CN1054190A - From metabolic product of staphylococcus aureus, extract the method for treatment malignant tumor medication - Google Patents
From metabolic product of staphylococcus aureus, extract the method for treatment malignant tumor medication Download PDFInfo
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- CN1054190A CN1054190A CN91106067A CN91106067A CN1054190A CN 1054190 A CN1054190 A CN 1054190A CN 91106067 A CN91106067 A CN 91106067A CN 91106067 A CN91106067 A CN 91106067A CN 1054190 A CN1054190 A CN 1054190A
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Abstract
The invention discloses a kind of metabolite and extract the method for treatment malignant tumor medication with staphylococcus aureus, the product of this method is called for short " BM828 " has direct and indirect lethal effect to malignant cell, and the culture medium main component adopts peptone, pig heart infusion, sheep liver soaking liquid preparation.Cultivate strain with the meat soup recovery; make the protective agent of bacterial strain with skim milk; autoclaving does not add any antiseptic, through heat treatment, merceration go out, modes such as charcoal treatment, aseptic filtration make injection, have the significant effect of enhancing human body immunity function significantly.
Description
The present invention relates to biological response modifier and promptly extract the method for prevention and the medication of treatment malignant tumor with metabolic product of staphylococcus aureus.The product of this method has direct killing effect to malignant cell, can also increase substantially body's immunological function, suppresses indirectly and kills tumor cell, significantly repairs normal tissue cell, leukocyte increasing, hematoblastic quantity and function.Described biological response modifier is meant the material that can significantly strengthen, promote or recover human body defence capability (immunity), these material general designation biological response modifier, and the product of described this method abbreviates " BM828 " as.
Entrust auditor's (entrusting numbering 91019) of four ones of State Patent Office's examinations to propose following corresponding document by online information retrieval.JP55-8730 discloses a kind of medicament that is used for bronchial allergy, method is respectively to cultivate each Bacillus to gather up cyton, and comes cell killing with heating, reuse " formalin " sterilization, then, be suspended in the normal saline solution, so just make the dead cell base soln, injection is made in the base soln mixing of gained, it just can be used as a kind of anti-virus formulation, its weak point, the product quality that this method makes is not good enough, poor stability, and certain side effect is arranged.
Another piece is that JP5445161 discloses a kind of immunopotentiator that is used to prevent and treat malignant tumor, and its method is that the component of gained is a kind of immunopotentiator with after golden cell of staphylococcus or bacterial body pulverizing, filtering out solid constituent.Can be used for preventing and treating effectively the symptom that the immunity function of cell is lowered, mainly treat viral infection.
Outside the deficiency: antibacterial is pulverized earlier and refilters, and filtrate composition complexity filters out solid, contains the thin material of pulverizing in the liquid, is not difficult to expect that side effect is big, though immunological enhancement is arranged, to the oncotherapy effect with brighten cytosis and do not have effect.
Purpose of the present invention discloses in order to overcome the deficiencies in the prior art part that a kind of technology is simple, reliable in quality, the minimum metabolite with staphylococcus aureus of toxic and side effects are extracted the method for treatment malignant tumor medication.
Purpose of the present invention realizes by following scheme:
Method of the present invention is described by technological process can divide three parts:
The preparation of first's strain; The second portion medium preparation; Third part merges the preparation of inoculated and cultured, fermentation, extraction, preparation.
The preparation of first's strain:
1, recovery is cultivated: staphylococcus aureus is inoculated in the Nutrient agar inclined-plane of lixivium of pig heart preparation cultivated 18 hours for 35 ℃;
2, packing: it is an amount of to get slant culture, evenly grinds in the 1ml skim milk and makes the suspension branch aseptic strain pipe of packing into, every pipe 0.3ml;
3, freeze: the strain pipe that bacteria suspension will be housed was put in-30 ℃ of low temperature ethanol quick-freezing one hour;
4, low pressure is drained: the strain pipe after will freezing is put and is contained dry CaCl
2Connect vacuum pump in the vacuum desiccator, vacuum is drained (needing seven hours approximately);
5, sealing was connected on the above-mentioned strain pipe of draining on the many menifolds of rubber the about 10-20 of vacuum suction minute flame sealed tube mouth under vacuum condition.
The preparation of second portion culture medium:
Main component peptone 0.5-2g%, sodium chloride 0.3-0.9g%, thiamine 0.0-0.05g%, pig heart infusion 5-20ml%, sheep liver soaking liquid 5-30ml%.
1, pig heart infusion preparation: get fresh Sanguis sus domestica, remove clot, fat, fascia cleans, cuts with scissors injection broken, that weigh, add qdx with tap water, fresh double distilled water and put refrigerator (4 ℃) 14 hours with fresh double distilled water, boil and used eight layers of husky cloth coarse filtration in two hours while hot, reuse buchner funnel quantitative filter paper sucking filtration, it is standby to clarify yellow liquid.
2, sheep liver soaking liquid is used with quadrat method and is prepared;
3, the amount of making up a prescription as required takes by weighing peptone, sodium chloride, with fresh double distilled water heated solution;
4, the amount of making up a prescription is measured (absorption) lixivium of pig heart, Hepar Caprae seu ovis leachate and thiamine as required;
5,3.4 solution are mixed, add injection with fresh double distilled water to aequum;
6, transfer PH to 3-4 to add the 0.5g% injection, boil (not stopping to stir) half an hour with Powdered high-quality activated carbon;
7, with the decarburization of buchner funnel quantitative filter paper sucking filtration;
8, transferring PH to 7-9 to add the 0.5g% injection boils half an hour once more with Powdered high-quality activated carbon;
9, sucking filtration decarburization;
10, transfer PH to 6.0-7.5 packing 500ml(cleaning solution handle, double distilled water clean 180 ℃ do removed thermal source in roasting two hours) saline bottle adds aluminium lid and rolls mouth;
11,15 pounds of sterilizations in 30 minutes of high pressure are standby.
Third part
Concentration 10000-20 hundred million/ml is adopted in inoculation, and ratio 0.5-5ml% cultivates at 30 ℃-38 ℃, 18-72/ hour, adjusts PH6-7.6, aseptic filtration microporous filter membrane 0.22 μ.
But the high poly-golden Fructus Vitis viniferae (HAS) of this bacterial strain called after is a kind of new bacterial strain of high-efficiency low-toxicity, had been sent to China Committee for Culture Collection of Microorganisms common micro-organisms center before the applying date microbiological specimens is provided.
1, providing the claimant of microbiological specimens is that the legal representative of Xiehe Biological Agent Technology Co., Ltd., Shenyang is old huge surplus, No. 8, three good streets, peace zone, Shenyang City, address, 9 first, and in March, 1991 time, preserving number is waited to look into, and brief description is as follows:
High poly-golden Portugal bacterium is to separate in the 105 strain staphylococcuses that obtain to screen from the various Pathologic specimens that each section of hospital clinical send Bacteriology Room to check; store method-the vacuum freeze-drying method of bacterial strain; protective agent, skim milk; should long preservation keep the constant purpose of bacterial strain hereditism character again in order to reach; except selecting effectively stable method and cryodesiccated effect being detected at every turn, also to identify the bacterial strain of recovery comprehensively.
Toxicological test:
For reagent liquid is 20 times of concentrated wiring liquids of clinical usefulness " BM828 " injection, white mice body weight 17-20 gram/only, female, male half and half is healthy no pregnant, get 20 and be divided into two groups at random by the sex body weight, every group 10, the normal control group is not given any medicine, after the above-mentioned test liquid 0.5ml of every mice by intraperitoneal injection of the test group administration, white mice does not have any upright reaction of sending out, observed seven days, white mice is strong deposits, and takes by weighing body weight, and disconnected neck is put to death, dissect perusal, each main organs is not seen obvious pathological change, wins the heart, liver, spleen, two kidneys are weighed respectively, calculate the heart-body ratio of each animal, liver-body ratio, spleen-body ratio, kidney-body ratio, related data to normal matched group and administration group carries out test of significance, above-mentioned internal organs are fixedly made tissue slice routinely, under optical microscope, observe, each main organs (heart of matched group and administration group, liver, spleen, kidney) there is no tangible Histological change.
Conclusion: administration is analyzed the no abnormal finding of each main internal organs of mice and is compared with the normal control group after seven days, and the white mice speed of growth, the heart-body ratio, liver-body ratio, spleen-body ratio, kidney one are than also no abnormality seen change of the histological examination of all not having significant difference (P770.05) conscience spleen kidney.
Every mice of this experiment is all injected 0.5ml, restrains body weight calculating if press 20, and quite 20 times of concentrated wiring liquid 25ml/kg of mice by intraperitoneal injection are converted into injection 500ml/kg, and this dosage is 50000 times of people's clinical dosage by kg body weight calculating.
Clinical trial:
Units such as Chinese Medical Sciences University institute of oncology, 307 hospitals of institute of section of army, tumor portion of Anshan City Central Hospital, the People's Armed Police of Liaoning Province hospital oncology have done clinical practice respectively.
For example: Anshan City Central Hospital's tumor in-patient department is used " BM828 " patients such as primary hepatocarcinoma, breast carcinoma, colon cancer, cerebroma, cancerometastasis lymph node is carried out immunization therapy, tracking monitor acquisition satisfactory result, as after treating 1 month, NK cell (improving index of immunologic function) increases the 162-312% solid tumor and dwindles 50%-75%.
1) case is selected:
Li Ming man 31 years old
Suffer from meningioma, perform the operation in total institute of Shenyang ground force in October, 1989, take out two 2 * 3 centimeters tumor, August nineteen ninety the disease relapse second operation, take out 3 * 4 centimeters tumors again, postoperative was less than 4 months, recurrence paralysis is for the third time haveed no alternative but perform the operation for the third time again, and cancerous cell extensively shifted and only took down a part of carcinoma this moment, when patient accepts BM828 after course of treatment, the state of an illness just is under control, and symptom obviously alleviates spirit and takes a turn for the better day by day, the same with the normal person now the feed, can leave the bed and walk, even do some light work.
Anshan 20 routine malignant tumor patients carry out immunization therapy and all obtain promising result.
2) therapeutic scheme:
BM828 is little yellow, and clarifying sterilization biological preparation does not contain antiseptic, and is without any side effects, and injection 2ml/ props up, and contains the intramuscular injection of 1000 units, injects once in 1-2 days, and 30 times is a course of treatment.
3) criterion of therapeutical effect:
Produce effects: malignant tumor there is direct killing effect, can also be significantly, improve body's immunological function and suppress indirectly and kill tumor cell, leukocyte increasing, hematoblastic effect.
The data that Anshan central hospital provides, effective percentage 90% has no side effect.
Tumour hospital of PLA Academy of Military Sciences witnesses, through the animal test of pesticide effectiveness, and 1500 units of local application/once as a result, inhibitory rate 91%.
4) therapeutic outcome:
Xu Hongying: left breast cancer, left axillary lymph is carried down and is moved, and course of treatment of 4 * 3 kilograms of medications of lump, NK cell showed increased reaches 413%, and after lymph node dwindled 2/3, two course of treatment, lump disappeared substantially.
Wang Guisheng: primary hepatocarcinoma, portal vein tumor thrombus, high pressure, a large amount of ascites, edema of lower limbs, all recover normal ascites and obviously reduce edema of pair of lower extremities and disappear at two courses of treatment of medication.
Luo Changman: esophageal carcinoma, thoracic vertebra shift, after medication two courses of treatment, and NK cell showed increased, the coarse foci disappearance of the esophageal mucosa membrane injury of admiring.
Li Enquan: the nasopharyngeal carcinoma recurrence, bone shifts, after treating two courses of treatment, the NK cytosis, left rib lump disappears, pain relief, cervical region is movable to be increased.
Suffice to show that more than BM828 has significant curative effect, compare to have suitable superiority with chemotherapy.
The method for preparing biological regulator with staphylococcus aureus having thus described the invention is a kind of method economically and reasonably, because the composition of this method is all got fine kind, do not add any antiseptic, it is again sterilization preparation, without any side effects, its reliable degree particularly adopts the BM828 parenteral solution of preparation of this law preparation considerably beyond general biological preparation, quality and stable curative effect, it is good that lucifuge cool dark place preservation did not precipitate clarity in 2 years.Without any side effects fully through the toxicity Inspection Certificate, pharmacological verification not only confirms the effect of remarkable enhancing human body immunity function, as normal mouse injection BM828(650 unit/Mus), the activity of natural killer cell (NK cell) increases by 232% before than administration, in suffering from lung cancer in mice, inject, the NK activity is than matched group behind this medicine, increase by 304%, lymhocyte transformation rate increase by 158% etc., also very desirable to killing and wounding of malignant cell, the zoopery result confirms pulmonary carcinoma, cervical cancer, the malignant tumor of digestive tract tumour inhibiting rate has met or exceeded international standard between 30%-40%.
Embodiment: 1 describe in conjunction with the accompanying drawings, accompanying drawing 1 is a process chart of the present invention.Bright for instance step:
The preparation of embodiment 1 first's strain:
1, recovery is cultivated: staphylococcus aureus is inoculated in the Nutrient agar inclined-plane of lixivium of pig heart preparation cultivated 18 hours for 35 ℃;
2, packing: it is an amount of to get slant culture, evenly grinds in the 1ml skim milk and makes the suspension branch aseptic strain pipe of packing into, every 0.3ml;
3, freeze: the strain pipe that bacteria suspension will be housed was put in-30 ℃ of low temperature ethanol quick-freezing one hour;
4, low pressure is drained: the strain pipe after will freezing is put and is contained dry CaCl
2Connect vacuum pump in the vacuum desiccator, vacuum is drained (needing seven hours approximately);
5, sealing was connected on the above-mentioned strain pipe of draining on the many menifolds of rubber the about 10-20 of vacuum suction minute flame sealed tube mouth under vacuum condition.
The preparation of second portion culture medium:
Main component peptone 0.5g%, sodium chloride 0.3g%, thiamine 0.0g%, pig heart infusion 5ml%, sheep liver soaking liquid 5ml%.
1, pig heart infusion preparation: get fresh Sanguis sus domestica, remove clot, fat, fascia cleans, cuts with scissors injection broken, that weigh, add qdx with tap water, fresh double distilled water and put refrigerator (4 ℃) 14 hours with fresh double distilled water, boil two hours while hot with going into the husky cloth coarse filtration of layer, reuse buchner funnel quantitative filter paper sucking filtration, it is standby to clarify yellow liquid.
2, sheep liver soaking liquid is used with quadrat method and is done;
3, the amount of making up a prescription as required takes by weighing peptone, sodium chloride, with fresh double distilled water heated solution;
4, the amount of making up a prescription is measured (absorption) lixivium of pig heart, Hepar Caprae seu ovis leachate and thiamine as required;
5,3.4 solution are mixed, add injection with fresh double distilled water to aequum;
6, transfer PH to 3-4 to add the 0.5g% injection, boil (not stopping to stir) half an hour with Powdered high-quality activated carbon;
7, with the decarburization of buchner funnel quantitative filter paper sucking filtration;
8, transferring PH to 7-9 to add the 0.5g% injection boils half an hour once more with Powdered high-quality activated carbon;
9, sucking filtration decarburization;
10, transfer PH to 6.0-7.5 packing 500ml(cleaning solution handle, double distilled water clean 180 ℃ do removed thermal source in roasting two hours) saline bottle adds aluminium lid and rolls mouth;
11,15 pounds of sterilizations in 30 minutes of high pressure are standby.
Third part
Concentration 10000-20 hundred million/ml is adopted in inoculation, and ratio 0.5ml% cultivates at 30 ℃-38 ℃, during 18-72/, adjusts PH6-7.6, degerming 1, degerming 2 microporous filter membrane 0.22 μ.
The preparation of embodiment 2 first's strains:
1, recovery is cultivated: staphylococcus aureus is inoculated in the Nutrient agar inclined-plane of lixivium of pig heart preparation cultivated 18 hours for 35 ℃;
2, packing: it is an amount of to get slant culture, evenly grinds in the 1ml skim milk and makes the suspension branch aseptic strain pipe of packing into, every 0.3ml;
3, freeze: the strain pipe that bacteria suspension will be housed was put in-30 ℃ of low temperature ethanol quick-freezing one hour;
4, low pressure is drained: the strain pipe after will freezing is put and is contained dry CaCl
2Connect vacuum pump in the vacuum desiccator, vacuum is drained (needing seven hours approximately);
5, sealing was connected on the above-mentioned strain pipe of draining on the many menifolds of rubber the about 10-20 of vacuum suction minute flame sealed tube mouth under vacuum condition.
The preparation of second portion culture medium:
Main component peptone 2g%, sodium chloride 0.9g%, thiamine 0.05g%, pig heart infusion 20ml%, sheep liver soaking liquid 30ml%.
1, pig heart infusion preparation: get fresh Sanguis sus domestica, remove clot, fat, fascia cleans, cuts with scissors injection broken, that weigh, add qdx with tap water, fresh double distilled water and put refrigerator (4 ℃) 14 hours with fresh double distilled water, boil two hours while hot with going into the husky cloth coarse filtration of layer, reuse buchner funnel quantitative filter paper sucking filtration, it is standby to clarify yellow liquid.
2, sheep liver soaking liquid is used with quadrat method and is done;
3, the amount of making up a prescription as required takes by weighing peptone, sodium chloride, with fresh double distilled water heated solution;
4, the amount of making up a prescription is measured (absorption) lixivium of pig heart, Hepar Caprae seu ovis leachate and thiamine as required;
5,3.4 solution are mixed, add injection with fresh double distilled water to aequum;
6, transfer PH to 3-4 to add the 0.5g% injection, boil (not stopping to stir) half an hour with Powdered high-quality activated carbon;
7, with the decarburization of buchner funnel quantitative filter paper sucking filtration;
8, transferring PH to 7-9 to add the 0.5g% injection boils half an hour once more with Powdered high-quality activated carbon;
9, sucking filtration decarburization;
10, transfer PH to 6.0-7.5 packing 500ml(cleaning solution handle, double distilled water clean 180 ℃ do removed thermal source in roasting two hours) saline bottle adds aluminium lid and rolls mouth;
11,15 pounds of sterilizations in 30 minutes of high pressure are standby.
Third part
Concentration 10000-20 hundred million/ml is adopted in inoculation, and ratio 5ml% cultivates at 30 ℃-38 ℃, 18-72 hour, adjusts PH6-7.6, degerming 1, degerming 2 microporous filter membrane 0.22 μ.
Claims (2)
1, a kind of biological response modifier that is used for malignant tumor prevention and treatment, described biological response modifier is to extract from the metabolite of staphylococcus aureus, its feature can be summarized as following steps: bacterial strain recovery is cultivated, culture medium refining, above-mentioned two kinds of liquid are merged, at concentration 10000-20 hundred million/ml, ratio 0.5-5ml% is inoculation down, at 30 ℃-38 ℃, cultivate under the 18-72/ hour condition, transfer pH value 6-7.6, use 0.22 μ, microporous filter membrane removes the secondary bacterium, again by circulation steam autoclaving.Described recovery cultural method is:
Lyophilization pipe after inject opening with meat soup makes into suspension, is inoculated on the blood plate 35 ℃ then and cultivates and observed its survival condition in 24 hours.
(1) cultivates: staphylococcus aureus Z
2Inoculation was cultivated 18 hours for 35 ℃ in the nutrition margarine inclined-plane with the lixivium of pig heart preparation;
(2) packing: it is an amount of to get slant culture, evenly grinds in the 1ml skim milk and makes the suspension branch aseptic strain pipe of packing into, every pipe 0.3ml;
(3) freeze: the strain pipe that bacteria suspension will be housed was put in-30 ℃ of low temperature ethanol quick-freezing one hour;
(4) low pressure is drained: the strain pipe after will freezing is put and is contained dry CaCl
2Vacuum desiccator in, connect vacuum pump, vacuum is drained (approximately need seven hours);
(5) sealing: the above-mentioned strain pipe of draining is connected on the many menifolds of rubber, the about 10-20 of vacuum suction minute, flame sealed tube mouth under vacuum condition;
Described culture medium process for purification is:
Refining culture medium:
Peptone 0.5-2%
Sodium chloride 0.3-0.9%
Thiamine 0.01-0.05%
Pig heart infusion 5-20ml%
Sheep liver soaking liquid 5-30ml%
Making with extra care of PGH culture medium:
(1) pig heart infusion preparation: get fresh Sanguis sus domestica, remove clot, fat and fascia, clean with tap water, fresh double distilled water, cut with scissors broken, weigh, the injection that adds doubling dose was put refrigerator (4 ℃) 14 hours with fresh double distilled water, boiled and used eight layers of gauze coarse filtration in two hours while hot, reuse buchner funnel quantitative filter paper sucking filtration, it is standby to clarify yellow filtrate;
(2) sheep liver soaking liquid preparation: remove clot, fat and fascia, clean with tap water, fresh double distilled water, cut with scissors broken, weigh, the injection that adds doubling dose was put refrigerator (4 ℃) 14 hours with fresh double distilled water, boil and used eight layers of gauze coarse filtration in two hours while hot, reuse buchner funnel quantitative filter paper sucking filtration, it is standby to clarify yellow filtrate;
(3) amount of making up a prescription as required takes by weighing peptone, sodium chloride, with fresh double distilled water heating for dissolving;
(4) amount of making up a prescription as required, (absorption) lixivium of pig heart, new liver leachate and thiamine;
(5) 3,4 solution are mixed, add injection with fresh double distilled water to aequum;
(6) transfer PH to 3.5 to add the 3.5g% injection and boil (not stopping to stir) half an hour with Powdered high-quality activated carbon;
(7) with buchner funnel quantitative filter paper sucking filtration, decarburization;
(8) transfer PH to 8.5 to add the 0.5g% injection and boil (not stopping to stir) half an hour once more with Powdered high-quality activated carbon;
(9) successively with buchner funnel quantitative filter paper, G
4The decarburization of glass filter sucking filtration;
(10) transfer PH to 6.2 packing 500ml (cleaning solution is handled, double distilled water clean 180 ℃ do removed thermal source in roasting two hours) saline bottle adds aluminium lid and rolls mouth;
(11) 15 pounds of sterilizations in 30 minutes of high pressure are standby.
2, according to the method that proposes the medication of treatment malignant tumor in the described metabolic product of staphylococcus aureus of claim 1, it is characterized in that:
Bacterial strain preservation-vacuum freeze-drying method, protective agent skim milk peptone are substrate with milk, and Cor Sus domestica, Hepar Caprae seu ovis are limit the healthy raw material in stripped four hours, sodium hydroxide, thiamine hydrochloride, activated carbon, sodium chloride, distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN91106067A CN1023567C (en) | 1991-04-01 | 1991-04-01 | Method for extracting medicine for treating malignant tumor from staphylococcus aureus metabolite |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN91106067A CN1023567C (en) | 1991-04-01 | 1991-04-01 | Method for extracting medicine for treating malignant tumor from staphylococcus aureus metabolite |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1054190A true CN1054190A (en) | 1991-09-04 |
| CN1023567C CN1023567C (en) | 1994-01-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN91106067A Expired - Lifetime CN1023567C (en) | 1991-04-01 | 1991-04-01 | Method for extracting medicine for treating malignant tumor from staphylococcus aureus metabolite |
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| Country | Link |
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| CN (1) | CN1023567C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061537C (en) * | 1996-09-26 | 2001-02-07 | 张茂良 | Monascus preparation for hyperlipemia and relevant cardiac and cerebral diseases |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1108170C (en) * | 1996-10-11 | 2003-05-14 | 李绍光 | Buffer solution of staphylococcus aureus liquid and staphylococcus aureus liquid with buffer solution |
-
1991
- 1991-04-01 CN CN91106067A patent/CN1023567C/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061537C (en) * | 1996-09-26 | 2001-02-07 | 张茂良 | Monascus preparation for hyperlipemia and relevant cardiac and cerebral diseases |
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| Publication number | Publication date |
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| CN1023567C (en) | 1994-01-19 |
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Assignee: Xiehe Biological Pharmaceutical Co., Ltd., Shenyang Assignor: Xiehe Group Co., Ltd., Shenyang Contract fulfillment period: 2009.11.6 to 2011.3.31 Contract record no.: 2009210000271 Denomination of invention: Process for extracting malignant tumor medicine from metabolite of staphylococcus aureus Granted publication date: 19931225 License type: General permission Record date: 20091110 |
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Free format text: COMMON LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2009.11.6 TO 2011.3.31; CHANGE OF CONTRACT Name of requester: SHENYANG XIEHE BIOLOGICAL PHARMACEUTICAL CO., LTD. Effective date: 20091110 |
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