CN105349437A - High color value monascorubin strain and production process thereof - Google Patents

Abstract

The invention relates to a high color value monascorubin strain and a production process thereof. The strain is preserved in Typical Culture Preservation Centre in China on Sep. 17, 2014, with a preservation number of CCTCC NO: M2014420. The strain is prepared by a vacuum cryovial, a test tube slant strain, a triangular shake flask strain and a strain tank strain through the steps of strain stirring, piling, spreading, fermentation culturing, drying and the like. Liquid-solid culture fermentation is adopted, the product is high in pigment purity, has better stability to light, heat and oxygen, contains multiple pigment components and functional components, has color developing, anticorrosive and health care functions, is mainly applied to coloring and anticorrosion in machining of various foods, meat products and seasonings, is particularly applied to the meat products, and can replace sudan red and nitrate which can generate carcinogenesis to a human body.

Description

A kind of High color values monascorubin bacterial classification and production technique thereof

Technical field

The present invention relates to technical field of bioengineering, particularly a kind of High color values monascorubin bacterial classification and production technique thereof.

Background technology

Existing food dye is divided into synthetic colour and the large class of natural pigment two, the lovely luster of synthetic colour is stablized, with low cost, easy to use, but because synthetic colour is mostly tar series of products, or have that the material such as phenyl ring or naphthalene nucleus synthesizes by contained in coal tar, its Chemical Composition directly can damage HUMAN HEALTH, or objectionable impurities is produced in human body metabolism, particularly likely cause canceration and cause all illnesss, now restriction uses or prohibits the use gradually, therefore by animal, that extracts in plant and microorganism is nontoxic edible, the edible natural pigment not damaging HUMAN HEALTH is more and more subject to the attention of people or society.

Monascorubin is the secondary metabolite of monascus, is considered to the food dye that security is higher all the time, and test has proved not containing aflatoxin, also without mutagenesis.Along with nineteen ninety-five France scholar P.J.Blanc etc. detects a kind of mycotoxins be harmful to people and animals in Monascus anka Nakazawa et sato tunning--Citrinin (Citrinin), monascorubin receives the concern of people as the safety problem of foodstuff additive.

Existing production monascorubin major part directly inoculates the Simple process method of solid state bacterial, aerated koji making, and it is lower that the monascorubin that this processing method is produced exists painted valency, light or thermostability poor, its look valency is also not too high, produces the shortcomings such as look ability is more weak.

Summary of the invention

In order to overcome the deficiencies in the prior art, the invention provides a kind of High color values monascorubin bacterial classification and production technique thereof, solving the light that exists in prior art or thermostability is poor, look valency is not high and produce the technical problems such as look ability is more weak.

The technical solution adopted for the present invention to solve the technical problems is:

A kind of High color values monascorubin bacterial classification, this bacterial classification is preserved in China typical culture collection center on September 17th, 2014, and deposit number is: CCTCCNO:M2014420.

Gu the production technique that the liquid-fermentation of above-mentioned High color values monascorubin bacterial classification combines, comprise the following steps:

The preservation in 4 DEG C of refrigerators of step [1] vacuum freezing pipe;

Step [2] strain expanded culture:

Test tube slant bacterial classification: adopt improvement czapek's solution, cultivate 6 ~ 8 days → triangle shake-flask seed for 32 DEG C ± 2 DEG C: adopt glucose two substratum, cultivate 48 ~ 72 hours → seeding tank seed for 32 DEG C ± 2 DEG C: adopt ground rice substratum, 32 DEG C ~ 40 DEG C, air flow 7 ~ 14m ³/ h, cultivate 24 ~ 48 hours, obtain secondary seed solution, described seed liquor is made up of the component of following weight ratio: JC-SM12 bacterial classification 0.0005%-0.0015%, ground rice 0.085%-0.090%, yeast extract paste 0.014%-0.024%, water 99.85%-99.95%, step through above-mentioned enlarged culturing step by step obtains production second class inoculum, in culturing process, need sterile sampling to monitor growth situation, determine bacterial classification maturation time, after liquid seeds cultivates maturation, sterile sampling is needed, assessment seed liquor quality condition before entering subsequent processing.

Production second class inoculum specification of quality: seed liquor presents pink to red-purple, comparatively thickness, and mycelium pellet is more, even in paper paddle, free from extraneous odour; Microscopy mycelium is sturdy, even, tabula is clear, and spore (pyriform or spherical) is more, without miscellaneous bacteria; PH value lower than 7, generally 6.0 ~ 6.5.Through streak culture, except the colonies typical of object bacteria, grow without other miscellaneous bacterias.

Immersion, the boiling of step [3] rice and the rice that dries in the air

Rice is placed in puffed wheat device, soaks;

The puffed wheat time: annual May ~ September puffed wheat 8 ~ 10h, October ~ April next year puffed wheat 10 ~ 12h;

Amount of water requires: the water surface did not have material 5 ~ 10 centimetres;

Pour soaked rice into meal braizing machine, charging capacity 300kg/ pot, require that the rice amount dropping into meal braizing machine is no more than meal braizing machine outer; Open steam, after vapour on material, start timing, the 15 minutes vapour time in maintenance.

Ripe rice specification of quality after steaming: well-done without the white heart, soft not rotten not conglomeration, moisture content 32% ~ 35%;

Dry in the air rice: environmentally temperature grasps the temperature of material flexibly, and when envrionment temperature is lower than 20 DEG C, before inoculation, temperature of charge can be controlled between 38 DEG C ~ 40 DEG C; When envrionment temperature is higher than 20 DEG C, before inoculation, temperature of charge can be controlled between 35 DEG C ~ 38 DEG C; The rice dried well is input to inoculation device;

Step [4] is dressed seed:

Inoculate pipeline with steam sterilizing, in secondary seed solution, add food grade lactic acid or food grade Glacial acetic acid that weight percent is 0.1% of rice raw material charging capacity, fully mix, secondary seed solution is introduced inoculation device for subsequent use;

Open inoculation device, regulate seed liquor flow, ensure the homogeneity of inoculum size 6 ~ 8% and the rear material of inoculation, the temperature control of inoculum item is at 32 ~ 35 DEG C;

The sack splendid attire of material Turnover Box or cleaning after inoculation, is transported to Quchi (temperature is higher than 20 DEG C) or high hot house (temperature is lower than 20 DEG C) with transport pushing cart.

Step [5] beats heap:

Pile up in time in Quchi or high hot house by material after inoculation, every pond charging capacity 750 ~ 800kg, the side's of scrabbling heap, piles thick about 40 ~ 50cm, observes at any time and records windrow temperature variations, when beating stacking yard item temperature and reaching 45 DEG C, can enter next process.

Under normal circumstances, windrow temperature rose at 15 ~ 24 hours and requires product temperature (45 DEG C).

Step [6] stand heap is grown flower:

When beating stacking yard item temperature and reaching 45 DEG C, or high hot house can be broken heap material and proceed to fermentation vat and carry out stand heap and grow flower at Quchi, product temperature when maintenance is grown flower is at 35 ~ 42 DEG C, depending on material product temperature rising speed, successively make thinner, until full full pond, stand, behind full full pond, stand, particular case depending on growing flower takes counter-measure (as local growing way does not change, then to want detected temperatures, moisture whether to occur exception, and adjust in time for abnormal in time; As whole pond growing state stops, then should analyze reason immediately; Problem like this, needs the exception that site operation personnel arrives according to the observation, handles it in time) control material product temperature at 35 ~ 38 DEG C, ensure at interval of 4 hours thorough stirrings once.

Step [7] fermentation culture:

One, add water operation and requirement

1, Jia Yishui

Add water the time: generally 24 hours after the heap of stand, grow flower neat.Organoleptic requirements: white hypha is evenly infiltrated in 90% grain of rice surface, and the part grain of rice reddens.Now monascus high-speed rapid growth, thermal value is comparatively large, need repeatedly to add a water on a small quantity, and amount of water is larger.

Water feeding method: while add water, limit stirring, loose depending on bent material, dry wet situation, repeats above-mentioned steps, until material is evenly moisture after adding water.

Control overflow:

1) after adding water 1 hour, on moistening, the Quchi gauze of material and material surface is without free-water.Moisture is (4 ~ October, moisture can remain between 43% ~ 48%, and March November to next year, moisture can remain between 40% ~ 45%) between 40% ~ 48%.

2) control product temperature 33 DEG C ~ 35 DEG C after a water is added.

2, Jia Ershui

Add water the time: the growing state depending on material determines to add the time of two water, after a water 10 hours.

Water feeding method: while add water, limit stirring, loose depending on bent material, dry wet situation, repeats above-mentioned steps, until material is evenly moisture after adding water.

Control overflow: moisture controlled is 43 ~ 50%, and product temperature control at about 33 DEG C, and notices that this stage low temperature must not lower than 30 DEG C, and high temperature must not more than 34 DEG C.

3, culturing process adds water the intermediary and later stages

Intermediary and later stages culturing process water feeding method and requirement are with two water;

4, go out pond to dry and add water the day before yesterday

Go out pond whether to add water and according to water content detection result on the same day, if moisture is more than 43%, then need not will add water the day before yesterday, otherwise suitably moisturizing, after moisturizing, moisture requirement is no more than 48%, and product temperature control is at about 33 DEG C, and low temperature must not lower than 30 DEG C, and high temperature must not more than 34 DEG C.

Two, stirring operation and requirement

Within after adding water about one hour, require again that thoroughly stirring once.Stirring afterwards will depending on the growing state of material, but must ensure to want stirring 1 time in every 3 ~ 4 hours.Full pond song material must thoroughly be stirred one time by each stirring, particularly must stir bottom Quchi, cause local to burn song in order to avoid material adhesion affects ventilation.

Step [8] prebake:

The material kept well is laid in the prebake of prebake pond, and bake out temperature 60 DEG C ~ 90 DEG C, interval is stirred once for 1 ~ 2 hour.Be dried to that to touch material with hand moistening, grab material not conglomeration with hand-tight, do not lump during sth. made by twisting and can prepare to carry out heat sterilization.Detected by sampling, when moisture content is down to 15 ~ 25%, enter subsequent processing.

Step [9] sterilizing:

By the Turnround basket splendid attire after material cleaning and sterilizing after prebake, be transported to sterilization chamber with transfer trailer.

By the material of prebake by logistics corridor, be transported to the dog-house of Micvowave Sterilization machine, drive Micvowave Sterilization machine, adjusting material feeding thickness by material distributing machine is 2cm, open plant capacity 15 ~ 30 arm, working time, 3 ~ 5min carried out sterilizing, directly entered subsequent processing after sterilizing.

Step [10] spreading for cooling:

Directly enter spreading for cooling workshop section after work in-process sterilizing, through spreading for cooling to 40 ~ 50 DEG C, can pre-packaged be carried out.

Positively effect of the present invention: Gu pigment red colouring agent for food, also used as a Chinese medicine of the present invention adopts liquid-cultivation and fermentation, pigment purity is high, is slightly soluble in water, and it all has good stability to light, heat and oxygen.Wherein containing multiple colour component and functional component, thus there is color development, anticorrosion and nourishing function, be mainly used in the processing of various food, meat product, seasonings painted and anticorrosion, particularly be applied in meat product, the Sudan red, the nitrate that human body are produced to carcinogenesis can be replaced.In meat product application, do not need pigment to be water-soluble, and mostly the insoluble part in pigment red colouring agent for food, also used as a Chinese medicine is starch, all can cooks meat product and fill raw material.Therefore, the pigment red colouring agent for food, also used as a Chinese medicine that the present invention produces be suitable for very much meat product, various food painted.

Accompanying drawing explanation

Fig. 1 is the technological process of production schematic diagram of High color values monascorubin of the present invention.

Embodiment

Below in conjunction with accompanying drawing to a preferred embodiment of the present invention will be described in detail.

The present embodiment provides a kind of High color values red colouring agent for food, also used as a Chinese medicine bacterial classification, and this bacterial classification is preserved in Wuhan, Hubei loujia hill belongs Wuhan University China typical culture collection center on September 17th, 2014, and preserving number is: CCTCCNO:M2014420.

As shown in Figure 1, the production technique of High color values red colouring agent for food, also used as a Chinese medicine bacterial classification described in the present embodiment, comprises the following steps:

S1, vacuum freezing pipe: the preservation in 4 DEG C of refrigerators of vacuum freezing pipe.

Prepared by S2, test tube slant bacterial classification

(improvement czapek's solution is adopted through test tube slant bacterial classification, cultivate 6 ~ 8 days for 32 DEG C ± 2 DEG C) → triangle shake-flask seed (employing glucose two substratum, cultivate 48 ~ 72 hours for 32 DEG C ± 2 DEG C) → seeding tank seed (employing ground rice substratum, 32 DEG C ~ 40 DEG C, air flow 7 ~ 14m3/h, cultivate 24 ~ 48 hours) enlarged culturing step by step obtain production second class inoculum, in culturing process, need sterile sampling to monitor growth situation, determine bacterial classification maturation time.Liquid seeds needs sterile sampling after cultivating maturation before entering subsequent processing, assessment seed liquor quality condition.

Production second class inoculum specification of quality: seed liquor presents pink to red-purple, comparatively thickness, and mycelium pellet is more, even in paper paddle, free from extraneous odour; Microscopy mycelium is sturdy, even, tabula is clear, and spore (pyriform or spherical) is more, without miscellaneous bacteria; PH value lower than 7, generally 6.0 ~ 6.5.Through streak culture, except the colonies typical of object bacteria, grow without other miscellaneous bacterias the purity verifying production bacterial classification.

Through the bacterial classification be up to the standards, can put into production use.

Immersion, the boiling of S3, rice and the rice that dries in the air

Rice is placed in puffed wheat device, soaks.

The puffed wheat time: annual May ~ September puffed wheat 8 ~ 10h, October ~ April next year puffed wheat 10 ~ 12h.

Amount of water requires that the water surface did not have material 5 ~ 10 centimetres.

Pour soaked rice into meal braizing machine, charging capacity 300kg/ pot, require that the rice amount dropping into meal braizing machine is no more than meal braizing machine outer.Open steam, after vapour on material, start timing, the 15 minutes vapour time in maintenance.

Ripe rice specification of quality after steaming: well-done without the white heart, soft not rotten not conglomeration, moisture content 32% ~ 35%.

Dry in the air rice: environmentally temperature grasps the temperature of material flexibly, and when envrionment temperature is lower than 20 DEG C, before inoculation, temperature of charge can be controlled between 38 DEG C ~ 40 DEG C; When envrionment temperature is higher than 20 DEG C, before inoculation, temperature of charge can be controlled between 35 DEG C ~ 38 DEG C.The rice dried well is input to inoculation device.

S4, seed dressing

Inoculate pipeline with steam sterilizing, in secondary seed solution, add food grade lactic acid or food grade Glacial acetic acid that weight percent is 0.1% of rice raw material charging capacity, fully mix, secondary seed solution is introduced inoculation device for subsequent use;

Open inoculation device, regulate seed liquor flow, ensure the homogeneity of inoculum size 6 ~ 8% and the rear material of inoculation, the temperature control of inoculum item is at 32 ~ 35 DEG C;

After inoculation, material Turnover Box or sack splendid attire, be transported to Quchi (temperature is higher than 20 DEG C) or high hot house (temperature is lower than 20 DEG C) with transport pushing cart.

S5, Da Dui

Pile up in time in Quchi or high hot house by material after inoculation, every pond charging capacity 750 ~ 800kg, the side's of scrabbling heap, piles thick about 40 ~ 50cm, observes at any time and records windrow temperature variations.When beating stacking yard item temperature and reaching 45 DEG C, next process can be entered.

Under normal circumstances, windrow temperature rose at 15 ~ 24 hours and requires product temperature (45 DEG C).

S6, stand heap are grown flower

When beating stacking yard item temperature and reaching 45 DEG C, or high hot house can be broken heap material and proceed to fermentation vat and carry out stand heap and grow flower at Quchi.Product temperature when maintenance is grown flower, at 35 ~ 42 DEG C, depending on material product temperature rising speed, is successively made thinner, until full full pond, stand.Behind full full pond, stand, take counter-measure in time depending on the particular case of growing flower, control material product temperature at 35 ~ 38 DEG C, ensure at interval of 4 hours thorough stirrings once.

S7, fermentation culture

One, add water operation and requirement

1, Jia Yishui

Add water the time: generally 24 hours after the heap of stand, grow flower neat.Organoleptic requirements: white hypha is evenly infiltrated in 90% grain of rice surface, and the part grain of rice reddens.Now monascus high-speed rapid growth, thermal value is comparatively large, need repeatedly to add a water on a small quantity, and amount of water is larger.

Water feeding method: while add water, limit stirring, loose depending on bent material, dry wet situation, repeats above-mentioned steps, until material is evenly moisture after adding water.

Control overflow:

1) after adding water 1 hour, on moistening, the Quchi gauze of material and material surface is without free-water.Moisture is (4 ~ October, moisture can remain between 43% ~ 48%, and March November to next year, moisture can remain between 40% ~ 45%) between 40% ~ 48%.

2) control product temperature 33 DEG C ~ 35 DEG C after a water is added.

2, Jia Ershui

Add water the time: the growing state depending on material determines to add the time of two water, after a water 10 hours.

Water feeding method: while add water, limit stirring, loose depending on bent material, dry wet situation, repeats above-mentioned steps, until material is evenly moisture after adding water.

Control overflow: moisture controlled is 43 ~ 50%, and product temperature control at about 33 DEG C, and notices that this stage low temperature must not lower than 30 DEG C, and high temperature must not more than 34 DEG C.

3, culturing process adds water the intermediary and later stages

Intermediary and later stages culturing process water feeding method and requirement are with two water;

4, go out pond to dry and add water the day before yesterday

Go out pond whether to add water and according to water content detection result on the same day, if moisture is more than 43%, then need not will add water the day before yesterday, otherwise suitably moisturizing, after moisturizing, moisture requirement is no more than 48%, and product temperature control is at about 33 DEG C, and low temperature must not lower than 30 DEG C, and high temperature must not more than 34 DEG C.

Two, stirring operation and requirement

Within after adding water about one hour, require again that thoroughly stirring once.Stirring afterwards will depending on the growing state of material, but must ensure to want stirring 1 time in every 3 ~ 4 hours.Full pond song material must thoroughly be stirred one time by each stirring, particularly must stir bottom Quchi, cause local to burn song in order to avoid material adhesion affects ventilation.

S8, oven dry.

S9, pulverizing.

S10, inspection.

S11, packaging.

S12, finished product are put in storage.

Positively effect of the present invention: 1) High color values red colouring agent for food, also used as a Chinese medicine is with Monascus color aspergillus (Monascusanka) for starting strain, by multi-cultur es assortment of genes technology, filters out the excellent species of High color values.

2) pigment purity is high, and it all has good stability to light, heat and oxygen.Therefore, pigment red colouring agent for food, also used as a Chinese medicine be suitable for very much meat product, various food painted.

3) High color values red colouring agent for food, also used as a Chinese medicine bacterial classification has stronger product look ability, during the fermentation, produces haematochrome and yellow pigment, improves Red Index and the yellowness index of product, makes that product color is bright-coloured, glow.

4) wherein containing multiple colour component and functional component, thus there is color development, anticorrosion and nourishing function, be mainly used in the processing of various food, meat product, seasonings painted and anticorrosion, particularly be applied in meat product, the Sudan red, the nitrate that human body are produced to carcinogenesis can be replaced.In meat product application, do not need pigment to be water-soluble, and mostly the insoluble part in pigment red colouring agent for food, also used as a Chinese medicine is starch, all can cooks meat product and fill raw material.

5) High color values red colouring agent for food, also used as a Chinese medicine bacterial classification can resist higher osmotic pressure, can in pH value less than 7.0, tolerable temperature 48 DEG C, optimum temperuture 30 DEG C ~ 35 DEG C, and salinity 22 ° of below B é use.

Above-describedly be only the preferred embodiments of the present invention; be understood that; the explanation of above embodiment just understands method of the present invention and core concept thereof for helping; the protection domain be not intended to limit the present invention; all any amendments, equivalent replacement etc. made within thought of the present invention and principle, all should be included within protection scope of the present invention.

Claims (2)

1. a High color values monascorubin bacterial classification, is characterized in that: this bacterial classification is preserved in China typical culture collection center on September 17th, 2014, and deposit number is: CCTCCNO:M2014420.

2. a production technique for High color values monascorubin bacterial classification described in claim 1, is characterized in that: comprise the following steps:

The preservation in 4 DEG C of refrigerators of step [1] vacuum freezing pipe;

Step [2] strain expanded culture:

Test tube slant bacterial classification: adopt improvement czapek's solution, cultivate 6 ~ 8 days → triangle shake-flask seed for 32 DEG C ± 2 DEG C: adopt glucose two substratum, cultivate 48 ~ 72 hours → seeding tank seed for 32 DEG C ± 2 DEG C: adopt ground rice substratum, 32 DEG C ~ 40 DEG C, air flow 7 ~ 14m ³/ h, cultivates 24 ~ 48 hours; Step through above-mentioned enlarged culturing step by step obtains production second class inoculum, in culturing process, need sterile sampling to monitor growth situation, determine bacterial classification maturation time, after liquid seeds cultivates maturation, sterile sampling is needed, assessment seed liquor quality condition before entering subsequent processing;

Production second class inoculum specification of quality: seed liquor presents pink to red-purple, comparatively thickness, and mycelium pellet is more, even in paper paddle, free from extraneous odour; Microscopy mycelium is sturdy, even, tabula is clear, and spore (pyriform or spherical) is more, without miscellaneous bacteria; PH value lower than 7, generally 6.0 ~ 6.5.Through streak culture, except the colonies typical of object bacteria, grow without other miscellaneous bacterias the purity verifying production bacterial classification; Through the bacterial classification be up to the standards, can put into production use.

Step [3]

Immersion, the boiling of rice and the rice that dries in the air

Rice is placed in puffed wheat device, soaks;

The puffed wheat time: annual May ~ September puffed wheat 8 ~ 10h, October ~ April next year puffed wheat 10 ~ 12h.

Amount of water requires that the water surface did not have material 5 ~ 10 centimetres;

Pour soaked rice into meal braizing machine, charging capacity 300kg/ pot, require that the rice amount dropping into meal braizing machine is no more than meal braizing machine outer.Open steam, after vapour on material, start timing, the 15 minutes vapour time in maintenance;

Ripe rice specification of quality after steaming: well-done without the white heart, soft not rotten not conglomeration, moisture content 32% ~ 35%.

Dry in the air rice: environmentally temperature grasps the temperature of material flexibly, and when envrionment temperature is lower than 20 DEG C, before inoculation, temperature of charge can be controlled between 38 DEG C ~ 40 DEG C; When envrionment temperature is higher than 20 DEG C, before inoculation, temperature of charge can be controlled between 35 DEG C ~ 38 DEG C.The rice dried well is input to inoculation device.

Step [4] is dressed seed:

Inoculate pipeline with steam sterilizing, in secondary seed solution, add food grade lactic acid or food grade Glacial acetic acid that weight percent is 0.1% of rice raw material charging capacity, fully mix, secondary seed solution is introduced inoculation device for subsequent use;

Open inoculation device, regulate seed liquor flow, ensure the homogeneity of inoculum size 6 ~ 8% and the rear material of inoculation, the temperature control of inoculum item is at 32 ~ 35 DEG C;

After inoculation, material Turnover Box or sack splendid attire, be transported to Quchi (temperature is higher than 20 DEG C) or high hot house (temperature is lower than 20 DEG C) with transport pushing cart.

Step [5] beats heap:

Pile up in time in Quchi or high hot house by material after inoculation, every pond charging capacity 750 ~ 800kg, the side's of scrabbling heap, piles thick about 40 ~ 50cm, observes at any time and records windrow temperature variations, when beating stacking yard item temperature and reaching 45 DEG C, can enter next process.

Step 6] stand heap grow flower:

When beating stacking yard item temperature and reaching 45 DEG C, or high hot house can be broken heap material and proceed to fermentation vat and carry out stand heap and grow flower at Quchi, product temperature when maintenance is grown flower is at 35 ~ 42 DEG C, depending on material product temperature rising speed, successively make thinner, until full full pond, stand, behind full full pond, stand, particular case depending on growing flower takes counter-measure in time, controls material product temperature at 35 ~ 38 DEG C, ensures at interval of 4 hours thorough stirrings once.

Step [7] fermentation culture:

7a. adds water and operates and requirement

1. a water is added

Add water the time: generally 24 hours after the heap of stand, grow flower neat; Organoleptic requirements: white hypha is evenly infiltrated in 90% grain of rice surface, and the part grain of rice reddens; Now monascus high-speed rapid growth, thermal value is comparatively large, need repeatedly to add a water on a small quantity, and amount of water is larger.

Water feeding method: while add water, limit stirring, loose depending on bent material, dry wet situation, repeats above-mentioned steps, until material is evenly moisture after adding water;

Control overflow:

After adding water 1 hour, on moistening, the Quchi gauze of material and material surface is without free-water.Moisture is (4 ~ October, moisture can remain between 43% ~ 48%, and March November to next year, moisture can remain between 40% ~ 45%) between 40% ~ 48%;

Add control product temperature 33 DEG C ~ 35 DEG C after a water.

2. two water are added

Add water the time: the growing state depending on material determines to add the time of two water, after a water 10 hours.

Water feeding method: while add water, limit stirring, loose depending on bent material, dry wet situation, repeats above-mentioned steps, until material is evenly moisture after adding water.

Control overflow: moisture controlled is 43 ~ 50%, and product temperature control at about 33 DEG C, and notices that this stage low temperature must not lower than 30 DEG C, and high temperature must not more than 34 DEG C.

3. culturing process adds water the intermediary and later stages

Intermediary and later stages culturing process water feeding method and requirement are with two water;

4. go out pond to dry and add water the day before yesterday

Go out pond whether to add water and according to water content detection result on the same day, if moisture is more than 43%, then need not will add water the day before yesterday, otherwise suitably moisturizing, after moisturizing, moisture requirement is no more than 48%, and product temperature control is at about 33 DEG C, and low temperature must not lower than 30 DEG C, and high temperature must not more than 34 DEG C.

The operation of 7b. stirring and requirement

Within after adding water about one hour, require again that thoroughly stirring once.Stirring afterwards will depending on the growing state of material, but must ensure to want stirring 1 time in every 3 ~ 4 hours.Full pond song material must thoroughly be stirred one time by each stirring, particularly must stir bottom Quchi, cause local to burn song in order to avoid material adhesion affects ventilation.

Step [8] prebake:

The material kept well is laid in the prebake of prebake pond, and bake out temperature 60 DEG C ~ 90 DEG C, interval is stirred once for 1 ~ 2 hour.Be dried to that to touch material with hand moistening, grab material not conglomeration with hand-tight, do not lump during sth. made by twisting and can prepare to carry out heat sterilization; Detected by sampling, when moisture content is down to 15 ~ 25%, enter subsequent processing;

Step [9] sterilizing:

By the Turnround basket splendid attire after material cleaning and sterilizing after prebake, be transported to sterilization chamber with transfer trailer; By the material of prebake by logistics corridor, be transported to the dog-house of Micvowave Sterilization machine, drive Micvowave Sterilization machine, adjusting material feeding thickness by material distributing machine is 2cm, open plant capacity 15 ~ 30 arm, working time, 3 ~ 5min carried out sterilizing, directly entered subsequent processing after sterilizing;

Step [10] spreading for cooling:

Directly enter spreading for cooling workshop section after work in-process sterilizing, through spreading for cooling to 40 ~ 50 DEG C, can pre-packaged be carried out.