CN105417492A - Method for preparing beta-cyclodextrin-gold nano-particles and method for detecting cholesterol - Google Patents
Method for preparing beta-cyclodextrin-gold nano-particles and method for detecting cholesterol Download PDFInfo
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Abstract
The invention discloses a method for preparing beta-cyclodextrin-gold nano-particles and a method for detecting cholesterol. The method for preparing the beta-cyclodextrin-gold nano-particles includes enabling water, gold source solution and beta-cyclodextrin solution to carry out contact reaction in the presence of buffer solution to obtain the beta-cyclodextrin-gold nano-particles. The gold source solution can be one or a plurality of types of chloroauric acid trihydrate solution, chloroaurate solution, tetra-nitroxyl alloy acid solution and tetra-nitroxyl alloy salt solution. The methods have the advantages that the beta-cyclodextrin-gold nano-particles can be prepared at one step, raw materials of the beta-cyclodextrin-gold nano-particles are easily available, and post-treatment is simple; the cholesterol can be sensitively and quantitatively detected by the aid of the beta-cyclodextrin-gold nano-particles, and the beta-cyclodextrin-gold nano-particles are excellent in anti-interference capacity.
Description
Technical field
The present invention relates to beta-schardinger dextrin--golden nanometer particle, particularly, relate to a kind of preparation method of beta-schardinger dextrin--golden nanometer particle and the detection method of cholesterol.
Background technology
Diameter is at nano level nano Au particle, its elementary cell is all minute sized particle, therefore the physical characteristic had not available for a lot of macroparticle, as optical effect, small-size effect, skin effect, macro quanta tunnel effect, Dielectric confinement effect, Kub o effect and some other special effects.These effects make nano Au particle be widely used in each fields such as material, medical test, clinical medicine, food, chemical industry, pottery, dyestuff.
Golden nanometer particle (namely by golden nanometer particle that macromolecular substances is modified) with supramolecular structure has had the characteristic of golden nanometer particle and macromolecular substances concurrently, uses all widely at present in science and technology field.With the golden nanometer particle of beta-cyclodextrin modified, current synthetic method mainly contains two classes: the first kind is two steps or multistep processes synthesis, namely needs first to synthesize golden nanometer particle, then by beta-cyclodextrin modified on golden nanometer particle surface; Equations of The Second Kind is one-step synthesis method method, but adds highly basic (as NaOH) or strong reductant in building-up process (as NaBH
4).Although above-mentioned two class methods can synthesize the golden nanometer particle of beta-cyclodextrin modified, otherwise comparatively loaded down with trivial details, or be need adding assistant and then cause post processing numerous and diverse, thus significantly limit the application of the golden nanometer particle of beta-cyclodextrin modified.
Summary of the invention
The object of this invention is to provide a kind of preparation method of beta-schardinger dextrin--golden nanometer particle and the detection method of cholesterol, the method a step can obtain beta-schardinger dextrin--golden nanometer particle and raw material is easy to get, post processing is simple; Utilize this beta-schardinger dextrin--golden nanometer particle quantitatively to detect cholesterol delicately simultaneously and there is excellent antijamming capability.
To achieve these goals, the invention provides a kind of preparation method of beta-schardinger dextrin--golden nanometer particle, this preparation method is: under the existence of cushioning liquid, and Jiang Shui, golden source solution and beta-schardinger dextrin-solution carry out haptoreaction and obtains beta-schardinger dextrin--golden nanometer particle; Wherein, golden source solution is selected from one or more in three hydration chlorauric acid solutions, chloroaurate solution, four nitrato alloy acid solutions and four nitrato alloy salts solution.
Present invention also offers a kind of detection method of cholesterol, comprising:
1) above-mentioned beta-schardinger dextrin--golden nanometer particle is carried out disperseing obtained beta-schardinger dextrin--solution of gold nanoparticles in dispersant;
2) rhodamine B solution and beta-schardinger dextrin--solution of gold nanoparticles are mixed and made into solvent, then in solvent, add the cholesterol solution of variable concentrations and with water constant volume to make solution to be detected, then detect to record fluorescence radiation intensity by fluorescence spectrophotometry;
3) with the concentration of cholesterol solution for abscissa, fluorescence radiation intensity is that ordinate obtains calibration curve and/or calibration curve equation;
4) cholesterol solution of unknown concentration is added in solvent, then carries out fluorescence spectrophotometry and detect to obtain fluorescence radiation intensity, then calculated the concentration of the cholesterol solution of unknown concentration by calibration curve and/or calibration curve equation.
Pass through technique scheme, in the present invention, the preparation method of beta-schardinger dextrin--golden nanometer particle adopts one-step method to synthesize, the golden nanometer particle with cavity structure that utilized the reproducibility of beta-schardinger dextrin-itself to obtain fully, as shown in Fig. 1 (wherein AuNP is golden nanometer particle), beta-schardinger dextrin--golden nanometer particle is that the coated golden nanometer particle of beta-schardinger dextrin-forms, and then avoids using highly basic (as NaOH) or strong reductant (as NaBH
4) to simplify the purification process of post processing.And, the present invention utilizes obtained beta-schardinger dextrin--golden nanometer particle to carry out quantitative detection to cholesterol solution, principle is see Fig. 2, in the cavity of i.e. cholesterol and then beta-schardinger dextrin--golden nanometer particle, and then excite generation fluorescence, and then by fluorescence scene photometry, cholesterol solution is quantitatively detected; This detection method is not only highly sensitive, and simultaneously repeatability is good and have the antijamming capability of excellence.
Other features and advantages of the present invention are described in detail in detailed description of the invention part subsequently.
Accompanying drawing explanation
Accompanying drawing is used to provide a further understanding of the present invention, and forms a part for description, is used from explanation the present invention, but is not construed as limiting the invention with detailed description of the invention one below.In the accompanying drawings:
Fig. 1 is the preparation principle figure of beta-schardinger dextrin--golden nanometer particle in embodiment 1;
Fig. 2 is the Cleaning Principle figure of cholesterol in application examples 1;
Fig. 3 is the ultraviolet spectrogram of A1 in test example 1;
Fig. 4 is the transmission electron microscope picture of the amplification 8,000,000 times of A1 in test example 2;
Fig. 5 is the scanning electron microscope (SEM) photograph of the amplification 100,000 times of A1 in test example 3;
Fig. 6 is the infrared spectrogram of A1 in test example 4;
Fig. 7 is the XPS oxygen spectrogram of beta-schardinger dextrin-;
Fig. 8 is the XPS oxygen spectrogram of A1 in test example 5;
Fig. 9 is the XPS carbon spectrogram of beta-schardinger dextrin-;
Figure 10 is the XPS carbon spectrogram of A1 in test example 5;
Figure 11 is the nucleus magnetic hydrogen spectrum figure of A1 in test example 6;
Figure 12 is fluorescence spectrum figure in test example 7;
Figure 13 is the result statistical chart of maximum fluorescence luminous intensity in Figure 12;
Figure 14 is fluorescence spectrum figure in application examples 1;
Figure 15 is the result statistical chart of maximum fluorescence luminous intensity in Figure 14;
Figure 16 is the result statistical chart of the anti-interference detection of A1 in application examples 2.
Detailed description of the invention
Below the specific embodiment of the present invention is described in detail.Should be understood that, detailed description of the invention described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
The invention provides a kind of preparation method of beta-schardinger dextrin--golden nanometer particle, this preparation method is: under the existence of cushioning liquid, and Jiang Shui, golden source solution and beta-schardinger dextrin-solution carry out haptoreaction and obtains beta-schardinger dextrin--golden nanometer particle; Wherein, golden source solution is selected from one or more in three hydration chlorauric acid solutions, chloroaurate solution, four nitrato alloy acid solutions and four nitrato alloy salts solution.
In the above-mentioned methods, the concrete kind of cushioning liquid and pH can select in wide scope, but as much as possible golden nanometer particle is modified to make the beta-schardinger dextrin-obtained, taking cost into account simultaneously, preferably, the pH of cushioning liquid is 6.8-7.5, and cushioning liquid is selected from the one in phosphate buffer solution, hac buffer, trishydroxymethylaminomethane hydrochloric acid buffer solution and disodium bicarbonate citric acid solution.
In the above-mentioned methods, the concrete kind of chloroaurate solution and four nitrato alloy salts solution can be selected in wide scope, but in order to taking cost into account, preferably, chloroaurate solution is sodium chloraurate solution and/or potassium chloroaurate solution; Four nitrato alloy salts solution are four nitrato alloy potassium solutions and/or four nitrato alloy sodium solutions.
In the present invention, the consumption of each material and concentration can be selected in wide scope, in order to improve the productive rate of obtained beta-schardinger dextrin--golden nanometer particle, preferably, the concentration of gold source solution is 0.009-0.012mol/L, and the concentration of beta-schardinger dextrin-solution is 0.009-0.015mol/L; Further, relative to the golden source solution of 1ml, the consumption of beta-schardinger dextrin-solution is 8-11ml, and the consumption of water is 30-40ml, and the consumption of cushioning liquid is 4.5-5.5ml.
In the present invention, catalytic actual conditions can be selected in wide scope, in order to improve the productive rate of obtained beta-schardinger dextrin--golden nanometer particle, preferably, haptoreaction at least meets the following conditions: reaction temperature is 90-105 DEG C, and the reaction end time controls after reaction system becomes claret; And haptoreaction is carry out under stirring condition.
In the present invention, the method of purification of beta-schardinger dextrin--golden nanometer particle can be the post-processing approach of any one routine in this area, but consider from the convenience of operation, preferably, haptoreaction also comprises: after reaction system becomes claret, reaction system is carried out centrifugal treating, then gets lower sediment.More preferably, centrifugal treating at least meets the following conditions: centrifugal rotational speed is 5500-8000rpm, and centrifugation time is 5-10min.
Present invention also offers a kind of detection method of cholesterol, comprising:
1) above-mentioned beta-schardinger dextrin--golden nanometer particle is carried out disperseing obtained beta-schardinger dextrin--solution of gold nanoparticles in dispersant;
2) rhodamine B solution and beta-schardinger dextrin--solution of gold nanoparticles are mixed and made into solvent, then in solvent, add the cholesterol solution of variable concentrations and with water constant volume to make solution to be detected, then detect to record fluorescence radiation intensity by fluorescence spectrophotometry;
3) with the concentration of cholesterol solution for abscissa, fluorescence radiation intensity is that ordinate obtains calibration curve and/or calibration curve equation;
4) cholesterol solution of unknown concentration is added in solvent, then carries out fluorescence spectrophotometry and detect to obtain fluorescence radiation intensity, then calculated the concentration of the cholesterol solution of unknown concentration by calibration curve and/or calibration curve equation.
In the detection method of above-mentioned cholesterol, the kind of dispersant and the concentration of beta-schardinger dextrin--solution of gold nanoparticles can be selected in wide scope, in order to obtain more stable testing result, preferably, in step 1) in, dispersant is water; And the concentration of beta-schardinger dextrin--solution of gold nanoparticles is 4.8 × 10
-9-6.12 × 10
-9mol/L.
In the detection method of above-mentioned cholesterol, the concentration detecting each material in solution can be selected in wide scope, in order to obtain more stable testing result, in step 2) in, in detection solution, the concentration of rhodamine B is 0.038-0.042 μM, and the concentration of beta-schardinger dextrin--golden nanometer particle is not less than 0.4nM.
In the detection method of above-mentioned cholesterol, in order to obtain more stable testing result, preferably, in fluorescence spectrophotometry detects, blank solution is solvent; Determined wavelength is 550-750nm.
Below will be described the present invention by embodiment.
Embodiment 1
1) the 0.1mol/L phosphate buffer solution (pH=7) of 5mL will be added in 35mL ultra-pure water, then the beta-schardinger dextrin-solution adding the three hydration chlorauric acid solutions of the 0.01mol/L of 1mL and the 0.01mol/L of 10mL 100 DEG C of strong agitation, until reaction system becomes claret gradually;
2) by above-mentioned reaction system in rotating speed be 6000rpm condition under carry out centrifugal treating 5min, then get lower sediment and namely obtain beta-schardinger dextrin--golden nanometer particle A1.
Embodiment 2
Obtained beta-schardinger dextrin--golden nanometer particle A2 is carried out according to the method for embodiment 1, unlike, the consumption of beta-schardinger dextrin-is 2mL.
Embodiment 3
Obtained beta-schardinger dextrin--golden nanometer particle A3 is carried out according to the method for embodiment 1, unlike, the consumption of beta-schardinger dextrin-is 10mL.
Embodiment 4
Obtained beta-schardinger dextrin--golden nanometer particle A5 is carried out according to the method for embodiment 1, unlike, the pH of phosphate buffer solution is 7.2, and consumption is 5ml.
Embodiment 5
Obtained beta-schardinger dextrin--golden nanometer particle A5 is carried out according to the method for embodiment 1, unlike, the pH of phosphate buffer solution is 6.9, and consumption is 5ml.
Embodiment 6
Obtained beta-schardinger dextrin--golden nanometer particle A6 is carried out according to the method for embodiment 1, unlike, the consumption of water is 40mL.
Embodiment 7
Obtained beta-schardinger dextrin--golden nanometer particle A7 is carried out according to the method for embodiment 1, unlike, the consumption of water is 30mL.
Embodiment 8
Obtained beta-schardinger dextrin--golden nanometer particle A8 is carried out according to the method for embodiment 1, unlike, reaction temperature is 105 DEG C.
Embodiment 9
Obtained beta-schardinger dextrin--golden nanometer particle A9 is carried out according to the method for embodiment 1, unlike, reaction temperature is 92 DEG C.
Test example 1
Be that the ultraviolet spectrometer of UV-2910 carries out ultraviolet spectra detection to beta-schardinger dextrin--golden nanometer particle A1 by the trade mark, testing result is shown in Fig. 3, and the surface plasma absworption peak of beta-schardinger dextrin-as shown in Figure 3-golden nanometer particle A1 is 520nm.In like manner detect and learn, the surface plasma absworption peak of A2-A9 is also near 520nm.Due to the characteristic absorption peak that 520nm is golden nanometer particle, thus directly demonstrate in A1-A9 containing golden nanometer particle.
Test example 2
Be that the transmission electron microscope of TecnaiG220ST (FEI) carries out transmission electron microscope detection to beta-schardinger dextrin--golden nanometer particle A1 by the trade mark, testing result is shown in Fig. 4, beta-schardinger dextrin--golden nanometer particle has crystal face 0.233 (111) and 0.203 (200) as shown in Figure 4, the peculiar crystal face of this golden nanometer particle, demonstrate containing golden nanometer particle in A1 further, in like manner detect and learn in A2-A9 also containing golden nanometer particle.
Test example 3
Be that the SEM of HitachiS-4800 carries out ESEM detection to beta-schardinger dextrin--golden nanometer particle A1 by the trade mark, testing result is shown in Fig. 5, the particle diameter of beta-schardinger dextrin--golden nanometer particle is 17-22nm as shown in Figure 5, and in like manner detecting the particle diameter learning A2-A9 is also 17-22nm.
Test example 4
Be that the infrared spectrometer of PerkinElmerPE-983 carries out infrared spectrum detection to beta-schardinger dextrin--golden nanometer particle A1 by the trade mark, testing result is shown in Fig. 6, as shown in Figure 6, A1 has beta-schardinger dextrin-, but there is again obvious difference with beta-schardinger dextrin-simultaneously, indirectly proved further in A1 containing golden nanometer particle.In like manner, the row infrared spectrum testing result of A2-A9 and Fig. 6 are consistent.
Test example 5
By the trade mark be ESCALAB250XiXPS X-ray photoelectron spectroscopic analysis to beta-schardinger dextrin--golden nanometer particle A1 carry out XPS oxygen spectrum and XPS carbon spectrum detect, XPS oxygen spectrogram is shown in Fig. 8, XPS carbon spectrogram is shown in Figure 10, and in addition, Fig. 7 and Fig. 8 is followed successively by XPS oxygen spectrogram and the XPS carbon spectrogram of beta-schardinger dextrin-.Carboxyl has been become, by carboxyl and golden coordination from the OH in the surface cavities of the beta-schardinger dextrin-of Fig. 7-10, A1.In like manner, the XPS testing result of A2-A9 and the XPS testing result of A1 are consistent.
Test example 6
Be that the NMR of BrukerAvance-300 carries out nucleus magnetic hydrogen spectrum detection to beta-schardinger dextrin--golden nanometer particle A1 by the trade mark, testing result is shown in Figure 11 and table 1, the beta-schardinger dextrin-(β-CD) on A1 surface is complete cavity structure, and namely A1 is formed by the coated golden nanometer particle of beta-schardinger dextrin-(AuNPs represents multiple golden nanometer particle).In like manner, the testing result of the testing result of the nucleus magnetic hydrogen spectrum of A2-A9 and the nucleus magnetic hydrogen spectrum of A1 is consistent.
Table 1
| H1 | H2 | H3 | H4 | H5 | H6 | |
| β-CD | 4.989 | 3.568 | 3.888 | 3.503 | 3.778 | 3.803 |
| AuNP | 4.910 | 3.510 | 3.830 | 3.504 | 3.774 | 3.805 |
Test example 7
Be that 8:1 disperses to make concentration by beta-schardinger dextrin--golden nanometer particle A1 and water according to volume ratio be 4.836 × 10
-9then 2 μMs of rhodamine B solution of 60 μ L are mixed with the beta-schardinger dextrin--solution of gold nanoparticles of different volumes, are finally settled to 3ml and place 10min by the beta-schardinger dextrin--solution of gold nanoparticles of mol/L.
Detected the fluorescence intensity (determined wavelength is 550-750nm) of above-mentioned solution by fluorescence spectrophotometry, the results are shown in Figure 12 and 13, wherein Figure 13 is the result statistical chart of maximum fluorescence luminous intensity in Figure 12; It can thus be appreciated that the Fluorescence-quenching that golden nanometer particle concentration is greater than the solution of 0.4nM is the most obvious.In like manner, the testing result of the testing result of the nucleus magnetic hydrogen spectrum of A2-A9 and the nucleus magnetic hydrogen spectrum of A1 is consistent.
Application examples 1
Be that 8:1 disperses to make concentration by beta-schardinger dextrin--golden nanometer particle A1 and water according to volume ratio be 4.836 × 10
-9beta-schardinger dextrin--the solution of gold nanoparticles of mol/Lmol/L, then 2 μMs of rhodamine B solution of 60 μ L are mixed with the beta-schardinger dextrin--solution of gold nanoparticles of 250 μ L, then the cholesterol solution of different volumes is added, finally be settled to 3ml and place 10min (wherein, the concentration of rhodamine B is 0.04 μM, and the concentration of beta-schardinger dextrin--golden nanometer particle is 0.403nM).
Detected the fluorescence intensity (determined wavelength is 550-750nm) of above-mentioned solution by fluorescence spectrophotometry, the results are shown in Figure 14 and 15, wherein Figure 15 is the result statistical chart of maximum fluorescence luminous intensity in Figure 14; It can thus be appreciated that in the scope that cholesterol concentration is 0-4 μM, fluorescence radiation intensity and cholesterol concentration linearly change, and just can make calibration curve equation thus.So, the cholesterol solution of unknown concentration detects that its fluorescence radiation intensity just calculates corresponding concentration by the calibration curve in calibration curve equation or Figure 15.
Application examples 2
Get a series of cuvette, at 25 DEG C, add beta-schardinger dextrin--golden nanometer particle A1 respectively and rhodamine B makes solvent, then the cholesterol that normal human serum dilutes 1000 times amount is added respectively, glutathione, cysteine, and the alanine of normal serum content, arginine, asparatate, glutamic acid, histidine, leucine, lysine, methionine, phenylalanine, proline, serine, tryptophan, tyrosine, valine, ascorbic acid, glycine, threonine, dopamine, human albumin and above-mentioned mixing, then add water and be settled to 3mL, fluorescence intensity (taking solvent as blank group) after placement 10min, fluorescence data result is shown in Figure 16, wherein, F represents the fluorescence intensity of above-mentioned various material, F
grepresent the fluorescence intensity of blank, F
0represent that fluorescence intensity Figure 16 of beta-schardinger dextrin--golden nanometer particle and rhodamine B solvent is known, by A1 and rhodamine B cholesterol detection, there is excellent interference free performance.In like manner, by A2-A9 cholesterol detection, also there is excellent anti-interference.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characteristic described in above-mentioned detailed description of the invention, in reconcilable situation, can be combined by any suitable mode, in order to avoid unnecessary repetition, the present invention illustrates no longer separately to various possible combination.
In addition, also can be combined between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (10)
1. a preparation method for beta-schardinger dextrin--golden nanometer particle, is characterized in that, described preparation method is: under the existence of cushioning liquid, and Jiang Shui, golden source solution and beta-schardinger dextrin-solution carry out haptoreaction and obtains described beta-schardinger dextrin--golden nanometer particle; Wherein, described golden source solution is selected from one or more in three hydration chlorauric acid solutions, chloroaurate solution, four nitrato alloy acid solutions and four nitrato alloy salts solution.
2. preparation method according to claim 1, wherein, the pH of described cushioning liquid is 6.8-7.5, and described cushioning liquid is selected from the one in phosphate buffer solution, hac buffer, trishydroxymethylaminomethane hydrochloric acid buffer solution and disodium bicarbonate citric acid solution.
3. preparation method according to claim 1, wherein, described chloroaurate solution is sodium chloraurate solution and/or potassium chloroaurate solution; Described four nitrato alloy salts solution are four nitrato alloy potassium solutions and/or four nitrato alloy sodium solutions.
4. according to the preparation method in claim 1-3 described in any one, wherein, the concentration of described golden source solution is 0.009-0.012mol/L, and the concentration of described beta-schardinger dextrin-solution is 0.009-0.015mol/L; Further, relative to the described golden source solution of 1ml, the consumption of described beta-schardinger dextrin-solution is 8-11ml, and the consumption of described water is 30-40ml, and the consumption of described cushioning liquid is 4.5-5.5ml.
5. preparation method according to claim 4, wherein, described haptoreaction at least meets the following conditions: reaction temperature is 90-105 DEG C, and the reaction end time controls after reaction system becomes claret; And described haptoreaction is carry out under stirring condition.
6. according to the preparation method in claim 1-3 and 5 described in any one, wherein, described haptoreaction also comprises: after described reaction system becomes claret, described reaction system is carried out centrifugal treating, then gets lower sediment.
7. preparation method according to claim 6, wherein, described centrifugal treating at least meets the following conditions: centrifugal rotational speed is 5500-8000rpm, and centrifugation time is 5-10min.
8. a detection method for cholesterol, is characterized in that, comprising:
1) beta-schardinger dextrin--golden nanometer particle in claim 1-7 described in any one is carried out disperseing obtained beta-schardinger dextrin--solution of gold nanoparticles in dispersant;
2) rhodamine B solution and beta-schardinger dextrin--solution of gold nanoparticles are mixed and made into solvent, then in described solvent, add the cholesterol solution of variable concentrations and with water constant volume to make solution to be detected, then detect to record fluorescence radiation intensity by fluorescence spectrophotometry;
3) with the concentration of described cholesterol solution for abscissa, fluorescence radiation intensity is that ordinate obtains calibration curve and/or calibration curve equation;
4) cholesterol solution of unknown concentration is added in described solvent, then carry out fluorescence spectrophotometry to detect to obtain fluorescence radiation intensity, then calculated the concentration of the cholesterol solution of described unknown concentration by described calibration curve and/or calibration curve equation.
9. preparation method according to claim 8, wherein, in step 1) in, described dispersant is water; And the concentration of described beta-schardinger dextrin--solution of gold nanoparticles is 4.8 × 10
-9-6.12 × 10
-9mol/L;
In step 2) in, in described detection solution, the concentration of rhodamine B is 0.038-0.042 μM, and the concentration of described beta-schardinger dextrin--golden nanometer particle is not less than 0.4nM.
10. preparation method according to claim 8 or claim 9, wherein, in described fluorescence spectrophotometry detects, blank solution is described solvent; Determined wavelength is 550-750nm.
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| CN109520979A (en) * | 2018-11-02 | 2019-03-26 | 闽南师范大学 | A kind of detection method of cholesterol in serum |
| CN113801650A (en) * | 2021-08-26 | 2021-12-17 | 桂林电子科技大学 | Sulfydryl beta-cyclodextrin-gold nanocluster and preparation method and application thereof |
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| CN107556999A (en) * | 2017-07-28 | 2018-01-09 | 安徽师范大学 | Gold nano cluster and its preparation method and application |
| CN109142312A (en) * | 2018-07-23 | 2019-01-04 | 华东师范大学 | A kind of plasticiser neck phthalic acid ester detection method based on Surface enhanced Raman spectroscopy |
| CN109014240A (en) * | 2018-08-09 | 2018-12-18 | 华东理工大学 | The preparation method of the gold nano cluster of energy efficient transmission short wavelength-NIR light |
| CN109520979A (en) * | 2018-11-02 | 2019-03-26 | 闽南师范大学 | A kind of detection method of cholesterol in serum |
| CN109520979B (en) * | 2018-11-02 | 2021-07-20 | 闽南师范大学 | Method for detecting cholesterol in serum |
| CN113801650A (en) * | 2021-08-26 | 2021-12-17 | 桂林电子科技大学 | Sulfydryl beta-cyclodextrin-gold nanocluster and preparation method and application thereof |
| CN113801650B (en) * | 2021-08-26 | 2023-07-14 | 桂林电子科技大学 | Sulfhydryl beta-cyclodextrin-gold nanocluster and preparation method and application thereof |
| CN114767871A (en) * | 2022-04-19 | 2022-07-22 | 中国工程物理研究院机械制造工艺研究所 | Mesoporous silicon drug-loaded system, preparation method thereof and mesoporous silicon drug-loaded system |
| CN114767871B (en) * | 2022-04-19 | 2023-04-07 | 中国工程物理研究院机械制造工艺研究所 | Mesoporous silicon drug-loaded system, preparation method thereof and mesoporous silicon drug-loaded system |
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