CN102004103B - Kit for detecting tyrosine phenol metabolites in human urine - Google Patents
Kit for detecting tyrosine phenol metabolites in human urine Download PDFInfo
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- CN102004103B CN102004103B CN2010105057934A CN201010505793A CN102004103B CN 102004103 B CN102004103 B CN 102004103B CN 2010105057934 A CN2010105057934 A CN 2010105057934A CN 201010505793 A CN201010505793 A CN 201010505793A CN 102004103 B CN102004103 B CN 102004103B
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- nitrate solution
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- mercuric
- kit
- solution
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- 210000002700 urine Anatomy 0.000 title claims abstract description 17
- YTBKOFZZSHCXGJ-QRPNPIFTSA-N (2s)-2-amino-3-(4-hydroxyphenyl)propanoic acid;phenol Chemical class OC1=CC=CC=C1.OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 YTBKOFZZSHCXGJ-QRPNPIFTSA-N 0.000 title abstract 2
- ORMNPSYMZOGSSV-UHFFFAOYSA-N dinitrooxymercury Chemical compound [Hg+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ORMNPSYMZOGSSV-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 17
- DOBUSJIVSSJEDA-UHFFFAOYSA-L 1,3-dioxa-2$l^{6}-thia-4-mercuracyclobutane 2,2-dioxide Chemical compound [Hg+2].[O-]S([O-])(=O)=O DOBUSJIVSSJEDA-UHFFFAOYSA-L 0.000 claims abstract description 12
- 229940074994 mercuric sulfate Drugs 0.000 claims abstract description 12
- 229910000372 mercury(II) sulfate Inorganic materials 0.000 claims abstract description 12
- KBJMLQFLOWQJNF-UHFFFAOYSA-N nickel(ii) nitrate Chemical compound [Ni+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O KBJMLQFLOWQJNF-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 12
- 230000004060 metabolic process Effects 0.000 claims description 11
- 239000012153 distilled water Substances 0.000 claims description 6
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 6
- 229910052753 mercury Inorganic materials 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 4
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 3
- 239000007795 chemical reaction product Substances 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 229910017604 nitric acid Inorganic materials 0.000 claims description 3
- 125000001493 tyrosinyl group Chemical class [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 6
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 239000012030 Millon's reagent Substances 0.000 abstract description 2
- 239000003513 alkali Substances 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract 2
- 229910021645 metal ion Inorganic materials 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 description 21
- 201000011510 cancer Diseases 0.000 description 13
- 150000003668 tyrosines Chemical class 0.000 description 10
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 6
- 230000008021 deposition Effects 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000005303 weighing Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 206010027336 Menstruation delayed Diseases 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 230000037354 amino acid metabolism Effects 0.000 description 1
- 239000011449 brick Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 150000002730 mercury Chemical class 0.000 description 1
- 230000037323 metabolic rate Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 150000001455 metallic ions Chemical class 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- SPIFDSWFDKNERT-UHFFFAOYSA-N nickel;hydrate Chemical compound O.[Ni] SPIFDSWFDKNERT-UHFFFAOYSA-N 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a kit for detecting tyrosine phenol metabolites in human urine, comprising a reagent, wherein the reagent is a light green transparent liquid formed by mixing 0.1-0.2 g/ml of mercuric sulfate solution, 1-2 g/ml of mercuric nitrate solution, 0.1-0.2 g/ml of nickel nitrate solution in the volume ratio of 1:0.5-1.5:0.5-2. The kit is innovated on the basis of the principle of a Millon reagent, eliminates the interferences of alkali substances, metal ions and other relevant substances in the urine on reagent detection, and improves the detection sensitivity and accuracy.
Description
Technical field
The present invention relates to a kind of kit that is used for human body urine tyrosine class phenol metabolism thing.
Technical background
Early detection is the key of treatment tumour, and the development of tumour is divided into five stages: optimum, tumour cell starting period, tumour early stage, tumour mid-term, late period.The fearful part of cancer just is preceding three phases, and iconography can't detect at all, even if arrived tumour mid-term, also having only very, the doctor of specialty just can measure with advanced equipment.The research of the World Health Organization (WHO) is thought: present 1/3 cancer can be prevented; 1/3 cancer can prolong patient's life through active treatment; 1/3 cancer can be fully recovered through early detection, early diagnosis, early treatment.The characteristic amino acid metabolism is unusual in the urine of cancer patient, produces a large amount of tyrosine class phenol metabolism things.Detect the content of tyrosine class phenol metabolism thing, can infer whether human body suffers from cancer.
Summary of the invention
The object of the invention just provides the kit that is used for human body urine tyrosine class phenol metabolism thing of a kind of detection sensitivity height, accuracy good.
The kit that is used for human body urine tyrosine class phenol metabolism thing of the present invention; Comprise reagent; Reagent by the nickel nitrate solution of the mercuric nitrate solution of the mercuric sulfate solution of 0.1-0.2g/ml, 1-2g/ml, 0.1-0.2g/ml by volume; Be mercuric nitrate solution: mercuric sulfate solution: nickel nitrate solution=1: 0.5～1.5: 0.5～2, mix, be the light green transparency liquid.
The mercuric sulfate solution of the compound method of each component: 0.1-0.2g/ml calculates by concentration in the reagent, accurately takes by weighing the amount of mercuric sulfate (analyzing pure) reagent, is dissolved in the sulfuric acid solution of 3mol/L, is mixed with the mercuric sulfate solution of this concentration; It is that the ratio of 1.4 nitric acid 30ml is reacted that the mercuric nitrate solution of 1-2g/ml uses 20g mercury and proportion, treat that mercury dissolves fully after, add 1-2 times to the distilled water of this reaction product volume, be mixed with the mercuric nitrate solution of this concentration; 0.1-0.2g/ml nickel nitrate solution calculate by concentration, accurately take by weighing the amount of nickel nitrate (analyzing pure) reagent, be dissolved in the distilled water, be mixed with the nickel nitrate solution of this concentration.
The kit that is used for human body urine tyrosine class phenol metabolism thing of the present invention; Detection method: get examinee's urine 3ml, be added drop-wise in the test tube that this reagent 0.5ml is housed, left standstill 2-5 minute; Deposition and color reaction take place, according to the color judgment that has or not and precipitate of deposition.
Criterion: 1, if reaction does not have deposition, or deposition is arranged, color is a white or yellow, is judged as feminine gender, does not have cancer in the prompting body; 2, if reaction has deposition, color is rose-red or brick red, is judged as the positive, in the prompting body cancer is arranged.
Medical science and clinical research show; The high reproductive rate of cancer cell and high metabolic rate make cancer patient's urine a large amount of amino acid (tyrosine) metabolins that contains phenol nuclear occur; And this type of amino acid metabolite of extremely low concentration does not appear in normal person's urine or only contains; Therefore, tyrosine class phenol metabolism thing becomes one of tumor markers.Mercury salt in this reagent is the tumor markers specific reaction therewith; Reaction product generates red precipitate through molecule isomery and oxidation; And do not have deposition with normal person's urine reaction or sediment be a yellow; Whether whether the existence of judging this type of tumor markers through sediment and color thereof and then infers whether carry cancer cell, be the cancer patient promptly.
The kit that is used for human body urine tyrosine class phenol metabolism thing of the present invention; Be on Millon reagent principle basis, to innovate; It has got rid of urine neutral and alkali material, metallic ion and other related substanceses to the interference that reagent detects, and has improved the sensitivity and the accuracy that detect, the clinical verification through tumour hospital; 300 examples are and in the cancer patient that histology is made a definite diagnosis, detect 278 routine positive patients, and accuracy rate (coincidence rate) reaches 92.6%.
Embodiment
A kind of kit that is used for human body urine tyrosine class phenol metabolism thing, prepare the reagent method of this kit 1000ml:
Reagent is by three kinds of solution compositions, by volume ratio, i.e. mercuric nitrate solution: mercuric sulfate solution: nickel nitrate solution=1: 1: 2, be formulated as 250ml respectively, and 250ml and 500ml mix three kinds of solution and promptly are mixed with this reagent of 1000ml.
The compound method of each component is following:
1, the mercuric nitrate solution concentration is 1g/ml, takes by weighing 64g mercury and is dissolved among the nitric acid 96ml of proportion 1.4, treats that mercury dissolves fully, adds the distilled water of 166.7ml, promptly is mixed with 250ml, and concentration is the mercuric nitrate solution of 1g/ml;
2, the mercuric sulfate solution concentration is 0.1g/ml, takes by weighing 25g mercuric sulfate reagent, is dissolved in the sulfuric acid solution of a spot of 3mol/L, and the sulfuric acid solution that adds 3mol/L again is to 250ml, and promptly being mixed with concentration is the mercuric sulfate solution of 0.1g/ml;
3, nickel nitrate solution concentration is 0.1g/ml, takes by weighing 6 water nickel nitrate 80g, is dissolved in a small amount of distilled water, adds distilled water again to 500ml, promptly is mixed with the nickel nitrate solution that concentration is 0.1g/ml.
Claims (1)
1. kit that is used for human body urine tyrosine class phenol metabolism thing; Comprise reagent; It is characterized in that: reagent by the nickel nitrate solution of the mercuric nitrate solution of the mercuric sulfate solution of 0.1-0.2g/ml, 1-2g/ml, 0.1-0.2g/ml by volume; Be mercuric nitrate solution: mercuric sulfate solution: nickel nitrate solution=1: 0.5～1.5: 0.5～2, mix, be the light green transparency liquid; Wherein the compound method of the mercuric nitrate solution of 1-2g/ml is: using 20g mercury and proportion is that the ratio of 1.4 nitric acid 30ml is reacted, treat that mercury dissolves fully after, add 1-2 times to the distilled water of this reaction product volume, be mixed with the mercuric nitrate solution of this concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105057934A CN102004103B (en) | 2010-10-13 | 2010-10-13 | Kit for detecting tyrosine phenol metabolites in human urine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105057934A CN102004103B (en) | 2010-10-13 | 2010-10-13 | Kit for detecting tyrosine phenol metabolites in human urine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102004103A CN102004103A (en) | 2011-04-06 |
| CN102004103B true CN102004103B (en) | 2012-02-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105057934A Active CN102004103B (en) | 2010-10-13 | 2010-10-13 | Kit for detecting tyrosine phenol metabolites in human urine |
Country Status (1)
| Country | Link |
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| CN (1) | CN102004103B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10234397B2 (en) * | 2014-12-25 | 2019-03-19 | Jiangsu Dongbo Bio-Pharmaceutical Co. Ltd. | Monohydroxyphenyl metabolite urine detection reagent and preparation method thereof |
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| CN102565055B (en) * | 2012-01-16 | 2013-10-30 | 李德海 | Preparation method for urine monohydroxyphenyl metabolites detection reagent |
| CN103323452B (en) * | 2013-06-21 | 2014-07-09 | 吉林生物研究院有限公司 | P-hydroxy phenylalanine urine detection reagent and preparation method thereof |
| CN104390975B (en) * | 2014-10-31 | 2016-03-02 | 江苏东博生物医药有限公司 | In urine, tumor markers 5-hydroxyindoleacetic acid detects reagent and preparation method |
| CN104390974B (en) * | 2014-10-31 | 2016-03-02 | 江苏东博生物医药有限公司 | In a kind of urine, tumor markers tryptophane detects reagent and preparation method |
| US10845361B2 (en) | 2015-02-05 | 2020-11-24 | Ath (Australia) Pty Ltd | Formulations comprising mercury-containing reagents for detecting disease |
| CN104949967B (en) * | 2015-06-09 | 2016-08-24 | 杭州欣叶生物科技有限公司 | The kit of tyrosine phenol metabolism thing in detection human urine |
| CN105044100A (en) * | 2015-07-28 | 2015-11-11 | 杭州欣叶生物科技有限公司 | Kit for detecting tyrosine phenolic metabolite in human urine |
| CN105181687A (en) * | 2015-08-28 | 2015-12-23 | 朱建华 | Hydroxyl phenylalanine tyrosine urine detecting kit |
| CN105136793A (en) * | 2015-09-21 | 2015-12-09 | 苏州锐霖生物科技有限公司 | Preparation method of reagent for detecting hydroxyl phenylalanine metabolite from urine |
| CN105203537A (en) * | 2015-09-23 | 2015-12-30 | 杭州欣叶生物科技有限公司 | Urine testing reagent for tyrosine phenol metabolin |
| CN105527284A (en) * | 2015-12-21 | 2016-04-27 | 北京北斗星航生物科技有限公司 | Reagent for detecting tyrosine tumor markers in human urine |
| CN105628692A (en) * | 2015-12-22 | 2016-06-01 | 大连一科生物科技有限公司 | Reagent for rapidly detecting small molecular phenol component in urine and preparation technology |
| CN105866113A (en) * | 2016-04-18 | 2016-08-17 | 杨祖平 | Preparation method of urinalysis reagent kit with phenol nucleus amino acid and derivative thereof |
| CN106093033A (en) * | 2016-06-15 | 2016-11-09 | 江西格朗生物技术有限公司 | The preparation method of broad-spectrum tumor urinalysis reagent |
| CN106226299A (en) * | 2016-08-15 | 2016-12-14 | 李永福 | For detecting the test kit of tyrosine phenol metabolism thing |
| CN107064482B (en) * | 2017-04-26 | 2019-03-08 | 上海菲济生物科技有限公司 | A kind of list hydroxyl phenolic metabolism object human urine detection reagent |
| CN107084977B (en) * | 2017-04-26 | 2020-02-18 | 浙江欣叶健康管理有限公司 | Reagent for detecting tyrosine phenol metabolites in urine and product thereof |
| CN109115764B (en) * | 2018-07-30 | 2021-06-15 | 深圳瑞达生物股份有限公司 | Environment-friendly urine hydroxyphenyl derivative detection reagent and preparation method thereof |
| CN109406502B (en) * | 2018-10-22 | 2021-01-26 | 南京邮电大学 | Detection reagent for phenolic amino acid-containing solution |
| CN109470696A (en) * | 2018-12-27 | 2019-03-15 | 冀晓玲 | A kind of detection reagent and preparation method thereof of quick detection urine para hydroxybenzene alanine |
| CN109682807B (en) * | 2019-03-06 | 2021-09-21 | 武汉福朗科技发展有限公司 | Monohydroxyphenol urinalysis kit capable of resisting interference of urine components, and preparation method, operation method and application thereof |
| CN112213304A (en) * | 2020-10-16 | 2021-01-12 | 深圳市宸果材料科技有限公司 | Urine sieve cancer kit based on color comparison |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0341803A1 (en) * | 1988-03-11 | 1989-11-15 | SAM I1 PHARMACEUTICAL MANUFACTURING CO., Ltd | Improved Millon's reagent for cancer screening diagnosis |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1053043C (en) * | 1996-08-16 | 2000-05-31 | 施瑞生物技术(深圳)有限公司 | Fast urine testing reagent for cancer |
-
2010
- 2010-10-13 CN CN2010105057934A patent/CN102004103B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0341803A1 (en) * | 1988-03-11 | 1989-11-15 | SAM I1 PHARMACEUTICAL MANUFACTURING CO., Ltd | Improved Millon's reagent for cancer screening diagnosis |
Non-Patent Citations (1)
| Title |
|---|
| Manki Park，et al..Development of Reagent for Cancer Diagnosis by Urine Color Reaction (D-Comparative analysis of cancer and non-cancer urine by NMR, HPLC and Gift reagent.《Arch. Pharm. Res.》.1988,第11卷(第2期),134-138. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10234397B2 (en) * | 2014-12-25 | 2019-03-19 | Jiangsu Dongbo Bio-Pharmaceutical Co. Ltd. | Monohydroxyphenyl metabolite urine detection reagent and preparation method thereof |
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| Publication number | Publication date |
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| CN102004103A (en) | 2011-04-06 |
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Effective date of registration: 20131120 Address after: 330096, No. four, West 555, hi tech Avenue, Nanchang hi tech Zone, Jiangxi Patentee after: JIANGXI HUAYUKANG BIOTECHNOLOGY CO., LTD. Address before: 330039 Nanchang City, Jiangxi Province, Qingshan District, Jiangsu Province, Huayuan Road HUAYU Jiangxi Biological Technology Co., Ltd. Patentee before: Zhu Jianhua |