CN105352947A - Urine amino acid metabolite detection reagent preparation method - Google Patents
Urine amino acid metabolite detection reagent preparation method Download PDFInfo
- Publication number
- CN105352947A CN105352947A CN201510552782.4A CN201510552782A CN105352947A CN 105352947 A CN105352947 A CN 105352947A CN 201510552782 A CN201510552782 A CN 201510552782A CN 105352947 A CN105352947 A CN 105352947A
- Authority
- CN
- China
- Prior art keywords
- solution
- amino acid
- preparation
- reagent
- acid metabolite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The present invention relates to the field of detection reagents, and particularly relates to a urine amino acid metabolite detection reagent preparation method comprising the following steps: preparing 0.2-1g / ml nitrous acid copper solution as a solution A; preparing 0.1 to 1 g / ml copper nitrate solution as a solution B; preparing 0.15-1g / ml mercury sulfate solution as a solution C; preparing0.15-1g / ml aluminum sulfate solution as a solution D; mixing the solution A, the solution B, the solution C and the solution D in mass ratio of 1: (0.5-1) :( 0.5-1): 1, adding an ammonium persulfate and sodium bisulfite mixture, and stirring evenly to prepare into a light green urine amino acid metabolite detection reagent. The solution A, the solution B, the solution C and the solution D are mixed in the best mass ratio and the best concentration, and added with the ammonium persulfate and sodium bisulfite mixture for preparing the detection reagent, sensitivity is high, false positive rate and false negative rate are low, the operation is simple, and cost is low.
Description
Technical field
The present invention relates to and detect reagent field, be specifically related to the preparation method that a kind of urine amino acid metabolite detects reagent.
Background technology
Malignant tumour is worldwide disease, is also one of killer that the mankind are the most fiendish, and the number that cancer is died from the whole world is every year about 8,000,000.For many years, domestic and international many experts and scholars have carried out many-sided research and inquirement to the early detection of malignant tumour and diagnosis, Diagnostic Value of Several Serum Tumor Markers sequential use in achieving certain effect to the clinical diagnosis of cancer patient, but they there is complex operation, cost drops into high, accuracy and is subject to the shortcomings such as various factors.And these methods all have wound property mostly, and this extensive examination for disease is unfavorable.Therefore, a kind of human health screening that more can adapt to large-scale crowd fast without wound tumor screening method is set up.Along with the rise of metabolism group, turn to from searching single creature mark and found a specific group mark thing group, thus started the new way of diagnosing tumor technique study.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, provide a kind of highly sensitive, simple to operate, accuracy is high, lower-cost urine amino acid metabolite detects the preparation method of reagent.
The present invention is by the following technical solutions: a kind of urine amino acid metabolite detects the preparation method of reagent, it is characterized in that, comprises the steps:
(1) the cupric nitrite solution of 0.2-1g/ml is prepared, as solution A;
(2) copper nitrate solution of 0.1-1g/ml is prepared, as B solution;
(3) the mercuric sulfate solution of 0.15-1g/ml is prepared, as C solution;
(4) aluminum sulfate solution of 0.15-1g/ml is prepared, as solution D;
(5) by solution A, B solution, C solution, solution D with mass ratio be 1:(0.5-1): (0.5-1): after 1 mixing, add the potpourri of ammonium persulfate and sodium bisulfite, stir and make absinthe-green urine amino acid metabolite and detect reagent.
Aforesaid urine amino acid metabolite detects the preparation method of reagent, and the temperature of the mixed liquor of solution A, B solution, C solution, solution D and solution A, B solution, C solution and solution D is between 15-20 degree Celsius.
Aforesaid urine amino acid metabolite detects the preparation method of reagent, and the preparation process of described solution A is as follows: be dissolved in by 20--25g copper in the nitrous acid solution of the 0.5-1M of 250ml, after copper dissolves completely, make solution A.
Aforesaid urine amino acid metabolite detects the preparation method of reagent, and the preparation process of described B solution is as follows: be dissolved in by 10--25g copper in the salpeter solution of the 2-3M of 250ml, after copper dissolves completely, make B solution.
Aforesaid urine amino acid metabolite detects the preparation method of reagent, and the preparation process of described C solution is as follows: be dissolved in by 15--25g mercury in the sulfuric acid solution of the 2-3M of 250ml, after mercury dissolves completely, make C solution.
Aforesaid urine amino acid metabolite detects the preparation method of reagent, and the preparation process of described solution D is as follows: be dissolved in by 15--25g aluminium in the sulfuric acid solution of the 2-3M of 250ml, after aluminium dissolves completely, make solution D.
Aforesaid urine amino acid metabolite detects the preparation method of reagent, and in step (5), solution A, B solution, C solution, solution D are that 1:1:1:1 mixes with mass ratio.
Aforesaid urine amino acid metabolite detects the preparation method of reagent, and it is 1-2% that the potpourri of ammonium persulfate and sodium bisulfite accounts for the mass percent that urine amino acid metabolite detects reagent; The mass ratio of ammonium persulfate and sodium bisulfite is 1:1.
The present invention adopts solution A, B solution, C solution, solution D with optimum quality ratio and optium concentration mixing and adds that the potpourri of ammonium persulfate and sodium bisulfite is to make detection reagent, highly sensitive, false positive rate and false negative rate low, and cost simple to operate is low; The nitrous acid concentration adopted is lower and preparation temperature is also lower, to make nitrite more stable, and then improves accuracy in detection and sensitivity.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described in detail.
Preparation embodiment 1
(1) cupric nitrite solution is prepared, as solution A; 20g copper is dissolved in the nitrous acid solution of the 1M of 250ml, after copper dissolves completely, makes solution A;
(2) copper nitrate solution is prepared, as B solution; 20g copper is dissolved in the salpeter solution of the 2M of 250ml, after copper dissolves completely, makes B solution;
(3) mercuric sulfate solution is prepared, as C solution; 20g mercury is dissolved in the sulfuric acid solution of the 2M of 250ml, after mercury dissolves completely, makes C solution;
(4) aluminum sulfate solution is prepared, as solution D; 20g aluminium is dissolved in the sulfuric acid solution of the 2M of 250ml, after aluminium dissolves completely, makes solution D.
(5) be after 1:1:1:1 mixing with mass ratio by solution A, B solution, C solution, solution D, add the potpourri of ammonium persulfate and sodium bisulfite, stir and make absinthe-green urine amino acid metabolite and detect reagent.It is 1% that the potpourri of ammonium persulfate and sodium bisulfite accounts for the mass percent that urine amino acid metabolite detects reagent; The mass ratio of ammonium persulfate and sodium bisulfite is 1:1.The temperature of the mixed liquor of solution A, B solution, C solution, solution D and solution A, B solution, C solution and solution D is between 15-20 degree Celsius.
Preparation embodiment 2
(1) cupric nitrite solution is prepared, as solution A; 20g copper is dissolved in the nitrous acid solution of the 1M of 250ml, after copper dissolves completely, makes solution A;
(2) copper nitrate solution is prepared, as B solution; 15g copper is dissolved in the salpeter solution of the 2M of 250ml, after copper dissolves completely, makes B solution;
(3) mercuric sulfate solution is prepared, as C solution; 15g mercury is dissolved in the sulfuric acid solution of the 2M of 250ml, after mercury dissolves completely, makes C solution;
(4) aluminum sulfate solution is prepared, as solution D; 15g aluminium is dissolved in the sulfuric acid solution of the 2M of 250ml, after aluminium dissolves completely, makes solution D.
(5) be after 1:0.5:0.5:1 mixing with mass ratio by solution A, B solution, C solution, solution D, add the potpourri of ammonium persulfate and sodium bisulfite, stir and make absinthe-green urine amino acid metabolite and detect reagent.It is 2% that the potpourri of ammonium persulfate and sodium bisulfite accounts for the mass percent that urine amino acid metabolite detects reagent; The mass ratio of ammonium persulfate and sodium bisulfite is 1:1.The temperature of the mixed liquor of solution A, B solution, C solution, solution D and solution A, B solution, C solution and solution D is between 15-20 degree Celsius.
Preparation embodiment 3
(1) cupric nitrite solution is prepared, as solution A; 20g copper is dissolved in the nitrous acid solution of the 1M of 250ml, after copper dissolves completely, makes solution A;
(2) copper nitrate solution is prepared, as B solution; 25g copper is dissolved in the salpeter solution of the 2M of 250ml, after copper dissolves completely, makes B solution;
(3) mercuric sulfate solution is prepared, as C solution; 25g mercury is dissolved in the sulfuric acid solution of the 2M of 250ml, after mercury dissolves completely, makes C solution;
(4) aluminum sulfate solution is prepared, as solution D; 25g aluminium is dissolved in the sulfuric acid solution of the 2M of 250ml, after aluminium dissolves completely, makes solution D.
(5) be after 1:0.5:0.5:1 mixing with mass ratio by solution A, B solution, C solution, solution D, add the potpourri of ammonium persulfate and sodium bisulfite, stir and make absinthe-green urine amino acid metabolite and detect reagent.It is 1.5% that the potpourri of ammonium persulfate and sodium bisulfite accounts for the mass percent that urine amino acid metabolite detects reagent; The mass ratio of ammonium persulfate and sodium bisulfite is 1:1.The temperature of the mixed liquor of solution A, B solution, C solution, solution D and solution A, B solution, C solution and solution D is between 15-20 degree Celsius.
Comparative example 1
(1) mercuric sulfate solution is prepared, as solution A; 15g mercury is dissolved in the sulfuric acid solution of the 2M of 250ml, after mercury dissolves completely, makes solution A;
(2) nickel nitrate solution is prepared, as B solution; 25g copper is dissolved in the salpeter solution of the 2M of 250ml, after copper dissolves completely, makes B solution.
(3) mercuric nitrate solution is prepared, as C solution; 25g iron is dissolved in the salpeter solution of the 2M of 250ml, after iron dissolves completely, makes C solution.
(4) be after 1:1:1:1 mixing with mass ratio by solution A, B solution, C solution, add antiseptic, stir and make absinthe-green urine amino acid metabolite and detect reagent;
The temperature of the mixed liquor of solution A, B solution, C solution and solution A, B solution, C solution be 15 ?between 20 degrees Celsius.
Comparative example 2
(1) cupric nitrite solution is prepared, as solution A; 20g copper is dissolved in the nitrous acid solution of the 1M of 250ml, after copper dissolves completely, makes solution A;
(2) copper nitrate solution is prepared, as B solution; 25g copper is dissolved in the salpeter solution of the 2M of 250ml, after copper dissolves completely, makes B solution;
(3) mercuric sulfate solution is prepared, as C solution; 25g mercury is dissolved in the sulfuric acid solution of the 2M of 250ml, after mercury dissolves completely, makes C solution;
(4) aluminum sulfate solution is prepared, as solution D; 25g aluminium is dissolved in the sulfuric acid solution of the 2M of 250ml, after aluminium dissolves completely, makes solution D.
(5) be after 1:0.5:0.5:1 mixing with mass ratio by solution A, B solution, C solution, solution D, add the potpourri of ammonium persulfate and sodium bisulfite, stir and make absinthe-green urine amino acid metabolite and detect reagent.It is 1.5% that the potpourri of ammonium persulfate and sodium bisulfite accounts for the mass percent that urine amino acid metabolite detects reagent; The mass ratio of ammonium persulfate and sodium bisulfite is 1:1.The temperature of the mixed liquor of solution A, B solution, C solution, solution D and solution A, B solution, C solution and solution D be 30 ?35 degrees Celsius.
Comparative example 3
(1) cupric nitrite solution is prepared, as solution A; 20g copper is dissolved in the nitrous acid solution of the 1M of 250ml, after copper dissolves completely, makes solution A;
(2) copper nitrate solution is prepared, as B solution; 25g copper is dissolved in the salpeter solution of the 2M of 250ml, after copper dissolves completely, makes B solution;
(3) mercuric sulfate solution is prepared, as C solution; 25g mercury is dissolved in the sulfuric acid solution of the 2M of 250ml, after mercury dissolves completely, makes C solution;
(4) aluminum sulfate solution is prepared, as solution D; 25g aluminium is dissolved in the sulfuric acid solution of the 2M of 250ml, after aluminium dissolves completely, makes solution D.
(5) be after 1:0.5:0.5:1 mixing with mass ratio by solution A, B solution, C solution, solution D, stir and make absinthe-green urine amino acid metabolite and detect reagent.The temperature of the mixed liquor of solution A, B solution, C solution, solution D and solution A, B solution, C solution and solution D be 15 ?20 degrees Celsius.
Get 100 hepatocarcinoma patient urine specimens made a definite diagnosis and 10 without cancer sample (i.e. negative sample), carry out the detection of urine amino acid metabolite with preparation embodiment 1 ?3 and comparative example 1 ?3 respectively, testing result is as following table 1 and table 2.
Detection method is: get examinee urine 3ml, in the detection reagent of instillation 0.5ml, shake up, leave standstill 3min, examination criteria is: occurring white/yellow precipitation or do not occur precipitation person, is feminine gender, occur that grey/black precipitate person is positive, can arrange color board to by grey to the different colours of black, color illustrates that amino acid metabolite content is more more deeply, and tumour may be more serious.Wherein color grade 0 is negative findings, and all the other color grades are grey or black.
The testing result of table 1 pair hepatocarcinoma patient
The testing result of table 2 pair negative sample
As shown in Table 1, detection reagent of the present invention is about 95% to the recall rate of hepatocarcinoma patient, higher than existing detection reagent.As shown in Table 2, detection reagent of the present invention can not cause false positive, is also better than existing detection reagent.
It should be noted that; the foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other correlative technology fields, be all in like manner included in scope of patent protection of the present invention.
Claims (8)
1. urine amino acid metabolite detects a preparation method for reagent, it is characterized in that, comprises the steps:
The cupric nitrite solution of preparation 0.2-1g/ml, as solution A;
The copper nitrate solution of preparation 0.1-1g/ml, as B solution;
The mercuric sulfate solution of preparation 0.15-1g/ml, as C solution;
The aluminum sulfate solution of preparation 0.15-1g/ml, as solution D;
Be 1:(0.5-1 with mass ratio by solution A, B solution, C solution, solution D): (0.5-1): after 1 mixing, add the potpourri of ammonium persulfate and sodium bisulfite, stirs and makes absinthe-green urine amino acid metabolite and detect reagent.
2. urine amino acid metabolite according to claim 1 detects the preparation method of reagent, it is characterized in that,
The temperature of the mixed liquor of solution A, B solution, C solution, solution D and solution A, B solution, C solution and solution D is between 15-20 degree Celsius.
3. urine amino acid metabolite according to claim 1 detects the preparation method of reagent, it is characterized in that, the preparation process of described solution A is as follows: be dissolved in by 20--25g copper in the nitrous acid solution of the 0.5-1M of 250ml, after copper dissolves completely, make solution A.
4. urine amino acid metabolite according to claim 1 detects the preparation method of reagent, and it is characterized in that, the preparation process of described B solution is as follows: be dissolved in by 10--25g copper in the salpeter solution of the 2-3M of 250ml, after copper dissolves completely, make B solution.
5. urine amino acid metabolite according to claim 1 detects the preparation method of reagent, and it is characterized in that, the preparation process of described C solution is as follows: be dissolved in by 15--25g mercury in the sulfuric acid solution of the 2-3M of 250ml, after mercury dissolves completely, make C solution.
6. urine amino acid metabolite according to claim 1 detects the preparation method of reagent, and it is characterized in that, the preparation process of described solution D is as follows: be dissolved in by 15--25g aluminium in the sulfuric acid solution of the 2-3M of 250ml, after aluminium dissolves completely, make solution D.
7. urine amino acid metabolite according to claim 1 detects the preparation method of reagent, and it is characterized in that, in step (5), solution A, B solution, C solution, solution D are that 1:1:1:1 mixes with mass ratio.
8. urine amino acid metabolite according to claim 1 detects the preparation method of reagent, it is characterized in that, it is 1-2% that the potpourri of ammonium persulfate and sodium bisulfite accounts for the mass percent that urine amino acid metabolite detects reagent; The mass ratio of ammonium persulfate and sodium bisulfite is 1:1.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510552782.4A CN105352947A (en) | 2015-09-02 | 2015-09-02 | Urine amino acid metabolite detection reagent preparation method |
| PCT/CN2015/090538 WO2017035882A1 (en) | 2015-09-02 | 2015-09-24 | Method for preparing urine amino acid metabolite detection reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510552782.4A CN105352947A (en) | 2015-09-02 | 2015-09-02 | Urine amino acid metabolite detection reagent preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105352947A true CN105352947A (en) | 2016-02-24 |
Family
ID=55328953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510552782.4A Pending CN105352947A (en) | 2015-09-02 | 2015-09-02 | Urine amino acid metabolite detection reagent preparation method |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN105352947A (en) |
| WO (1) | WO2017035882A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU231258B1 (en) * | 2019-01-28 | 2022-07-28 | Ioi Auranae Kft. | Device and method for automatic determination of anion and cation level in urine |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1068946C (en) * | 1997-01-30 | 2001-07-25 | 傅敏恭 | Cancer uroscopy colorimetric method and device |
| CN102368069A (en) * | 2011-03-25 | 2012-03-07 | 吉林生物研究院有限公司 | Hydroxyphenylalanine urine detection reagent and preparation method thereof |
| CN104390975B (en) * | 2014-10-31 | 2016-03-02 | 江苏东博生物医药有限公司 | In urine, tumor markers 5-hydroxyindoleacetic acid detects reagent and preparation method |
| CN104535565B (en) * | 2014-12-25 | 2016-03-02 | 江苏东博生物医药有限公司 | Single hydroxyl phenolic metabolism thing urine detection reagent and preparation method thereof |
| CN104597046A (en) * | 2015-01-21 | 2015-05-06 | 重庆都好生物科技有限公司 | Cancer urine detection reagent |
-
2015
- 2015-09-02 CN CN201510552782.4A patent/CN105352947A/en active Pending
- 2015-09-24 WO PCT/CN2015/090538 patent/WO2017035882A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017035882A1 (en) | 2017-03-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102004103B (en) | Kit for detecting tyrosine phenol metabolites in human urine | |
| CN104390974B (en) | In a kind of urine, tumor markers tryptophane detects reagent and preparation method | |
| CN104390975B (en) | In urine, tumor markers 5-hydroxyindoleacetic acid detects reagent and preparation method | |
| CN102565055B (en) | Preparation method for urine monohydroxyphenyl metabolites detection reagent | |
| CN103483872B (en) | For the coloring composition and preparation method thereof of tumor tissue cell | |
| CN103323452A (en) | P-hydroxy phenylalanine urine detection reagent and preparation method thereof | |
| CN105158248A (en) | Preparation method of single hydroxyl phenolic urine metabolite detection reagent | |
| CN102368069A (en) | Hydroxyphenylalanine urine detection reagent and preparation method thereof | |
| CN106370649B (en) | The whether exceeded rapid detection method of content of nitrite in a kind of processing meat product | |
| CN104698164B (en) | A kind of Electrogenerated chemiluminescent immunoassay instrument buffer and preparation method thereof | |
| CN110108701A (en) | A kind of lead ion rapid detection method based on fluorescence colorimetric and microflow control technique | |
| CN101934380B (en) | Preparation method of immune colloidal gold particles capable of being used for rapid diagnosis | |
| CN103760121A (en) | Method for detecting nitrite in blood | |
| CN111024665A (en) | Method for measuring content of organic selenium in selenium-enriched rice or product prepared from selenium-enriched rice | |
| CN105136793A (en) | Preparation method of reagent for detecting hydroxyl phenylalanine metabolite from urine | |
| CN105352947A (en) | Urine amino acid metabolite detection reagent preparation method | |
| CN104237188A (en) | Fluorescent probe for simply and quickly detecting zinc ions | |
| CN108008060A (en) | The assay method and reagent of hydroxyproline in a kind of feed | |
| CN105527284A (en) | Reagent for detecting tyrosine tumor markers in human urine | |
| CN106405067B (en) | A kind of detection method of the drug target residence time based on full cell | |
| CN104406975A (en) | Method for preparing detection reagent of para hydroxyl phenylalanine in urea | |
| CN106916174A (en) | A kind of chiral amino acid derivative complex, preparation method and applications | |
| CN104914096A (en) | Clenbuterol detection operating fluid and detection method thereof | |
| CN110849869A (en) | Color development method and kit for detecting copper ion concentration limit in radioactive injection | |
| CN105779559A (en) | Liquid-based cell treatment solution |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160224 |