CN105395564A - 3’,6-二芥子酰基蔗糖酯的抗氧化活性用途 - Google Patents

3’,6-二芥子酰基蔗糖酯的抗氧化活性用途 Download PDF

Info

Publication number
CN105395564A
CN105395564A CN201510417777.2A CN201510417777A CN105395564A CN 105395564 A CN105395564 A CN 105395564A CN 201510417777 A CN201510417777 A CN 201510417777A CN 105395564 A CN105395564 A CN 105395564A
Authority
CN
China
Prior art keywords
disinapoylsucrose
ester
disinapoyl
sucrose
value
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510417777.2A
Other languages
English (en)
Inventor
栾连军
施琦渊
吴永江
刘雪松
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang University ZJU
Original Assignee
Zhejiang University ZJU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang University ZJU filed Critical Zhejiang University ZJU
Priority to CN201510417777.2A priority Critical patent/CN105395564A/zh
Publication of CN105395564A publication Critical patent/CN105395564A/zh
Pending legal-status Critical Current

Links

Landscapes

  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Cosmetics (AREA)

Abstract

本发明提供一种3’,6-二芥子酰基蔗糖酯在制备抗氧化活性的药物或化妆品中的应用。本发明通过研究发现：3’,6-二芥子酰基蔗糖酯对DPPH自由基清除的IC50值为1.08mg/mL，阳性对照Vc的IC50值为0.29mg/mL；3’,6-二芥子酰基蔗糖酯对ABTS自由基清除的IC50值为0.33mg/mL，阳性对照Vc的IC50值为0.12mg/mL。因此，3’,6-二芥子酰基蔗糖酯具有较强的抗氧化活性，可以用于制备抗氧化活性的药物或化妆品。

Description

3’,6-二芥子酰基蔗糖酯的抗氧化活性用途
技术领域
本发明涉及医药领域，涉及3’,6-二芥子酰基蔗糖酯在制备抗氧化活性的药物或化妆品中的应用。
背景技术
科学研究表明，自由基或氧化剂会将细胞和组织分解，影响代谢功能，并会引起不同的健康问题。如果能够消除过多的氧化自由基，对于许多自由基引起的及老化相关疾病都能够预防。例如常见的癌症、动脉硬化、糖尿病、白内障、心血管病、老年痴呆、关节炎等，这些疾病都被认为与自由基相关。所以，抗氧化也是市场最重要的功能性诉求之一，新型抗氧化剂的开发被保健品、化妆品企业列为主要的研发方向之一。
3’,6-二芥子酰基蔗糖酯（3’,6-disinapoylsucrose,DISS）是一种酰化寡糖，分子式：C34H42O19，分子量：754.23，淡黄色无定形粉末。分子结构式如下：
3’,6-二芥子酰基蔗糖酯最早是由Linscheid等从萝卜中分离得到的（LinscheidM.,WendischD.,StrackD.ThestructuresofsinapicacidestersandtheirmetabolismincotyledonsofRaphanussativus[J].Z.Naturforsch.,1980,35c:907-914）。到目前为止，未见关于此化合物具有抗氧化活性的报道。
发明内容
本发明的目的是提供3’,6-二芥子酰基蔗糖酯在制备抗氧化活性的药物或化妆品中的应用。所述药物或化妆品中包含作为活性成分的3’,6-二芥子酰基蔗糖酯和助剂。
本发明通过研究发现：3’,6-二芥子酰基蔗糖酯对DPPH自由基清除的IC50值为1.08mg/mL，阳性对照Vc的IC50值为0.29mg/mL；3’,6-二芥子酰基蔗糖酯对ABTS自由基清除的IC50值为0.33mg/mL，阳性对照Vc的IC50值为0.12mg/mL。由此可见，3’,6-二芥子酰基蔗糖酯具有抗氧化活性，是一种天然的抗氧化剂，对人体无副作用，可以用于制备抗氧化活性的药物或化妆品，在该领域具有广阔的应用前景。
附图说明
图1是DPPH法测定3’,6-二芥子酰基蔗糖酯的抗氧化活性（实施例1）。
图2是DPPH法测定维生素C的抗氧化活性（实施例1）。
图3是ABTS法测定3’,6-二芥子酰基蔗糖酯的抗氧化活性（实施例2）。
图4是ABTS法测定维生素C的抗氧化活性（实施例2）。
图5是3’,6-二芥子酰基蔗糖酯和维生素C的总还原能力测定（实施例3）。
具体实施方式
本发明结合附图和实施例作进一步的说明。以下实施例是对本发明的进一步说明，而不是对发明的限制。
实施例1DPPH法测定3’,6-二芥子酰基蔗糖酯的抗氧化活性
取3’,6-二芥子酰基蔗糖酯和维生素C适量，分别用蒸馏水配制一系列浓度供试品溶液。取50μL供试品溶液，加入到3mL的DPPH的甲醇溶液（40μg/mL）中，摇匀，室温下避光静置60min后，在517nm处，测其吸光度（Asample），同时测定50μL蒸馏水与3mL的DPPH溶液混合后的吸光度（Acontrol），以及50μL供试品溶液与3mL甲醇混合后的吸光度（Ablank），计算DPPH清除率（%），并计算IC50值，实验结果见图1，2。DPPH清除率（%）=[1-(Asample-Ablank)/Acontrol]×100%。
研究结果表明：3’,6-二芥子酰基蔗糖酯对DPPH自由基清除的IC50值为1.08mg/mL，阳性对照Vc的IC50值为0.29mg/mL，3’,6-二芥子酰基蔗糖酯清除自由基能力略弱于Vc。
实施例2ABTS法测定3’,6-二芥子酰基蔗糖酯的抗氧化活性
取ABTS1.92g（终浓度为7mM），加入高锰酸钾0.19g（终浓度为2.45mM），至于500mL容量瓶中，加蒸馏水至刻度，摇匀，避光反应16小时，离心去上清液。取适量，加蒸馏水稀释至731nm波长下吸光度为0.7（±0.02），即得ABTS溶液。取3’,6-二芥子酰基蔗糖酯和维生素C适量，分别用蒸馏水配制一系列浓度供试品溶液，取50μL供试品溶液，加入到3mL的ABTS溶液中，摇匀，室温下避光静置70min后测其在731nm下的吸光度（Asample），同时测定50μL蒸馏水与3mL的ABTS溶液混合后的吸光度（Acontrol），计算DPPH清除率（%），并计算IC50值，实验结果见图3，4。DPPH清除率（%）=[(Acontrol-Asample)/Acontrol]×100%
研究结果表明：3’,6-二芥子酰基蔗糖酯对ABTS自由基清除的IC50值为0.33mg/mL，阳性对照Vc的IC50值为0.12mg/mL，3’,6-二芥子酰基蔗糖酯清除自由基能力略弱于Vc。
实施例3
取3’,6-二芥子酰基蔗糖酯和维生素C适量，分别用蒸馏水配制一系列浓度供试品溶液。分别移取1mL不同浓度对照品和供试品溶液，加入2.5mL磷酸钠缓冲盐溶液（0.2M，pH为6.6）和2.5mL铁氰化钾（1%），在50℃反应20min，加入2.5mL三氯乙酸（10%），3000rpm离心10min，取上清液2.5mL，加入2.5mL蒸馏水，加入0.5mL氯化铁（0.1%），700nm测定吸光度，实验结果如图5所示。实验结果表明：3’,6-二芥子酰基蔗糖酯的总还原能力略弱于Vc。
由此可见，本发明的3’,6-二芥子酰基蔗糖酯具有抗氧化活性，可以用于制备抗氧化活性的药物或化妆品。
实施例4
具有抗氧化的保湿柔肤水包括以下组分：丁二醇3g、甘油2g、透明质酸0.1g、卡波姆0.02g、尿囊素0.5g、泛醇0.5g、尼泊金甲酯0.2g、3’,6-二芥子酰基蔗糖1g、香精0.1g、去离子水92.58g。
制作方法：（1）将丁二醇、甘油、透明质酸、卡波姆、尿囊素、泛醇、尼泊金甲酯、3’,6-二芥子酰基蔗糖、去离子水在水相锅中加热至80℃溶解混合均匀；(2）匀质后，于40℃左右加入香精，搅拌均匀；（5）冷却至室温，即得。

Claims (2)

1.一种3’,6-二芥子酰基蔗糖酯在制备抗氧化活性的药物或化妆品中的应用，所述3’,6-二芥子酰基蔗糖酯的结构式为：
。
2.根据权利要求1所述的应用，其特征在于，所述药物或化妆品由3’,6-二芥子酰基蔗糖酯和助剂制成。
CN201510417777.2A 2015-07-16 2015-07-16 3’,6-二芥子酰基蔗糖酯的抗氧化活性用途 Pending CN105395564A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510417777.2A CN105395564A (zh) 2015-07-16 2015-07-16 3’,6-二芥子酰基蔗糖酯的抗氧化活性用途

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510417777.2A CN105395564A (zh) 2015-07-16 2015-07-16 3’,6-二芥子酰基蔗糖酯的抗氧化活性用途

Publications (1)

Publication Number Publication Date
CN105395564A true CN105395564A (zh) 2016-03-16

Family

ID=55461562

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510417777.2A Pending CN105395564A (zh) 2015-07-16 2015-07-16 3’,6-二芥子酰基蔗糖酯的抗氧化活性用途

Country Status (1)

Country Link
CN (1) CN105395564A (zh)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103265584A (zh) * 2013-05-21 2013-08-28 浙江大学 一种从远志中制备3,6’-二芥子酰基蔗糖酯的方法
CN105193830A (zh) * 2015-05-02 2015-12-30 浙江大学 3’,6-二芥子酰基蔗糖酯的抗氧化活性用途

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103265584A (zh) * 2013-05-21 2013-08-28 浙江大学 一种从远志中制备3,6’-二芥子酰基蔗糖酯的方法
CN105193830A (zh) * 2015-05-02 2015-12-30 浙江大学 3’,6-二芥子酰基蔗糖酯的抗氧化活性用途

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YOSHIAKI TAKAYA等: "Antioxidant Constituents of Radish Sprout (Kaiware-daikon),Raphanus sativus L.", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 *

Similar Documents

Publication Publication Date Title
Krasnovskaya et al. Copper coordination compounds as biologically active agents
Kim et al. Skin protective effect of epigallocatechin gallate
Kinney et al. Overall skin health potential of the biofield energy healing based herbomineral formulation using various skin parameters
Wang et al. Free radical scavenging and immunomodulatory activities of Ganoderma lucidum polysaccharides derivatives
Vertuani et al. Activity and stability studies of verbascoside, a novel antioxidant, in dermo-cosmetic and pharmaceutical topical formulations
Freitas et al. Highlighting the biological potential of the brown seaweed Fucus spiralis for skin applications
Gao et al. Antioxidant activity evaluation of dietary flavonoid hyperoside using saccharomyces cerevisiae as a model
Gold-Smith et al. Mangiferin and cancer: Mechanisms of action
Zhu et al. Selenium-containing phycocyanin from Se-enriched Spirulina platensis reduces inflammation in dextran sulfate sodium-induced colitis by inhibiting NF-κB activation
Li et al. Physicochemical characterization and functional analysis of the polysaccharide from the edible microalga Nostoc sphaeroides
Scalia et al. Comparative evaluation of different co-antioxidants on the photochemical-and functional-stability of epigallocatechin-3-gallate in topical creams exposed to simulated sunlight
Wong et al. Tocotrienols reverse cardiovascular, metabolic and liver changes in high carbohydrate, high fat diet-fed rats
Sangthong et al. Polysaccharides from Volvariella volvacea mushroom: Extraction, biological activities and cosmetic efficacy
Wu et al. Protonation of epigallocatechin-3-gallate (EGCG) results in massive aggregation and reduced oral bioavailability of EGCG-dispersed selenium nanoparticles
Kubicova et al. Quinolinic acid: neurotoxin or oxidative stress modulator?
Liu et al. Safe and effective antioxidant: the biological mechanism and potential pathways of ergothioneine in the skin
Shabbir et al. Synthesis of iron oxide nanoparticles from Madhuca indica plant extract and assessment of their cytotoxic, antioxidant, anti-inflammatory, and anti-diabetic properties via different nanoinformatics approaches
Cheng et al. Simultaneous detection of mitochondrial hydrogen selenide and superoxide anion in HepG2 cells under hypoxic conditions
Mondal et al. Evaluation of in vitro antioxidant, anticancer and in vivo antitumour activity of Termitomyces clypeatus MTCC 5091
Prachayasittikul et al. Elucidating the structure-activity relationships of the vasorelaxation and antioxidation properties of thionicotinic acid derivatives
Samsonowicz et al. Enhanced antioxidant activity of ursolic acid by complexation with copper (II): Experimental and theoretical study
Ziemlewska et al. Assessment of cosmetic properties and safety of use of model washing gels with Reishi, Maitake and Lion’s Mane extracts
CN105193830A (zh) 3’,6-二芥子酰基蔗糖酯的抗氧化活性用途
Polyakov et al. The endless world of carotenoids—structural, chemical and biological aspects of some rare carotenoids
Kongsompong et al. Computer-aided virtual screening and in vitro validation of biomimetic tyrosinase inhibitory peptides from abalone peptidome

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20160316