BR112015031623B1 - GRAIN POPULATION WITH SMALL SMOOTH CORES, ANIMAL FEED PELLET OR UNPELLETED ANIMAL FEED MIXTURE, DETERGENT CLOTHING WASH COMPOSITION, DETERGENT DISHES COMPOSITION, FABRIC TREATMENT COMPOSITION OR COMPOSITION FOR A FABRICATION OR COMPOSITION FOR A FURNITURE OR COMPOSITION FOR A FURNITURE OR FURNITURE FOR A FARMHOUSE. GRANULES, PROCESS FOR PRODUCTION OF ANIMAL FEED COMPOSITION, METHOD OF PREPARING A ADDITIVE GRAIN POPULATION POPULATION, USE OF A GRAIN POPULATION AND METHODS TO IMPROVE STORAGE STABILITY AND TO REDUCE DUST - Google Patents

Abstract

granules with small smooth cores. the present teachings provide an improved granule population comprising small smooth-core particles. methods of preparation and use are also provided.

Description

CROSS REFERENCE TO RELATED REQUESTS

[001] This application claims the benefit of the priority of provisional application No. U.S. USSN 61 / 837.122, filed on June 19, 2013, which is fully incorporated herein by reference.

FIELD OF THE INVENTION

[002] The present disclosure is aimed at improved populations of granules containing active agents and methods to produce and use.

BACKGROUND OF THE INVENTION

[003] The use of active agents, such as enzymes, in dry product formulations in industrial and consumer applications, such as detergents, textile compositions, bakery, food and animal feed, has become a common practice. Enzymes are known to break down dyes, modify fabric colors and textures, modify the viscosity of pasta and food, and optimize the digestibility of food and animal feed, by improving the availability of nutrients, such as soluble phosphate, and reducing anti-factors. -nutritional, such as phytic acid, in food and animal feed, thus improving the animal's productivity.

[004] Enzyme inactivation can occur during storage in dry product formulations, such as washing powder and dishwashing detergents, textile processing mixes, bread flours, and animal feed made up of ingredients such as grains (such as corn) or soy), vitamins, minerals and nutrients, such as choline chloride and, additionally, during industrial processing, (such as steam pelletizing of animal feed) by exposure to high temperatures, steam or high humidity, compression or shear stress, and chemical products (such as acids, bases, surfactants, bleaches or organic solvents). Inactivation is at least partially reversible if the enzyme reactivates after processing, for example, by cooling after steaming and pelletizing; however, inactivation is often irreversible, such that the catalytic activity of the enzyme does not recover completely after processing, for example, by cooling after steaming and pelletizing. Irreversible inactivation and reduced activity of an enzyme is generally not desirable in processes such as vapor spraying.

[005] When compared to dry feed mixtures, the feed pellets have properties that are favored by the industry, such as improved food quality, reduction of pathogens, lower levels of dust during manufacture, ease of handling, and more uniform dosage of ingredients . The preferred pelletizing processes in the industry use steam injection, in a process known as conditioning, which adds moisture and raises the temperature before the pelletizing step, which forces the feed ingredients heated by steam, or conditioned paste, through a matrix. The temperatures in the pelletizing process can be about 70 ° C to 95 ° C, or higher.

[006] Due to the steam, temperatures, compression forces and chemicals used in pelletizing processes, the activity or potency of the enzymes is often significantly reduced during processing and subsequent storage. In fact, feed enzymes are often supplied to the industry as stabilized liquid products that are sprayed onto the feed pellets after the pelletizing process, in order to avoid inactivating the enzyme. However, homogeneous dosing is difficult to achieve when the enzyme is applied after pelletizing, for example, by spraying the enzyme on pellets, and the cost of equipment to add the enzyme after pelletizing is high. Alternatively, liquid enzyme formulations, or dry mix enzyme formulations, can be added to the mixer prior to pelleting. In certain cases, higher levels of enzymes than otherwise necessary can be added in order to compensate for losses during pelletizing.

[007] There is a need in the detergent, textile, bakery, food and feed industry for stable, durable enzyme granules to serve as components in premix formulations and products, which are stored for several months or years, or subjected to industrial processing operations, such as mixing or pelletizing processes by steam treatment, without appreciable loss of enzymatic activity.

[008] Approaches to avoid the problem of irreversibly inactivating enzymes or reducing enzyme activity in industrial processes include identifying new sources of an enzyme (for example, identifying a known enzyme in an extreme thermophilic microorganism) or identifying ways to stabilize known enzymes. Klibanov, 1983, (Stabilization of Enzymes against Thermal Inactivation, Advances in Applied Microbiology, volume 29, page 1-28) reveals that there are three basic ways to stabilize enzymes: (1) immobilization, (2) chemical modification, (3) inclusion additives. However, Kli- banov (1983) also reveals that any of these methods could lead to stabilization or destabilization, or have no effect. Although previous approaches to formulation have made some progress in this area (see for example, WO97 / 23606, WO9854980, WO9739116, WO2007044968, EP1224273B1, WO2009 / 102770, US Patent 6,602,841, EP1804592B1, EP0098631B1 and EP1996028) in the present teachings further progress in overcoming some of these problems by using an improved granule structure.

[009] It is often desirable to provide a solid formulation with a high concentration of active enzyme in a granular or particulate form. The particulate granule is formulated with coatings, barrier coatings and additives or excipients designed to provide protection from the enzyme against inactivation. To minimize the cost of these protective coatings, additives and excipients, and to minimize the impact of product shipping costs, it is desirable to formulate the granular enzyme with the highest viable ratio of active enzyme to inactive ingredients. However, if the particle size of the granules is kept constant as the enzyme concentration, or payload is increased, the number of particles for a given amount of enzyme dosed in the final product or application decreases. A very large reduction in the number of particles per dose, however, can lead to high variability between individual doses of the product. For example, a low average number of enzyme granules in a detergent scoop, textile treatment mix, bread dough, or animal feed portion, can lead to an inhomogeneous distribution of enzyme concentration between individual doses or portions, and, therefore, the variability in the effectiveness of the enzyme treatment from dose to dose.

[0010] To achieve the economic benefits of the increased enzyme payload, to the extent that problems of variability resulting from a shortage of particles per dose are avoided, one can try to reduce the average particle diameter of the enzyme granule to maintain a sufficient number of particles per dose. However, it is difficult to apply enzyme coatings and protective barrier coatings to smaller core particles in coating processes, such as fluidized bed spray coating, drum coating, tipping coating and the like. As the particle diameter of the nuclei becomes smaller, particularly less than about 250, less than 200, less than 175, less than 150 microns, the tendency of the particles to clump increases. In addition, applying a weight percentage of coating material to a smaller core results in a thinner coating, therefore, a less protective coating. The problem of applying uniform coatings to small cores is challenging, particularly when the particles are irregularly shaped. Applying a coating to cores with sharp or angular corners and edges on the surface can result in regions of thin or discontinuous coating. Coatings applied to irregular coatings may also not adhere, or may result in agglomerates where roughness or superficial protuberances of neighboring core particles come into contact with each other. The compensation for this through the application of thicker coatings, if it can be done without agglomeration, will tend to add cost.

[0011] Therefore, there is a need for improved methods of applying enzyme coatings to cores without incurring significant agglomeration, while ensuring effective and continuous protective coatings. Achieving such high quality coatings in smaller particles will provide optimized stability of the enzymes during processing and storage, while providing greater payloads, and maintaining adequate homogeneity of the mixture so as not to increase dose-to-dose variability. dose.

BRIEF SUMMARY OF THE INVENTION

[0012] An objective of the invention is to ensure or improve the stability of an enzyme during storage only, industrial processing, or in mixtures with other ingredients, while at the same time, improving the ability to apply a continuous coating of consistent minimum thickness to the particle, that is, a coating with a reduced number of defects or thinning points in relation to the weight percentage of the coating material applied to the core or core containing the enzyme.

[0013] It has been surprisingly found that the supply of cores that have a smoother, more regular surface and a narrower particle size distribution allows for the application of improved coatings, with minimum thickness consistent with smaller particles. In some embodiments, enhanced coated enzyme granules are achieved, starting from a population of cores with a smoothness index less than 2.5, an average particle diameter, of average weight, less than 300 microns, and an index of particle size dispersibilities (PSDI) less than 2.0, and coating the enzyme-containing cores with an additional coating comprising a polymer comprising at least 7% w / w of the final granule.

BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 represents illustrative small smooth-core particles according to the present teachings, compared to other core particles that are small, but irregular.

[0015] Figure 2 represents some illustrative data according to the present teachings.

[0016] Figure 3 represents some illustrative data according to the present teachings.

[0017] Figure 4 is a graph that represents the pelletizing results of three batches of representative granules. The black bars represent the activities of the paste, while the dark gray and light gray bars represent the recovery yields at 90 ° C and 95 ° C, respectively.

[0018] Figure 5 is a graph representing the pelletizing results of three batches of representative phytase standard TPT. The black bars represent the activities of the paste, while the dark gray and light gray bars represent the recovery yields at 90 ° C and 95 ° C, respectively.

DETAILED DESCRIPTION

[0019] The practice of the present teachings will employ, unless otherwise indicated, conventional techniques of molecular biology (including recombination techniques), microbiology, cell biology, biochemistry, granulation and pelletizing of animal feed, which are within the scope of practice of the technique. Such techniques are fully explained in the literature, for example, Molecular Cloning: A Laboratory Manual, second edition (Sambrook et al., 1989); Oligonucleotide Synthesis (MJ Gait, ed., 1984; Current Protocols in Molecular Biology (FM Ausubel et al., Eds., 1994); PCR: The Polymerase Chain Reaction (Mullis et al., Eds., 1994); Gene Transfer and Expression: A Laboratory Manual (Kriegler, 1990), Granulation Technology for Bioproducts (Kadam, 1990), and Fairfield, D. 1994. Chapter 10. Pelleting Cost Center. In Feed Manufacturing Technology IV. (McEllhiney, editor), American Feed Industry Association, Arlington, Va., Pp. 110-139.

[0020] Unless otherwise defined, all the technical and scientific terms used in this document have the same meaning as normally understood by those versed in the technique to which the present teachings belong. Singleton, et al., Dictionary of Microbiology and Molecular Biology, second ed., John Wiley and Sons, New York (1994), and Hale & Markham, The Harper Collins Dictionary of Biology, Harper Perennial, NY (1991) provides the verse in the art a general dictionary with many of the terms used in the present invention. Any methods and materials similar or equivalent to those described in this document can be used in the practice or testing of the present teachings.

[0021] For ease of reference, the elements of the present teachings described under one or more titles. It should be noted that the teachings under each title also apply to teaching under the other titles. For example, each of the reported modalities and aspects related to the use of the present teachings is also a modality or aspect related to the method of the present teachings or the composition of the present teachings. Similarly, each of the reported modalities and aspects related to the use method of the present teachings is also a modality or aspect related to the composition of the present teachings. In the present patent application, the use of the singular includes plural, unless otherwise specifically reported.

[0022] All patents, patent applications, publications, documents, and articles cited in this document are hereby incorporated, in their entirety, by reference.

[0023] Numeric ranges provided in this document are inclusive of the numbers defining the range.

DEFINITIONS

[0024] As used here, the term "granule" refers to a particle that contains a nucleus (typically, a small smooth-core particle), an active agent (typically, an enzyme), and, optionally, at least one additional coating.

[0025] As used here, the term "core" refers to the inner core of a granule, and typically comprises a "small smooth-core particle". The cores of the present teachings can be produced by a variety of manufacturing techniques including: rotary atomization processes, wet granulation, dry granulation, spray drying, disk granulation, extrusion, drum coating, spheronization, tipping granulation, agglomeration fluidized bed, high shear granulation, fluidized bed spray coating, crystallization, precipitation, emulsion gelling, spinning disk atomization, and other fusion molding approaches, peptization processes. Such processes are known in the art and are described in US Patent No. 4689297 and in US Patent No. 5324649 (fluidized bed processing); EP656058B1 and US Patent No. 454332 (extrusion process): US Patent No. 6248706 (granulation, high shear); and EP804532B1 and US Patent No. 6534466 (combined processes using a fluidized bed core and mixer coating.

[0026] The active agent is typically coated around the nucleus. Cores suitable for use in the present teachings can be any material given the smoothness index, median mass diameter, and particle size disparity definitions, as provided herein. In one embodiment of the present teachings, the core comprises a crystal of sodium chloride or sodium sulfate. In another embodiment of the present teachings, the nucleus comprises a crystal seed of sucrose. Particles composed of inorganic salts and / or sugars and / or small organic molecules can be used as the nuclei of the present teachings. Water-soluble ingredients suitable for incorporation into the cores include: inorganic salts, such as sodium chloride, ammonium sulphate, sodium sulphate, magnesium sulphate, zinc sulphate; or urea, citric acid, sugars, such as sucrose, lactose and the like. In some embodiments, the core particles are crystalline inorganic salts. In some embodiments, the core particles are crystalline sodium sulfate.

[0027] The term "small smooth-core particle" as used here, refers to a particle, typically the inner particle of a granule, which exhibits a certain smoothness index, median mass diameter, and measures of dispersibility index particle size, as provided herein.

[0028] The term "smoothness index" as used here, refers to the ratio of the specific empirical surface area to the specific surface area of the envelope, calculated for a representative sample of cores.

[0029] As used here, "representative sample of nuclei" refers to a random sampling of at least 15 particles from the nucleus.

[0030] The term "empirical specific surface area" as used here, refers to the empirically measured surface area per gram (measured in square meters per gram) of a representative core sample as determined, using the method of adsorption of BET gas (Brunauer-Emmett-Teller) (S. Brunauer, PH Emmett and E. Teller J. Am. Chem. Soc., 1938, 60, 309. doi: 10.1021 / ja01269a023). The specific details of the BET method are described in the Methods section below.

[0031] The term "envelope specific surface area" as used here, refers to the calculated specific surface area (in units of square meters per gram) based on the measured particle size distribution and average true density of a representative sampling of nuclei, using the idealized assumption that all particles in the nucleus are perfect spheres. An algorithm for calculating the specific surface area of the envelope is described in the Methods section below.

[0032] The term "particle size distribution" (abbreviated DTP) as used here, refers to the relative amount in percentage by mass of small smooth core particles present in each of the various diameter size ranges. The DTP of the core sample is measured by laser light scattering (see for example T. Allen, Particle Size Measurement, Vol. 1 (Chapman and Hall, 1997). In the present application, DTP is characterized by three percentile diameters in bulk, which can be determined from a DTP log-normal plot.

[0033] D50 or MMD (mass median diameter) The log-normal distribution of the median mass diameter. MMD is considered to be the mass average particle diameter, below which 50% w / w of the particles of a representative sample have a smaller diameter.

[0034] D10: Tenth percentile in diameter (TPD), the diameter in which 10% w / w of the particles in a representative sample have a smaller diameter.

[0035] D90: Ninetieth percentile in diameter (NPD), the diameter in which 90% w / w of the particles in a representative sample have a smaller diameter.

[0036] Typically, D10, D50 and D90 are expressed in microns.

[0037] The "particle size dispersibility index" (abbreviated PSDI) of a core sample is the D90 / D10 ratio of a representative sample.

[0038] The term "coating" as used here, refers to a layer of material around an underlying material. The first coating layer generally encapsulates the core to form a substantially continuous layer so that the surface of the core has little or no uncoated area, and is typically an active agent coating. Subsequent additional coating layers can encapsulate the growing granule to form one or more additional substantially continuous layers ("additional coatings"). The materials (for example, the active agents and components detailed in this document) used in the granule may, but need not be, suitable for use in food and / or animal feed, and, consequently, may be food grade or feed grade.

[0039] The coatings of the present teachings may also include one or more of the following: inorganic salts, salts of organic acids, sugars, sugar alcohols, starches (native, pregelatinized, hydrolyzed or chemically modified) and other polysaccharides saccharides, gums, additional active agents, food-grade or feed-grade polymers, pigments, clays, plasticizers, surfactants, fibrous materials, anti-adhesion agents, fillers, extenders and other compounds known to be used in coatings. Suitable polymers include poly (vinyl alcohol) (PVA), including fully and partially hydrolyzed PVA, polyethylene glycol, polyethylene oxide, polyvinyl pyrrolidine, and carbohydrate polymers (such as, starch, amylose, amylopectin, alpha and beta-glucans, pectin, glycogen), including mixtures and their derivatives. Suitable fillers useful in coatings include inert materials used to add volume and reduce cost, or used for the purpose of adjusting the desired enzyme activity in the finished granule. Examples of such fillers include, but are not limited to, water-soluble agents, such as salts, sugars and water-dispersible agents, such as clays, talc, silicates, cellulose and starches, and cellulose and starch derivatives. Suitable plasticizers useful in the coatings of the present teachings are low molecular weight organic compounds and are highly specific for the polymer to be plasticized. Examples include, but are not limited to, sugars (such as, glucose, fructose and sucrose), sugar alcohols (such as sorbitol, xylitol, maltitol and other glycols), low molecular weight organic compounds, such as urea, or other plasticizers known as water or feed grade plasticizers. Suitable fibrous materials useful in the coatings of the present teachings include, but are not limited to: cellulose and cellulose derivatives, such as HPMC (hydroxy-propyl-methyl cellulose), CMC (carboxymethylcellulose), HEC (hydroxy-ethyl cellulose). In one embodiment, particularly for feed applications, of the present teachings, a coating comprises a corn cob material dispersible or soluble in water or sugar or crystalline salt. In another embodiment, particularly suitable for domestic cleaning applications, the coating comprises a dispersible or water-soluble sugar or crystalline salt or sugar spheres (non pareil). Those skilled in the art will recognize that, for applications in animal feed and food, coatings (and any polymers, fillers, plasticizers, fibrous and extender materials) are acceptable for applications in animal feed and / or food. For home cleaning applications, such a restriction need not be applied.

[0040] The term "active agent coating" as used here, refers to the coating containing the active agent (typically, an enzyme). In general, the active agent coating will be applied to the small smooth core particle, and additional coatings can optionally be applied later.

[0041] The term "additional coating" as used here, refers to one or more coatings that are optionally applied to a small smooth core particle. Typically, the additional coating will be applied to a nascent granule that contains a small smooth core particle and active agent coating. Non-limiting examples of additional coatings include moisture barrier coatings and moisturizing moisturizing coatings.

[0042] The term "moisture barrier coating" as used here, refers to a coating that comprises a moisture barrier material.

[0043] The term "moisturizing moisturizing coating" as used herein, refers to a coating comprising a moisturizing moisturizing material.

[0044] The term "moisture barrier coating" refers to materials that substantially exclude, prevent or delay the absorption of water. These materials are typically hydrophobic or amphiphilic, provide insulation against water and, inherently, do not absorb and / or bind water, and include, but are not limited to, film forming materials. Examples of moisture barrier materials include barrier polymers, proteins, lipids, fats and oils, fatty acids and gums. Examples of film-forming moisture barrier materials are natural or modified barrier polymers, such as gum arabic, whey, whey protein concentrate, PVA, including modified PVA and fully hydrolyzed PVA, and synthetic polymers, such as such as latex, HPMC, and hydroxy propyl acid-fluidized starch, for example, PureCote®, oxidized starch and modified starch. Non-film forming moisture barrier materials include, for example, waxes, fats, oils, lipids and lecithin. Selected moisture barrier materials that do not readily oxidize are, for example, latex polymer and barrier polymers such as gum arabic.

[0045] The term "moisturizing moisturizing material" refers to materials that absorb aqueous liquids, such as water, through a variety of mechanisms. In a first non-limiting mechanism, the materials absorb free water. In a second non-limiting mechanism, the materials absorb bound water, which is usually present as crystalline waters of hydration. Consequently, the materials can be supplied as partially or fully hydrated materials or as non-hydrated materials that will absorb or bind aqueous liquids, and will slow or reduce the rate or extent of migration of such liquids to the active agent. In a third non-limiting mechanism, moisturizing moisturizing materials thermally isolate the active agent by delaying the heat transfer to the active agent within the granule, and by keeping the active agent at a temperature lower than the temperature on the outer surface of the granule. . Humidifying moisturizing materials include carbohydrates and inorganic salts, including hydrated salts such as magnesium sulfate, sodium sulfate, and ammonium sulfate; maltodextrin; sugars, for example, sucrose; starch, including corn starch.

[0046] As used herein, the terms "pellets" and "pelletizing" refer to solid, rounded, spherical and cylindrical tablets or pellets, and to the processes of forming such solid forms, particularly feed pellets and animal feed solid extruded. Known animal feed and pellet food manufacturing processes generally include mixing the food or animal feed ingredients for about 1 to about 5 minutes at room temperature, transferring the resulting mixture to a silo, transporting the mixture to a steam conditioner, optionally transfer the steam-conditioned mixture to an expander, transfer the mixture to the pelletizing mill or extruder, and finally transfer the pellets to a pellet cooler. Fairfield, D. 1994. Chapter 10. Pelleting Cost Center. In Feed Manufacturing Technology IV. (McEllhiney, editor), American Feed Industry Association, Arlington, Va., Pp. 110-139.

[0047] As used herein, the term "non-pelleted mixtures" refers to premixes or precursors, base mixes, paste, and diluents. Premixes typically contain vitamins and micronutrients. Base mixtures typically contain food or feed ingredients, such as dicalcium phosphate, limestone, salt and premix of vitamins and minerals, but not grains and protein ingredients. Diluents include, but are not limited to, grains (eg wheat bran and rice bran) and clays, such as phyllosilicates (magnesium silicate sepiolite, bentonite, kaolin, montmorillonite, hectorite, saponite, beidelite, ata- pulgita and stevensita). Clays also function as vehicles and fluidizing agent, or diluents, for feed premixtures. The paste typically comprises a complete animal diet.

[0048] As used here, the term "recovered activity" refers to the ratio between (i) the activity of an active agent after a treatment involving one or more of the following stressors: heating, pressure increase, pH increase, decreased pH, storage, drying, exposure to surfactant (s), exposure to solvent (s) (including water / moisture), and mechanical stress) and (ii) phytase activity before treatment. The recovered activity can be expressed as a percentage.

[0049] The percentage of recovered activity is calculated as follows:

[0050] In the context of pelletizing experiments, "activity before treatment" can be approximated by measuring the activity of the active agent present in the paste that has not been subjected to treatment, in a way that is otherwise correlated to active agent that has undergone treatment.

[0051] For example, the active agent in the untreated paste is handled and stored for a similar time and under similar conditions, as the active agent in the treated paste (for example, pelletized), to control possible interactions or other unrelated effects. specified treatment itself.

[0052] As used here, the term "active agent" can be any material that must be added to a granule to provide the functionality intended for a particular use. The active agent can be a biologically viable material, a feed or food ingredient, an antimicrobial agent, an antibiotic substituting agent, a prebiotic, a probiotic, an agrochemical ingredient, such as a pesticide, fertilizer or herbicide; a pharmaceutical ingredient or an active ingredient for home care, or combinations thereof. In a preferred embodiment, the active ingredient is a protein, enzyme, peptide, polypeptide, amino acid, carbohydrate, lipid or oil, vitamin, co-vitamin, hormone, or combinations thereof. In another embodiment, the active ingredient is an enzyme, bleach, bleach activator, perfume, or another biologically active ingredient. Inherently thermostable active agents are covered by the present teachings and may exhibit improved thermostability in the granules. The most preferred active ingredients for food and feed applications are enzymes, peptides and polypeptides, amino acids, antimicrobial agents, agents that promote intestinal health, vitamins, and combinations thereof. Any enzyme can be used, and a non-limiting list of enzymes includes phytases, xylanases, β-glucanases, phosphatases, proteases, amylases (alpha or beta or glycoamylases), cellulases, lipases, cutinases, oxidases, transferases, reductases, hemicellulases , mannanases, esterases, isomerases, pectinases, lactases, peroxidases, laccases, other redox enzymes, and mixtures thereof. Particularly preferred enzymes include a Trichoderma reesei xylanase and a Trichoderma reesei xylanase variant, both available from DuPont Industrial Biosciences or the inherently thermostable xylanase described in EP1222256B1, as well as other Aspergillus niger xylanases, Aspergillus kisilligis and kispergillus kis , Aspergillus clavatus, Bacillus circulans, Bacillus pumilus, Bacillus subtilis, Fusarium verticilloides, Fusarium oxysporum, Neocallimastix patriciarum, Penicillium species, Streptomyces lividans, Streptomyces thermoviolaceus, Thermomonospora fusca, Tri- choderma, Trichoderma, Trichoderma, Trichoderma. Particularly preferred additional enzymes include phytases, such as Finase L®, a phytase of Aspergillus sp., Available from AB Enzymes, Darmstadt, Germany; Phyzyme® XP, an E. Coli phytase and Axtra Phy, a Buttiauxella phytase, both available from Danisco Animal Nutrition, DuPont and other phytases, for example, from the following organisms: Trichoderma, Penicillium, Fusarium, Buttiauxella, Citrobacter , Enterobacter, Penicillium, Humicola, Hafnia, Bacillus, and Peniophora, as well as those phytases described in US patent applications 61 / 595,923 and 61 / 595,941, both filed on February 12, 2012. An example of a cellulase is Multifect® BGL , a cellulase (beta glucanase), available from Danisco Animal Nutrition, DuPont, and other cellulases of species such as Aspergillus, Trichoderma, Penicillium, Humicola, Bacillus, Cellulomonas, Penicillium, Thermomonospore, Clostridium and Hypocrea. The cellulases and endoglucanases described in US20060193897A1 can also be used. The amylases can be, for example, from species such as Aspergillus, Tri-choderma, Penicillium, Bacillus, for example, B. subtilis, B. stearothermophilus, B. lentus, B. licheniformis, B. coagulans and B. amyloli- quefaciens. Suitable fungal amylases are derived from Aspergillus, such as A. oryzae and A. niger. Proteases can be from Bacillus amyloliquefaciens, Bacillus lentus, Bacillus subtilis, Bacillus licheniforms, and species of Aspergillus and Trichoderma. Phytases, xylanases, phosphatases, proteases, amylases, esterases, redox enzymes, lipases, transferases, cellulases and β-glucanases are enzymes frequently used for inclusion in animal feed. Enzymes suitable for inclusion in tablets for home care applications are similar, particularly, proteases, amylases, lipases, pectate lyases, mannanases, hemicellulases, redox enzymes, peroxidases, transferases and cellulases. In particularly preferred aspects of the present teachings, enzymes are selected from phytases, xylanases, beta-glucanases, amylases, proteases, lipases, esterases, and mixtures thereof. In one embodiment of the present invention, two enzymes are supplied in the granule, a xylanase and a beta-glucanase. In another embodiment of the present invention, two enzymes are supplied in the granule, they are a protease and an amylase. Enzymes can be mixed together or applied to the granule separately. In another embodiment, three enzymes are supplied in the granule, namely, beta-glucanase, xylanase and phytase. The enzyme lists above are examples only, and should not be exclusive. Any enzyme can be used in the granules of the present teachings, including wild type enzymes, recombinants and variants from bacterial, fungal, yeast, vegetable, insect and animal sources, and acidic, neutral or alkaline enzymes. It will be recognized by those skilled in the art that the amount of enzyme used will depend, at least in part, on the type and property of the selected enzyme and the intended use. METHODS OF SPECIFIC BET SURFACE MEASUREMENT.

[0053] To measure the specific surface area of the nucleus particles, the BET method was used to evaluate the pore distribution of the nuclei by krypton gas adsorption / desorption, using a Micromeritics Accelerated Porosimetry and Surface Area System model ASAP 2420 The BJH method (Elliott P. Barrett, Leslie G. Joyner, Paul P. Halenda, J. Am. Chem. Soc., 1951, 73 (1), pp 373-380, DOI: 10.1021 / ja01145a126) was used to determine the pore volume distribution in the range of 20 - 1000 Â. Samples, approximately 4-6 grams by mass, were degassed under vacuum at 150 ° C overnight to remove adsorbed water and / or volatile contaminants. They were re-weighed and transferred to the porosimeter, placed under vacuum and cooled to -196 ° C (77K), at which point the gas adsorption isotherms (Kr) were recorded using pressure measurements. Coverage of the surface area is automatically calculated by the ASAP 2420 V2.07J software package, available from Micromeritics. CALCULATION OF THE SPECIFIC SURFACE AREA OF THE WRAP.

[0054] The specific surface area of the envelope represents the specific surface area of an idealized equivalent sample of spherical, perfectly smooth particles whose DTP is correlated with that of the actual core sample. The particle size distribution (DTP) of the cores is determined by laser diffraction (reference ISO 13320-1: 1999) using a Malvern Mastersizer 2000 with hexane as the background fluid in the recirculation loop. A quantity of the core sample is added with sufficient mass to achieve a laser obscuration of approximately 10%. DTP is automatically calculated from the diffraction pattern, using Mie's theory. In the case of sodium sulfate, the complex refractive index of the particle is taken as 1.468 + 0.01i, and the fluid refractive index as 1.375. The DTP generated is a fraction of the differential mass showing the percentage of particles in each of the 81 intervals (bins) of logarithmically spaced sizes ranging from 0.01 micron to 10,000 microns. If necessary, DTP should be corrected and renormalized to exclude artifacts such as fine dust particles or agglomerates, specifically, any particle finer than 60 microns or greater than 500 microns.

[0055] The geometric mean diameter of the upper and lower size demarcations of each size range is calculated by taking the square root of the product of the upper and lower size demarcation. The specific surface area of the envelope in square meters per gram for a given interval is given by 6 / (d * p), where d is the particle diameter (in cm), p is the true density (density excluding empty spaces) of solid particulates (in g / cm3). In the case of sodium sulfate, p = 2.664 g / cm3. Since DTP is a weighted mass distribution, the total surface area of the envelope is calculated by multiplying the fraction of differential mass in each size range by the specific surface area for that size range, and adding up all the ranges of size.

EXEMPLIFYING MODALITIES

[0056] In some embodiments, the present teachings provide a granule composition that surprisingly has equivalent stability compared to granules containing smooth cores that are not small, thus allowing material cost savings. For example, the granule composition of the present teachings can have surprisingly equivalent storage stability. Desirable storage stability can reside in any of several contexts, including dishwashing detergent, laundry detergent, animal feed, and human food. The granules of the present teachings can demonstrate better stability in these various conditions compared to granules stored in an identical manner without the small smooth core particle of the present teachings. In some embodiments, the present teachings are believed to provide improved stability compared to granules containing larger and / or non-smooth cores.

[0057] In some embodiments, the present teachings provide a granule composition with equivalent resistance to dust generation compared to granules that do not contain small smooth cores as measured by the Heubach dust test.

[0058] In some embodiments, the present teachings are believed to provide a granule composition with improved resistance to dust generation compared to granules that do not contain a small smooth core.

[0059] In some embodiments, the present teachings provide a granule composition of resistance equivalent to steam plating, compared to granules that do not contain small smooth cores. For example, the degree of "recovered activity", as defined above, can be determined for the granules of the present teachings, and compared to that of the granules treated in an identical manner, containing a different nucleus from the small smooth core particle of the present teachings. In some embodiments, the present teachings are believed to provide a granule composition of improved resistance to vapor pelletization compared to granules containing a small smooth core.

[0060] Additional applications and methods using the granules of the present teachings are described in the non-limiting sections below. COMPOSITIONS AND METHODS FOR BAKING AND PREPARING FOOD

[0061] The present invention also relates to a "food composition", including but not limited to a food product, animal feed and / or food / feed additives, comprising the present granule composition, and methods for the preparation of such a food composition comprising mixing the present granule composition with one or more food ingredients, or the uses thereof.

[0062] The present granule composition can be used in the preparation of a food composition, wherein the food composition is baked subsequent to the addition of the present granule composition. As used herein, the term "baking composition" means any composition and / or additive prepared in the process of providing a baked food product, including but not limited to baker's flour, bread dough, baking additive and / or a baked product. The composition of the food or additive can be liquid or solid.

[0063] As used here, the term "flour" means ground or ground cereal grain. The term "flour" can also mean sago or tuber products that have been ground or kneaded. In some embodiments, flour may also contain components in addition to vegetable material or crushed or crushed cereal. An example of an additional component, although not intended to be a limiting factor, is a fermenting agent. Cereal grains include wheat, oats, rye and barley. Tuber products include tapioca flour, cassava flour and powdered cream. The term "flour" also includes ground corn flour, corn bran, rice flour, wholegrain flour, leavened flour, tapioca flour, cassava flour, ground rice, enriched flower and cream powder.

[0064] For the commercial and domestic use of flour for baking and food production, it is important to maintain an adequate level of enzymatic activity in the flour. A level of activity that is too high can result in a product that is sticky and / or pasty and therefore not marketable. Flour with insufficient enzyme activity may not contain enough sugar for proper yeast function, resulting in baked products or dry, brittle breads. Consequently, the present granule composition in combination with α-amylase (s), can be added to the flour to increase the level of endogenous enzyme activity in the flour.

[0065] An amylase in the present granule composition can be added alone or in combination with other amylases to prevent or delay aging, that is, hardening of the core of baked products. The amount of anti-aging amylase will typically be in the range of 0.01-10 mg of enzyme protein per kg of flour, for example, 0.5 mg / kg G.S. Additional anti-aging amylases that can be used and include an en-amylase, for example, a bacterial endo-amylase from Bacillus. The additional amylase can be another maltogenic α-amylase (EC 3.2.1.133), for example, Bacillus. Novamyl® is a maltogenic α-amylase that exemplifies B. stearothermophilus from the NCIB 11837 strain, and is described, for example, in Christophersen et al. (1997) Starch 50: 39-45. Other examples of anti-aging endo-amylases include bacterial α-amylases, derived from Bacillus, such as B. licheniformis or B. amyloliquefaciens. Anti-aging amylase can be an exo-amylase, such as β-amylase, for example, from vegetable sources, such as soy, or from microbial sources, such as Bacillus.

[0066] The baking composition comprising the present granule composition can further comprise a phospholipase or enzyme with phospholipase activity. An enzyme with phospholipase activity has an activity that can be measured in units of Lipase (LU). Phospholipase can have A1 or A2 activity to remove fatty acids from phospholipids, forming a lysophospholipid. It may or may not have lipase activity, that is, activity on triglyceride substrates. Phospholipase typically has an optimal temperature in the range of 30-90 ° C, for example, 30-70 ° C. The phospholipases added may be of animal origin, for example, from the pancreas, for example, bovine or porcine pancreas, snake venom, or bee venom. Alternatively, the phospholipase can be of microbial origin, for example, of filamentous fungi, yeasts or bacteria, for example.

[0067] Phospholipase is added in an amount that improves the bread's softness during the initial period after baking, particularly in the first 24 hours. The amount of phospholipase will typically be in the range of 0.01-10 mg of enzyme protein per kg of flour, for example, 0.1-5 mg / kg. That is, the phospholipase activity will generally be in the range of 20-1000 LU / kg of flour, where a Unit of Lipase is defined as the amount of enzyme needed to release 1 μmol of butyric acid per minute at 30 ° C, pH 7 , 0, with gum arabic as an emulsifier and tributyrin as a substrate.

[0068] Pasta compositions generally comprise wheat bran or wheat flour and / or other types of bran, flour or starch such as corn flour, corn starch, rye bran, rye flour, oat flour, oat bran , soy flour, sorghum bran, sorghum flour, potato bran, potato flour or potato starch. The dough can be fresh, frozen or partially baked. The dough can be a leavened dough or a dough to be subjected to fermentation. The dough can be fermented in several ways, such as by adding chemical fermenting agents, for example, sodium bicarbonate, or by adding a yeast, or, by fermenting the dough. The dough can also be fermented by adding a suitable yeast culture, such as a culture of Saccharomycescerevisiae (baker's yeast), for example, a commercially available strain of S. cerevisiae.

[0069] The dough may also comprise other conventional dough ingredients, for example, proteins, such as powdered milk, gluten and soy; eggs (for example, whole eggs, egg yolks or egg whites); an oxidizer, such as ascorbic acid, potassium bromate, potassium iodate, azodicarbonamide (ADA) or ammonium persulfate; an amino acid, such as L-cysteine; a sugar; or a salt, such as sodium chloride, calcium acetate, sodium sulfate or calcium sulfate. The dough may further comprise fat, for example, triglycerides, such as granulated fat or solid fat. The dough may further comprise an emulsifier such as mono or diglycerides, diacetyl esters of mono or diglycerides tartaric acid, fatty acid esters sugar, fatty acid polyglycerol esters, monoglyceride lactic acid esters, esters of monoglyceride acetic acid oils, polyoxyethylene stearates or lysolecithin. For example, the dough can be made without adding emulsifiers.

[0070] The dough product can be any processed dough product, including deep fried dips, deep-fried, baked, steamed and boiled, such as steamed rice cakes and bread. In one application, the food product is a bakery product. Typical bakery products (baked goods) include bread - such as breads, rolls, sweet rolls, donuts, pizza bases, etc., pasta, pretzels, tortillas, cakes, crackers, cookies, crackers, etc.

[0071] Optionally, an additional enzyme can be used together with anti-aging amylase and phospholipase. The additional enzyme can be a second amylase, such as an amyloglycosidase, a β-amylase, a cyclodextrin glucanotransferase, or the additional enzyme can be a peptidase, in particular, an exopeptidase, a transglutaminase, a lipase, a cellulase, a xylanase, a protease, a protein disulfide isomerase, for example, a protein disulfide isomerase as disclosed in WO 95/00636, for example, a glycosyltransferase, a branching enzyme (1,4-α-glucan branching enzyme), a 4-α-glucanotransferase (dextrin glycosyltransferase) or an oxidoreductase, for example, a peroxidase, a laccase, a glucose oxidase, a pyranose oxidase, a lipooxygenase, a L-amino acid oxidase or a carbohydrate oxidase. The additional enzymes can be of any origin, including mammals and plants and, particularly, of microbial origin (bacteria, yeasts or fungi), and can be obtained by techniques conventionally used in the art.

[0072] Xylanase is usually of microbial origin, for example, derived from a bacterium or fungus, such as a strain of Aspergillus. Xylanases include PENTOPAN® and NOVOZYM 384®, for example, which are commercially available preparations of xylinase produced from Trichoderma reesei. Amyloglycosidase can be an A. niger amyloglycosidase (such as AMG®). Other useful amylase products include Grindamyl® A 1000 or A 5000 (Grindsted Products, Denmark) and Amylase® H or Amylase® P (DSM). Glucose oxidase can be a fungal glucose oxidase, in particular, a glucose oxidase from Aspergillus niger (such as Gluzyme®). An example protease is Neutrase®.

[0073] The process can be used for any type of baked product prepared from pasta, with a soft or crunchy characteristic, of a white, light or dark type. Examples are bread, especially white, wholegrain bran or rye bread, typically in the form of breads or rolls, such as, but not limited to, French baguette bread, pita bread, tortillas, cakes, pancakes, cookies, crackers , pie crusts, toast, steamed bread, pizza and the like.

[0074] The present granule composition can be used in a premix, which comprises the flour together with an anti-aging amylase, a phospholipase and / or a phospholipid. The premix may contain other additives for improving the dough and / or improving the bread, for example, any of the additives, including enzymes, mentioned above. The present granule composition can be a component of an enzyme preparation, comprising an anti-aging amylase and a phospholipase, for use as a baking additive.

[0075] The storage, handling and incorporation of the loaded application vehicle can be carried out by means of a packaged mixture. The packaged mixture can comprise the present granule composition. However, the packaged mixture may still contain additional ingredients as required by the manufacturer or baker. After the present granule composition has been incorporated into the dough, the baker continues the normal production process for that product.

[0076] A food composition is contemplated where the food is a composition of oil, meat, lard, comprising the present granule composition. In this context, the term "[oil / meat / lard] composition" means any composition, based on, made from and / or containing oil, meat or lard, respectively. A method for preparing an oil, meat or lard and / or additive composition comprising the present granule composition is contemplated, which comprises mixing the present granule composition with an oil / meat / lard composition and / or ingredients additions.

[0077] The food composition may be an animal feed composition, animal feed additive, and / or pet food comprising the present granule composition. A method for preparing such an animal feed composition, animal feed additive composition and / or pet food is contemplated, which comprises mixing the present granule composition of the same with one or more feed ingredients for animals and / or animal feed additive ingredients and / or pet food ingredients. The present granule composition can be used in the preparation of an animal feed composition and / or animal feed additive composition, and / or pet food.

[0078] The term "animal" includes all non-ruminant and ruminant animals. In a given modality, the animal is a non-ruminant animal, like a horse and a mono-gastric animal. Examples of mono-gastric animals include, but are not limited to, pigs and pigs, such as piglets, growing pigs, sows; poultry such as turkeys, ducks, chicken, broilers, laying hens; fish such as salmon, trout, tilapia, catfish and carp; and crustaceans, like shrimp. In another embodiment the animal is a ruminant animal including, but not limited to, cattle, young calves, goats, sheep, giraffes, bison, elk, reindeer, yaks, buffalo, deer, camels, alpacas, llamas, antelope, antilocapras and nilgai.

[0079] In the present context, it is intended that the term "pet food" is understood as a food for a domestic animal such as, but not limited to, dogs, cats, gerbils, hamsters, chinchillas, luxury mice, piggies from India; pet birds, such as canaries, parakeets and parrots; reptile pets, such as turtles, lizards and snakes; and aquatic pets, such as tropical fish and frogs.

[0080] The terms "animal feed composition", "feed" and "livestock feed" are used interchangeably and may comprise one or more food materials selected from the group comprising a) cereals, such as small grains ( for example, wheat, barley, rye, oats and their combinations) and / or large grains such as corn or sorghum; b) for cereal products, such as corn gluten bran, distillers dry grain solubles (DDGS) (particularly corn based on distillers dry grain solubles (cDDGS), wheat bran, wheat by-product, short wheat , rice bran, rice husks, oat husks, palm seed and citrus pulp; c) protein obtained from sources such as soy, sunflower, peanuts, lupine, peas, broad bean, cotton, canola, fish meal, proteins dry plasma, meat and bone meal, potato protein, whey, copra, sesame; d) oils and fats obtained from plant and animal sources; e) minerals and vitamins. COMPOSITIONS FOR TEXTILE DEGOMING AND USE

[0081] Compositions and methods for treating fabrics (for example, for uncoiling textile products) using the present granule composition are also contemplated. Methods for treating fabrics are well known in the art (see, for example, US Patent No. 6,077,316). For example, the tactile feel and appearance of a fabric can be improved through a method which comprises bringing the fabric into contact with the present granule composition in a solution. The fabric can be treated with the solution under pressure.

[0082] The present granule composition can be applied during or after weaving a textile product, or during the degumming phase, or in one or more additional fabric processing steps. During the weaving of textile products, the yarns are exposed to considerable mechanical stress. Before weaving on mechanical looms, weft yarns are often coated with ironing starch or starch derivatives to increase their tensile strength and to prevent breakage. The present granule composition can be applied during or after weaving to remove these starch or starch derivatives. After weaving, an amylase can be used to remove the ironing lining prior to further processing of the fabric to ensure a homogeneous and wash-proof result.

[0083] The present granule composition can be used alone or with other chemical degreasing reagents and / or degreasing enzymes to degrease fabrics, including fabrics containing cotton, as detergent additives, for example, in aqueous compositions. The present granule composition can also be used in compositions and methods to produce a "washed with stone" (stoned) appearance on garments and denim-dyed denim fabrics. For the manufacture of clothes, the fabric can be cut and sewed on clothes or garments, which are then finished. In particular, for the manufacture of jeans, different enzymatic finishing methods have been developed. The finishing of the denim garment is usually started with an enzymatic degreasing step, during which the garments are subjected to the action of amylolytic enzymes to provide softness to the fabric and make cotton more accessible for the finishing steps subsequent enzymatic reactions. The present granule composition can be used in methods of finishing denim garments (for example, a "bio-stamping" process), enzymatic degumming and providing softness to fabrics, and / or finishing.

CLEANING COMPOSITIONS

[0084] One aspect of the present compositions and methods is a cleaning composition that includes the present granule composition as a component. An amylase polypeptide, or other enzyme of interest, can be used as a component in detergent compositions for hand washing, washing in a washing machine, washing dishes, and cleaning other hard surfaces.

[0085] Preferably, the present granule composition is incorporated in detergents at or near a concentration conventionally used for amylase in detergents. For example, a protease polypeptide can be added in an amount corresponding to 0.00001 - 1 mg (calculated as pure enzyme protein) of amylase per liter of dishwashing / washing liquor. Sample formulations are provided in this document, as exemplified by the following:

[0086] A protease polypeptide can be a component of a detergent composition, as the only enzyme or with other enzymes including other amylolytic enzymes. As such, it can be included in the detergent composition in the form of the present granule composition. As used in this context, examples of non-limiting waxy coating materials are products of poly (ethylene oxide) (polyethylene glycol, PEG) with average molar weights of 1,000 to 20,000; ethoxylated nonylphenols containing 16 to 50 ethylene oxide units; ethoxylated fatty alcohols in which the alcohol contains 12 to 20 carbon atoms and in which there are 15 to 80 ethylene oxide units; fatty alcohols; fatty acids; and mono- and di- and triglycerides of fatty acids. Examples of film-forming coating materials suitable for application by fluid bed techniques are given, for example, in Patent GB 1483591.

[0087] The detergent composition can comprise one or more surfactants, each of which can be anionic, non-ionic, cationic or zwitterionic. The detergent will generally contain 0% to about 50% of anionic surfactant, such as linear alcohol benzene sulfonate (LAS); α-olefinsulfonate (AOS); alkyl sulfate (fatty alcohol sulfate) (AS); ethoxysulfate alcohol (AEOS or AES); secondary alkane sulfonates (SAS); methyl esters of α-sulfo fatty acids; alkyl or alkenyl succinic acid; or soap. The composition may also contain 0% to about 40% of nonionic surfactant such as ethoxylated alcohol (AEO or AE), carboxylated alcohol ethoxylates, ethoxylated nonylphenol, alkyl polyglycoside, alkyldimethylamineoxide, ethoxylated fatty acid monoethanolamide, monoethanolamide or fatty acid monoethanolamide or fatty acid monoethanolamide or fatty acid monoethanolamide. of alkyl polyhydroxy fatty acid (as described for example in WO 92/06154).

The detergent composition may additionally comprise one or more other enzymes, such as another protease, another amylolytic enzyme, cutinase, lipase, cellulase, lyase pectate, perhydrolase, mannanase, xylanase, peroxidase and / or laccase in any combination.

[0089] The detergent may contain about 1% to about 65% of a detergent-reinforcing agent or complexing agent such as zeolite, diphosphate, triphosphate, phosphonate, citrate, nitrilotriacetic acid (NTA), ethylenediaminetetraacetic acid (EDTA), diethylene triami acid - nopentacetic (DTMPA), alkyl- or alkenyl-succinic acid, soluble silicates or layered silicates (eg SKS-6 by Hoechst). The detergent can also be not constructed, that is, essentially free of detergent reinforcing agent. Enzymes and specifically amylases, with or without binding starch domains, can be used in a variety of compositions including laundry and dishwashing applications, surface cleaners, as well as compositions for the production of starch or biomass ethanol. .

[0090] The detergent may comprise one or more polymers. Examples include carboxymethylcellulose (CMC), poly (vinylpyrrolidone) (PVP), polyethylene glycol (PEG), polyvinyl alcohol (PVA), polycarboxylates such as polyacrylates, maleic / acrylic acid copolymers and lauryl methacrylate / acrylic acid copolymers.

[0091] The detergent may contain a bleaching system, which may comprise a source of H2O2 such as perborate or percarbonate, which may be combined with a peracid-forming bleach activator such as tetraacetylethylenediamine (TAED) or nonanoaloxybenzenesulfonate (NOBS). Alternatively, the targeting system may comprise peroxyacids (for example, amide, imide or sulfone-type peroxyacids). The bleaching system can also be an enzymatic bleaching system, for example, perhydrolase, as described in International Patent Application WO 2005/056783.

The enzymes of the detergent composition can be stabilized using conventional stabilizing agents, for example, a polyol such as propylene glycol or glycerol; a sugar or sugar alcohol; lactic acid; boric acid or a boric acid derivative, such as, for example, an aromatic borate ester; and the composition can be formulated as described, for example, in WO 92/19709 and WO 92/19708.

[0093] The detergent may also contain other ingredients of conventional detergents, such as, for example, fabric conditioners, including clays, foam reinforcers, soap bubble eliminators, anti-corrosion agents, impurities suspending agents, anti-corrosion agents -reposition of impurities, dyes, bactericides, stain inhibitors, optical brighteners, or perfumes.

[0094] The pH (measured in aqueous solution, at the concentration of use) is generally neutral or alkaline, for example, pH from about 7.0 to about 11.0.

[0095] Particular forms of detergent compositions for inclusion in the present teachings are described below. DETERGENT COMPOSITION FOR WASHING DRY / SOLID HEAVY SERVICE CLOTHES (HDD)

[0096] Examples of HDD laundry detergent compositions include a detersive surfactant, including anionic detersive surfactants (e.g., straight or branched or random chain, substituted or unsubstituted alkyl sulfates, alkyl sulfonates, alkoxylated alkyl sulfate, alkyl phosphates, alkyl phosphonates, alkyl carboxylates and / or mixtures thereof, nonionic detersive surfactant (for example a straight or branched or random chain, substituted or unsubstituted C8-C18 ethoxylated alkyl, and / or C6C12 alkoxylated alkyl phenol), cationic detersive surfactant (alkyl pyridinium compounds, alkyl quaternary ammonium compounds, alkyl quaternary phosphonium compounds, ternary alkyl sulfonium compounds, and mixtures thereof); zwitterionic and / or amphoteric detersive surfactants (for example, sulpho-betaine alkanolamine); ampholyte surfactants; semi-polar nonionic surfactants and their mixtures; builders including phosphate-free builders (for example zeolite builders examples which include zeolite A, zeolite X, zeolite P and zeolite MAP in the range 0% by weight to less than 10% by weight), phosphate builders (for example from sodium tri-polyphosphate in the range of 0% by weight to less than 10% by weight), citric acid, citrate salts and nitrilotriacetic acid, silicate salt (for example, sodium or potassium silicate or sodium meta-silicate in the range from 0% by weight to less than 10% by weight, or layered silicate (SKS-6)); carbonate salt (for example, sodium carbonate and / or sodium bicarbonate in the range of 0% by weight to less than 80% by weight); and bleaching agents including photo-bleaches (for example, sulphonated zinc phthalocyanines, sulphonated aluminum phthalocyanines, xanthine dyes and mixtures thereof) hydrophobic or hydrophilic bleach activators (eg, dodecanoyloxybenzene sulfonate, decanoyl oxybenzene sulfate) , decanoyl oxybenzoic acid or salts thereof, 3,5,5-trimethyhexanoyloxybenzene sulfonate, tetraacetyl ethylene diamine-TAED, nano-oxybenzene sulfonate-NOBS, nitrile quats, and mixtures thereof, sources of hydrogen peroxide (for example, salts inorganic perhydrate examples of which include perborate sodium mono or tetrahydrate salt of perborate, percarbonate, persulfate, phosphate or persilicate), hydrophilic or hydrophobic preformed peracids (eg, percaboxylic acids and salts, percarbonic acids and salts, percarbonic acids, salts and perimidic salts, peroxymonosulfuric acids and salts and mixtures thereof), and / or bleach catalysts (for example, imine bleach boosters (eg examples of which include cations and iminium polyons), iminium zwitterions, modified amines, modified amine oxides, N-sulfonyl imines, N-phosphonyl imines, N-acyl imines, thiadiazole dioxides, perfluorimines, cyclic sugar ketones and their mixtures and metal containing bleach catalysts (for example, copper, iron, titanium, ruthenium, tungsten, molybdenum or manganese cations together with an auxiliary metal cation such as zinc or aluminum and a scavenger such as ethylenediaminetetraacetic acid, ethyl- nediaminetetramethylenophosphonic acid and its water-soluble salts).

The composition preferably includes enzymes, for example, proteases, amylases, lipases, cellulases, choline oxidases, peroxidases / oxidases, pectate lyases, mannases, cutinases, laccases, phospholipids, lysophospholipases, acyltransferase, perhydrolase, arylesterase and any mixture of these.

[0098] The composition may optionally include additional detergent ingredients including perfume microcapsules, encapsulated starch perfume, tinting agents, additional polymers, including fabric integrity and cationic polymers, dye fixing ingredients, fabric softening agents, brighteners (for example, fluorescent IC brighteners), flocculating agents, chelating agents, alkoxylated polyamines, tissue deposition aids, and / or cyclodextrin. DETERGENT COMPOSITION OF AUTOMATIC DISHWASHING (LAP)

[0099] The exemplary LAP detergent composition includes nonionic surfactants, including ethoxylated nonionic surfactants, alcohol alkoxylated surfactants, epoxy-terminated polyoxyalkyl alcohols or amine oxide surfactants present in quantities of 0 to 10%, by weight; reinforcing agents in the range of 5 to 60% including phosphate-reinforcing agents (eg mono-phosphates, di-phosphates, tri-polyphosphates, other oligomeric polyphosphates, sodium tripolyphosphate-STPP) and phosphate-free reinforcing agents (eg amino acid-based compounds including methyl-glycine-diacetic acid (MGDA) and salts and derivatives thereof, glutamic-N, N-diacetic acid (GLDA) and salts and derivatives thereof, iminodisuccinic acid (IDS) and salts and derivatives thereof, carboxy methyl inulin and salts and derivatives thereof, nitrilotriacetic acid (NTA), diethylene triamine penta acetic acid (DTPA), alaninediacetic acid-B (B-ADA) and its salts, homopolymers and copolymers of polycarboxylic acids and their partially salts or completely neutralized, monomeric polycarboxylic acids and hydroxycarboxylic acids and their salts in the range of 0.5% to 50% by weight; sulfonated / carboxylated polymers in the range of about 0.1% to about 50% by weight , to provide stability dimensional; drying aids in the range of about 0.1% to about 10% by weight (for example, polyesters, especially anionic polyesters, optionally together with other additional monomers with 3 to 6 functionalities - typically, acid functionalities, alcohol or ester that are favorable to polycondensation, polycarbonate, polyurethane and / or polyurea-polyorganosiloxane compounds or their precursor compounds, particularly of the cyclic reactive carbonate and urea type); silicates in the range of about 1% to about 20% by weight (including sodium or potassium silicates, for example, sodium disilicate, sodium meta-silicate and crystalline phyllosilicates); inorganic bleach (for example, perhydrate salts such as perborate, percarbonate, perphosphate, persulfate, and persilicate salts) and organic bleach (for example, organic peroxyacids, including diacyl and tetraacylperoxides, such as diperoxydecanedioic acid, diperoxytetrahydrate and diperoxytetrahydrate diperoxidohexadecanedióico acid); bleach activators (ie precursors of organic peracid, in the range of about 0.1% to about 10% by weight); bleach catalysts (for example, manganese triazacyclononane and related complexes, Co, Cu, Mn and Fe bispyridylamine and related complexes, and cobalt (III) acetate pentamine and related complexes); agents for treating metal in the range of about 0.1% to 5% by weight (for example, benzatriazoles, metallic and complex salts, and / or silicates); enzymes in the range of about 0.01 to 5.0 mg of active enzyme per gram of detergent composition for automatic dishwashing (eg proteases, amylases, lipases, cellulases, choline oxidases, peroxidases / oxidases, pectate lyases , mannanases, cutinases, laccases, phospholipids, lysophospholipases, acyltransferase, perhydrolase, arylesterase and their mixtures); and enzyme stabilizing components (eg, oligosaccharides, polysaccharides and inorganic salts of divalent metals). ADDITIONAL DETERGENT COMPOSITIONS

[00100] Additional examples of detergent formulations to which any of a variety of relevant enzymes in the present granule compositions can be added are described below, in the numbered paragraphs.

[00101] 1) a detergent composition formulated as a granulate, having a specific density of at least 600 g / L comprising linear alkylbenzenesulfonate (calculated as acid) from about 7% to about 12%; alcohol ethoxysulfate (eg C12-18 alcohol, 1-2 ethylene oxide (OE)) or alkyl sulfate (eg C16-18) about 1% to about 4%; alcohol ethoxylate (for example, C14-15, 7 EO alcohol) about 5% to about 9%; sodium carbonate (for example, Na2CO3) about 14% to about 20%; soluble silicate (for example, Na2O, 2SiO2) about 2 to about 6%; zeolite (for example, NaA1SiO4) about 15% to about 22%; sodium sulfate (for example, Na2SO4) 0% to about 6%; sodium citrate / citric acid (for example, C6H5Na3O7 / C6H8O7) about 0% to about 15%; sodium perborate (for example, NaBO3H2O) about 11% to about 18%; TAED about 2% to about 6%; carboxymethylcellulose (CMC) 0% to about 2%; polymers (for example, maleic / acrylic acid, copolymer, PVP, PEG) 0-3%; enzyme protein (calculated as pure enzyme) 0.0001-0.1%; and minority ingredients (for example, foam suppressants, perfumes, optical brighteners, foaming agents) 0-5%.

[00102] 2) A detergent composition formulated as a granulate having a specific density of at least 600 g / L comprising linear alkylbenzenesulfonate (calculated as acid) about 6% to about 11%; alcohol ethoxysulfate (for example, C12-18, 1-2 EO alcohol) or alkyl sulfate (for example, C16-18) about 1% to about 3%; ethoxylated alcohol (for example, C14-15, 7 EO alcohol) about 5% to about 9%; sodium carbonate (for example, Na2CO3) about 15% to about 21%; soluble silicate (for example, Na2O, 2SiO2) about 1% to about 4%; zeolite (for example, NaA1SiO4) about 24% to about 34%; sodium sulfate (for example, Na2SO4) about 4% to about 10%; sodium citrate / citric acid (for example, C6H5Na3O7 / C6H8O7) 0% to about 15%; carboxymethylcellulose (CMC) 0% to about 2%; polymers (for example, maleic / acrylic acid cup-polymer, PVP, PEG) 1-6%; enzymes (calculated as pure enzyme protein) 0.0001-0.1%; minority ingredients (eg foam suppressants, perfume) 0-5%.

[00103] 3) A detergent composition formulated as a granulate, having a specific density of at least 600 g / L comprising linear alkylbenzenesulfonate (calculated as acid) about 5% to about 9%; alcohol ethoxylate (for example, C12-15, 7 EO alcohol) about 7% to about 14%; soap such as fatty acid (for example, C16-22 fatty acid), about 1 to about 3%; sodium carbonate (like Na2CO3) about 10% to 17%; soluble silicate (for example, Na2O, 2SiO2) about 3% to about 9%; zeolite (like NaA1SiO4) about 23% to about 33%; sodium sulfate (for example, Na2SO4) 0% to 4%; sodium perborate (for example, Na-BO3H2O) about 8% to about 16%; TAED about 2% to about 8%; phosphonate (for example, EDTMPA) 0% to about 1%; carboxymethylcellulose (CMC) 0% to about 2%; polymers (for example, maleic / acrylic acid copolymer, PVP, PEG) 0-3%; enzymes (calculated as pure enzyme protein) 0.0001-0.1%; mi-noritar ingredients (eg foam suppressants, perfume, optical brightener) 0-5%.

[00104] 4) A detergent composition formulated as a granulate having a specific density of at least 600 g / L comprising linear alkylbenzenesulfonate (calculated as acid) about 8% to about 12%; ethoxylated alcohol (for example, C12-15, 7 EO alcohol) about 10% to about 25%; sodium carbonate (as Na2CO3) about 14% to 22%; soluble silicate (for example, Na2O, 2SiO2) about 1% to about 5%; zeolite (for example, NaA1SiO4) about 25% to about 35%; sodium sulfate (for example, Na2SO4) 0% to about 10%; carboxymethylcellulose (CMC) 0% to about 2%; polymers (for example, maleic / acrylic acid copolymer, PVP, PEG) 1-3%; enzymes (calculated as pure enzyme protein) 0.0001-0.1%; and minority ingredients (eg, foam suppressants, perfume) 0-5%.

[00105] 5) An aqueous liquid detergent composition comprising linear alkylbenzenesulfonate (calculated as acid) from about 15% to about 21%; alcohol ethoxylate (for example, C12-15, 7 EO alcohol or C12-15, 5 EO alcohol) about 12% to about 18%; soap such as fatty acid (eg oleic acid), about 3% to about 13%; alkenyl succinic acid (C12-14) 0% to about 13%; amino-ethanol about 8% to about 18%; citric acid about 2% to about 8%; phosphonate 0% to about 3%; polymers (for example, PVP, PEG) 0% to about 3%; borate (for example, B4O7) 0% to about 2%; 0% ethanol to about 3%; propylene glycol about 8% to about 14%; enzymes (calculated as pure enzyme protein) 0.0001-0.1%; and minor ingredients (eg dispersants, foam suppressants, perfume, optical brightener) 0-5%.

[00106] 6) An aqueous structured liquid detergent composition, comprising linear alkylbenzenesulfonate (calculated as acid) from about 15% to about 21%; alcohol ethoxylate (for example, C12-15, 7 EO alcohol, or C12-15, 5 EO alcohol) 3-9%; soap, such as fatty acid (eg oleic acid) about 3% to about 10%; zeolite (like NaA1SiO4) about 14% to 22%; potassium citrate about 9% to about 18%; borate (for example, B4O7) 0% to about 2%; carboxymethylcellulose (CMC) 0% to about 2%; polymers (for example, PEG, PVP) 0% to about 3%; anchoring polymers such as, for example, lauryl methacrylate / acrylic acid copolymer; molar ratio 25: 1, PM 3800) 0% to about 3%; 0% glycerol to about 5%; enzymes (calculated as pure enzyme protein) 0.0001-0.1%; and minority ingredients (eg dispersants, foam suppressants, perfume, optical brighteners) 0-5%.

[00107] 7) A detergent composition formulated as a granulate containing an apparent density of at least 600 g / L comprising fatty alcohol sulfate from about 5% to about 10%; ethoxylated fatty acid monoethanolamide from about 3% to about 9%; soap like 0-3% fatty acid; sodium carbonate (for example, Na2CO3) about 5% to about 10%; soluble silicate (for example, Na2O, 2SiO2) about 1% to about 4%; zeolite (for example, NaA1SiO4) about 20% to about 40%; sodium sulfate (for example, Na2SO4) about 2% to about 8%; sodium perborate (for example, NaBO3H2O) about 12% to about 18%; TAED about 2% to about 7%; polymers (for example, maleic / acrylic acid copolymer, PEG) about 1% to about 5%; enzymes (calculated as pure enzyme protein) 0.0001-0.1%; and minor ingredients (eg, optical brightener, foam suppressants, perfume) 0-5%.

[00108] 8) A detergent composition formulated as a granulate comprising linear alkylbenzenesulfonate (calculated as acid) from about 8% to about 14%; ethoxylated fatty acid monoethanolamide about 5% to about 11%; soap like 0% fatty acid to about 3%; sodium carbonate (for example, Na2CO3) about 4% to about 10%; soluble silicate (Na2O, 2SiO2) about 1% to about 4%; zeolite (for example, NaA1SiO4) about 30% to about 50%; sodium sulfate (for example, Na2SO4) about 3% to about 11%; sodium citrate (for example, C6H5Na3O7) about 5% to about 12%; polymers (e.g., PVP, acrylic maleic acid copolymer, PEG) about 1% to about 5%; enzymes (calculated as pure enzyme protein) 0.0001-0.1%; and minority ingredients (eg, foam suppressants, perfume) 0-5%.

[00109] 9) A detergent composition formulated as a granulate comprising linear alkylbenzenesulfonate (calculated as acid), about 6% to about 12%; nonionic surfactant, about 1% to about 4%; soap as fatty acid, about 2% to about 6%; sodium carbonate (for example, Na2CO3), about 14% to about 22%; zeolite (for example, NaA1SiO4), about 18% to about 32%; sodium sulfate (for example, Na2SO4), about 5% to about 20%; sodium citrate (for example, C6H5Na3O7), about 3% to about 8%; sodium perborate (for example, NaBO3H2O), about 4% to about 9%; bleach activator (for example, NOBS or TAED) about 1% to about 5%; carboxymethylcellulose (CMC), about 0% to about 2%; polymers (for example, polycarboxylate or PEG), about 1% to about 5%; enzymes (calculated as pure enzyme protein), 0.0001-0.1%; and minority ingredients (eg optical brightener, perfume) 0-5%.

[00110] 10) An aqueous liquid detergent composition comprising linear alkylbenzenesulfonate (calculated as acid), about 15% to about 23%; alcohol ethoxysulfate (for example, C12-15 alcohol, 2-3 EO) about 8% to about 15%; alcohol ethoxylate (for example, C12-15, 7 EO alcohol, or C12-15, 5 EO alcohol) about 3% to about 9%; soap such as fatty acid (eg lauric acid) 0% to about 3%; aminoethanol, about 1% to about 5%; sodium citrate, about 5% to about 10%; hydrotrope (for example, sodium toluenesulfonate) about 2% to about 6%; borate (for example, B4O7), 0% to about 2%; carboxymethylcellulose, 0% to about 1%; ethanol, about 1% to about 3%; propylene glycol, about 2% to about 5%; enzymes (calculated as pure enzyme protein) 0.0001-0.1%; and minority ingredients (for example, polymers, dispersants, perfume, optical brighteners), 0-5%.

[00111] 11) An aqueous liquid detergent composition comprising linear alkylbenzenesulfonate (calculated as acid), from about 20% to about 32%; alcohol ethoxylate (for example, C12-15, 7 EO alcohol, or C12-15, 5 EO alcohol), 6-12%; aminoethanol, about 2% to about 6%; citric acid, about 8% to about 14%; borate (for example, B4O7) about 1% to about 3%; polymer (for example, maleic acid / acrylic copolymer, anchoring polymer such as, for example, acrylic acid / methacrylate lauryl copolymer), 0% to about 3%; glycerol, about 3% to about 8%; enzymes (calculated as pure enzyme protein) 0.0001-0.1%; and minority ingredients (eg hydrotropes, dispersants, perfume, optical brighteners), 0-5%.

[00112] 12) A detergent composition formulated as a granulated granule having a specific density of at least 600 g / L comprising anionic surfactant (linear alkylbenzenesulfonate, alkyl sulfate, α-olefinsulfonate, methyl esters of α-sulfo fatty acid (alkanesulphonates, soap), about 25% to about 40%; nonionic surfactant (eg, alcohol ethoxylate), about 1% to about 10%; sodium carbonate (for example, Na2CO3), about 8% to about 25%; soluble silicates (for example, Na2O, 2SiO2) about 5% to about 15%; sodium sulfate (for example, Na2SO4), 0% to about 5%; zeolite (NaA1SiO4), about 15% to about 28%; sodium perborate (for example, NaBO3.4H2O), 0% to about 20%; bleach activator (TAED or NOBS) about 0% to about 5%; enzymes (calculated as pure enzyme protein) 0.0001-0.1%; and minority ingredients (eg perfume, optical brighteners) 0-3%.

[00113] 13) Detergent compositions as described in positions 1) to 12) above, in which all or part of the linear alkylbenzenesulfonate is replaced by alkyl sulfate (C12-C18).

[00114] 14) A detergent composition formulated as a granulate having a specific density of at least 600 g / L comprising alkyl sulfate (C12-C18), about 9% to about 15%; alcohol ethoxylate about 3% to about 6%; polyhydroxy alkyl fatty acid amide, about 1% to about 5%; zeolite (for example, NaA1SiO4), about 10% to about 20%; layered disilicate (e.g., Hoechst SK56) about 10% to about 20%; sodium carbonate (for example, Na2CO3) about 3% to about 12%; soluble silicate (for example, Na2O, 2SiO2), 0% to about 6%; sodium citrate, about 4% to about 8%; sodium percarbonate, about 13% to about 22%; TAED, about 3% to about 8%; polymers (for example, polycarboxylates and PVP), 0% to about 5%; enzymes (calculated as pure enzyme protein) 0.0001-0.1%; and minority ingredients (for example, optical brightener, photobleacher, perfume, foam suppressants), 0-5%.

[00115] 15) A detergent composition formulated as a granulate having a specific density of at least 600 g / L comprising alkyl sulfate (C12-C18), about 4% to about 8%; alcohol ethoxylate, about 11% to about 15%; soap, about 1% to about 4%; MAP zeolite or zeolite A, about 35% to about 45%; sodium carbonate (such as Na2CO3) about 2% to about 8%; soluble silicate (for example, Na2O, 2SiO2), 0% to 4%; sodium percarbonate, about 13% to about 22%; TAED, 1-8%; carboxymethylcellulose (CMC), 0% to about 3%; polymers (for example, polycarboxylates and PVP), 0% to about 3%; enzymes (calculated as pure enzyme protein) 0.0001-0.1%; and minority ingredients (eg optical brightener, phosphonate, perfume) 0-3%.

[00116] 16) Detergent formulations as described in 1) to 15) above, which contain a stabilized or encapsulated peracid, as an additional component or as a substitute for the already specified bleaching systems.

[00117] 17) Detergent compositions as described above in 1), 3), 7), 9) and 12), in which the perborate is replaced by percarbonate.

[00118] 18) Detergent compositions as described above in 1), 3), 7), 9), 12), 14) and 15), which additionally contains a manganese catalyst. The manganese catalyst, for example, is one of the compounds described in "Efficient manganese catalysts for low-temperature bleaching," Nature 369: 637-639 (1994).

[00119] 19) Detergent composition formulated as a non-aqueous liquid detergent comprising a liquid non-ionic surfactant, such as, for example, linear alkoxylated primary alcohol, a reinforcing agent system (eg, phosphate), an enzyme (s) and alkali. The detergent may also comprise anionic surfactant and / or a bleaching system.

[00120] As above, the present enzyme can be incorporated in a concentration conventionally used in detergents. It is presently contemplated that, in the detergent composition, the enzyme can be added in an amount corresponding to 0.000011.0 mg (calculated as pure enzyme protein) of the enzyme polypeptide per liter of washing liquor.

[00121] The detergent composition may also contain other conventional detergent ingredients, for example, deflocculating material, filler material, foam suppressants, anti-corrosion agents, impurity suspending agents, sequestering agents, impurity anti-redeposition agents, dehydrating agents, dyes, bactericides, fluorescents, thickeners and perfumes.

[00122] The detergent composition can be formulated as a detergent composition for washing clothes by hand (manual) or by machine (automatic), including an additive composition for washing clothes suitable for pretreating stained fabrics and a composition of fabric softener added with rinse, or be formulated as a detergent composition for use in general household hard surface cleaning operations, or be formulated for manual or automatic dish washing operations.

[00123] Any of the cleaning compositions described here in this document can include any number of additional enzymes. In general, the enzymes must be compatible with the selected detergent, (for example, in relation to the ideal pH, compatibility with other enzymatic and non-enzymatic ingredients and the like), and the enzyme (s) must be present (s) in effective amounts. The following enzymes are provided as examples.

[00124] Proteases: Suitable proteases include those of animal, vegetable or microbial origin. Chemically modified or engineered mutations for protein are included, as well as naturally processed proteins. The protease can be a serine protease or a metalloprotease, an alkaline microbial protease, a trypsin-like protease or a chymotrypsin-like protease. Examples of alkaline proteases are subtilisins, for example, those derived from Bacillus, for example, subtilisin Novo, subtilisin Carlsberg, subtilisin 309, subtilisin 147, and subtilisin 168 (see, for example, WO 89/06279). Examples of trypsin-like proteases are trypsin (for example, of porcine or bovine origin), and Fusarium proteases (see, for example, Patent WO 89/06270 and WO 94/25583). Examples of useful proteases also include but are not limited to variants described in WO 92/19729, WO 20115/98, WO 98/20116 and WO 98/34946. Commercially available protease enzymes include, but are not limited to: ALCALASE®, SAVI-NASE®, PRIMASE®, DURALASE®, ESPERASE®, KANNASE®, and BLAZE® (Novo Nordisk A / S and Novozymes A / S); MAXATASE®, MA-XACAL®, MAXAPEM®, PROPERASE®, PURAFECT®, PURAFECT OXP®, FN2®, and FN3® (Danisco US Inc.). Other exemplary proteases include NprE from Bacillus amyloliquifaciens and ASP from Cellulomonas sp., Strain 69B4.

[00125] Lipases: Suitable lipases include those of bacterial or fungal origin. Chemically modified, proteolytically modified, or protein engineered mutants are included. Examples of useful lipases include but are not limited to Humicola lipases (synonym Thermomyces), for example, from H. lanuginosa (T. lanuginosus) (see for example, Patent EP 258068 and EP 305216), from H. insolens (see for example, Patent WO 96/13580); a Pseudomonas lipase (for example, from P. alkaliens or P. pseudoalalkigenes; see, for example, EP 218 272), P. cepacia (see, for example, EP 331 376), P. stutzeri (see, for example) , GB 1,372,034), P. fluorescens, Pseudomonas sp. strain SD 705 (see, for example, WO 95/06720 and WO 96/27002), P. wisconsinensis (see, for example, WO 96/12012); a Bacillus lipase (for example, from B. subtilis; see for example, Dartois et al. Biochemica et Biophysica Acta, 1131: 253-360 (1993)), B. stearothermophilus (see, for example, JP Patent 64 / 744992), or B. pumilus (see for example, Patent WO 91/16422). Additional lipase variants contemplated for use in formulations include those described for example in: WO 92/05249, WO 01541/94, WO 95/35381, WO 96/00292, WO 95/30744, WO 94/25578, WO 95 / 14783, WO 95/22615, WO 97/04079, WO 97/07202, EP 407225 and EP 260105. Some commercially available lipase enzymes include LIPOLASE® and LIPOLASE ULTRA® (Novo Nordisk A / S and Novozymes A / S).

[00126] Polyesterases: Suitable polyesterases can be included in the composition, such as those described in, for example, WO 01/34899, WO 01/14629, and US6933140.

[00127] Amylases: The compositions can be combined with amylases, such as improved non-production amylase. These may include commercially available amylases, such as, but not limited to STAINZYME®, NATALASE®, DURAMYL®, TERMAMYL®, FUN-GAMYL® and BAN® (Novo Nordisk A / S and Novozymes A / S); RAPIDA-SE®, POWERASE®, and PURASTAR® (from Danisco US Inc.).

[00128] Cellulases: Cellulases can be added to the compositions. Suitable cellulases include those of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Suitable cellulases include cellulases of the genera Bacillus, Pseudomonas, Humicola, Fusarium, Thielavia, Acremonium, for example, fungal cellulases produced from Humicola insolens, Myceliophthora thermophila and Fusarium oxysporum disclosed, for example, in US Patent No. 4,435,307; 5,648,263; 5,691,178; 5,776,757; and WO 89/09259. Examples of cellulose contemplated for use are those that contain the benefit of color care for the textile product. Examples of such cellulases are the cellulases described for example in EP 0495257, EP 0531372, WO 11262/96, WO 96/29397 and WO 98/08940. Other examples are cellulase variants, such as those described in WO 94/07998; WO 98/12307; WO 95/24471; PCT / DK98 / 00299; EP 531315; US Patent No. 5,457,046; 5,686,593; and 5,763,254. Commercially available cellulases include CELLUZYME® and CAREZYME® (Novo Nordisk A / S and Novozymes A / S); and PURADAX HA® (Danisco US Inc.); and KAC-500 (B) ® (Kao Corporation).

[00129] Peroxidases / Oxidases: Suitable peroxidases / oxidases contemplated for use in the compositions include those of plant, bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Examples of useful peroxidases include Coprinus, for example, C. cinereus peroxidases, and variants thereof such as those described in WO 24618/93, WO 95/10602 and WO 98/15257. Commercially available peroxidases include, for example, GUARDZYME® (Novo Nordisk A / S and Novozymes A / S).

[00130] The detergent composition can also comprise 2,6-β-D-fructan hydrolase, which is effective for removing / cleaning biofilm present in the home and / or industrial textiles / laundries.

[00131] Detergent enzymes can be included in a detergent composition by adding separate additives containing one or more enzymes, or by adding a combined additive comprising all of these enzymes. A detergent additive, that is, a separate additive or a combined additive, can be formulated, for example, as a granulate, a liquid, a slurry and the like. Examples of detergent additive formulations include, but are not limited to, granules, in particular, non-dusting granules, liquids, in particular stabilized liquids or slurries.

[00132] Powdered granules can be produced, and added with the present granule compositions for example, as disclosed in US Patent Nos. 4,106,991 and 4,661,452 and can optionally be coated by methods known in the art. Examples of waxy coating materials are poly (ethylene oxide) products (for example, polyethylene glycol, PEG) with average molar weights of 1,000 to 20,000; ethoxylated nonylphenols containing from 16 to 50 ethylene oxide units; ethoxylated fatty alcohols in which the alcohol contains 12 to 20 carbon atoms and in which there are 15 to 80 ethylene oxide units; fatty alcohols; fatty acids; and mono- and die fatty acid triglycerides. Examples of film-forming coating materials suitable for application using fluid bed techniques are given, for example, in Patent GB 1483591. Liquid enzyme preparations can, for example, be stabilized by the addition of a polyol such as propylene glycol , a sugar or sugar alcohol, lactic acid or boric acid, according to established methods.

[00133] The detergent composition can be in any convenient form, for example, a bar, a tablet, a powder, a granulate, a paste or a liquid. A liquid detergent can be aqueous, normally containing up to about 70% water and 0% to about 30% organic solvent. Compact detergent gels containing about 30% or less of water are also contemplated. The detergent composition can optionally comprise one or more surfactants, which can be non-ionic, including semi-polar and / or anionic and / or cationic or zwitterionic. Surfactants can be present in a wide range, from about 0.1% to about 60% by weight.

[00134] When included the detergent will normally contain from about 1% to about 40% of an anionic surfactant, such as linear alkylbenzenesulfonate, α-olefinsulfonate, alkyl sulfate (fatty alcohol sulfate), ethoxysulfate alcohol, secondary alkanesulfonate, α-sulfo fatty acid methyl ester, alkyl- or alkenyl succinic acid, or soap.

[00135] When included, the detergent will generally contain from about 0.2% to about 40% of nonionic surfactant such as ethoxylated alcohol, ethoxylated nonylphenol, alkyl polyglycoside, alkyldimethylamino oxide, ethoxylated fatty acid monoethanol, mono ethanolamide of fatty acid, polyhydroxy amide alkyl fatty acid or N-acyl-N-alkyl glucosamine derivatives ("glycamides").

[00136] The detergent may contain 0% to about 65% of a detergent-reinforcing agent or complexing agent such as zeolite, diphosphate, triphosphate, phosphonate, carbonate, citrate, nitrilotriacetic acid, ethylenediamine tetraacetic acid (EDTA), diethylenetriamineapenta-cetic acid, alkyl- or alkenyl succinic acid, soluble silicates or layered silicates (eg SKS-6 from Hoechst).

[00137] The detergent may comprise one or more polymers. Examples of polymers include carboxymethylcellulose (CMC), polyl (vinylpyrrolidone) (PVP), poly (ethylene glycol) (PEG), poly (vinyl alcohol) (PVA), poly (vinylpyridine-N-oxide), poly (vinylimidazole) , polycarboxylate, for example, polyacrylates, maleic acid / acrylic copolymers) and lauryl methacrylate / acrylic acid copolymers.

[00138] The enzymes of the detergent composition can be stabilized using conventional stabilizing agents, for example, such as polyol (for example, propylene glycol or glycerol), a sugar or sugar alcohol, lactic acid, boric acid, or a boric acid derivative ( for example, an aromatic borate ester), or a derivative of phenyl boronic acid (for example, 4-formylphenyl boronic acid). The composition can be formulated as described in WO 92/19709 and WO 92/19708.

[00139] It is contemplated that in detergent compositions containing enzymes, an amount corresponding to about 0.01 to about 100 mg of enzyme protein per liter of washing liquor can be added (for example, about 0.05 up to about 5.0 mg of enzyme protein per liter of washing liquor or 0.1 to about 1.0 mg of enzyme protein per liter of washing liquor.

[00140] Although the present compositions and methods have been described with reference to the details below, it should be understood that several modifications can be made.

[00141] Various enzyme cleaning assays are known in the art, including sample and micro-sample assays. BEER MANUFACTURING COMPOSITIONS

[00142] The present granule composition can be a component of a brewing composition used in a fermented beverage delivery process, such as brewing. Non-fermentable carbohydrates are believed to form the majority of solids dissolved in the final beer. This residue remains due to the inability of the malt amylases to hydrolyze the alpha-1,6 bonds of the starch. Non-fermentable carbohydrates contribute about 50 calories for about 340 grams (12 ounces) of beer. The present granule composition, usually, in combination with a glycoamylase and optionally, a pullulanase and / or isoamylase, aid in the conversion of starch into dextrins and fermentable sugars, reducing residual non-fermentable carbohydrates in the final beer.

[00143] The main raw materials used to produce these drinks are water, hops and malt. In addition, but not exclusively, auxiliary compounds, such as common corn kernels, refined corn kernels, brewer's milled yeast, rice, sorghum, refined corn starch, barley, barley starch, husked barley, wheat, wheat starch. wheat, roasted cereals, cereal flakes, rye, oats, potatoes, tapioca, and syrups, such as corn syrup, sugar cane syrup, inverted sugar syrup, barley and / or wheat syrups, and the like can be used as a source of starch.

[00144] For a number of reasons, malt, which is produced mainly from selected varieties of barley, has an important effect on the general character and quality of beer. First, malt is the main flavoring agent in beer. Second, malt provides the largest portion of fermentable sugar. Third, malt provides the proteins, which will contribute to the beer's body and foam characteristics. Fourthly, malt provides the necessary enzyme activity during crushing. Hops also contribute significantly to beer quality, including flavor. In particular, hops (or constituents of hops) add desirable bitter substances to beer. In addition, hops can act as a protein precipitant, establish preservative agents and aid in the formation and stabilization of foam.

[00145] Cereals (grains), such as barley, oats, wheat, but also corn and rice are often used for brewing, both in industry and in the manufacture of homemade beer, but also other vegetable components such as hops are often added. Components used in brewing can be unmalted or can be malted, that is, partially germinated, resulting in an increase in enzyme levels, including α-amylase. For successful brewing, adequate levels of α-amylase enzyme activity are needed to ensure adequate levels of sugars for fermentation. The present granule composition can also be added to components used for brewing.

[00146] As used herein, the term "stock" means grains and plant components that are crushed or broken. For example, the barley used in the production of beer is a grain that is coarsely ground or crushed to produce a suitable consistency for the production of a paste for fermentation. As used herein, the term "stock" includes any of the aforementioned types of plants and grains in crushed or coarsely ground forms. The methods described here in this document can be used to determine levels of α-amylase activity in flours and stock.

[00147] Processes for producing beer are well known in the art. See, for example, Wolfgang Kunze (2004) "Technology Brewing and Malting," Research and Teaching Institute of Brewing, Berlin (VLB), 3rd edition. In summary, the process involves: (a) preparing a paste, (b) filtering the paste to prepare a wort, and (c) fermenting the wort to obtain a fermented drink, such as beer. Typically, crushed or crushed malt, malt and auxiliary compound, or auxiliary compound is mixed with water and maintained for a period of time under controlled temperatures to allow the enzymes present in the malt and / or the auxiliary compound to convert the starch present in the malt. in fermentable sugars. The paste is then transferred to a paste filter where the liquid is separated from the grain residue. This sweet liquid is called "must", and grain residue left is called "used grain". The pulp is typically subjected to extraction, which involves adding water to the pulp to recover the residual soluble grain extract used. The wort is then boiled vigorously to sterilize the wort and help to develop the color, flavor and odor. Hops are added at some point during the boil. The must is cooled and transferred to a fermenter.

[00148] The must is then placed in contact with the yeast in a fermenter. The fermenter can be refrigerated to stop fermentation. The yeast that can flocculate is removed. Finally, the beer is cooled and stored for a period of time, during which the beer clarifies and develops its flavor, and any material that may impair the appearance, taste and shelf life of the beer settles down. Beer generally contains about 2% to about 10% v / v alcohol, although beer with a higher content, for example, 18% v / v alcohol, can be obtained. Before being packaged, the beer is carbonated and, optionally, filtered and pasteurized.

[00149] The composition for brewing, comprising an alpha-amylase, often, but not necessarily, in combination with one or more exogenous enzymes, such as glycoamylase (s), pullulanase (s) and / or isoamylase (s) , and any combination of these, can be added to the paste from step (a) above, that is, during the preparation of the paste. Alternatively, or in addition, the brewing composition can be added to the pulp from step (b) above, as during filtering the pulp. Alternatively, or in addition, the brewing composition can be added to the wort from step (c) above, as during wort fermentation.

[00150] Particular modalities belong to any of the above uses, methods or fermented beverages, in which said fermented drink is a beer, such as fully malted beer, beer brewed under "Reinheitsgebot" grade, ale, IPA, Lager, bitter, Happoshu ( second beer), third beer, dry beer, almost beer, light beer, low alcohol beer, low calorie beer, porter, bock beer, stout, malt liquor, non-alcoholic beer, non-alcoholic malt liquor and the like, but alternative cereals and malt beverages such as fruit-flavored malted beverages, for example, with citrus flavor, such as lemon, orange, lime, or cherry malted beverages, liquor-flavored malted beverages, for example, malt liquor with flavored vodka, rum, or tequila, or malted drinks with a coffee flavor, such as caffeine-flavored malt liquor, and the like. ADDITIONAL ANIMAL FEED MODALITIES

[00151] In some embodiments, especially in the context of animal feed applications, the granules of the present teachings can be used with any of a variety of at least one of the following enzymes:

[00152] Only as an example, chicken feed, for example broilers, may comprise one or more of the ingredients listed in the table below, for example, in the% for age given in the table below:

[00153] Just as an example, the dietary specification for chickens, such as a broiler, can be as set out in the Table below:

[00154] Just as an example, diets for laying hens can comprise one or more of the ingredients listed in the table below, for example, in the% for age given in the table below:

[00155] Just as an example, the dietary specification for laying hens can be as set out in the Table below:

[00156] Just as an example, turkey feed may comprise one or more of the ingredients listed in the table below, for example, in the% for age given in the table below:

[00157] Just as an example, the dietary specification for turkeys can be as set out in the Table below:

[00158] Just as an example, piglet rations may comprise one or more of the ingredients listed in the table below, for example, in the% for age given in the table below:

[00159] Just as an example, the dietary specification for piglets can be as set out in the Table below:

[00160] Just as an example, diets for growing / ready-for-slaughter pigs may comprise one or more of the ingredients listed in the table below, for example, in the% for age given in the table below:

DOSAGEM DA RAÇÃO[00161] Just as an example, the dietary specification for growing / ready-for-slaughter pigs can be as set out in the Table below:

RATION DOSAGE

[00162] The feed additive composition according to the present invention can be planned for single dosing or can be planned for feeding on a daily basis.

[00163] The optimum amount of the composition (and each component in it) to be used in the combination of the present invention will depend on the product to be treated and / or the method of putting the product in contact with the composition and / or the use intended for it.

[00164] The amount of enzymes used in the compositions must be sufficient to be effective and remain sufficiently effective in improving the performance of the feed products given to the animal containing said composition. The period of time for effectiveness must extend to at least the time of use of the product (for example, additive feed composition or feed containing the same). COMBINATION WITH OTHER COMPONENTS IN FOOD APPLICATIONS

[00165] The enzymes described here can be used in combination with any other components.

[00166] The components can be prebiotics. Prebiotics are typically non-digestible carbohydrates (oligo - or polysaccharides) or a sugar alcohol that is not degraded or absorbed in the upper digestive tract. Known prebiotics used in commercial and useful products according to the present invention include inulin (fructo-oligosaccharide, or FOS) and transgalacto-oligosaccharide (GOS or TOS). Suitable prebiotics include palatinose oligosaccharide, soy oligosaccharide, alginate, xanthan gum, pectin, alfalfa robin (LBG), inulin, guar gum, galacto-oligosaccharide (GOS), fructo-oligosaccharide (FOS), non-degradable starch , lactose sucrose, lactulate, lactitol, maltitol, maltodextrin, polydextrose (ie Litesse ®), lactitol, lactose sucrose, soy oligosaccharides, palatinose, isomalto-oligosaccharides, glyco-oligosaccharides and xylo-oligosaccharides.

[00167] Prebiotics can be administered simultaneously with (for example, mixed together with, or administered simultaneously by the same or different routes) or sequentially to (for example, the same or different routes) feed additive composition (or constituents thereof) ) according to the present invention.

[00168] Other components of the combinations of the present invention include polydextrose, such as Litesse®, and / or a maltodextrin and / or lactitol. These other components can optionally be added to the feed additive composition to aid the drying process.

[00169] Other examples of other suitable components include one or more of: thickeners, gelling agents, emulsifiers, binders, crystal modifiers, sweeteners (including artificial sweeteners), rheology modifiers, stabilizers, anti-oxidants, dyes, enzymes, carriers , vehicles, excipients, thinners, lubricating agents, flavoring agents, coloring materials, suspending agents, disintegrants, granulating binders, etc. These other components can be natural. These other components can be prepared using chemical and / or enzymatic techniques.

[00170] In a preferred embodiment the enzymes for use in the present invention can be used in combination with one or more lipids.

[00171] For example, enzymes for use in the present invention can be used in combination with one or more lipid micelles. The lipid micelle can be a simple lipid micelle or a complex lipid micelle.

[00172] The lipid micelle can be an aggregate of oriented molecules of amphipathic substances, such as a lipid and / or an oil.

[00173] The present teachings also provide a method to increase weight gain in an individual, for example, poultry or pigs, comprising feeding said individual with a feed comprising a feed additive composition according to present invention.

[00174] The invention can be better understood by reference to the following Examples, which are provided by way of illustration, and are not intended to be limiting. EXAMPLES: EXAMPLE 1 Particle size distribution and smoothness index of sodium sulfate cores.

[00175] Seven samples of crystalline anhydrous sodium sulfate core particles were obtained from two suppliers: Minera de Santa Marta (Villarubio, Spain) and Saltex (Forth Worth, Texas),

EXEMPLO 2 Produção de uma amostra de grânulos de enzima detergentes utilizando núcleos de sulfato de sódio.[00176] The particle size distributions of the samples were obtained by laser light scattering using a Malvern Mastersizer 2000 laser diffraction particle size analyzer. The envelope specific surface areas for these particle size distributions were calculated using an algorithm as described in the methods section, using the true density of anhydrous sodium sulfate (2,664 g / cm3). The specific surface area BET was determined using a Surface Area System and Accelerated Porosimetry from Micromeritics model ASAP 2420, as described in the methods section. The smoothness index for each sample is calculated as the ratio between these two specific surface areas. Representative images for two of the nucleus lots are shown in Figure 1. TABLE 1

EXAMPLE 2 Production of a sample of detergent enzyme granules using sodium sulfate cores.

[00177] 79.8 kg of sodium sulphate cores available from Minera de Santa Marta (MSM Batelada # 3 in Table 1 of Example 1) were loaded into a batch scale pilot scale fluidized bed coating application device of 150 kg. By light scattering, the nuclei had a mass median diameter (D50) of 258 microns, a D10 of 182 microns and a D90 of 358 microns; by scanning through a molecular sieve, a 210 micron D50. Based on this, MSM Lot # 3 had a PSDI of 362/182 = 1.99.

[00178] According to Table 1 of Example 1, the smoothness index of the MSM Lot # 3 cores was 1.1, very close to the smoothness index 1.0 of perfectly smooth particles. Scanning electron and optical micrographs of the cores are shown in Figure 1. It can be seen in the same figure that these cores are much smoother than a comparative sample of Saltex cores. Table 1 shows that Saltex Lote # 1 had a smoothness index of 3.9, indicating a significant deviation from a perfect smoothness index of 1.0.

[00179] The cores were fluidized in a bed at a temperature of 41 degrees C, and an aqueous solution of enzyme protease (Purefast) at a concentration of 20% w / w solids (3450 PU / g), containing 1 % w / w of poly (vinyl alcohol) partially hydrolyzed (% w / w of final solids enzyme load / 16% PVA) and 0.5% w / w of antifoam (Polyglycol EP 436E (lot 1L 05091501) was sprayed onto the cores at an atomizing air pressure rising from 3.5 (initial) to 3.9 (final) bar.

[00180] After the enzyme spray was completed, a solution containing a mixture of sodium sulfate and magnesium sulfate (MgSO4 7H 20, 30.5%, Na2SO4, 15%) was sprayed onto the cores to provide 20 % w / w, liquid - based on the final granule weight - to the cores. The salt solution was sprayed at a bed temperature of 45 degrees C, with an atomizing air pressure of 0.35 MPa (3.5 bar).

[00181] A final coating solution composed of partially hydrolyzed poly (vinyl alcohol) (Celvol 5-88), titanium dioxide and non-ionic Lutensol surfactant, was sprayed on the enzyme nuclei coated with salt, to provide a weight of 11% w / w net total coating. The coating solution was sprayed at a bed temperature of 55 degrees C, with an atomizing air pressure of 0.53 MPa (5.3 bar), dropping to 0.48 Mpa (4.8 bar).

[00182] After cooling the batch of coated granules, 150 kg of product were collected from the fluidized bed coating application device. The final granules had a D50 of 308 µm (by molecular sieve analysis) and a specific density of 1.18 g / cm3. The granules had a Hunter whiteness L value of 73, and total enzyme dust per Heubach of 3.2 mg / pad. EXAMPLE 3

[00183] Four additional batches of Purafast granules were prepared, following the recipe and the procedure described in Example 2, changing only the amount of Purafast enzyme concentration solution coated on the nuclei, the particle size of the nucleus, and adjustment of the core loading as needed to accommodate different levels of enzyme solids. The additional coatings and process parameters have not been changed.

[00184] The enzyme samples below were tested for storage in a Laundry Detergent Powder under different stress storage conditions. Stability was assessed through performance and biochemical analyzes. STORAGE TEST: SAMPLE PREPARATION

[00185] Corresponding amounts of enzyme (see below) were added to 10 g of a Laundry Detergent, and placed in plastic cups (150 mL), which were stored, open, at 70% relative humidity in acclimatized rooms. at 37, 45 and 60 ° C. A. 10 g of Laundry Detergent + Sample A1 B. 10 g of Laundry Detergent + Sample B1 C. 10 g of Laundry Detergent + Sample B2 D. 10 g of Laundry Detergent + Sample C1 E. 10 g of Laundry Detergent + Sample C2

[00186] The samples were analyzed fresh and after storage for 1 and 2 hours, and 3 days (at 60 ° C); and after 1, 3 and 8 days (at 37 and 45 ° C) PERFORMANCE ASSESSMENT.

[00187] The Wash Test was performed on the so-called Laundr-O-meter at 30 C, 25 FH and 15 minutes.

[00188] The 10 g stored sample was dissolved in 2.5 liters of 25 ° FH water (see below) in a beaker with stirring for 2 minutes. 300 mL of this solution is placed in a LOM beaker and the stains below have been added to give a L / C ~ 90 liquor to cloth ratio (without the steel balls).

[00189] To make 25 L of 25 ° FH = 250 ppm Ca + 2 / Mg + 2 = 2/1, dilute 416.75 mL of a 15,000 ppm stock solution with 24583.25 mL of deionized water (to make 25 liters) of 15,000 ppm stock solution: 14.70 grams of CaCl2.2H2O + 10.165 grams of MgCl2.6H2O in 1 liter of DI water.

[00190] Beaker stains (initial reflectance [L * a * b, illuminant D65] of samples previously measured with the reflectometer):

[00191] After washing, the stains were rinsed for 3 minutes in running tap water (17 FH, Ca / Mg = 4/1), dried by rotation and air dried under ambient conditions overnight (covered with dark clothes).

[00192] Triple measurements of the final reflectance [L * a * b, illuminant D65] of the samples were performed using the reflectometer. Delta Dust Removal% (% dE) is calculated according to:

[00193] Where Lref = 96.0

[00194] aref = 0.55

[00195] bref = 1.95

BIOCHEMICAL EVALUATION

[00196] This method was used to determine the proteolytic activity in fermentation, recovery and samples of the final product (liquids and granules) of Properase. The assay is colorimetric and monitors the degradation ratio of substrate N-succinyl-ala-ala-pro-phe-p-nitroanalide. The release of p-notroanilide from the substrate was measured at 405 nm on a Konelab analyzer. The assay is calibrated against a designated standard. SAMPLE PREPARATION:

[00197] - 100 g of Tris Buffer (0.1 M TRIS, CaCl2, 0.01 M, Triton X-100, 0.005%, pH 8.6) were added to 10 g of the detergent sample samples, and stirred for 30 minutes. Subsequently, 2 mL of the previous dissolution was taken in an eppendorf tube and centrifuged for 10 minutes at 14000 rpm.

[00198] - A second dissolution (10X) was made using the HAMILTON diluting machine and Tris buffer.

[00199] - The samples were vortexed after dilution, and poured into the corresponding cuvettes for Konelab. Controls were also included (in this case pU / g). The cuvettes were placed on a sample holder (s). The holder (s) were inserted into the instrument. TO RUN THE KONELAB TEST:

[00200] - The working substrate solution [N-succinyl-ala-ala-pro-phe-p-nitroanalide (AAPF) (Sigma # S7388)] was prepared and poured into a reagent container and placed in a reagent carousel (substrate = 20 ml container, code = AAPF). REAGENT PREPARATION

[00201] 1. Stock solution (100 mg / mL): Dissolve 500 mg of AAPF in 5 mL of DMSO. Stored at room temperature protected from light. Discard if the solution turns yellow. Stable for 1 month.

[00202] 2. Intermediate substrate solution (20 mg / ml): Add 800 µl of substrate stock solution to 3.2 mL of DMSO and mix. Stored at room temperature protected from light. Discard if the solution turns yellow. Stable for 1 month.

[00203] 3. Working substrate solution (1 mg / ml): Add 250 µl of intermediate substrate solution to 4.75 ml of assay buffer and mix. Prepared as needed. Typically stable for 1 hour if stored on ice. Discard if the solution turns yellow.

[00204] - The sampling cups were identified in the computer program and the test to be run on the Konelab (six replicates) was defined. Run and check the calibration. To calculate the activity of the granular samples (This equation assumes that 1 g = 1 mL, as the specific gravity of the dissolution mixture): PU / g granule = [(PU / ml) * (working dilution)] * [weight dissolution buffer + granules (ml) / granule weight (g)]

[00205] - The average value was calculated for the replicates of each sample. The% CV (coefficient of variance) was calculated.

[00206] In this Example, the "Mini" granules are those that contain small smooth cores according to the present teachings.

[00207] The data are shown in Figure 2. EXAMPLE 4 Production of three representative batches of phytase

[00208] 45 kg of sodium sulfate cores available from Mi nera de Santa Marta (MSM Batelada 41418.B) were loaded into a 150 kg batch scale pilot bed fluidized bed application. By light scattering, the nuclei had a mass median diameter of 199 microns.

[00209] The cores were fluidized, and an aqueous solution of phytase enzyme (Batch 488115613) at a concentration of 17.5% w / w of solids, containing 2% w / w of partially hydrolyzed poly (vinyl alcohol) ( Celvol 5-88) and 0.5% w / w sodium phytase solution (Dr. Stra- etmans; Dermofeel PA-3; Lot NA2062), was sprayed on the cores.

[00210] After spraying the enzyme, a solution containing sodium sulfate (MSM, lot 13130405) was sprayed on the cores to provide 50% w / w, liquid - based on the final granule weight.

[00211] A final coating solution composed of partially hydrolyzed poly (vinyl alcohol) (Celvol 5-88), and talc (Microtalc FC CG-AW, lot 13/00328), was sprayed on the enzyme cores coated with salt, to provide a total net coating weight of 9%.

[00212] After cooling the batch of coated granules, 136 kg, 140 kg and 134 kg of product were collected from the fluidized bed coating application device. By light scattering, the final granules had an D50 of 362 μm, on average, and specific density of 1.27 g / cm3.

[00213] Figure 4 shows the pelletizing results of 3 batches of representative granules, 3115, 3116 and 3117. The testing tests were carried out at the Danish Teknological Institute. EXAMPLE 5 Production of three representative batches of phytase pattern TPT granules on a pilot scale of the same concentrated batch

[00214] 65 kg of sodium sulphate cores available from Mi nera de Santa Marta (MSM Na-G. III-EE, Lot 41235,12) were loaded into a fluidized bed coating application device. batch scale pilot scale of 150 kg. By light scattering, the cores had a mass median diameter of 290 microns. TABLE 3: Part of the MSM Na-G.III EE Product Specification.

[00215] The cores were fluidized, and an aqueous solution of phytase enzyme (Batch 488115613) at a concentration of 17.5% w / w of solids, containing 2% w / w of partially hydrolyzed poly (vinyl alcohol) ( Celvol 5-88) and 0.5% w / w sodium phytase solution (Dr. Stra- etmans; Dermofeel PA-3; Lot NA2062), was sprayed onto the cores.

[00216] After spraying the enzyme, a solution containing sodium sulfate (MSM, lot 13130405) was sprayed onto the cores to provide 40% w / w, liquid - based on the final granule weight.

[00217] A final coating solution composed of partially hydrolyzed poly (vinyl alcohol) (Celvol 5-88), and talc (Microtalc FC CG-AW, lot 13/00328), was sprayed on the enzyme nuclei coated with salt, to provide a total net coating weight of 9%.

[00218] After cooling each batch of coated granules, 151 kg, 151 kg and 150 kg of product were collected from the fluidized bed coating application device. By light scattering, the final granules had a D50 of 388 μm, on average, and specific density of 1.39 g / cm3.

[00219] The pelletization of the three standard TPT batches of representative phytase are shown in Figure 5.

Claims (18)

1. Population of granules, characterized by the fact that it comprises: a small smooth core particle, and an active agent, in which the small smooth core particles contained in this population have: a smoothness index less than 2, a mass median diameter less than 300 microns; a particle size dispersibility index less than 2 where the active agent is an enzyme. 2. Population of granules according to claim 1, characterized by the fact that the active agent is a coating of active agent around the smooth-core particle. 3. Population according to either of Claims 1 or 2, characterized by the fact that it additionally comprises at least one additional coating, wherein each of the at least one additional coating comprises at least 5% w / w of the final granule. 4. Population according to any one of claims 1 to 3, characterized in that the small smooth core particle comprises a crystalline salt or the small smooth core particle comprises a sodium sulphate crystal. 5. Population according to any one of claims 1 to 4, characterized by the fact that the enzyme is at least one among phytase, protease or amylase. 6. Population according to any one of claims 1 to 5, characterized by the fact that the small smooth-core particle comprises: a mass median diameter of less than 250 microns, less than 225 microns, less than 200 microns, less than 175 microns, less than 150 microns, less than 125 microns, or less than 100 microns; and a particle size dispersibility index less than 2, or less than 1.5. 7. Population, according to any of 1 to 6, characterized by the fact that it additionally comprises at least one additional coating, where each of the at least one additional coating comprises at least 6%, 7%, 8 %, 9%, or 10% w / w of the final granule. Granule population according to any one of claims 1 to 7, characterized by the fact that it comprises: small smooth-core particles, wherein the small smooth-core particles comprise a smoothness index less than 2, but not less than 1.25, a mass median diameter of less than 250 microns, but not less than 100 microns; a particle size dispersibility index less than 2, but not less than 1.5; an active agent coating comprising an enzyme, wherein the enzyme is at least one among a phytase, a pro-tease, or an amylase; and at least one additional coating, each of which has at least one additional coating comprising at least 7% but not more than 10% w / w of the final granule. Granule population according to any one of claims 1 to 8, characterized in that it further comprises: a first moisture barrier coating comprising a moisture barrier coating material; and a moisturizing moisturizing coating comprising a moisturizing moisturizing material around the first moisture barrier coating. 10. Population of granules according to claim 9, characterized by the fact that: (a) the moisture barrier coating material is selected from barrier polymers, proteins, lipids, fats and oils, acids fatty, and gums; and / or (b) the moisturizing moisturizing material is selected from starch, inorganic salts, and sugar. 11. Animal feed pellet or non-pelleted animal feed mixture, characterized by the fact that it comprises a population of granules, as defined in any one of claims 1 to 10. 12. Laundry detergent composition, dishwashing detergent composition, fabric treatment composition or baking composition characterized by the fact that it comprises a population of granules, as defined in any one of claims 1 to 10. 13. Method for preparing a population of granules, characterized by comprising: coating a small smooth-core particle with a coating of active agent comprising an enzyme, in which the small smooth-core particle comprises, a lower smoothness index than 2, but not less than 1.25, a mass median diameter of less than 300 microns, but not less than 150 microns, and a particle size dispersibility index less than 2.0, but not less than 1 , 5. 14. Process for producing an animal feed composition, characterized by the fact that it comprises: preparing the population of granules as defined in any one of claims 1 to 10; mixing the granules together with a food to obtain a non-pelleted mixture; and pelletize the non-pelleted mixture at a temperature of 70 ° C to 95 ° C. 15. Method of preparing a population of granules of feed additive, characterized in that it comprises preparing the population of granules as defined in any one of claims 1 to 10 and mixing the granules with an acceptable feed vehicle, diluent or excipient, and optional to pack. 16. Use of a granule population as defined in any one of claims 1 to 10, characterized by the fact that it is in a steam treatment or pelletizing process. 17. Method for improving storage stability, characterized in that it comprises preparing the granules as defined in any one of claims 1 to 10, and comparing the storage stability with a representative sample of a population of granules that are not it has a small smooth core particle. 18. Method for reducing dust, characterized in that it comprises preparing the granules as defined in any of claims 1 to 10, and comparing the dust with a representative sample of a population of granules that does not have a smooth core particle little.

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