CN105424784A - Microfluidic chip for detecting heavy metal ions in water and detection method - Google Patents
Microfluidic chip for detecting heavy metal ions in water and detection method Download PDFInfo
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Abstract
The invention belongs to the field of environmental protection, and particularly relates to a microfluidic chip for detecting heavy metal ions in water and a detection method. The microfluidic chip comprises micro-channels and micro-valves, wherein the micro-channels comprise a reference micro-channel, an ion imprint micro-channel, an electrophoresis sample introduction micro-channel, an electrophoresis separation micro-channel, a connection micro-channel, a waste liquor exhaustion micro-channel and a buffer solution exhaustion micro-channel; the micro-valves comprise a reference end outlet micro-valve, an ion imprint exit micro-valve, a waste liquor exit micro-valve, a buffer solution cut-off micro-valve and a sampling cut-off micro-valve. The detection method comprises the following steps: acquiring a first electropherogram of cross electrophoresis before heavy metal ion adsorption; then, acquiring a second electropherogram of cross electrophoresis after heavy metal ion adsorption; finally, recognizing specific heavy metal ions according to the difference between the two electropherograms, and calculating heavy metal contents. The microfluidic chip and the detection method have the advantages that not only are the production cost and the use cost very low, but also the anti-interference ability is very high; moreover, the detection process is simple, and the detection time is short.
Description
Technical field
The invention belongs to field of environment protection, be specifically related to detection of heavy metal ion micro-fluidic chip and method in a kind of water.
Background technology
Heavy metal ion in water body not only can destroy ecologic environment, and can threaten health of human body, therefore, is necessary to detect the heavy metal ion in water.Tradition detection of heavy metal ion equipment comprises the checkout equipment based on colourimetry, the checkout equipment based on atomic absorption spectrography (AAS) and the checkout equipment etc. based on bio-sensing method, these legacy equipments have played vital role in detection of heavy metal ion field, but, in the process used, find that these legacy equipments also have following problem:
The first, because conventional detection devices has been combined by multiple component, integrated level is low, and volume is large, quality is large, power consumption is large therefore to make these checkout equipments have, and three " greatly " problem adds production cost and the use cost of equipment;
The second, because some conventional detection devices only pays close attention to heavy metal ion to be detected, lack and the function setting shielded is disturbed to other, therefore there iing multiple heavy metal ion and depositing or have other pollutant to deposit in case, these checkout equipments will reduce the identity of target heavy metal ion, have impact on detection order of accuarcy;
Three, because some conventional detection devices is before detection heavy metal ion, need preprocessing process, therefore testing process relative complex, detection time is long;
Four, the reagent strip used due to classic method is toxic, therefore
Overcome the above problems, detection of heavy metal ion equipment development will be made to a new height.
Summary of the invention
For above problem, the invention discloses detection of heavy metal ion micro-fluidic chip and detection method in a kind of water, the present invention not only has very low production cost and use cost, and has very strong antijamming capability, testing process is simple simultaneously, and detection time is short.
The object of the present invention is achieved like this:
Detection of heavy metal ion micro-fluidic chip in a kind of water, comprises microchannel and micro-valve,
Described microchannel comprises with reference to microchannel, ion blotting microchannel, electrophoresis sample introduction microchannel, electrophoretic separation microchannel, connects microchannel, waste liquid discharge microchannel and damping fluid discharge microchannel;
Described micro-valve comprises with reference to bringing out the micro-valve of mouth, ion blotting exports micro-valve, the micro-valve of waste liquid outlet, damping fluid end micro-valve and sample introduction ends micro-valve;
Set gradually electrophoresis sample introduction microchannel and electrophoretic separation microchannel by damping fluid flow direction, described electrophoresis sample introduction microchannel and electrophoretic separation microchannel form cross electrophoresis;
Be set up in parallel with reference to microchannel and ion blotting microchannel by former water flow direction, described with reference to microchannel outlet and the outlet of ion blotting microchannel all be connected microchannel and connect, connection microchannel exports and is connected with electrophoresis sample introduction microchannel entrance;
Described waste liquid is discharged microchannel and is connected microchannel and connects, and described damping fluid discharge microchannel is connected with electrophoresis sample introduction microchannel;
Be arranged on reference on microchannel with reference to bringing out the micro-valve of mouth, ion blotting exports micro-valve and is arranged on ion blotting microchannel, the micro-valve of waste liquid outlet is arranged on waste liquid and discharges on microchannel, damping fluid ends micro-valve and is arranged on connection microchannel, and be positioned at and connect microchannel, waste liquid is discharged microchannel intersection point and is connected microchannel, the position between the intersection point of microchannel discharged by damping fluid, sample introduction ends micro-valve and is arranged on electrophoresis sample introduction microchannel, and be positioned at electrophoresis sample introduction microchannel, damping fluid discharges microchannel intersection point and electrophoresis sample introduction microchannel, position between the intersection point of electrophoretic separation microchannel.
Detection of heavy metal ion micro-fluidic chip in above-mentioned water, comprises substrate layer, thin layer, micro-valve through holes layer and gas-liquid articulamentum from bottom to top successively;
Described substrate layer is provided with reference to microchannel, ion blotting microchannel, electrophoresis sample introduction microchannel, electrophoretic separation microchannel, connects microchannel, waste liquid discharge microchannel and damping fluid discharge microchannel; Also be provided with multiple iontophoretic electrode, described iontophoretic electrode exports with electrophoresis sample introduction microchannel entrance, electrophoresis sample introduction microchannel respectively, electrophoretic separation microchannel entrance and electrophoretic separation microchannel export and be connected;
Described thin layer is provided with damping fluid inflow entrance, damping fluid liquid storage tank, sample reservoir, sample waste pond, damping fluid waste liquid pool, damping fluid escape hole, sample escape hole and sample channel mouth; Damping fluid inflow entrance is connected the entrance and exit of electrophoretic separation microchannel respectively with damping fluid waste liquid pool, and damping fluid liquid storage tank is connected between electrophoretic separation microchannel entrance and cross electrophoresis point of crossing; Sample reservoir is connected the entrance and exit of electrophoresis sample introduction microchannel respectively with sample waste pond; Damping fluid escape hole connects the outlet that microchannel discharged by damping fluid; Sample escape hole connects the outlet that waste liquid discharges microchannel; Sample channel mouth connects the entrance with reference to microchannel and ion blotting microchannel respectively;
Described micro-valve through holes layer is provided with reference to bringing out the micro-valve of mouth, ion blotting exports micro-valve, the micro-valve of waste liquid outlet, damping fluid end micro-valve and sample introduction ends micro-valve; Also be provided with damping fluid inflow entrance, damping fluid liquid storage tank, sample reservoir, sample waste pond, damping fluid waste liquid pool, damping fluid escape hole, sample escape hole and sample channel mouth;
Described gas-liquid articulamentum is provided with damping fluid inflow entrance, damping fluid liquid storage tank, sample reservoir, sample waste pond, damping fluid waste liquid pool, damping fluid escape hole, sample escape hole and sample channel mouth; Also be provided with many gas passages and gas tunnel holes.
Further, described thin layer comprises one deck electric insulating film.
Further, described micro-valve through holes layer comprises one deck non-deformed flat board.
Detection of heavy metal ion micro-fluidic chip in above-mentioned water, described ion blotting microchannel is many, containing the ion imprinted polymer that can adsorb different heavy metal ion in every bar ion blotting microchannel.
A detection of heavy metal ion method in the water in above water, detection of heavy metal ion micro-fluidic chip realized, after closing all micro-valves, performs following steps successively:
The injection of step a, former water, separation and detection, obtain first electrophoresis pattern of heavy metal ion adsorbed front cross electrophoresis;
The injection of step b, ion blotting adsorption liquid, separation and detection, obtain second electrophoresis pattern of heavy metal ion adsorbed rear cross electrophoresis;
The difference of second electrophoresis pattern that step c, first electrophoresis pattern obtained according to step a and step b obtain, identifies specific heavy metal ion, calculates content of beary metal.
Detection of heavy metal ion method in above-mentioned water, step a is specially:
Step a1, only open sample introduction and end micro-valve, damping fluid is injected electrophoresis sample introduction microchannel and electrophoretic separation microchannel by damping fluid inflow entrance, then closes sample introduction and end micro-valve;
Step a2, open with reference to bringing out the micro-valve of mouth, ion blotting exports micro-valve and the micro-valve of waste liquid outlet, former water is injected with reference to microchannel and ion blotting microchannel by sample channel mouth, unnecessary former water is discharged microchannel by waste liquid and is discharged via sample escape hole, then close ion blotting and export micro-valve and the micro-valve of waste liquid outlet, the ion imprinted polymer in ion blotting microchannel adsorbs the former water rested in ion blotting microchannel;
Step a3, open damping fluid and end micro-valve, former water passes through reference microchannel and is connected microchannel injection in sample reservoir, close damping fluid to end micro-valve and open sample introduction and end micro-valve, under the effect of electrophoresis, obtain first electrophoresis pattern through electrophoretic separation microchannel, close sample introduction and end micro-valve.
Detection of heavy metal ion method in above-mentioned water, step b is specially:
Step b1, only open sample introduction and end micro-valve, damping fluid is injected electrophoresis sample introduction microchannel and electrophoretic separation microchannel by damping fluid inflow entrance, then closes sample introduction and end micro-valve;
Step b2, open with reference to bringing out the micro-valve of mouth and the micro-valve of waste liquid outlet, washed with de-ionized water with reference to microchannel, connect microchannel, discharging microchannel through waste liquid and discharge, closing with reference to bringing out the micro-valve of mouth;
Step b3, open ion blotting and export micro-valve and the micro-valve of waste liquid outlet, the unnecessary sample through ion blotting microchannel discharges microchannel by waste liquid, discharges via sample escape hole, then closes the micro-valve of waste liquid outlet;
Step b4, open damping fluid and end micro-valve, former water through ion blotting microchannel be connected microchannel injection in sample reservoir, close damping fluid to end micro-valve and open sample introduction and end micro-valve, under the effect of electrophoresis, obtain second electrophoresis pattern through electrophoretic separation microchannel, close sample introduction and end micro-valve;
Detection of heavy metal ion method in above-mentioned water, step c is specially: the difference of second electrophoresis pattern that first electrophoresis pattern obtained according to step a and step b obtain, qualitative recognition heavy metal ion kind, or/and quantitatively calculate content of beary metal.
Detection of heavy metal ion method in above water, further comprising the steps of:
Steps d, open ion blotting and export micro-valve and the micro-valve of waste liquid outlet, eluent is injected in ion blotting microchannel, the heavy metal ion of adsorbing in elution ionic trace microchannel, discharges microchannel and the micro-valve of waste liquid outlet is discharged through waste liquid;
Step e, cleaning fluid are injected in ion blotting microchannel, eluent residual in cleaning ion blotting microchannel, discharge microchannel and the micro-valve discharge of waste liquid outlet through waste liquid.
Beneficial effect:
The first, because detection of heavy metal ion equipment of the present invention only realizes on a micro-fluidic chip being integrated with some microchannels and micro-valve, therefore compare with conventional detection devices, have the advantages that volume is little, quality is little, power consumption is little, and then reduce production cost and the use cost of equipment;
The second, because micro-fluidic chip of the present invention is provided with the ion blotting microchannel of Selective recognition ability strong (an adsorbed target heavy metal ion), and coordinate the method for electrophoretic separation to carry out detection of heavy metal ion, even if therefore have multiple heavy metal ion and deposit or have other pollutant to deposit in case, also can not reduce the detection order of accuarcy of micro-fluidic chip, there is very strong antijamming capability;
Three, because the detection of heavy metal ion method that realizes on micro-fluidic chip of the present invention can direct-detection water body, eliminate the preprocessing process of conventional detection devices, therefore testing process is simple, and detection time is short.
Accompanying drawing explanation
Fig. 1 is the structural representation of detection of heavy metal ion micro-fluidic chip specific embodiment one in water of the present invention.
Fig. 2 is the substrate layer structural representation of detection of heavy metal ion micro-fluidic chip specific embodiment two in water of the present invention.
Fig. 3 is the film layer structure schematic diagram of detection of heavy metal ion micro-fluidic chip specific embodiment two in water of the present invention.
Fig. 4 is micro-valve through holes Rotating fields schematic diagram of detection of heavy metal ion micro-fluidic chip specific embodiment two in water of the present invention.
Fig. 5 is the gas-liquid articulamentum structural representation of detection of heavy metal ion micro-fluidic chip specific embodiment two in water of the present invention.
Fig. 6 is the structural representation of detection of heavy metal ion micro-fluidic chip specific embodiment two in water of the present invention.
Fig. 7 is the process flow diagram of detection of heavy metal ion method specific embodiment four in water of the present invention.
Fig. 8 is the process flow diagram of detection of heavy metal ion method step a in water of the present invention.
Fig. 9 is the process flow diagram of detection of heavy metal ion method step b in water of the present invention.
Figure 10 is the process flow diagram of detection of heavy metal ion method specific embodiment eight in water of the present invention.
In figure: 1 substrate layer, 2 thin layers, 3 micro-valve through holes layers, 4 gas-liquid articulamentums, 7 gas passages, 11 with reference to microchannel, 12 ion blotting microchannels, 13 electrophoresis sample introduction microchannels, 14 electrophoretic separation microchannels, 15 connect microchannel, 16 waste liquids discharge microchannel, microchannel discharged by 17 damping fluids, 21 with reference to bringing out the micro-valve of mouth, 22 ion blottings export micro-valve, the micro-valve of 23 waste liquid outlet, 24 damping fluids end micro-valve, 25 sample introductions end micro-valve, 30 iontophoretic electrodes, 80 damping fluid inflow entrances, 81 damping fluid liquid storage tanks, 82 sample reservoir, 83 sample waste ponds, 84 damping fluid waste liquid pools, 85 damping fluid escape holes, 86 sample escape holes, 87 sample channel mouths, 88 gas passage through holes.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the invention is described in further detail.
Specific embodiment one
The present embodiment is detection of heavy metal ion micro-fluidic chip embodiment in water.
Detection of heavy metal ion micro-fluidic chip in the water of the present embodiment, structural representation as shown in Figure 1.This micro-fluidic chip comprises microchannel and micro-valve,
Described microchannel comprises with reference to microchannel 11, ion blotting microchannel 12, electrophoresis sample introduction microchannel 13, electrophoretic separation microchannel 14, connects microchannel 15, waste liquid discharge microchannel 16 and damping fluid discharge microchannel 17;
Described micro-valve comprises with reference to bringing out the micro-valve of mouth 21, ion blotting exports micro-valve 22, the micro-valve of waste liquid outlet 23, damping fluid end micro-valve 24 and sample introduction ends micro-valve 25;
Set gradually electrophoresis sample introduction microchannel 13 and electrophoretic separation microchannel 14 by damping fluid flow direction, described electrophoresis sample introduction microchannel 13 and electrophoretic separation microchannel 14 form cross electrophoresis;
Be set up in parallel with reference to microchannel 11 and ion blotting microchannel 12 by former water flow direction, described export with reference to microchannel 11 and ion blotting microchannel 12 export all be connected microchannel 15 and connect, connection microchannel 15 exports and is connected with electrophoresis sample introduction microchannel 13 entrance;
Described waste liquid is discharged microchannel 16 and is connected microchannel 15 and connects, and described damping fluid discharge microchannel 17 is connected with electrophoresis sample introduction microchannel 13;
Be arranged on reference on microchannel 11 with reference to bringing out the micro-valve 21 of mouth, ion blotting exports micro-valve 22 and is arranged on ion blotting microchannel 12, the micro-valve 23 of waste liquid outlet is arranged on waste liquid and discharges on microchannel 16, damping fluid ends micro-valve 24 and is arranged on connection microchannel 15, and be positioned at and connect microchannel 15, waste liquid is discharged microchannel 16 intersection point and is connected microchannel 15, the position between the intersection point of microchannel 17 discharged by damping fluid, sample introduction ends micro-valve 25 and is arranged on electrophoresis sample introduction microchannel 13, and be positioned at electrophoresis sample introduction microchannel 13, damping fluid discharges microchannel 17 intersection point and electrophoresis sample introduction microchannel 13, position between the intersection point of electrophoretic separation microchannel 14.
Specific embodiment two
The present embodiment is detection of heavy metal ion micro-fluidic chip embodiment in water.
Detection of heavy metal ion micro-fluidic chip in the water of the present embodiment, on the basis of specific embodiment one, limits this chip further, comprises substrate layer 1, thin layer 2, micro-valve through holes layer 3 and gas-liquid articulamentum 4 from bottom to top successively;
As shown in Figure 2, substrate layer 1 is provided with reference to microchannel 11, ion blotting microchannel 12, electrophoresis sample introduction microchannel 13, electrophoretic separation microchannel 14, connects microchannel 15, waste liquid discharge microchannel 16 and damping fluid discharge microchannel 17 structural representation of described substrate layer 1; Also be provided with multiple iontophoretic electrode 30, described iontophoretic electrode 30 exports with electrophoresis sample introduction microchannel 13 entrance, electrophoresis sample introduction microchannel 13 respectively, electrophoretic separation microchannel 14 entrance and electrophoretic separation microchannel 14 export and be connected;
As shown in Figure 3, thin layer 2 is provided with damping fluid inflow entrance 80, damping fluid liquid storage tank 81, sample reservoir 82, sample waste pond 83, damping fluid waste liquid pool 84, damping fluid escape hole 85, sample escape hole 86 and sample channel mouth 87 to the structural representation of described thin layer 2; Damping fluid inflow entrance 80 is connected the entrance and exit of electrophoretic separation microchannel 14 respectively with damping fluid waste liquid pool 84, and damping fluid liquid storage tank 81 is connected between electrophoretic separation microchannel 14 entrance and cross electrophoresis point of crossing; Sample reservoir 82 and sample waste pond 83 are connected the entrance and exit of electrophoresis sample introduction microchannel 13 respectively; Damping fluid escape hole 85 connects the outlet that microchannel 17 discharged by damping fluid; Sample escape hole 86 connects the outlet that waste liquid discharges microchannel 16; Sample channel mouth 87 connects the entrance with reference to microchannel 11 and ion blotting microchannel 12 respectively;
As shown in Figure 4, micro-valve through holes layer 3 is provided with reference to bringing out the micro-valve of mouth 21 structural representation of described micro-valve through holes layer 3, ion blotting exports micro-valve 22, the micro-valve of waste liquid outlet 23, damping fluid end micro-valve 24 and sample introduction ends micro-valve 25; Also be provided with damping fluid inflow entrance 80, damping fluid liquid storage tank 81, sample reservoir 82, sample waste pond 83, damping fluid waste liquid pool 84, damping fluid escape hole 85, sample escape hole 86 and sample channel mouth 87;
As shown in Figure 5, gas-liquid articulamentum 4 is provided with damping fluid inflow entrance 80, damping fluid liquid storage tank 81, sample reservoir 82, sample waste pond 83, damping fluid waste liquid pool 84, damping fluid escape hole 85, sample escape hole 86 and sample channel mouth 87 to the structural representation of described gas-liquid articulamentum 4; Also be provided with many gas passages 7 and gas tunnel holes 88.
Detection of heavy metal ion micro-fluidic chip in the water of the present embodiment, one-piece construction schematic diagram as shown in Figure 6.
In the water of the present embodiment, detection of heavy metal ion micro-fluidic chip, is set to four-layer structure, can realize being completed by chip manufacturing on four layers of pcb board, is conducive to the volume reducing micro-fluidic chip, reduces the cost of manufacture of micro-fluidic chip.
Further, described thin layer 2 comprises one deck electric insulating film.
Further, described micro-valve through holes layer 3 comprises one deck non-deformed flat board.
Present embodiments provide the more specifically implementation of detection of heavy metal ion micro-fluidic chip in a kind of water, but it should be noted that, the realization of this chip, is not limited to four-layer structure.
Specific embodiment three
The present embodiment is detection of heavy metal ion micro-fluidic chip embodiment in water.
Detection of heavy metal ion micro-fluidic chip in the water of the present embodiment, on the basis of above embodiment, limits ion blotting microchannel 12 further and is many, containing the ion imprinted polymer that can adsorb different heavy metal ion in every bar ion blotting microchannel.
Ion blotting microchannel 12 is the technology limiting of many, can realize the imprinted polymer placing the different heavy metal ion of absorption in different ions trace microchannel 12, and then realize the function of an equipment Inspection contents of many kinds of heavy metal ion.
Specific embodiment four
The present embodiment is detection of heavy metal ion embodiment of the method in water.
The present embodiment water in detection of heavy metal ion method, after closing all micro-valves, perform following steps successively:
The injection of step a, former water, separation and detection, obtain first electrophoresis pattern of heavy metal ion adsorbed front cross electrophoresis;
The injection of step b, ion blotting adsorption liquid, separation and detection, obtain second electrophoresis pattern of heavy metal ion adsorbed rear cross electrophoresis;
The difference of second electrophoresis pattern that step c, first electrophoresis pattern obtained according to step a and step b obtain, identifies specific heavy metal ion, calculates content of beary metal.
The method process flow diagram as shown in Figure 7.
Specific embodiment five
The present embodiment is detection of heavy metal ion embodiment of the method in water.
The present embodiment water in detection of heavy metal ion method, on the basis of specific embodiment four, limit further step a and be specially:
Step a1, only open sample introduction and end micro-valve 25, damping fluid is injected electrophoresis sample introduction microchannel 13 and electrophoretic separation microchannel 14 by damping fluid inflow entrance 80, then closes sample introduction and end micro-valve 25;
Step a2, open with reference to bringing out the micro-valve of mouth 21, ion blotting exports micro-valve 22 and the micro-valve 23 of waste liquid outlet, former water is injected with reference to microchannel 11 and ion blotting microchannel 12 by sample channel mouth 87, unnecessary former water is discharged microchannel 16 by waste liquid and is discharged via sample escape hole 86, then close ion blotting and export micro-valve 22 and the micro-valve 23 of waste liquid outlet, the ion imprinted polymer in ion blotting microchannel 12 adsorbs the former water rested in ion blotting microchannel 12;
Step a3, open damping fluid and end micro-valve 24, former water through with reference to microchannel 11 be connected microchannel 15 and be expelled in sample reservoir 82, close damping fluid to end micro-valve 24 and open sample introduction and end micro-valve 25, under the effect of electrophoresis, obtain first electrophoresis pattern through electrophoretic separation microchannel 14, close sample introduction and end micro-valve 25.
The process flow diagram of step a as shown in Figure 8.
Specific embodiment six
The present embodiment is detection of heavy metal ion embodiment of the method in water.
The present embodiment water in detection of heavy metal ion method, on the basis of specific embodiment four, limit further step b and be specially:
Step b1, only open sample introduction and end micro-valve 25, damping fluid is injected electrophoresis sample introduction microchannel 13 and electrophoretic separation microchannel 14 by damping fluid inflow entrance 80, then closes sample introduction and end micro-valve 25;
Step b2, open with reference to bringing out the micro-valve of mouth 21 and the micro-valve 23 of waste liquid outlet, washed with de-ionized water with reference to microchannel 11, connect microchannel 15, discharging microchannel 16 through waste liquid and discharge, closing with reference to bringing out the micro-valve 21 of mouth;
Step b3, open ion blotting and export micro-valve 22 and the micro-valve 23 of waste liquid outlet, the unnecessary sample through ion blotting microchannel 12 discharges microchannel 16 by waste liquid, discharges via sample escape hole 86, then closes the micro-valve 23 of waste liquid outlet;
Step b4, open damping fluid and end micro-valve 24, former water through ion blotting microchannel 12 be connected microchannel 15 and be expelled in sample reservoir 82, close damping fluid to end micro-valve 24 and open sample introduction and end micro-valve 25, under the effect of electrophoresis, obtain second electrophoresis pattern through electrophoretic separation microchannel 14, close sample introduction and end micro-valve 25.
The process flow diagram of step b as shown in Figure 9.
Specific embodiment seven
The present embodiment is detection of heavy metal ion embodiment of the method in water.
The present embodiment water in detection of heavy metal ion method, on the basis of specific embodiment four, further restriction step c is specially: the difference of second electrophoresis pattern that first electrophoresis pattern obtained according to step a and step b obtain, qualitative recognition heavy metal ion kind, or/and quantitatively calculate content of beary metal.
Specific embodiment eight
The present embodiment is detection of heavy metal ion embodiment of the method in water.
The present embodiment water in detection of heavy metal ion method, on the basis of above embodiment of the method, limit further further comprising the steps of:
Steps d, open ion blotting and export micro-valve 22 and the micro-valve 23 of waste liquid outlet, eluent is injected in ion blotting microchannel 12, the heavy metal ion of absorption in elution ionic trace microchannel 12, discharges microchannel 16 and the micro-valve 23 of waste liquid outlet is discharged through waste liquid;
Step e, cleaning fluid are injected in ion blotting microchannel 12, eluent residual in cleaning ion blotting microchannel 12, discharge through waste liquid discharge microchannel 16 and the micro-valve 23 of waste liquid outlet.
The process flow diagram of specific embodiment eight as shown in Figure 10.The increase of steps d and step e, heavy metal ion in ion blotting microchannel 12 can be attached to by wash-out, and wash eluent, avoid the interference that in this testing process, heavy metal ion or other pollutants detected next time, improve the order of accuarcy next time detected.
The present invention is not limited to above-mentioned preferred forms, and anyone should learn the structure change or method improvement made under enlightenment of the present invention, and every have identical or close technical scheme with the present invention, all falls within protection scope of the present invention.
Claims (10)
1. a detection of heavy metal ion micro-fluidic chip in water, is characterized in that, comprises microchannel and micro-valve,
Described microchannel comprises with reference to microchannel (11), ion blotting microchannel (12), electrophoresis sample introduction microchannel (13), electrophoretic separation microchannel (14), connection microchannel (15), waste liquid discharges microchannel (16) and microchannel (17) discharged by damping fluid;
Described micro-valve comprises with reference to bringing out the micro-valve of mouth (21), ion blotting exports micro-valve (22), the micro-valve of waste liquid outlet (23), damping fluid end micro-valve (24) and sample introduction ends micro-valve (25);
Set gradually electrophoresis sample introduction microchannel (13) and electrophoretic separation microchannel (14) by damping fluid flow direction, described electrophoresis sample introduction microchannel (13) and electrophoretic separation microchannel (14) form cross electrophoresis;
Be set up in parallel with reference to microchannel (11) and ion blotting microchannel (12) by former water flow direction, described with reference to microchannel (11) outlet and ion blotting microchannel (12) outlet all be connected microchannel (15) and connect, connection microchannel (15) exports and is connected with electrophoresis sample introduction microchannel (13) entrance;
Described waste liquid is discharged microchannel (16) and is connected microchannel (15) and connects, and described damping fluid discharge microchannel (17) is connected with electrophoresis sample introduction microchannel (13);
Be arranged on reference on microchannel (11) with reference to bringing out the micro-valve of mouth (21), ion blotting exports micro-valve (22) and is arranged on ion blotting microchannel (12), the micro-valve of waste liquid outlet (23) is arranged on waste liquid and discharges on microchannel (16), damping fluid ends micro-valve (24) and is arranged in connection microchannel (15), and be positioned at and connect microchannel (15), waste liquid is discharged microchannel (16) intersection point and is connected microchannel (15), the position between microchannel (17) intersection point discharged by damping fluid, sample introduction ends micro-valve (25) and is arranged on electrophoresis sample introduction microchannel (13), and be positioned at electrophoresis sample introduction microchannel (13), damping fluid discharges microchannel (17) intersection point and electrophoresis sample introduction microchannel (13), position between electrophoretic separation microchannel (14) intersection point.
2. detection of heavy metal ion micro-fluidic chip in water according to claim 1, it is characterized in that, comprise substrate layer (1), thin layer (2), micro-valve through holes layer (3) and gas-liquid articulamentum (4) from bottom to top successively;
Described substrate layer (1) is provided with reference to microchannel (11), ion blotting microchannel (12), electrophoresis sample introduction microchannel (13), electrophoretic separation microchannel (14), connection microchannel (15), waste liquid discharges microchannel (16) and microchannel (17) discharged by damping fluid; Also be provided with multiple iontophoretic electrode (30), described iontophoretic electrode (30) exports with electrophoresis sample introduction microchannel (13) entrance, electrophoresis sample introduction microchannel (13) respectively, electrophoretic separation microchannel (14) entrance and electrophoretic separation microchannel (14) export and be connected;
Described thin layer (2) is provided with damping fluid inflow entrance (80), damping fluid liquid storage tank (81), sample reservoir (82), sample waste pond (83), damping fluid waste liquid pool (84), damping fluid escape hole (85), sample escape hole (86) and sample channel mouth (87); Damping fluid inflow entrance (80) is connected the entrance and exit of electrophoretic separation microchannel (14) respectively with damping fluid waste liquid pool (84), and damping fluid liquid storage tank (81) is connected between electrophoretic separation microchannel (14) entrance and cross electrophoresis point of crossing; Sample reservoir (82) is connected the entrance and exit of electrophoresis sample introduction microchannel (13) respectively with sample waste pond (83); Damping fluid escape hole (85) connects the outlet that microchannel (17) discharged by damping fluid; Sample escape hole (86) connects the outlet that waste liquid discharges microchannel (16); Sample channel mouth (87) connects the entrance with reference to microchannel (11) and ion blotting microchannel (12) respectively;
Described micro-valve through holes layer (3) is provided with reference to bringing out the micro-valve of mouth (21), ion blotting exports micro-valve (22), the micro-valve of waste liquid outlet (23), damping fluid end micro-valve (24) and sample introduction ends micro-valve (25); Also be provided with damping fluid inflow entrance (80), damping fluid liquid storage tank (81), sample reservoir (82), sample waste pond (83), damping fluid waste liquid pool (84), damping fluid escape hole (85), sample escape hole (86) and sample channel mouth (87);
Described gas-liquid articulamentum (4) is provided with damping fluid inflow entrance (80), damping fluid liquid storage tank (81), sample reservoir (82), sample waste pond (83), damping fluid waste liquid pool (84), damping fluid escape hole (85), sample escape hole (86) and sample channel mouth (87); Also be provided with many gas passages (7) and gas tunnel holes (88).
3. detection of heavy metal ion micro-fluidic chip in water according to claim 2, is characterized in that, described thin layer (2) comprises one deck electric insulating film.
4. detection of heavy metal ion micro-fluidic chip in water according to claim 2, is characterized in that, it is dull and stereotyped that described micro-valve through holes layer (3) comprises one deck non-deformed.
5. detection of heavy metal ion micro-fluidic chip in the water according to claim 1,2,3 or 4, it is characterized in that, described ion blotting microchannel (12) is many, containing the ion imprinted polymer that can adsorb different heavy metal ion in every bar ion blotting microchannel.
6. a detection of heavy metal ion method in the water in water described in claim 1,2,3,4 or 5, detection of heavy metal ion micro-fluidic chip realized, is characterized in that, after closing all micro-valves, performs following steps successively:
The injection of step a, former water, separation and detection, obtain first electrophoresis pattern of heavy metal ion adsorbed front cross electrophoresis;
The injection of step b, ion blotting adsorption liquid, separation and detection, obtain second electrophoresis pattern of heavy metal ion adsorbed rear cross electrophoresis;
The difference of second electrophoresis pattern that step c, first electrophoresis pattern obtained according to step a and step b obtain, identifies specific heavy metal ion, calculates content of beary metal.
7. detection of heavy metal ion method in water according to claim 6, it is characterized in that, step a is specially:
Step a1, only open sample introduction and end micro-valve (25), damping fluid is injected electrophoresis sample introduction microchannel (13) and electrophoretic separation microchannel (14) by damping fluid inflow entrance (80), then closes sample introduction and end micro-valve (25);
Step a2, open with reference to bringing out the micro-valve of mouth (21), ion blotting exports micro-valve (22) and the micro-valve of waste liquid outlet (23), former water is injected with reference to microchannel (11) and ion blotting microchannel (12) by sample channel mouth (87), unnecessary former water is discharged microchannel (16) by waste liquid and is discharged via sample escape hole (86), then close ion blotting and export micro-valve (22) and the micro-valve of waste liquid outlet (23), ion imprinted polymer in ion blotting microchannel (12) adsorbs the former water rested in ion blotting microchannel (12),
Step a3, open damping fluid and end micro-valve (24), former water through with reference to microchannel (11) be connected microchannel (15) and be expelled in sample reservoir (82), close damping fluid to end micro-valve (24) and open sample introduction and end micro-valve (25), under the effect of electrophoresis, obtain first electrophoresis pattern through electrophoretic separation microchannel (14), close sample introduction and end micro-valve (25).
8. detection of heavy metal ion method in water according to claim 6, it is characterized in that, step b is specially:
Step b1, only open sample introduction and end micro-valve (25), damping fluid is injected electrophoresis sample introduction microchannel (13) and electrophoretic separation microchannel (14) by damping fluid inflow entrance (80), then closes sample introduction and end micro-valve (25);
Step b2, open with reference to bringing out the micro-valve of mouth (21) and the micro-valve of waste liquid outlet (23), washed with de-ionized water is with reference to microchannel (11), connection microchannel (15), discharging microchannel (16) through waste liquid to discharge, closing with reference to bringing out the micro-valve of mouth (21);
Step b3, open ion blotting and export micro-valve (22) and the micro-valve of waste liquid outlet (23), the unnecessary sample through ion blotting microchannel (12) discharges microchannel (16) by waste liquid, discharge via sample escape hole (86), then close the micro-valve of waste liquid outlet (23);
Step b4, open damping fluid and end micro-valve (24), former water through ion blotting microchannel (12) be connected microchannel (15) and be expelled in sample reservoir (82), close damping fluid to end micro-valve (24) and open sample introduction and end micro-valve (25), under the effect of electrophoresis, obtain second electrophoresis pattern through electrophoretic separation microchannel (14), close sample introduction and end micro-valve (25).
9. detection of heavy metal ion method in water according to claim 6, it is characterized in that, step c is specially: the difference of second electrophoresis pattern that first electrophoresis pattern obtained according to step a and step b obtain, qualitative recognition heavy metal ion kind, or/and quantitatively calculate content of beary metal.
10. detection of heavy metal ion method in the water according to claim 6,7,8 or 9, is characterized in that, further comprising the steps of:
Steps d, open ion blotting and export micro-valve (22) and the micro-valve of waste liquid outlet (23), eluent is injected in ion blotting microchannel (12), the heavy metal ion of absorption in elution ionic trace microchannel (12), discharges microchannel (16) through waste liquid and the micro-valve of waste liquid outlet (23) is discharged;
Step e, cleaning fluid are injected in ion blotting microchannel (12), eluent residual in cleaning ion blotting microchannel (12), discharge microchannel (16) and the micro-valve of waste liquid outlet (23) discharge through waste liquid.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108279230A (en) * | 2018-02-02 | 2018-07-13 | 哈尔滨工业大学 | A kind of micro-fluidic type water quality toxicity analysis and detection device and its determination method |
| CN108760661A (en) * | 2018-09-08 | 2018-11-06 | 重庆科技学院 | A kind of petroleum wastewater heavy metal ion multi-channel detection chip |
| CN108872081A (en) * | 2018-09-04 | 2018-11-23 | 重庆科技学院 | A kind of multi-layer micro-fluidic chips detecting heavy metal ion |
| CN109142299A (en) * | 2018-09-08 | 2019-01-04 | 重庆科技学院 | A kind of petroleum wastewater heavy metal ion detection chip application method |
| CN112899140A (en) * | 2021-01-21 | 2021-06-04 | 中国科学技术大学 | Micro-fluidic chip for multi-parameter detection of water body |
| CN114088789A (en) * | 2021-12-10 | 2022-02-25 | 大连海事大学 | Drug detection device and method based on micro-fluidic control |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1991356A (en) * | 2005-12-31 | 2007-07-04 | 博奥生物有限公司 | Multiple-pass capillary tube electrophoresis chip and voltage control method thereof |
| CN103033596A (en) * | 2012-11-23 | 2013-04-10 | 南昌大学 | Application of magnetic molecular imprinting technique in chiral recognition of microfluidic system |
-
2015
- 2015-12-15 CN CN201510929066.3A patent/CN105424784A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1991356A (en) * | 2005-12-31 | 2007-07-04 | 博奥生物有限公司 | Multiple-pass capillary tube electrophoresis chip and voltage control method thereof |
| CN103033596A (en) * | 2012-11-23 | 2013-04-10 | 南昌大学 | Application of magnetic molecular imprinting technique in chiral recognition of microfluidic system |
Non-Patent Citations (5)
| Title |
|---|
| GERALD M.BIRNBAUMER 等: "Detection of viruses with molecularly imprinted polymers integrated on a microfluidic biochip using contact-less dielectric microsensors", 《LAB ON A CHIP》 * |
| YASUMOTO DATE 等: "Trace-Level Mercury Ion (Hg2+) Analysis in Aqueous Sample Based on Solid-Phase Extraction Followed by Microfluidic Immunoassay", 《ANALYTICAL CHEMISTRY》 * |
| 李江梅: "微流控芯片上分子印迹固相萃取装置的构建及其应用", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
| 胡娜娜 等: "微流控芯片中金属离子印迹整体柱集成及用于Cd2+富集和在线检测", 《分析科学学报》 * |
| 赵林: "多层复合结构微流控芯片关键技术研究", 《中国优秀硕士学位论文全文数据库 信息科技辑》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108279230A (en) * | 2018-02-02 | 2018-07-13 | 哈尔滨工业大学 | A kind of micro-fluidic type water quality toxicity analysis and detection device and its determination method |
| CN108279230B (en) * | 2018-02-02 | 2020-06-09 | 哈尔滨工业大学 | Micro-flow control type water quality toxicity analysis and detection device and detection and analysis method thereof |
| CN108872081A (en) * | 2018-09-04 | 2018-11-23 | 重庆科技学院 | A kind of multi-layer micro-fluidic chips detecting heavy metal ion |
| CN108872081B (en) * | 2018-09-04 | 2023-06-23 | 重庆科技学院 | Multilayer microfluidic chip for detecting heavy metal ions |
| CN108760661A (en) * | 2018-09-08 | 2018-11-06 | 重庆科技学院 | A kind of petroleum wastewater heavy metal ion multi-channel detection chip |
| CN109142299A (en) * | 2018-09-08 | 2019-01-04 | 重庆科技学院 | A kind of petroleum wastewater heavy metal ion detection chip application method |
| CN108760661B (en) * | 2018-09-08 | 2023-06-09 | 重庆科技学院 | Multi-channel detection chip for heavy metal ions in petroleum wastewater |
| CN109142299B (en) * | 2018-09-08 | 2023-07-18 | 重庆科技学院 | Application method of petroleum wastewater heavy metal ion detection chip |
| CN112899140A (en) * | 2021-01-21 | 2021-06-04 | 中国科学技术大学 | Micro-fluidic chip for multi-parameter detection of water body |
| CN114088789A (en) * | 2021-12-10 | 2022-02-25 | 大连海事大学 | Drug detection device and method based on micro-fluidic control |
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