CN105418772A - Method for grading dextrin - Google Patents
Method for grading dextrin Download PDFInfo
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- CN105418772A CN105418772A CN201510697479.3A CN201510697479A CN105418772A CN 105418772 A CN105418772 A CN 105418772A CN 201510697479 A CN201510697479 A CN 201510697479A CN 105418772 A CN105418772 A CN 105418772A
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- dextrin
- classification
- filter cake
- supernatant liquor
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
- C08B30/12—Degraded, destructured or non-chemically modified starch, e.g. mechanically, enzymatically or by irradiation; Bleaching of starch
- C08B30/18—Dextrin, e.g. yellow canari, white dextrin, amylodextrin or maltodextrin; Methods of depolymerisation, e.g. by irradiation or mechanically
Abstract
The invention provides a method for grading dextrin, and belongs to the field of food processing. The method comprises the steps of: placing dextrin in an alcohol solution with concentration of 0%, stirring for 4-6 hours at the temperature of 20-25 DEG C, performing centrifugalization, and drying the supernate to obtain graded dextrin 1; placing a filter cake in an alcohol solution with the concentrate of 60%, stirring for 4-6 hours at the temperature of 20-25 DEG C, performing centrifugalization, and drying the supernate to obtain graded dextrin 2; placing the filter cake in an alcohol solution with the concentrate of 40%, stirring for 4-6 hours at the temperature of 20-25 DEG C, performing centrifugalization, and drying the supernate to obtain graded dextrin 3; placing the filter cake in an alcohol solution with the concentrate of 20%, stirring for 4-6 hours at the temperature of 20-25 DEG C, performing centrifugalization, and drying the supernate to obtain graded dextrin 4; placing the filter cake in distilled water, stirring for 4-6 hours at the temperature of 20-25 DEG C, performing centrifugalization, and drying the supernate to obtain graded dextrin 5; and washing and drying the filter cake to obtain graded dextrin 6 so as to obtain 5-6 graded dextrin components in this way. The method is simple, large in handling capacity and low in cost.
Description
Technical field
A method for classification dextrin, relates to the alcohol progressively application of dissolving technology on classification dextrin, belongs to food processing technology field.
Background technology
At present, polysaccharide stage division mainly comprises chromatographic fractionation method, membrane filtration staging, temperature classification method and alcohol precipitation staging.The fractional efficiency of chromatographic fractionation method depends primarily on the character of filler, and this method cost is high, treatment capacity is little, be difficult to realize industrialization.The grading effect of membrane filtration depends on the performance of film, and this method poor repeatability, cost is high.And in temperature classification process, palpus strict temperature control, requires high to plant and instrument.Comparatively speaking, alcohol precipitation staging has more industrialized prospect, but alcohol precipitation staging, in interpolation alcohol process, coprecipitated phenomenon easily occurs, and grading effect is poor, in addition, sharply increases cause dextrin yield low at the later stage system volume of classification.
Therefore, the present invention aims to provide the novel method of classification dextrin, thus provides the novel method reducing dextrin molecular weight distribution.The reverse thinking of team of the present invention: on the basis of alcohol precipitation fractionation, exploitation alcohol progressively dissolves the method for classification dextrin.Namely progressively reduce the content of ethanol in solution, dextrin is progressively dissolved from small to large according to molecular weight, thus realizes the classification of dextrin.
Summary of the invention
The object of this invention is to provide a kind of method of classification dextrin.
Technical scheme of the present invention: a kind of method of classification dextrin, utilize alcohol progressively dissolving technology classification dextrin, technique is:
1, take 10-20g dextrin be placed in 100mL concentration be 80% alcoholic solution at 20-25 DEG C, stir 4-6h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 1 of classification; Filter cake be placed in 100mL concentration be 60% alcoholic solution at 20-25 DEG C, stir 4-6h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 2 of classification; Filter cake be placed in 100mL concentration be 40% alcoholic solution at 20-25 DEG C, stir 4-6h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 3 of classification; Filter cake be placed in 100mL concentration be 20% alcoholic solution at 20-25 DEG C, stir 4-6h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 4 of classification; Filter cake is placed in 100mL distilled water and stirs 4-6h at 20-25 DEG C, centrifugal 10min under 10000g, and supernatant liquor drying obtains the dextrin 5 of classification, and filter cake obtains the dextrin 6 of classification through washing drying, so dextrin is classified into 5-6 dextrin component.
2, dextrin dispersiveness qualification: adopt efficient exclusion chromatography associating multi-angle laser detector and Composition distribution to dextrin proximate analysis, calculate molecular weight and the dispersion coefficient of dextrin.
Described alcohol is methyl alcohol, ethanol or Virahol.
The inventive method principle is larger based on dextrin molecular weight, and solvability is poorer, therefore, progressively increases alcohol content in solution, dextrin can be made progressively to be dissolved by alcoholic solution by molecular weight is ascending, thus realize the classification of dextrin.Compared with prior art, the present invention has the beneficial effects such as production technique is simple, cost is low, yield is high, treatment capacity is large, grading effect is good.
Embodiment
Embodiment 1
(weight-average molecular weight is 1.032 × 10 to take 10g dextrin
4da, dispersion coefficient is 1.819) be placed in 100mL concentration be 80% methanol solution at 20 DEG C, stir 4h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 1 of classification; Filter cake be placed in 100mL concentration be 60% methanol solution at 20 DEG C, stir 4h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 2 of classification; Filter cake be placed in 100mL concentration be 40% methanol solution at 20 DEG C, stir 4h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 3 of classification; Filter cake be placed in 100mL concentration be 20% methanol solution at 20 DEG C, stir 4h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 4 of classification; Filter cake is placed in 100mL distilled water and stirs 4h at 20 DEG C, and now, dextrin dissolves supernatant liquor completely and dissolves the dextrin 5 that drying obtains classification completely.5 dextrin component molecular amount information are as follows: dextrin 1 weight-average molecular weight is 2.130 × 10
3da, dispersion coefficient is 1.018; Dextrin 2 weight-average molecular weight is 4.164 × 10
3da, dispersion coefficient is 1.103; Dextrin 3 weight-average molecular weight is 7.011 × 10
3da, dispersion coefficient is 1.116; Dextrin 4 weight-average molecular weight is 1.329 × 10
4da, dispersion coefficient is 1.131; Dextrin 5 weight-average molecular weight is 1.929 × 10
4da, dispersion coefficient is 1.313.The dextrin specific dispersivity that classification obtains, all lower than parent dextrin, shows that the dextrin molecular weight distribution that classification obtains is narrower than parent dextrin.
Embodiment 2
(weight-average molecular weight is 1.432 × 10 to take 20g dextrin
4da, dispersion coefficient is 2.029) be placed in 100mL concentration be 80% methanol solution at 25 DEG C, stir 6h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 1 of classification; Filter cake be placed in 100mL concentration be 60% methanol solution at 25 DEG C, stir 6h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 2 of classification; Filter cake be placed in 100mL concentration be 40% methanol solution at 25 DEG C, stir 6h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 3 of classification; Filter cake be placed in 100mL concentration be 20% methanol solution at 25 DEG C, stir 6h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 4 of classification; Filter cake is placed in 100mL distilled water and stirs 6h at 25 DEG C; Filter cake is placed in 100mL distilled water and stirs 6h at 25 DEG C, centrifugal 10min under 10000g, and supernatant liquor drying obtains the dextrin 5 of classification, and filter cake obtains the dextrin 6 of classification through washing drying.6 dextrin component molecular amount information are as follows: dextrin 1 weight-average molecular weight is 2.554 × 10
3da, dispersion coefficient is 1.025; Dextrin 2 weight-average molecular weight is 5.023 × 10
3da, dispersion coefficient is 1.121; Dextrin 3 weight-average molecular weight is 7.531 × 10
3da, dispersion coefficient is 1.126; Dextrin 4 weight-average molecular weight is 1.613 × 10
4da, dispersion coefficient is 1.121; Dextrin 5 weight-average molecular weight is 2.345 × 10
4da, dispersion coefficient is 1.398; Dextrin 6 weight-average molecular weight is 3.012 × 10
4da, dispersion coefficient is 1.501.The dextrin specific dispersivity that classification obtains, all lower than parent dextrin, shows that the dextrin molecular weight distribution that classification obtains is narrower than parent dextrin.
Embodiment 3
(weight-average molecular weight is 1.032 × 10 to take 15g dextrin
4da, dispersion coefficient is 1.819) be placed in 100mL concentration be 80% ethanolic soln at 25 DEG C, stir 4h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 1 of classification; Filter cake be placed in 100mL concentration be 60% ethanolic soln at 25 DEG C, stir 4h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 2 of classification; Filter cake be placed in 100mL concentration be 40% ethanolic soln at 25 DEG C, stir 4h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 3 of classification; Filter cake be placed in 100mL concentration be 20% ethanolic soln at 25 DEG C, stir 4h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 4 of classification; Filter cake is placed in 100mL distilled water and stirs 4h at 25 DEG C, centrifugal 10min under 10000g, and supernatant liquor drying obtains the dextrin 5 of classification, and filter cake obtains the dextrin 6 of classification through washing drying.6 dextrin component molecular amount information are as follows: dextrin 1 weight-average molecular weight is 2.851 × 10
3da, dispersion coefficient is 1.039; Dextrin 2 weight-average molecular weight is 4.865 × 10
3da, dispersion coefficient is 1.153; Dextrin 3 weight-average molecular weight is 7.901 × 10
3da, dispersion coefficient is 1.121; Dextrin 4 weight-average molecular weight is 1.421 × 10
4da, dispersion coefficient is 1.205; Dextrin 5 weight-average molecular weight is 2.011 × 10
4da, dispersion coefficient is 1.324; Dextrin 6 weight-average molecular weight is 2.567 × 10
4da, dispersion coefficient is 1.451.The dextrin specific dispersivity that classification obtains, all lower than parent dextrin, shows that the dextrin molecular weight distribution that classification obtains is narrower than parent dextrin.
Embodiment 4
(weight-average molecular weight is 1.432 × 10 to take 20g dextrin
4da, dispersion coefficient is 2.029) be placed in 100mL concentration be 80% aqueous isopropanol at 25 DEG C, stir 6h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 1 of classification; Filter cake be placed in 100mL concentration be 60% aqueous isopropanol at 25 DEG C, stir 6h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 2 of classification; Filter cake be placed in 100mL concentration be 40% aqueous isopropanol at 25 DEG C, stir 6h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 3 of classification; Filter cake be placed in 100mL concentration be 20% aqueous isopropanol at 25 DEG C, stir 6h, centrifugal 10min under 10000g, supernatant liquor drying obtains the dextrin 4 of classification; Filter cake is placed in 100mL distilled water and stirs 6h at 25 DEG C, centrifugal 10min under 10000g, and supernatant liquor drying obtains the dextrin 5 of classification, and filter cake obtains the dextrin 6 of classification through washing drying.6 dextrin component molecular amount information are as follows: dextrin 1 weight-average molecular weight is 3.327 × 10
3da, dispersion coefficient is 1.139; Dextrin 2 weight-average molecular weight is 5.236 × 10
3da, dispersion coefficient is 1.201; Dextrin 3 weight-average molecular weight is 8.756 × 10
3da, dispersion coefficient is 1.235; Dextrin 4 weight-average molecular weight is 1.723 × 10
4da, dispersion coefficient is 1.312; Dextrin 5 weight-average molecular weight is 2.854 × 10
4da, dispersion coefficient is 1.389; Dextrin 6 weight-average molecular weight is 3.415 × 10
4da, dispersion coefficient is 1.310.The dextrin specific dispersivity that classification obtains, all lower than parent dextrin, shows that the dextrin molecular weight distribution that classification obtains is narrower than parent dextrin.
Claims (2)
1. the method for a classification dextrin, it is characterized in that adopting alcohol progressively dissolution method classification dextrin, concrete operations are as follows: take 10-20g dextrin be placed in 100mL concentration be 80% alcoholic solution at 20-25 DEG C, stir 4-6h, centrifugal, supernatant liquor drying obtains the dextrin 1 of classification; Filter cake be placed in 100mL concentration be 60% alcoholic solution at 20-25 DEG C, stir 4-6h, centrifugal, supernatant liquor drying obtains the dextrin 2 of classification; Filter cake be placed in 100mL concentration be 40% alcoholic solution at 20-25 DEG C, stir 4-6h, centrifugal, supernatant liquor drying obtains the dextrin 3 of classification; Filter cake be placed in 100mL concentration be 20% alcoholic solution at 20-25 DEG C, stir 4-6h, centrifugal, supernatant liquor drying obtains the dextrin 4 of classification; Filter cake is placed in 100mL distilled water and stirs 4-6h at 20-25 DEG C, centrifugal, and supernatant liquor drying obtains the dextrin 5 of classification, and filter cake obtains the dextrin 6 of classification through washing drying, so dextrin is classified into 5-6 dextrin component.
2. the method for classification dextrin according to claim 1, is characterized in that: the alcohol of use is methyl alcohol, ethanol or Virahol.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113621084A (en) * | 2021-07-15 | 2021-11-09 | 南昌大学 | Method for grading dextrin |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5225219A (en) * | 1992-01-02 | 1993-07-06 | The United States Of America, As Represented By The Secretary Of Agriculture | Amylodextrin compositions and method therefor |
| CN104198668A (en) * | 2014-09-17 | 2014-12-10 | 江南大学 | Dextrin grading method |
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- 2015-10-23 CN CN201510697479.3A patent/CN105418772B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5225219A (en) * | 1992-01-02 | 1993-07-06 | The United States Of America, As Represented By The Secretary Of Agriculture | Amylodextrin compositions and method therefor |
| CN104198668A (en) * | 2014-09-17 | 2014-12-10 | 江南大学 | Dextrin grading method |
Non-Patent Citations (4)
| Title |
|---|
| BERTOFT E. ET AL: "Fractional preciptation of amylopectin alpha-dextrins using methanol", 《CARBOHYDRATE RESEARCH》 * |
| GELDERS G. ET AL: "Fractionation of starch hydrolysates into dextrins with narrow molecular mass distribution and their detection by high-performance anion-exchange chromatography with pulsed amperometric detection", 《JOURNAL OF CHROMATOGRAPHY A》 * |
| I.DEFLOOR V V ET AL: "Fractionation of maltodextrins by ethanol", 《JOURNAL OF CHROMATOGRAPHY A》 * |
| 胡秀婷: "淀粉醇酸降解制备糊精及糊精的分级与应用研究", 《中国博士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113621084A (en) * | 2021-07-15 | 2021-11-09 | 南昌大学 | Method for grading dextrin |
| CN113621084B (en) * | 2021-07-15 | 2022-10-11 | 南昌大学 | Method for grading dextrin |
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Effective date of registration: 20200422 Address after: 273100 No.1 Industrial Park, Shimenshan Town, Qufu City, Jining City, Shandong Province Patentee after: Qufu bestI biomedical Co., Ltd Address before: 999 No. 330031 Jiangxi province Nanchang Honggutan University Avenue Patentee before: Lattice Power (Jiangxi) Corp. |
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