CN105388038B - Lung tissue single channel fixer - Google Patents
Lung tissue single channel fixer Download PDFInfo
- Publication number
- CN105388038B CN105388038B CN201510663538.5A CN201510663538A CN105388038B CN 105388038 B CN105388038 B CN 105388038B CN 201510663538 A CN201510663538 A CN 201510663538A CN 105388038 B CN105388038 B CN 105388038B
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- lung tissue
- fixer
- accommodating chamber
- fixed
- wing passage
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- 210000004072 lung Anatomy 0.000 title claims abstract description 59
- 238000007789 sealing Methods 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims description 7
- 238000000605 extraction Methods 0.000 claims description 3
- 235000003140 Panax quinquefolius Nutrition 0.000 claims 1
- 240000005373 Panax quinquefolius Species 0.000 claims 1
- 230000007547 defect Effects 0.000 abstract description 3
- 238000013388 immunohistochemistry analysis Methods 0.000 abstract description 3
- 238000010827 pathological analysis Methods 0.000 abstract description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 12
- 238000010586 diagram Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000010412 perfusion Effects 0.000 description 3
- 230000003902 lesion Effects 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 206010018833 Haematocoele Diseases 0.000 description 1
- 208000005873 Hematocele Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 201000004920 hematocele of tunica vaginalis testis Diseases 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Instructional Devices (AREA)
- Dental Tools And Instruments Or Auxiliary Dental Instruments (AREA)
Abstract
The present invention relates to a kind of lung tissue single channel fixers, it is slow to solve existing lung tissue fixed speed, it influences fixer and submerges efficiency, cause lung tissue exposure duration too long, influence the defect of experimental result, including a fixed ontology, fixed body interior is provided with the accommodating chamber for containing fixer, and the opening of a connection accommodating chamber is provided on fixed ontology, and opening is provided with sealing cover, the wing passage that this body sidewall is provided with a connection accommodating chamber is fixed, is provided with air core pulling in wing passage.As air core pulling removes outward, the volume of accommodating chamber increases, so that the pressure inside accommodating chamber reduces to form negative pressure state, under subnormal ambient, the air inside lung tissue is by rapid deflation, under the elasticity of lung tissue itself, fixer is immersed in alveolar rapidly, alveolar restores to the original state, so that lung tissue cell is fixed complete, convenient for doing immunohistochemistry and pathological analysis below.
Description
Technical field
The present invention relates to a kind of tissue fixation device, especially a kind of lung tissue single channel fixer.
Background technique
When doing animal lung tissue's pathological section, need that lung tissue is fixed, when lung tissue is dyed and is observed, we
It not only needs to observe clearly stained tissue, more requires to keep the intact of pulmonary morphology, avoid disorganization, alveolar collapse
The problems such as.
Currently used lung tissue fixing means mainly has 3 kinds: 1, using embedded box, lung tissue being fixed on formalin
In.2, one small cotton is put in the formalin liquid level for being put into lung tissue, infiltrates lung tissue.3, with perfusion tube by tracheae to
Intrapulmonary injects a small amount of formalin.
Although application method 1 can will cause serious lung in the cracking infiltration to formalin of lung tissue
It withers, destroys the normal organization of lung, and fixer belongs to nature submergence, speed is slower, is easy to influence in experiment
Experimental result.The speed that 2 formalin of method immerses lung tissue is also slow, equally can not achieve the desired results.And method 3 is adopted
With the mode of perfusion, hematocele when perfusion in lung tissue can be adhered to lung surface, influence chipping qualities and observing effect.
Summary of the invention
It is slow that the present invention solves existing lung tissue fixed speed, influences fixer submergence efficiency, causes lung tissue exposure
Overlong time influences the defect of experimental result, provides a kind of lung tissue single channel fixer, air core pulling is arranged in single channel,
Fixer inner air is extracted by air core pulling, accelerates the discharge of lung tissue inner air, while also accelerating fixer and immersing, contracting
Short lung tissue exposure duration, it is ensured that experimental result is correct.
This invention also solves causing tissue seriously miniature when existing lung tissue is fixed, it is easily destroyed the normal of lung tissue
Structure influences the defect of experimental result, provides a kind of lung tissue single channel fixer, and lung tissue is sunk naturally, taken out by air
Core accelerates the discharge of lung tissue inner air, accelerates fixer and immerses, will not destroy lung tissue normal configuration, it is ensured that experimental result is just
Really.
The technical solution adopted by the present invention to solve the technical problems is: a kind of lung tissue single channel fixer, including one
A fixed ontology, fixed body interior are provided with the accommodating chamber for containing fixer, are provided with a connection on fixed ontology and accommodate
The opening of chamber, opening are provided with sealing cover, the wing passage that this body sidewall is provided with a connection accommodating chamber are fixed, in wing passage
It is provided with air core pulling.Fixer, sealing cover closing opening are loaded in accommodating chamber, lung tissue is put into accommodating chamber from opening
Interior, as air core pulling removes outward, the volume of accommodating chamber increases, so that the pressure inside accommodating chamber reduces to form negative pressure shape
State, under subnormal ambient, the air inside lung tissue is by rapid deflation, and under the elasticity of lung tissue itself, fixer soaks rapidly
Entering into alveolar, alveolar restores to the original state, and the position of those lesions loses original function, and morphosis remains unchanged, so that
Lung tissue cell is fixed complete, convenient for doing immunohistochemistry and pathological analysis below.
Preferably, being provided with several positioning elements on fixed ontology, several and positioning element is provided in air core pulling
The fixation member matched, fixation member match the extraction length for limiting air core pulling with corresponding positioning element.
Preferably, air core pulling is in piston-like, including cylindrical piston head and piston rod, piston head are in wing passage
Interior, piston rod reaches outside wing passage.
Preferably, piston head is in the form of annular discs, the periphery of disk is provided with three grooves, and groove has been embedded in sealing ring,
In three seals circle, intermediate for O-ring seal, two sides are rectangular seal.
Preferably, the height of wing passage is equal with the height of accommodating chamber, i.e. air core pulling directly will fixed body interior
It is separated into accommodating chamber and wing passage.
Preferably, the height of wing passage is lower than the height of accommodating chamber, wing passage is in the position of fixed body upper part, Gu
The liquid level of liquid is determined lower than wing passage lower position.
Preferably, the surface of sealing cover towards accommodating chamber is connected with a hollow expander, inside expander filled with
The air of standard pressure.Accommodating chamber removes outward with air core pulling and gradually forms subnormal ambient, expander expansion, and expander has
Have suitable elasticity, the expansion of expander lags behind the emptying of lung tissue air, etc. when fixer immerses after the emptying of lung tissues air,
Expander just expands, and the pressure inside accommodating chamber has slight rebound at this time, this, which gos up, just helps alveolar to restore to the original state.
The beneficial effects of the present invention are: be loaded with fixer in accommodating chamber, sealing cover closing opening, with air core pulling to
It removes outside, the volume of accommodating chamber increases, so that the pressure inside accommodating chamber reduces to form negative pressure state, lung tissue is put from opening
Enter into accommodating chamber, under subnormal ambient, the air inside lung tissue is by rapid deflation, under the elasticity of lung tissue itself, Gu
Determine liquid to be immersed in alveolar rapidly, alveolar restores to the original state, the position of those lesions, loses original function, and morphosis is protected
Hold it is constant so that lung tissue cell is fixed complete, convenient for doing immunohistochemistry and pathological analysis below.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of the present invention；
Fig. 2 is the use schematic diagram of structure shown in Fig. 1 of the present invention；
Fig. 3 is second of structural schematic diagram of the invention；
Fig. 4 is the use schematic diagram of structure shown in Fig. 3 of the present invention；
In figure: 1, fixed ontology, 2, wing passage, 3, air core pulling, 4, fixation member, 5, positioning element, 6, rectangular seal
Circle, 7, O-ring seal, 8, fixer, 9, accommodating chamber, 10, sealing cover.
Specific embodiment
Below by specific embodiment, and in conjunction with attached drawing, the technical solutions of the present invention will be further described.
Embodiment 1: a kind of lung tissue single channel fixer (referring to Fig. 1), including a fixed ontology 1, fixed ontology are in
Cylinder, fixed body interior are provided with the accommodating chamber 9 for containing fixer 8, and fixed body upper part is provided with a connection and accommodates
The opening of chamber, opening are provided with sealing cover 10, fix the wing passage 2 that this body sidewall is provided with a connection accommodating chamber, and side is logical
Air core pulling 3 is provided in road.
The height of wing passage and the height of accommodating chamber are equal, i.e. fixed body interior is directly separated into receiving by air core pulling
Chamber and wing passage.Air core pulling is in piston-like, including cylindrical piston head and piston rod, piston head are in wing passage, piston
Bar reaches outside wing passage.Piston head is in the form of annular discs, and the periphery of disk is provided with three grooves, and groove has been embedded in sealing ring,
In three seals circle, intermediate for O-ring seal 7, two sides are rectangular seal 6.Several positioning are provided on fixed ontology
Component, is provided with several fixation members matched with positioning element in air core pulling, fixation member and corresponding positioning element
Match the extraction length for limiting air core pulling.
Fixer, i.e. 10% formalin are first filled in accommodating chamber, the liquid level of fixer is in the 2/3 of accommodating chamber
Lung tissue is then put by eminence from opening, and horse back close encapsulation lid then removes air core pulling outward, accommodating chamber
Volume increases, so that the pressure inside accommodating chamber reduces to form negative pressure state, the air quilt under subnormal ambient, inside lung tissue
Rapid deflation, under the elasticity of lung tissue itself, fixer is immersed in alveolar rapidly, and alveolar restores to the original state.Fixation member with
Corresponding one of positioning element, which matches, fixes air core pulling, lasts long enough, until lung tissue is soaked completely
Not (referring to fig. 2).
Embodiment 2: a kind of lung tissue single channel fixer (referring to Fig. 3 Fig. 4), difference from Example 1 is: side is logical
The height in road is lower than the height of accommodating chamber, and wing passage is in the position of fixed body upper part, and the liquid level of fixer is lower than wing passage
Lower position.Remaining structure is referring to embodiment 1.
Embodiment 3: a kind of lung tissue single channel fixer, place unlike the embodiments above are: sealing cover direction is held
Receiving the surface of chamber is connected with a hollow expander, the air filled with standard pressure inside expander.The expansion system of expander
Number is suitable with the elastic restitution coefficient of lung tissue, after the expansion of expander betides the emptying of lung tissue air.Remaining structure reference
Embodiment 1 or embodiment 2.
Embodiment described above is several preferred versions of the invention, not makees limit in any form to the present invention
System, there are also other variations and modifications on the premise of not exceeding the technical scheme recorded in the claims.
Claims (4)
1. a kind of lung tissue single channel fixer, it is characterised in that including a fixed ontology, fixed body interior is provided with Sheng
The accommodating chamber of fixer is filled, the opening of a connection accommodating chamber is provided on fixed ontology, opening is provided with sealing cover, fixed
This body sidewall is provided with the wing passage of a connection accommodating chamber, is provided with air core pulling in wing passage；The height of wing passage is lower than
The height of accommodating chamber, wing passage are in the position of fixed body upper part, and the liquid level of fixer is lower than wing passage lower position；Sealing
Lid is connected with a hollow expander, the air filled with standard pressure inside expander, expander towards the surface of accommodating chamber
Expansion lag behind lung tissue air emptying, etc. lung tissues air emptying after fixer immerse when, expander just expands.
2. lung tissue single channel fixer according to claim 1, it is characterised in that it is several fixed to be provided on fixed ontology
Position component, is provided with several fixation members matched with positioning element in air core pulling, fixation member and corresponding positioning region
Part matches the extraction length for limiting air core pulling.
3. lung tissue single channel fixer according to claim 1, it is characterised in that air core pulling is in piston-like, including circle
Cylindrical piston head and piston rod, piston head are in wing passage, and piston rod reaches outside wing passage.
4. lung tissue single channel fixer according to claim 3, it is characterised in that piston head is in the form of annular discs, outside disk
It is provided with three grooves week, groove has been embedded in sealing ring, and in three seals circle, intermediate for O-ring seal, two sides are square
Shape sealing ring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510663538.5A CN105388038B (en) | 2015-10-15 | 2015-10-15 | Lung tissue single channel fixer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510663538.5A CN105388038B (en) | 2015-10-15 | 2015-10-15 | Lung tissue single channel fixer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105388038A CN105388038A (en) | 2016-03-09 |
| CN105388038B true CN105388038B (en) | 2019-01-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510663538.5A Active CN105388038B (en) | 2015-10-15 | 2015-10-15 | Lung tissue single channel fixer |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109292268A (en) * | 2018-12-03 | 2019-02-01 | 兰州大学 | A kind of pathology department sample infuser device and its application method |
| CN111811917B (en) * | 2020-06-11 | 2024-01-02 | 深圳市领先医疗服务有限公司 | Tissue embedding device and system |
| CN113267389B (en) * | 2021-04-29 | 2022-11-15 | 南方医科大学 | Lung tissue specimen fixing device and method |
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|---|---|---|---|---|
| US5089288A (en) * | 1989-06-24 | 1992-02-18 | Berger Hermann J | Method for impregnating tissue samples in paraffin |
| JP2010239907A (en) * | 2009-04-07 | 2010-10-28 | Olympus Corp | Device for transferring adipose tissue and device for separating cell derived from fat |
| JP2012055244A (en) * | 2010-09-09 | 2012-03-22 | Olympus Corp | Apparatus for treating living body tissue |
| CN202362181U (en) * | 2011-11-28 | 2012-08-01 | 田亚宁 | Plastic marrow immunohistochemical embedding box |
| CN202502003U (en) * | 2012-01-31 | 2012-10-24 | 云南省烟草农业科学研究院 | Fresh plant specimen treatment device |
| CN204411479U (en) * | 2015-01-21 | 2015-06-24 | 王全玉 | A kind of Simple negative-pressure device |
| CN204461847U (en) * | 2015-01-29 | 2015-07-08 | 中国医科大学附属第一医院 | The multiple prop of lung tissue sample |
-
2015
- 2015-10-15 CN CN201510663538.5A patent/CN105388038B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5089288A (en) * | 1989-06-24 | 1992-02-18 | Berger Hermann J | Method for impregnating tissue samples in paraffin |
| JP2010239907A (en) * | 2009-04-07 | 2010-10-28 | Olympus Corp | Device for transferring adipose tissue and device for separating cell derived from fat |
| JP2012055244A (en) * | 2010-09-09 | 2012-03-22 | Olympus Corp | Apparatus for treating living body tissue |
| CN202362181U (en) * | 2011-11-28 | 2012-08-01 | 田亚宁 | Plastic marrow immunohistochemical embedding box |
| CN202502003U (en) * | 2012-01-31 | 2012-10-24 | 云南省烟草农业科学研究院 | Fresh plant specimen treatment device |
| CN204411479U (en) * | 2015-01-21 | 2015-06-24 | 王全玉 | A kind of Simple negative-pressure device |
| CN204461847U (en) * | 2015-01-29 | 2015-07-08 | 中国医科大学附属第一医院 | The multiple prop of lung tissue sample |
Non-Patent Citations (1)
| Title |
|---|
| 负压下血管灌流固定兔肺组织的SEM观察;马蓓 等;《内蒙古农牧学院学报》;19920331;第13卷(第1期);第64-67页 |
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|---|---|
| CN105388038A (en) | 2016-03-09 |
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