CN105348408B - A kind of method of polysaccharide component leaching rate in raising Herba Dendrobii - Google Patents
A kind of method of polysaccharide component leaching rate in raising Herba Dendrobii Download PDFInfo
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- CN105348408B CN105348408B CN201510931205.6A CN201510931205A CN105348408B CN 105348408 B CN105348408 B CN 105348408B CN 201510931205 A CN201510931205 A CN 201510931205A CN 105348408 B CN105348408 B CN 105348408B
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Abstract
The invention discloses a kind of method for improving polysaccharide component leaching rate in Herba Dendrobii, specifically the fresh Herba Dendrobii bar of raw material is cleaned up, dried, cut；It crushed before 100 ~ 300 mesh sieves obtain Herba Dendrobii extraction and expect after expanded broken wall, the water that 4 ~ 8 times of weight is added in expecting before extracting extracts 3 ~ 4 times in 80 ~ 100 DEG C, and 1 ~ 3h, merges extract solution every time, filters, is concentrated to give the medicinal extract that relative density is 1.1 ~ 1.3；By the medicinal extract c dryings of extraction, pulverize and sieve to obtain Objective extraction thing.Small investment of production equipment of the present invention, technological operation is simple, and condition easily reaches, and preparation process energy consumption is low, and cost is low, easy to process, and reagent used is nontoxic, safety and environmental protection；Polysaccharide component in the Herba Dendrobii product of preparation is easy to leach, and improves the leaching rate of polysaccharide component in Herba Dendrobii.
Description
Technical field
The invention belongs to drug preparation technique field, and in particular to a kind of to improve polysaccharide component leaching rate in Herba Dendrobii
Method.
Background technology
Herba Dendrobii (Dendrobium denneanumKerr.) it is one of orchid family Dendrobium Sw line leaf stem of noble dendrobium change
Kind, it is sweet, it is slightly cold, returns stomach, kidney channel, there is the effect of reinforcing stomach reg fluid, nourishing Yin and clearing heat.For impairment of yin body fluid deficiency, dry polydipsia, after being ill
Abnormal heat etc. is demonstrate,proved.Herba Dendrobii chemical composition mainly has polysaccharide, bibenzyl, sequiterpene, cumarin, steroidal etc., wherein based on polysaccharide
Want composition.Polysaccharide has the effect such as enhancing immunity of organisms, hypoglycemic, antitumor, anti-aging, anti-oxidant.
There are the extractive technique that pertinent literature is related to polysaccharide compound in Herba Dendrobii, Luo Aoxue, Li Manfei, Zhang Xi at present
Jade et al. is utilized respectively distilled water reflux extraction, and (Luo Aoxue, Song Guanbin, sieve withstand the frost, Fan Yijun, pure damp orthogonal designs
It is preferred that Herba Dendrobii polysaccharide extracting process [J] traditional Chinese medicine academic periodical 2005,23 (11):1991- 1992;Li Manfei, Xu Guo
Hook, flat Tian Yizheng, assay [J] Chinese herbal medicines 1990,21 (10) of the just military Chinese medicines dendrobium polysaccharide of red plumage:10;
Determination of polysaccharide [J] Leshan Teachers College journal 2004,19 (5) of western tri- kinds of river production artificial cultivation stems of noble dendrobium of beautiful:
88.) extraction to polysaccharide compound in Herba Dendrobii is attempted.These preparation methods belong to conventional method more, and polysaccharide carries
Take that rate is low, solvent-oil ratio is big, operation sequence is complicated, energy consumption is big, time-consuming.Therefore, exploitation one kind can solve above-mentioned technical problem
Method be very important.
The content of the invention
It is an object of the invention to provide a kind of method for improving polysaccharide component leaching rate in Herba Dendrobii.
The object of the present invention is achieved like this, including pre-treatment, extraction, post-processing step, specifically includes：
A, pre-treatment：
1）The fresh Herba Dendrobii bar of raw material is cleaned up, dried, is placed in 90 ~ 100 DEG C of dry 3.5 ~ 4.0h to moisture content
For 15 ~ 25%, 3.5 ~ 4.0cm billet a is cut into；
2）Billet a crushed before 100 ~ 300 mesh sieves obtain Herba Dendrobii extraction after expanded broken wall and expect that b is standby；
B, extract：Material b weight before Herba Dendrobii extracts is added before the Herba Dendrobii extraction that step A is handled to obtain in material b
Water than 4 ~ 8 times extracts 3 ~ 4 times in 80 ~ 100 DEG C, and 1 ~ 3h, merges extract solution every time, filters, is concentrated to give relative density as 1.1
~ 1.3 medicinal extract c；
C, post-process：By the medicinal extract c of extraction through drying, pulverize and sieve to obtain Objective extraction thing.
The present invention has advantages below：
1st, obtained Herba Dendrobii product is produced and processed using microwave bulking broken wall method of the present invention, it is direct-edible or be used as
Dispensing stews edible or brewed for drinking.The product remains flavor, color and luster, mouthfeel and the nutrition of Herba Dendrobii.
2nd, the Herba Dendrobii product prepared using the inventive method, product quality is stable, edible safety.Both tradition is remained
The advantages of medicine materical crude slice, it is easy to people to eat again, polysaccharide component water leaching rate improves, so as to substantially increase the biology profit of Herba Dendrobii
Expenditure, enhance the healthcare function of Herba Dendrobii.
3rd, use energy- and time-economizing, and microwave has sterilization, is protected this have the advantage that process time is short
The safe and healthy of product is demonstrate,proved；In microwave bulking broken wall food is in cellular more, water-soluble property increase, is advantageous to gastro-intestinal digestion enzyme
Infiltration, improve the digestibility of nutrient, be easy to digest and assimilate.
Embodiment
With reference to embodiment, the present invention is further illustrated, but the present invention is not any limitation as in any way,
Based on present invention teach that any conversion or replacement made, belong to protection scope of the present invention.
The method of polysaccharide component leaching rate in raising Herba Dendrobii of the present invention, including pre-treatment, extraction, post processing
Step, specifically include：
A, pre-treatment：
1）The fresh Herba Dendrobii bar of raw material is cleaned up, dried, is placed in 90 ~ 100 DEG C of dry 3.5 ~ 4.0h to moisture content
For 15 ~ 25%, 3.5 ~ 4.0cm billet a is cut into；
2）Billet a crushed before 100 ~ 300 mesh sieves obtain Herba Dendrobii extraction after expanded broken wall and expect that b is standby；
B, extract：Material b weight before Herba Dendrobii extracts is added before the Herba Dendrobii extraction that step A is handled to obtain in material b
Water than 4 ~ 8 times extracts 3 ~ 4 times in 80 ~ 100 DEG C, and 1 ~ 3h, merges extract solution every time, filters, is concentrated to give relative density as 1.1
~ 1.3 medicinal extract c；
C, post-process：By the medicinal extract c of extraction through drying, pulverize and sieve to obtain Objective extraction thing.
Moisture content described in step A is 20 ~ 25%.
Expanded broken wall described in step A is to use microwave bulking broken wall.
The power of described expanded broken wall is 600 ~ 700w, and the time of expanded broken wall is 35 ~ 40s.
Diacolation or refluxing extraction are extracted as described in step B.
The present invention is further described so that case is embodied below：
The assay method for the present invention being prepared polysaccharide component content in Objective extraction thing is as follows：
The information of unit type and manufacturer used in various embodiments of the present invention is as follows：
Micro-wave oven（M-NJL07-3, the long-range Science and Technology Ltd. in Beijing Chinese and Western）；Electric drying oven with forced convection（DHG-9070, on
Shanghai Yiheng Scientific Instruments Co., Ltd of Hai Yiheng Science and Technology Ltd.s）.
（1）The preparation of polysaccharide reference substance solution：Take DEXTROSE ANHYDROUS reference substance appropriate, it is accurately weighed, add water that every 1ml is made
Glucose solution containing 90 μ g, is produced.
（2）The preparation of standard curve：Precision measures the 2ml of reference substance solution 0., 0.4ml, 0. 6ml, 0.8ml, 1.0ml,
L0ml tool plug test tube towel is put respectively, and respectively plus water is mended to 1. 0ml.Precision adds 5% phenol solution 1ml（Face with preparation）, shake up, then
Precision plus sulfuric acid 5ml, shake up, and put boiling water bath towel and heat 20 minutes, take out, put in ice bath and cool down 5 minutes, using corresponding reagent as sky
In vain, using a ultraviolet visible spectrophotometry, absorbance is determined at 488nm wavelength, using absorbance as ordinate, concentration is
Abscissa, draw standard curve.
（3）The preparation of need testing solution:Take this product powder（Cross No. three sieves）About 0. 3g, it is accurately weighed, add water 200ml.
It is heated to reflux 2 hours, lets cool, is transferred in 250ml measuring bottles, with a small amount of moisture time washing container, washing lotion is incorporated in same measuring bottle,
Scale is added water to, is shaken up, is filtered, precision measures subsequent filtrate 2ml, put in 15 ml centrifuge tubes, and precision adds absolute ethyl alcohol l0ml,
Shake up, refrigerate l hours, take out, centrifugation（Rotating speed is 4000 turns per minute）20 minutes, abandoning supernatant, precipitation plus 80% ethanol were washed
Wash 2 times, each 8ml, centrifuge, abandoning supernatant, precipitation plus hot water dissolving, be transferred in 25ml volumetric flasks, let cool, add water to quarter
Degree, shakes up, produces.
（4）Assay method：Precision measures need testing solution 1ml, puts in l0ml tool plug test tubes, under sighting target directrix curve preparation
Method, from " precision add 5% phenol solution 1ml ", determine absorbance in accordance with the law, need testing solution read from standard curve
The amount of middle DEXTROSE ANHYDROUS, calculate, produce.
Comparative example：
A, the fresh fresh bar of Herba Dendrobii is cleaned up, dried；
B, the A fresh bars of Herba Dendrobii walked are placed in drying box and dried；
C, the B Herba Dendrobii billets walked are broken with the expanded broken wall of screw food extruder or other conventional wall-breaking methods
Wall produces；
D, the broken wall part cooling for walking C, through crushing（Cross 200 mesh sieves）Pack and produce.
E, the Herba Dendrobii powder obtained in D step process（10 grams）It is middle add 60 milliliters water in 93 DEG C extract 4 times, often
Secondary 2h, merge extract solution, filter, be concentrated to give the medicinal extract that relative density is 1.2；
F, after the medicinal extract that E step process obtains is dried 8 hours at 60 DEG C, pulverize and sieve（Cross 200 mesh sieves）Obtain mesh
Mark extract.
After measured, the stripping quantity of polysaccharide accounts for the 16.3% of sample total amount before the expanded broken wall of Herba Dendrobii, above-mentioned various broken wall sides
The stripping quantity of Herba Dendrobii polysaccharide after method broken wall accounts for the 19.4 ~ 21.2% of sample total amount.
Embodiment 1
A, the fresh fresh bar of Herba Dendrobii is cleaned up, dried；
B, the A fresh bars of Herba Dendrobii walked are placed in drying box, are dried 3.7 hours at 95 DEG C, make its moisture be
20%, it is cut into 3.7 centimetres of billet；
C, the B Herba Dendrobii billets walked are produced with microwave bulking broken wall；The process conditions of the microwave bulking broken wall are
Power 650w, expanded broken time are 37 seconds, obtain expanded broken wall product；
D, by after the expanded broken wall part cooling 15 minutes of C steps, through crushing（Cross 200 mesh sieves）Pack and produce.
E, the Herba Dendrobii powder obtained in D step process（10 grams）It is middle add 60 milliliters water in 93 DEG C extract 4 times, often
Secondary 2h, merge extract solution, filter, be concentrated to give the medicinal extract that relative density is 1.2；
F, after the medicinal extract that E step process obtains is dried 8 hours at 60 DEG C, pulverize and sieve（Cross 200 mesh sieves）Obtain mesh
Mark extract.
After measured, the stripping quantity of polysaccharide accounts for the 24.8% of sample total amount after the expanded broken wall of Herba Dendrobii, stripping quantity opposing compression
The Herba Dendrobii polysaccharide stripping quantity of expanded broken wall method or other conventional broken wall method broken walls adds 17.5 ~ 27.8%.
Embodiment 2
A, the fresh fresh bar of Herba Dendrobii is cleaned up, dried；
B, the A fresh bars of Herba Dendrobii walked are placed in drying box, are dried 4.0 hours at 90 DEG C, make its moisture be
25%, it is cut into 3.5 centimetres of billet；
C, the B Herba Dendrobii billets walked are produced with microwave bulking broken wall；The process conditions of the microwave bulking broken wall are
Power 600w, expanded broken time are 40 seconds, obtain expanded broken wall product；
D, by after the expanded broken wall part cooling 15 minutes of C steps, through crushing（Cross 300 mesh sieves）Pack and produce.
E, the Herba Dendrobii powder obtained in D step process（10 grams）The middle water for adding 80 milliliters extracts 3 times in 100 DEG C,
Each 3h, merge extract solution, filter, be concentrated to give the medicinal extract that relative density is 1.3；
F, after the medicinal extract that E step process obtains is dried 6 hours at 60 DEG C, pulverize and sieve（Cross 300 mesh sieves）Obtain mesh
Mark extract.
After measured, the stripping quantity of polysaccharide accounts for the 23.9% of sample total amount after the expanded broken wall of Herba Dendrobii, stripping quantity opposing compression
The Herba Dendrobii polysaccharide stripping quantity of expanded broken wall method or other conventional broken wall method broken walls adds 12.7 ~ 23.2%.
Embodiment 3
A, the fresh fresh bar of Herba Dendrobii is cleaned up, dried；
B, the A fresh bars of Herba Dendrobii walked are placed in drying box, are dried 3.5 hours at 100 DEG C, make its moisture
For 15%, 4.0 centimetres of billet is cut into；
C, the B Herba Dendrobii billets walked are produced with microwave bulking broken wall；The process conditions of the microwave bulking broken wall are
Power 700w, expanded broken time are 35 seconds, obtain expanded broken wall product；
D, by after the expanded broken wall part cooling 10 minutes of C steps, through crushing（Cross 100 mesh sieves）Pack and produce.
E, the Herba Dendrobii powder obtained in D step process（10 grams）It is middle add 40 milliliters water in 80 DEG C extract 3 times, often
Secondary 1h, merge extract solution, filter, be concentrated to give the medicinal extract that relative density is 1.1；
F, after the medicinal extract that E step process obtains is dried 10 hours at 60 DEG C, pulverize and sieve（Cross 100 mesh sieves）Obtain
Objective extraction thing.
After measured, the stripping quantity of polysaccharide accounts for the 24.2% of sample total amount after the expanded broken wall of Herba Dendrobii, stripping quantity opposing compression
The Herba Dendrobii polysaccharide stripping quantity of expanded broken wall method or other conventional broken wall method broken walls adds 14.2 ~ 24.7%.
General principle, principal character and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, the premise of spirit and scope of the invention is not being departed from, various changes and modifications of the present invention are possible, these changes
It all fall within the protetion scope of the claimed invention with improvement.The claimed scope of the invention is by appended claims and its waits
Effect thing defines.
Claims (3)
- A kind of 1. method for improving polysaccharide component leaching rate in Herba Dendrobii, it is characterised in that including pre-treatment, extraction, post processing Step, specifically include：A, pre-treatment：1）The fresh Herba Dendrobii bar of raw material is cleaned up, dried, be placed in 90 ~ 100 DEG C of dry 3.5 ~ 4.0h to moisture content be 15 ~ 25%, it is cut into 3.5 ~ 4.0cm billet a；2）Billet a be crushed into 100 ~ 300 mesh sieves after expanded 35 ~ 40s of broken wall under the conditions of 600 ~ 700W of power and obtain folded sheath stone Material b is standby before dry measure used in former times extraction；B, extract：Material b weight is than 4 ~ 8 before adding Herba Dendrobii extraction in material b before the Herba Dendrobii extraction that step A is handled to obtain Water again extracts 3 ~ 4 times in 80 ~ 100 DEG C, and 1 ~ 3h, merges extract solution every time, filters, is concentrated to give relative density as 1.1 ~ 1.3 Medicinal extract c；C, post-process：By the medicinal extract c of extraction through drying, pulverize and sieve to obtain Objective extraction thing.
- 2. the method for polysaccharide component leaching rate in Herba Dendrobii is improved according to claim 1, it is characterised in that institute in step A The moisture content stated is 20 ~ 25%.
- 3. the method for polysaccharide component leaching rate in Herba Dendrobii is improved according to claim 1, it is characterised in that institute in step B That states is extracted as diacolation or refluxing extraction.
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