CN105441225B

CN105441225B - Bio-instruments pipeline-cleaning liquid and preparation method

Info

Publication number: CN105441225B
Application number: CN201510949070.6A
Authority: CN
Inventors: 王国涛; 詹海燕; 余铭恩; 吴琼杉; 王璐; 朱伟; 项美华
Original assignee: Hangzhou Beaconlab Biotechnology Co Ltd
Current assignee: Hangzhou Beaconlab Biotechnology Co Ltd
Priority date: 2015-12-18
Filing date: 2015-12-18
Publication date: 2019-01-29
Anticipated expiration: 2035-12-18
Also published as: CN105441225A

Abstract

The present invention relates to a kind of bio-instruments pipeline-cleaning liquid that can be dissolved and remove protein molecular accompanying by pipeline inner wall and preparation methods, mass percent: trishydroxymethylaminomethane (Tris) accounts for 0.5%~1% as ingredient I, beta-cyclodextrin accounts for 0.2%~0.5% as ingredient II, ε-poly-D-lysine accounts for 0.2%~0.5% as ingredient III, NaTDC accounts for 0.5% ~ 1% as ingredient IV, and purified water is solvent as ingredient V and accounts for 97%~98.6%.Advantage: first is that obvious to the protein residue cleaning effect in plastics, ceramics, metal, glass piping, after cleaning, pipeline inner surface does not hang droplet, and no liquid residual will not impact subsequent pipeline use；Second is that it is non-corrosive to plastics, ceramics, metal, glass piping, it overcomes through damage of the conventional methods to pipeline inner wall such as soda acid cleanings；Third is that using safe, to human body table fanout free region, operational risk is not present in this cleaning solution；Fourth is that this cleaning solution is not limited to the cleaning to various material channel interiors, it can also be used to the cleaning of various hard surfaces.

Description

Bio-instruments pipeline-cleaning liquid and preparation method

Technical field

Protein molecular accompanying by pipeline inner wall can be dissolved and remove the present invention relates to a kind of, such as enzyme-linked immunosorbent assay institute The metal spraying used in coating machine, colloidal gold immunochromatographimethod production draws the bio-instruments pipeline of the instruments such as film instrument, protein purification instrument Cleaning solution and preparation method belong to bio-instruments microfluidic control field.

Background technique

With the development that biotechnology is maked rapid progress, the application field of bio-instruments is also more and more extensive, but is related to egg The bio-instruments of white microfluidic control, it is relatively high to required precision, it is very sensitive to pollutant, and it is whether the smoothness of its pipeline and residual The attachment of albumen is stayed to will have a direct impact on its precision and the subsequent effect used.Remaining tiny bubble will affect in pipeline in pipeline The pressure balance in portion, and then influence liquid precision out；And protein residue can pollute instrument subsequent use, directly affect reality Test the accuracy of result.

Be traditionally used for cleaning bio-instruments pipeline cleaning solution there are two main classes: the first kind is soda acid substance, is passed through The addition of soda acid, and repeated flushing, are denaturalized residual protein, lose bioactivity, tie to avoid the subsequent use of pipeline to experiment The influence of fruit, but the based cleaning liquid has some shortcomings: first, soda acid cleaning can cause to corrode to metal tubes, can break The bright and clean degree of bad glass piping inner surface is used for a long time the accelerated ageing for also resulting in plastic piping, causes pipeline leakproofness It is bad；, can not be fully erased second, although soda acid can allow bioprotein molecular inactivation, over time, albumen be cumulative, The aperture that fine pipeline can be blocked causes drain unsmooth；Third, after being cleaned with soda acid needing that soda acid will be remained with purified water It cleans up, after purified water is emptied, pipeline inner surface is since surface tension can remain droplet, subsequent in use, residual Droplet energy diluted protein concentration, cause initial drain protein concentration inaccuracy, and then influence experimental result.Second class is highly concentrated The surfactant of degree, although concentrated surfactant temporarily can effectively remove the bubble generated inside pipeline, and energy The most of remaining protein molecular of dissolution, but for obstinate protein adsorption (being such as insoluble in the albumen of water), it is ineffective.? After surfactant washing pipeline, it is still desirable to purified water repeated flushing pipeline until removing surfactant residual, At this point, some obstinate protein residues still result in reappearing for bubble.

Summary of the invention

Purpose of design: avoiding the shortcoming in background technique, and designing a kind of can effectively remove bio-instruments pipeline inner wall The cleaning solution of protein residue, after cleaning, pipeline inner wall will not generate bubble without protein residue, and not hang droplet, to rear Continuous pipeline uses pollution-free, bio-instruments pipeline-cleaning liquid and preparation method on experimental result without influence.

Design scheme: 1. beta-cyclodextrins have hole at the center of cyclic structure, and there are-CH- and glycoside knot in inside The oxygen atom of conjunction, it is aqueous in sulphur, 2 position of glucose, 3 and 6-OH base be in hydrophily, can pass through faint Van der Waals force will Protein molecular is complexed into inclusion compound, increases its dissolubility.2. ε-poly-D-lysine is rich in positive charge, with the object for having negative electrical charge Matter has strong electrostatic force, and has good penetration power to protein film, can remove protein film from pipeline inner surface.3. NaTDC is a kind of ionic detergent, can more especially be insoluble in the albumen of water, such as memebrane protein with soluble protein. 4. trishydroxymethylaminomethane (Tris) provides alkaline environment, the stable state of beta-cyclodextrin can be maintained, while can prevent ε-poly-D-lysine degradation and stabilized deoxy sodium taurocholate.5. or more substance synergistic effect, can effectively dissolve and remove pipeline The bioprotein molecule of inner surface absorption, avoids the cross contamination in the subsequent use process of pipeline.

Technical solution: a kind of bio-instruments pipeline-cleaning liquid, containing trishydroxymethylaminomethane (Tris), beta-cyclodextrin, ε-poly-D-lysine, NaTDC, purified water, and trishydroxymethylaminomethane (Tris), beta-cyclodextrin, ε-poly rely ammonia Acid, NaTDC, five kinds of ingredients of purified water mass percentage content be respectively as follows: trishydroxymethylaminomethane (Tris) conduct Ingredient I accounts for 0.5%~1%, and beta-cyclodextrin accounts for 0.2%~0.5%, ε-poly-D-lysine as ingredient III as ingredient II and accounts for 0.2%~ 0.5%, NaTDC accounts for 0.5% ~ 1% as ingredient IV, and purified water is solvent as ingredient V and accounts for 97%~98.6%.

Compared with the background technology, the present invention, first is that this cleaning solution is residual to the albumen in plastics, ceramics, metal, glass piping Stay cleaning effect obvious, after cleaning, pipeline inner surface does not hang droplet, and no liquid residual will not cause subsequent pipeline use It influences；Second is that this cleaning solution is non-corrosive to plastics, ceramics, metal, glass piping, overcome through tradition sides such as soda acid cleanings Damage of the method to pipeline inner wall, especially to the destruction of metal pipeline internal surface；Third is that this cleaning solution is using safe, to human body table Operational risk is not present in fanout free region；Fourth is that this cleaning solution is not limited to the cleaning to various material channel interiors, it can also be used to The cleaning of various hard surfaces.

Specific embodiment

Embodiment 1: the mass percentage content of novel washing liquid each component is respectively as follows: trishydroxymethylaminomethane (Tris) 0.5%~1% is accounted for as ingredient I, beta-cyclodextrin accounts for 0.2%~0.5%, ε-poly-D-lysine as ingredient III as ingredient II and accounts for 0.2%~0.5%, NaTDC accounts for 0.5%~1% as ingredient IV, and purified water is solvent as ingredient V and accounts for 97%~98.6%.

Embodiment 2: on the basis of embodiment 1, trishydroxymethylaminomethane (Tris) accounts for 0.75, β-ring as ingredient I Dextrin accounts for 0.3%, ε-poly-D-lysine as ingredient III as ingredient II and accounts for 0.25%, and NaTDC is accounted for as ingredient IV 0.75%, purified water is solvent as ingredient V and accounts for 97.95%.

Embodiment 3: on the basis of embodiment 1, trishydroxymethylaminomethane (Tris) accounts for 0.5%, β-ring as ingredient I Dextrin accounts for 0.4%, ε-poly-D-lysine as ingredient III as ingredient II and accounts for 0.3%, and NaTDC accounts for 0.8% as ingredient IV, Purified water is solvent as ingredient V and accounts for 98%.

Embodiment 4: on the basis of embodiment 1, trishydroxymethylaminomethane (Tris) accounts for 0.5%, β-ring as ingredient I Dextrin accounts for 0.5%, ε-poly-D-lysine as ingredient III as ingredient II and accounts for 0.2%, and NaTDC accounts for 0.5% as ingredient IV, Purified water is solvent as ingredient V and accounts for 98.3%.

Embodiment 5: on the basis of the above embodiments, as described in right 3, trishydroxymethylaminomethane is first weighed (Tris), beta-cyclodextrin, ε-poly-D-lysine, NaTDC mixing, are dissolved in water, and stir evenly later, are sub-packed in close It seals in vial.

Embodiment 6: on the basis of embodiment 5, the application method of bio-instruments pipeline-cleaning liquid, it is characterized in that: according to Cleaning solution is retained in pipeline 1 later with the cleaning solution flushing pipeline 3 times by the included cleaning procedure of bio-instruments used Hour, it then empties, then with purified water detergent line 10 times.

It is to be understood that: although above-described embodiment retouches mentality of designing of the invention detailed text of contrasting It states, but these verbal descriptions, only the simple text of mentality of designing of the present invention is described, rather than to mentality of designing of the present invention Limitation, any combination, increase or modification without departing from mentality of designing of the present invention falls within the protection scope of the present invention.

Claims

1. a kind of bio-instruments pipeline-cleaning liquid, it is characterized in that by trishydroxymethylaminomethane (Tris), beta-cyclodextrin, ε-poly Five kinds of lysine, NaTDC, purified water ingredients are constituted, and trishydroxymethylaminomethane (Tris), beta-cyclodextrin, ε-are more The mass percentage content that five kinds of polylysine, NaTDC, purified water ingredients are constituted is respectively as follows: trishydroxymethylaminomethane (Tris) 0 .5%~1% is accounted for as ingredient I, beta-cyclodextrin accounts for the .5% of 0 .2%~0, ε-poly-D-lysine conduct as ingredient II Ingredient III accounts for the .5% of 0 .2%~0, and NaTDC accounts for 0 .5% ~ 1% as ingredient IV, and purified water is that solvent is accounted for as ingredient V 97%~98 .6%；Its preparation method: it first weighs trishydroxymethylaminomethane (Tris), beta-cyclodextrin, ε-poly-D-lysine, take off The mixing of oxycholic acid sodium, is dissolved in water, and stir evenly later, is sub-packed in sealed glass jars.

2. a kind of application method of bio-instruments pipeline-cleaning liquid as described in claim 1, it is characterized in that: according to biology used Cleaning solution is retained in 1 hour in pipeline later, then with the cleaning solution flushing pipeline 3 times by the included cleaning procedure of instrument Emptying, then with purified water detergent line 10 times.