CN105241994A - Method for analyzing and detecting alkaline aroma constituents in tobacco through ultrasound-assisted cloud-point extraction gas chromatography-mass spectrometry technology - Google Patents
Method for analyzing and detecting alkaline aroma constituents in tobacco through ultrasound-assisted cloud-point extraction gas chromatography-mass spectrometry technology Download PDFInfo
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Abstract
The invention discloses a method for analyzing and detecting alkaline aroma constituents in tobacco through an ultrasound-assisted cloud-point extraction gas chromatography-mass spectrometry technology. The method includes the steps that 1, a tobacco powder sample is accurately weighed, a sodium hydroxide solution and an internal standard solution are added, soaking and ultrasonic extraction are performed, centrifugation is performed to obtain supernate, a surfactant is added, the mixture is evenly stirred to form a 5%-15% solution, the 5%-15% solution is heated to a temperature higher than the cloud point temperature, and an aqueous phase is removed after standing layering or centrifugal layering; 2, reextraction solvent is added, ultrasonic extraction is performed, a reextraction solvent phase on the upper layer is taken, anhydrous sodium sulfate is added, the mixture is oscillated and then subjected to standing concentration, and the product is filtered through a filtration membrane to obtain a sample to be detected; 3, the sample is detected through a GC-MS, and graph spectrum data are processed to obtain an analysis and test result of the alkaline aroma constituents. The method has the advantages that the pretreatment process is simple, mild and fast, the alkaline aroma constituents in the tobacco can be accurately determined, and the method has great theoretical significance and application value for guiding a scientific formula of cigarettes and improving the aroma and taste quality of the cigarettes.
Description
Technical field
The invention belongs to tobacco flavor composition detection technical field, be specifically related to a kind of method that ultrasonic wave added cloud point extraction gas chromatography combined with mass spectrometry technical Analysis detects tobacco neutral and alkali flavor component.
Background technology
Tobacco flavor composition is one of principal element affecting tobacco and cigarette quality, is the important indicator evaluating tobacco product aesthetic quality.Tobacco flavor composition roughly can be divided into acid flavor component, neutral flavor constituents, alkaline flavor component.Tobacco neutral and alkali flavor component mainly comprises the heterocycle compounds such as pyridine, pyrazine, pyrroles, quinoline, they are fragrance matters that in tobacco, a class is important, although their content in tobacco is very micro-, but because they have very strong smell and lower threshold value, make them play an important role in enhancing tobacco aroma characteristic.
The pre-treatment separation method that tobacco flavor constituent analysis is commonly used mainly contains steam distillation, organic solvent extraction, head space separation, head space condistillation, Simultaneous distillation-extraction and solid-phase microextraction etc.The steam distillation of sample and organic solvent extraction combine by Simultaneous distillation-extraction (SDE), and qualitative, quantitative is effective, are flavor component extracting method in the most frequently used at present tobacco.Although the Sample Pretreatment Technique that SDE generally adopts in tobacco flavor constituent analysis, but also can not following problem be there is with avoiding in SDE: (1) Simultaneous distillation-extraction is a unlimited system, long-time high-temperature heating distillation under air ambient and atmospheric pressure, the chemical reactions such as some active high alkaline flavor components can be oxidized, thermal degradation.Create the composition do not had in raw sample on the one hand, original component portion scatters and disappears on the other hand, causes error to analysis result; (2) vapo(u)rizing temperature is high, and the alkaline flavor component that boiling point is lower can scatter and disappear, but water solution system causes vapo(u)rizing temperature again can not be too high, and the alkaline flavor component that boiling point is too high but can not distill; (3) alkaline components that content is very low is difficult to quantitatively.Therefore, research simple, gentle, fast separation and concentration pre-treating method seem necessary.
The micellar aqueous solution of some surfactants is heated or cooled and occurs turbid phenomenon to during uniform temperature because initiation is separated, i.e. cloud point phenomenon, place a period of time or centrifugal after be divided into two-phase: one is surfactant phase (accounting for 5% of cumulative volume); Another is aqueous phase (micellar concentration equals critical micelle concentration).The lyophobic dust dissolved in the solution is combined with the hydrophobic grouping of surfactant, and be extracted and enter surfactant phase, hydrophilic substance then stays aqueous phase.If impose contrary temperature action, then two-phase disappears, and again forms uniform solution.The temperature that this phenomenon is occurred is exactly cloud point temperature, the method be separated based on the realization of this phenomenon is exactly cloud point extraction (cloudpointextraction, CPE), also micella medium extraction (micellarmediatedextraction, MME) is claimed.
CPE, as a kind of emerging abstraction technique, has extraction conditions gentleness, dosage of surfactant is less, applied range, extraction efficiency are high, enrichment factor is large, low price, system composition is simple, surfactant is easily separated with target compound, surfactant is recyclable, environmental protection (not with an organic solvent), easily and the plurality of advantages such as postorder instrument analytical method coupling such as high performance liquid chromatography (HPLC), gas chromatography (GC), Flow Injection Analysis (FIA).
Therefore, the new method setting up ultrasonic wave added cloud point extraction gas chromatography combined with mass spectrometry technical Analysis test tobacco neutral and alkali flavor component for instructing cigarette scientific formula, improve cigarette flavor and taste quality there is important theory significance and using value.
Summary of the invention
Object of the present invention provides a kind of method utilizing Cloud-Point Extraction Technique analysis to detect tobacco neutral and alkali flavor component just based on above-mentioned prior art situation.
The object of the invention is to be achieved through the following technical solutions:
Ultrasonic wave added cloud point extraction gas chromatography combined with mass spectrometry technical Analysis detects a method for tobacco neutral and alkali flavor component, comprises following concrete steps:
1) 10-25g offal sample is accurately taken, add the sodium hydroxide solution of 100-200mL1%-20%, add inner mark solution, power 40-80w ultrasonic extraction 10-60min is used after soaking 15min, centrifugally obtain supernatant, add 5-30mL and stir and form the solution of 5%-15%, be heated above surfactant cloud point temperature 10-20 DEG C, keep this temperature 20-60min, aqueous phase discarded after stratification or centrifugal layering.
2) add stripping solvent methylene chloride 10-50mL, ultrasonic extraction 10min, stratification or centrifugal layering, get upper strata stripping solvent phase, add anhydrous sodium sulfate, leave standstill 2h after vibration, be concentrated into 1mL, join chromatogram bottle after filtering with 0.45 micron membrane filter, obtain detected sample
3) GC-MS instrument is adopted to step 2) gained sample detects, detects complete, spectrum data is processed to the analytical test result obtaining alkaline flavor component.
Alkaline flavor component of the present invention comprises pyridine and its derivatives (as 3-picoline, 4-picoline, 2-acetylpyridine, 2-pyridine carboxaldehyde, 3-pyridine carboxaldehyde etc.), pyrazine and derivant thereof (as 2-methylpyrazine, 2; 5-dimethyl pyrazine, 2,6-dimethyl pyrazines), pyrroles and derivant (as 2-acetyl pyrrole, 2-pyrrole aldehyde), quinoline.
Described in step (1), surfactant comprises: polyoxyethylene fatty alcohol (Brij30, Brij35, Brij56, GenapolX-080 etc.), to tertiary octyl phenyl polyglycol ether (TritonX-114, TritonX-100 etc.), n-alkyl-phenyl polyglycol ether (PONPE7.5, PONPE10 etc.).
Described in step (1), inner mark solution is deuterated pyridine, and the concentration of inner mark solution is 10 μ g/mL, and solvent is methylene chloride.
Described in step (2), stripping solvent is methylene chloride.
The chromatographic condition detected described in step (3) is: chromatographic column: DB-Innowaxetr (60m × 0.32mmi.d. × 0.50 μm d.f.); Carrier gas: He; Flow velocity: 1.0ml/min; Injector temperature: 250 DEG C; Sample size: 1 μ L; Segregation ratio 10:1; Solvent delay 10min; Transmission line temperature: 250 DEG C; Temperature programme: 60 DEG C are raised to 230 DEG C with 2 DEG C/min, retains 30min.
The Mass Spectrometry Conditions detected described in step (3) is: ionization mode: EI; Ion source temperature: 230 DEG C; Ionizing energy: 70eV; Scan mode: SCAN and SIM scans simultaneously, retention time and quota ion are in table 1.
Retention time, the quota ion of the alkaline flavor component of table 1.
| Numbering | Compound | RT（min） | ION |
| 1 | Pyridine | 17.32 | 79 |
| 2 | Pyrazine | 18.92 | 80 |
| 3 | 2-methylpyrazine | 22.00 | 94 |
| 4 | 3-picoline | 23.74 | 93 |
| 5 | 4-picoline | 24.19 | 93 |
| 6 | 2,5-dimethyl pyrazine | 25.29 | 108 |
| 7 | 2,6-dimethyl pyrazine | 25.63 | 108 |
| 8 | Pyrroles | 37.41 | 67 |
| 9 | 2-pyridine carboxaldehyde | 39.74 | 79 |
| 10 | 2-acetylpyridine | 42.74 | 79 |
| 11 | 3-pyridine carboxaldehyde | 48.78 | 107 |
| 12 | Quinoline | 61.46 | 129 |
| 13 | 2-acetyl pyrrole | 63.02 | 109 |
| 14 | 2-pyrrole aldehyde | 65.89 | 95 |
Detection in step 3) adopts internal standard quanitation, hybrid standard working solution is marked and 14 kinds of alkaline flavor component objects in a kind, methylene chloride, the concentration 10 μ g/mL of mark deuterated pyridine in each concentration hybrid standard working solution, and often kind of alkaline flavor component concentration is the same, hybrid standard working solution neutral and alkali flavor component concentration magnitude is followed successively by 0.1 μ g/mL, 0.5 μ g/mL, 5 μ g/mL, 10 μ g/mL, 50 μ g/mL, 100 μ g/mL, 200 μ g/mL from low to high.
The linear equation of 14 kinds of alkaline flavor components, the range of linearity, detection limit are as shown in table 2.
Linear equation, the range of linearity, the detection limit of table 214 kind of alkaline flavor component
The present invention soaks with sodium hydroxide solution and alkaline flavor component is dissociated out from tobacco, utilize the cloud point phenomenon of surfactant, realize the separation and concentration of alkaline flavor component, strip mutually from surfactant with methylene chloride again, this pre-treating method carries out at normal temperatures, treatment conditions are gentle, do not destroy the chemical constitution of alkaline flavor component, and prevent the volatilization of low-boiling flavor component to run off, have concentration effect to alkaline flavor component, therefore analysis result is more true and reliable than present Simultaneous distillation-extraction method simultaneously.Therefore, this method has Pretreatment gentleness, simple to operate, the range of linearity is wide, detection limit is low, the analysis result advantage such as accurately and reliably.
Embodiment
Below by embodiment, the present invention is further described:
embodiment 1
1) accurately take 15g offal sample, add the sodium hydroxide solution of 100mL10%, add 10 μ g inner mark solutions, soak with power 40w ultrasonic extraction 60min after 15min, centrifugally obtain supernatant.Add 10mLGenapolX-080 to stir the surfactant solution of formation 10%, be heated to 55 DEG C, keep this temperature 20min.Lower floor's aqueous phase is discarded after stratification.
2) stripping solvent 10mL is added, ultrasonic extraction 10min.Upper strata stripping solvent phase is got in centrifugal layering, adds 2g anhydrous sodium sulfate, leaves standstill 2h after vibration.Be concentrated into 1mL, join chromatogram bottle after filtering with 0.45 micron membrane filter, obtain detected sample.
3) GC-MS instrument is adopted to step 2) gained sample detects.Detect complete, spectrum data is processed to the analytical test result obtaining alkaline flavor component.
embodiment 2
1) accurately take 25g offal sample, add the sodium hydroxide solution of 200mL5%, add 10 μ g inner mark solutions, soak with power 60w ultrasonic extraction 30min after 15min, centrifugally obtain supernatant.Add 20mLTritonX-100 to stir the surfactant solution of formation 10%, be heated to 85 DEG C, keep this temperature 30min.Lower floor's aqueous phase is discarded after stratification.
2) stripping solvent 30mL is added, ultrasonic extraction 10min.Upper strata stripping solvent phase is got in centrifugal layering, adds 3g anhydrous sodium sulfate, leaves standstill 2h after vibration.Be concentrated into 1mL, join chromatogram bottle after filtering with 0.45 micron membrane filter, obtain detected sample.
3) GC-MS instrument is adopted to step 2) gained sample detects.Detect complete, spectrum data is processed to the analytical test result obtaining alkaline flavor component.
embodiment 3
1) accurately take 20g offal sample, add the sodium hydroxide solution of 150mL20%, add 10 μ g inner mark solutions, soak with power 80w ultrasonic extraction 10min after 15min, centrifugally obtain supernatant.Add 7.5mLPONPE10 to stir the solution of formation 5%, be heated above cloud point temperature 45 DEG C, keep this temperature 30min.Lower floor's aqueous phase is discarded after centrifugal layering.
2) stripping solvent 50mL is added, ultrasonic extraction 10min.Upper strata stripping solvent phase is got in centrifugal layering, adds 5g anhydrous sodium sulfate, leaves standstill 2h after vibration.Be concentrated into 1mL, join chromatogram bottle after filtering with 0.45 micron membrane filter, obtain detected sample.
3) GC-MS instrument is adopted to step 2) gained sample detects.Detect complete, spectrum data is processed to the analytical test result obtaining alkaline flavor component.
The testing result of embodiment is in table 3.
The alkaline flavor component test result of table 3
| Numbering | Compound | Embodiment 1(μ g/g) | Embodiment 2(μ g/g) | Embodiment 3(μ g/g) |
| 1 | Pyridine | 1.1322 | 0.9055 | 1.2250 |
| 2 | Pyrazine | 0.1680 | 0.1011 | 0.1588 |
| 3 | 2-methylpyrazine | 2.5291 | 2.2526 | 2.4651 |
| 4 | 3-picoline | 0.03672 | 0.05621 | 0.04837 |
| 5 | 4-picoline | 0.04311 | 0.03289 | 0.04582 |
| 6 | 2,6-dimethyl pyrazine | 0.8254 | 0.9524 | 0.09275 |
| 7 | 2,6-dimethyl pyrazine | 1. 2712 | 1.2237 | 1.2538 |
| 8 | Pyrroles | 0.3940 | 0.4540 | 0.4022 |
| 9 | 2-pyridine carboxaldehyde | 1.2089 | 1.8732 | 1.5321 |
| 10 | 2-acetylpyridine | 0.3653 | 0.3250 | 0.3872 |
| 11 | 3-pyridine carboxaldehyde | 0.6968 | 0.7967 | 0.8054 |
| 12 | Quinoline | 0.2538 | 0.3532 | 0.2988 |
| 13 | 2-acetyl pyrrole | 5.3091 | 5.0382 | 5.2145 |
| 14 | 2-pyrrole aldehyde | 0.8487 | 0.5487 | 0.7689 |
Claims (5)
1. ultrasonic wave added cloud point extraction gas chromatography combined with mass spectrometry technical Analysis detects a method for tobacco neutral and alkali flavor component, it is characterized in that: comprise following concrete steps:
1) 10-25g offal sample is accurately taken, add the sodium hydroxide solution of 100-200mL1%-20%, add inner mark solution, soak with power 40-80w ultrasonic extraction 10-60min after 15min, centrifugally obtain supernatant, add 5-30mL surfactant, stir and form the solution of 5%-15%, be heated above surfactant cloud point temperature 10-20 DEG C, keep this temperature 20-60min, aqueous phase discarded after stratification or centrifugal layering;
2) add stripping solvent methylene chloride 10-50mL, ultrasonic extraction 10min, stratification or centrifugal layering, get upper strata stripping solvent phase, add anhydrous sodium sulfate, leave standstill 2h after vibration, be concentrated into 1mL, join chromatogram bottle after filtering with 0.45 micron membrane filter, obtain detected sample
3) GC-MS instrument is adopted to step 2) gained sample detects, detects complete, spectrum data is processed to the analytical test result obtaining alkaline flavor component,
Chromatographic condition is: chromatographic column: DB-Innowaxetr, specification 60m × 0.32mmi.d. × 0.50 μm d.f.; Carrier gas: He; Flow velocity: 1.0ml/min; Injector temperature: 250 DEG C; Sample size: 1 μ L; Split ratio 10:1; Solvent delay 10min; Transmission line temperature: 250 DEG C; Temperature programme: 60 DEG C are raised to 230 DEG C with 2 DEG C/min, retains 30min;
Mass Spectrometry Conditions is: ionization mode: EI; Ion source temperature: 230 DEG C; Ionizing energy: 70eV; Scan mode: SCAN and SIM scans simultaneously.
2. according to claim 1ly analyze the method detecting tobacco neutral and alkali flavor component, it is characterized in that: described surfactant comprises: polyoxyethylene fatty alcohol, to tertiary octyl phenyl polyglycol ether, n-alkyl-phenyl polyglycol ether.
3. according to claim 1ly analyze the method detecting tobacco neutral and alkali flavor component, it is characterized in that described in be designated as deuterated pyridine, the concentration of inner mark solution is 10 μ g/mL, and solvent is methylene chloride.
4. the method analyzing detection tobacco neutral and alkali flavor component according to claim 1, it is characterized in that: the detection in step 3) adopts internal standard quanitation, hybrid standard working solution is marked and 14 kinds of alkaline flavor component objects in a kind, methylene chloride, the concentration 10 μ g/mL of mark deuterated pyridine in each concentration hybrid standard working solution, and often kind of alkaline flavor component concentration is the same, hybrid standard working solution neutral and alkali flavor component concentration magnitude is followed successively by 0.1 μ g/mL from low to high, 0.5 μ g/mL, 5 μ g/mL, 10 μ g/mL, 50 μ g/mL, 100 μ g/mL, 200 μ g/mL.
5. the method analyzing detection tobacco neutral and alkali flavor component according to claim 1; it is characterized in that: described alkaline flavor component comprises following 14 kinds: pyridine, 3-picoline, 4-picoline, 2-acetylpyridine, 2-pyridine carboxaldehyde, 3-pyridine carboxaldehyde, pyrazine, 2-methylpyrazine, 2; 5-dimethyl pyrazine, 2,6-dimethyl pyrazines, pyrroles, 2-acetyl pyrrole, 2-pyrrole aldehyde, quinoline.
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| CN106053619A (en) * | 2016-05-11 | 2016-10-26 | 中国烟草总公司郑州烟草研究院 | A high-throughput analysis method for measuring volatile and semi-volatile components in particulate matters of cigarette main stream smoke |
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| CN106290639A (en) * | 2016-08-11 | 2017-01-04 | 云南中烟工业有限责任公司 | A kind of quantitative forecasting technique of Medicated cigarette milk flavoured type |
| CN106770793A (en) * | 2017-01-20 | 2017-05-31 | 烟台出入境检验检疫局检验检疫技术中心 | The method that QNS in breast or dairy products is detected using cloud point extraction liquid chromatography tandem mass spectrometry |
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