CN105392372A

CN105392372A - Granules with small smooth cores

Info

Publication number: CN105392372A
Application number: CN201480034467.6A
Authority: CN
Inventors: N·T·贝克尔; D·M·斯科特; A·M·阿尔盖尔; S·科伊乌萨洛; M·S·格伯特; L·怀特; T·玛尔米; P·瓦哈-瓦海
Original assignee: Danisco USA Inc
Current assignee: Danisco USA Inc; Danisco US Inc
Priority date: 2013-06-19
Filing date: 2014-06-19
Publication date: 2016-03-09
Also published as: JP2016528881A; WO2014205161A1; BR112015031623A2; CA2915538A1; BR112015031623B1; AR096665A1; MX2015016529A; CA2915538C; EP3010351A1

Abstract

The present teachings provide an improved population of granules comprising small smooth core particles. Methods of making and using are also provided.

Description

There is the particle of small light slide core

The cross reference of related application

The U.S. Provisional Application USSN61/837 that patent application claims was submitted on June 19th, 2013, the benefit of priority of 122, this provisional application is incorporated herein by reference in full.

Technical field

The disclosure relates to the particle swarm containing activating agent and the preparation and application thereof of improvement.

Background technology

The dryed product preparation (such as washing agent, yarn fabric composition, baking goods, food and animal feed) be applied to by activating agent (such as enzyme) in industry and consumer's application becomes practice.Known enzyme is for decomposing spot, change fabric color and texture, change the viscosity of dough/pasta and food, and by the utilization rate that improves the nutriment such as soluble phosphate in food and animal feed and reduce the digestibility that ANFs such as phytic acid improves food and animal feed, thus improve animal production.

Enzyme deactivation may be there is between the storage life in dryed product preparation, described dryed product preparation is such as laundry detergent and dish washing detergent, yarn fabric processing blend, cure flour and by such as cereal (as corn or soybean), vitamin, the animal feed that mineral matter becomes to be grouped into nutriment (as Choline Chloride) etc., and this external industrial processes (steam granulation as animal feed) period may because being exposed to high temperature, steam or high humility, pressure or shear stress and chemical substance are (as acid, alkali, surfactant, bleaching agent or organic solvent).If enzyme after processing (such as, when cooling after steam treatment and granulation) reactivates, then inactivation is reversible at least partly; But inactivation is normally irreversible, make the catalytic activity of enzyme after processing (when such as, cooling after steam treatment and granulation) can not recover completely.The irreversible inactivation of enzyme and activity are reduced in the process of such as steam granulation normally less desirable.

Compared with dry feed mixture, feed pellets have industry favor character, the feeding quality such as improved, the pathogen of minimizing, manufacture during dust level lower, be easy to process and become distribute to more homogeneous.Preferred industrial prilling utilizes steam injection being called as in modified process, namely before granulation step, adds moisture and raised temperature, is then forced by granulation step and pass through mould through steam-heated feed ingredient or through modified wine with dregs.Prilling temperature can be about 70 DEG C to 95 DEG C or higher.

Due to steam used in granulation process, temperature, pressure and chemical substance, the activity of enzyme or effect significantly reduce usually in processing and subsequent storage process.In fact, usually supply the feed enzyme of industrial application to adopt the form of stable fluid product, it can be sprayed onto in feed pellets to avoid enzyme deactivation after granulation process.But, when using enzyme (such as by being sprayed onto on pellet by enzyme) after the pelletizing, be difficult to realize even batching, and very high for the equipment cost of adding enzyme after the pelletizing.Alternatively, can by liquid enzyme formulation or be dry mixed enzyme preparation and add in blender before granulation.In some cases, the enzyme than the more high-load needed for script can be added, to compensate the loss in granulation process.

At washing agent, yarn fabric, cure, food and feed industry all needs to stablize, durable enzyme granulate serves as product and component in pre-composition preparation, described component can store nearly several months or several years, or stand industrial processes operation such as mixing or steam treatment granulation process, and there is no obvious loss of enzyme activity.

For avoiding making in industrial processes enzyme, irreversibly inactivation or the method for problem that reduces enzymatic activity comprise the mode that the new source (such as, identifying the known enzyme in extreme thermophilic microorganisms) of qualification enzyme or qualification be used for stablizing known enzyme.Klibanov, 1983, (StabilizationofEnzymesagainstThermalInactivation, AdvancesinAppliedMicrobiology, volume29, page1-28) (Klibanov, nineteen eighty-three, " resist heat-inactivated enzyme stability ", " applied microbiology progress ", 29th volume, 1-28 page) disclose three kinds of basic modes for stabilized enzyme: (1) immobilization, (2) chemical modification and (3) add additive.But any one also disclosing in these methods of Klibanov (1983) all may cause stabilisation or stabilization removal, or completely without impact.Although previous compound method has achieved certain progress (see such as WO97/23606, WO9854980, WO9739116, WO2007044968, EP1224273B1, WO2009/102770, United States Patent (USP) 6 in this field, 602,841, EP1804592B1, EP0098631B1 and EP1996028), but instruction content of the present invention by adopt improve grain structure overcome in these problems some in achieve new progress again.

Usually desirable to provide the solid pharmaceutical preparation of the graininess or granular form with high concentration organized enzyme.Use and be designed to protective enzyme and avoid the dressing of inactivation, isolation coat and additive or excipient to prepare granular solid matter.In order at utmost reduce the cost of these protectiveness dressing, additive and excipient, and the impact of Product transport cost being reduced to minimum, iting is desirable to prepare graininess enzyme with the most suitable ratio of organized enzyme and non-active ingredient.But the granularity as fruit granule remains unchanged when enzyme concentration or pay(useful) load increase, so reduce for the number of particles of dispensing to the enzyme of the specified rate in final products or application.But the number of particles reduction of each feeding quantity too much can cause otherness between the separate charging amount of product very big.Such as, one spoonful of washing agent, yarn fabric process blend, cure and can cause the uneven distribution of enzyme concentration between separate charging amount or deal with the enzyme granulate of par low in dough/pasta or a animal feed, thus cause the difference of ferment treatment effect between each feeding quantity.

In order to obtain the economic benefit of the enzyme pay(useful) load of increase, the variability issues simultaneously avoiding the particle deficiency due to each feeding quantity to cause, can attempt the average grain diameter reducing enzyme granulate, with the particle making each feeding quantity all maintain sufficient amount.But, in the art for coating such as such as bed spray dressing, pan coating, cylinder dressing, be difficult to enzyme dressing and Protective isolation dressing to be applied on less core particle.Diameter due to core particle becomes less, is particularly less than about 250 microns, is less than 200 microns, is less than 175 microns, is less than 150 microns, and therefore the trend of particle aggregation increases.In addition, the coating material of given percentage by weight is applied on less core and will obtains thinner dressing, thus obtain the dressing of less protectiveness.The problem applying even dressing to small-sized core becomes particularly outstanding when shape of particle is irregular.Slightly pointed or have the core at the turning of corner angle and edge to apply dressing can to produce region that is thin or discontinuous dressing to tool on the surface.Being applied to dressing on irregular dressing also may adhesion, maybe can produce the aggregate that surperficial micro-bulge on wherein adjacent core particle or protrusion of surface contact with each other.Do not occurring to compensate this often increase cost by applying thicker dressing under the prerequisite of reuniting.

Thus need the method improved, enzyme dressing is applied on core by described method can not cause remarkable reunion, guarantees to obtain effectively and continuous print protectiveness dressing simultaneously.The enzyme stability of enhancing can provided between processing and storage life compared with small-particle realizing this high-quality dressing, higher pay(useful) load is provided simultaneously, and also maintain enough uniformities of mixture simultaneously, make not increase the otherness between each feeding quantity.

Summary of the invention

Target of the present invention guarantees or improve the stability of enzyme storing separately, in industry processes or in the mixture mixed with other compositions, improves the ability be applied to by the continuous coating with consistent minimum thickness (defect namely reduced or weak spot quantity be applied to core or the percentage by weight of coating material that contains on enzyme core becomes the dressing of certain relation) on particle simultaneously.

We surprisingly find, provide to have more smooth, more regular surfaces and narrow size distribution core and make the improvement dressing with consistent minimum thickness to be applied on less particle.In some embodiments, obtain the dressing enzyme granulate of improvement in the following way: be less than 2.5 from smoothness number, weight mean particle size is less than 300 microns and the core assembly that particle size distribution degree index (PSDI) is less than 2.0 is started with, and carries out dressing with the other dressing of the polymer comprising at least 7%w/w accounting for final particle to containing enzyme core.

Accompanying drawing explanation

Fig. 1 shows and instructs the smooth core particle of the exemplary small of content according to the present invention, and compares with other core particles little but irregular.

Fig. 2 shows some example data of instructing content according to the present invention.

Fig. 3 shows some example data of instructing content according to the present invention.

Fig. 4 is the figure of the granulation result showing Three Represents granules batch.Secret note represents wine with dregs activity, and dark-grey vitta and light gray vitta represent the rate of recovery at 90 DEG C and 95 DEG C respectively.

Fig. 5 is the figure of the granulation result showing Three Represents phytase standard TPT batch.Secret note represents wine with dregs activity, and dark-grey vitta and light gray vitta represent the rate of recovery at 90 DEG C and 95 DEG C respectively.

Detailed description of the invention

Except as otherwise noted, otherwise the present invention instructs the practice of content to adopt traditional Protocols in Molecular Biology (comprising recombinant technique), microbiological technique, cytobiology technology, Measurement for Biochemistry, granulation technique and animal feed granulating technique, and these technology are all within the scope of art technology.These technology have carried out complete explaination in the following documents, such as molecularCloning:A (" Molecular Cloning: A Laboratory refers to for LaboratoryManual, secondedition (Sambrooketal., 1989) south ", the second edition, the people such as Sambrook, 1989); OligonucleotideSynthesis (M.J. gait, ed., 1984) (" oligonucleotides synthesis ", M.J.Gait edits, 1984); Current protocolsinMolecularBiology (F.M.Ausubeletal., eds., 1994) (" modern molecular biological experiment technology ", the people such as F.M.Ausubel edit, 1994); pCR:The polymeraseChainReaction (Mullisetal., eds., 1994) (" PCR: polymerase chain is anti- should ", the people such as Mullis edit, 1994); geneTransferandExpression:A laboratoryManual (Kriegler, 1990) (" transgenosis and expression: laboratory manual ", kriegler, nineteen ninety), GranulationTechnologyforBioproducts (Kadam, 1990) (" biological product granulation technique ", Kadam, nineteen ninety), and Fairfield, D.1994.Chapter10, PelletingCostCenter.InFeedManufacturingTechnologyIV. (McEllhiney, editor), AmericanFeedIndustryAssociation, Arlington, Va., pp.110-139 (Fairfield, D., 1994, " feed manufacturing technology IV " the 10th chapter granulation cost centre, McEllhiney edits, U.S. feed TIA, Virginia Allihn pauses, 110-139 page).

Unless definition herein in addition, otherwise the implication of all scientific and technical terminologies used herein and the present invention instruct content those of ordinary skill in the field usually to understand the same.Singleton, etal., DictionaryofMicrobiologyandMolecularBiology, seconded., JohnWileyandSons, NewYork (the 1994) (people such as Singleton, " microbiology and molecular biology dictionary ", 2nd edition, John Wei Li publishing house, New York, 1994), and Hale & Markham, TheHarperCollinsDictionaryofBiology, HarperPerennial, NY (1991) (Hale and Markham, " Harper Collins biology dictionary ", HarperPerennial publishing house, New York, 1991) provide the universaling dictionary of many terms used in the present invention for technical staff.With methods described herein and material type like or any method of being equal to and material all can be used for practice or the test that the present invention instructs content.

For ease of reference, we describe the key element that the present invention instructs content under one or more title.The instruction content that it should be noted that under each title is also applicable to the instruction content under other titles.Such as, relate to the present invention to instruct each described embodiment of the use of content and aspect to be equal to relate to the present invention and instruct the method for content or the present invention to instruct embodiment or the aspect of the composition of content.Equally, relate to the present invention to instruct each described embodiment of the method for content or purposes and aspect to be equal to relate to embodiment or the aspect that the present invention instructs the composition of content.In the present patent application, unless clearly indicated in addition, otherwise the use of odd number comprises plural number.

The all patents quoted herein, patent application, publication, document and article are all incorporated to herein in full with way of reference.

Number range provided herein comprises the end value of limited range.

definition

As used herein, term " particle " refer to comprise core (being generally small light slide core particle), activating agent (being generally enzyme) and optionally at least one add the particle of dressing.

As used herein, term " core " refers to the kernel of particle, and generally includes " small light slide core particle ".The present invention instructs the core of content to make by multiple manufacturing technology, and described manufacturing technology comprises: rotary-atomizing, wet granulation, dry granulation, spraying dry, disk pelletizing, extrude, pan coating, round as a ball granulation, cylinder granulation, fluid bed agglomeration, high shear granulation, bed spray dressing, crystallization, precipitation, emulsified gel method, eddy-current disc atomization and Castingother method and prilling.These techniques are known in the art, and in U.S. Patent No. 4689297 and U.S. Patent No. 5324649 (fluid bed processing); EP656058B1 and U.S. Patent No. 454332 (expressing technique); U.S. Patent No. 6248706 (granulation, high shear); And described by having in EP804532B1 and U.S. Patent No. 6534466 (utilizing the group technology of fluid bed core and blender dressing).

Activating agent is usually coated around core.Be suitable for any material that the present invention instructs the core of content can be to meet the definition of smoothness number as herein provided, mass median diameter and particle size distribution degree.Instruct in an embodiment of content in the present invention, described core comprises sodium chloride or sodium sulfate crystal.Instruct in another embodiment of content in the present invention, described core comprises sucrose crystal seed.The particle be made up of inorganic salts and/or carbohydrate and/or little organic molecule can be used as the core that the present invention instructs content.The suitable water-soluble composition be used for incorporation in core comprises: inorganic salts are sodium chloride, ammonium sulfate, sodium sulphate, magnesium sulfate, zinc sulfate such as; Or urea; Citric acid; Carbohydrate is sucrose, lactose etc. such as.In some embodiments, core particle is crystalline inorganic salt.In certain embodiments, core particle is sodium sulfate crystal.

" small light slide core particle " refers to that display has the particle of some smoothness number, mass median diameter and particle size distribution degree index determination as provided herein as the term is employed herein, the normally internal particle of particle.

" smoothness number " refers to the ratio of the covering specific area that experience specific area calculates with the representative sample for core as the term is employed herein.

As used herein, " representative sample of core " refers to the chance sample of at least 15 core particles.

" experience specific area " refers to every gram of surface area (measuring with meters squared per gram) of the representative core product rule of thumb measured as the term is employed herein, as used BET (Brunauer-Emmett-Teller) gas adsorption method (S.Brunauer, P.H.EmmettandE.Teller, J.Am.Chem.Soc., 1938,60,309.doi:10.1021/ja01269a023) (S.Brunauer, P.H.Emmett and E.Teller, " JACS ", 1938,60th volume, the 309th page, DOI:10.1021/ja01269a023) record.The detail of BET method is described in " method " part hereafter.

" covering specific area " refers to the idealized hypothesis using all core particles to be standard ball as the term is employed herein, the specific area (unit is meters squared per gram) that the size distribution recorded and average real density based on representative core product calculate.For calculating the arthmetic statement of covering specific area in " method " part hereafter.

" size distribution " (being abbreviated as PSD) refers to the relative quantity by mass percentage of the small light slide core particle in each being present in some particle size interval as the term is employed herein.The PSD of core product passes through laser light scattering (see such as T.Allen, ParticleSizeMeasurement, Vol.1 (ChapmanandHall, 1997) (T.Allen, " granulometry ", the 1st volume, Chapman and Hall, 1997) measure.In this application, PSD is characterized by three kinds of percent mass position diameters, and percent mass position diameter can be determined by the lognormal distribution plot of PSD.

D ₅₀or MMD (mass median diameter): logarithm normal distribution mass median diameter.MMD is regarded as mass median diameter, and in representative sample, the particle of 50%w/w has the diameter lower than this value.

D ₁₀: the tenth hundredths diameter (TPD), namely in representative sample, the particle of 10%w/w has the diameter being less than this value.

D ₉₀: the 90 hundredths diameter (NPD), namely in representative sample, the particle of 90%w/w has the diameter being less than this value.

Usually, D ₁₀, D ₅₀and D ₉₀represent with micron.

The D that " particle size distribution degree index " (being abbreviated as PSDI) of core product is representative sample ₉₀/ D ₁₀ratio.

" dressing " refers to the layer of material around basic material as the term is employed herein.First coatings encapsulates core usually to form continuous print layer substantially, makes wicking surface have few non-coating area or not have non-coating area, and is generally activating agent dressing.Follow-up added coating layer can encapsulate the particle of growth, to form one or more extra play of continuous print substantially (" additional dressing ").Material (such as, activating agent detailed in this article and component) used in particle (but without the need to) can be suitable for food and/or animal feed, therefore can be food-grade or feed grade.

The present invention instruct the dressing of content also can comprise following in one or more: inorganic salts, organic acid salt, sugar, sugar alcohol, starch (native starch, pre-gelatinized starch, hydrolyzed starch or converted starch) and other polysaccharide, natural gum, other activating agent, feed grade or food grade polymer, pigment, clay, plasticizer, surfactant, fibrous material, antiplastering aid, filler, extender and become known for other compounds of dressing.Suitable polymer comprises polyvinyl alcohol (PVA), comprise the PVA of part and complete hydrolysis, polyethylene glycol, PEO, polyvinylpyrrolidine and carbohydrate polymer (such as starch, amylose, amylopectin, alpha-glucans and beta glucan, pectin, glycogen), also comprise its mixture and derivative.Comprise for increasing stacking volume for the suitable filler of dressing and to reduce costs or for regulating the inert material of the expection enzymatic activity in the particle that finally obtains.The example of this type of filler includes but not limited to aqueous solvent (such as salt, carbohydrate) and water dispersant (such as clay, talcum, silicate), cellulose and starch, and cellulose and starch derivatives.Instruct for the present invention the suitable plasticizers of the dressing of content to be low molecular weight organic compound, and for plasticized polymer, there is high degree of specificity.Example includes but not limited to that carbohydrate (such as, glucose, fructose and sucrose), sugar alcohol (such as, D-sorbite, xylitol and maltitol and other glycol), polar low molecular weight organic compounds such as urea, or other known plasticizer such as water or feed grade plasticizer.The suitable fiber materials of the dressing of content is instructed to include but not limited to for the present invention: cellulose, and cellulose derivative such as HPMC (HYDROXY PROPYL METHYLCELLULOSE), CMC (carboxy methyl cellulose), HEC (hydroxy ethyl cellulose).Instruct in an embodiment of content in the present invention, especially for feed applications, dressing comprises the corn ear material of water-soluble or water dispersible or sugar or salt crystal.In another embodiment being particularly suitable for household cleaning application, dressing comprises the sugar of water-soluble or water dispersible or salt crystal or sugared ball (nonpareil).Person of skill in the art will appreciate that, for feed and food applications, dressing (and any polymer, filler, plasticizer, fibrous material and extender) is acceptable for food and/or feed applications.For household cleaning application, do not need to apply this type of restriction.

" activating agent dressing " refers to the dressing comprising activating agent (being generally enzyme) as the term is employed herein.Usually, activating agent dressing is applied on small light slide core particle, then optionally applies additional dressing.

" additional dressing " refers to one or more layers dressing be optionally applied on small light slide core particle as the term is employed herein.Usually, additional dressing is applied to the new grain comprising small light slide core particle and activating agent dressing.The non-limitative example of additional dressing comprises waterproof dressing and moisturizing dressing.

" waterproof dressing " refers to the dressing comprising waterproof material as the term is employed herein.

" moisturizing dressing " refers to the dressing comprising moisturizing material as the term is employed herein.

The material that term " waterproof material " refers to eliminating, prevents or substantially hinder water to absorb.These materials are generally hydrophobic or amphipathic, can provide the isolation to water, can not absorb inherently and/or Bound moisture, and include but not limited to filmogen.The example of waterproof material comprises barrier polymer, protein, lipid, fat and oil, aliphatic acid and natural gum.The example of film water-proofing material is become to be natural and the barrier polymer (such as gum arabic, whey, WPC) of modification, PVA (comprising modified PVA and all-hydrolytic PVA) and synthetic polymer (such as latex, HPMC and acidolysis hydroxypropul starch, such as, PureCote ^tM, oxidized starch and modified starch).Non-film waterproof material comprises such as wax, fat, oil and lipid and lecithin.The selected waterproof material being not easy to be oxidized is for such as latex polymer and barrier polymer are as gum arabic.

Term " moisturizing material " refers to the material being absorbed liquid, aqueous such as water by one of number of mechanisms.In the first non-limiting mechanism, this material absorbs free water.In the second non-limiting mechanism, this material absorbs the Bound moisture usually existed with the crystallization water form of hydrate.Therefore, this material can be used as the material of partially or completely hydration or provides as non-hydrated material, and it is by absorption or in conjunction with liquid, aqueous, and delays or reduce this type of liquid movement to the speed of activating agent or degree.In the 3rd non-limiting mechanism, moisturizing material is by postponing the heat trnasfer that carries out to intragranular activating agent and by under activating agent being maintained the temperature lower than particle exterior surface temperature, making activating agent thermal insulation.Moisturizing material comprises carbohydrate and inorganic salts, comprises hydrated salt, as magnesium sulfate, sodium sulphate and ammonium sulfate; Maltodextrin; Carbohydrate, as sucrose; Starch, comprises cornstarch.

As used herein, term " pellet " and " granulation " refer to solid, circle, spherical and cylindrical tablet or globule, and for the formation of this type of solid shape, especially the technique of animal feed extruded by feed pellets and solid.Known food and animal feed granulation manufacturing process generally include and food or feed ingredient are at room temperature mixed together about 1 to about 5 minute; gained mixture is transferred to buffering bucket; mixture is transported to steam regulation of texture, conditioning of texture device; optionally the mixture through steam regulation of texture, conditioning of texture is transferred to expander; mixture is transferred to comminutor or extruder, finally pellet is transferred in granulate cooler.Fairfield, D.1994.Chapter10, PelletingCostCenter.InFeedManufacturingTechnologyIV. (McEllhiney, editor), AmericanFeedIndustryAssociation, Arlington, Va., pp.110-139 (Fairfield, D., 1994, " feed manufacturing technology IV " the 10th chapter granulation cost centre, McEllhiney edits, U.S. feed TIA, Virginia Allihn pauses, 110-139 page).

As used herein, term " non-granulated mixture " refers to pre-composition or precursor, base material mixture, wine with dregs and diluent.Pre-composition comprises vitamin and trace mineral usually.Base material mixture comprises food and feed composition usually, such as Dicalcium Phosphate, lime stone, salt and vitamin and mineral pre-mix, but does not comprise cereal and protein component.Diluent includes but not limited to cereal (such as wheat bran and rice bran) and clay such as phyllosilicate (magnesium silicate sepiolite, bentonite, kaolin, montmorillonite, hectorite, talcum powder, beidellite, attapulgite and humite).Clay also serves as the carrier of feed premix and fluidizing reagent or diluent.Wine with dregs generally includes complete animal feed.

As used herein, term " activity of recovery " refers to both ratio following: (i) activating agent is in activity after treatment, this process relates to one or more stressors following: pH, storage, the drying of heating, the pressure increased, the pH of rising, reduction, be exposed to one or more surfactants, be exposed to one or more solvents (comprising water/moisture) and mechanical stress, with (ii) phytase activity before treatment.The activity recovered can be expressed as percentage.

The percent activity recovered is calculated as follows:

In the background of granulation experiment, " activity before treatment " is estimated by measuring the bioactivator activity be present in undressed wine with dregs, and described estimation mode is mated in other respects with the mode of treated activating agent.

Such as, under the condition similar with the activating agent in the wine with dregs of treated (such as, granulation), the activating agent in undressed wine with dregs disposed and store the similar time, to control possible interaction or other influences beyond particular procedure itself.

As used herein, term " activating agent " can be to be provided for any material of the expectation function of given purposes to be added to particle.Activating agent can be substitute reagent, prebiotics, probio, agrochemical ingredients (such as pesticide, fertilizer or herbicide), drug ingedient, household care active component or their combination at biologically feasible material, food or feed ingredient, antimicrobial, antibiotic.In a preferred embodiment, active component is protein, enzyme, peptide, polypeptide, amino acid, carbohydrate, lipid or oil, vitamin, B B-complex, hormone or their combination.In another embodiment, active component is enzyme, bleaching agent, bleach-activating, spices or other biological active component.Inherent heat-staple activating agent is covered by the present invention and instructs in content, and can show the heat endurance with enhancing in particle.Most preferred active component for food and feed application is enzyme, peptide and polypeptide, amino acid, antimicrobial, intestinal health promoter, vitamin and their combination.Can use any enzyme, and the non-limiting list of enzyme comprises phytase, zytase, 1,4 beta-glucanase, phosphatase, protease, amylase (AMS or beta amylase or glucoamylase), cellulase, lipase, cutinase, oxidizing ferment, transferase, reductase, hemicellulase, mannonase esterase, isomerase, pectase, lactase, peroxidase, laccase, other oxidoreducing enzyme and their mixture.Particularly preferred enzyme comprises the zytase from trichoderma reesei (Trichodermareesei) and the zytase variant (the two all derives from DuPontIndustrialBiosciences) from trichoderma reesei, or the heat-staple zytase in inherence described in EP1222256B1, and from aspergillus niger (Aspergillusniger), aspergillus albicans (Aspergilluskawachii), Tabin aspergillus (Aspergillustubigensis), rod aspergillus (Aspergillusclavatus), Bacillus circulans (Bacilluscirculans), bacillus pumilus (Bacilluspumilus), bacillus subtilis (Bacillussubtilis), wheel branch sickle-like bacteria (Fusariumverticilloides), Fusarium oxysporum (Fusariumoxysporum), Rumen Fungi (Neocallimastixpatriciarum), Penicillium spp (Penicilliumspecies), muta lead mycillin (Streptomyceslividans), the purple streptomycete of heat (Streptomycesthermoviolaceus), thermomonospora fusca (Thermomonosporafusca), Trichoderma harzianum (Trichodermaharzianum), trichoderma reesei, other zytases of Trichoderma viride (Trichodermaviride).Particularly preferred enzyme in addition comprises phytase, such as, from the phytase Finase of Aspergillus sp (Aspergillussp.) derive from ABEnzymes, Darmstadt, Germany; From the phytase Phyzyme of Escherichia coli (E.Coli) ^tMxP and the phytase AxtraPhy from cloth mound Salmonella (Buttiauxella), the two all derives from DaniscoAnimalNutrition, DuPont; Other phytases from such as following organism: trichoderma (Trichoderma), Penicillium (Penicillium), Fusarium (Fusarium), Buttiauxella, Citrobacter (Citrobacter), Enterobacter (Enterobacter), Penicillium, Humicola (Humicola), Hafnia (Hafnia), bacillus (Bacillus) and every spore volt leather belong to (Peniophora); And U.S. Patent application 61/595,923 and 61/595, those phytases described in 941, these two sections of patent applications are all filed on February 12nd, 2012.The example of cellulase is bGL, this is that one derives from DaniscoAnimalNutrition, the cellulase (beta glucan enzyme) of DuPont; And other cellulases of species from such as aspergillus, trichoderma, Penicillium, Humicola, bacillus, Cellulomonas (Cellulomonas), Penicillium, Thermomonospora (Thermomonospore), fusobacterium (Clostridium) and Hypocrea (Hypocrea).Also can use the cellulase described in US20060193897A1 and endoglucanase.Amylase can such as from the species of such as aspergillus, trichoderma, Penicillium, bacillus (such as bacillus subtilis, bacillus stearothermophilus (B.stearothermophilus), bacillus lentus (B.lentus), bacillus licheniformis (B.licheniformis), bacillus coagulans (B.coagulans) and bacillus amyloliquefaciens (B.amyloliquefaciens)).Suitable fungal amylase derives from aspergillus, such as aspergillus oryzae (A.oryzae) and aspergillus niger (A.niger).Protease can from bacillus amyloliquefaciens, bacillus lentus, bacillus subtilis, bacillus licheniformis, and aspergillus and trichoderma species.Phytase, zytase, phosphatase, protease, amylase, esterase, oxidoreducing enzyme, lipase, transferase, cellulase and 1,4 beta-glucanase are the enzymes being usually used in adding in animal feed.It is similar for being suitable for adding the household care enzyme applied in tablet used to, especially protease, amylase, lipase, pectate lyase, mannonase hemicellulase, oxidoreducing enzyme, peroxidase, transferase and cellulase.The present invention instruct content particularly preferred in, enzyme is selected from phytase, zytase, 1,4 beta-glucanase, amylase, protease, lipase, esterase and their mixture.In one embodiment of the invention, in particle, two kinds of enzymes are provided, i.e. zytase and 1,4 beta-glucanase.In another embodiment of the invention, the two kinds of enzymes provided in particle are protease and amylase.Described enzyme can be mixed, or be applied to particle separately.In another embodiment, in particle, three kinds of enzymes are provided, i.e. 1,4 beta-glucanase, zytase and phytase.Enzyme listed above is only and illustrates, and does not mean to be exclusiveness.Any enzyme all can be used for the present invention and instructs in the particle of content, comprises bacterium, fungi, yeast, plant, the wild-type enzyme of insect and animal origin, recombinase and variant enzyme, and acidicenzym, neutral enzymatic or alkaline enzyme.Those skilled in the art will appreciate that the amount of enzyme used will depend on the type of selected enzyme and character and desired use thereof at least in part.

method

bET specific surface area is measured.

In order to measure the specific area of core particle, use BET method to measure Krypton by the quick porosity of MicromeriticsASAP2420 type and Surface Area Analyzer (AcceleratedPorosimetryandSurfaceAreaSystem) to adsorb/desorption assesses the distribution of pores of core.BJH method ( elliottP. barrett, leslieG.Joyner, paulP.Halendaj.Am.Chem.Soc., 1951,73 (1), pp373 – 380, DOI:10.1021/ja01145a126) (ElliottP.Barrett, LeslieG.Joyner and PaulP.Halenda, " American Chemical Society's meeting will ", nineteen fifty-one, the 73rd volume the 1st phase, 373rd – 380 pages, DOI:10.1021/ja01145a126) for being determined at pore volume distribution in scope.Be that sample vacuum outgas at 150 DEG C of temperature of about 4-6 gram spends the night, to remove water and/or the volatile contaminant of absorption by quality.Sample weighed again and is transferred to porosimeter, under being placed in vacuum, being cooled to 77K, now by pressure measxurement record gas (Kr) adsorption isotherm.The automatic computational chart area coverage of the ASAP2420V2.07J software kit provided by Micromeritics.

covering specific area calculates.

The specific area of the idealized sample of complete smooth, spherical particle that the representative of covering specific area is equivalent, the PSD of this spheroidal particle mates with the PSD of actual core product.By laser diffraction (with reference to ISO13320-1:1999), use MalvernMastersizer2000 and measure the size distribution (PSD) of core using hexane as the BACKGROUNDFluid of recirculation circuit.The core sample size of adding enough quality is covered with the laser reaching about 10%.Mie theory is used automatically to calculate PSD by diffraction pattern.With regard to sodium sulphate, the complex refractivity index of particle is taken as 1.468+0.01i, and the refractive index of fluid is taken as 1.375.The PSD generated is differential mass mark, to show in each of the hopper increased with logarithm 81 spacing size at 0.01 micron of percentage to the particle in 10,000 micrometer range.If necessary, it is false as such as dust fines or aggregate to get rid of that reply PSD carries out corrections renormalization, is especially narrower than 60 microns or be greater than any particle of 500 microns.

The upper dimension bound of the hopper of often kind of size and the geometric mean diameter of lower size limit are calculated by the square root getting the product of upper dimension bound and lower size limit.Drawn the covering specific area (in meters squared per gram) of given hopper by 6/ (d* ρ), wherein d is particle diameter (in cm), ρ is that the real density (getting rid of the density behind space) of fine-particle solid is (with g/cm ³meter).With regard to sodium sulphate, ρ=2.664g/cm ³.Because PSD is quality weight distribution, therefore by the differential mass mark of often kind of size hopper being multiplied by the specific area of corresponding size hopper, then the value of all size hoppers is sued for peace, thus it is long-pending to calculate total cladding surface.

exemplary

In some embodiments, the present invention instructs content to provide a kind of particulate composition, and it has equivalent stability surprisingly compared to the particle containing non-small light slide core, thus can save material cost.Such as, the present invention instructs the particulate composition of content can have surprising equivalent storage stability.Required storage stability can be present in any one of multiple environment, comprises dish washing detergent, laundry detergent compositions, animal feed, yarn fabric and human food.The present invention instruct the particle of content can show under these different conditions with same store do not instruct the Particle Phase of the small light slide core particle of content than the stability improved containing the present invention.In some embodiments, the present invention instruct content it is believed that to provide and Particle Phase containing larger and/or Non-smooth surface core than the stability improved.

In some embodiments, the present invention instructs content to provide with the Particle Phase containing non-small light slide core than the particulate composition with the anti-dust generative capacity be equal to, as recorded by Heubach dust.

In some embodiments, the present invention instructs content it is believed that to provide with the Particle Phase containing non-small light slide core than the particulate composition of anti-dust generative capacity with improvement.

In some embodiments, the present invention instructs content to provide with the Particle Phase containing non-small light slide core than the particulate composition with the steam granulation tolerance be equal to.Such as, the particle of content can be instructed to measure the degree of " activity of recovery " as hereinbefore defined for the present invention, and from containing instructing the different core of the small light slide core particle of content with the present invention but particle through same treatment compares.In some embodiments, the present invention instructs content it is believed that to provide with the Particle Phase comprising small light slide core than the particulate composition of steam granulation tolerance with improvement.

The present invention is adopted to instruct described by the other application of the particle of content and method have in non-limiting part hereafter.

for curing the composition and method prepared with food

The present invention instructs content also to relate to " food compositions ", include but not limited to comprise the food product of particulate composition of the present invention, animal feed and/or food/feed addictive, and for the preparation of the method for this type of food compositions, or its purposes, described method comprises and being mixed with one or more food compositions by particulate composition of the present invention.

Particulate composition of the present invention can be used for preparing food compositions, and wherein said food compositions cures after interpolation particulate composition of the present invention.As used herein, term " cures composition " and refers to any composition and/or additive prepared in the process providing baked goods product, includes but not limited to Strong flour, dough/pasta, cures additive and/or baked product.Food compositions or additive can be liquid or solid.

As used herein, term " flour " refers to through the grain of milling or grind.Term " flour " also can refer to through grinding or the sago smashed to pieces or stem tuber product.In some embodiments, flour also can comprise the component except the cereal through milling or smash to pieces or plant material.The example of other component is leavening agent, but this example not intended to be limiting.Grain comprises wheat, oat, rye and barley.Stem tuber product comprises tapioca, tapioca starch and custard powder.Term " flour " also comprises thin corn flour, corn flour, glutinous rice powder, wholemeal, self-raising flour, tapioca, tapioca starch, ground rice, fortified flour and custard powder.

For for baking and banking up with earth and the commercialization of food production and family expenses flour, the enzymatic activity in flour is maintained proper level very important.Activity level is too high may cause product to be clamminess and/or soft thick, thus cannot list marketing.The flour of deficient enzyme activity may not comprise for the enough carbohydrates of normal yeast function, and it causes bread or bakes and banks up with earth product overdrying or cracked.Therefore, can the particulate composition of the present invention be combined with one or more AMSs be added in flour, strengthen the activity level of endogenous enzymes in flour.

Amylase in particulate composition of the present invention can add separately, also can add in conjunction with other amylase, for avoiding or postponing baked product aging (i.e. interior tissue sclerosis).Anti-aging diastatic amount usually in the scope of every kilogram of flour 0.01 to 10mg zymoprotein, as 0.5mg/kg (dry).Spendable anti-aging amylase additionally comprises endo-amylase, as the bacterium endo-amylase that bacillus produces.This extra amylase can be the another kind of maltogenic alpha-amylase (EC3.2.1.133) that such as bacillus produces. be exemplary maltogenic alpha-amylase, it is produced by the NCIB11837 bacterial strain of bacillus stearothermophilus, described by having in such as Christophersenetal. (1997) Starch50:39-45.Other examples of anti-aging endo-amylase comprise the bacterialα-amylase deriving from bacillus such as bacillus licheniformis or bacillus amyloliquefaciens.Anti-aging amylase can be exo-amylase, such as beta amylase, and this exo-amylase such as comes from plant, as soybean, or comes from microorganism, as bacillus.

Comprise curing composition and also can comprising phosphatidase of particulate composition of the present invention, or there is the enzyme of activity of phospholipase.There is the available lipase unit of activity (LU) metering of the enzyme of activity of phospholipase.Phosphatidase can have A ₁or A ₂activity, so can remove aliphatic acid from phosphatide, forms lysophosphatide.Phosphatidase may be with or without lipase active (that is, to the activity of triglycerides substrate).Under normal circumstances, phosphatidase is made to have within the scope of 30 to 90 DEG C, as the optimum temperature within the scope of 30 to 70 DEG C.The phosphatidase added may come from animal, such as, come from pancreas (pancreas as ox or pig), snake venom or bee venom.Or phosphatidase may come from microorganism, such as, come from filamentous fungi, yeast or bacterium.

The phosphatide enzyme amount of adding can improve bread in the pliability of curing the rear initial stage (especially in initial 24 hours).The amount of phosphatidase usually in the scope of every kilogram of flour 0.01 to 10mg zymoprotein, as 0.1 to 5mg/kg.That is, activity of phospholipase is usually in the scope of every kilogram of flour 20 to 1000LU, one of them lipase unit (LU) is defined as taking gum arabic as emulsifying agent, tributyrin is substrate, under 30 DEG C of conditions with pH7.0, enzyme amount of disengaging needed for 1 μm of ol butyric acid per minute.

The composition of dough/pasta generally includes the meal of sizing or wheat flour and/or other types, flour or starch, such as corn flour, cornstarch, rye meal, rye flour, oat flour, oat meal, soy meal, sorghum meal, Chinese sorghum flour, potato meal, potato flour or farina.Dough/pasta can be fresh, freezing or part is cured.Dough/pasta may be fermented-dough/pasta or the dough/pasta preparing fermentation.Dough/pasta can ferment in many ways, such as, by adding chemical leaven as sodium acid carbonate or by adding fermentate (i.e. unrisen dough) fermentation.Dough/pasta also ferments by adding suitable yeast culture such as the culture of saccharomyces cerevisiae (Saccharomycescerevisiae) (Saccharomyces cerevisiae), wherein saccharomyces cerevisiae bacterial strain as commercially available in it.

Dough/pasta also can contain the dough ingredients of other routines, and such as protein, as milk powder, glutelin and soybean protein; Eggs (as shell egg, yolk or egg white); Oxidant, as ascorbic acid, potassium bromate, Potassiumiodate, Celogen Az (ADA) or ammonium persulfate; Amino acid, as Cys; Sugar; Or salt, as sodium chloride, calcium acetate, sodium sulphate or calcium sulfate.Dough/pasta also can comprise fat (i.e. triglycerides), as Grainy fat tissue or shortening.Dough/pasta also can comprise emulsifying agent, as the acetic acid esters of the lactate of the polyglycerol ester of the sugar ester of the diacetyl tartrate of monoglyceride or diglyceride, monoglyceride or diglyceride, aliphatic acid, aliphatic acid, monoglyceride, monoglyceride, Myrj 45 or lysolecithin.Such as, dough/pasta can make when not adding emulsifying agent.

Dough product can be any treated dough product, comprises the dough/pasta decocted, explode, bake, bake and bank up with earth, steam and boiled, as steamed bun and rice cake.In one embodiment, food product is baked product.Typically cure (roasted) product and comprise bread, as at the bottom of loaf, roll, bun, bagel, pizza cake etc.; Face cake, as pretzel, tortilla, cake, cookie, soft cake, crispbread etc.

Optionally, extra enzyme can be used together with anti-aging amylase and phosphatidase.Extra enzyme can be the second amylase, as amyloglucosidase, beta amylase, cyclodextrin glucanotrasferase enzyme; Also can be peptase (especially exopeptidase), TGase, lipase, cellulase, zytase, protease, protein disulfide isomerase (disclosed in WO95/00636 protein disulfide isomerase), such as, glycosyl transferase, branching enzyme (1,4-alpha-glucans branching enzyme), 4-alpha-Glucanotransferase (dextrin glycosyl transferase) or oxidoreducing enzyme, as peroxidase, laccase, glucose oxidase, pyranose oxidase, LOX, L-amino acid oxidase or carbohydrate oxidase.Extra enzyme can come from any biology, comprises mammal and plant, especially can come from microorganism (bacterium, yeast or fungi), and the available ordinary skill in the art obtains.

Zytase comes from microorganism usually, such as, comes from bacterium or fungi, as the bacterial strain of aspergillus.Zytase such as comprises and Novozym these two kinds of zytases are the xylanase preparations produced by trichoderma reesei, commercially available acquisition.Amyloglucosidase can be aspergillus niger produce amyloglucosidase (as ).Other available amylase products comprise a1000 or A5000 (GrindstedProducts, Denmark), and h or p (DSM).Glucose oxidase can be mycetogenetic glucose oxidase, especially aspergillus niger produce glucose oxidase (as ).Exemplary protease comprises

Said process can be used for any kind baked product prepared by dough/pasta, and described baked product can be soft or crisp, is white, light color or dark type.Example is bread, especially white whole wheat or the rye bread of loaf or roll form is generally, such as but not limited to French bread stick, pocket bread, tortilla, cake, pancake, soft cake, cookie, piecrust, rusk, steamed bun, pizza etc.

Particulate composition of the present invention can be used in premix, and this premix, except containing except flour, also comprises anti-aging amylase, phosphatidase and/or phosphatide.This premix may also contain other for improving the additive of the character of dough/pasta and/or bread, such as any one additive (comprising enzyme) mentioned above.Particulate composition of the present invention can be the component of the enzyme preparation comprising anti-aging amylase and phosphatidase, as curing additive.

Mode by packaged mix realize load delivery vector storage, process and mix.This packaged mix can contain particulate composition of the present invention.But this packaged mix also can answer the requirement of manufacturer or teacher of the curing to contain extra composition.Teacher of the curing, after particulate composition of the present invention is mixed dough/pasta, completes subsequent operation according to the normal Making programme of baked product.

We contemplate a kind of food compositions comprising particulate composition of the present invention, and this food compositions is the composition of grease, meat, lard.Opinion therefrom, term " [grease/meat/lard] composition " refers to, based on grease, meat or lard, be made up of grease, meat or lard respectively, and/or any one composition containing grease, meat or lard.Contemplate a kind of method preparing grease or meat or lard composition and/or the additive comprising particulate composition of the present invention, comprise and particulate composition of the present invention and grease/meat/lard composition and/or additive component are mixed.

This food compositions can be comprise the animal feed composition of particulate composition of the present invention, animal feed additive and/or pet food.Also contemplate a kind of method preparing this animal feed composition, animal feed additive composition and/or pet food, comprise and particulate composition of the present invention and one or more animal feed ingredients and/or animal feed additive composition and/or pet food ingredients are mixed.Particulate composition of the present invention can be used in the process preparing animal feed composition and/or animal feed additive composition and/or pet food.

Term " animal " comprises all non-ruminant animals and ruminant.In a particular embodiment, animal is non-ruminant animal, such as horse and nonruminant.The example of nonruminant includes but not limited to pig (pig) and pig (swine), as sucking pig, grower pigs, sow; Poultry, as turkey, duck, chicken, broiler chicks, laying hen; Fish, as salmon, trout, Tilapia mossambica, catfish and carp; And Crustaceans, as dried shrimp and prawn.In further embodiment, animal is ruminant, includes but not limited to ox, calf, goat, sheep, giraffe, bison, elk, elk, yak, buffalo, deer, camel, alpaca, yamma, antelope, pronghorn Antilocapra americana and blue ox.

In the context of the present invention, should be appreciated that term " pet food " is the food that confession under directions domestic animal eats, these domestic animals are such as but not limited to dog, cat, gerbil jird, hamster, chinchilla, brown rat, cavy; Birds pet, as canary, parakeet and parrot; Reptile pet, as tortoise, lizard, snake; And aquatic pet, as tropical fish and frog.

Term " animal feed composition ", " feed " and " fodder material " are used interchangeably, and can comprise and be selected from one or more following feedings: a) cereal, such as minimand (as wheat, barley, rye, oat and their combination) and/or large grain cereal, as maize or Chinese sorghum; B) cereal by-products, such as corn gluten meal, distiller's dried grain and DDGS (DDGS) (especially corn distiller's dried grain and DDGS (cDDGS) thereof) thereof, wheat bran, prostitute, wheat time powder, rice bran, rice husk, oat shell, palm kernel and citrus pulp; C) protein of such as following material is derived from: soya bean, sunflower, peanut, lupin, pea, broad bean, cotton, canola oil dish, fish meal, dried plasma albumen powder, meat meal tankage, Potato protein concentrate, whey, copra, sesame; D) to originate the grease and fat that obtain from plant and animal; E) minerals and vitamins.

make the composition and use thereof of yarn fabric destarch

Also contemplate the composition and method that use particulate composition process fabric of the present invention (e.g., to make yarn fabric destarch).The method of process fabric is (see such as U.S. Patent No. 6,077,316) well-known in the art.For example, the solution of fabric with particulate composition of the present invention can be contacted, improve feel and the outward appearance of fabric in this approach.Can under stress with this solution-treated fabric.

Particulate composition of the present invention can apply during knitted textile or after knitting out yarn fabric, during desizing stage or during one or more other cloth treatment step.In the process of knitted textile, the yarn of fabric will bear sizable mechanical stress.Before with weaving loom braiding, usually starching starch or starch derivatives are applied to warp yam surface, improve the tensile strength of warp thread, avoid fracture.After-applied particulate composition of the present invention or can be woven during weaving, to remove these starching starch or starch derivatives.If complete after-applied particulate composition of the present invention in braiding to eliminate size coat, fabric can be processed further with that, guarantee that it possesses uniform quality and good water-wash resistance.

Particulate composition of the present invention can be used alone, also can use (in this case as detergent additives) together with other destarch chemical reagent and/or destarch enzyme, such as, make cloth (comprising containing cotton cloth) destarch with the form of waterborne compositions.Particulate composition of the present invention is also used in the composition and method that the denim fabric of indigo dyeing and clothes produce granite-wash outward appearance.Just make clothes, may first cutting cloth, made as clothes or clothes, then arranged these clothes or clothes.Specifically, multiple different tissues and organs method has been developed to make jeans.Arrange jeans usually to start with enzyme process Desizing Step, during this step, under amylolytic enzyme effect, the cloth of clothes becomes soft, and cotton in cloth after this step, the process of more acceptant follow-up tissues and organs step.The method that particulate composition of the present invention can be used for arranging jeans (as " biological technique for grinding ", enzyme desizing method, makes the limpen method of cloth and/or finishing technique.

cleasing compositions

An aspect of composition of the present invention and method is Cleasing compositions, and it comprises particulate composition of the present invention as component.Protease polypeptide or other relevant enzymes can be used as the component of composition of detergent, these composition of detergent are used for washing one's hands, do washing, wash dish and other hard surfaces clean.

Preferably particulate composition of the present invention is mixed washing agent with Acarbose concentrations conventional in washing agent or with the concentration of approximate this concentration.Such as, the addition of protease polypeptide may correspond to often liter of cleaning solution/Dishwashing liquids interpolation 0.00001 to 1mg (zymoprotein by pure) amylase.There is provided herein exemplary formula, illustrate as follows:

Protease polypeptide can be only enzyme component in composition of detergent; Or, outside composition of detergent removing protein enzyme polypeptide, also comprise other enzymes (comprising other amylolytic enzymes).Therefore, the protease polypeptide comprised in composition of detergent can be the form of particulate composition of the present invention.If employ waxy coating materi, the non-limitative example of so this material to be mean molecule quantity be 1,000 to 20,000 PEO product (polyethylene glycol, PEG); There is the ethoxylated nonylphenol of 16 to 50 ethylene oxide units; The fatty alcohol of ethoxylation, wherein alcohol is containing 12 to 20 carbon atoms, and has 15 to 80 ethylene oxide units; Fatty alcohol; Aliphatic acid; With the monoglyceride of aliphatic acid, diglyceride and triglycerides.The example being suitable for the film-forming coating materials applying fluidization is listed in such as GB1483591.

Composition of detergent can comprise one or more surfactants, and often kind of surfactant can be anionic, nonionic, cationic or amphoteric ion type.Washing agent is generally containing the anionic surfactant of 0% to about 50%, such as linear alkylbenzene sulfonate (LAS) (LAS), alpha-alkene sulfonate (AOS), alkyl sulfate (aliphatic alcohol sulfate) (AS), alcohol ethyoxysulfates (AEOS or AES), secondary alkyl sulfonate (SAS), alpha-sulfo fatty acid methyl ester, alkyl or alkenyl butanedioic acid, or soap.Composition of detergent also can containing the nonionic surface active agent of 0% to about 40%, such as alcohol ethoxylate (AEO or AE), carboxylated alcohol ethoxylates, nonyl phenol ethoxylate, APG, alkyl dimethyl amine oxide, ethoxylated fatty acid MEA, fatty acid monoethanolamide or polyhydroxy alkyl fatty acid acid amides (such as described in WO92/06154).

Composition of detergent additionally can comprise one or more other enzymes, the another kind of protease be such as combined, another kind of amylolytic enzyme, cutinase, lipase, cellulase, pectate lyase, Perhydrolase, mannonase zytase, peroxidase and/or laccase.

Composition of detergent can comprise detergent builders or the complexing agent of about 1% to about 65%, such as zeolite, diphosphate, triphosphate, phosphonate, citrate, NTA (NTA), ethylenediamine tetra-acetic acid (EDTA), diethylene-triamine pentaacetic acid (DTMPA), alkyl or alkenyl butanedioic acid, soluble silicate or phyllosilicate (e.g., the SKS-6 of Hoechst product).Composition of detergent also without the need to composite, that is, can be substantially free of detergent builders.Can use enzyme in multiple combination thing, especially amylase, these enzymes are with or without Starch Binding Domains; These compositions comprise the composition for laundry, washing dish, clean surface, and with starch or living beings for raw material is used for producing the composition of ethanol.

Washing agent can comprise one or more polymer.Example comprises carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), polyvinyl alcohol (PVA), polycarboxylate (as polyacrylate), maleic acid/acrylic copolymer and lauryl methacrylate/acrylic copolymer.

Composition of detergent can comprise bleach system, and this bleach system can contain H ₂o ₂source is as perborate or percarbonate, and it can combine onset with bleach-activating such as tetraacetyl ethylene diamine (TAED) or the nonanoly acyloxy benzene sulfonate (NOBS) forming peracid.Or bleach system can contain peroxy acid (peroxy acid as acid amides, imidodicarbonic diamide or sulfone type).Bleach system also can be enzyme bleach system, such as Perhydrolase, as that Perhydrolase described in international PCT patent application WO2005/056783.

The enzyme in composition of detergent stablized by the stabilizing agent of available routine, and these stabilizing agents are such as polyalcohols, as propane diols or glycerine; Sugar or sugar alcohol; Lactic acid; Boric acid or boronic acid derivatives, as aromatic boric acid ester; Can by the method compositions formulated described in such as patent WO92/19709 and WO92/19708.

Washing agent also can comprise other conventional detergent ingredients, such as fabric conditioner, comprises clay, foam booster, foam inhibitor, corrosion-resistant agent, soil suspender, anti-dirt precipitating reagent, dyestuff, bactericide, tarnish inhibitor, fluorescent whitening agent or spices again.

PH (recording in the aqueous solution of working concentration) is generally neutral or alkaline, and such as pH is about 7.0 to about 11.0.

The concrete form of the composition of detergent that instruction content of the present invention comprises will be described in more detail below.

heavy duty detergent drying/solid (HDD) laundry detergent composition

Exemplary HDD laundry detergent composition comprises detersive surfactant, comprise anionic detersive surfactant (as straight chain, side chain or random chain, the mixture of substituted or unsubstituted alkyl sulfate, alkylsulfonate, alkyl alkoxylated suifate, alkylphosphonic, alkyl phosphonate, alkyl carboxylate and/or these materials), nonionic detersive surfactant (as straight chain, side chain or random chain, substituted or unsubstituted C ₈to C ₁₈alkyl ethoxylate and/or C ₆to C ₁₂alkyl phenolic alkoxy thing), cationic detersive surfactant is (as alkyl pyridine salt compound, alkyl quaternary ammonium compound, alkyl quaternary compound, alkyl ternary sulphonium compounds, and the mixture of these materials), the mixture of amphoteric ion type and/or amphiphilic detersive surfactant (as alkanolamine sulfobetaines), amphoteric surfactant, Semi-polar nonionic type surfactant and these surfactants, auxiliary agent, comprise not phosphatic auxiliary agent (such as zeolite builder, its example comprises Wessalith CS, X zeolite, zeolite P and zeolite MAP, content 0 % by weight in the scope being less than 10 % by weight), phosphate builder (such as sodium phosphate trimer, content 0 % by weight in the scope being less than 10 % by weight), citric acid, citrate, NTA, silicate (as sodium metasilicate, potassium silicate or sodium metasilicate, content 0 % by weight in the scope being less than 10 % by weight, or phyllosilicate (SKS-6)), carbonate (as sodium carbonate and/or sodium acid carbonate, content 0 % by weight in the scope being less than 80 % by weight), and bleaching agent, comprise photobleaching agent (as sulfonation phthalocyanine phthalocyanine zinc, aluminum phthalocyanine, Xanthene dyes, and the mixture of these materials), hydrophobicity or hydrophily bleach-activating are (as dodecane acyl group hydroxy benzene sulfonate, capryl hydroxy benzene sulfonate, capryl O-hydroxybenzoic acid or its salt, 3,5,5-trimethyl acetyl base hydroxy benzene sulfonate, tetraacetyl ethylene diamine (TAED), nonanoyloxybenzene sulfonate (NOBS), nitrile quaternaries, and the mixture of these materials), (as inorganic perhydrate salts, its example comprises perborate to hydrogen peroxide source, percarbonate, persulfate, one hydration sodium salt of perphosphate or persilicate or four hydration sodium salts), preformed hydrophily and/or hydrophobicity peracid are (as percarboxylic acids and salt thereof, percarbonic acid and salt thereof, cross imidic acid and salt thereof, permonosulphuric acid and salt thereof, and the mixture of these materials) and/or bleaching catalyst (as imines bleach boosters (its example comprises iminium cations and polyion), imines amphion, modified amine, modified oxidized amine, N-sulfimide, N-imines, N-acid imide, thiadiazoles dioxide, perfluor imines, the mixture of cyclohexanol ketone and these materials, and metallic bleaching catalyst (as copper, iron, titanium, ruthenium, tungsten, molybdenum or manganese cation, its together with auxiliary metal cation (as zinc or aluminium) and chelating agent as ethylenediamine tetra-acetic acid, ethylenediamine tetramethylene phosphonic acid and their water soluble salt) onset.

This composition preferably comprises enzyme; as protease, amylase, lipase, cellulase, choline oxidase, peroxidase/oxidizing ferment, pectate lyase, mannonase cutinase, laccase, phosphatidase, lysophospholipase, acyltransferase, Perhydrolase, arylesterase, and any mixture of these enzymes.

This composition optionally comprises extra detergent ingredients, comprises perfume microcapsule, accord by starch encapsulated, toner, additional polymer (comprising the polymer and cationic polymer that maintain fabric integrity), the composition pinning dyestuff, fabric softener, brightening agent (such as C.I. fluorescent whitening agent), flocculant, chelating agent, alkoxylated polyamines, fabric deposition auxiliary agent and/or cyclodextrin.

inventory dishwashing washs (ADW) composition of detergent

Exemplary ADW composition of detergent comprises nonionic surface active agent, its content, in 0 % by weight to 10 % by weight scope, comprises ethoxylated nonionic surfactant, alcohol alkoxylates surfactant, epoxy-capped poly-(alkoxylate) alcohol or amine oxide surfactant, auxiliary agent, its content is in 5 % by weight to 60 % by weight scopes, comprise phosphate builder (as, monophosphate, diphosphate, tripolyphosphate, other oligomeric polyphosphate, sodium phosphate trimer (STPP)) and not phosphatic auxiliary agent (as amino acid pattern compound, comprise methylglycine diacetic acid (MGDA) and salt thereof and derivative, glutamic acid-N, N-diacetic acid (GLDA) and salt thereof and derivative, iminodisuccinic acid (IDS) and salt thereof and derivative, Carboxymethylinulin and salt thereof and derivative, NTA (NTA), diethylene-triamine pentaacetic acid (DTPA), B-alanine diacetic acid (B-ADA) and salt thereof), the monomer of the homopolymers of polycarboxylic acids and copolymer and the salt partially or completely neutralized, polycarboxylic acids and hydroxycarboxylic acid and salt thereof, its content is in the scope of 0.5 % by weight to 50 % by weight, the polymer of sulfonation/Carboxylation, its content in the scope of about 0.1 % by weight to about 50 % by weight, for providing dimensional stability, drying aid, its content in the scope of about 0.1 % by weight to about 10 % by weight (as polyester, especially anionic polyester, optionally use together with the other monomer with 3 to 6 degrees of functionality, these degrees of functionality are generally acid, alcohol or ester degree of functionality, be conducive to polycondensation reaction occurs, form the precursor compound of polycarbonate-polysiloxane, polyurethane-polysiloxane and/or polyureas-polysiloxane compound or these compounds, especially active cyclic carbonate ester type and urea type precursor compound), silicate, its content (comprises sodium metasilicate or potassium silicate, such as sodium disilicate, sodium metasilicate, crystalline layered silicate) in the scope of about 1 % by weight to about 20 % by weight, inorganic bleaching agents (such as perhydrate salt, as perborate, percarbonate, perphosphate, persulfate, persilicate) and organic bleaches (such as organic peroxide acid, comprise diacyl peroxide and four acyl peroxides, as diperoxy base dodecanedioic acid, diperoxy base tetradecane binary acid, diperoxy base hexadecane binary acid), bleach-activating (i.e. organic peracid precursor, its content is in the scope of about 0.1 % by weight to about 10 % by weight), bleaching catalyst (as 7-triazacyclononane manganese and related complexes, bipyridyl amine closes Co, Cu, Mn and Fe and related complexes, and pentamine acetate closes cobalt (III) and related complexes), metal care agent, its content is (as BTA, slaine and complex compound thereof, and/or silicate) in the scope of about 0.1 % by weight to 5 % by weight, enzyme, its content (as protease, amylase, lipase, cellulase, choline oxidase, peroxidase/oxidizing ferment, pectate lyase, mannonase cutinase, laccase, phosphatidase, lysophospholipase, acyltransferase, hydrolase, arylesterase, and the mixture of these enzymes) in the scope of every gram of automatic dishwashing detergent composition about 0.01 to 5.0mg organized enzyme, and enzyme stabilizers component (as oligosaccharides, polysaccharide, inorganic divalent metal salt).

other composition of detergent

In multiple numbering paragraph, hereafter describe the other exemplary detergent compositions of the various relevant enzyme can added wherein in particulate composition of the present invention.

1) be configured to a composition of detergent for particulate material, its bulk density is at least 600g/L, containing the linear alkylbenzene sulfonate (LAS) (by acid) of about 7% to about 12%; The alcohol ethyoxysulfates of about 1% to about 4% is (as C _12-18alcohol, 1 to 2 oxirane (EO) unit) or alkyl sulfate (as C _16-18alkyl sulfate); The alcohol ethoxylate of about 5% to about 9% is (as C _14-15alcohol, 7 EO unit); The sodium carbonate of about 14% to about 20% is (as Na ₂cO ₃); The soluble silicate of about 2% to about 6% is (as Na ₂o2SiO ₂); The zeolite of about 15% to about 22% is (as NaA1SiO ₄); The sodium sulphate of 0% to about 6% is (as Na ₂sO ₄); Natrium citricum/the citric acid of about 0% to about 15% is (as C ₆h ₅na ₃o ₇/ C ₆h ₈o ₇); The sodium perborate of about 11% to about 18% is (as NaBO ₃h ₂o); The TAED of about 2% to about 6%; The carboxymethyl cellulose (CMC) of 0% to about 2%; The polymer (as maleic acid/acrylic copolymer, PVP, PEG) of 0% to 3%; The zymoprotein (by pure enzyme) of 0.0001% to 0.1%; With 0% to 5% micro constitutent (as foam inhibitor, spices, fluorescent whitening agent, photobleaching agent).

2) be configured to a composition of detergent for particulate material, its bulk density is at least 600g/L, containing the linear alkylbenzene sulfonate (LAS) (by acid) of about 6% to about 11%; The alcohol ethyoxysulfates of about 1% to about 3% is (as C _12-18alcohol, 1 to 2 EO unit) or alkyl sulfate (as C _16-18alkyl sulfate); The alcohol ethoxylate of about 5% to about 9% is (as C _14-15alcohol, 7 EO unit); The sodium carbonate of about 15% to about 21% is (as Na ₂cO ₃); The soluble silicate of about 1% to about 4% is (as Na ₂o2SiO ₂); The zeolite of about 24% to about 34% is (as NaA1SiO ₄); The sodium sulphate of about 4% to about 10% is (as Na ₂sO ₄); Natrium citricum/the citric acid of 0% to about 15% is (as C ₆h ₅na ₃o ₇/ C ₆h ₈o ₇); The carboxymethyl cellulose (CMC) of 0% to about 2%; The polymer (as maleic acid/acrylic copolymer, PVP, PEG) of 1% to 6%; The enzyme (by pure enzyme protein) of 0.0001% to 0.1%; With 0% to 5% micro constitutent (as foam inhibitor, spices).

3) be configured to a composition of detergent for particulate material, its bulk density is at least 600g/L, containing the linear alkylbenzene sulfonate (LAS) (by acid) of about 5% to about 9%; The alcohol ethoxylate of about 7% to about 14% is (as C _12-15alcohol, 7 EO unit); The fatty acid soaps of about 1 to about 3% is (as C _16-22aliphatic acid); Sodium carbonate (the Na of about 10% to about 17% ₂cO ₃); The soluble silicate of about 3% to about 9% is (as Na ₂o2SiO ₂); Zeolite (the NaA1SiO of about 23% to about 33% ₄); The sodium sulphate of 0% to about 4% is (as Na ₂sO ₄); The sodium perborate of about 8% to about 16% is (as NaBO ₃h ₂o); The TAED of about 2% to about 8%; The phosphonate (as EDTMPA) of 0% to about 1%; The carboxymethyl cellulose (CMC) of 0% to about 2%; The polymer (as maleic acid/acrylic copolymer, PVP, PEG) of 0% to 3%; The enzyme (by pure enzyme protein) of 0.0001% to 0.1%; With 0% to 5% micro constitutent (as foam inhibitor, spices, fluorescent whitening agent).

4) be configured to a composition of detergent for particulate material, its bulk density is at least 600g/L, containing the linear alkylbenzene sulfonate (LAS) (by acid) of about 8% to about 12%; The alcohol ethoxylate of about 10% to about 25% is (as C _12-15alcohol, 7 EO unit); Sodium carbonate (the Na of about 14% to about 22% ₂cO ₃); The soluble silicate of about 1% to about 5% is (as Na ₂o2SiO ₂); The zeolite of about 25% to about 35% is (as NaA1SiO ₄); The sodium sulphate of 0% to about 10% is (as Na ₂sO ₄); The carboxymethyl cellulose (CMC) of 0% to about 2%; The polymer (as maleic acid/acrylic copolymer, PVP, PEG) of 1% to 3%; The enzyme (by pure enzyme protein) of 0.0001% to 0.1%; With 0% to 5% micro constitutent (as foam inhibitor, spices).

5) aqueous liquid detergent compositions, containing the linear alkylbenzene sulfonate (LAS) (by acid) of about 15% to about 21%; The alcohol ethoxylate of about 12% to about 18% is (as C _12-15alcohol, 7 EO unit; Or C _12-15alcohol, 5 EO unit); The fatty acid soaps (as oleic acid) of about 3% to about 13%; Alkenyl succinic acid (the C of 0% to about 13% _12-14alkenyl succinic acid); The ethylaminoethanol of about 8% to about 18%; The citric acid of about 2% to about 8%; The phosphonate of 0% to about 3%; The polymer (as PVP, PEG) of 0% to about 3%; The borate of 0% to about 2% is (as B ₄o ₇); The ethanol of 0% to about 3%; The propane diols of about 8% to about 14%; The enzyme (by pure enzyme protein) of 0.0001% to 0.1%; With 0% to 5% micro constitutent (as dispersant, foam inhibitor, spices, fluorescent whitening agent).

6) a structural type aqueous liquid detergent compositions, containing the linear alkylbenzene sulfonate (LAS) (by acid) of about 15% to about 21%; The alcohol ethoxylate of 3% to 9% is (as C _12-15alcohol, 7 EO unit; Or C _12-15alcohol, 5 EO unit); The fatty acid soaps (as oleic acid soap) of about 3% to about 10%; Zeolite (the NaA1SiO of about 14% to about 22% ₄); The potassium citrate of about 9% to about 18%; The borate of 0% to about 2% is (as B ₄o ₇); The carboxymethyl cellulose (CMC) of 0% to about 2%; The polymer (as PEG, PVP) of 0% to about 3%; The grappling polymer (as lauryl methacrylate/acrylic copolymer, mol ratio 25:1, MW3800) of 0% to about 3%; The glycerine of 0% to about 5%; The enzyme (by pure enzyme protein) of 0.0001% to 0.1%; With 0% to 5% micro constitutent (as dispersant, foam inhibitor, spices, fluorescent whitening agent).

7) be configured to a composition of detergent for particulate material, its bulk density is at least 600g/L, containing the aliphatic alcohol sulfate of about 5% to about 10%; The ethoxylated fatty acid single ethanol amide of about 3% to about 9%; The fatty acid soaps of 0% to 3%; The sodium carbonate of about 5% to about 10% is (as Na ₂cO ₃); The soluble silicate of about 1% to about 4% is (as Na ₂o2SiO ₂); The zeolite of about 20% to about 40% is (as NaA1SiO ₄); The sodium sulphate of about 2% to about 8% is (as Na ₂sO ₄); The sodium perborate of about 12% to about 18% is (as NaBO ₃h ₂o); The TAED of about 2% to about 7%; The polymer (as maleic acid/acrylic copolymer, PEG) of about 1% to about 5%; The enzyme (by pure enzyme protein) of 0.0001 to 0.1%; With 0% to 5% micro constitutent (as fluorescent whitening agent, foam inhibitor, spices).

8) composition of detergent for particulate material is configured to, containing the linear alkylbenzene sulfonate (LAS) (by acid) of about 8% to about 14%; The ethoxylated fatty acid single ethanol amide of about 5% to about 11%; The fatty acid soaps of 0% to about 3%; The sodium carbonate of about 4% to about 10% is (as Na ₂cO ₃); Soluble silicate (the Na of about 1% to about 4% ₂o2SiO ₂); The zeolite of about 30% to about 50% is (as NaA1SiO ₄); The sodium sulphate of about 3% to about 11% is (as Na ₂sO ₄); The natrium citricum of about 5% to about 12% is (as C ₆h ₅na ₃o ₇); The polymer (as PVP, maleic acid/acrylic copolymer, PEG) of about 1% to about 5%; The enzyme (by pure enzyme protein) of 0.0001% to 0.1%; With 0% to 5% micro constitutent (as foam inhibitor, spices).

9) composition of detergent for particulate material is configured to, containing the linear alkylbenzene sulfonate (LAS) (by acid) of about 6% to about 12%; The nonionic surface active agent of about 1% to about 4%; The fatty acid soaps of about 2% to about 6%; The sodium carbonate of about 14% to about 22% is (as Na ₂cO ₃); The zeolite of about 18% to about 32% is (as NaA1SiO ₄); The sodium sulphate of about 5% to about 20% is (as Na ₂sO ₄); The natrium citricum of about 3% to about 8% is (as C ₆h ₅na ₃o ₇); The sodium perborate of about 4% to about 9% is (as NaBO ₃h ₂o); The bleach-activating (as NOBS or TAED) of about 1% to about 5%; The carboxymethyl cellulose (CMC) of 0% to about 2%; The polymer (as polycarboxylate or PEG) of about 1% to about 5%; The enzyme (by pure enzyme protein) of 0.0001% to 0.1%; With 0% to 5% micro constitutent (as fluorescent whitening agent, spices).

10) aqueous liquid detergent compositions, containing the linear alkylbenzene sulfonate (LAS) (by acid) of about 15% to about 23%; The alcohol ethyoxysulfates of about 8% to about 15% is (as C _12-15alcohol, 2 to 3 EO unit); The alcohol ethoxylate of about 3% to about 9% is (as C _12- ₁₅alcohol, 7 EO unit; Or C _12-15alcohol, 5 EO unit); The fatty acid soaps (as laurate soap) of 0% to about 3%; The ethylaminoethanol of about 1% to about 5%; The natrium citricum of about 5% to about 10%; The hydrotrote (as toluenesulfonic acid sodium salt) of about 2% to about 6%; The borate of 0% to about 2% is (as B ₄o ₇); The carboxymethyl cellulose of 0% to about 1%; The ethanol of about 1% to about 3%; The propane diols of about 2% to about 5%; The enzyme (by pure enzyme protein) of 0.0001% to 0.1%; With 0% to 5% micro constitutent (as polymer, dispersant, spices, fluorescent whitening agent).

11) aqueous liquid detergent compositions, containing the linear alkylbenzene sulfonate (LAS) (by acid) of about 20% to about 32%; The alcohol ethoxylate of 6% to 12% is (as C _12-15alcohol, 7 EO unit; Or C _12-15alcohol, 5 EO unit); The ethylaminoethanol of about 2% to about 6%; The citric acid of about 8% to about 14%; The borate of about 1% to about 3% is (as B ₄o ₇); The polymer (such as maleic acid/acrylic copolymer, grappling polymer is as lauryl methacrylate/acrylic copolymer) of 0% to about 3%; The glycerine of about 3% to about 8%; The enzyme (by pure enzyme protein) of 0.0001% to 0.1%; With 0% to 5% micro constitutent (as hydrotrote, dispersant, spices, fluorescent whitening agent).

12) a kind of composition of detergent being configured to particulate material, its bulk density is at least 600g/L, containing the anionic surfactant (linear alkylbenzene sulfonate (LAS), alkyl sulfate, alpha-alkene sulfonate, alpha-sulfo fatty acid methyl ester, paraffin sulfonate, soap) of about 25% to about 40%; The nonionic surface active agent (as alcohol ethoxylate) of about 1% to about 10%; The sodium carbonate of about 8% to about 25% is (as Na ₂cO ₃); The soluble silicate of about 5% to about 15% is (as Na ₂o2SiO ₂); The sodium sulphate of 0% to about 5% is (as Na ₂sO ₄); Zeolite (the NaA1SiO of about 15% to about 28% ₄); The sodium perborate of 0% to about 20% is (as NaBO ₃4H ₂o); The bleach-activating (TAED or NOBS) of about 0% to about 5%; The enzyme (by pure enzyme protein) of 0.0001% to 0.1%; With 0% to 3% micro constitutent (as spices, fluorescent whitening agent).

13) as the 1st above) to the 12nd) described by composition of detergent, wherein the linear alkylbenzene sulfonate (LAS) of all or part is by (C ₁₂-C ₁₈) alkyl sulfate substitute.

14) be configured to a composition of detergent for particulate material, its bulk density is at least 600g/L, containing (the C of about 9% to about 15% ₁₂-C ₁₈) alkyl sulfate; The alcohol ethoxylate of about 3% to about 6%; The polyhydroxy alkyl fatty acid acid amides of about 1% to about 5%; The zeolite of about 10% to about 20% is (as NaA1SiO ₄); The layered disilicate (as the SK56 that Hoechst produces) of about 10% to about 20%; The sodium carbonate of about 3% to about 12% is (as Na ₂cO ₃); The soluble silicate of 0% to about 6% is (as Na ₂o2SiO ₂); The natrium citricum of about 4% to about 8%; The SODIUM PERCARBONATE of about 13% to about 22%; The TAED of about 3% to about 8%; The polymer (as polycarboxylate and PVP) of 0% to about 5%; The enzyme (by pure enzyme protein) of 0.0001% to 0.1%; With 0% to 5% micro constitutent (as fluorescent whitening agent, photobleaching agent, spices, foam inhibitor).

15) be configured to a composition of detergent for particulate material, its bulk density is at least 600g/L, containing (the C of about 4% to about 8% ₁₂-C ₁₈) alkyl sulfate; The alcohol ethoxylate of about 11% to about 15%; The soap of about 1% to about 4%; The zeolite MAP of about 35% to about 45% or Wessalith CS; Sodium carbonate (the Na of about 2% to about 8% ₂cO ₃); The soluble silicate of 0% to about 4% is (as Na ₂o2SiO ₂); The SODIUM PERCARBONATE of about 13% to about 22%; The TAED of 1% to 8%; The carboxymethyl cellulose (CMC) of 0% to about 3%; The polymer (as polycarboxylate and PVP) of 0% to about 3%; The enzyme (by pure enzyme protein) of 0.0001% to 0.1%; With 0% to 3% micro constitutent (as fluorescent whitening agent, phosphonate, spices).

16) as the 1st above) to the 15th) detergent compositions described in item, containing peracid that is stable or encapsulating, this peracid is annexing ingredient, or for it before the substitute of bleach system of regulation.

17) as the 1st above), 3), 7), 9) and the 12nd) composition of detergent described in item, wherein perborate is substituted by percarbonate.

18) as the 1st above), 3), 7), 9), 12), 14) and the 15th) composition of detergent described in item, it is additionally containing Mn catalyst.Mn catalyst is such as " Efficientmanganesecatalystsforlow-temperaturebleaching " Nature369:637-639 (1994) (" Efficient manganese catalysts for cold bleaching ", " nature ", 369th volume, 637-639 page, 1994) the middle a kind of compound described.

19) composition of detergent for non-aqueous detergent liquid is configured to, containing liquid nonionic surfactant (as linear alkoxylated primary alconol), adjuvant system (as phosphate), one or more enzymes, and alkali.Washing agent also can comprise anionic surfactant and/or bleach system.

As mentioned above, enzyme of the present invention can be mixed with concentration conventional in washing agent.At present it is contemplated that the enzyme amount of adding in composition of detergent may correspond to often liter of cleaning solution add 0.00001 to 1.0mg enzyme polypeptide (by pure enzyme protein).

Composition of detergent also can comprise the detergent ingredients of other routines, as deflocculant, packing material, foam in hibitors, corrosion-resistant agent, soil suspender, chelating agent, anti-dirt precipitating reagent, dehydrating agent, dyestuff, bactericide, fluorescer, thickener and spices again.

Composition of detergent can be configured to hand washing (manually) or machine washing (automatically) laundry detergent composition, comprises the laundry additive composition and rinse added fabric softener composition that are applicable to the dirty fabric of pretreatment; Or the composition of detergent be configured to for average family hard surface cleaning operation; Or be formulated for dish operation manually or automatically.

Any one Cleasing compositions described herein all can comprise any multiple extra enzyme.In general, one or more enzymes above-mentioned should with the washing agent compatible (as relative to Optimal pH, compatible with other enzyme component and non-enzyme components etc.) selected, and these enzymes should add with effective dose.There is provided following enzyme exemplarily.

Protease: suitable protease comprises the protease coming from animal, plant or microorganism.In the protein of chemical modification mutant or protein engineering mutant and natural process is also included within.Protease can be serine protease or metalloproteinases, alkaline microbial protease, trypsin like proteases or chymotrypsin-like proteinase.The example of alkali protease is subtilopeptidase A, such as derive from those of bacillus, as subtilopeptidase A Novo, subtilopeptidase A Carlsberg, subtilopeptidase A 309, subtilopeptidase A 147 and subtilopeptidase A 168 (see such as WO89/06279).The example of trypsin like proteases is trypsase (such as coming from the trypsase of pig or ox) and Fusarium protease (see such as, WO89/06270 and WO94/25583).The example of available protease also includes but not limited to the variant described in WO92/19729, WO98/20115, WO98/20116 and WO98/34946.The protease of commercially available acquisition includes but not limited to: pRIMASE ^tM, DURALASE ^tM, kANNASE ^tMand BLAZE ^tM(NovoNordiskA/S and NovozymesA/S); mAXACAL ^tM, MAXAPEM ^tM, pURAFECTOXP ^tM, FN2 ^tMand FN3 ^tM(DaniscoUSInc.).Other Exemplary proteases comprise the NprE being derived from bacillus amyloliquefaciens and the ASP being derived from Cellulomonas strain 69B4.

Lipase: suitable lipase comprises the lipase coming from bacterium or fungi.Comprise the mutant of chemical modification, the mutant of proteolysis modification or protein engineering mutant.The example of available lipase includes but not limited to the lipase deriving from Humicola (synonym thermophilic fungal belongs to (Thermomyces)), such as derive from thin cotton like humicola lanuginosa (H.lanuginosa) (dredge the thermophilic hyphomycete of cotton like (T.lanuginosus)) (see such as, EP258068 and EP305216), derive from the lipase of Humicola insolens (H.insolens) (see such as, WO96/13580), pseudomonas (Pseudomonas) lipase, such as, derive from Pseudomonas alcaligenes (P.alcaligenes) or pseudomonas pseudoalcaligenes (P.pseudoalcaligenes) (see such as, EP218272), Pseudomonas cepacia (P.cepacia) is (see such as, EP331376), pseudomonas-stutzeri (P.stutzeri) is (see such as, GB1, 372, 034), Pseudomonas fluorescens (P.fluorescens), pseudomonas strain SD705 is (see such as, WO95/06720 and WO96/27002), Wisconsin pseudomonad (P.wisconsinensis) is (see such as, WO96/12012) lipase, bacillus lipase, such as derive from bacillus subtilis (see such as, Dartoisetal.BiochemicaetBiophysicaActa, 1131:253-360 (the 1993) (people such as Dartois, " Acta Biochimica et Biophysica Sinica ", 1131st volume, 253-360 page, 1993)), bacillus stearothermophilus is (see such as, or the lipase of bacillus pumilus (see such as, WO91/16422) JP64/744992).Imagination comprises those such as described in WO92/05249, WO94/01541, WO95/35381, WO96/00292, WO95/30744, WO94/25578, WO95/14783, WO95/22615, WO97/04079, WO97/07202, EP407225 and EP260105 for the extra lipase Variant in preparation.The lipase of some commercially available acquisitions comprises and LIPOLASEULTRA ^tM(NovoNordiskA/S and NovozymesA/S).

Polyester enzyme: suitable polyester enzyme can be comprised in composition, such as, described in WO01/34899, WO01/14629 and US6933140 those.

Amylase: said composition can mix with amylase, such as, can not improve the amylase of output.Described amylase can comprise the amylase of commercially available acquisition, such as but not limited to and BAN ^tM(NovoNordiskA/S and NovozymesA/S); with (deriving from DaniscoUSInc.).

Cellulase: cellulase can be added in composition.Suitable cellulase comprises the cellulase coming from bacterium or fungi.Also comprise mutant or the protein engineering mutant of chemical modification.Suitable cellulase comprises the cellulase deriving from bacillus, pseudomonas, Humicola, Fusarium, careless rhizopus (Thielavia), Acremonium (Acremonium), such as, by such as U.S. Patent No. 4,435,307, No.5,648,263, No.5,691,178, No.5,776,757 and WO89/09259 in disclosed Humicola insolens (Humicolainsolens), thermophilic fungus destroyed wire (Myceliophthorathermophila) and Fusarium oxysporum generation fungal cellulase.The exemplary fiber element enzyme that imagination uses is cellulase yarn fabric to color care benefits.The example of this type of cellulase is the cellulase such as described in EP0495257, EP0531372, WO96/11262, WO96/29397 and WO98/08940.Other examples are cellulase variants, such as WO94/07998, WO98/12307, WO95/24471, PCT/DK98/00299, EP531315, U.S. Patent No. 5,457,046, No.5,686,593 and No.5,763, and those described in 254.The cellulase of commercially available acquisition comprises with (NovoNordiskA/S and NovozymesA/S); (DaniscoUSInc.); And KAC-500 (B) ^tM(KaoCorporation).

Peroxidase/oxidizing ferment: the imagination suitable peroxidase/oxidizing ferment be used in composition comprise come from plant, bacterium or fungi those.Comprise mutant or the protein engineering mutant of chemical modification.The example of available peroxidase comprises and derives from Coprinus (Coprinus) described in WO93/24618, WO95/10602 and WO98/15257, such as derive from the peroxidase of Coprinus cinereus (C.cinereus), and variant.The peroxidase of commercially available acquisition comprises such as GUARDZYME ^tM(NovoNordiskA/S and NovozymesA/S).

Composition of detergent also can comprise 2,6-β-D-fructan-hydrolying enzyme, and the biomembrane that household and/or industrial textile thing/clothing exist effectively can be removed/be cleaned to this enzyme.

By adding the independent additive containing one or more enzymes, or by adding the additive package containing all these enzymes, detergent enzyme is contained in composition of detergent.Detergent additives, namely additive or additive package can be configured to such as particulate material, liquid, slurry etc. separately.Exemplary detergent additives preparation includes but not limited to particulate material (being non-dust granules specifically), liquid (liquid stable specifically) or slurry.

Also can be made into low-dust granules material, and add particulate composition of the present invention, such as U.S. Patent No. 4,106,991 and No.4,661, described in 452, and optionally use methods known in the art to carry out dressing to it.The example of waxy coating materi is PEO product (such as, polyethylene glycol, PEG), and average mol is 1,000 to 20,000; There is the ethoxylated nonylphenol of 16 to 50 ethylene oxide units; Ethoxylized fatty alcohol, wherein alcohol contains 12 to 20 carbon atoms, and wherein has 15 to 80 ethylene oxide units; Fatty alcohol; Aliphatic acid; And the monoglyceride of aliphatic acid, diglyceride and triglycerides.The example being suitable for the film-forming coating materials applying fluidization is listed in such as GB1483591.Such as, according to the method set up, liquid enzyme formulation stabilisation can be made by adding polyhydric alcohols such as propane diols, sugar or sugar alcohol, lactic acid or boric acid.

Composition of detergent can be arbitrarily form easily, such as, and bar-shaped, tablet, powder, particle, paste or liquid.Liquid detergent can be water-based, usually containing maximum water of about 70% and the organic solvent of 0% to about 30%.Also it is contemplated that the compact detergent gel containing have an appointment 30% or less water.Composition of detergent can optionally comprise one or more surfactants, and this surfactant can be nonionic, comprises semi-polarity and/or anionic and/or cationic and/or amphoteric ion type.Surfactant can about 0.1 % by weight to about 60 % by weight wide region exist.

When being included in wherein, washing agent is by the anionic surfactant generally containing about 1% to about 40%, such as linear alkylbenzene sulfonate (LAS), alpha-alkene sulfonate, alkyl sulfate (aliphatic alcohol sulfate), alcohol ethyoxysulfates, secondary paraffin sulfonate, alpha-sulfo fatty acid methyl ester, alkyl or alkenyl butanedioic acid, or soap.

When being included in wherein; washing agent will generally contain the nonionic surface active agent of about 0.2% to about 40%, such as the N-acyl N-alkyl derivatives (" glucamide ") of alcohol ethoxylate, nonyl phenol ethoxylate, alkyl polyglycoside, alkyl dimethyl amine oxide, ethoxylated fatty acid single ethanol amide, fatty monoethanol amide, polyhydroxy alkyl fatty acid acid amides or aminoglucose.

Composition of detergent can comprise detergent builders or the complexing agent of 0% to about 65%, such as zeolite, diphosphate, triphosphate, phosphonate, carbonate, citrate, NTA, ethylenediamine tetra-acetic acid (EDTA), diethylene-triamine pentaacetic acid, alkyl or alkenyl butanedioic acid, soluble silicate or phyllosilicate (e.g., the SKS-6 of Hoechst product).

Washing agent can comprise one or more polymer.Illustrative polymers draws together carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), polyvinyl alcohol (PVA), poly-(vinylpyridine-N-oxide), poly-(vinyl imidazole), polycarboxylate as polyacrylate, maleic acid/acrylic copolymer, and lauryl methacrylate/acrylic copolymer.

The enzyme in composition of detergent stablized by the stabilizing agent of available routine, and these stabilizing agents are such as polyalcohol (as propane diols or glycerine); Sugar or sugar alcohol; Lactic acid; Boric acid or boronic acid derivatives (as aromatic borate); Or phenyl boronic acid derivative (as 4-formyl phenylboronic acid).Said composition method can be prepared according to WO92/19709 and WO92/19708.

Expect in the composition of detergent containing enzyme, can add according to following respective amount: add about 0.01 to about 100mg zymoprotein in often liter of wash liquid (such as, add about 0.05 to about 5.0mg zymoprotein in often liter of wash liquid, or in often liter of wash liquid, add 0.1 to about 1.0mg zymoprotein).

Although the compositions and methods of the invention are described in conjunction with following details, should be appreciated that and can make various amendment to it.

It is known in the art that many enzymes clean determination method, comprises sample and microsize sample mensuration.

brewage composition

Particulate composition of the present invention can be the component brewageing composition used in the technique providing fermented beverage (as beer).It is believed that fermentable carbohydrate does not form the most of dissolved solid in final beer.The reservation of these remnants is because malto-amylase can not amylatic α-1,6 key.Fermentable carbohydrate is not every 12 ounces (about 340 grams) beer contributions about 50 calories.Particulate composition of the present invention is usually combined with glucoamylase and optional amylopectase and/or isoamylase, contributes to starch to be converted to dextrin and fermentable sugars, reduces the remnants not fermentable carbohydrate in final beer.

Making these beverages primary raw material used is water, hops and Fructus Hordei Germinatus.In addition, but exclusively, such as conventional corn crushed grain, refining hominy grits, beer are milled yeast, paddy rice, Chinese sorghum, refining cornstarch, barley, barley starch, hulled barley, wheat, wheaten starch, baking cereal, cereal flake, rye, oat, potato, tapioca and syrup, as the adjuncts such as corn syrup, sugarcane syrup, invert syrup, barley and/or wheat syrup can be used as the source of starch.

Due to some reasons, the Fructus Hordei Germinatus made primarily of selected barley variety plays an important role for the bulk property of beer and quality.The first, Fructus Hordei Germinatus is the main flavor enhancement of beer.The second, Fructus Hordei Germinatus provides the fermentable sugars of the overwhelming majority.3rd, Fructus Hordei Germinatus provides protein, can affect wine body and the foam characteristic of beer.4th, Fructus Hordei Germinatus provides necessary enzymatic activity in mashing process.Hops is also to beer quality, and comprising taste has remarkable impact.Specifically, hops (or hop components) adds required bitter substance in beer.In addition, hops can be used as protein precipitant, forms anticorrisive agent and contributes to the formation of foam and stablize.

Cereal (grain) is as barley, oat, wheat, and corn and paddy rice are generally used for industry and family brewages, in addition also interpolation other plant component usually, such as hops.The component brewageing middle use can not germinateed, and can be to germinate yet, and namely part is sprouted, thus causes the level of enzyme to increase, and comprises AMS.For realizing successfully brewageing, enough AMS enzyme running water is needed to put down, to guarantee there is the sugar of proper level for fermentation.Also particulate composition of the present invention can be added in the component for brewageing.

As used herein, term " raw material " refers to crushed or broken cereal and plant component.Such as, barley used in Beer Brewage has been roughly ground or the particle of fragmentation, to obtain the denseness being applicable to prepare fermentation wine with dregs.As used herein, term " raw material " comprises the plant of being pulverized or roughly grinding form and the cereal of any the above-mentioned type.Method as herein described can be used for measuring the alpha-amylase activity level in flour and raw material.

The technique preparing beer is known in the art.See such as, WolfgangKunze (2004) " TechnologyBrewingandMalting; " ResearchandTeachingInstituteofBrewing, Berlin (VLB), 3rdedition (WolfgangKunze, " Fructus Hordei Germinatus makes and brewing technique " in 2004, Berlin brewing institute (VLB), the third edition).In brief, this technique relates to: (a) prepares wine with dregs, and (b) filters wine with dregs to prepare brewer's wort, and (c) fermenting wort is to obtain fermented beverage, such as beer.Usually, by the Fructus Hordei Germinatus of milling or pulverize, Fructus Hordei Germinatus and auxiliary agent or auxiliary agent and water is mixed be incorporated in controllable temperature under keep a period of time, make the enzyme existed in Fructus Hordei Germinatus and/or auxiliary agent be fermentable sugars by the Starch Conversion existed in Fructus Hordei Germinatus.Then wine with dregs is transferred in wine with dregs filter, liquid is separated with cereal remnants.This sweet liquid is called as " brewer's wort ", and remaining cereal remnants are called as " vinasse ".Usually will extract wine with dregs, it relates to the soluble extract adding water to reclaim remnants in vinasse in wine with dregs.Then brewer's wort is fully boiled, to help adjustable colors, taste and smell to brewer's wort sterilizing.The a certain moment in boiling part adds hops.After brewer's wort cooling, transferred in fermentation tank.

Then brewer's wort contacts with yeast in fermentation tank.Fermentation tank can cool to stop fermentation.The yeast that removing may be flocculated.Finally, cooled by beer and preserve a period of time, during this period of time beer becomes clarification, and its taste is formed, and may damage beer looks, taste and any precipitation of material of shelf-life out.Beer usually containing have an appointment 2% to about 10%v/v alcohol, but also can obtain the higher beer of alcohol content, such as 18%v/v.Before packaging, beer carbonation also is optionally carried out filtering and pasteurization.

For usually but not necessarily combine with one or more exogenous enzymes (such as glucoamylase, amylopectase and/or isoamylase and their any combination) comprise AMS brewage composition, can be added in the wine with dregs of previous step (a) (namely in the preparation process of wine with dregs).Alternatively or in addition, can add to brewageing composition in the wine with dregs of previous step (b) (such as in the process of filtering wine with dregs).Alternatively or in addition, can add to brewageing composition in the brewer's wort of previous step (c) (such as in the process of fermenting wort).

Specific embodiments relates to any such use, method or fermented beverage, wherein said fermented beverage is beer, such as malt beer, the beer that " purifying method " is brewageed, malt liquor, India's lager, lager beer, bitter, sparkling wine (the second beer), 3rd beer, dry beer, lager, light beer, low alcohol beer, low-heat beer, dark beer, bock, stout beer, Fructus Hordei Germinatus stout, alcohol-free beer, without alcohol malt liquor etc., and other cereals and malt beverage, such as fruity malt beverage, as oranges and tangerines taste, as lemon, orange taste, lime flavored or berry taste malt beverage, vinosity malt beverage, as vodka, Lang Mu or American aloe taste malt beverage, or caf malt beverage, as caffeine taste malt liquor, etc..

other animal feed embodiments

In some embodiments, particularly in the background of animal feed application, the present invention instructs the particle of content jointly can to use with at least one in following enzyme:

zytase

amylase

protease

phytase

cellulase

other

Only for example, one or more one-tenth that the feed of chicken (as broiler chicken) can be listed by following table in the stage % that such as following table provides are grouped into:

Composition	Brood (%)	Be bred as (%)
			Corn	46.2	46.7
Wheat bran	6.7	10.0
			Corn DDGS	7.0	7.0
Dregs of beans 48%CP	32.8	26.2
			Animal/vegetable fat blend	3.0	5.8
1B HCl	0.3	0.3
			Dl-methione	0.3	0.3
L-threonine	0.1	0.1
			Salt	0.3	0.4
Lime stone	1.1	1.1
			Dicalcium Phosphate	1.2	1.2
Poultry vitamin and trace mineral	0.3	0.3

Only for example, the dietary standards of chicken (as broiler chicken) can be as shown in the table:

Dietary standards
			Crude protein (%)	23.00	20.40
Poultry metabolizable energy (kcal/kg)	2950	3100
			Calcium (%)	0.85	0.85
Available phosphorus (%)	0.38	0.38
			Sodium (%)	0.18	0.19
Apparent digestible lysine (%)	1.21	1.07
			Methionine (%) can be digested	0.62	0.57
Methionine+cysteine (%) can be digested	0.86	0.78
			Threonine (%) can be digested	0.76	0.68

Only for example, one or more one-tenth that the feed of laying hen can be listed by following table in the stage % that such as following table provides are grouped into:

Only for example, the dietary standards of laying hen can be as shown in the table:

Dietary standards
		Crude protein (%)	16.10
Poultry metabolizable energy (kcal/kg)	2700
		Lysine (%)	0.85
Methionine (%)	0.42
		Methionine+cysteine (%)	0.71
Threonine (%)	0.60
		Calcium (%)	3.85
Available phosphorus (%)	0.42
		Sodium (%)	0.16

Only for example, one or more one-tenth that the feed of turkey can be listed by following table in the stage % that such as following table provides are grouped into:

Composition	Stage 1 (%)	Stage 2 (%)	Stage 3 (%)	Stage 4 (%)
					Wheat	33.6	42.3	52.4	61.6
Corn DDGS	7.0	7.0	7.0	7.0
					Dregs of beans 48%CP	44.6	36.6	27.2	19.2
Rapeseed dregs	4.0	4.0	4.0	4.0
					Soybean oil	4.4	4.2	3.9	3.6
1B HCl	0.5	0.5	0.4	0.4
					Dl-methione	0.4	0.4	0.3	0.2
L-threonine	0.2	0.2	0.1	0.1
					Salt	0.3	0.3	0.3	0.3
Lime stone	1.0	1.1	1.1	1.0
					Dicalcium Phosphate	3.5	3.0	2.7	2.0
Poultry vitamin and trace mineral	0.4	0.4	0.4	0.4

Only for example, the dietary standards of turkey can be as shown in the table:

Dietary standards
					Crude protein (%)	29.35	26.37	22.93	20.00
Poultry metabolizable energy (kcal/kg)	2.850	2.900	2.950	3.001
					Calcium (%)	1.43	1.33	1.22	1.02
Available phosphorus (%)	0.80	0.71	0.65	0.53
					Sodium (%)	0.16	0.17	0.17	0.17
Apparent digestible lysine (%)	1.77	1.53	1.27	1.04
					Methionine (%) can be digested	0.79	0.71	0.62	0.48
Methionine+cysteine (%) can be digested	1.12	1.02	0.90	0.74
					Threonine (%) can be digested	1.03	0.89	0.73	0.59

Only for example, one or more one-tenth that the feed of piglet can be listed by following table in the stage % that such as following table provides are grouped into:

Composition	Stage 1 (%)	Stage 2 (%)
			Corn	20.0	7.0
Wheat	25.9	46.6
			Rye	4.0	10.0
Wheat bran	4.0	4.0
			Corn DDGS	6.0	8.0
Dregs of beans 48%CP	25.7	19.9
			Whey powder	10.0	0.0
Soybean oil	1.0	0.7
			1B HCl	0.4	0.5
Dl-methione	0.2	0.2
			L-threonine	0.1	0.2
L-Trp	0.03	0.04
			Lime stone	0.6	0.7
Dicalcium Phosphate	1.6	1.6
			Pig vitamin and trace mineral	0.2	0.2
Salt	0.2	0.4

Only for example, the dietary standards of piglet can be as shown in the table:

Only for example, one or more one-tenth that the feed of grower pigs/growing and fattening pigs can be listed by following table in the stage % that such as following table provides are grouped into:

Composition	Grow/fatten (%)
		Corn	27.5
Dregs of beans 48%CP	15.4
		Corn DDGS	20.0
Wheat bran	11.1
		Rice bran	12.0
The canola oil dish dish dregs of rice	10.0
		Lime stone	1.6
Dicalcium Phosphate	0.01
		Salt	0.4
Pig vitamin and trace mineral	0.3
		Lysine-HCl	0.2
Vegetable oil	0.5

Only for example, the dietary standards of grower pigs/growing and fattening pigs can be as shown in the table:

Dietary standards
		Crude protein (%)	22.60
Pig metabolizable energy (kcal/kg)	3030
		Calcium (%)	0.75
Available phosphorus (%)	0.29
		Apparent digestible lysine (%)	1.01
Methionine+cysteine (%) can be digested	0.73
		Threonine (%) can be digested	0.66

dosing

Single feed can be designed to by according to feed addictive composition of the present invention, also can be designed to feed every day.

Optimised quantity in conjunction with the composition (and wherein each component) of the present invention's use will depend on the method that pending product and/or product contact with composition and/or its desired use.

The amount of the enzyme used in composition should be q.s, effectively and fully efficiently could keep the performance improvement to the animal feed product comprising described composition like this.This validity duration should extend to the time at least utilized when product (such as feed addictive composition or comprise its feed).

be combined with other components in feed applications

Enzyme as herein described can be combined with other components.

This component can be prebiotics.Prebiotics be generally Non-digestible carbohydrates (oligosaccharides or polysaccharide) or in UGI non-degradable or absorb sugar alcohol.In commercial product used and according to the present invention can known prebiotics comprise inulin (FOS or FOS) and transgalactooligosac,harides (GOS or TOS).(namely suitable prebiotics comprises iMOS, soyabean oligosaccharides, alginates, xanthans, pectin, locust bean gum (LBG), inulin, guar gum, galactooligosaccharide (GOS), FOS (FOS), nondegradable starch, lactosucrose, lactulose, lactitol, maltitol, maltodextrin, dextrosan ), lactitol, lactosucrose, soyabean oligosaccharides, isomaltoketose, isomalto-oligosaccharide, glucose oligosaccharide and xylo-oligosaccharide.

Prebiotics can be used with feed addictive composition according to the present invention (or its component) simultaneously (such as admixed together with it or sent by identical or different approach) simultaneously, also can send in order with it (such as by identical or different approach).

Other components of combination of the present invention comprise dextrosan (as ) and/or maltodextrin and/or lactitol.These other components optionally can be added in feed addictive composition, to assist dry run.

The other example of other suitable components comprise following in one or more: thickener, gelling agent, emulsifying agent, adhesive, crystal modification agent, sweetener (comprising artificial sweetening agent), rheology modifier, stabilizing agent, antioxidant, dyestuff, enzyme, carrier, carrier, excipient, diluent, lubricant, flavor enhancement, coloring material, suspending agent, disintegrant, granulation binders etc.These other components can be natural.These other components are prepared by using chemistry and/or zymotechnic.

In a preferred embodiment, the enzyme that the present invention uses can use with one or more lipid bindings.

Such as, the enzyme that the present invention uses can be combined with one or more lipid micelles.This lipid micelles can be simple lipid micelle or complex lipids micelle.

This lipid micelles may be the aggregation of the oriented molecule of amphiphilic species, such as lipid and/or oil.

Instruction content of the present invention still further provides a kind of method increasing experimenter (i.e. poultry or pig) body weight, comprises and will comprise according to the forage feed of feed addictive composition of the present invention to described experimenter.

By in conjunction with the following examples, can understand the present invention further, the mode that these embodiments illustrate by way of example provides, and is not intended to restriction.

embodiment:

embodiment 1

the size distribution of sodium sulphate core and smoothness number

Seven kinds of anhydrous crystal sodium sulphate core particle samples are obtained: MineradeSantaMarta (Villarubio, Spain) and Saltex (ForthWorth, Texas) from following two suppliers.

MalvernMastersizer2000 laser diffraction granularity analyzer is used to be drawn the size distribution of sample by laser light scattering.The algorithm of use described in " method " part and the real density (2.664g/cm of anhydrous sodium sulfate ³), calculate the covering specific area of these size distribution.The quick porosity of MicromeriticsASAP2420 type described in " method " part and Surface Area Analyzer is used to measure BET specific surface area.Calculate the ratio of these two specific areas, obtain the smoothness number of each sample.The presentation graphics of two batches of cores as shown in Figure 1.

table 1:

embodiment 2

use sodium sulphate core making detergent enzyme granulate sample

To load in the pilot-scale fluidized bed coater of 150kg batch size purchased from the 79.8kg sodium sulphate core of MineradeSantaMarta (the MSM lot number 3 in the table 1 of embodiment 1).By light scattering, the mass median diameter (D of these cores ₅₀) be 258 microns, D10 is 182 microns, and D90 is 358 microns; By screening, D ₅₀it is 210 microns.Based on this point, the PSDI of MSM lot number 3 is 362/182=1.99.

According to the table 1 of embodiment 1, the smoothness number of MSM lot number 3 core is 1.1, with the smoothness number 1.0 of perfectly smooth particle closely.The optical microscope photograph of core and electron scanning micrograph are as shown in Figure 1.Can find out in same figure, these cores are more more smooth than the comparative sample purchased from the core of Saltex.The smoothness number that table 1 shows Saltex lot number 1 is 3.9, shows to have remarkable deviation with complete smoothness number 1.0.

Core carries out fluidisation under the fluidized-bed temperature of 41 DEG C, the aqueous solution of protease (Purafast) being 20%w/w solid (3450PU/g) by the concentration of the partial hydrolysis polyvinyl alcohol (the final supported solid %w/w of enzyme/PVA is 16%) containing 1%w/w and 0.5%w/w defoamer (polyethylene glycol EP436E (lot number 1L05091501)) is sprayed onto on core, and atomization air pressure rises to 3.9 bar (finally) from 3.5 bar (initially) gradient.

After enzyme has sprayed, by the spray solution of the mixture containing sodium sulphate and magnesium sulfate (MgSO47H20,30.5%, Na2SO4,15%) on core, make its clean 20%w/w accounting for core (based on final particle weight).Under 45 DEG C of fluidized-bed temperatures, spray salting liquid with the atomization air pressure of 3.5 bar.

The final Coating Solution be made up of the polyvinyl alcohol (Celvol5-88) of partial hydrolysis, titanium dioxide and Lutensol nonionic surface active agent is sprayed onto on the coated enzyme core of salt, makes it account for the clean 11%w/w of total coated weight.Under 55 DEG C of fluidized-bed temperatures, to spray Coating Solution from the atomization air pressure of initial 5.3 bar Gradient Descent to 4.8 bar.

After this batch of coated granule cooling, gather in the crops 150kg product from fluidized bed coater.The D50 of final particle is 308 μm (passing through sieve analysis), and bulk density is 1.18g/cm3.The Hunter whiteness L value of this particle is the total dust of 73, Heubach enzyme is 3.2mg/ sheet.

embodiment 3

According to the formula described in embodiment 2 and method, only change the amount of the Purafast enzyme concentrated solution be coated on core, core granularity and also regulate core carrying capacity to adapt to the enzyme solid of varying level as required, thus prepare other four batches of Purafast particles.Do not change other dressing and process parameter.

Under different " pressure " conditions of storage, following enzyme sample is placed in clothing powder detergent and carries out storage test.Its stability is assessed by performance and biochemical analysis.

store test: sample preparation

The enzyme (vide infra) of corresponding amount to be added in 10g laundry detergent compositions and to put in plastic cup (150mL), openly in 37,45 and 60 DEG C of domestication rooms under 70% relative humidity to store.

A.10g clothing powder detergent+sample A1

B.10g clothing powder detergent+sample B1

C.10g clothing powder detergent+sample B2

D.10g clothing powder detergent+sample C1

E.10g clothing powder detergent+sample C2

When starting, store after 1 hour, after 2 hours, after 3 days (60 DEG C); And after 1 day, 3 days and 8 days (37 DEG C and 45 DEG C), sample is analyzed.

performance Evaluation

30 DEG C, under 25FH, in so-called Laundr-O-meter, carry out washing test 15 minutes.

The sample that 10g stores is dissolved in 2.5 liter of 25 ℉ H water (vide infra) in beaker, stirs 2 minutes.This solution of 300mL is put into LOM beaker, and adds following spot, to obtain the ratio L/C of liquid and cloth for about 90 (without steel balls).

Prepare 25L25 ℉ H=250ppmCa ^{+ 2}/ Mg ^{+ 2}=2/1, use 24583.25mL deionized water dilution 416.75mL15.000ppm stoste (to prepare 25 liters) stoste 15.000ppm:14.70 gram CaCl2.2H2O+10.165 gram MgCl2.6H2O, be dissolved in 1 liter of deionized water.

Spot in each beaker (initial reflectance [L*a*b, D65 working flare] of the sample before using reflectometer to measure:

After washing spot, rinse 3 minutes (17FH, Ca/Mg=4/1) under flowing tap water, Rotary drying also spends the night air-dry (using dark cloth to cover) at ambient conditions.

By using the final reflectivity [L*a*b, D65 irradiation body] of reflectometry three samples.Calculate δ dirt according to following formula and remove % (dE%):

Wherein L _reference=96.0

A _reference=0.55

B _reference=1.95

biochemistry is assessed

This method for measure Properase to fermentation, reclaim and the proteolytic activity of final products sample (liquid and particle).The method is colorimetric method, and monitors the degradation rate of N-succinyl-alanyl-alanyl-prolyl-phenylalanyl-paranitroanilinum substrate.Konelab analyzer is used to measure the release of the paranitroanilinum of substrate at 405nm place.By the standard of specifying, this determination method is calibrated.

sample preparation:

-by 100gTris buffer solution (0.1MTRIS, 0.01MCaCL ₂, 0.005%TritonX-100, pH8.6) to add in 10g washing agent sample and to stir 30min.Get the previous leachable of 2mL afterwards in microcentrifugal tube, with the centrifugal 10min of 14000rpm.

-use HAMILTON dilution machine and Tris buffer solution to prepare the second leachable (10X).

Vortex sample after-dilution, and be poured in corresponding Konelab colorimetric cylinder.Contrast (in these cases, PU/g) is set equally.Colorimetric cylinder is placed on specimen holder.By in this specimen holder inserting instrument.

Run Konelab to analyze:

-preparation work substrate solution [N-succinyl-alanyl-alanyl-prolyl-phenylalanyl-paranitroanilinum (AAPF) (Sigma article No. S7388)] is also poured in reagent container, be placed in (substrate=20mL container, code=AAPF) on reagent rotating disk.

prepared by reagent

1. substrate solution stoste (100mg/mL): 500mgAAPF is dissolved in 5mLDMSO.At room temperature keep in Dark Place.If solution becomes yellow, then abandon this solution.Stablize 1 month.

2. middle substrate solution (20mg/mL): add 800 μ L substrate solution stostes and mix in 3.2mLDMSO.At room temperature keep in Dark Place.If solution becomes yellow, then abandon this solution.Stablize 1 month.

3. work substrate solution (1mg/mL): in 4.75mL analysis buffer, add substrate solution in the middle of 250 μ L and mix.Prepare as required.If preserved on ice, usually stablize 1 hour.If solution becomes yellow, then abandon this solution.

-mark specimen cup in software also to define the test (six are repeated sample) that will run in Konelab.Run and check calibration.The activity (proportion of this formula supposition stripping mixture is 1g=1mL) of count particles sample:

PU/g particle=[(PU/mL) × (Working dilutions)] × [stripping buffer solution weight+particle (mL)/particle weight (g)]

-calculate the mean value of the repetition sample of each sample.Calculate CV% (coefficient of variation).

In this embodiment, " miniature " particle refers to the particle comprising the small light slide core of instructing content according to the present invention.

Data are shown in Figure 2.

embodiment 4

the preparation of three kinds of representative batch phytases

To load in the pilot-scale fluidized bed coater of 150kg batch size purchased from the 45kg sodium sulphate core of MineradeSantaMarta (MSM lot number 41418.B).By light scattering, the mass median diameter of core is 199 microns.

Core is carried out fluidisation, by the partial hydrolysis polyvinyl alcohol (Celvol5-88) containing 2%w/w and 0.5%w/w sodium phytate solution (Dr.Straetmans; DermofeelPA-3; Lot number NA2062) concentration be that the phytase aqueous solution (batch 488115613) of 17.5%w/w solid is sprayed onto on core.

After spraying enzyme, by the spray solution containing sodium sulphate (MSM lot number 13130405) on core, it is made to account for the clean 50%w/w of final particle weight.

The final Coating Solution that polyvinyl alcohol (Celvol5-88) and talcum (MicrotalcFCCG-AW, lot number 13/00328) by partial hydrolysis forms is sprayed onto on the coated enzyme core of salt, makes it account for clean 9% of total coated weight.

After each batch of coated granule cooling, gather in the crops 136kg, 140kg and 134kg product from fluidized bed coater.By light scattering, the D50 average out to of final particle 362 μm, bulk density is 1,27g/cm3.

Fig. 4 shows the granulation result of 3 kinds of representative particle batches 3115,3116 and 3117.Granulation test is carried out at DanishTeknologicalInstitute.

embodiment 5

three kinds of representative batch phytase standard TPT particles from identical concentration batches with pilot-scale produce

To load in the pilot-scale fluidized bed coater of 150kg batch size purchased from the 65kg sodium sulphate core of MineradeSantaMarta (MSMNa-G.III-EE, lot number 41235,12).By light scattering, the mass median diameter of core is generally 290 microns.

the portioned product specification of table 3:MSMNa-G.IIIEE

After spraying enzyme, by the spray solution containing sodium sulphate (MSM lot number 13130405) on core, it is made to account for the clean 40%w/w of final particle weight.

The final Coating Solution that polyvinyl alcohol (Celvol5-88) and talcum (MicrotalcFCCG-AW, lot number 13/00328) by partial hydrolysis forms is sprayed onto on the coated enzyme core of salt, makes it account for the clean 9%w/w of total coated weight.

After each batch of coated granule cooling, gather in the crops 151kg, 151kg and 150kg product from fluidized bed coater.By light scattering, the D50 average out to of final particle 388 μm, bulk density is 1.39g/cm3.

The granulation result of three kinds representative phytase standard TPT batch is shown in Figure 5.

Claims

1. a particle swarm, wherein said particle comprises:

Small light slide core particle, and

Activating agent,

The described small light slide core particle be wherein included in described group has:

Be less than the smoothness number of 3,

Be less than the mass median diameter of 300 microns,

Be less than the particle size distribution degree index of 2.5.

2. particle swarm according to claim 1, wherein said activating agent is enzyme.

3., according to particle swarm in any one of the preceding claims wherein, wherein said activating agent is the activating agent dressing around described smooth core particle.

4., according to particle swarm in any one of the preceding claims wherein, described particle swarm also comprises at least one deck additional packets clothing.

5. according to particle swarm in any one of the preceding claims wherein, described particle swarm also comprises at least one deck additional packets clothing, and the every one deck in wherein said at least one deck additional packets clothing accounts at least 5%w/w of described final particle.

6., according to particle swarm in any one of the preceding claims wherein, wherein said small light slide core particle comprises salt crystal.

7., according to particle swarm in any one of the preceding claims wherein, wherein said small light slide core particle comprises sodium sulfate crystal.

8. according to particle swarm in any one of the preceding claims wherein, wherein said activating agent comprises enzyme, and wherein said enzyme is at least one in phytase, protease or amylase.

9., according to particle swarm in any one of the preceding claims wherein, wherein said small light slide core particle has:

Be less than 2.8, be less than 2.5 or be less than 2 smoothness number;

Be less than 250 microns, be less than 225 microns, be less than 200 microns, be less than 175 microns, be less than 150 microns, be less than 125 microns or be less than the mass median diameter of 100 microns; And

Be less than 2.5, be less than 2 or be less than 1.5 particle size distribution degree index.

10. according to particle swarm in any one of the preceding claims wherein, described particle swarm also comprises at least one deck additional packets clothing, and the every one deck in wherein said at least one deck additional packets clothing accounts at least 6%, 7%, 8%, 9% or 10%w/w of described final particle.

11. 1 kinds of particle swarms, described particle swarm comprises:

Small light slide core particle, wherein said small light slide core particle has

Be less than 2.5 but be not less than 1.25 smoothness number,

Be less than 250 microns but be not less than 100 microns mass median diameter,

Be less than 2.5 but be not less than 1.5 particle size distribution degree index;

Comprise the activating agent dressing of enzyme, wherein said enzyme is at least one in phytase, protease or amylase; And

At least one deck additional packets clothing, the every one deck in wherein said at least one deck additional packets clothing accounts at least 7% of described final particle but is no more than 10%w/w.

12. according to particle swarm in any one of the preceding claims wherein, and described particle swarm also comprises:

First waterproof dressing, described first waterproof dressing comprises waterproof material; And

Around the moisturizing dressing of described first waterproof dressing, described moisturizing dressing comprises moisturizing material.

13. according to particle swarm in any one of the preceding claims wherein, and wherein said waterproof material is selected from barrier polymer, protein, lipid, fat and oil, aliphatic acid and natural gum.

14. according to particle swarm in any one of the preceding claims wherein, and wherein said moisturizing material is selected from starch, inorganic salts and carbohydrate.

15. 1 kinds of animal feed pellets, described animal feed pellet comprises the particle swarm according to any one of claim 1 to 14.

16. 1 kinds of non-granulated mixture of animal feed, the non-granulated mixture of described animal feed comprises the particle swarm according to any one of claim 1 to 14.

17. 1 kinds of laundry detergent compositions, described laundry detergent composition comprises the particle swarm according to any one of claim 1 to 14.

18. 1 kinds of dish washing detergent compositions, described dish washing detergent compositions comprises the particle swarm according to any one of claim 1 to 14.

19. 1 kinds of yarn fabric treatment compositions, described yarn fabric treatment compositions comprises the particle swarm according to any one of claim 1 to 14.

20. 1 kinds are cured composition, described in cure composition and comprise particle swarm according to any one of claim 1 to 14.

21. prepare a method for particle swarm, described method comprises:

With the activating agent coatings small light slide core particle containing enzyme, wherein said small light slide core particle has:

Be less than 2.5 but be not less than 1.25 smoothness number,

Be less than 300 microns but be not less than the mass median diameter of 150 microns, and

Be less than 3.0 but be not less than 1.5 particle size distribution degree index.

22. for the preparation of a method for animal feed composition, described method comprises:

The particle swarm of preparation according to any one of claim 1 to 14;

Described particle and feedstuff are mixed, obtains non-granulated mixture; And

At the temperature of 70 DEG C-95 DEG C, granulation is carried out to described non-granulated mixture.

23. 1 kinds of methods preparing feed addictive particle swarm, described method comprises:

The particle swarm of preparation according to any one of claim 1 to 14,

By described particle and feed acceptable carrier, diluent or mixed with excipients,

And (optionally) packaging.

24. application of particle swarm in steam treatment or granulation process according to any one of claim 1 to 14.

25. 1 kinds of methods improving storage stability, described method comprises the particle of preparation according to any one of claim 1 to 14, and is compared by the representative sample of storage stability with the particle swarm lacking small light slide core particle.

26. 1 kinds of methods reducing dust, described method comprises the particle of preparation according to any one of claim 1 to 14, and is compared by the representative sample of dust level with the particle swarm lacking small light slide core particle.