CN105381039A - Traditional Chinese medicine composition for treating primary osteoporosis and preparation method thereof - Google Patents

Abstract

The invention discloses a traditional Chinese medicine composition for treating primary osteoporosis. The composition is prepared from the following components in parts by mass: 6-15 parts of radix paeoniae rubra, 6-12 parts of moutan bark, 10-15 parts of poria cocos, 3-10 parts of ligusticum wallichii, 3-10 parts of rhizoma corydalis, 3-9 parts of safflower carthamus, 10-15 parts of rhizoma drynariae, 5-15 parts of rhizoma atractylodis macrocephalae, 9-15 parts of teasel, 3-10 parts of pericarpium citri reticulatae, 10-20 parts of ligustrum lucidum ait and 3-10 parts of licorice root. The invention further discloses a preparation method of the traditional Chinese medicine composition for treating primary osteoporosis. The composition can be used for relieving ostealgia, improving symptoms, improving bone density, improving living quality of patients and reducing the incidence rate of side effects, and has high safety, so that the aim of effective treatment of primary osteoporosis and long-time taking can be achieved.

Description

A kind of Chinese medicine composition for the treatment of primary osteoporosis and preparation method thereof

Technical field

The invention belongs to Chinese herbal medicine preparation technology field, be specifically related to a kind of Chinese medicine composition for the treatment of primary osteoporosis, the invention still further relates to a kind of preparation method for the treatment of the Chinese medicine composition of primary osteoporosis.

Background technology

Primary osteoporosis is common senile, degenerative disease, and sickness rate is high, and patient often needs to accept long-term treatment, and this just determines that medicine should have good curative effect, can be suitable for patient's long-term taking again, and safety.Treatment by Chinese herbs osteoporosis, incidence rate clinical symptoms can improved, improve bone density, reduce fragility fractures.Current research and development are treated osteoporotic new drug and be there is following problem: (1) patients with osteoporosis mostly is old people, often with various diseases, and clinical syndrome relative complex.Chinese medicine thinks that osteoporotic morbidity Etiological is suffered from a deficiency of the kidney, the normal syndromes such as folder liver is lost, weakness of the spleen and stomach, blood stasis of holding concurrently, determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs is when considering, and existing new Chinese medicine great majority take invigorating the kidney and strengthening the bones as principle, dialectical comprehensive not, have certain limitation, clinical practice not only clinical efficacy is not good enough, and long-term taking may cause the side effect such as hepatic and renal function injure, do not reach the object of effectively treatment.(2) medicine of new Chinese medicine forms at present is invigorating the liver and kidney mostly, class of invigorating blood circulation medicine, and the overall prescription property of medicine is partially warm, and long-term taking easily causes the side effect of digestive tract aspect, clinical unsuitable long-term taking.

Summary of the invention

The object of this invention is to provide a kind of Chinese medicine composition for the treatment of primary osteoporosis, solve the problem that reasonable not, the overall property of medicine of the prescription existed in prior art is partially warm, long-term prescription may cause the generation of toxic and side effects.

Another object of the present invention is to provide a kind of preparation method for the treatment of the Chinese medicine composition of primary osteoporosis.

First technical scheme of the present invention is, a kind of Chinese medicine composition for the treatment of primary osteoporosis, comprises following component according to mass parts: Radix Paeoniae Rubra 6 parts ~ 15 parts, Cortex Moutan 6 parts ~ 12 parts, 10 parts ~ 15 parts, Poria, Rhizoma Chuanxiong 3 parts ~ 10 parts, Rhizoma Corydalis 3 parts ~ 10 parts, 3 parts ~ 9 parts, Flos Carthami, Rhizoma Drynariae 10 parts ~ 15 parts, the Rhizoma Atractylodis Macrocephalae 5 parts ~ 15 parts, Radix Dipsaci 9 parts ~ 15 parts, Pericarpium Citri Reticulatae 3 parts ~ 10 parts, Fructus Ligustri Lucidi 10 parts ~ 20 parts, 3 parts ~ 10 parts, Radix Glycyrrhizae.

Further, this Chinese medicine composition comprises following component according to mass parts: Radix Paeoniae Rubra 10 parts, Cortex Moutan 10 parts, 12 parts, Poria, Rhizoma Chuanxiong 10 parts, Rhizoma Corydalis 10 parts, 9 parts, Flos Carthami, Rhizoma Drynariae 15 parts, the Rhizoma Atractylodis Macrocephalae 8 parts, Radix Dipsaci 15 parts, Pericarpium Citri Reticulatae 5 parts, Fructus Ligustri Lucidi 15 parts, 3 parts, Radix Glycyrrhizae.

Second technical scheme of the present invention is, a kind of preparation method for the treatment of the Chinese medicine composition of primary osteoporosis, specifically comprises the following steps:

Step 1, weighs, weighs following component according to mass parts: Radix Paeoniae Rubra 6 parts ~ 15 parts, Cortex Moutan 6 parts ~ 12 parts, 10 parts ~ 15 parts, Poria, Rhizoma Chuanxiong 3 parts ~ 10 parts, Rhizoma Corydalis 3 parts ~ 10 parts, 3 parts ~ 9 parts, Flos Carthami, Rhizoma Drynariae 10 parts ~ 15 parts, the Rhizoma Atractylodis Macrocephalae 5 parts ~ 15 parts, Radix Dipsaci 9 parts ~ 15 parts, Pericarpium Citri Reticulatae 3 parts ~ 10 parts, Fructus Ligustri Lucidi 10 parts ~ 20 parts, 3 parts ~ 10 parts, Radix Glycyrrhizae;

Step 2, washes solarization medicine,

All medicine removals of contamination step 1 prepared are cleaned, are dried, by means of sunning sterilization;

Step 3, concocts,

The Rhizoma Drynariae sheet of step 2 of learning from else's experience process, carries out stir-baking with sand, fries to heaving, and takes out, unhairing;

The Rhizoma Corydalis of step 2 of learning from else's experience process, add quantitative rice vinegar, mix thoroughly, moistening exhausts to vinegar, slow fire heating fried dry, and take out, sieve removes chip;

The Radix Dipsaci of step 2 of learning from else's experience process, Poria, the Rhizoma Atractylodis Macrocephalae, Pericarpium Citri Reticulatae, Fructus Ligustri Lucidi, Radix Paeoniae Rubra, Cortex Moutan, Flos Carthami, Rhizoma Chuanxiong, Radix Glycyrrhizae, raw use, does not concoct;

Step 4, decocts,

Medicine mixing is loaded in the container be filled with water by said ratio by said medicine afterwards, by container heating, steaming and decocting, boil 30 minutes, well-done pharmaceutical filtration, medicinal residues are added water 400ml by filtering medicinal residues again, boil 10 minutes, then by twice obtained decoction of medicinal liquid mixing, the Chinese medicine composition for the treatment of primary osteoporosis is.

The invention has the beneficial effects as follows: formula of the present invention is the Empirical formula for the treatment of primary osteoporosis, cure mainly Chinese medical discrimination based on caused by liver and kidney deficiency, have the patients with osteoporosis of weakness of the spleen and stomach and blood stasis syndrome concurrently, with avirulent Chinese prescription, with invigorating the liver and kidney strengthening bone and muscle, strengthening the spleen and stomach, blood-activating and qi-promoting, eliminating stasis to stop pain, clearing away heat and cooling blood is treatment criterion, full side's diagnosis and prescription is reasonable, comprehensively, zoopery proves that we can significantly improve bone density, and illustrate its cure mechanism from molecular mechanism, Clinical practice can alleviate osteodynia, improve symptom, improve bone density, improve the quality of life of patient, reduce the incidence rate of side effect, safety is high, thus reach and effectively can treat primary osteoporosis, again can the object of long-term taking.

Detailed description of the invention

Below in conjunction with detailed description of the invention, the present invention is described in detail.

The invention provides a kind of Chinese medicine composition for the treatment of primary osteoporosis, comprise following component according to mass parts: Radix Paeoniae Rubra 6 parts ~ 15 parts, Cortex Moutan 6 parts ~ 12 parts, 10 parts ~ 15 parts, Poria, Rhizoma Chuanxiong 3 parts ~ 10 parts, Rhizoma Corydalis 3 parts ~ 10 parts, 3 parts ~ 9 parts, Flos Carthami, Rhizoma Drynariae 10 parts ~ 15 parts, the Rhizoma Atractylodis Macrocephalae 5 parts ~ 15 parts, Radix Dipsaci 9 parts ~ 15 parts, Pericarpium Citri Reticulatae 3 parts ~ 10 parts, Fructus Ligustri Lucidi 10 parts ~ 20 parts, 3 parts ~ 10 parts, Radix Glycyrrhizae.

Further, this Chinese medicine composition comprises following component according to mass parts: Radix Paeoniae Rubra 10 parts, Cortex Moutan 10 parts, 12 parts, Poria, Rhizoma Chuanxiong 10 parts, Rhizoma Corydalis 10 parts, 9 parts, Flos Carthami, Rhizoma Drynariae 15 parts, the Rhizoma Atractylodis Macrocephalae 8 parts, Radix Dipsaci 15 parts, Pericarpium Citri Reticulatae 5 parts, Fructus Ligustri Lucidi 15 parts, 3 parts, Radix Glycyrrhizae.

We are with invigorating the liver and kidney strengthening bone and muscle, strengthening the spleen and stomach, blood-activating and qi-promoting, eliminating stasis to stop pain, clearing away heat and cooling blood is the rule for the treatment of, Radix Dipsaci, Rhizoma Drynariae is monarch drug, merit gets its invigorating the liver and kidney, bone and muscle strengthening, the Rhizoma Atractylodis Macrocephalae, Poria, Pericarpium Citri Reticulatae replenishing QI to invigorate the spleen stomach, all is ministerial drug, be equipped with Flos Carthami simultaneously, Rhizoma Chuanxiong, Rhizoma Corydalis promoting blood circulation to remove obstruction in the collateral, promoting the circulation of QI to relieve pain, Radix Paeoniae Rubra, Cortex Moutan clearing away heat and cooling blood, be adjuvant drug altogether with Fructus Ligustri Lucidi enriching yin and nourishing kidney, Radix Glycyrrhizae is for making coordinating the actions of various ingredients in a prescription, all medicines share, Chinese medical discrimination is more comprehensive, solve caused by liver and kidney deficiency double folder weakness of the spleen and stomach clinically, the treatment problem of the complicated syndrome of Osteoporosis of blood stasis syndrome, compatibility clearing away heat and cooling blood medicine simultaneously, reduce the inclined warm nature of overall prescription medicine, be conducive to long-term taking like this, safety is good.By above-mentioned reasonable formula, clinical practice proves that we can significantly improve bone mineral density, alleviates osteodynia, improves General Symptoms, improve the quality of living, take safety, play the object of effectively treating primary osteoporosis.Zoopery proves that our cure mechanism is: by improving the protein expression level of OPG gene, reduce the protein expression level of RNAKL gene, affect OPG-RANKL-RANK system, thus regulate the differentiation of osteoclast, reduce I type Procollagen C propetide (PICP) level, improve serum bone alkaline phosphatase (BALP) level, significantly improve the bone density of osteoporosis model rat; Biomechanics experiment proves: we significantly can strengthen load and the stress of the bone breakaway poing of rat model, improves the sclerotin intensity of rat model.

The present invention also provides a kind of preparation method for the treatment of the Chinese medicine composition of primary osteoporosis, specifically comprises the following steps:

Step 1, weighs, weighs following component according to mass parts: Radix Paeoniae Rubra 6 parts ~ 15 parts, Cortex Moutan 6 parts ~ 12 parts, 10 parts ~ 15 parts, Poria, Rhizoma Chuanxiong 3 parts ~ 10 parts, Rhizoma Corydalis 3 parts ~ 10 parts, 3 parts ~ 9 parts, Flos Carthami, Rhizoma Drynariae 10 parts ~ 15 parts, the Rhizoma Atractylodis Macrocephalae 5 parts ~ 15 parts, Radix Dipsaci 9 parts ~ 15 parts, Pericarpium Citri Reticulatae 3 parts ~ 10 parts, Fructus Ligustri Lucidi 10 parts ~ 20 parts, 3 parts ~ 10 parts, Radix Glycyrrhizae.

Step 2, washes solarization medicine

All medicine removals of contamination step 1 prepared are cleaned, are dried, by means of sunning sterilization.

Step 3, concocts,

The Rhizoma Drynariae sheet of step 2 of learning from else's experience process, carries out stir-baking with sand, fries to heaving, and takes out, unhairing.

The Rhizoma Corydalis of step 2 of learning from else's experience process, add quantitative rice vinegar, mix thoroughly, moistening exhausts to vinegar, and slow fire heating fried dry, take out, sieve removes chip.

The Radix Dipsaci of step 2 of learning from else's experience process, Poria, the Rhizoma Atractylodis Macrocephalae, Pericarpium Citri Reticulatae, Fructus Ligustri Lucidi, Radix Paeoniae Rubra, Cortex Moutan, Flos Carthami, Rhizoma Chuanxiong, Radix Glycyrrhizae, raw use, does not concoct.

Step 4, decocts,

Medicine mixing is loaded in the container be filled with water by said ratio by said medicine afterwards, by container heating, steaming and decocting, boils 30 minutes, well-done pharmaceutical filtration, medicinal residues are added water 400ml by filtering medicinal residues again, boil 10 minutes, then by twice obtained decoction of medicinal liquid mixing, take after the meal at twice.

Under instruction of Chinese Medicine theory, this Chinese medicine composition critical medication is: Radix Dipsaci merit gets invigorating the liver and kidney, strong waist knee joint, and Rhizoma Drynariae merit gets the kidney invigorating bone strengthening effect, and two medicines are same with being monarch drug.Rhizoma Atractylodis Macrocephalae merit gets invigorating the spleen and benefiting QI, and Poria merit gets spleen invigorating eliminating dampness by diuresis, and Pericarpium Citri Reticulatae merit gets regulating qi-flowing for strengthening spleen, and three medicines are ministerial drug.Flos Carthami gets its promoting blood circulation to restore menstrual flow, stasis-dispelling and pain-killing effect, and blood-activating and qi-promoting analgesic efficacy got by Rhizoma Chuanxiong, Rhizoma Corydalis promoting blood circulation to remove blood stasis regulating QI to relieve pain, and three medicines share has promoting blood circulation to remove obstruction in the collateral, promoting the circulation of QI to relieve pain function; Radix Paeoniae Rubra, Cortex Moutan cold nature, have clearing away heat and cooling blood effect, and assistant controls the warm property of above-mentioned many medicines, Fructus Ligustri Lucidi enriching yin and nourishing kidney, and many medicines are adjuvant drug.Radix Glycyrrhizae is for making coordinating the actions of various ingredients in a prescription, and all medicines share, indispensable, plays effect of invigorating the kidney and strengthening the bones, replenishing QI to invigorate the spleen, promoting blood circulation, dredging meridian and relieving pain altogether.

This Chinese medicine composition object space key is: Radix Dipsaci, Rhizoma Drynariae, the Rhizoma Atractylodis Macrocephalae, Poria, Pericarpium Citri Reticulatae, Radix Paeoniae Rubra and Cortex Moutan.Radix Dipsaci, Rhizoma Drynariae merit at invigorating the kidney and strengthening the bones, from osteoporosis main cause--suffering from a deficiency of the kidney starts with carries out treatment, solve root problem; The Rhizoma Atractylodis Macrocephalae, Poria and Pericarpium Citri Reticulatae three medicine then from human body the foundation of acquired constitution--taste are set about, solve because weakness of the spleen and stomach causes body innate deficiency, osteoporotic problem occur; Radix Paeoniae Rubra and Cortex Moutan effect are clearing away heat and cooling blood, reduce the warm nature of overall medicine, avoid because long-term taking Chinese medicine causes the generation of toxic and side effects.

Within the using dosage scope that said medicine consumption all specifies in state-promulgated pharmacopoeia, determine through clinical repeatedly checking, there is drug safety sex chromosome mosaicism in dosage, clinical efficacy is not good enough at least at most, considers economic problems simultaneously, and this Chinese medicine composition object space cost performance is best.

Embodiment 1

Treat a preparation method for the Chinese medicine composition of primary osteoporosis, specifically comprise the following steps:

Step 1, weighs, weighs following component according to mass parts: Radix Paeoniae Rubra 10 parts, Cortex Moutan 10 parts, 12 parts, Poria, Rhizoma Chuanxiong 10 parts, Rhizoma Corydalis 10 parts, 9 parts, Flos Carthami, Rhizoma Drynariae 15 parts, the Rhizoma Atractylodis Macrocephalae 8 parts, Radix Dipsaci 15 parts, Pericarpium Citri Reticulatae 5 parts, Fructus Ligustri Lucidi 15 parts, 3 parts, Radix Glycyrrhizae.

Step 2, washes solarization medicine

All medicine removals of contamination step 1 prepared are cleaned, are dried, by means of sunning sterilization.

Step 3, concocts,

The Rhizoma Drynariae sheet of step 2 of learning from else's experience process, carries out stir-baking with sand, fries to heaving, and takes out, unhairing.

The Rhizoma Corydalis of step 2 of learning from else's experience process, add quantitative rice vinegar, mix thoroughly, moistening exhausts to vinegar, and slow fire heating fried dry, take out, sieve removes chip.

The Radix Dipsaci of step 2 of learning from else's experience process, Poria, the Rhizoma Atractylodis Macrocephalae, Pericarpium Citri Reticulatae, Fructus Ligustri Lucidi, Radix Paeoniae Rubra, Cortex Moutan, Flos Carthami, Rhizoma Chuanxiong, Radix Glycyrrhizae, raw use, does not concoct.

Step 4, decocts,

Medicine mixing is loaded in the container be filled with water by said ratio by said medicine afterwards, by container heating, steaming and decocting, boils 30 minutes, well-done pharmaceutical filtration, medicinal residues are added water 400ml by filtering medicinal residues again, boil 10 minutes, then by twice obtained decoction of medicinal liquid mixing, take after the meal at twice.

Embodiment 2

Treat a preparation method for the Chinese medicine composition of primary osteoporosis, specifically comprise the following steps:

Step 1, weighs, weighs following component according to mass parts: Radix Paeoniae Rubra 6 parts, Cortex Moutan 12 parts, 10 parts, Poria, Rhizoma Chuanxiong 10 parts, Rhizoma Corydalis 3 parts, 9 parts, Flos Carthami, Rhizoma Drynariae 10 parts, the Rhizoma Atractylodis Macrocephalae 15 parts, Radix Dipsaci 9 parts, Pericarpium Citri Reticulatae 10 parts, Fructus Ligustri Lucidi 10 parts, 10 parts, Radix Glycyrrhizae.

Step 2, washes solarization medicine

All medicine removals of contamination step 1 prepared are cleaned, are dried, by means of sunning sterilization.

Step 3, concocts,

The Rhizoma Drynariae sheet of step 2 of learning from else's experience process, carries out stir-baking with sand, fries to heaving, and takes out, unhairing.

The Rhizoma Corydalis of step 2 of learning from else's experience process, add quantitative rice vinegar, mix thoroughly, moistening exhausts to vinegar, and slow fire heating fried dry, take out, sieve removes chip.

The Radix Dipsaci of step 2 of learning from else's experience process, Poria, the Rhizoma Atractylodis Macrocephalae, Pericarpium Citri Reticulatae, Fructus Ligustri Lucidi, Radix Paeoniae Rubra, Cortex Moutan, Flos Carthami, Rhizoma Chuanxiong, Radix Glycyrrhizae, raw use, does not concoct.

Step 4, decocts,

Medicine mixing is loaded in the container be filled with water by said ratio by said medicine afterwards, by container heating, steaming and decocting, boils 30 minutes, well-done pharmaceutical filtration, medicinal residues are added water 400ml by filtering medicinal residues again, boil 10 minutes, then by twice obtained decoction of medicinal liquid mixing, take after the meal at twice.

Embodiment 3

Treat a preparation method for the Chinese medicine composition of primary osteoporosis, specifically comprise the following steps:

Step 1, weighs, weighs following component according to mass parts: Radix Paeoniae Rubra 15 parts, Cortex Moutan 6 parts, 15 parts, Poria, Rhizoma Chuanxiong 3 parts, Rhizoma Corydalis 10 parts, 3 parts, Flos Carthami, Rhizoma Drynariae 15 parts, the Rhizoma Atractylodis Macrocephalae 5 parts, Radix Dipsaci 15 parts, Pericarpium Citri Reticulatae 3 parts, Fructus Ligustri Lucidi 20 parts, 3 parts, Radix Glycyrrhizae.

Step 2, washes solarization medicine

All medicine removals of contamination step 1 prepared are cleaned, are dried, by means of sunning sterilization.

Step 3, concocts,

The Rhizoma Drynariae sheet of step 2 of learning from else's experience process, carries out stir-baking with sand, fries to heaving, and takes out, unhairing.

The Rhizoma Corydalis of step 2 of learning from else's experience process, add quantitative rice vinegar, mix thoroughly, moistening exhausts to vinegar, and slow fire heating fried dry, take out, sieve removes chip.

The Radix Dipsaci of step 2 of learning from else's experience process, Poria, the Rhizoma Atractylodis Macrocephalae, Pericarpium Citri Reticulatae, Fructus Ligustri Lucidi, Radix Paeoniae Rubra, Cortex Moutan, Flos Carthami, Rhizoma Chuanxiong, Radix Glycyrrhizae, raw use, does not concoct.

Step 4, decocts,

Medicine mixing is loaded in the container be filled with water by said ratio by said medicine afterwards, by container heating, steaming and decocting, boils 30 minutes, well-done pharmaceutical filtration, medicinal residues are added water 400ml by filtering medicinal residues again, boil 10 minutes, then by twice obtained decoction of medicinal liquid mixing, take after the meal at twice.

Embodiment 4

Treat a preparation method for the Chinese medicine composition of primary osteoporosis, specifically comprise the following steps:

Step 1, weighs, weighs following component according to mass parts: Radix Paeoniae Rubra 9 parts, Cortex Moutan 8 parts, 14 parts, Poria, Rhizoma Chuanxiong 5 parts, Rhizoma Corydalis 5 parts, 5 parts, Flos Carthami, Rhizoma Drynariae 12 parts, the Rhizoma Atractylodis Macrocephalae 10 parts, Radix Dipsaci 12 parts, Pericarpium Citri Reticulatae 8 parts, Fructus Ligustri Lucidi 12 parts, 8 parts, Radix Glycyrrhizae.

Step 2, washes solarization medicine

All medicine removals of contamination step 1 prepared are cleaned, are dried, by means of sunning sterilization.

Step 3, concocts,

The Rhizoma Drynariae sheet of step 2 of learning from else's experience process, carries out stir-baking with sand, fries to heaving, and takes out, unhairing.

The Rhizoma Corydalis of step 2 of learning from else's experience process, add quantitative rice vinegar, mix thoroughly, moistening exhausts to vinegar, and slow fire heating fried dry, take out, sieve removes chip.

The Radix Dipsaci of step 2 of learning from else's experience process, Poria, the Rhizoma Atractylodis Macrocephalae, Pericarpium Citri Reticulatae, Fructus Ligustri Lucidi, Radix Paeoniae Rubra, Cortex Moutan, Flos Carthami, Rhizoma Chuanxiong, Radix Glycyrrhizae, raw use, does not concoct.

Step 4, decocts,

Medicine mixing is loaded in the container be filled with water by said ratio by said medicine afterwards, by container heating, steaming and decocting, boils 30 minutes, well-done pharmaceutical filtration, medicinal residues are added water 400ml by filtering medicinal residues again, boil 10 minutes, then by twice obtained decoction of medicinal liquid mixing, take after the meal at twice.

Clinical case of the present invention:

Patient Lin, female, 72 years old, aching pain in waist and back recurrent exerbation 2 years, with tinnitus, night, lower limb knotted, the soft knee joint acid of shin, occasionally there is anorexia, defecate often rare soft, fatiguability after tired, check: ordinary circumstance can, lumbar vertebra is without obvious tenderness, joint motion is normal, light red tongue, thin fur, tooth is had to print, stringy and thready pulse delays, lumbar spine bmd detects prompting osteoporosis, treatment gives this Chinese medicine composition, every day 1 dose, take after the meal at twice, treatment two weeks, aching pain in waist and back obviously alleviates, lower limb cramp number of times reduces, stool can be shaped, treat 3 months, soreness of waist and knee joint is alleviated, night knots without lower limb, appetite is normal, stool is normal, tinnitus obviously alleviates, without other malaise symptoms.Continue to take medicine 3 months, health is without malaise symptoms, and normally, lumbar spine bmd significantly improves before detecting comparatively treatment, and period in a medicine reacts without discomfort for diet, stool.

Chen, female, 68 years old, soreness of waist and knee joint 1 year, night occasionally has lower limb to knot, and appetite is not good enough, and stool is even rare soft, walking half an hour after soreness of the waist and knees weak, check: ordinary circumstance can, waist knee joint is without tenderness, and joint motion is normal, light red tongue, white and thin fur, tooth is had to print, stringy and thready pulse, lumbar spine bmd detects prompting osteoporosis, and treatment gives this Chinese medicine composition, every day 1 dose, take after the meal at twice, treatment two weeks, soreness of waist and knee joint, lower limb cramp symptom are obviously improved, treat 3 months, uncomfortable symptom is alleviated substantially.Continue to take medicine 3 months, health is without discomfort, and lumbar spine bmd significantly improves before detecting comparatively treatment.Period in a medicine has no side effect generation.

Poplar, female, 62 years old, 3 months Shen Ti Of-thin were weak for aching pain in waist and back, and night, lower limb knotted, walking for a long time fatiguability, anorexia, loose stool, check that lumbar vertebra is without tenderness, joint motion is normal, and light red tongue is fat, white and thin fur, have tooth to print, stringy pulse, lumbar spine bmd detects prompting osteoporosis, and treatment gives this Chinese medicine composition, every day 1 dose, take after the meal at twice, treatment two weeks, the cramp of soreness of waist and knee joint, lower limb, appetite, loose stool symptom are obviously improved, and treat 3 months, uncomfortable symptom is alleviated substantially.Continue to take medicine 3 months, health is without malaise symptoms, and lumbar spine bmd significantly improves before detecting comparatively treatment.Have no side effect during medication generation.

Beneficial effect of the present invention is discussed below in conjunction with concrete experimentation:

One, material

1. laboratory animal

6 monthly age cleaning grade female sd inbred rats 40, are provided by Shanghai Slac Experimental Animal Co., Ltd., laboratory animal credit number: SCXK (Shanghai) 2007-0005, and the quality certification is numbered: 2007000530078.Raise in Fujian Institute of Traditional Chinese Medicine's comparative medicine experimental center, medical experiment animal environment facility is cleaning grade [quality certification number: SYXK (Fujian) 2009-000].

2. Experimental agents

The present composition forms: crude drug is purchased by pharmaceuticals of Fujian Province.

Rat oral gavage amount computational methods:

People 50Kg:122 ÷ 50=2.44g/kg/ days

Rat oral gavage amount: 2.44 × 6.25 times=15g/kg

122g÷81.33ml＝1.5g/ml

After potion traditional Chinese medicine decocting, standardize solution is to 81.33ml, and crude drug content is 1.5g/ml.

Namely rat oral gavage amount is 1ml/100g rat body weight.

By Chinese medicine tradition decocting method, 10 doses of formula prepared slices of Chinese crude drugs 1220g adding distil waters of the present invention are soaked 30min, boil 30min, incline and medicinal liquid, medicinal residues add 5 times of distilled water, then boil 20min, incline and medicinal liquid, mix 2 medicinal liquids and filter, be concentrated into 813.0ml (1.5g crude drug/ml), subpackage, for subsequent use in 4 DEG C of Refrigerator stores.

3. reagent

Allan sodium phosphate sheet (Fosamax) Hangzhou Mo Shadong pharmaceutical Co. Ltd produces, specification: 70mg/ sheet; Gaierqi D (vitamin D3 and calcium carbonate) Wyeth pharmaceuticals produces, specification: 600mg/ sheet; Elisa test kit (TSZ company of the U.S.), Elisa test kit (TSZ company of the U.S.), pvdf membrane (Invitrogen company); Primary antibodie (Abcam company); ECL luminescence reagent (ThermoScientific company).

4. instrument

DiscoveryWi Dual-energy X-rays absorptionmetry (Hologic company of the U.S.), universal testing machine AGIC-20KN (Japanese Shimadzu Corporation), multicolor fluorescence/chemiluminescence imaging work station (ProteinsimpleFluorChemM company of the U.S.), detection of nucleic acids instrument 2100 (Agilent company of the U.S.).

Two, method

1. divide into groups

Select the healthy 6 monthly age SD female rats 40 of cleaning grade, be divided into 4 groups at random: Gaierqi D (vitamin D3 and calcium carbonate) group, formula group of the present invention, sham operated rats, removal ovary osteoporosis model group.Often organize 10.

2, the modeling of osteoporosis model rat and administration

Select SD rat 40, the capable bilateral oophorectomy of modeling method, concrete steps are as follows: with 2% pentobarbital sodium 40mg/kg intraperitoneal injection of anesthesia, operative site cropping is sterilized, and gets abdominal part median incision and is about 2cm, after entering abdominal cavity, the uterus of visible rat Y shape, visit and left and right end ovary, after row ligation excision, by stomach wall layer-by-layer suture along uterus bilateral.Postoperative 3 days continuous intramuscular injection penicillin prevention infection (80,000 units/only).Sham operated rats is except non-row ovary ligation excision, and all the other steps are identical with operation group.Started gavage treatment in postoperative 30 days, rat dosage is etc. 6.25 times of the clinical dose of body weight.

Specific as follows: Alendronate sodium group gavage amount is 8.2mg/kg, once in a week; Gaierqi D (vitamin D3 and calcium carbonate) dosage is 0.126g/ (kgd), afternoon gavage; Formula 10ml/kg of the present invention, the morning gavage; Sham operated rats and model group often only give normal saline 10ml/kg.Each treated animal is all at temperature 25C, and relative humidity 70-80%, under the environment of ventilation, illumination abundance, gives generic word material word and support, freely drink water, freely movable.

3. draw materials

Administration is drawn materials after 12 weeks respectively, often organizes 10.After 10% chloral hydrate anesthesia, by abdominal aortic blood, get bilateral tibial and lumbar vertebra subsequently.After whole blood room temperature of getting leaves standstill Ih, through the centrifugal lOmin of 3000rmp, get serum ,-80 DEG C of preservations.Right side tibia is fixed with 4% neutral paraformaldehyde, and left tibias, with normal saline gauze parcel, is stored in-20 DEG C, for detecting bone density and biomechanics after sealed bag sealing.Lumbar vertebra is directly frozen in-80 DEG C of refrigerators.

4, detection method

4.1 Bone mineral density

Dual-energy X-rays absorptionmetry is adopted to carry out left tibias bone densitometry.

4.2 bone biomechanicals detect

All left tibias are returned to room temperature after taking out from-20 DEG C of cryogenic refrigerators, and keep normal saline moistening.After rewarming, adopt three-point bending method, be placed in by tibia on universal testing machine, bearing span 20mm, with tibia mid point for adding force, pressure-loaded speed 1mm/min, until fracture of tibia, its load and distortion trace load-deflection curve by computer.Measure tibia internal diameter, external diameter, draw biomechanical by computer recording analysis: maximum load and maximum stress.

4.3 serum BALP and PICP detect

Adopt Elisa test kit, get enzyme bag by plate, be fixed on framework, standard sample wells, instrument connection and blank control wells are set respectively, in gauge orifice, add standard substance 50ul; After adding 40ul sample diluting liquid in instrument connection, then add 10ul rat blood serum sample; Blank well is application of sample not.After application of sample, with the rearmounted 37 DEG C of incubations of shrouding film shrouding, take shrouding film after 30 minutes off, discard liquid, dry.Concentrated cleaning solution after dilution 20 times is washed plate 5 times, pats dry.Then every hole adds enzyme marking reagent 50 μ l, except blank well.Continue 37 DEG C of incubation 30min, wash plate 5 times.Every hole first adds developer A50 μ l afterwards, then adds developer B50 μ l, and 37 DEG C of lucifuges develop the color 15 minutes.Add stop buffer 50ul cessation reaction (now blue vertical turn of yellow), the absorbance in the last each hole of 450nm wavelength measurement.

4.4OPG, RNAKL protein expression detects

Gross protein is extracted, ultraviolet spectrophotometry photometric determination protein concentration, polyacrylamide gel electrophoresis from lumbar vertebra histiocyte.Turned in liquid at electricity by glue after electrophoresis and soak 15min, the pvdf membrane that clip is equal with gel area and two same electricity that are soaked in of filter paper turn 10min in liquid.Soaked into by porous gasket after electricity turns liquid, install electric rotary device, order is: anode (redness), porous gasket, filter paper, pvdf membrane, gel, filter paper, porous gasket, negative electrode (black).Electric carryover sequence is set: 110v1.5h.Afterwards pvdf membrane is placed in confining liquid, room temperature jog 2h or 4 DEG C spends the night.Wash film with TBST solution, add primary antibodie, two resist, and wash film.Finally adopt super quick ECL chemical luminescence reagent kit and use gel imaging system testing result.

5, statistics

Adopt SPSS17.0 software to carry out data analysis, adopt t inspection to compare, P < 0.05 indicates statistical significance.

Three, result

1. between group, bone density compares

Formula group bone density of the present invention is the highest in 4 groups, bone density is all apparently higher than sham operated rats, Gaierqi D (vitamin D3 and calcium carbonate) group and model group, significant difference very significantly (compares t=2.4867 with Gaierqi D (vitamin D3 and calcium carbonate) group, p < 0.023, t=3.3499 is compared with sham operated rats, p < 0.01, compares t=5.5133 with model group, p < 0.000).In table 1.

Table 1, each group bone density value compare ( g/cm ²)

Note: compare with formula group of the present invention, " * " represents p < 0.05, " * * * " represents p < 0.000.

2, between group, bone biomechanical compares

Formula group breakaway poing maximum load of the present invention is significantly higher than model group (t=2.4469, p < 0.05);

Formula group breakaway poing maximum stress of the present invention is significantly higher than model group (t=2.3521, p < 0.05).In table 2.

Table 2, each group rat breakaway poing load, stress and strain ratio are comparatively

Note: compare with formula group of the present invention, " * " represents p < 0.05.

3, between group, serum BALP and PICP compares

Each group of rat type I Procollagen C propetide (PICP), serum bone alkaline phosphatase (BALP) concentration compare, and formula group BALP concentration of the present invention is apparently higher than model group (t=3.6435, p < 0.01);

Formula group PICP concentration of the present invention is starkly lower than model group (t=4.0491, p < 0.001).In table 3.

Table 3, each group serum BALP, PICP level compare

Note: compare with formula group of the present invention, " * * " represents p < 0.01, and " * * * " represents p < 0.001.4, between group, OPG, RNAKL protein expression compares

Formula group OPG protein concentration of the present invention is apparently higher than model group (t=2.1359, p < 0.05);

Formula group RNAKL protein concentration of the present invention is starkly lower than model group (t=3.2168, p < 0.01).In table 4.

Table 4, respectively organize OPG, RNAKL protein expression level and compare

Note: compare with formula group of the present invention, " * " represents p < 0.05, " * * " represents p < 0.01.

Conclusion

Above-mentioned zoopery proves: formula of the present invention can improve the protein expression level of OPG gene, reduce the protein expression level of RNAKL gene, reduce I type Procollagen C propetide (PICP) level, improve serum bone alkaline phosphatase (BALP) level, significantly improve the bone density of osteoporosis model rat, also significantly strengthen maximum load and the maximum stress of the bone breakaway poing of rat model simultaneously, improve the intensity of bone.

Claims (3)

1. treat a Chinese medicine composition for primary osteoporosis, it is characterized in that, comprise following component according to mass parts: Radix Paeoniae Rubra 6 parts ~ 15 parts, Cortex Moutan 6 parts ~ 12 parts, 10 parts ~ 15 parts, Poria, Rhizoma Chuanxiong 3 parts ~ 10 parts, Rhizoma Corydalis 3 parts ~ 10 parts, 3 parts ~ 9 parts, Flos Carthami, Rhizoma Drynariae 10 parts ~ 15 parts, the Rhizoma Atractylodis Macrocephalae 5 parts ~ 15 parts, Radix Dipsaci 9 parts ~ 15 parts, Pericarpium Citri Reticulatae 3 parts ~ 10 parts, Fructus Ligustri Lucidi 10 parts ~ 20 parts, 3 parts ~ 10 parts, Radix Glycyrrhizae.

2. the Chinese medicine composition for the treatment of primary osteoporosis according to claim 1, it is characterized in that, further, this Chinese medicine composition comprises following component according to mass parts: Radix Paeoniae Rubra 10 parts, Cortex Moutan 10 parts, 12 parts, Poria, Rhizoma Chuanxiong 10 parts, Rhizoma Corydalis 10 parts, 9 parts, Flos Carthami, Rhizoma Drynariae 15 parts, the Rhizoma Atractylodis Macrocephalae 8 parts, Radix Dipsaci 15 parts, Pericarpium Citri Reticulatae 5 parts, Fructus Ligustri Lucidi 15 parts, 3 parts, Radix Glycyrrhizae.

3. treat a preparation method for the Chinese medicine composition of primary osteoporosis, it is characterized in that, specifically comprise the following steps:

Step 1, weighs, weighs following component according to mass parts: Radix Paeoniae Rubra 6 parts ~ 15 parts, Cortex Moutan 6 parts ~ 12 parts, 10 parts ~ 15 parts, Poria, Rhizoma Chuanxiong 3 parts ~ 10 parts, Rhizoma Corydalis 3 parts ~ 10 parts, 3 parts ~ 9 parts, Flos Carthami, Rhizoma Drynariae 10 parts ~ 15 parts, the Rhizoma Atractylodis Macrocephalae 5 parts ~ 15 parts, Radix Dipsaci 9 parts ~ 15 parts, Pericarpium Citri Reticulatae 3 parts ~ 10 parts, Fructus Ligustri Lucidi 10 parts ~ 20 parts, 3 parts ~ 10 parts, Radix Glycyrrhizae;

Step 2, washes solarization medicine,

All medicine removals of contamination step 1 prepared are cleaned, are dried, by means of sunning sterilization;

Step 3, concocts,

The Rhizoma Drynariae sheet of step 2 of learning from else's experience process, carries out stir-baking with sand, fries to heaving, and takes out, unhairing;

The Rhizoma Corydalis of step 2 of learning from else's experience process, add quantitative rice vinegar, mix thoroughly, moistening exhausts to vinegar, slow fire heating fried dry, and take out, sieve removes chip;

The Radix Dipsaci of step 2 of learning from else's experience process, Poria, the Rhizoma Atractylodis Macrocephalae, Pericarpium Citri Reticulatae, Fructus Ligustri Lucidi, Radix Paeoniae Rubra, Cortex Moutan, Flos Carthami, Rhizoma Chuanxiong, Radix Glycyrrhizae, raw use, does not concoct;

Step 4, decocts,

Medicine mixing is loaded in the container be filled with water by said ratio by said medicine afterwards, by container heating, steaming and decocting, boil 30 minutes, well-done pharmaceutical filtration, medicinal residues are added water 400ml by filtering medicinal residues again, boil 10 minutes, then by twice obtained decoction of medicinal liquid mixing, the Chinese medicine composition for the treatment of primary osteoporosis is.