CN105371780B - Optical 3-dimensional relevant identification devices and recognition methods based on integrated imaging system - Google Patents
Optical 3-dimensional relevant identification devices and recognition methods based on integrated imaging system Download PDFInfo
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Abstract
The invention discloses a kind of optical 3-dimensional relevant identification devices based on integrated imaging system and recognition methods, the device includes three-dimensional information acquisition device and Classical correlation system, and three-dimensional information acquisition device includes sample rotating device, light microscope, diffusing transmission element, microlens array, imaging len, computer；Classical correlation system includes laser, the first reflective mirror, the second reflective mirror, upside-down mounting telescopic system, spatial light modulator, first Fourier's transmissive mirror, the 3rd reflective mirror, diaphragm, photorefractive crystal, the second fourier lense, the second ccd sensor and spatial light modulator.Amplifying power of the present invention is good, and image quality is high, and the three-dimensional information of acquisition is comprehensive, and recognition effect is good.
Description
Technical field
The present invention relates to a kind of optical 3-dimensional identification device, more particularly to a kind of small items based on integration imaging are (long
Wide height is less than 2mm × 2mm × 0.1mm) micro- and optical 3-dimensional Classical correlation the device of three-dimensional, Classical correlation implementation method.
Background technology
Optical coherence interferometry method has the advantages that speed is fast, accuracy is high, non-contact, parallel processing and translation invariance
So that it is widely used in object identification field.Wherein, coherent source is not required in integrated imaging method so that and its is simple in structure,
Antijamming capability is stronger, can solve the problems, such as the identification that optical system is difficult to realize three-dimensional body, will have more realistic meaning
And practical value.For example, in medical domain, can Accurate Diagnosis disease to histotomy automatic identification using integrated imaging system；
In microbiological art, integrated imaging system can obtain the image in biological microtissue different depth face, further Three-dimensional Gravity
Structure and Classical correlation can be classified to microorganism and morphological research；In field of food safety, by being identified to target microorganism
Monitoring can assess security status of food；In ecological monitoring field, related microorganisms flora in ecological environment can be monitored by identifying
Change.Therefore the small items optical 3-dimensional relevant identification devices based on integrated imaging system suffer from extensively in numerous areas
Using market application foreground is very considerable, it has also become hot research topic both domestic and external.
At present, the relevant report of integration imaging microscope equipment and recognition methods is all related to both at home and abroad.Such as Nankai University
Reported within 2012 based on camera array three-dimensionally integrated record by imaging system (burnt slight snow, Zhao Xing, " three based on camera array
Tie up integration imaging record system ", optical precision engineering, 2012 (20):1653-1660) realized by camera array three-dimensionally integrated
Imaging, this scheme is not only complicated, but also cannot carry out three-dimensional imaging to small items.Shown for small items integration imaging
Microdevice and recognition methods, have used one to be simply integrated imaging microscope equipment (Javidi B, Moon I, Yeom in the world
S.Three-dimensional identification of biological microorganism using integral
imaging.Opt.Express 2006,14:12096-12108.) realize.The device using amplifying lens to biological tissue into
Row amplification, microlens array is added behind amplifying lens, to record the cell picture array of intensified image, realizes biological specimen
Three-dimensional values and identification.This method is smaller to the enlargement ratio of sample, and directly causes depth direction using amplifying lens
Magnifying power it is uneven, deviation is produced to the record of sample three-dimensional information, and simple lens can produce serious aberration.In the world
Combine also by integration imaging with confocal laser scanning microscopy realizes the three of biological tissue after should being marked to sample
Dimension reconstruct (Jang JS, Javidi B.Three-dimensional integral imaging of micro-
objects.Opt.Lett.2004；29:1230-1232.).This method needs to carry out fluorescent marker to sample, can only be passively
The acquisition of three-dimensional information is carried out, the advantage of the i.e. recordable object dimensional information of integration imaging single imaging, Wu Fajin can not be embodied
Row real-time three-dimensional information processing.Above-mentioned various devices there are amplifying power is relatively low, image quality is poor, obtain three-dimensional information compared with
Less, the defects of sample fine feature can not be obtained.
The content of the invention
For above-mentioned problems of the prior art, it is an object of the present invention to provide a kind of enlargement ratio height, information
Amount to obtain is big, it is compact-sized, small items three-dimensional information can be achieved obtain and the device of optical 3-dimensional Classical correlation and small
Object (length, width and height are less than 2mm × 2mm × 0.1mm) three-dimensional relative identifying method.
In order to realize above-mentioned task, the present invention uses following technical scheme：
A kind of optical 3-dimensional relevant identification devices based on integrated imaging system, including three-dimensional information acquisition device and correlation
Identifying system：
Three-dimensional information acquisition device is included on acquisition of information optical path direction spaced sample rotating device, light successively
Microscope, diffusing transmission element, microlens array and imaging len are learned, object sample is detachably provided with sample rotating device
This；Light passes through the object sample on sample rotating device along acquisition of information light path, then passes through light microscope successively, overflows
Transmissive element, microlens array, imaging len simultaneously pass information to a computer after the conversion of the first ccd sensor；
Classical correlation system includes laser, and the light that laser is sent is divided into reference path and object light road by spectroscope,
The first reflective mirror, the second reflective mirror, upside-down mounting telescopic system, spatial light modulator and are sequentially arranged at intervals with object light road
One Fourier's transmissive mirror；The 3rd reflective mirror and diaphragm are sequentially arranged at intervals with reference path, in reference path and object light road
Light converges on a photorefractive crystal jointly, converges light after photorefractive crystal, then by the second fourier lense
Expose on the second ccd sensor, the computer is passed information to after being changed via the second ccd sensor, it is described
Spatial light modulator is connected with computer.
Further, the object sample is small items sample, and object sample is translucent sample, object sample
Thickness be 40 μm~60 μm；Here translucent refers to that light can be to pass through sample and after body surface is injected through multiple
Scattering absorbs and then forms the optical effect of comparison of light and shade.
Further, the spatial light modulator and photorefractive crystal are located at the front focal plane of the first fourier lense respectively
On back focal plane.
Further, the sample rotating device includes a fixed station for being used for fixed object sample, and fixed station can
Two-dimensional movement is done in the horizontal plane, to realize object sample friction free rotation and translation；The anglec of rotation of the fixed station
It is -180 °~180 ° to spend scope, and rotation resolution ratio is 0.01 °, and translating stroke is ± 100mm, 1 μm of translation resolution.
Further, the lighting source of the light microscope is uniform source of light, its object lens enlargement ratio is 10~60
Times, numerical aperture is 0.3~0.7, and eyepiece enlargement ratio is 10 times.
Further, it is three-dimensional to be used for the extraction carrying object sample from light microscope emergent light for the diffusing transmission element
The scattering light of information is simultaneously projected on microlens array；Diffusing transmission element is by along interval is set successively on microlens array direction
Condenser lens, adjustable zoom lens and the unrestrained projection screen composition put, wherein the focal length of adjustable zoom lens is 12mm~23mm, overflow projection
The thickness of screen is 2mm, and granularity is 400~800mm, and the focal length of condenser lens is 100mm.
Further, the size of the microlens array is 60mm × 50mm, by the autohemagglutination that multiple focal lengths are 1mm
Focus lens form, a diameter of 1mm of GRIN Lens, and the spacing between GRIN Lens is 1mm.
Further, first ccd sensor uses EMCCD, and the pixel number of EMCCD is more than 512 × 512, pixel
Size is less than 20 μm of 20 μ m, and frame speed is more than 33fps.
Further, in the Classical correlation system, laser uses the solid state laser of 532nm, 100mW, laser
The emergent light of device is adjusted to less than 20mW through attenuator.
Further, in the Classical correlation system, via the separated object light road of spectroscope, the splitting ratio of reference path
For 6:4@532nm, are coated with high-reflecting film on the first reflective mirror, the second reflective mirror and the 3rd reflective mirror, the reflectivity of high-reflecting film is big
In 99.8%@532nm；First Fourier's transmissive mirror, the focal length selection range of second Fourier's transmissive mirror for 80mm~
100mm。
Further, the photorefractive crystal is 10mm × 10mm × 10mm for size, mixes iron 0.03wt%, mixes zinc
The LiNbO of 0.6wt%3:Fe crystal.
Further, second ccd sensor is using the high resolution CCD camera for carrying zoom lens, its zoom
Lens focus scope is 12~36mm, and pixel number is more than 1024 × 1024, and pixel dimension is less than 5.2 μm of 5.2 μ m.
A kind of method that optical 3-dimensional Classical correlation is carried out using above device, is comprised the following steps：
Step 1, gets out Reference sample and target object sample as the object sample in processing procedure, and
Reference sample, target object sample are performed Step 2: the processing procedure of step 3, then performs step 4 respectively；
Step 2, places uniform source of light in acquisition of information light path, and the light that uniform source of light is sent, which is radiated at, is placed in sample
Object sample on this rotating device, carries out friction free rotation and translation, through object using sample rotating device to sample
The light of sample is exaggerated by light microscope, to collect the three-dimensional information that transmitted light is included with scattering light；Optical microphotograph
The emergent light of mirror is exported using interface arrangement, reaches diffusing transmission element, obtains the three-dimensional information of sample；Light is via unrestrained saturating
The unrestrained projection light penetrated after element is received by microlens array, and unit is carried out using the first ccd sensor after imaged lens focus
Array image gathers, and will gather information record into computer；
Step 3, the laser beam that laser is sent are divided into two-beam line through spectroscope, and the reflected beams on spectroscope are through
It is used as after three mirror reflections and refers to light beam, the projecting beam on spectroscope changes through the first reflective mirror, the second mirror reflection
Behind direction, expanded via upside-down mounting telescopic system and collimation processing, the homogenizer after processing project space light modulation
On device；
Step 4, the cell array with Reference sample three-dimensional information that computer gathers the first ccd sensor
Image is input in spatial light modulator, Fourier transformation is carried out through the first fourier lense, after the first fourier lense
The frequency spectrum of Reference image is obtained on focal plane, and is interfered with reference beam in photorefractive crystal, so that in Preset grating
Write-in volume hologram is as matched filtering correlator in crystal；
Step 5, the cell array with target object sample three-dimensional information that computer gathers the first ccd sensor
Image is input in spatial light modulator, makes reading of the transmitted light of carrying target object three-dimensional information as matched filtering correlator
Light extraction, carries out inverse Fourier transform by the second fourier lense, reference substance is obtained on the back focal plane of the second fourier lense
The correlation output of body sample and target object sample, is recorded and is input in computer by the second ccd sensor and analyzed, real
Now to the identification of target object.
The present invention has following technical characterstic compared with prior art：
1. the present apparatus uses light microscope, as amplification system, compared to other existing devices, which does not destroy aobvious
Micro-mirror structure, therefore greatly improved for the amplifying power of small items, aberration reduces, and intensified image can more accurately reflect
The characteristic information of sample so that the trickleer characteristic information of sample can be acquired and record；
2. the present apparatus has used the light diffusing transmission element of designed, designed so that microlens array can be to carrying abundant sample
The scattering light of three-dimensional feature information is received, and then is obtained and recorded by unit pattern matrix, this be existing other systems without
What method was completed, such design can not only obtain whole sample three-dimensional informations that existed system can obtain, but also can be with
Obtain more three-dimensional informations；
3. compared with other existing devices, each cell picture that the present apparatus is obtained by microlens array is to sample
The record of Global Information, it is such to design the Viewing-angle information for alloing cell picture array to obtain more samples；
4. compared with other existing recognition methods, integration imaging three-dimensional microscope equipment and optical correlation are known in this recognition methods
Other method is combined, and realizes the optical 3-dimensional Classical correlation of small items.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the present invention；
Fig. 2 is Reference cell picture array in test simulation；
Fig. 3 is target object cell picture array in test simulation；
Fig. 4 is the normalized intensity distribution curve of auto-correlation output；
Fig. 5 is the normalized intensity distribution curve of cross-correlation output.
Figure label represents：1-object sample, 2-sample rotating device, 3-light microscope, 4-diffusing transmission element,
5-microlens array, 6-imaging len, the 7-the first ccd sensor, 8-computer, 9-laser, 10-spectroscope,
11-the first reflective mirror, the 12-the second reflective mirror, the 13-the first lens, the 14-the second lens, 15-spatial light modulator, 16-
First fourier lense, the 17-the three reflective mirror, 18-diaphragm, 19-photorefractive crystal, the 20-the second fourier lense, 21-
Second ccd sensor.
Embodiment
Present disclosure is described in further detail below in conjunction with the accompanying drawings.
With reference to shown in Fig. 1, present invention firstly provides a kind of optical 3-dimensional Classical correlation dress based on integrated imaging system
Put, which includes three-dimensional information acquisition device and Classical correlation system：
Three-dimensional information acquisition device be included on acquisition of information optical path direction successively spaced sample rotating device 2,
Light microscope 3, diffusing transmission element 4, microlens array 5 and imaging len 6, are removably disposed on sample rotating device 2
There is object sample 1；Light passes through the object sample 1 on sample rotating device 2 along acquisition of information light path, then passes through light successively
Learn microscope 3, diffusing transmission element 4, microlens array 5, imaging len 6 and pass information after the conversion of the first ccd sensor 7
Pass a computer 8；
Classical correlation system includes laser 9, and the light that laser 9 is sent is divided for reference path and object light by spectroscope 10
Road, is sequentially arranged at intervals with the first reflective mirror 11, the second reflective mirror 12, upside-down mounting telescopic system, spatial light tune on object light road
15 and first fourier lense 16 of device processed；The 3rd reflective mirror 17 and diaphragm 18, reference light are sequentially arranged at intervals with reference path
Light on road and object light road converges on a photorefractive crystal 19 jointly, converges light after photorefractive crystal 19, then
Expose on the second ccd sensor 21 by the second fourier lense 20, pass information after being changed via the second ccd sensor 21
The computer 8 is passed, the spatial light modulator 15 is connected with computer 8.
The three-dimensional Classical correlation to small items can be achieved in the present invention, thus the object sample 1 in this programme is using small
Object picture sheet.Object sample 1 is translucent sample, and the thickness of object sample 1 is 40 μm~60 μm.
Sample rotating device 2 includes a fixed station for being used for fixed object sample 1, and fixed station can do two in the horizontal plane
Dimension movement, to realize 1 friction free rotation and translation of object sample；The rotation angle range of the fixed station is -180 °
~180 °, rotation resolution ratio is 0.01 °, and translating stroke is ± 100mm, 1 μm of translation resolution；The movement of fixed station is by stepping electricity
Machine is controlled.
The lighting source of light microscope 3 is uniform source of light, its object lens enlargement ratio is 10~60 times, and numerical aperture is
0.3~0.7, eyepiece enlargement ratio is 10 times.
Diffusing transmission element 4 is used for the scattering light that the extraction from 3 emergent light of light microscope carries 1 three-dimensional information of object sample
And project on microlens array 5；Diffusing transmission element 4 is by along close to spaced focusing successively on 5 direction of microlens array
Lens, adjustable zoom lens and unrestrained projection screen composition, wherein the focal length of adjustable zoom lens is 12mm~23mm, overflow the thickness of projection screen
For 2mm, granularity is 400~800mm, and the focal length of condenser lens is 100mm.
Microlens array 5 is placed in after diffusing transmission element 4, its size is 60mm × 50mm, is 1mm by multiple focal lengths
GRIN Lens composition, a diameter of 1mm of GRIN Lens, the spacing between GRIN Lens is 1mm.
Upside-down mounting telescopic system is made of the first lens 13 and the second lens 14.
First ccd sensor 7 uses EMCCD, and the pixel number of EMCCD is more than 512 × 512, and pixel dimension is less than 20 μ ms
20 μm, frame speed is more than 33fps.Laser 9 is using the solid state laser 9 of 532nm, 100mW, and the emergent light of laser 9 is through decay
Device is adjusted to less than 20mW.
In Classical correlation system, the splitting ratio via the separated object light road of spectroscope 10, reference path is 6:4@
532nm, is coated with high-reflecting film on the first reflective mirror 11, the second reflective mirror 12 and the 3rd reflective mirror 17, the reflectivity of high-reflecting film is big
In 99.8%@532nm；First fourier lense 16, the focal length selection range of second Fourier's transmissive mirror for 80mm~
100mm。
Photorefractive crystal 19 is 10mm × 10mm × 10mm for size, mixes iron 0.03wt%, mixes zinc 0.6wt%'s
LiNbO3:Fe crystal.
Second ccd sensor 21 uses the high resolution CCD camera for carrying zoom lens, its zoom lens focal range is
12~36mm, pixel number are more than 1024 × 1024, and pixel dimension is less than 5.2 μm of 5.2 μ m.
Present invention also offers a kind of method that optical 3-dimensional Classical correlation is carried out using above device, including following step
Suddenly：
Step 1, gets out Reference sample and target object sample as the object sample in processing procedure, and
Reference sample, target object sample are performed Step 2: the processing procedure of step 3, then performs step 4 respectively；
Step 2, places uniform source of light in acquisition of information light path, and the light that uniform source of light is sent, which is radiated at, is placed in sample
Object sample on this rotating device, carries out friction free rotation and translation, through object using sample rotating device to sample
The light of sample is exaggerated by light microscope, to collect the three-dimensional information that transmitted light is included with scattering light；Optical microphotograph
The emergent light of mirror is exported using interface arrangement, reaches diffusing transmission element, obtains the three-dimensional information of sample；Light is via unrestrained saturating
The unrestrained projection light penetrated after element is received by microlens array, and unit is carried out using the first ccd sensor after imaged lens focus
Array image gathers, and will gather information record into computer；
Step 3, the laser beam that laser is sent are divided into two-beam line through spectroscope, and the reflected beams on spectroscope are through
It is used as after three mirror reflections and refers to light beam, the projecting beam on spectroscope changes through the first reflective mirror, the second mirror reflection
Behind direction, expanded via upside-down mounting telescopic system and collimation processing, the homogenizer after processing project space light modulation
On device；
Step 4, the cell array with Reference sample three-dimensional information that computer gathers the first ccd sensor
Image is input in spatial light modulator, and spatial light modulator and photorefractive crystal are respectively positioned at preceding Jiao of the first fourier lense
On face and back focal plane, Fourier transformation is carried out through the first fourier lense, is joined on the back focal plane of the first fourier lense
The frequency spectrum of subject image is examined, and is interfered with reference beam in photorefractive crystal, so as to write body in photorefractive crystal
Hologram is matched filtering correlator；
Step 5, the cell array with target object sample three-dimensional information that computer gathers the first ccd sensor
Image is input in spatial light modulator, makes reading of the transmitted light of carrying target object three-dimensional information as matched filtering correlator
Light extraction, carries out inverse Fourier transform by the second fourier lense, reference substance is obtained on the back focal plane of the second fourier lense
The correlation output of body sample and target object sample, is recorded and is input in computer by the second ccd sensor and analyzed, real
Now to the identification of target object.
Embodiment：
The related embodiment of the optical 3-dimensional relevant identification devices based on integrated imaging system is given below.
In the present embodiment, object sample is translucent mould sample, and thickness is about 50 μm, will using sample rotating device
Object sample is fixed on the objective table of optical fiber mirror, and sample rotating device utilizes step motor control, -35 ° of angular range
~35 °, rotate 0.01 ° of resolution ratio；Translating stroke ± 35mm, 1 μm of translation resolution.The lighting source of light microscope is uniform
Light source, 60 times of object lens enlargement ratio, numerical aperture 0.70；10 times of eyepiece enlargement ratio, small items sample is through light microscope
After amplification, the transmitted light comprising three-dimensional information and scattering light are exported using interface arrangement.
Adjustable focus lens focus 15mm, diffusing transmission screen are placed in before adjustable zoom lens in light diffusing transmission element, thickness 2mm,
Granularity is 400, focal length of lens 100mm.The light diffusing transmission element is to the three-dimensional letter of extraction carrying sample from microscope emergent light
The scattering light of breath is simultaneously projected on microlens array.
After microlens array is placed in light diffusing transmission element, to receive diffuse transmission light, size is 60mm × 50mm, by focal length
Formed for the GRIN Lens of 1mm, lenticule size and interval are 1mm.
First ccd sensor is EMCCD, to receive the scattering light via microlens array, pixel under low light condition
Number 512 × 512,16 μm of 16 μ m of pixel dimension.
Laser is 532nm in Classical correlation system, 100mW solid state lasers, and emergent light is adjusted to 18mW through attenuator,
It is divided through spectroscope, object light road, reference path light intensity are respectively 10.8mW, 7.2mW, and it is anti-that reflective mirror 11,12,17 plates high-reflecting film
Penetrate rate 99.8%, the focal length of first Fourier's transmissive mirror and second Fourier's transmissive mirror is 80mm.Photorefractive crystal is 10mm
× 10mm × 10mm, mixes iron 0.03wt%, mixes the LiNbO of zinc 0.6wt%3:Fe crystal.
Be about 50 μm of mould samples as with reference to small items sample using thickness, using light microscope, light diffusing transmission element,
Microlens array, imaging len, the first ccd sensor, the integration imaging three-dimensional microscopic system of computer composition obtain its three-dimensional
Information, inputs to spatial light modulator, and volume hologram is write in photorefractive crystal and makes matched filter.
The another kind of mould sample for being about 50 μm using thickness, Sections of Bone Marrow utilize identical collection as target small items sample
Its three-dimensional information is obtained into imaging three-dimensional microscopic system, is inputted to spatial light modulator, is carried out using optical match filtering method
Classical correlation, for the higher another kind of mould sample of similarity, obtains the cross-correlation output valve more than 0.76；Have for form
The Sections of Bone Marrow sample of larger difference, yields less than 0.48 cross-correlation output valve, so far realizes based on integrated imaging system
Small items three-dimensional Classical correlation.
Test simulation：
Fig. 2, Fig. 3 is obtain the Reference sample of above-described embodiment and target object sample (is treated using apparatus of the present invention
Identify sample) 12 × 12 cell picture arrays, wherein Fig. 2 be certain mould cell picture array image, equal in identification is tested
As with reference to object；Fig. 3 is the cell picture array image of similar mould Different Individual, in high similarity biology microtissue three
Target sample is used as in dimension Classical correlation experiment.
Fig. 4, Fig. 5 are the normalized intensity distribution curve of this three-dimensional identification device output, and wherein Fig. 4 is auto-correlation output
Normalized intensity distribution curve, Fig. 5 are the normalized intensity distribution curve of cross-correlation output.
Claims (7)
1. a kind of optical 3-dimensional relevant identification devices based on integrated imaging system, it is characterised in that obtained including three-dimensional information
Device and Classical correlation system：
Three-dimensional information acquisition device is included on acquisition of information optical path direction spaced sample rotating device (2), light successively
Microscope (3), diffusing transmission element (4), microlens array (5) and imaging len (6) are learned, it is removable on sample rotating device (2)
It is provided with object sample (1) with unloading；Light passes through the object sample (1) on sample rotating device (2) along acquisition of information light path,
Then successively by light microscope (3), diffusing transmission element (4), microlens array (5), imaging len (6) and by first
A computer (8) is passed information to after ccd sensor (7) conversion；
Classical correlation system includes laser (9), and the light that laser (9) is sent is divided into reference path and thing by spectroscope (10)
Light path, is sequentially arranged at intervals with the first reflective mirror (11), the second reflective mirror (12), upside-down mounting telescopic system, sky on object light road
Between optical modulator (15) and the first fourier lense (16)；The 3rd reflective mirror (17) and light are sequentially arranged at intervals with reference path
Late (18), the light in reference path and object light road converge on a photorefractive crystal (19) jointly, and convergence light passes through light
After Photorefractive (19), then expose on the second ccd sensor (21) by the second fourier lense (20), via the 2nd CCD
The computer (8), the spatial light modulator (15) and computer (8) are passed information to after sensor (21) conversion
Connection；
The diffusing transmission element (4) is used for the extraction from light microscope (3) emergent light and carries object sample (1) three-dimensional information
Scattering light and project on microlens array (5)；Diffusing transmission element (4) by along microlens array (5) direction successively
Spaced condenser lens, adjustable zoom lens and unrestrained projection screen composition, wherein the focal length of adjustable zoom lens is 12mm~23mm,
The thickness of unrestrained projection screen is 2mm, and granularity is 400~800mm, and the focal length of condenser lens is 100mm；
In the Classical correlation system, the splitting ratio via the separated object light road of spectroscope (10), reference path is 6:4@
532nm, is coated with high-reflecting film on the first reflective mirror (11), the second reflective mirror (12) and the 3rd reflective mirror (17), high-reflecting film it is anti-
Penetrate rate and be more than 99.8%@532nm；First fourier lense (16), the focal length selection range of second Fourier's transmissive mirror
For 80mm~100mm；
Second ccd sensor (21) is using the high resolution CCD camera for carrying zoom lens, its zoom lens focal length model
Enclose and be more than 1024 × 1024 for 12~36mm, pixel number, pixel dimension is less than 5.2 μm of 5.2 μ m.
2. the optical 3-dimensional relevant identification devices based on integrated imaging system as claimed in claim 1, it is characterised in that described
Sample rotating device (2) include a fixed station for being used for fixed object sample (1), fixed station can do two dimension in the horizontal plane
It is mobile, to realize object sample (1) friction free rotation and translation；The rotation angle range of the fixed station is -180 °
~180 °, rotation resolution ratio is 0.01 °, and translating stroke is ± 100mm, 1 μm of translation resolution.
3. the optical 3-dimensional relevant identification devices based on integrated imaging system as claimed in claim 1, it is characterised in that described
The lighting source of light microscope (3) be uniform source of light, its object lens enlargement ratio is 10~60 times, numerical aperture for 0.3~
0.7, eyepiece enlargement ratio is 10 times.
4. the optical 3-dimensional relevant identification devices based on integrated imaging system as claimed in claim 1, it is characterised in that described
The size of microlens array (5) be 60mm × 50mm, be made of the GRIN Lens that multiple focal lengths are 1mm, self-focusing
A diameter of 1mm of lens, the spacing between GRIN Lens is 1mm.
5. the optical 3-dimensional relevant identification devices based on integrated imaging system as claimed in claim 1, it is characterised in that described
The first ccd sensor (7) use EMCCD, the pixel number of EMCCD is more than 512 × 512, and pixel dimension is less than 20 μm of 20 μ m,
Frame speed>33fps.
6. the optical 3-dimensional relevant identification devices based on integrated imaging system as claimed in claim 1, it is characterised in that described
Photorefractive crystal (19) for size be 10mm × 10mm × 10mm, mix iron 0.03wt%, mix the LiNbO of zinc 0.6wt%3:Fe
Crystal.
7. a kind of method that optical 3-dimensional Classical correlation is carried out using any claim described device in such as claim 1 to 6,
It is characterised in that it includes following steps：
Step 1, gets out Reference sample and target object sample as the object sample in processing procedure, and respectively
Reference sample, target object sample are performed Step 2: the processing procedure of step 3, then performs step 4；
Step 2, places uniform source of light in acquisition of information light path, and the light that uniform source of light is sent, which is radiated at, is placed in sample rotation
Object sample on rotary device, carries out friction free rotation and translation, through object sample using sample rotating device to sample
Light be exaggerated by light microscope, to collect transmitted light and scatter the three-dimensional information that is included of light；Light microscope
Emergent light is exported using interface arrangement, reaches diffusing transmission element, obtains the three-dimensional information of sample；Light is via diffusing transmission member
Unrestrained projection light after part is received by microlens array, and cell array is carried out using the first ccd sensor after imaged lens focus
Image Acquisition, and information record will be gathered into computer；
Step 3, the laser beam that laser is sent are divided into two-beam line through spectroscope, and the reflected beams on spectroscope are anti-through the 3rd
It is used as after light microscopic reflection and refers to light beam, the projecting beam on spectroscope changes direction through the first reflective mirror, the second mirror reflection
Afterwards, expanded via upside-down mounting telescopic system and collimation processing, the homogenizer after processing are projected in spatial light modulator；
Step 4, the cell array image with Reference sample three-dimensional information that computer gathers the first ccd sensor
It is input in spatial light modulator, Fourier transformation is carried out through the first fourier lense, in the back focal plane of the first fourier lense
On obtain the frequency spectrum of Reference image, and interfered with reference beam in photorefractive crystal, so that in photorefractive crystal
Interior write-in volume hologram is as matched filtering correlator；
Step 5, the cell array image with target object sample three-dimensional information that computer gathers the first ccd sensor
It is input in spatial light modulator, makes reading of the transmitted light of carrying target object three-dimensional information as matched filtering correlator
Light, carries out inverse Fourier transform by the second fourier lense, Reference is obtained on the back focal plane of the second fourier lense
The correlation output of sample and target object sample, is recorded and is input in computer by the second ccd sensor and analyzed, and is realized
Identification to target object.
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| CN107543506A (en) * | 2016-06-29 | 2018-01-05 | 魏霖静 | Biological microtissue 3 D information obtaining method based on integration imaging |
| CN107091825A (en) * | 2017-03-31 | 2017-08-25 | 清华大学 | Fluorescent sample chromatography micro imaging method based on microlens array |
| CN107967703B (en) * | 2018-01-05 | 2019-06-21 | 电子科技大学 | A kind of self-focusing of the optical scanner holography based on connected domain and method for reconstructing |
| CN109059787B (en) * | 2018-10-09 | 2019-12-24 | 北京航空航天大学 | Glass plate thickness distribution measuring method and system based on transverse shearing interference |
| EP3942345A1 (en) * | 2019-03-22 | 2022-01-26 | Università degli Studi di Bari "Aldo Moro" | Process and apparatus for the capture of plenoptic images between arbitrary planes |
| CN110824684B (en) * | 2019-10-28 | 2020-10-30 | 华中科技大学 | High-speed three-dimensional multi-modal imaging system and method |
| CN112711030A (en) * | 2020-12-21 | 2021-04-27 | 武汉光目科技有限公司 | Microscope area array sweep frequency measuring device and method |
| CN112947023B (en) * | 2021-02-03 | 2022-06-21 | 昆明理工大学 | Optical scanning holographic three-dimensional object real-time identification system and method |
| CN113325683B (en) * | 2021-05-11 | 2023-01-06 | 昆明理工大学 | Off-axis scanning holographic three-dimensional target real-time joint transformation recognition system and method |
| CN113485082B (en) * | 2021-08-09 | 2022-05-31 | 锋睿领创(珠海)科技有限公司 | Three-dimensional detection system, method, controller and medium based on volume holography |
| CN116755234A (en) * | 2023-08-17 | 2023-09-15 | 浙江荷湖科技有限公司 | Linear light scanning confocal microscopic imaging system and linear light scanning confocal microscopic imaging method |
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