CN105445392A - Method for analyzing and detecting alkaline flavor components by using direct solvent extraction gas chromatography-mass spectrometry - Google Patents
Method for analyzing and detecting alkaline flavor components by using direct solvent extraction gas chromatography-mass spectrometry Download PDFInfo
- Publication number
- CN105445392A CN105445392A CN201510777330.6A CN201510777330A CN105445392A CN 105445392 A CN105445392 A CN 105445392A CN 201510777330 A CN201510777330 A CN 201510777330A CN 105445392 A CN105445392 A CN 105445392A
- Authority
- CN
- China
- Prior art keywords
- flavor component
- sample
- alkaline
- tobacco
- add
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/025—Gas chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plural Heterocyclic Compounds (AREA)
Abstract
The invention discloses a method for analyzing and detecting alkaline flavor components by using direct solvent extraction gas chromatography-mass spectrometry. The method is characterized by comprising the following specific steps: 1) accurately weighing a tobacco waste sample, adding a sodium hydroxide solution and an internal standard solution, soaking and performing ultrasonic extraction; 2) adding a dichloromethane extraction solvent into the supernate, repeatedly extracting for three times and collecting and merging the dichloromethane phase; adding anhydrous sodium sulfate, vibrating and then standing; performing rotary evaporation to 1mL; and filtering with a 0.45-micron filter membrane to obtain a to-be-detected sample; and 3) detecting the sample with a GC-MS instrument; and after the detection, treating the atlas data to obtain an analysis test result of alkaline flavor components. The method disclosed by the invention has the following advantages: the pretreatment process is simple, mild and fast; the alkaline flavor components in tobacco can be accurately tested; and the method is of important theoretical significance and application value in directing the scientific cigarette formula and improving the cigarette flavor and taste.
Description
Technical field
The invention belongs to tobacco flavor composition detection technical field, be specifically related to the method that the analysis of a kind of direct solvent extraction gas phase hydrolysis and condensation detects tobacco neutral and alkali flavor component.
Background technology
Tobacco flavor composition is one of principal element affecting tobacco and cigarette quality, is the important indicator evaluating tobacco product aesthetic quality.Tobacco flavor composition roughly can be divided into acid flavor component, neutral flavor constituents, alkaline flavor component.Tobacco neutral and alkali flavor component mainly comprises the heterocycle compounds such as pyridine, pyrazine, pyrroles, quinoline, they are fragrance matters that in tobacco, a class is important, although their content in tobacco is very micro-, but because they have very strong smell and lower threshold value, make them play an important role in enhancing tobacco aroma characteristic.
The steam distillation of sample and organic solvent extraction combine by Simultaneous distillation-extraction (SDE), and qualitative, quantitative is effective, are flavor component extracting method in the most frequently used at present tobacco.Although the Sample Pretreatment Technique that SDE generally adopts in tobacco flavor constituent analysis, but also can not following problem be there is with avoiding in SDE: (1) Simultaneous distillation-extraction is a unlimited system, long-time high-temperature heating distillation under air ambient and atmospheric pressure, the chemical reactions such as some active high alkaline flavor components can be oxidized, thermal degradation.Create the composition do not had in raw sample on the one hand, original component portion scatters and disappears on the other hand, causes error to analysis result; (2) vapo(u)rizing temperature is high, and the alkaline flavor component that boiling point is lower can scatter and disappear, but water solution system causes vapo(u)rizing temperature again can not be too high, and the alkaline flavor component that boiling point is too high but can not distill; (3) alkaline components that content is very low is difficult to quantitatively.Therefore, research simple, gentle, fast separation and concentration pre-treating method seem necessary.
Therefore, under setting up normal temperature alkaline flavor component extracting process and can Accurate Determining tobacco neutral and alkali flavor component by the method for gas chromatography combined with mass spectrometry technical Analysis test tobacco neutral and alkali flavor component, to instructing cigarette scientific formula, improve cigarette flavor and taste quality there is important theory significance and using value.
Summary of the invention
Object of the present invention provides a kind of just and analyzes the method detecting tobacco neutral and alkali flavor component based on above-mentioned prior art situation.
The object of the invention is to be achieved through the following technical solutions:
The analysis of direct solvent extraction gas phase hydrolysis and condensation detects a method for tobacco neutral and alkali flavor component, comprises following concrete steps:
1) accurately take 10-25g offal sample, add the sodium hydroxide solution of 100-300mL1%-20%, add inner mark solution, soak 15min, ultrasonic extraction 60min, centrifugally obtain supernatant;
2) pour supernatant into separating funnel, add 100mL dichloromethane extraction solvent, shake up rear stratification, release lower floor's methylene chloride phase, re-extract three times, collect combined dichloromethane phase; Add 20g anhydrous sodium sulfate, leave standstill 3 hours after vibration, rotary evaporation, to 1mL, joins chromatogram bottle after filtering, obtain detected sample with 0.45 micron membrane filter;
3) GC-MS instrument is adopted to step 2) gained sample detects.Detect complete, spectrum data is processed to the analytical test result obtaining alkaline flavor component.
Alkaline flavor component of the present invention comprises pyridine and its derivatives (as 3-picoline, 4-picoline, 2-acetylpyridine, 2-pyridine carboxaldehyde, 3-pyridine carboxaldehyde, 2; 3-dipyridine etc.), pyrazine and derivant thereof be (as 2-methylpyrazine, 2; 5-dimethyl pyrazine, 2,6-dimethyl pyrazines), pyrroles and derivant (as 2-acetyl pyrrole, 2-pyrrole aldehyde), quinoline.
The chromatographic condition detected described in step (3) is: chromatographic column: DB-Innowaxetr (60m × 0.32mmi.d. × 0.50 μm d.f.); Carrier gas: He; Flow velocity: 1.0ml/min; Injector temperature: 250 DEG C; Sample size: 1 μ L; Split ratio 10:1; Solvent delay 10min; Transmission line temperature: 250 DEG C; Temperature programme: 60 DEG C are raised to 230 DEG C with 2 DEG C/min, retains 30min.
The Mass Spectrometry Conditions detected described in step (3) is: ionization mode: EI; Ion source temperature: 230 DEG C; Ionizing energy: 70eV; Scan mode: SCAN and SIM scans simultaneously, retention time and quota ion are in table 1.
Retention time, the quota ion of the alkaline flavor component of table 1.
| Numbering | Compound | RT（min） | ION |
| 1 | Pyridine | 17.32 | 79 |
| 2 | Pyrazine | 18.92 | 80 |
| 3 | 2-methylpyrazine | 22.00 | 94 |
| 4 | 3-picoline | 23.74 | 93 |
| 5 | 4-picoline | 24.19 | 93 |
| 6 | 2,5-dimethyl pyrazine | 25.29 | 108 |
| 7 | 2,6-dimethyl pyrazine | 25.63 | 108 |
| 8 | Pyrroles | 37.41 | 67 |
| 9 | 2-pyridine carboxaldehyde | 39.74 | 79 |
| 10 | 2-acetylpyridine | 42.74 | 79 |
| 11 | 3-pyridine carboxaldehyde | 48.78 | 107 |
| 12 | Quinoline | 61.46 | 129 |
| 13 | 2-acetyl pyrrole | 63.02 | 109 |
| 14 | 2-pyrrole aldehyde | 65.89 | 95 |
| 15 | 2,3-dipyridine | 87.28 | 156 |
Detection in step 3) adopts internal standard quanitation, hybrid standard working solution is marked and 15 kinds of alkaline flavor component objects in a kind, methylene chloride, the concentration 10 μ g/mL of mark deuterated pyridine in each concentration hybrid standard working solution, and often kind of alkaline flavor component concentration is the same, hybrid standard working solution neutral and alkali flavor component concentration magnitude is followed successively by 0.1 μ g/mL, 0.5 μ g/mL, 5 μ g/mL, 10 μ g/mL, 50 μ g/mL, 100 μ g/mL, 200 μ g/mL from low to high.
The linear equation of 15 kinds of alkaline flavor components, the range of linearity, detection limit are as shown in table 2.。
Linear equation, the range of linearity, the detection limit of table 215 kind of alkaline flavor component
The present invention soaks with sodium hydroxide solution and alkaline flavor component is dissociated out from tobacco, directly extract with methylene chloride again, this pre-treating method carries out at normal temperatures, treatment conditions are gentle, do not destroy the chemical constitution of alkaline flavor component, and prevent the volatilization of low-boiling flavor component to run off, therefore analysis result is more true and reliable than present Simultaneous distillation-extraction method.Therefore, this method has Pretreatment gentleness, simple to operate, the analysis result advantage such as accurately and reliably.
Embodiment
Below by embodiment, the present invention is further described:
Embodiment 1
1) accurately take 15g flue-cured tobacco offal sample, add the sodium hydroxide solution of 100mL10%, add 10 μ g inner mark solutions, soak 15min, ultrasonic extraction 60min, centrifugally obtain supernatant.
2) pour supernatant into separating funnel, add 100mL dichloromethane extraction solvent, shake up rear stratification, release lower floor's methylene chloride phase.Re-extract three times, collects methylene chloride phase.Add 20g anhydrous sodium sulfate, after vibration, leave standstill 3 hours.Rotary evaporation, to 1mL, joins chromatogram bottle after filtering, obtain detected sample with 0.45 micron membrane filter.
3) GC-MS instrument is adopted to step 2) gained sample detects.Detect complete, spectrum data is processed to the analytical test result obtaining alkaline flavor component.
Embodiment 2
1) accurately take 25g flue-cured tobacco offal sample, add the sodium hydroxide solution of 200mL15%, add 10 μ g inner mark solutions, soak 15min, ultrasonic extraction 60min, centrifugally obtain supernatant.
2) pour supernatant into separating funnel, add 100mL dichloromethane extraction solvent, shake up rear stratification, release lower floor's methylene chloride phase.Re-extract three times, collects methylene chloride phase.Add 20g anhydrous sodium sulfate, after vibration, leave standstill 3 hours.Rotary evaporation, to 1mL, joins chromatogram bottle after filtering, obtain detected sample with 0.45 micron membrane filter.
3) GC-MS instrument is adopted to step 2) gained sample detects.Detect complete, spectrum data is processed to the analytical test result obtaining alkaline flavor component.
Embodiment 3
1) accurately take 20g burley tobaccos offal sample, add the sodium hydroxide solution of 300mL20%, add 10 μ g inner mark solutions, soak 15min, ultrasonic extraction 60min, centrifugally obtain supernatant.
2) pour supernatant into separating funnel, add 100mL dichloromethane extraction solvent, shake up rear stratification, release lower floor's methylene chloride phase.Re-extract three times, collects methylene chloride phase.Add 20g anhydrous sodium sulfate, after vibration, leave standstill 3 hours.Rotary evaporation, to 1mL, joins chromatogram bottle after filtering, obtain detected sample with 0.45 micron membrane filter.
3) GC-MS instrument is adopted to step 2) gained sample detects.Detect complete, spectrum data is processed to the analytical test result obtaining alkaline flavor component.
The testing result of embodiment is in table 3.
The alkaline flavor component test result of table 3
| Numbering | Compound | Embodiment 1(μ g/g) | Embodiment 2(μ g/g) | Embodiment 3(μ g/g) |
| 1 | Pyridine | 1.0025 | 0.9045 | 1.2256 |
| 2 | Pyrazine | 0.1380 | 0.1412 | 0.1356 |
| 3 | 2-methylpyrazine | 2.3294 | 2.6527 | 2.4678 |
| 4 | 3-picoline | 0.03373 | 0.04620 | 0.03837 |
| 5 | 4-picoline | 0.04214 | 0.04288 | 0.04782 |
| 6 | 2,6-dimethyl pyrazine | 0.9254 | 0.9025 | 0.09373 |
| 7 | 2,6-dimethyl pyrazine | 1. 3710 | 1.2937 | 1.2235 |
| 8 | Pyrroles | 0.4944 | 0.4748 | 0.4125 |
| 9 | 2-pyridine carboxaldehyde | 1.5085 | 1.7732 | 1.6324 |
| 10 | 2-acetylpyridine | 0.4652 | 0.4255 | 0.4872 |
| 11 | 3-pyridine carboxaldehyde | 0.7978 | 0.7061 | 0.8012 |
| 12 | Quinoline | 0.2438 | 0.3333 | 0.3680 |
| 13 | 2-acetyl pyrrole | 6.3098 | 6.0385 | 6.2185 |
| 14 | 2-pyrrole aldehyde | 0.9483 | 0.8898 | 0.9600 |
| 15 | 2,3-dipyridine | 7.2158 | 6.9587 | 8.6524 |
Claims (4)
1. the analysis of direct solvent extraction gas phase hydrolysis and condensation detects a method for tobacco neutral and alkali flavor component, it is characterized in that: comprise following concrete steps:
1) accurately take 10-25g offal sample, add the sodium hydroxide solution of 100-300mL1%-20%, add inner mark solution, soak 15min, ultrasonic extraction 60min, centrifugally obtain supernatant;
2) pour supernatant into separating funnel, add 100mL dichloromethane extraction solvent, shake up rear stratification, release lower floor's methylene chloride phase, re-extract three times, collect combined dichloromethane phase; Add 20g anhydrous sodium sulfate, leave standstill 3 hours after vibration, rotary evaporation, to 1mL, joins chromatogram bottle after filtering, obtain detected sample with 0.45 micron membrane filter;
3) GC-MS instrument is adopted to step 2) gained sample detects; Detect complete, spectrum data is processed to the analytical test result obtaining alkaline flavor component;
Chromatographic condition is: chromatographic column: DB-Innowaxetr, specification 60m × 0.32mmi.d. × 0.50 μm d.f; Carrier gas: He; Flow velocity: 1.0ml/min; Injector temperature: 250 DEG C; Sample size: 1 μ L; Split ratio 10:1; Solvent delay 10min; Transmission line temperature: 250 DEG C; Temperature programme: 60 DEG C are raised to 230 DEG C with 2 DEG C/min, retains 30min;
Mass Spectrometry Conditions is: ionization mode: EI; Ion source temperature: 230 DEG C; Ionizing energy: 70eV; Scan mode: SCAN and SIM scans simultaneously.
2. the method analyzing detection tobacco neutral and alkali flavor component according to claim 1, it is characterized in that: be designated as deuterated pyridine in described, the concentration of inner mark solution is 10 μ g/mL, and solvent is methylene chloride.
3. the method analyzing detection tobacco neutral and alkali flavor component according to claim 1, it is characterized in that: the detection in step 3) adopts internal standard quanitation, hybrid standard working solution is marked and 15 kinds of alkaline flavor component objects in a kind, methylene chloride, the concentration 10 μ g/mL of mark deuterated pyridine in each concentration hybrid standard working solution, and often kind of alkaline flavor component concentration is the same, standard working solution neutral and alkali flavor component concentration magnitude is followed successively by 0.1 μ g/mL from low to high, 0.5 μ g/mL, 5 μ g/mL, 10 μ g/mL, 50 μ g/mL, 100 μ g/mL, 200 μ g/mL.
4. the method analyzing detection tobacco neutral and alkali flavor component according to claim 1; it is characterized in that: described alkaline flavor component comprises following 15 kinds: pyridine, 3-picoline, 4-picoline, 2-acetylpyridine, 2-pyridine carboxaldehyde, 3-pyridine carboxaldehyde, 2; 3-dipyridine, pyrazine, 2-methylpyrazine, 2; 5-dimethyl pyrazine, 2,6-dimethyl pyrazines, pyrroles, 2-acetyl pyrrole, 2-pyrrole aldehyde, quinoline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510777330.6A CN105445392A (en) | 2015-11-13 | 2015-11-13 | Method for analyzing and detecting alkaline flavor components by using direct solvent extraction gas chromatography-mass spectrometry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510777330.6A CN105445392A (en) | 2015-11-13 | 2015-11-13 | Method for analyzing and detecting alkaline flavor components by using direct solvent extraction gas chromatography-mass spectrometry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105445392A true CN105445392A (en) | 2016-03-30 |
Family
ID=55555831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510777330.6A Pending CN105445392A (en) | 2015-11-13 | 2015-11-13 | Method for analyzing and detecting alkaline flavor components by using direct solvent extraction gas chromatography-mass spectrometry |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105445392A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105929082A (en) * | 2016-07-14 | 2016-09-07 | 云南中烟工业有限责任公司 | Method for separating and determining pyrazine substances and pyridine substances in saliva |
| CN109212062A (en) * | 2018-09-07 | 2019-01-15 | 安徽瑞邦生物科技有限公司 | A method of with nicotinonitrile and 3- picoline in HPLC detection industrial wastewater |
| CN110082467A (en) * | 2019-04-12 | 2019-08-02 | 中国检验检疫科学研究院 | Detect the method and kit of exogenous N- methyl -2- pyrrole aldehyde |
| CN110542736A (en) * | 2019-10-14 | 2019-12-06 | 云南省烟草农业科学研究院 | Method for simultaneously analyzing 9 alkaloids and 11 aroma components in tobacco leaves |
| CN113203823A (en) * | 2021-04-21 | 2021-08-03 | 中国烟草总公司郑州烟草研究院 | Application of hydroxyl-containing compound, matrix improver and method for analyzing flavor components in tobacco |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002022075A2 (en) * | 2000-09-18 | 2002-03-21 | Electro-Optics Research & Development Ltd. | Odors discrimination based on spectral signatures |
| CN101261254A (en) * | 2008-05-08 | 2008-09-10 | 中国烟草总公司郑州烟草研究院 | Determination method for transfer behaviors index of alkaline spices in cigarette |
| CN102766534A (en) * | 2012-07-16 | 2012-11-07 | 上海烟草集团有限责任公司 | Method for separating alkaline scent ingredients from main stream smoke of cigarettes and application |
| CN105044264A (en) * | 2015-07-10 | 2015-11-11 | 云南中烟工业有限责任公司 | Method for accurate characterization and evaluation of characteristics of baking aroma in cigarette aroma |
-
2015
- 2015-11-13 CN CN201510777330.6A patent/CN105445392A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002022075A2 (en) * | 2000-09-18 | 2002-03-21 | Electro-Optics Research & Development Ltd. | Odors discrimination based on spectral signatures |
| CN101261254A (en) * | 2008-05-08 | 2008-09-10 | 中国烟草总公司郑州烟草研究院 | Determination method for transfer behaviors index of alkaline spices in cigarette |
| CN102766534A (en) * | 2012-07-16 | 2012-11-07 | 上海烟草集团有限责任公司 | Method for separating alkaline scent ingredients from main stream smoke of cigarettes and application |
| CN105044264A (en) * | 2015-07-10 | 2015-11-11 | 云南中烟工业有限责任公司 | Method for accurate characterization and evaluation of characteristics of baking aroma in cigarette aroma |
Non-Patent Citations (4)
| Title |
|---|
| L.Q. SHENG 等: "Determination of nicotine-related alkaloids in tobacco and cigarette smoke by GC-FID", 《CHROMATOGRAPHIA》 * |
| SUDHIR KUMAR PANDEY 等: "A review of environmental tobacco smoke and its determination in air", 《TRENDS IN ANALYTICAL CHEMISTRY》 * |
| 崔韬 等: "不同类型烟叶中碱性香味成分的分析研究", 《农产品加工(学刊)》 * |
| 陈顺辉 等: "施氮水平对烤后烟叶酸性和碱性致香物质含量的影响", 《中国农学通报》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105929082A (en) * | 2016-07-14 | 2016-09-07 | 云南中烟工业有限责任公司 | Method for separating and determining pyrazine substances and pyridine substances in saliva |
| CN109212062A (en) * | 2018-09-07 | 2019-01-15 | 安徽瑞邦生物科技有限公司 | A method of with nicotinonitrile and 3- picoline in HPLC detection industrial wastewater |
| CN110082467A (en) * | 2019-04-12 | 2019-08-02 | 中国检验检疫科学研究院 | Detect the method and kit of exogenous N- methyl -2- pyrrole aldehyde |
| CN110542736A (en) * | 2019-10-14 | 2019-12-06 | 云南省烟草农业科学研究院 | Method for simultaneously analyzing 9 alkaloids and 11 aroma components in tobacco leaves |
| CN110542736B (en) * | 2019-10-14 | 2022-05-06 | 云南省烟草农业科学研究院 | Method for simultaneously analyzing 9 alkaloids and 11 aroma components in tobacco leaves |
| CN113203823A (en) * | 2021-04-21 | 2021-08-03 | 中国烟草总公司郑州烟草研究院 | Application of hydroxyl-containing compound, matrix improver and method for analyzing flavor components in tobacco |
| CN113203823B (en) * | 2021-04-21 | 2024-05-31 | 中国烟草总公司郑州烟草研究院 | Application of hydroxyl-containing compound, matrix improver and analysis method of flavor components in tobacco |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105445392A (en) | Method for analyzing and detecting alkaline flavor components by using direct solvent extraction gas chromatography-mass spectrometry | |
| CN105241994A (en) | Method for analyzing and detecting alkaline aroma constituents in tobacco through ultrasound-assisted cloud-point extraction gas chromatography-mass spectrometry technology | |
| CN102004132B (en) | Method for measuring alkaloid in tobacco and tobacco product | |
| Shen et al. | Determination of tobacco alkaloids by gas chromatography–mass spectrometry using cloud point extraction as a preconcentration step | |
| US20130239981A1 (en) | Method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves | |
| CN104820040A (en) | Method for measuring nicotine content in gum base type chewing tobacco | |
| CN104569197B (en) | The silanization GC/MS detection method of sugar, 1,2-propylene glycol and the glycerol in mensuration Nicotiana tabacum L. simultaneously | |
| CN105467055A (en) | Method for determining alkaloids in tea leaves by using GC-MS (Gas Chromatography-Mass Spectrometer) method | |
| CN106353419B (en) | Method for measuring flavor components in cigarette mainstream smoke | |
| CN105158392A (en) | Method for determining special tobacco N-nitrosamine in cigarette liquid of electronic cigarette | |
| CN106556665B (en) | The method for measuring 14 kinds of acid flavor component separatelies in cigarette mainstream flue gas simultaneously | |
| Kubica et al. | An evaluation of sucrose as a possible contaminant in e-liquids for electronic cigarettes by hydrophilic interaction liquid chromatography–tandem mass spectrometry | |
| Sakuma et al. | The distribution of cigarette smoke components between mainstream and sidestream smoke: II. Bases | |
| CN101113972A (en) | Method for detecting ammonia trapped by cigarette filter tip by ion chromatography conductance | |
| Edinboro et al. | Determination of aflatoxin B1 in sidestream cigarette smoke by immunoaffinity column extraction coupled with liquid chromatography/mass spectrometry | |
| CN109085270A (en) | Method that is a kind of while measuring a variety of tobacco-specific nitrosamines in human plasma | |
| CN104535694A (en) | Method for detecting four tobacco-specific nitrosamines (TSNAs) in lateral exhaust gas of cigarettes by virtue of gas chromatography-tandem mass spectrometry | |
| Mitsui et al. | LC fractionation followed by pyrolysis GC–MS for the in-depth study of aroma compounds formed during tobacco combustion | |
| CN101261254B (en) | Determination method for transfer behaviors index of alkaline spices in cigarette | |
| CN106770795A (en) | 14 kinds of methods of the fragrant and sweet composition of Jiao in heating non-burning cigarette smoke are determined simultaneously | |
| CN108548880B (en) | Method for measuring various components in electronic cigarette smoke | |
| CN104165950B (en) | The detection method of formaldehyde in a kind of hot melt adhesive | |
| CN103149297B (en) | High performance liquid chromatography-mass spectrometry combined detection method for -NNAL and (R) -NNAL in urine | |
| CN107490642A (en) | Method that is a kind of while determining 12 kinds of alkaloids in cigarette smoke granule phase substance | |
| CN105784886A (en) | Gas chromatography-tandem mass spectrum detection method for hydrazine in cigarette mainstream smoke |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160330 |
|
| WD01 | Invention patent application deemed withdrawn after publication |