CN105054124A - Fermentation type semi-dried red yeast fish and processing method and application thereof - Google Patents
Fermentation type semi-dried red yeast fish and processing method and application thereof Download PDFInfo
- Publication number
- CN105054124A CN105054124A CN201510541249.8A CN201510541249A CN105054124A CN 105054124 A CN105054124 A CN 105054124A CN 201510541249 A CN201510541249 A CN 201510541249A CN 105054124 A CN105054124 A CN 105054124A
- Authority
- CN
- China
- Prior art keywords
- fish
- monascus
- dried red
- red wine
- dried
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 91
- 238000000855 fermentation Methods 0.000 title claims abstract description 67
- 230000004151 fermentation Effects 0.000 title claims abstract description 67
- 238000003672 processing method Methods 0.000 title claims abstract description 19
- 229940057059 Monascus purpureus Drugs 0.000 title abstract 6
- 239000011780 sodium chloride Substances 0.000 claims abstract description 61
- 235000013305 food Nutrition 0.000 claims abstract description 19
- 238000002360 preparation method Methods 0.000 claims abstract description 17
- 241000228347 Monascus <ascomycete fungus> Species 0.000 claims description 94
- 150000003839 salts Chemical class 0.000 claims description 60
- 235000020095 red wine Nutrition 0.000 claims description 48
- 239000003795 chemical substances by application Substances 0.000 claims description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 238000005554 pickling Methods 0.000 claims description 20
- 239000002609 media Substances 0.000 claims description 18
- 229920001690 polydopamine Polymers 0.000 claims description 18
- 210000004215 spores Anatomy 0.000 claims description 18
- 238000004140 cleaning Methods 0.000 claims description 15
- 235000007164 Oryza sativa Nutrition 0.000 claims description 14
- 235000020995 raw meat Nutrition 0.000 claims description 14
- 235000009566 rice Nutrition 0.000 claims description 14
- 239000000725 suspension Substances 0.000 claims description 12
- 239000001963 growth media Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 239000012266 salt solution Substances 0.000 claims description 9
- 238000011081 inoculation Methods 0.000 claims description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L mgso4 Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- 235000021110 pickles Nutrition 0.000 claims description 7
- 230000004913 activation Effects 0.000 claims description 6
- 239000011248 coating agent Substances 0.000 claims description 6
- 238000000576 coating method Methods 0.000 claims description 6
- 230000035800 maturation Effects 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M Monopotassium phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 4
- 108010073771 Soybean Proteins Proteins 0.000 claims description 4
- 238000005516 engineering process Methods 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 4
- 235000019710 soybean protein Nutrition 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims 2
- 238000001035 drying Methods 0.000 abstract description 20
- 239000000796 flavoring agent Substances 0.000 abstract description 16
- 235000019634 flavors Nutrition 0.000 abstract description 15
- 235000013527 bean curd Nutrition 0.000 abstract description 9
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 230000014860 sensory perception of taste Effects 0.000 abstract 2
- 235000019640 taste Nutrition 0.000 abstract 2
- 230000035917 taste Effects 0.000 abstract 2
- 240000006149 Monascus purpureus Species 0.000 abstract 1
- 235000002322 Monascus purpureus Nutrition 0.000 abstract 1
- 235000010855 food raising agent Nutrition 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 235000019688 fish Nutrition 0.000 description 66
- 241000276701 Oreochromis mossambicus Species 0.000 description 39
- 241001275898 Mylopharyngodon piceus Species 0.000 description 13
- 241000209094 Oryza Species 0.000 description 12
- 238000004040 coloring Methods 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 10
- 235000013339 cereals Nutrition 0.000 description 9
- 239000003814 drug Substances 0.000 description 9
- 229920003023 plastic Polymers 0.000 description 9
- 238000003756 stirring Methods 0.000 description 8
- 210000001015 Abdomen Anatomy 0.000 description 6
- 241000723353 Chrysanthemum Species 0.000 description 6
- 235000007516 Chrysanthemum Nutrition 0.000 description 6
- 235000005986 Chrysanthemum x morifolium Nutrition 0.000 description 6
- 241001596950 Larimichthys crocea Species 0.000 description 6
- 238000005520 cutting process Methods 0.000 description 6
- 235000015169 fillet of fish Nutrition 0.000 description 6
- 210000000056 organs Anatomy 0.000 description 6
- 206010036590 Premature baby Diseases 0.000 description 5
- 238000007605 air drying Methods 0.000 description 5
- 238000011068 load Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 235000015598 salt intake Nutrition 0.000 description 5
- 230000035943 smell Effects 0.000 description 5
- 229920002472 Starch Polymers 0.000 description 4
- 210000004243 Sweat Anatomy 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 235000020127 ayran Nutrition 0.000 description 4
- 239000003205 fragrance Substances 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 235000019698 starch Nutrition 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 235000019542 Cured Meats Nutrition 0.000 description 3
- 241000276707 Tilapia Species 0.000 description 3
- 239000011149 active material Substances 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000252203 Clupea harengus Species 0.000 description 2
- 241000252230 Ctenopharyngodon idella Species 0.000 description 2
- 241000252233 Cyprinus carpio Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241000252234 Hypophthalmichthys nobilis Species 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 240000001016 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000000844 anti-bacterial Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 235000019514 herring Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 230000000050 nutritive Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N oxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 235000012015 potatoes Nutrition 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 210000004369 Blood Anatomy 0.000 description 1
- DNVPQKQSNYMLRS-APGDWVJJSA-N Ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@@H](CC[C@@]3([C@@H]([C@H](C)/C=C/[C@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-APGDWVJJSA-N 0.000 description 1
- DNVPQKQSNYMLRS-LNHMRCHQSA-N Ergosterol Natural products O[C@@H]1CC=2[C@@](C)([C@@H]3C([C@H]4[C@@](C)([C@H]([C@@H](/C=C/[C@H](C(C)C)C)C)CC4)CC3)=CC=2)CC1 DNVPQKQSNYMLRS-LNHMRCHQSA-N 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000002959 anti-hypotensive Effects 0.000 description 1
- 230000003078 antioxidant Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000001580 bacterial Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 235000002864 food coloring agent Nutrition 0.000 description 1
- 230000001077 hypotensive Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006011 modification reaction Methods 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 230000001954 sterilising Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Abstract
The present invention belongs to the technical field of food processing and more particularly relates to a fermentation type semi-dried red yeast fish and a processing method and an application thereof. The fermentation type semi-dried red yeast fish processing method includes the following steps: (1) preparation of low-salt fish blocks; (2) preparation of monascus purpureus leavening agent; (3) inoculating and fermenting; and (4) drying. The preparedfermentation type semi-dried red yeast fish is ruddy in color, richer in nutrition, and mellow in taste, and has an outstanding flavor of fermented red beancurd. Compared with the ordinary semi-dried fish, the fermentation type semi-dried red yeast fish tastes good and is higher in product nutrition value. Besides, the preparation method is simple, low in cost, and suitable for industrial production and promotion.
Description
Technical field
The invention belongs to food processing technology field, particularly the half-dried red wine dregs fish of a kind of fermented type and processing method thereof and application.
Background technology
Tradition dried fish mainly adopts high salt Exposure to Sunlight technique to produce, and the salinity of product is higher and moisture is lower, and mouthfeel is relatively poor.Along with popularizing of cold chain, occurred relying on cold chain to carry out the half-dried fish of accumulating and sale, mouthfeel is relatively better, only need cook i.e. edible, be subject to consumers welcomed.But because the drying time of half-dried fish is short, lack the natural inoculation fermentation of microorganism in dry run, cause the local flavor of product single, lose the cured Flavor of traditional dried fish, color does not have traditional dried fish ruddy yet.
Fermentation Condition of Monascus spp not only can produce natural red colouring matter, improves the color of food, but also can give the local flavor of food uniqueness while improving food color, is used widely in the food industry.In recent years, people isolate various active material from red colouring agent for food, also used as a Chinese medicine, and these active materials not only contribute to reducing blood lipid, hypotensive, also can be antibacterial anticorrosion, are applied in food and can extend preservation term.
Summary of the invention
In order to overcome the shortcoming of prior art with not enough, primary and foremost purpose of the present invention is the processing method providing the half-dried red wine dregs fish of a kind of fermented type, this processing method adopts Fermentation Condition of Monascus spp, prepare the half-dried red wine dregs fish of fermented type by manual control fermentation's condition, its production technology is simple, quality easily controls, safety and sanitation; This processing method both can be applied to the fresh-water fishes such as Tilapia mossambica, black carp, grass carp, silver carp, carp, and also can be applied to the processing of the fish such as yellow croaker, Chinese herring, social and economic benefits is remarkable.
The half-dried red wine dregs fish of the fermented type that another object of the present invention is to provide above-mentioned processing method to prepare, this fermented type half-dried red wine dregs fish is nutritious, and flavour is mellow, has outstanding ferment local-flavor.
Another object of the present invention is the application providing the half-dried red wine dregs fish of above-mentioned fermented type.
Object of the present invention is achieved through the following technical solutions:
A processing method for the half-dried red wine dregs fish of fermented type, comprises the steps:
(1) preparation of less salt fish block
Raw meat being removed through the salt solution cleaning of pretreated fish, then by going the fish after raw meat to pickle with salt, obtaining less salt fish;
(2) preparation of Fermentation Condition of Monascus spp agent
Monascus strain is activated, and prepares Monascus spore suspension, cultivate in the Monascus spore suspension prepared access liquid seed culture medium, obtain monascus seed liquor; Then cultivate 1 ~ 3 day for 25 ~ 35 DEG C after rice, water, monascus seed liquor being mixed according to the ratio of mass ratio 70: 15: 15, obtain Fermentation Condition of Monascus spp agent (prematurity red wine dregs);
(3) inoculation fermentation
The Fermentation Condition of Monascus spp agent prepare step (2) and water, according to the mixed also homogenate of the ratio of mass ratio (1: 1) ~ (5: 1), obtain Fermentation Condition of Monascus spp agent homogenate; The surface uniform coating Fermentation Condition of Monascus spp agent homogenate of the less salt fish then prepared in step (1), in 25 ~ 35 DEG C of bottom fermentation 8 ~ 16h;
(4) dry
By dry for the less salt dried fish fermented in step (3), obtain the half-dried red wine dregs fish of fermented type;
Fish described in step (1) is preferably at least one in Tilapia mossambica, black carp, grass carp, silver carp, carp, yellow croaker and Chinese herring;
Fish is preferably removed internal organ and squama by the pretreated concrete operations described in step (1), cuts open (not cutting off), opens, flatten along back or belly, is formed except the nearly symmetrical fillet of fish tail partial circumscription, and cleans;
The mass fraction of the salt solution described in step (1) is 1% ~ 4% (w/w);
The consumption of the salt described in step (1) is 3% ~ 8% (w/w) of fish quality;
The temperature of pickling described in step (1) is 4 DEG C ~ 10 DEG C, and the time of pickling is 1h ~ 5h;
Monascus strain described in step (2) is preferably GIM3.445 or GIM3.439;
The concrete steps of the monascus strain activation described in step (2) are preferably: be inoculated into by monascus strain on PDA slant medium, cultivate 3 ~ 5 days, until the Monascus spore maturation on PDA slant medium becomes bronzing for 30 ~ 35 DEG C;
The formula of described PDA slant medium is: peeled potatoes 20% (w/w), glucose 2% (w/w), agar 1.5% (w/w), and water supplies 100% (w/w);
The formula of the liquid seed culture medium described in step (2) is: ground rice 6.5% (w/w), magnesium sulfate 0.05% (w/w), potassium dihydrogen phosphate 0.1% (w/w), soybean protein 1.2% (w/w), water supply 100%;
The condition of culture of the monascus seed liquor described in step (2) is preferably: under the condition of rotating speed 150 ~ 200r/min, temperature 25 ~ 35 DEG C, cultivate 3 ~ 5 days;
The mass ratio of the less salt fish described in step (3) and Fermentation Condition of Monascus spp agent homogenate is preferably (5: 1) ~ (9: 1);
The condition of the drying described in step (4) is preferably hot pump in low temp drying;
Condition more preferably 25 ~ 35 DEG C of drying 10 ~ 20h of the drying described in step (4);
The half-dried red wine dregs fish of a kind of fermented type, is prepared by above-mentioned processing method;
The salt content of the half-dried red wine dregs fish of described fermented type is 2% ~ 6% (w/w), and moisture wet basis is 30% ~ 55% (w/w);
The salt content of the half-dried red wine dregs fish of described fermented type is preferably 4.5% (w/w), and moisture wet basis is preferably 48% (w/w);
Described fermented type half-dried red wine dregs fish color is reddish tan, has obvious fermented red beancurd characteristic flavor on basis, and under 0 DEG C ~ 10 DEG C conditions, storage life can reach more than three months;
The half-dried red wine dregs fish of described fermented type directly can be packaged into finished product, also can be processed into can and inner wrapping foods packed in carton containers goods etc. further;
Principle of the present invention is different from traditional aid bag: traditional aid bag adopts the vinasse of fermenting-ripening to prepare, because the starch major part in the vinasse of fermenting-ripening transfers carbohydrate to, viscosity declines, vinasse cannot be hung and stick with paste on fish body, prepare in aid bag process and need fish to be immersed in completely in vinasse, cost is high; And ethanol content is higher in mature alcohol distillery grain, strain aging, vigor are low, inhibit the fermentation of fish.The present invention adopts prematurity red colouring agent for food, also used as a Chinese medicine grain to replace ripe vinasse to overcome the problems referred to above: first, still remaining in immature red colouring agent for food, also used as a Chinese medicine grain have a large amount of starch, and these starch can be used for the fermentation of red wine dregs fish, without the need to adding carbohydrate in addition again in sweat; The second, immature red colouring agent for food, also used as a Chinese medicine grain viscosity is higher, is easy to hang at fish body stick with paste, and only need be coated on fish surface by after the homogenate of immature red colouring agent for food, also used as a Chinese medicine grain, reduce costs, simplify the operation; 3rd, compare with ripe red colouring agent for food, also used as a Chinese medicine grain, the bacterial classification of prematurity red colouring agent for food, also used as a Chinese medicine grain enlivens, and after mixing with fish body, fermentation can be made more abundant; 4th, the present invention adopts prematurity red colouring agent for food, also used as a Chinese medicine grain to ferment, and the monascorubin produced in sweat not only makes fermented type half-dried red wine dregs fish color ruddy, possess better outward appearance, and product has special fermented red beancurd fragrance; 5th, monascus energy for growth is strong, significantly can suppress the growth of other miscellaneous bacterias in fermented type half-dried red wine dregs fish preparation process, extends the storage life of product.The more important thing is, monascus can produce large number of biological active material during the fermentation: monascorubin, MonacolinK, ergosterol etc., has the physiologically actives such as anti-oxidant, hypotensive and enhanced machine body immunity function.
The present invention compared with prior art, has following advantage and beneficial effect:
(1) salt dried fish high with tradition compares, and the fermented type half-dried red wine dregs fish salt content of the present invention's processing is lower than traditional dried fish, and water content is higher than traditional dried fish, and color is ruddy, is of high nutritive value, and mouthfeel is better than traditional dried fish.
(2) compare with common half-dried fish, the more common half-dried fish of fermented type half-dried red wine dregs fish of the present invention's processing (after pickling, prosthetic inoculation fermentation process, convection drying becomes leather hard) color is ruddy, mouthfeel good, have outstanding fermented red beancurd local flavor, the nutritive value of product is higher.
(3) the present invention adopts the half-dried fish after fermentation of prematurity red colouring agent for food, also used as a Chinese medicine grain coating, do not need to add water and starch, also jolting is not needed, whole sweat oxygen supply is sufficient, fermentation is quick and abundant, in product water-solubility protein and flavor substance intact, fragrance and the flavour of product are significantly better than existing product.
(4) the present invention instead of biologic product-peptone with soybean protein in the formula of seed culture medium, and meet the requirement of people to raw-food material, consumer can accept.Directly can add with monascus seed liquor in the formula of Fermentation Condition of Monascus spp agent, and no longer need the mycelia of isolating monascus seed liquor, drastically increase the utilization rate of monascus seed liquor, improve fermentation efficiency, simplify the operation step, saves cost.
Accompanying drawing explanation
Fig. 1 is the process chart of the half-dried red wine dregs Tilapia mossambica of fermented type prepared by embodiment 1.
Fig. 2 is fermented type half-dried red wine dregs Tilapia mossambica product form figure prepared by embodiment 1.
Fig. 3 is common half-dried Tilapia mossambica product form figure prepared by comparative example 1.
Fig. 4 be comparative example 2 prepare Fermentation Condition of Monascus spp Tilapia mossambica product form figure.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
PDA slant medium formula used is: peeled potatoes 20% (w/w), glucose 2% (w/w), agar 1.5% (w/w), and water supplies 100% (w/w);
Liquid seed culture medium formula used is: ground rice 6.5% (w/w), magnesium sulfate 0.05% (w/w), potassium dihydrogen phosphate 0.1% (w/w), soybean protein 1.2% (w/w), water supply 100%;
GIM3.445 is purchased from Guangdong Province's Culture Collection;
GIM3.439 is purchased from Guangdong Province's Culture Collection;
Embodiment 1
(1) Tilapia mossambica is removed internal organ and squama, cut open along back or belly (not cutting off), open, flatten, formed except the nearly symmetrical fillet of fish tail partial circumscription, clean with clear water, remove raw meat with salt solution (salt preparation) cleaning of 4% (w/w), raw meat Tilapia Fillet salt then will be gone to pickle, wherein, the temperature of pickling is 10 DEG C, and the time of pickling is 1h, and salt consumption is 8% (w/w) of fish quality; Drain after pickling, stripping and slicing, obtain less salt Tilapia mossambica block;
(2) preparation of Fermentation Condition of Monascus spp agent
1. monascus specie activation
Monascus strain GIM3.445 is inoculated into PDA slant medium, and in 30 DEG C of insulating boxs, activate 3 days, the Monascus spore maturation observed on PDA slant medium becomes bronzing;
2. monascus specie spreads cultivation
Sterilized water is poured in PDA slant medium, jolt 15min, obtain Monascus spore suspension, Monascus spore suspension is washed in triangular flask, add liquid seed culture medium, put into shaking table, rotating speed be 150r/min, under temperature is the condition of 35 DEG C, cultivate 3d, obtain monascus seed liquor;
3. Fermentation Condition of Monascus spp agent is prepared
By rice and water according to 10: 9 ratio, water proof boiling 40min, is cooled to room temperature, then by rice, sterilized water, monascus seed liquor according to after the ratio mixing and stirring of mass ratio 70: 15: 15, build to be placed in 35 DEG C of incubators with gauze and cultivate 1 day;
(3) inoculation fermentation
The Fermentation Condition of Monascus spp agent prepare step (2) and distilled water mix homogenate according to the ratio of mass ratio 5: 1, obtain Fermentation Condition of Monascus spp agent homogenate; In the less salt Tilapia mossambica block surface uniform coating Fermentation Condition of Monascus spp agent homogenate that step (1) prepares, wherein, the mass ratio of less salt Tilapia mossambica block and Fermentation Condition of Monascus spp agent homogenate is 5: 1; Then the less salt Tilapia mossambica block after smearing is placed in clean basin, with gauze sealing, in room temperature 35 DEG C of bottom fermentation 10h;
(4) dry
Entirely be spread out on plastic processing frame by the less salt Tilapia mossambica block fermented in step (3), be placed in hot pump in low temp drying box and carry out cold air drying, wherein, baking temperature is 35 DEG C, and drying time is 10h, in drying course, often stir fish body; Obtain the half-dried red wine dregs Tilapia mossambica (Fig. 1) of fermented type, the salt content of the half-dried red wine dregs Tilapia mossambica of this fermented type is 5% (w/w), and moisture wet basis is 48% (w/w), and color is reddish tan, meat is glossy, has obvious fermented red beancurd local flavor and typical wind cured meat and fish.
(5) the half-dried red wine dregs Tilapia mossambica of fermented type step (4) prepared puts into die pallet, and load transparent plastic bag, vacuumize, obtain salt content and be about 5%, water content is about the finished product of 48%.
Embodiment 2
(1) yellow croaker is removed internal organ and squama, cut open along back or belly (not cutting off), open, flatten, formed except the nearly symmetrical fillet of fish tail partial circumscription, clean with clear water, remove raw meat with salt solution (salt preparation) cleaning of 1% (w/w), raw meat chrysanthemum fillet salt then will be gone to pickle, wherein, the temperature of pickling is 4 DEG C, and the time of pickling is 5h, and salt consumption is 3% (w/w) of fish weight; Drain after pickling, obtain less salt chrysanthemum fillet;
(2) preparation of Fermentation Condition of Monascus spp agent
1. monascus specie activation
Monascus strain GIM3.439 is inoculated into PDA slant medium, and in 35 DEG C of insulating boxs, activate 4 days, the Monascus spore maturation observed on PDA slant medium becomes bronzing;
2. monascus specie spreads cultivation
Sterilized water is poured in PDA slant medium, jolt 15min, obtain Monascus spore suspension, Monascus spore suspension is washed in triangular flask, add liquid seed culture medium, put into shaking table, rotating speed be 150r/min, under temperature is the condition of 25 DEG C, cultivate 5 days, obtain monascus seed liquor;
3. Fermentation Condition of Monascus spp agent is prepared
By rice and the water ratio according to 10:9, water proof boiling 40min, is cooled to room temperature, then by rice, sterilized water, monascus seed liquor according to after the ratio mixing and stirring of mass ratio 70: 15: 15, build to be placed in 25 DEG C of incubators with gauze and cultivate 3 days;
(3) inoculation fermentation
The Fermentation Condition of Monascus spp agent prepare step (2) and distilled water mix homogenate according to the ratio of mass ratio 3: 1, obtain Fermentation Condition of Monascus spp agent homogenate; In the less salt chrysanthemum fillet surface uniform coating Fermentation Condition of Monascus spp agent homogenate that step (1) prepares, wherein, the mass ratio of less salt chrysanthemum fillet and Fermentation Condition of Monascus spp agent homogenate is 9: 1; Then the less salt chrysanthemum fillet after smearing are placed in clean basin, with gauze sealing, in 25 DEG C of bottom fermentations 16 hours;
(4) dry
Entirely be spread out on plastic processing frame by the less salt chrysanthemum fillet fermented in step (3), be placed in hot pump in low temp drying box and carry out cold air drying, wherein, baking temperature is 30 DEG C, and drying time is 20h, in dry run, often stir fish body; Obtain the half-dried red wine dregs yellow croaker of fermented type, the salt content of the half-dried red wine dregs yellow croaker of this fermented type is 3.5% (w/w), and moisture wet basis is 30% (w/w), and color is reddish tan, has obvious fermented red beancurd local flavor and typical wind cured meat and fish.
(4) half-dried for fermented type red wine dregs yellow croaker is put into die pallet, load transparent plastic bag, vacuumize, obtain salt content and be about 3.5% (w/w), water content is about the finished product of 30% (w/w).
Embodiment 3
(1) black carp is removed internal organ and squama, cut open along back or belly (not cutting off), open, flatten, formed except the nearly symmetrical fillet of fish tail partial circumscription, clean with clear water, remove raw meat with salt solution (salt preparation) cleaning of 2% (w/w), then raw meat black carp sheet salt will be gone to pickle, wherein, the temperature of pickling is 7 DEG C, and the time of pickling is 3h,, salt consumption is 8% (w/w) of fish quality; Drain after pickling, stripping and slicing, obtain less salt black carp block;
(2) preparation of Fermentation Condition of Monascus spp agent
1. monascus specie activation
Monascus strain GIM3.445 is inoculated into PDA slant medium, and in 33 DEG C of insulating boxs, activate 5 days, the Monascus spore maturation observed on PDA slant medium becomes bronzing;
2. monascus specie spreads cultivation
Sterilized water is poured in PDA slant medium, jolt 15min, obtain Monascus spore suspension, Monascus spore suspension is washed in triangular flask, add liquid seed culture medium, put into shaking table, rotating speed be 150r/min, under temperature is the condition of 30 DEG C, cultivate 4 days, obtain monascus seed liquor;
3. Fermentation Condition of Monascus spp agent is prepared
By rice and water according to 10: 9 ratio, water proof boiling 40min, is cooled to room temperature, then by rice, sterilized water, monascus seed liquor according to after the ratio mixing and stirring of mass ratio 70: 15: 15, build to be placed in 30 DEG C of incubators with gauze and cultivate 2 days;
(3) inoculation fermentation
The Fermentation Condition of Monascus spp agent prepare step (2) and distilled water mix homogenate according to the ratio of mass ratio 1: 1, obtain Fermentation Condition of Monascus spp agent homogenate; In the less salt black carp block surface uniform coating Fermentation Condition of Monascus spp agent homogenate that step (1) prepares, wherein, the mass ratio of less salt black carp block and Fermentation Condition of Monascus spp agent homogenate is 8: 1; Then the less salt black carp block after smearing is placed in clean basin, with gauze sealing, in 30 DEG C of fermentation 12h;
(4) dry
Entirely be spread out on plastic processing frame by the less salt black carp block fermented in step (3), be placed in hot pump in low temp drying box and carry out cold air drying, wherein, baking temperature is 25 DEG C, and drying time is 10h, in dry run, often stir fish body; Obtain the half-dried red wine dregs black carp of fermented type, the salt content of the half-dried red wine dregs black carp of this fermented type is 6% (w/w), and moisture wet basis is 55% (w/w), and color is reddish tan, has obvious fermented red beancurd local flavor and typical wind cured meat and fish.
(5) the further stripping and slicing of fermented type half-dried red wine dregs black carp step (4) prepared, add suitably flavored material, load digestion resistant bag, 121 DEG C of sterilizations, bactericidal formula is 10 minutes-25 minutes-10 minutes, obtain fermented type half-dried red wine dregs black carp foods packed in carton containers, this product salt content is 6% (w/w), and water content is about 55% (w/w), color bronzing, meat is glossy, fresh perfume (or spice) assails the nostrils, and has typical fermented red beancurd fragrance, can preserve 12 months by normal temperature.
Comparative example 1
(1) Tilapia mossambica is removed internal organ and squama, cut open along back or belly (not cutting off), open, flatten, formed except the nearly symmetrical fillet of fish tail partial circumscription, clean with clear water, remove raw meat with salt solution (salt preparation) cleaning of 4% (w/w), raw meat Tilapia Fillet salt then will be gone to pickle, wherein, the temperature of pickling is 10 DEG C, and the time of pickling is 1h, and salt consumption is 8% (w/w) of fish quality; Drain after pickling, stripping and slicing, obtain less salt Tilapia mossambica block;
(2) the less salt Tilapia mossambica block prepared in step (1) is entirely spread out on plastic processing frame, be placed in hot pump in low temp drying box and carry out cold air drying, wherein, temperature is 35 DEG C, drying time is 10h, in drying course, often stir fish body, obtains half-dried Tilapia mossambica block, the salt content of this half-dried Tilapia mossambica block is 5% (w/w), and moisture wet basis is 48% (w/w);
(3) half-dried Tilapia mossambica block is put into die pallet, load transparent plastic bag, vacuumize, obtain salt content and be about 5% (w/w), water content is about the finished product of 48% (w/w).
Comparative example 2 (adopting monascus mycelia, the Monascus fermentation broth fermentation that ground rice and physiological saline are made into)
(1) Tilapia mossambica is removed internal organ and squama, cut open along back or belly (not cutting off), open, flatten, formed except the nearly symmetrical fillet of fish tail partial circumscription, clean with clear water, remove raw meat with salt solution (salt preparation) cleaning of 4% (w/w), raw meat Tilapia Fillet salt then will be gone to pickle, wherein, the temperature of pickling is 10 DEG C, and the time of pickling is 1h, and salt consumption is 8% (w/w) of fish quality; Drain after pickling, stripping and slicing, obtain less salt Tilapia mossambica block;
(2) preparation of Fermentation Condition of Monascus spp agent
1. monascus specie activation
Monascus strain GIM3.445 is inoculated into PDA slant medium, and in 30 DEG C of insulating boxs, activate 3 days, the Monascus spore maturation observed on PDA slant medium becomes bronzing;
2. monascus specie spreads cultivation
Sterilized water is poured in PDA slant medium, jolt 15min, obtain Monascus spore suspension, washed by Monascus spore suspension in triangular flask, (ground rice 6.5% (w/w), magnesium sulfate 0.05% (w/w), potassium dihydrogen phosphate 0.1% (w/w), peptone 2% (w/w), water supply 100% to add liquid seed culture medium; ), put into shaking table, be 150r/min at rotating speed, under temperature is the condition of 35 DEG C, cultivates 3d, obtain Fermentation Condition of Monascus spp agent.
(3) inoculation fermentation
Less salt Tilapia mossambica block step (1) prepared mixes with the ratio of physiological saline according to 1:1 (w:w), then from the Fermentation Condition of Monascus spp agent that step (2) prepares, isolate the mycelia of monascus, inoculum concentration by 25% joins in fish and water mixture, and add fish and weigh the ground rice of 4.5% as carbon source, then the incubator of 35 DEG C is put into, jolting fermentation 10h;
(4) dry
Entirely be spread out on plastic processing frame by the less salt Tilapia mossambica block fermented in step (3), be placed in hot pump in low temp drying box and carry out cold air drying, wherein, baking temperature is 35 DEG C, and drying time is 10h, in drying course, often stir fish body; Obtain half-dried Tilapia mossambica, the salt content of this half-dried Tilapia mossambica is 5% (w/w), and moisture wet basis is 48% (w/w).
(5) the half-dried Tilapia mossambica that step (4) prepares is put into die pallet, load transparent plastic bag, vacuumize, obtain salt content and be about 5%, water content is about the finished product of 48%.
Effect example
Half-dried Tilapia mossambica prepared by the fermented type half-dried red wine dregs Tilapia mossambica of embodiment 1 being prepared and comparative example 1 and 2 preserves 24 hours at 4 DEG C respectively, open bag, observe and compare the color of three kinds of half-dried fishes under raw state and smell, the results are shown in Table 1, Fig. 2, Fig. 3 and Fig. 4.
The color of three kinds of half-dried Tilapia mossambicas that table 1 is raw and smell
Half-dried Tilapia mossambica prepared by the fermented type half-dried red wine dregs Tilapia mossambica of embodiment 1 being prepared and comparative example 1 and 2 preserves 24 hours at 4 DEG C respectively, takes out and cooks, compare three kinds of half-dried fishes cook after color, smell and flavour, record result, the results are shown in Table 2.
The color of three kinds of half-dried Tilapia mossambicas that table 2 cooks, smell and flavour
With reference to " practical analyzing food nutrition components of mirowave handbook ", China Light Industry Press, the first edition in 2002, chapter 3, the detection method of the one two (four) free amino acid, the free amino acid detected respectively in fermented type half-dried red wine dregs Tilapia mossambica and the comparative example 1 and 2 half-dried Tilapia mossambica of preparing prepared by embodiment 1 forms and content, the results are shown in Table 3.
The free amino acid composition of table 3 three kinds of half-dried Tilapia mossambicas and content
The half-dried Tilapia mossambica that fermented type prepared by the present invention half-dried red wine dregs Tilapia mossambica and comparative example 1 and 2 prepare is compared, the color and luster of product, and smell and flavour all have clear improvement, and has outstanding fermented red beancurd fragrance, and fragrant with certain sauce.Compared with comparative example 2, the present invention does not need jolting, and whole sweat oxygen supply is sufficient, and fermentation is quick and fully, water-solubility protein can not run off because of liquid fermentation, and free aminoacid content is far away higher than comparative example 1 and 2.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (10)
1. a processing method for the half-dried red wine dregs fish of fermented type, is characterized in that comprising the steps:
(1) preparation of less salt fish block
Raw meat being removed through the salt solution cleaning of pretreated fish, then by going the fish after raw meat to pickle with salt, obtaining less salt fish;
(2) preparation of Fermentation Condition of Monascus spp agent
Monascus strain is activated, and prepares Monascus spore suspension; Cultivate in the Monascus spore suspension prepared access liquid seed culture medium, obtain monascus seed liquor; Then cultivate 1 ~ 3 day for 25 ~ 35 DEG C after rice, water, monascus seed liquor being mixed according to the ratio of mass ratio 70: 15: 15, obtain Fermentation Condition of Monascus spp agent;
(3) inoculation fermentation
The Fermentation Condition of Monascus spp agent prepare step (2) and water mix homogenate according to the ratio of mass ratio (1: 1) ~ (5: 1), obtain Fermentation Condition of Monascus spp agent homogenate; The surface uniform coating Fermentation Condition of Monascus spp agent homogenate of the less salt fish then prepared in step (1), in 25 ~ 35 DEG C of bottom fermentation 8 ~ 16h;
(4) dry
By dry for the less salt dried fish fermented in step (3), obtain the half-dried red wine dregs fish of fermented type.
2. the processing method of the half-dried red wine dregs fish of fermented type according to claim 1, is characterized in that:
The mass fraction of the salt solution described in step (1) is 1% ~ 4%.
3. the processing method of the half-dried red wine dregs fish of fermented type according to claim 1, is characterized in that:
The consumption of the salt described in step (1) is 3% ~ 8% of fish quality;
The temperature of pickling described in step (1) is 4 ~ 10 DEG C, and the time of pickling is 1 ~ 5h.
4. the processing method of the half-dried red wine dregs fish of fermented type according to claim 1, is characterized in that:
Monascus strain described in step (2) is GIM3.445 or GIM3.439.
5. the processing method of the half-dried red wine dregs fish of fermented type according to claim 1, is characterized in that:
The concrete steps of the monascus strain activation described in step (2) are: be inoculated into by monascus strain on PDA slant medium, cultivate 3 ~ 5 days, until the Monascus spore maturation on PDA slant medium becomes bronzing for 30 ~ 35 DEG C.
6. the processing method of the half-dried red wine dregs fish of fermented type according to claim 1, is characterized in that:
The formula of the liquid seed culture medium described in step (2) is: ground rice 6.5%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, soybean protein 1.2%, water supply 100%.
7. the processing method of the half-dried red wine dregs fish of fermented type according to claim 1, is characterized in that:
The mass ratio of the less salt fish described in step (3) and Fermentation Condition of Monascus spp agent homogenate is (5:1) ~ (9: 1).
8. the half-dried red wine dregs fish of fermented type, be is characterized in that: prepared by the processing method described in any one of claim 1 ~ 7.
9. the half-dried red wine dregs fish of fermented type according to claim 8, is characterized in that:
The salt content of the half-dried red wine dregs fish of described fermented type is 2% ~ 6%, and moisture wet basis is 30% ~ 55%.
10. the application of the half-dried red wine dregs fish of the fermented type described in claim 8 or 9 in food processing technology field.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510541249.8A CN105054124B (en) | 2015-08-27 | 2015-08-27 | A kind of half-dried red wine dregs fish of fermented type and its processing method and application |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510541249.8A CN105054124B (en) | 2015-08-27 | 2015-08-27 | A kind of half-dried red wine dregs fish of fermented type and its processing method and application |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105054124A true CN105054124A (en) | 2015-11-18 |
CN105054124B CN105054124B (en) | 2019-07-19 |
Family
ID=54483846
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510541249.8A Active CN105054124B (en) | 2015-08-27 | 2015-08-27 | A kind of half-dried red wine dregs fish of fermented type and its processing method and application |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105054124B (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106690124A (en) * | 2016-12-15 | 2017-05-24 | 湖北工业大学 | Method for producing vinasse flavored striped tuna containing rich beta-carotene by utilizing vinasse |
CN109480221A (en) * | 2018-12-22 | 2019-03-19 | 大连民族大学 | It is a kind of by Mucor and the gadus of Fermentation Condition of Monascus spp and preparation method thereof |
JP2020108344A (en) * | 2018-12-28 | 2020-07-16 | 小林製薬株式会社 | Ang-khak-containing fish meat quality improving composition |
CN112244240A (en) * | 2020-05-20 | 2021-01-22 | 三都港海洋食品有限公司 | Vinasse large yellow croaker and fermentation method thereof |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101491350A (en) * | 2009-01-20 | 2009-07-29 | 湘潭大学 | Hongqu fish fermented by mixed bacterium based on lactobacillus and preparation method thereof |
CN101744316A (en) * | 2009-12-18 | 2010-06-23 | 暨南大学 | Fermented low-salt semi-dried tilapia mossambica, processing method thereof and application thereof |
-
2015
- 2015-08-27 CN CN201510541249.8A patent/CN105054124B/en active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101491350A (en) * | 2009-01-20 | 2009-07-29 | 湘潭大学 | Hongqu fish fermented by mixed bacterium based on lactobacillus and preparation method thereof |
CN101744316A (en) * | 2009-12-18 | 2010-06-23 | 暨南大学 | Fermented low-salt semi-dried tilapia mossambica, processing method thereof and application thereof |
Non-Patent Citations (4)
Title |
---|
张宸: "红曲霉发酵半干罗非鱼技术研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
张宸等: "SPME-GC-MS测定红曲霉发酵半干罗非鱼的挥发性成分", 《食品工业科技》 * |
湖南省商业局副食品公司等: "《湖南菜谱》", 31 December 1978, 湖南人民出版社 * |
王乃富等: "红曲霉发酵对鳙鱼肉糜品质和生物活性影响研究", 《广西农学报》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106690124A (en) * | 2016-12-15 | 2017-05-24 | 湖北工业大学 | Method for producing vinasse flavored striped tuna containing rich beta-carotene by utilizing vinasse |
CN109480221A (en) * | 2018-12-22 | 2019-03-19 | 大连民族大学 | It is a kind of by Mucor and the gadus of Fermentation Condition of Monascus spp and preparation method thereof |
JP2020108344A (en) * | 2018-12-28 | 2020-07-16 | 小林製薬株式会社 | Ang-khak-containing fish meat quality improving composition |
CN112244240A (en) * | 2020-05-20 | 2021-01-22 | 三都港海洋食品有限公司 | Vinasse large yellow croaker and fermentation method thereof |
Also Published As
Publication number | Publication date |
---|---|
CN105054124B (en) | 2019-07-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103710253B (en) | A kind of method utilizing yellow seriflux, fermenting bean dregs to produce vinegar | |
CN105077372A (en) | Preparation method of instant leisure flavored fermented fish product | |
CN101248884B (en) | Technological process for preparing fermentation dehydrated beef using composite leaven | |
CN101744316B (en) | Fermented low-salt semi-dried tilapia mossambica, processing method thereof and application thereof | |
CN107518379A (en) | Edible mushroom pickled vegetable product of fermentation and preparation method thereof is delivered directly using lactic acid bacteria | |
CN102352323B (en) | Ester producing yeast as well as method and application of yeast for producing Xiaoqu fen-flavor seasoning wine | |
CN104544312B (en) | A kind of half-fermented less salt flesh of fish leisure food and preparation method thereof | |
CN109673960B (en) | Composite bacterium fermented dried venison and preparation method thereof | |
CN1826963A (en) | Fermented capsicum product and preparation method thereof | |
CN102187997A (en) | Pickled oyster mushrooms and preparation method thereof | |
CN105054124B (en) | A kind of half-dried red wine dregs fish of fermented type and its processing method and application | |
CN102823832B (en) | Method for producing pickled vegetable by inoculated fermentation of Tianjin preserved vegetable soup | |
CN105942299A (en) | Method for preparing functional minced larimichthys crocea products by using mixed microbe fermentation | |
CN104544160A (en) | Production method for fermented beef granules | |
CN105876673A (en) | Method for preparing functional minced small yellow croaker product by utilizing mixed-bacterium fermentation | |
CN105767984A (en) | Fermented chili-pepper product and preparation method thereof | |
CN104489713A (en) | Method for making fermented dried venison | |
CN103584005A (en) | Salicornia paper type vegetable and preparation method | |
CN102987429B (en) | Method for making flavor-conditioning smoked chicken through combination processing of lactobacillus plantarum and pediococcus pentosaceus and radio frequency | |
CN106036561A (en) | Bioprocessing method for conditioning freshwater fish | |
CN105533577A (en) | Method for preparing vegetable-fermented product | |
CN101579011A (en) | Preparation method and application of fermenting agent for dried fish with compound flavors | |
CN106722349A (en) | Using the method for fruits and vegetables zymotic fluid curing salted meat | |
CN103039920A (en) | Biologically fermented black allium chinense and preparation method thereof | |
CN101971998A (en) | Processing method of egg with pickled peppers |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |