CN105483161A - Method for synthesizing CdS quantum dots in living cells - Google Patents
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Abstract
The invention discloses a method for synthesizing CdS quantum dots in living cells. The method is characterized in that cervical cancer HeLa cells are used as carriers, cell growth and metabolism activities are used as power, HeLa cell endogenous-substance glutathione is used as the stabilizer and protective agent, cadmium ions and sulfur ions are successively co-incubated with the HeLa cells to allow the cadmium ions and sulfur ions to react and nucleate under the drive of the cell growth and metabolism activities so as to generate the CdS quantum dots, the CdS quantum dots are processed by cell lysis, crushing, centrifuging and the like to obtain the CdS quantum dots stable in fluorescence and good in quality, the fluorescence emission wavelength of the CdS quantum dots is about 450 nanometers, the CdS quantum dots are of a cubic crystal structure, and the size of the quantum dots in an aqueous solution is about 3.0 nanometers. Compared with the prior art, the method has the advantages that the quantum dots synthesized by the method is low in toxicity, the biocompatibility of the quantum dots is increased greatly as compared with that of CdS quantum dots synthesized by traditional methods, and the quantum dots can be directly used for a bio-system.
Description
Technical field
The present invention relates to the synthetic method of CdS quantum dot in a kind of viable cell, belong to nano fluorescent material technical field.
Background technology
Quantum dot is the one of namo fluorescence probe, has excellent spectrum property, compared with organic fluorescent dye, is more suitable for medical science fluorescence imaging.In traditional chemical synthesis process, organo-metallic synthesis method due to organic reagent poisonous, synthesis condition is harsh, and needs to carry out complicated water-soluble processing links and just can apply in organism, brings difficulty to the further application of quantum dot.Aqueous phase synthesis method is simple to operate, favorable reproducibility, but the toxicity problem of cadmium content point is extremely disputed on always.In recent years, occurred that a large amount of use biological organism carries out the report of nano material synthesis for carrier.Large in these reports is carrier mainly with bacterium or fungi, by different conditions and method, has synthesized various types of quantum dots that biocompatibility is good.
Tumour cell is easy to cultivate, and propagation is very fast.There are some researches show, in tumour cell, some materials are if gsh equal size is apparently higher than normal cell, and gsh is excellent stablizer and protective material in quantum dot synthesis.Cell interior has various activities as growth, division etc., and these activities can promote interionic reacting to each other, and then regulates nucleation, the growth of particle.By certain density ion and cell being hatched altogether, making it enter in cell, under the environment of cell, with Growth of Cells Metabolic activity for power, promoting the synthesis of nanoparticle, a kind of novel, green synthetic method of can yet be regarded as.
HeLa cell is widely used in tumor research, Bioexperiment or cell cultures, has become very important instrument in medical research.This cell has following several large advantage: compared with other cancer cells, and proliferative abnormality is rapid, and cell strain can not be old and feeble lethal, and can divide without limit.
Summary of the invention
In order to overcome above-mentioned the deficiencies in the prior art, the invention provides the synthetic method of CdS quantum dot in a kind of viable cell, adopting with Cervical Cancer HeLa Cells is carrier, with Growth of Cells Metabolic activity for power, with HeLa endogenous cellular material gsh for stablizer and protective material, after cadmium ion and sulfonium ion are successively hatched altogether with HeLa cell, cadmium ion and sulfonium ion is made to be reacted into karyogenesis CdS quantum dot under the driving of metabolic activity in cells, pass through lysis again, broken, the serial of methods process such as centrifugal, obtained fluorescent stabilization, the CdS quantum dot of good properties, its fluorescence emission wavelengths is about 450nm, for cubic structure, in the aqueous solution, the size of quantum dot is about 3.0nm, compared with prior art, this quantum dot toxicity is little, and the quantum dot that biocompatibility synthesizes compared with traditional method improves greatly, can be directly used in living things system.
the present invention is achieved through the following technical solutions, the synthetic method of CdS quantum dot in a kind of viable cell, with cervical cancer cell HeLa cell for carrier, utilize cell life Metabolic activity to prepare quantum dot for power, concrete preparation process is as follows:
Step 1) cell cultures: HeLa cell is placed in the culturing bottle that perfect medium is housed, and put into incubator (37 DEG C, 5%CO
2) in hatch to cell density be 80% ~ 90%.
Step 2) cell and cadmium ion hatch altogether: take out the culturing bottle that step 1) is equipped with HeLa cell, substratum is abandoned in suction, after cleaning twice, HeLa cell with phosphate buffered saline buffer (PBS), what add new preparation contains cadmium-ion solution, its concentration of cadmium ions is 0.5 ~ 2.5 μm of ol/, 1,000,000 cells, be placed in incubator (37 DEG C, 5%CO
2) in hatch 2 ~ 6h, the obtained HeLa cell containing cadmium ion;
Described is add in perfect medium containing cadmic compound preparation containing cadmium-ion solution containing cadmium-ion solution, and first after through 0.45 μm and 0.22 μm of membrane filtration obtained.
Step 3) cell and sulfonium ion are hatched altogether: take out step 2) the obtained HeLa Tissue Culture Flask containing cadmium ion, substratum is abandoned in suction, after cleaning twice, HeLa cell with phosphate buffered saline buffer (PBS), add the sulfur-containing anion solution of new preparation, its sulfonium ion concentration is 0.5 ~ 2.0 μm of ol/, 1,000,000 cells, be placed in incubator (37 DEG C, 5%CO
2) in hatch 4 ~ 32h, the obtained HeLa cell containing CdS;
Described sulfur-containing anion solution is in perfect medium, add sulfocompound preparation sulfur-containing anion solution, and obtains through 0.45 μm and 0.22 μm of membrane filtration after elder generation.
Step 4) CdS quantum dot is extracted: taken out by the HeLa cell containing CdS with cell scraper, be transferred to centrifuge tube, the centrifugal 10min of 10000rpm, washes twice rear collecting cell with PBS; In collected cell, add 200 ~ 500 μ LRIPA lysates (by force), with biomixer by centrifugal to lysis 20 ~ 40min, 14000rpm 5min, get supernatant liquor.
Step 5) CdS quantum dot purifying and sign: in step 4) gained supernatant liquor, add Virahol (supernatant liquor and Virahol volume ratio are 1:1-3) and CdS quantum dot is precipitated, the centrifugal 5min of 3000rpm, gets precipitation; After repetition above-mentioned steps three times, namely precipitation is obtained quantum dot powder through lyophilize; Quantum dot dilutes through deionized water, surveys its spectrum and textural property.
Perfect medium described in each step is made up of 90%DMEM high glucose medium and 10% serum;
Consisting of of described DMEM high glucose medium: D-Glucose 4.5g/L, L-glutaminate 0.584g/L, sodium bicarbonate 3.7g/L, Sodium.alpha.-ketopropionate 0.110g/L, penicillin 80U/ml, Streptomycin sulphate 0.08mg/ml.
In described step 1), cell density is optimized for 80% ~ 85% further, and is in logarithmic phase.
Described step 2) in containing cadmic compound be Cadmium chloride fine powder or cadmium nitrate.
In described step 3), sulfocompound is sodium sulphite.
Described step 2), be placed in incubator (37 DEG C, 5%CO
2) in incubation time be optimized for 6h further.
Described step 3), be placed in incubator (37 DEG C, 5%CO
2) in incubation time be optimized for 12 ~ 30h further.
Described step 5) quantum dot adopts Virahol to precipitate, and the volume ratio of supernatant liquor and Virahol is optimized for 1:2 further.
Described step 2) in prepared by perfect medium containing cadmium-ion solution and sulfur-containing anion solution, and through the filter membrane filtration of 0.45 μm and 0.22 μm, object is to prevent cell microbiological contamination, affecting experimental result.
beneficial effect of the present invention is:
(1) utilizing cervical cancer cell HeLa cell for carrier, is that motivating force prepares CdS nano-quantum point with metabolic activity in cells.Owing to requiring higher for the bio-compatibility of the quantum dot be applied in organism, and in existing synthetic method, synthetic product is comparatively large to organismal toxicity, very easily damages organism.And the quantum dot of biological process synthesis synthesizes from cell, be applied to again in biology and go, its bio-compatibility also improves greatly, therefore, can reach the requirement of some medical treatment or diagnosis aspect.
(2) tumour cell endogenous material is utilized to synthesize quantum dot.Gsh is stablizer conventional in quantum dot building-up process and protective material.Correlative study shows, tumour cell Glutathione peptide content is apparently higher than normal cell.The growth of cell and fission process can promote the reaction that ion is mutual, and then regulate nucleation, the growth of particle.By certain density ion and cell being hatched altogether, it is made to enter cell, under the effect of intracellular environment, with Growth of Cells Metabolic activity for power can promote the synthesis of nanoparticle.
(3) simple to operate.Only need cadmium source and sulphur source successively to add in cell to hatch, without the need to special experiment condition, simply inexpensive.
(4) application is convenient.Because biocompatibility is higher, toxicity is less, so can be directly used in living things system.
Accompanying drawing explanation
Fig. 1 is the TEM figure of the CdS quantum dot of the embodiment of the present invention 2;
Fig. 2 is the CdS quantum dot XRD phenogram of the embodiment of the present invention 2.
embodiment:
Describe technical scheme of the present invention in detail below by concrete case study on implementation, but protection domain is not by this restriction.
embodiment 1: utilize Cadmium chloride fine powder to synthesize CdS quantum dot in HeLa cell
Concrete synthesis step is as follows:
Step 1) cell cultures: HeLa cell is placed in the culturing bottle that perfect medium is housed, and put into incubator (37 DEG C, 5%CO
2) in hatch to cell density to be 80%, and to be in logarithmic phase;
Step 2) cell and cadmium ion hatch altogether: take out the culturing bottle that step 1) is equipped with HeLa cell, substratum is abandoned in suction, after cleaning twice, HeLa cell with phosphate buffered saline buffer (PBS), what add new preparation contains cadmium-ion solution, its concentration of cadmium ions is 1.5 μm of ol/, 1,000,000 cells, be placed in incubator (37 DEG C, 5%CO
2) in hatch 6h, the obtained HeLa cell containing cadmium ion;
Described is in perfect medium, add Cadmium chloride fine powder preparation containing cadmium-ion solution containing cadmium-ion solution, and first after through 0.45 μm and 0.22 μm of membrane filtration obtained;
Step 3) cell and sulfonium ion are hatched altogether: take out step 2) the obtained HeLa Tissue Culture Flask containing cadmium ion, substratum is abandoned in suction, after cleaning twice, HeLa cell with phosphate buffered saline buffer (PBS), add the sulfur-containing anion solution of new preparation, its sulfonium ion concentration is 0.5 μm of ol/, 1,000,000 cells, be placed in incubator (37 DEG C, 5%CO
2) in hatch 12h, the obtained HeLa cell containing CdS quantum dot;
Described sulfur-containing anion solution is in perfect medium, add sodium sulphite preparation sulfur-containing anion solution, and obtains through 0.45 μm and 0.22 μm of membrane filtration after elder generation;
Step 4) CdS quantum dot is extracted: taken out by the HeLa cell containing CdS with cell scraper, be transferred to centrifuge tube, the centrifugal 10min of 10000rpm, washes twice rear collecting cell with PBS; In collected cell, add 200 μ LRIPA lysates (by force), with biomixer by centrifugal to lysis 20min, 14000rpm 5min, get supernatant liquor;
Step 5) CdS quantum dot purifying and sign: in step 4) gained supernatant liquor, add Virahol (supernatant liquor and Virahol volume ratio are 1:2) and CdS quantum dot is precipitated, the centrifugal 5min of 3000rpm, gets precipitation; After repetition above-mentioned steps three times, namely precipitation is obtained quantum dot powder through lyophilize; Quantum dot dilutes through deionized water, surveys its spectrum and textural property;
Described perfect medium is made up of 90%DMEM high glucose medium and 10% serum;
Consisting of of described DMEM high glucose medium: D-Glucose 4.5g/L, L-glutaminate 0.584g/L, sodium bicarbonate 3.7g/L, Sodium.alpha.-ketopropionate 0.110g/L, penicillin 80U/ml, Streptomycin sulphate 0.08mg/ml.
After testing, the CdS quantum dot emission wavelength of preparation is 451nm
embodiment 2: utilize Cadmium chloride fine powder to synthesize CdS quantum dot in HeLa cell
Concrete preparation process is as follows:
Step 1) cell cultures: HeLa cell is placed in the culturing bottle that perfect medium is housed, and put into incubator (37 DEG C, 5%CO
2) in hatch to cell density to be 85%, and to be in logarithmic phase;
Step 2) cell and cadmium ion hatch altogether: take out the culturing bottle that step 1) is equipped with HeLa cell, substratum is abandoned in suction, after cleaning twice, HeLa cell with phosphate buffered saline buffer (PBS), what add new preparation contains cadmium-ion solution, its concentration of cadmium ions is 1.0 μm of ol/, 1,000,000 cells, be placed in incubator (37 DEG C, 5%CO
2) in hatch 6h, the obtained HeLa cell containing cadmium ion;
Described is in perfect medium, add Cadmium chloride fine powder preparation containing cadmium-ion solution containing cadmium-ion solution, and first after through 0.45 μm and 0.22 μm of membrane filtration obtained;
Step 3) cell and sulfonium ion are hatched altogether: take out step 2) the obtained HeLa Tissue Culture Flask containing cadmium ion, substratum is abandoned in suction, after cleaning twice, HeLa cell with phosphate buffered saline buffer (PBS), add the sulfur-containing anion solution of new preparation, its sulfonium ion concentration is 1.0 μm of ol/, 1,000,000 cells, be placed in incubator (37 DEG C, 5%CO
2) in hatch 20h, the obtained HeLa cell containing CdS quantum dot;
Described sulfur-containing anion solution is in perfect medium, add sodium sulphite preparation sulfur-containing anion solution, and obtains through 0.45 μm and 0.22 μm of membrane filtration after elder generation;
Step 4) CdS quantum dot is extracted: taken out by the HeLa cell containing CdS with cell scraper, be transferred to centrifuge tube, the centrifugal 10min of 10000rpm, washes twice rear collecting cell with PBS; In collected cell, add 200 μ LRIPA lysates (by force), with biomixer by centrifugal to lysis 30min, 14000rpm 5min, get supernatant liquor;
Step 5) CdS quantum dot purifying and sign: in step 4) gained supernatant liquor, add Virahol (supernatant liquor and Virahol volume ratio are 1:2) and CdS quantum dot is precipitated, the centrifugal 5min of 3000rpm, gets precipitation; After repetition above-mentioned steps three times, namely precipitation is obtained quantum dot powder through lyophilize; Quantum dot dilutes through deionized water, surveys its spectrum and textural property, sees Fig. 1 and Fig. 2;
Described perfect medium is made up of 90%DMEM high glucose medium and 10% serum;
Consisting of of described DMEM high glucose medium: D-Glucose 4.5g/L, L-glutaminate 0.584g/L, sodium bicarbonate 3.7g/L, Sodium.alpha.-ketopropionate 0.110g/L, penicillin 80U/ml, Streptomycin sulphate 0.08mg/ml.
After testing, the CdS quantum dot emission wavelength of preparation is 454nm.
case study on implementation 3: utilize cadmium nitrate to synthesize CdS quantum dot in HeLa cell
Concrete preparation process is as follows:
Step 1) cell cultures: HeLa cell is placed in the culturing bottle that perfect medium is housed, and put into incubator (37 DEG C, 5%CO
2) in hatch to cell density be 85%;
Step 2) cell and cadmium ion hatch altogether: take out the culturing bottle that step 1) is equipped with HeLa cell, substratum is abandoned in suction, after cleaning twice, HeLa cell with phosphate buffered saline buffer (PBS), what add new preparation contains cadmium-ion solution, its concentration of cadmium ions is 2.0 μm of ol/, 1,000,000 cells, be placed in incubator (37 DEG C, 5%CO
2) in hatch 6h, the obtained HeLa cell containing cadmium ion;
Described is in perfect medium, add cadmium nitrate preparation containing cadmium-ion solution containing cadmium-ion solution, and first after through 0.45 μm and 0.22 μm of membrane filtration obtained;
Step 3) cell and sulfonium ion are hatched altogether: take out step 2) the obtained HeLa Tissue Culture Flask containing cadmium ion, substratum is abandoned in suction, after cleaning twice, HeLa cell with phosphate buffered saline buffer (PBS), add the sulfur-containing anion solution of new preparation, its sulfonium ion concentration is 1.5 μm of ol/, 1,000,000 cells, be placed in incubator (37 DEG C, 5%CO
2) in hatch 12h, the obtained HeLa cell containing CdS quantum dot;
Described sulfur-containing anion solution is in perfect medium, add sodium sulphite preparation sulfur-containing anion solution, and obtains through 0.45 μm and 0.22 μm of membrane filtration after elder generation;
Step 4) CdS quantum dot is extracted: taken out by the HeLa cell containing CdS with cell scraper, be transferred to centrifuge tube, the centrifugal 10min of 10000rpm, washes twice rear collecting cell with PBS; In collected cell, add 300 μ LRIPA lysates (by force), with biomixer by centrifugal to lysis 20min, 14000rpm 5min, get supernatant liquor;
Step 5) CdS quantum dot purifying and sign: in step 4) gained supernatant liquor, add Virahol (supernatant liquor and Virahol volume ratio are 1:3) and CdS quantum dot is precipitated, the centrifugal 5min of 3000rpm, gets precipitation; After repetition above-mentioned steps three times, namely precipitation is obtained quantum dot powder through lyophilize; Quantum dot dilutes through deionized water, surveys its spectrum and textural property;
Described perfect medium is made up of 90%DMEM high glucose medium and 10% serum;
Consisting of of described DMEM high glucose medium: D-Glucose 4.5g/L, L-glutaminate 0.584g/L, sodium bicarbonate 3.7g/L, Sodium.alpha.-ketopropionate 0.110g/L, penicillin 80U/ml, Streptomycin sulphate 0.08mg/ml.
After testing, the CdS quantum dot emission wavelength of preparation is 448nm.
case study on implementation 4: utilize cadmium nitrate to synthesize CdS quantum dot in HeLa cell
Concrete preparation process is as follows:
Step 1) cell cultures: HeLa cell is placed in the culturing bottle that perfect medium is housed, and put into incubator (37 DEG C, 5%CO
2) in hatch to cell density be 80%;
Step 2) cell and cadmium ion hatch altogether: take out the culturing bottle that step 1) is equipped with HeLa cell, substratum is abandoned in suction, after cleaning twice, HeLa cell with phosphate buffered saline buffer (PBS), what add new preparation contains cadmium-ion solution, its concentration of cadmium ions is 2.0 μm of ol/, 1,000,000 cells, be placed in incubator (37 DEG C, 5%CO
2) in hatch 6h, the obtained HeLa cell containing cadmium ion;
Described is add in perfect medium containing cadmic compound preparation containing cadmium-ion solution containing cadmium-ion solution, and first after through 0.45 μm and 0.22 μm of membrane filtration obtained;
Step 3) cell and sulfonium ion are hatched altogether: take out step 2) the obtained HeLa Tissue Culture Flask containing cadmium ion, substratum is abandoned in suction, after cleaning twice, HeLa cell with phosphate buffered saline buffer (PBS), add the sulfur-containing anion solution of new preparation, its sulfonium ion concentration is 2.0 μm of ol/, 1,000,000 cells, be placed in incubator (37 DEG C, 5%CO
2) in hatch 30h, the obtained HeLa cell containing CdS quantum dot;
Described sulfur-containing anion solution is in perfect medium, add sulfocompound preparation sulfur-containing anion solution, and obtains through 0.45 μm and 0.22 μm of membrane filtration after elder generation;
Step 4) CdS quantum dot is extracted: taken out by the HeLa cell containing CdS with cell scraper, be transferred to centrifuge tube, the centrifugal 10min of 10000rpm, washes twice rear collecting cell with PBS; In collected cell, add 500 μ LRIPA lysates (by force), with biomixer by centrifugal to lysis 20min, 14000rpm 5min, get supernatant liquor;
Step 5) CdS quantum dot purifying and sign: in step 4) gained supernatant liquor, add Virahol (supernatant liquor and Virahol volume ratio are 1:2) and CdS quantum dot is precipitated, the centrifugal 5min of 3000rpm, gets precipitation; After repetition above-mentioned steps three times, namely precipitation is obtained quantum dot powder through lyophilize; Quantum dot dilutes through deionized water, surveys its spectrum and textural property;
Described perfect medium is made up of 90%DMEM high glucose medium and 10% serum;
Consisting of of described DMEM high glucose medium: D-Glucose 4.5g/L, L-glutaminate 0.584g/L, sodium bicarbonate 3.7g/L, Sodium.alpha.-ketopropionate 0.110g/L, penicillin 80U/ml, Streptomycin sulphate 0.08mg/ml.
After testing, the CdS quantum dot emission wavelength of preparation is 452nm.
Claims (7)
1. the synthetic method of CdS quantum dot in viable cell, is characterized in that: with cervical cancer cell HeLa cell for carrier, and utilize cell life Metabolic activity to prepare CdS quantum dot for power, concrete preparation process is as follows:
Step 1) cell cultures: HeLa cell is placed in the culturing bottle that perfect medium is housed, and put into incubator (37 DEG C, 5%CO
2) in hatch to cell density be 80% ~ 90%;
Step 2) cell and cadmium ion hatch altogether: take out the culturing bottle that step 1) is equipped with HeLa cell, substratum is abandoned in suction, after cleaning twice, HeLa cell with phosphate buffered saline buffer (PBS), what add new preparation contains cadmium-ion solution, its concentration of cadmium ions is 0.5 ~ 2.5 μm of ol/, 1,000,000 cells, be placed in incubator (37 DEG C, 5%CO
2) in hatch 2 ~ 6h, the obtained HeLa cell containing cadmium ion;
Described is add in perfect medium containing cadmic compound preparation containing cadmium-ion solution containing cadmium-ion solution, and first after through 0.45 μm and 0.22 μm of membrane filtration obtained;
Step 3) cell and sulfonium ion are hatched altogether: take out step 2) the obtained HeLa Tissue Culture Flask containing cadmium ion, substratum is abandoned in suction, after cleaning twice, HeLa cell with phosphate buffered saline buffer (PBS), add the sulfur-containing anion solution of new preparation, its sulfonium ion concentration is 0.5 ~ 2.0 μm of ol/, 1,000,000 cells, be placed in incubator (37 DEG C, 5%CO
2) in hatch 4 ~ 32h, the obtained HeLa cell containing CdS;
Described sulfur-containing anion solution is in perfect medium, add sulfocompound preparation sulfur-containing anion solution, and obtains through 0.45 μm and 0.22 μm of membrane filtration after elder generation;
Step 4) CdS quantum dot is extracted: taken out by the HeLa cell containing CdS quantum dot with cell scraper, be transferred to centrifuge tube, the centrifugal 10min of 10000rpm, washes twice rear collecting cell with PBS; In collected cell, add 200 ~ 500 μ LRIPA lysates (by force), with biomixer by centrifugal to lysis 20 ~ 40min, 14000rpm 5min, get supernatant liquor;
Step 5) CdS quantum dot purifying and sign: in step 4) gained supernatant liquor, add Virahol (supernatant liquor and Virahol volume ratio are 1:1-3) and CdS quantum dot is precipitated, the centrifugal 5min of 3000rpm, gets precipitation; After repetition above-mentioned steps three times, namely precipitation is obtained quantum dot powder through lyophilize; Quantum dot dilutes through deionized water, surveys its spectrum and textural property;
Described perfect medium is made up of 90%DMEM high glucose medium and 10% serum;
Consisting of of described DMEM high glucose medium: D-Glucose 4.5g/L, L-glutaminate 0.584g/L, sodium bicarbonate 3.7g/L, Sodium.alpha.-ketopropionate 0.110g/L, penicillin 80U/ml, Streptomycin sulphate 0.08mg/ml.
2. the synthetic method of CdS quantum dot in a kind of viable cell according to claim 1, it is characterized in that: in described step 1), cell density is 80% ~ 85%, and is in logarithmic phase.
3. the synthetic method of CdS quantum dot in a kind of viable cell according to claim 1, is characterized in that: described step 2) in be Cadmium chloride fine powder or cadmium nitrate containing cadmic compound.
4. the synthetic method of CdS quantum dot in a kind of viable cell according to claim 1, is characterized in that: in described step 3), sulfocompound is sodium sulphite.
5. the synthetic method of CdS quantum dot in a kind of viable cell according to claim 1, is characterized in that: described step 2), be placed in incubator (37 DEG C, 5%CO
2) in hatch 6h.
6. the synthetic method of CdS quantum dot in a kind of viable cell according to claim 1, is characterized in that: described step 3), be placed in incubator (37 DEG C, 5%CO
2) in hatch 12 ~ 30h.
7. the synthetic method of CdS quantum dot in described viable cell according to claim 2, is characterized in that: described step 5) quantum dot adopts Virahol to precipitate, and the volume ratio of supernatant liquor and Virahol is 1:2.
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| CN106802290A (en) * | 2016-11-29 | 2017-06-06 | 武汉市宇驰检测技术有限公司 | A kind of fluorescence spectrophotometry that E. CoIi content is detected based on carbon quantum dot |
| CN111808889A (en) * | 2020-08-10 | 2020-10-23 | 北京理工大学 | Method for efficiently synthesizing fluorescence-adjustable ZnCdS quantum dots in vitro by using extracellular proteins |
| CN112973794A (en) * | 2021-03-08 | 2021-06-18 | 中国科学技术大学 | tetrahymena-CdS quantum dot intracellular hybridization system, construction method thereof and application thereof in visible light catalysis |
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| WO2012090161A1 (en) * | 2010-12-28 | 2012-07-05 | Universidad De Santiago De Chile | Synthesis of highly fluorescent gsh-cdte nanoparticles (quantum dots) |
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| CN106802290A (en) * | 2016-11-29 | 2017-06-06 | 武汉市宇驰检测技术有限公司 | A kind of fluorescence spectrophotometry that E. CoIi content is detected based on carbon quantum dot |
| CN111808889A (en) * | 2020-08-10 | 2020-10-23 | 北京理工大学 | Method for efficiently synthesizing fluorescence-adjustable ZnCdS quantum dots in vitro by using extracellular proteins |
| CN112973794A (en) * | 2021-03-08 | 2021-06-18 | 中国科学技术大学 | tetrahymena-CdS quantum dot intracellular hybridization system, construction method thereof and application thereof in visible light catalysis |
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