CN101486532A - Biological chip aldehyde glass carrier - Google Patents
Biological chip aldehyde glass carrier Download PDFInfo
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- CN101486532A CN101486532A CNA2008100258662A CN200810025866A CN101486532A CN 101486532 A CN101486532 A CN 101486532A CN A2008100258662 A CNA2008100258662 A CN A2008100258662A CN 200810025866 A CN200810025866 A CN 200810025866A CN 101486532 A CN101486532 A CN 101486532A
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Abstract
The invention relates to a biochip glass slide, in particular to a biochip glass slide prepared by employing an aldehyde group method. The surface of the glass slide is decorated by aldehyde group, thus being convenient for the chemical combination between a nucleic acid probe decorated by amino-group, the antigen-antibody of protein property and other matters. The invention is suitable for the probes of nucleic acids, proteins, and other types, as well as template segment point system microarray chips, has firm and specific chemical bond combining characteristics, and provides the reliable carrier for preparing biochips.
Description
Affiliated technical field
The present invention relates to a kind of biochip carrier slide, specially refer to a kind of biological chip glass carrier that adopts the aldehyde group method preparation.The Chemical bond of amido modified nucleic acid probe and proteinaceous antigen-antibody and other material is convenient in the modification of slide surface aldehyde radical.The present invention is suitable for probe, the template fragment point system micro-array chip of nucleic acid class, protein-based and other kind, has firm special chemical bond in conjunction with characteristics, for the preparation of biochip provides reliable carrier.
Background technology
Biochip is applied to bio-science field more and more with the detecting pattern of its high-throughput, many target position.With the slide glass is the micro-array chip of carrier, and characteristics such as more efficient with it, high-throughput, low price have been widely used in fundamental research and clinical examination, auxiliary diagnosis.
Micro-array chip comprises oligonucleotide chip, cDNA chip, antigen chip, antibody chip etc., with the slide glass after the special chemical process processing is carrier, various probes or target fragment are fixed on surface of glass slide in the mode of chemical bond combination, form the micro-array chip that can be used for hybridization or antigen antibody reaction.
As the slide glass of micro-array chip carrier, the surface chemical modification method is a lot, comprises amination, aldehyde radicalization, sulfhydrylation, epoxy ethylization etc., can reach the chemically combined purpose of target fragment and surface of glass slide.But, for above any surface chemical modification method, the technology of preparing of every family and technology all have difference, and that all is that all right is ripe for overall technological method, the slide glass of preparation exists that background is too high, the uniform surface degree is not enough, bonding force is not enough, hybridize respectively problems such as rate is bad, point of sample irregularity, influence the subsequent preparation and the oeverall quality of micro-array chip, hindered the development of micro-array chip technology.
Summary of the invention
The purpose of this invention is to provide a kind of aldehyde glass carrier with special technique and prepared, this slide glass background is low, uniform surface, bonding force are strong, hybridize that rate height, point of sample size and shape are regular respectively.Can be widely used in the preparation of high-quality oligonucleotide chip, cDNA chip, antigen chip, antibody chip etc., thereby solve the problems referred to above well.
Micro-array chip aldehyde glass carrier provided by the invention, preparation procedure is as follows:
1, blank slide treatment process: the vitriol oil-potassium bichromate solution soaked 8 hours, washed 5 times; The ammoniacal liquor washing lotion (ammoniacal liquor: hydrogen peroxide: water=1:1:5) soaked 8 hours, wash 5 times; Put into distilled water, supersound washing 5 minutes; 100 ℃ were toasted 45 minutes.
2, amination treatment process: blank was put into aminosilane reaction solution (95% ethanol 600ml, Glacial acetic acid 600ul, aminosilane 12ml) 25 minutes, 95% ethanol supersound washing 5 minutes, distilled water supersound washing 5 minutes, 100 ℃ of oven dry in 45 minutes.
3, aldehyde radical sheet preparation technology: amino sheet was put into aldehyde radical reaction solution (25% glutaraldehyde 260ml+ distilled water 390ml) ultrasonic 5 minutes, non-again ultrasonic reaction 40 minutes, and distilled water supersound washing 3 times 5 minutes/each, is taken out drying at room temperature.
By above special preparation technology, make aldehyde glass carrier of the present invention, place a week after, can be used for point sample and prepare micro-array chip.
Description of drawings
The micro-array chip synoptic diagram that Fig. 1 point sample is prepared into
1 aldehyde glass carrier
The microarray of 2 point samples preparation
Fig. 2 is hybridized the back and is shown positive probe points
1 positive probe points
2 negative probe points
Embodiment
The present invention makes an explanation with the following example, and purpose is just in order to explain rather than limit by any way the present invention.
Embodiment 1: the aldehyde glass carrier point sample prepares micro-array chip
As shown in Figure 1, get one of aldehyde glass carrier of the present invention, stick and separate fence, be equipped with HLA-DR4 gene type oligonucleotide probe (17), and the sampling liquid composition as hybridization region, by point sample instrument with probe points on slide.The probe matrix is that each probe laterally repeats 3 points, 9 some delegation, totally 6 row.The probe points size of aldehyde radical slide point system of the present invention is even, and shape is regular.
Embodiment 2: by the characteristics of signals of hybridization check micro-array chip
The chip placement that above-mentioned point makes is spent the night, respectively washed 1 minute, dry with 2 * SSC and distilled water.Get other composition of HLA-DR4 gene type reagent, the dna sample of 1 part of known type of amplification obtains hybridizing template.Get PCR product 2ul and be added to the chip hybridization district, (5 * SSC) 8ul, chip are put in the groove of automatic hybridization washing instrument, set 52 ℃ of hybridization temperatures, hybridization time 40 minutes to add hybridization solution again.The washing and air-dry automatically of hybridization back.
Adopt the GnenPix4100A scanner, the scanning hybridization signal obtains results of hybridization scintigram (as shown in Figure 2), and image transitions is become data, adopts analysis software, and above data-switching is become the gene type.Positive hybridization point and negative hybridization point that interpretation of result obtains meet with sample gene order and gene type, as seen from the figure, positive probe points signal is strong, and negative some background is low, bonding force and resolving power are all fine, can satisfy the preparation and the hybridization requirement of micro-array chip.
Claims (6)
1, a kind of biological chip aldehyde glass carrier is characterized in that aldehyde glass carrier is to be prepared from by blank slide treatment process, amination treatment process and aldehyde radical sheet preparation technology.
2, biological chip aldehyde glass carrier according to claim 1, its feature are that also blank slide treatment process is:
The vitriol oil-potassium bichromate solution soaked 8 hours, washed 5 times; The ammoniacal liquor washing lotion (ammoniacal liquor: hydrogen peroxide: water=1:1:5) soaked 8 hours, wash 5 times; Put into distilled water, supersound washing 5 minutes; 100 ℃ were toasted 45 minutes.
3, biological chip aldehyde glass carrier according to claim 1, its feature are that also the amination treatment process is:
Blank was put into aminosilane reaction solution (95% ethanol 600ml, Glacial acetic acid 600ul, aminosilane 12ml) 25 minutes, 95% ethanol supersound washing 5 minutes, distilled water supersound washing 5 minutes, 100 ℃ of oven dry in 45 minutes.
4, biological chip aldehyde glass carrier according to claim 1, its feature are that also aldehyde radical sheet preparation technology is:
Amino sheet was put into aldehyde radical reaction solution (25% glutaraldehyde 260ml+ distilled water 390ml) ultrasonic 5 minutes, non-again ultrasonic reaction 40 minutes, and distilled water supersound washing 3 times 5 minutes/each, is taken out drying at room temperature.
5, biological chip aldehyde glass carrier according to claim 1, its feature also be aldehyde glass carrier can with probes such as oligonucleotide, cDNA, antigen, antibody and target with chemical bond covalency mortise.
6, biological chip aldehyde glass carrier according to claim 1 is characterized in that aldehyde glass carrier can be widely used in the preparation of high-quality oligonucleotide chip, cDNA chip, antigen chip, antibody chip etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100258662A CN101486532B (en) | 2008-01-18 | 2008-01-18 | Biological chip aldehyde glass carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100258662A CN101486532B (en) | 2008-01-18 | 2008-01-18 | Biological chip aldehyde glass carrier |
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| Publication Number | Publication Date |
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| CN101486532A true CN101486532A (en) | 2009-07-22 |
| CN101486532B CN101486532B (en) | 2011-12-21 |
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| CN2008100258662A Active CN101486532B (en) | 2008-01-18 | 2008-01-18 | Biological chip aldehyde glass carrier |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103197071A (en) * | 2013-03-22 | 2013-07-10 | 广州兹尼生物科技有限公司 | Preparation and application of human apoptosis factor quantitative antibody chip |
| CN103412128A (en) * | 2013-08-30 | 2013-11-27 | 华中农业大学 | Method for detecting toxin T-2 in viscera of animals by using immune chip |
| CN103487589A (en) * | 2013-10-16 | 2014-01-01 | 深圳市大爱医疗科技有限公司 | Protein chip kit marked by quantum dot and preparation method thereof |
| CN103755153A (en) * | 2014-01-22 | 2014-04-30 | 南通天盛新能源科技有限公司 | Method for preparing substrate for high formyl group functional group density biological chip |
| CN105424427A (en) * | 2014-09-19 | 2016-03-23 | 孝感宏翔生物医械技术有限公司 | Microscope glass slide |
| CN105510596A (en) * | 2015-12-02 | 2016-04-20 | 深圳市赛尔生物技术有限公司 | Protein chip for detecting respiratory virus antigens as well as kit and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1920053A (en) * | 2005-08-23 | 2007-02-28 | 河南省生物工程技术研究中心 | Autoluminescence fluorescence probe gene chip |
-
2008
- 2008-01-18 CN CN2008100258662A patent/CN101486532B/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103197071A (en) * | 2013-03-22 | 2013-07-10 | 广州兹尼生物科技有限公司 | Preparation and application of human apoptosis factor quantitative antibody chip |
| CN103412128A (en) * | 2013-08-30 | 2013-11-27 | 华中农业大学 | Method for detecting toxin T-2 in viscera of animals by using immune chip |
| CN103412128B (en) * | 2013-08-30 | 2015-07-15 | 华中农业大学 | Method for detecting toxin T-2 in viscera of animals by using immune chip |
| CN103487589A (en) * | 2013-10-16 | 2014-01-01 | 深圳市大爱医疗科技有限公司 | Protein chip kit marked by quantum dot and preparation method thereof |
| CN103755153A (en) * | 2014-01-22 | 2014-04-30 | 南通天盛新能源科技有限公司 | Method for preparing substrate for high formyl group functional group density biological chip |
| CN103755153B (en) * | 2014-01-22 | 2016-03-30 | 南通天盛实验器材有限公司 | A kind of method preparing high aldehyde functions's density biochip substrate |
| CN105424427A (en) * | 2014-09-19 | 2016-03-23 | 孝感宏翔生物医械技术有限公司 | Microscope glass slide |
| CN105510596A (en) * | 2015-12-02 | 2016-04-20 | 深圳市赛尔生物技术有限公司 | Protein chip for detecting respiratory virus antigens as well as kit and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN101486532B (en) | 2011-12-21 |
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Owner name: GUANGZHOU DARUI ANTIBODY ENGINEERING TECHNOLOGY CO Free format text: FORMER OWNER: DAAN GENE CO., LTD., ZHONGSHAN UNIV. Effective date: 20110721 Free format text: FORMER OWNER: GUANGZHOU DATAI BIOENGINEERING TECHNOLOGY CO., LTD. |
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Effective date of registration: 20110721 Address after: 510665 No. 19 incense Hill Road, science and Technology Town, Guangzhou hi tech Development Zone, Guangdong, China Applicant after: Guangzhou Darui Antibodies Engineering Technology Co.Ltd Address before: 510665 No. 19 incense Hill Road, science and Technology Town, Guangzhou hi tech Development Zone, Guangdong, China Applicant before: Daan Gene Co., Ltd., Zhongshan Univ. Co-applicant before: Guangzhou Datai Bioengineering Technology Co., Ltd. |
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Address after: 510665 No. 19 incense Hill Road, science and Technology Town, Guangzhou hi tech Development Zone, Guangdong, China Patentee after: Guangzhou Da Rui Biotechnology Ltd. Address before: 510665 No. 19 incense Hill Road, science and Technology Town, Guangzhou hi tech Development Zone, Guangdong, China Patentee before: Guangzhou Darui Antibodies Engineering Technology Co.Ltd |