embodiment, by providing a kind of micro- scanner, comprising: scanner ontology, eyepiece device and for realizing The switching device of optical path switching;Wherein, eyepiece device include: the first reflective microscope group, first auxiliary microscope group, the second reflective microscope group, Turn to prism and eyepiece;Scanner ontology includes: light supply apparatus, the platform for placing slice, objective lens, third reflective mirror Group, the second auxiliary microscope group and micro- camera;Wherein, reflective by light supply apparatus, the platform for placing slice, objective lens, third Microscope group, second auxiliary microscope group, switching device, the first reflective microscope group, first auxiliary microscope group, the second reflective microscope group, turn to prism and Eyepiece forms the first optical path;By light supply apparatus, the platform for placing slice, objective lens, the reflective microscope group of third, the second auxiliary mirror Group, switching device and micro- camera form the second optical path, so as to pass through switching device, so that micro- scanner can both be used In carrying out slice scanning, and it can be used for carrying out microexamination, so that micro- scanner can integrate slice scanning and micro- sight Two functions are examined, avoids realizing above-mentioned two function using the micro- scanner of boxlike and conventional microscope, improves micro- sweep The working efficiency for retouching instrument, reduces costs.
Fig. 5 is the flow chart of micro- scan method one embodiment provided by the invention, as shown in Figure 5, comprising:
501, light supply apparatus is that the slice being placed on platform is illuminated, and light is by objective lens through the reflective microscope group of third It is reflected into the second auxiliary microscope group.
502, light is transferred to micro- camera by switching device by the second auxiliary microscope group, so that micro- camera passes through light Carry out slice amplification capture.
Or 503, second auxiliary microscope group light reflexed into the first reflective microscope group, the first reflective microscope group by switching device Light is reflexed into the first auxiliary microscope group, and reflexes to steering prism through the second reflective microscope group, by turning to scattering to for prism Up to eyepiece, so as to user's observation.
Further, in conjunction with reference Fig. 6, light supply apparatus is before the slice being placed on platform is illuminated further include:
504, judge whether slice is fluorescent staining slice or acid-fast stain slice.
Wherein, judge whether slice is that fluorescent staining slice or acid-fast stain are sliced, it can be flat according to being located at by user The slice picture of panorama camera shooting above platform is judged or the slice picture of shooting can be sent to by panorama camera Master controller, master controller will be sliced picture and match with the slice picture prestored, to determine that slice is fluorescent staining slice Or acid-fast stain slice.
If 505, slice is that fluorescent staining is sliced, light source switch controller closes light supply apparatus and opens fluorescent apparatus.
506, fluorescent apparatus provides light source by the second reflective microscope group and objective lens for the slice on platform.
507, light is passed through objective lens and the reflective microscope group of third, the second auxiliary by the fluorescent staining part of the slice on platform Microscope group is transferred to switching device.
In the present embodiment, a kind of micro- scan method is provided, light supply apparatus is that the slice being placed on platform is illuminated, Light is reflected into the second auxiliary microscope group through the reflective microscope group of third by objective lens;Light is passed through switching device by the second auxiliary microscope group It is transferred to micro- camera, so that micro- camera carries out slice amplification capture by light;Or second auxiliary microscope group light is passed through It crosses switching device and reflexes to the first reflective microscope group, light is reflexed to the first auxiliary microscope group by the first reflective microscope group, and anti-through second Light microscopic group reflexes to steering prism, and the scattering by turning to prism reaches eyepiece, so as to user's observation, so as to pass through switching Device, so that micro- scanner not only can be used for carrying out slice scanning, but also can be used for carrying out microexamination, so that micro- scanning Instrument can integrate slice scanning and two functions of microexamination, avoid realizing using the micro- scanner of boxlike and conventional microscope Above-mentioned two function improves the working efficiency of micro- scanner, reduces costs.
Those of ordinary skill in the art will appreciate that: realize that all or part of the steps of above-mentioned each method embodiment can lead to The relevant hardware of program instruction is crossed to complete.Program above-mentioned can be stored in a computer readable storage medium.The journey When being executed, execution includes the steps that above-mentioned each method embodiment to sequence;And storage medium above-mentioned include: ROM, RAM, magnetic disk or The various media that can store program code such as person's CD.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations;To the greatest extent Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into Row equivalent replacement;And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution The range of scheme.

Claims (9)

1. a kind of micro- scanner characterized by comprising scanner ontology, eyepiece device and for realizing optical path switching Switching device;Wherein,
The eyepiece device includes: the first reflective microscope group, the first auxiliary microscope group, the second reflective microscope group, turns to prism and eyepiece;
The scanner ontology includes: light supply apparatus, the platform for placing slice, objective lens, the reflective microscope group of third, second auxiliary Help microscope group and micro- camera;
Wherein, by the light supply apparatus, for place slice platform, objective lens, the reflective microscope group of third, second auxiliary microscope group, Switching device, the first auxiliary microscope group, the second reflective microscope group, turns to prism and eyepiece the first optical path of formation at the first reflective microscope group;
By the light supply apparatus, the platform for placing slice, objective lens, the reflective microscope group of third, the second auxiliary microscope group, switching dress It sets and forms the second optical path with micro- camera;
Wherein, the switching device includes: the 4th reflective microscope group and lens switch controller;
The lens switch controller is connect with the 4th reflective microscope group, for controlling the angle of reflection of the 4th reflective microscope group The light of the second auxiliary microscope group is reflexed to the described first reflective microscope group and reaches eyepiece by degree, or auxiliary by described second The light of microscope group is helped to be transferred to the micro- camera.
2. micro- scanner according to claim 1, which is characterized in that the scanner ontology further include: the first auxiliary Lens group;
Wherein, by light supply apparatus, the platform for placing slice, objective lens, the reflective microscope group of third, the second auxiliary microscope group, switching Device, the first attachment lens group and micro- camera form the second optical path.
3. micro- scanner according to claim 1, which is characterized in that further include: fluorescent apparatus;
The fluorescent apparatus is arranged on the optical circuit path between the reflective microscope group of the third and the second auxiliary microscope group.
4. micro- scanner according to claim 3, which is characterized in that further include: light source switch controller;
The light source switch controller is connect with the fluorescent apparatus and the light supply apparatus respectively, for controlling the fluorescence dress It sets and is opened or closed with the light supply apparatus.
5. micro- scanner according to claim 1, which is characterized in that the light supply apparatus is arranged under the platform Side;
The light emission direction of the light supply apparatus is towards the platform.
6. micro- scanner according to claim 3, which is characterized in that the light emission direction of the fluorescent apparatus is towards described The reflective microscope group of third passes through institute so that the light of the fluorescent apparatus is reflexed to the objective lens by the reflective microscope group of the third State the slice that the light is sent on the platform by objective lens.
7. micro- scanner according to claim 1, which is characterized in that further include: master controller, the master controller with The lens switch controller connection, for sending switching command to the lens switch controller, so that the lens switch Controller controls the reflection angle of the 4th reflective microscope group according to switching command.
8. a kind of micro- scan method characterized by comprising
Light supply apparatus is that the slice being placed on platform is illuminated, and light is reflected into through the reflective microscope group of third by objective lens Two auxiliary microscope groups;
Light is transferred to micro- camera by switching device by the second auxiliary microscope group, so that micro- camera is sliced by light Amplify capture;
Or second auxiliary microscope group light reflexed into the first reflective microscope group by switching device, the first reflective microscope group is anti-by light It is incident upon the first auxiliary microscope group, and reflexes to steering prism through the second reflective microscope group, the scattering by turning to prism reaches eyepiece, with Just user observes;
Wherein, the switching device includes: the 4th reflective microscope group and lens switch controller;
The lens switch controller is connect with the 4th reflective microscope group, for controlling the angle of reflection of the 4th reflective microscope group The light of the second auxiliary microscope group is reflexed to the described first reflective microscope group and reaches eyepiece by degree, or auxiliary by described second The light of microscope group is helped to be transferred to the micro- camera.
9. micro- scan method according to claim 8, which is characterized in that light supply apparatus is the slice being placed on platform Before being illuminated further include:
Judge whether the slice is fluorescent staining slice or acid-fast stain slice;
If the slice is that fluorescent staining is sliced, light source switch controller closes light supply apparatus and opens fluorescent apparatus;
The fluorescent apparatus provides light source by the second reflective microscope group and objective lens for the slice on the platform;
Light is passed through objective lens and the reflective microscope group of third, the second auxiliary microscope group by the fluorescent staining part of the slice on the platform It is transferred to switching device.
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CN111122578B (en) * 2020-01-16 2024-01-26 宁波舜宇仪器有限公司 Material tray type pathological section scanner and section scanning method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105004723A (en) * 2015-06-25 2015-10-28 宁波江丰生物信息技术有限公司 Pathological section scanning 3D imaging and fusion device and method

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* Cited by examiner, † Cited by third party
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CN2921867Y (en) * 2006-07-25 2007-07-11 张路 Microscope visual frequency switching device
CN200962155Y (en) * 2006-10-08 2007-10-17 张前 A microscope forwarding device
CN102449528B (en) * 2009-05-27 2014-04-16 株式会社尼康 Microscope system
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CN104375268A (en) * 2014-11-26 2015-02-25 宁波江丰生物信息技术有限公司 Optical structure capable of carrying out bright field scanning and fluorescence scanning
CN205318017U (en) * 2015-12-28 2016-06-15 宁波舜宇仪器有限公司 Microscan appearance

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105004723A (en) * 2015-06-25 2015-10-28 宁波江丰生物信息技术有限公司 Pathological section scanning 3D imaging and fusion device and method

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