CN105424427A - Microscope glass slide - Google Patents
Microscope glass slide Download PDFInfo
- Publication number
- CN105424427A CN105424427A CN201410480530.0A CN201410480530A CN105424427A CN 105424427 A CN105424427 A CN 105424427A CN 201410480530 A CN201410480530 A CN 201410480530A CN 105424427 A CN105424427 A CN 105424427A
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- CN
- China
- Prior art keywords
- cell
- cells
- glass slide
- microslide
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The invention discloses a novel microscope glass slide. By the utilization of the microscope glass slide, liquid-based thin-layer cells are not damaged, and a large quantity of cells can be transferred to the glass slide. 3% of methyl alcohol is contained in liquid-based cell preserving fluid, and alcohol ions (CHO-1) and hydrogen ions (H+) are attached to the surfaces of cells stored in the liquid-based cell preserving fluid. Through electrochemical treatment, a ferric trichloride (Fe (OH) 3) coating is formed on the surface of the glass slide. In the process of cell transferring, because the alcohol ions (CHO-) and the hydrogen ions (H+) are contained in the cells, the cells are quite easy to adsorb by Fe (OH) 3 on the glass slide, and then a large quantity of cells can be smoothly transferred to the glass slide from a filtering membrane. Meanwhile, the problems that cell transferring is insufficient, and cells are prone to deform are solved to the utmost extent, and the purposes of making a high-quality slide and achieving accurate diagnosis are achieved.
Description
Technical field
The present invention relates to a kind of microslide used in clinical pathology inspection, in particular for the microslide of Thinprep pap test.
Background technology
Microslide is used for putting glass sheet or the piezoid of thing when being microscopic examination thing.When making sample, cell or tissue section is placed on microslide, cover glass is placed on it, be used as to observe.It is an important step of film-making that cell is transferred on microslide, and cell or tissue can effectively be transferred on slide, is the key of slice-making quality quality.
Conventional microslide is the mode by contact, the cell on filtering membrane is made to transfer on microslide, but this transfer has the problem in practical application, the cell namely on filtering membrane can not effectively be transferred on microslide, causes incomplete, a large amount of cell of transfer to be lost.Also the problem adopting the way heated microslide to be lost to process incomplete, a large amount of cell of cell transfer is had, namely on microslide geometrical clamp, a heating arrangement is loaded onto, heat transfer is passed through after microslide puts up, microslide is heated, when cell shifts, the contact due to cold and hot object makes cell transfer to microslide to get on.But the serious problem that this method also exists, mainly cell temperature distortion, causes cell, born of the same parents' oar, karyon temperature distortion, sustains damage, bring problem, cause difficult diagnosis to the diagnosis of medical science, easily occur mistaken diagnosis, mistake examines.
Summary of the invention
The invention provides a kind of Novel microscope microslide, the Thinprep pap test that can make to be filtered film absorption is injury-free, and the larger microslide of transferring to of quantity gets on.
Technical scheme of the present invention is such: a kind of Novel microscope microslide, processes conventional slide with chemical reagent, makes it with electrostatic.Be stored in the cell in liquid based cell preservative fluid, its surface is with alcohol radical ion (CHO
-) and hydrogen ion (H
+).When cell shifts, because cell is with alcohol radical ion (CHO
-) and hydrogen ion (H
+), to be easy to adsorb by the electrostatic on microslide, thus realize a large amount of cells smoothly from filtering membrane again to the process of slide transfer.
The invention has the beneficial effects as follows, cell can the microslide of transferring to of huge amount get on, and cell can not be out of shape, and can reach high-quality film-making, the object of Precise Diagnosis.
Specific embodiments
Microslide of the present invention to achieve the goal effect by adopting chemically treated method to conventional slide.Carry out galvanochemistry process in early stage to conventional slide, namely first soak slide with chemical reagent, have a certain amount of ferric trichloride in reagent mixed liquor, microslide soaks in mixed liquor, microslide will cover be coated with to contain Fe (OH)
3the coating of composition, it is a kind of electrolyte, and this colloid makes microslide produces electrostatic, has the function of adion.The air-dry packaging of microslide, in use owing to touching liquid, it just has adsorption function to cell contained in liquid.In liquid based cell preservative fluid containing 3% methyl alcohol, while preservation effect is played to cell, exist in conserving liquid alcohol radical ion (CHO
-) and hydrogen ion (H
+), after cell enters conserving liquid, through fully stirring, make cell surface have alcohol radical ion (CHO
-) and hydrogen ion (H
+).When film-making is shifted, more easily by the Fe (OH) on microslide
3adsorbed, thus realized cell smoothly from filtering membrane again to the process of microslide transfer.Prevent cell from shifting insufficient or sustaining damage, reach high-quality film-making, the object of Precise Diagnosis.
Claims (2)
1. a microslide, transfers on microslide by the cell be attached on filtering membrane by the mode of directly contact, it is characterized in that on microslide with ferric trichloride (Fe (OH)
3) coating, be stored in cell in cell-preservation liquid with alcohol radical ion (CHO
-) and hydrogen ion (H
+).
2. a kind of microslide according to claim 1, is characterized in that the methyl alcohol containing 3% in cell-preservation liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410480530.0A CN105424427A (en) | 2014-09-19 | 2014-09-19 | Microscope glass slide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410480530.0A CN105424427A (en) | 2014-09-19 | 2014-09-19 | Microscope glass slide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105424427A true CN105424427A (en) | 2016-03-23 |
Family
ID=55502781
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410480530.0A Pending CN105424427A (en) | 2014-09-19 | 2014-09-19 | Microscope glass slide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105424427A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101486532A (en) * | 2008-01-18 | 2009-07-22 | 中山大学达安基因股份有限公司 | Biological chip aldehyde glass carrier |
| CN101556227A (en) * | 2008-04-10 | 2009-10-14 | 孝感宏翔生物医械技术有限公司 | Method for transiting cells through electrostatic adherence |
| JP2012068669A (en) * | 2002-04-26 | 2012-04-05 | Ventana Medical Syst Inc | Method and apparatus for automated coverslipping |
-
2014
- 2014-09-19 CN CN201410480530.0A patent/CN105424427A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012068669A (en) * | 2002-04-26 | 2012-04-05 | Ventana Medical Syst Inc | Method and apparatus for automated coverslipping |
| CN101486532A (en) * | 2008-01-18 | 2009-07-22 | 中山大学达安基因股份有限公司 | Biological chip aldehyde glass carrier |
| CN101556227A (en) * | 2008-04-10 | 2009-10-14 | 孝感宏翔生物医械技术有限公司 | Method for transiting cells through electrostatic adherence |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160323 |
|
| WD01 | Invention patent application deemed withdrawn after publication |