CN105424415A - Online automatic collection and storage device for aquatic organism samples - Google Patents

Online automatic collection and storage device for aquatic organism samples Download PDF

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Publication number
CN105424415A
CN105424415A CN201510766516.1A CN201510766516A CN105424415A CN 105424415 A CN105424415 A CN 105424415A CN 201510766516 A CN201510766516 A CN 201510766516A CN 105424415 A CN105424415 A CN 105424415A
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sample
peristaltic pump
sampling
tube
collection
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CN105424415B (en
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张效伟
刘萌斐
杨江华
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Nanjing University
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Nanjing University
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14Suction devices, e.g. pumps; Ejector devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36Embedding or analogous mounting of samples
    • GPHYSICS
    • G05CONTROLLING; REGULATING
    • G05BCONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
    • G05B19/00Programme-control systems
    • G05B19/02Programme-control systems electric
    • G05B19/04Programme control other than numerical control, i.e. in sequence controllers or logic controllers
    • G05B19/05Programmable logic controllers, e.g. simulating logic interconnections of signals according to ladder diagrams or function charts

Abstract

The invention discloses an online automatic collection and storage device for aquatic organism samples. The device is composed of a control unit, a support, a sample feeding unit, a distribution unit and a collection unit. The online automatic collection and storage device is programmed through a PLC and can operate all actions automatically according to preset parameters, accurate sampling is achieved, the automation degree is high, sampling efficiency is high, and manpower and material resource cost is saved; a depth sensor is adopted for the device, accurate control over the sampling depth is achieved, layered sampling can be conducted on water bodies at different depths, and the water sample detection environment is made to more approach the natural environment; running time of a peristaltic pump is controlled in a quantitative mode, accurate control over the filtered water yield is achieved, and the problem that in most of existing technologies, the filtered water yield is large in error is solved; by means of the device, the samples can be collected automatically, the function of adding a fixing agent in a quantitative mode at site is achieved, and stability of the collected samples is improved.

Description

A kind of on-line automatic collection of hydrobiont sample and save set
Technical field
The present invention relates to the on-line automatic collection of a kind of hydrobiont sample and save set, this device automatically can gather in position and preserve the planktonic organism sample of different depth.
Background technology
When carrying out environmental health diagnosis to water bodys such as river, lake, oceans, need to carry out sampling survey to hydroplankton sample, understand planktonic diversity situation.In current aquatic ecosystem, planktonic organism sample to drag netting gear or chartering back and forth and drags netting gear mainly through artificial and carry out sample collection along bank, the method has following open defect: (1) manual operation, inefficiency: large by constraints such as water body bank, ships, sample range is limited, and sample representativeness is poor; And the human and material resources that cost a lot of money, inefficiency; (2) sampling depth is difficult to accurate control: by water currents, very easily floats and forms very large trawlnet angle from sampling spot, cause the change of sampling depth during plankton net sampling; (3) error of calculation of drainage amount is larger: due to the change of plankton net sampling depth, there is comparatively big error according to the drainage amount that wire rope release length, sampling angle and sampling time estimate, and the phytoplankton biological density calculated with this drainage gauge and actual conditions are not inconsistent; (4) only possess acquisition function, can not sample be preserved: during actual samples, sampling thief is often just on sample collection, does not possess hold function.
In order to solve the problem, improve the accuracy of sampling, many researchers have all carried out studying a series ofly and exploring.As Authorization Notice No. CN202057508U introduces pulley support frame, water sampling device and filtering biological sampling thief are coupled together and collects phytoplankton sample, quantitative collection can be carried out to the sample of different water depth, sampling efficiency is improve largely, but package unit also needs by manual operation, especially when sampling frequent, maintenance cost is larger.Authorization Notice No. CN203181818U devises the drive motor that can swim in movement on the water surface, installs the motor steering device that can change working direction at its leading portion, and tail end is installed and collected planktonic netting gear, by the collected specimens that moves around of netting gear; This device can realize automatic sampling, but is difficult to accurate control to sampling depth and drainage amount.The water inlet of corresponding planktonic organism screen pack received by submersible pump by patent publication No. CN101487772A respectively by multichannel shunt, accurately sample size and sampling depth is controlled by Single-chip Controlling solenoid valve, electronic flow-meter, pressure controller etc., automaticity is high, but this device can not realize the Sample storage of original position, do not possess timing sampling function, fail to realize the sampling to same point position different time sections yet.
Summary of the invention
1, the technical matters that will solve is invented
For above-mentioned Problems existing, the object of the invention is to be to provide a kind of novel on-line automatic collection of hydrobiont sample and save set, this device can with buoy dump coupling, realize the sampling at regular time and quantity to different water levels and preservation.
2. technical scheme
For reaching this object, the technical solution adopted in the present invention is:
A kind of on-line automatic collection of hydrobiont sample and save set, this device is made up of control module, support, sample injection unit, allocation units and collector unit;
Described control module comprises main control module, power supply and man-machine interface, and described main control module is connected with described man-machine interface, by Power supply by cable; Main control module is the PLC of self-programming, is connected with stepper motor, depth transducer, optoelectronic switch, solenoid valve by cable; Power supply is semibridge system variable-voltage power supply, and input voltage is AC110v ~ 220v, and output voltage is DC5v, 12v and 24v; Man-machine interface selects graphic control panel, can automatically sample, manual sampling action and parameter setting operation; Programmed by PLC, can automatically run everything according to parameter preset and realize accurately sampling, automaticity is high, and sampling efficiency is fast, and saves human and material resources cost.
Described support is little by density, and the resistant material that intensity is high is made, and comprises framework, back up pad, base plate, upper plate and column, and described framework is cavity body structure main frame, is provided with control module mounting hole and man-machine interface mounting hole; Back up pad is installed in framework; Base plate is fixed on framework, and center is provided with rotating shaft passing hole; Described upper plate is provided with circumference uniform distribution collection tube and round determines hole, is connected as a single entity by column and base plate.
Described sample injection unit comprises depth transducer, stopple coupon, drum, stepper motor, peristaltic pump I, peristaltic pump II, flange bracket and fixing agent, wherein drum is the carrier of depth transducer connecting line, described stopple coupon tube head part and depth transducer synchronization lifting; Connected by flange bracket between described drum and stepper motor and be fixed in framework; Described peristaltic pump I is all connected with main control module with peristaltic pump II; Described peristaltic pump I is connected with water sample, and peristaltic pump II is connected with fixing agent; Employing depth transducer achieves the accurate control to sampling depth, can carry out stratified sampling to the water body of different depth, makes the environment detecting water sample closer to physical environment;
The depth transducer of sample injection unit adopts throw-in type liquid transmitter, powered by power supply, the entrance pressure hole of the other end can fully contact with water body and the data measured are sent to main control module by sensor, the rotation of drum is driven to carry out retractable cable according to sampling depth setting by stepper motor, till sampling set depth is consistent with the actual depth of water; Described peristaltic pump I is all connected with main control module with peristaltic pump II, by the rotation of input pulse or time controling peristaltic pump with stop interval determining the sampling volume of water sample and fixing agent; Described peristaltic pump I is water inlet sample pump; Described peristaltic pump II is for entering fixing agent pump; By the duration of runs of fixing quantity peristaltic pump, realize the accurate control to drainage amount, overcome the problem that existing most of technology drainage amount error is larger;
Described allocation units comprise stepper motor II, flange bracket II, small pulley, Timing Belt, large belt wheel, rotating shaft, snap ring, lower bearing, step, head bearing, top chock, register pin, optoelectronic switch, axle sleeve, sample feeding pipe and nozzle, described stepper motor II is fixed on framework by flange bracket II, be fixedly connected with small pulley, driven the rotation of large belt wheel by Timing Belt; Described large belt wheel is fixedly connected with rotating shaft, and rotating shaft is with large belt wheel synchronous rotary; Described rotating shaft is provided with head bearing, lower bearing installed surface and annular groove, and it to be fixed between back up pad by metal (upper seat and snap ring and to be provided with register pin above rotating shaft and synchronous rotary thereupon; Described lower bearing is located by lower bearing sleeve; Described head bearing is located by upper bearing sleeve; Described upper bearing sleeve and lower bearing sleeve are individually fixed in back up pad upper and lower end face; Described top chock is provided with optoelectronic switch; It is corresponding with evacuated tube position that described register pin triggers optoelectronic switch position; Described axle sleeve to be fixed in rotating shaft and to revolve maintenance synchronous rotary with rotating shaft, and axle sleeve is provided with sample feeding pipe fixed orifice; Described sample feeding pipe inner chamber is provided with two not connected cavitys, and two cavitys are communicated with peristaltic pump I, peristaltic pump II respectively by emulsion tube, and two cavity outlet ends are respectively provided with a nozzle;
Described collector unit comprises evacuated tube, collection tube, filter screen, O type circle, plug, joint, emulsion tube, solenoid valve, distribution valve and peristaltic pump III, and described evacuated tube is public purge pipe, divides into joint; Described collection tube divides into joint, and is connected with solenoid valve by emulsion tube; Described filter screen is fixed between collection tube inner chamber and plug; Described plug peripheral hardware O type circle, and fixed by interference fit and collection tube lower end bore seal; Emulsion tube is connect under described joint; The described solenoid valve other end connects distribution valve Single port; Described distribution valve output port is connected with peristaltic pump III by emulsion tube.
Described collection tube is tubular housing structure, can arrange multiple by circumference uniform distribution as required; Filter screen experimentally filters needs replaceable different meshes filter screen; Solenoid valve is closed type 2/2-way solenoid valve; Distribution valve is Multicarity conjoined structure, and it shares an output port, in its course of work, by the collection tube sample introduction process of opening or shut electromagnetic valve realization is corresponding with it; Peristaltic pump III is stock layout pump.
The principle of work of apparatus of the present invention is:
First, man-machine interface is arranged the parameter such as sampling depth, acquisition time and is transferred to main control module, main control module sends instructions on stepper motor I, to rotate with it around the stopple coupon on drum and start dive, till the actual grade fed back until the depth transducer at mouth of pipe place is consistent with set depth; Main control module sends instruction to stepper motor II according to set sampling plan, under the connection of Timing Belt, driven the rotation of large belt wheel by small pulley, sample feeding pipe can be realized and turn to corresponding collection tube position by initial position (i.e. evacuated tube) and carry out sample collection; Then, main control module sends instructions to peristaltic pump I and solenoid valve, peristaltic pump III according to institute's setting parameter, water sample is pumped to collection tube by sample feeding pipe, solenoid valve now on this collection tube is in opening and all the other collection tubes is in closed condition, water sample filters through filter screen, water sample after filtration is taken away by peristaltic pump III through distribution valve, and now the required planktonic organism gathered is left on the net; Then, after end to be sampled, shut electromagnetic valve, quantitative fixing agent adds in collection tube by the rotation controlling peristaltic pump II; Finally, sample feeding pipe is rotated back to initial position by control step motor II, by the rotation of peristaltic pump by the emptying rate of liquid process in emulsion tube, and cleans emulsion tube before upper once sampling.
3, beneficial effect
Compared with prior art, beneficial effect of the present invention is as follows:
(1) apparatus of the present invention are programmed by PLC, and can automatically run everything according to parameter preset and realize accurately sampling, automaticity is high, and sampling efficiency is fast, and saves human and material resources cost;
(2) apparatus of the present invention employing depth transducer achieves the accurate control to sampling depth, can carry out stratified sampling to the water body of different depth, makes the environment detecting water sample closer to physical environment;
(3) apparatus of the present invention are by the duration of runs of fixing quantity peristaltic pump, realize the accurate control to drainage amount, overcome the problem that existing most of technology drainage amount error is larger;
(4) apparatus of the present invention are except energy automatically collected specimens, also possess in situ quantitation and add fixing agent function, improve the stability of institute's sample thief.
In sum, apparatus of the present invention are simple and easy to use, and sampling efficiency is high, good reliability.
Accompanying drawing explanation
Fig. 1 is the on-line automatic collection of hydrobiont sample of the present invention and save set one-piece construction schematic diagram.
Fig. 2 is the on-line automatic collection of hydrobiont sample of the present invention and save set front view.
Fig. 3 is the on-line automatic collection of hydrobiont sample of the present invention and save set vertical view.
Fig. 4 is the on-line automatic collection of hydrobiont sample of the present invention and save set evacuated tube and collection tube structure cut-open view.
In figure, 1, control module; 1.1, main control module; 1.2, power supply; 1.3, man-machine interface; 2, support; 2.1, framework; 2.2, back up pad; 2.3, base plate; 2.4, upper plate; 2.5, column; 3, sample injection unit; 3.1, depth transducer; 3.2, stopple coupon; 3.3, drum; 3.4 stepper motors I; 3.5, peristaltic pump I; 3.6 peristaltic pumps II; 3.7 flange brackets I; 3.8 fixing agent; 4.1 stepper motors II; 4.2 flange brackets II; 4.3 small pulley; 4.4 Timing Belt; 4.5 large belt wheels; 4.6 rotating shaft; 4.7 snap ring; 4.8 lower bearing; 4.9 step; 4.10 head bearing; 4.11 top chock; 4.12 register pin; 4.13 optoelectronic switch; 4.14 axle sleeve; 4.15 sample feeding pipe; 4.16 nozzle; 5, collector unit; 5.1 evacuated tube; 5.2 collection tube; 5.3 filter screen; 5.4O type circle; 5.5 plug; 5.6 joint; 5.7 emulsion tube; 5.8 solenoid valve; 5.9 distribution valve; 5.10 peristaltic pump III.
Embodiment
For better the present invention being described, be convenient to understand technical scheme of the present invention, typical but non-limiting embodiment of the present invention is as follows:
As shown in Figure 1, the on-line automatic collection of a kind of hydrobiont sample and save set, this device is made up of control module 1, support 2, sample injection unit 3, allocation units 4 and collector unit 5.
Control module 1 comprises main control module 1.1, power supply 1.2 and man-machine interface 1.3; Described main control module 1.1 is the PLC of self-programming, be connected with stepper motor, depth transducer 3.1, optoelectronic switch 4.13, solenoid valve 5.8 by cable, for automatically controlling sampling depth, the rotation of allocation units sample feeding pipe, the process such as sample introduction and stock layout; Described power supply 1.2 is semibridge system variable-voltage power supply, and input voltage is AC110v ~ 220v, and output voltage is DC5v, 12v and 24v; Man-machine interface 1.3 selects graphic control panel, can automatically sample, the operation such as manual sampling action and optimum configurations.
Support 2 comprises framework 2.1, back up pad 2.2, base plate 2.3, upper plate 2.4 and column 2.5; Described framework 2.1 is cavity body structure main frame, is provided with the mounting hole of control module 1 each several part.Described back up pad is installed in framework; Described base plate is fixed on framework; Described upper plate is connected as a single entity by support and base plate; Described upper plate is provided with circumference uniform distribution collection tube and an evacuated tube; Described base plate is provided with joint poling hole; Described base plate center is provided with rotating shaft passing hole;
Sample injection unit 3 comprises depth transducer 3.1, stopple coupon 3.2, drum 3.3, stepper motor I 3.4, peristaltic pump I 3.5, peristaltic pump II 3.6, flange bracket 3.7, fixing agent 3.8; Wherein depth transducer 3.1 connecting line is around on 3.3 drums, stopple coupon 3.2 tube head part and depth transducer 3.1 synchronization lifting; Described depth transducer 3.1 adopts throw-in type liquid transmitter, powered by power supply 1.2, the entrance pressure hole of the other end can fully contact with water body and the data measured are sent to main control module 1.1 by depth transducer 3.1, the rotation of drum 3.3 is driven to carry out retractable cable according to sampling depth setting by stepper motor I 3.4, till sampling set depth is consistent with the actual depth of water; Described peristaltic pump I 3.5 is all connected with main control module 1.1 with peristaltic pump II 3.6, by the rotation of input pulse or time controling peristaltic pump with stop interval determining the sampling volume of water sample and fixing agent; Connected by flange bracket I 3.7 between described drum 3.3 and stepper motor I 3.4 and be fixed in framework 2.1; Described peristaltic pump I 3.5 is water inlet sample pump; Peristaltic pump II 3.6 is fixing agent pump.
Described allocation units 4 comprise stepper motor II 4.1, flange bracket II 4.2, small pulley 4.3, Timing Belt 4.4, large belt wheel 4.5, rotating shaft 4.6, snap ring 4.7, lower bearing 4.8, step 4.9, head bearing 4.10, top chock 4.11, register pin 4.12, optoelectronic switch 4.13, axle sleeve 4.14, sample feeding pipe 4.15, nozzle 4.16; Described stepper motor II 4.1 is connected and fixed with small pulley 4.3, is driven the rotation of large belt wheel 4.5 by Timing Belt 4.4; Described large belt wheel 4.5 is connected and fixed with rotating shaft 4.6, and rotating shaft 4.6 is with large belt wheel 4.5 synchronous rotary; Described rotating shaft 4.6 is provided with head bearing 4.10, lower bearing 4.8 installed surface and snap ring 4.7 groove; Described head bearing 4.10 is located by upper bearing sleeve; Described lower bearing 4.8 is located by lower bearing sleeve; Described upper bearing sleeve and lower bearing sleeve are individually fixed in back up pad 2.2 upper and lower end face; Described rotating shaft 4.6 is fixed between back up pad 2.2 by metal (upper seat and snap ring 4.7; Described stepper motor II 4.1 is fixed on framework 2.1 by flange bracket II 4.2; Described rotating shaft 4.6 is above provided with register pin 4.12 and synchronous rotary thereupon; Described top chock is provided with optoelectronic switch 4.13; It is corresponding with evacuated tube 5.1 position that described register pin 4.12 triggers optoelectronic switch 4.13 position; Described axle sleeve 4.14 to be fixed in rotating shaft 4.6 and to keep synchronous rotary with rotating shaft 4.6; Described axle sleeve 4.14 is provided with sample feeding pipe 4.15 fixed orifice; Described sample feeding pipe 4.15 is fixed on axle sleeve 4.14; Described sample feeding pipe 4.15 inner chamber is provided with two not connected cavitys, and two cavity outlet ends are respectively provided with a nozzle 4.16; Described sample feeding pipe 4.15 liang of cavitys are communicated with peristaltic pump I 3.5, peristaltic pump II 3.6 respectively by emulsion tube 5.7; Described peristaltic pump I 3.5 is connected with water sample, and peristaltic pump II 3.6 is connected with fixing agent.
Collector unit 5 comprises evacuated tube 5.1, collection tube 5.2, filter screen 5.3, O type circle 5.4, plug 5.5, joint 5.6, emulsion tube 5.7, solenoid valve 5.8, distribution valve 5.9, peristaltic pump III 5.10; Described evacuated tube 5.1 is public purge pipe, divides into joint 5.6, and joint connects emulsion tube 5.7 5.6 times; Described collection tube 5.2 can arrange multiple by circumference uniform distribution as required, and collection tube 5.2 is tubular housing structure; Described plug 5.5 peripheral hardware O type circle 5.4, and fixed by interference fit and collection tube 5.2 lower end bore seal; Described filter screen 5.3 is fixed between collection tube 5.2 inner chamber and plug 5.5, and filter screen 5.3 experimentally filters the filter screen needing replaceable different meshes; Described collection tube 5.2 is divided into joint 5.6 and is connected with solenoid valve 5.8 by emulsion tube 5.7; Described solenoid valve 5.8 other end connects distribution valve 5.9 Single port; Described solenoid valve 5.8 is closed type 2/2-way solenoid valve; Described distribution valve 5.9 is Multicarity conjoined structure, and it shares an output port; Output port is connected with peristaltic pump III 5.10 by emulsion tube 5.7; In its course of work, realize collection tube 5.2 sample introduction process corresponding with it by unlatching or shut electromagnetic valve 5.8; Described peristaltic pump III 5.10 is stock layout pump.
The principle of work of apparatus of the present invention: single sample gatherer process mainly comprises depth adjustment, allocation units sample feeding pipe rotates to specifying collection tube, continuous sample introduction and stock layout, interpolation fixing agent, allocation units sample feeding pipe to return back to emptying five actions of evacuated tube, the new sample of each collection all needs the above action of repetition.The power of all electric devices provides by power supply 1.2.
First, man-machine interface 1.3 is arranged the parameter such as sampling depth, acquisition time and is transferred to main control module 1.1, main control module 1.1 sends instructions on stepper motor I 3.4, to rotate with it around the stopple coupon 3.2 on drum 3.3 and start dive, till the actual grade fed back until the depth transducer 3.1 at mouth of pipe place is consistent with set depth;
Secondly, main control module 1.1 sends instruction to stepper motor II 4.1 according to set sampling plan, under the connection of Timing Belt 4.4, driven the rotation of large belt wheel 4.5 by small pulley 4.3, sample feeding pipe 4.15 can be realized and turn to corresponding collection tube 5.2 position by initial position (i.e. evacuated tube 5.1) and carry out sample collection; Then, main control module 1.1 sends instructions to peristaltic pump I 3.5 and solenoid valve 5.8, peristaltic pump III 5.10 according to institute's setting parameter, water sample passes through, sample feeding pipe 4.15 is pumped to collection tube 5.2, solenoid valve 5.8 now on this collection tube 5.2 is in opening and all the other collection tubes 5.2 is in closed condition, water sample filters through filter screen 5.3, and the water sample after filtration is taken away by peristaltic pump III 5.10 through distribution valve 5.9, and now the required planktonic organism gathered is left on the net;
Then, after end to be sampled, shut electromagnetic valve 5.8, quantitative fixing agent adds in collection tube 5.2 by the rotation controlling peristaltic pump II 3.6;
Finally, sample feeding pipe 4.15 is rotated back to initial position by control step motor II 4.1, by the rotation of peristaltic pump by the emptying rate of liquid process in emulsion tube 5.7, and cleans emulsion tube before upper once sampling.
Certainly, above-mentioned explanation is not limitation of the present invention, and in essential scope of the present invention, the change made, remodeling, interpolation or replacement, all should belong to protection scope of the present invention.

Claims (8)

1. the on-line automatic collection of hydrobiont sample and save set, it is characterized in that, described device comprises control module (1), support (2), sample injection unit (3), allocation units (4) and collector unit (5); Wherein:
Described control module (1) comprises main control module (1.1), power supply (1.2) and man-machine interface (1.3);
Described support (2) comprises framework (2.1), back up pad (2.2), base plate (2.3), upper plate (2.4) and column (2.5), and described back up pad (2.2) is installed in framework (2.1); Described base plate (2.3) is fixed on framework (2.1); Described upper plate (2.4) is connected as a single entity by column (2.5) and base plate (2.3);
Described sample injection unit (3) comprises depth transducer (3.1), stopple coupon (3.2), drum (3.3), stepper motor I (3.4), peristaltic pump I (3.5), peristaltic pump II (3.6), flange bracket I (3.7) and fixing agent (3.8), described depth transducer (3.1) connecting line around on drum (3.3), stopple coupon (3.2) tube head part and depth transducer (3.1) synchronization lifting; Described drum (3.3) and stepper motor I (3.4) are connected by flange bracket I (3.7) and are fixed in framework (2.1); Described peristaltic pump I (3.5) is connected with water sample sample feeding pipe; Described peristaltic pump II (3.6) is connected with fixing agent (3.8) sample feeding pipe;
Described allocation units (4) comprise stepper motor II (4.1), flange bracket II (4.2), small pulley (4.3), Timing Belt (4.4), large belt wheel (4.5), rotating shaft (4.6), snap ring (4.7), lower bearing (4.8), step (4.9), head bearing (4.10), top chock (4.11), register pin (4.12), optoelectronic switch (4.13), axle sleeve (4.14), sample feeding pipe (4.15) and nozzle (4.16), described stepper motor II (4.1) is fixedly connected with small pulley (4.3), the rotation of large belt wheel (4.5) is driven by Timing Belt (4.4), described large belt wheel (4.5) is fixedly connected with rotating shaft (4.6), and rotating shaft (4.6) is with large belt wheel (4.5) synchronous rotary, described rotating shaft (4.6) to be fixed between back up pad (2.2) by metal (upper seat and snap ring (4.7) and rotating shaft (4.6) is provided with register pin (4.12) above, described top chock (4.11) is provided with optoelectronic switch (4.13), described axle sleeve (4.14) is fixed on rotating shaft (4.6) and goes up and keep synchronous rotary with rotating shaft (4.6), and axle sleeve (4.14) is provided with sample feeding pipe (4.15) fixed orifice, described sample feeding pipe (4.15) inner chamber is provided with two not connected cavitys, and two cavitys are communicated with peristaltic pump I (3.5), peristaltic pump II (3.6) respectively, and two cavity outlet ends are respectively provided with a nozzle (4.16),
Described collector unit (5) comprises evacuated tube (5.1), collection tube (5.2), filter screen (5.3), O type circle (5.4), plug (5.5), joint (5.6), emulsion tube (5.7), solenoid valve (5.8), distribution valve (5.9) and peristaltic pump III (5.10), described evacuated tube (5.1) divides into joint (5.6), and position and register pin (4.12) to trigger optoelectronic switch (4.13) position corresponding; Described collection tube (5.2) divides into joint (5.6), is connected with solenoid valve (5.8) by emulsion tube (5.7); Described filter screen (5.3) is fixed between collection tube (5.2) inner chamber and plug (5.5); Described plug (5.5) peripheral hardware O type circle (5.4), and fixed by interference fit and collection tube (5.2) lower end bore seal; Emulsion tube (5.7) is connect under described joint (5.6); Described solenoid valve (5.8) other end connects distribution valve (5.9) Single port; Described distribution valve (5.9) output port is connected with peristaltic pump III (5.10) by emulsion tube (5.7).
2. the on-line automatic collection of hydrobiont sample according to claim 1 and save set, it is characterized in that, the PLC that the main control module (1.1) of described device control units (1) is self-programming, is connected with man-machine interface (1.3), depth transducer (3.1), stepper motor (3.4), optoelectronic switch (4.13), solenoid valve (5.8) by cable.
3. the on-line automatic collection of hydrobiont sample according to claim 1 and save set, it is characterized in that, the power supply (1.2) of described device control units (1) is semibridge system variable-voltage power supply, input voltage is AC110v ~ 220v, and output voltage is DC5v, 12v and 24v.
4. the on-line automatic collection of hydrobiont sample according to claim 1 and save set, it is characterized in that, the man-machine interface (1.3) of described device control units (1) selects graphic control panel, can automatically sample, manual sampling action and parameter setting operation.
5. the on-line automatic collection of hydrobiont sample according to claim 1 and save set, is characterized in that, described appliance stand (2) is little by density, and the resistant material that intensity is high is made.
6. the on-line automatic collection of hydrobiont sample according to claim 1 and save set, it is characterized in that, the framework (2.1) of described appliance stand (2) is cavity body structure main frame, is provided with control module (1) mounting hole and man-machine interface (1.3) mounting hole; Described base plate (2.3) is provided with joint poling hole, and base plate (2.3) center is provided with rotating shaft passing hole; Described upper plate (2.4) is provided with circumference uniform distribution collection tube and round determines hole.
7. the on-line automatic collection of hydrobiont sample according to claim 1 and save set, it is characterized in that, the depth transducer (3.1) of described device sample injection unit (3) adopts throw-in type liquid transmitter, powered by power supply (1.2), the entrance pressure hole of the other end can fully contact with water body and the data measured are sent to main control module (1.1) by sensor, the rotation of drum (3.3) is driven to carry out retractable cable according to sampling depth setting by stepper motor (3.4), till sampling set depth is consistent with the actual depth of water; Described peristaltic pump I (3.5) is all connected with main control module (1.1) with peristaltic pump II (3.6), by the rotation of input pulse or time controling peristaltic pump with stop interval determining the sampling volume of water sample and fixing agent; Described peristaltic pump I (3.5) is water inlet sample pump; Described peristaltic pump II (3.6) is for entering fixing agent pump.
8. the on-line automatic collection of hydrobiont sample according to claim 1 and save set, it is characterized in that, the collection tube (5.2) of described device collector unit (5) is tubular housing structure, can arrange multiple by circumference uniform distribution as required; Filter screen (5.3) experimentally filters needs can more different meshes filter screen; Solenoid valve (5.8) is closed type 2/2-way solenoid valve; Distribution valve (5.9) is Multicarity conjoined structure, and it shares an output port, in its course of work, by the collection tube sample introduction process of opening or shut electromagnetic valve realization is corresponding with it; Peristaltic pump III (5.10) is stock layout pump.
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