CN105326978A - Method for extracting longan seed flavone - Google Patents
Method for extracting longan seed flavone Download PDFInfo
- Publication number
- CN105326978A CN105326978A CN201510776155.9A CN201510776155A CN105326978A CN 105326978 A CN105326978 A CN 105326978A CN 201510776155 A CN201510776155 A CN 201510776155A CN 105326978 A CN105326978 A CN 105326978A
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- CN
- China
- Prior art keywords
- flavone
- arillus longan
- seed
- longan
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/77—Sapindaceae (Soapberry family), e.g. lychee or soapberry
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method for extracting longan seed flavone, and belongs to the technical field of agricultural production. The method comprises the following steps that longan seeds are dried at the temperature of 60 DEG C-70 DEG C, mashed and screened with a 300-500-mesh screen, 3000-5000 U/g pectinase and 4000-6000 U/g cellulase are added, enzymolysis is performed for 1-4 h at the temperature of 30 DEG C-40 DEG C, reflux extraction is performed with ethyl alcohol with the concentration ratio of 1:3-1:5 for 5-12 h at the temperature of 50 DEG C-80 DEG C, and vacuum drying is performed on the collected flavone for 5-10 h at the temperature of minus 20 DEG C-30 DEG C. According to the method, operation is easy, the longan flavone can be extracted to the maximum limit, comprehensive utilization of waste materials is achieved, the utilization value of the longan seeds is improved, and the prepared flavone is high in activity.
Description
Technical field
The present invention relates to a kind of method extracting seed of Arillus Longan flavone, belong to field of agricultural production technologies.
Background technology
Arillus Longan (Classification system: DimocarpuslonganLour.) is also known as Arillus Longan, and evergreen megaphanerophyte, tree height is large.Pluck when 7 ~ August, fruit maturation was yellowish-brown.Fruit is eaten raw or is processed into dry products, and meat, core, skin and root all can be done medicinal.Originate in SOUTHERN CHINA and the west and south, have multiple countries and regions to cultivate Arillus Longan in the world, as: China, Thailand, Indonesia, the Queensland state of Australia, the Hawaii of the U.S. and Florida State etc.
Arillus Longan treatment asthenia is frail, insomnia, forgetful, palpitation with fear, palpitation with a distress feeling, dizzy Be very effective of having a guilty conscience.In addition Arillus Longan also has the effect of anti-senility, because it can suppress the activity of a kind of enzyme making people's aging in human body, adds contained rich in protein vitamin and mineral, and food can " make people make light of one's life by commiting suicide not old " for a long time; Arillus Longan can also benefiting qi and nourishing blood, and having good curative effect to neurasthenia, the vexed sweating of climacteric women, the hypophrenia, is the good merchantable brand of nourishing the brain and improving intelligence; And postpartum women body void is weak, or malnutrition causes anemia, and edible Arillus Longan is good selection.Arillus Longan is calmed the nerves, but tired people does not eat; Otherwise can be drowsiness.
Flavone compound makes a general reference two phenyl ring (A-and B-ring) with phenolic hydroxyl group by the interconnected a series of compounds of central thricarbon atom, and its basic parent nucleus is 2-phenyl chromone.The functional groups such as phenolic hydroxyl group, methoxyl group, methyl, isopentene group are often connected with in flavone compound structure.In addition, it also normal and sugar be combined into glycosides.
Chinese patent, application number: the 201410129369.2 smart extracting method disclosing a kind of sweet potato starch, comprise the following steps: (1) is not cleaned Radix Ipomoeae, are directly pulverized by Radix Ipomoeae, obtain sweet potato paste; (2) by sweet potato paste dilute with water, the weight ratio of sweet potato paste and water is 1: 3 ~ 5; Then filter, obtain filtrate; (3) in filtrate, add the mixed liquor of the grounds travel of Gypsum Fibrosum, Folium Ipomoea extract and soybean oil, the addition of mixed liquor is 0.1% of filtrate weight, then stirs; During preparation mixed liquor, the weight proportion of the grounds travel of Gypsum Fibrosum, Folium Ipomoea extract and soybean oil is 10: 1: 1, first the grounds travel of Gypsum Fibrosum is put into water and be dissolved into saturated parget water, then add Folium Ipomoea extract and soybean oil, stir and obtain mixed liquor.(4) after stirring, staticly settle, in filtrate, earth, sweet potato starch and aquiform stool are separated into upper, middle and lower-ranking, and (5) drain the water on upper strata, then take out sweet potato starch; (6) sweet potato starch dried or dry, dress bag.But the method extraction step is complicated, being only extract starch, is not extract flavone.Therefore develop a kind of method tool extracting seed of Arillus Longan flavone to be of great significance.
Summary of the invention
The object of this invention is to provide a kind of method extracting seed of Arillus Longan flavone, the present invention is simple to operate, can comprehensive utilization of waste materials, increases the value of seed of Arillus Longan.
In order to realize the object of foregoing invention, the technical solution adopted in the present invention is:
Extract a method for seed of Arillus Longan flavone, comprise the following steps:
Seed of Arillus Longan is dry at 60-70 DEG C, blend, cross 300-500 mesh sieve, add pectase 3000-5000U/g, cellulase 4000-6000U/g, enzymolysis 1-4h at 30-40 DEG C, with the ethanol of 1:3-1:5, reflux, extract, 5-12h at 50-80 DEG C, by the flavone collected at-20 DEG C--vacuum drying 5-10h at 30 DEG C.
Raw material Arillus Longan needs the Arillus Longan adopting Guangdong Huizhou to produce.
Extracting ethanol can adopt acetone to substitute.
Flavone after vacuum drying can be prepared into powdery and preserve.
the present invention relative to the beneficial effect of prior art is:
The present invention is simple to operate, can comprehensive utilization of waste materials, increases the value of seed of Arillus Longan; The flavonoid active of preparation is high, can extract Arillus Longan flavone to greatest extent.
Detailed description of the invention
Be described in further details the present invention below by embodiment, these embodiments are only used for the present invention is described, do not limit the scope of the invention.
Embodiment 1
Extract a method for seed of Arillus Longan flavone, comprise the following steps:
Seed of Arillus Longan is dry at 60 DEG C, and blend, cross 300 mesh sieve, add pectase 3000U/g, cellulase 4000U/g, enzymolysis 1h at 30 DEG C, with the ethanol of 1:3, reflux, extract, 5h at 50 DEG C, by the flavone vacuum drying 5h at-20 DEG C collected.
Raw material Arillus Longan needs the Arillus Longan adopting Guangdong Huizhou to produce.
Embodiment 2
Extract a method for seed of Arillus Longan flavone, comprise the following steps:
Seed of Arillus Longan is dry at 62 DEG C, and blend, cross 400 mesh sieve, add pectase 4000U/g, cellulase 5000U/g, enzymolysis 2h at 32 DEG C, with the ethanol of 1:4, reflux, extract, 7h at 60 DEG C, by the flavone vacuum drying 6h at-24 DEG C collected.
Raw material Arillus Longan needs the Arillus Longan adopting Guangdong Huizhou to produce.
Embodiment 3
Extract a method for seed of Arillus Longan flavone, comprise the following steps:
Seed of Arillus Longan is dry at 66 DEG C, and blend, cross 500 mesh sieve, add pectase 5000U/g, cellulase 6000U/g, enzymolysis 4h at 36 DEG C, with the ethanol of 1:5, reflux, extract, 9h at 70 DEG C, by the flavone vacuum drying 7h at-27 DEG C collected.
Raw material Arillus Longan needs the Arillus Longan adopting Guangdong Huizhou to produce.
Embodiment 4
Extract a method for seed of Arillus Longan flavone, comprise the following steps:
Seed of Arillus Longan is dry at 68 DEG C, and blend, cross 500 mesh sieve, add pectase 5000U/g, cellulase 4000U/g, enzymolysis 4h at 38 DEG C, with the flavone of 1:4, reflux, extract, 10h at 70 DEG C, by the flavone vacuum drying 10h at-20 DEG C collected.
Raw material Arillus Longan needs the Arillus Longan adopting Guangdong Huizhou to produce.
Embodiment 5
Extract a method for seed of Arillus Longan flavone, comprise the following steps:
Seed of Arillus Longan is dry at 70 DEG C, and blend, cross 500 mesh sieve, add pectase 5000U/g, cellulase 6000U/g, enzymolysis 4h at 40 DEG C, with the flavone of 1:5, reflux, extract, 12h at 80 DEG C, by the flavone vacuum drying 10h at-30 DEG C collected.
Raw material Arillus Longan needs the Arillus Longan adopting Guangdong Huizhou to produce.
Claims (3)
1. extract a method for seed of Arillus Longan flavone, it is characterized in that: comprise the following steps:
Seed of Arillus Longan is dry at 60-70 DEG C, blend, cross 300-500 mesh sieve, add pectase 3000-5000U/g, cellulase 4000-6000U/g, enzymolysis 1-4h at 30-40 DEG C, then uses the ethanol of 1:3-1:5, reflux, extract, 5-12h at 50-80 DEG C, the flavone collected is at-20 DEG C--vacuum drying 5-10h at 30 DEG C.
2. a kind of method extracting seed of Arillus Longan flavone according to claim 1, is characterized in that:
Extracting ethanol can adopt acetone to substitute.
3. a kind of method extracting seed of Arillus Longan flavone according to claim 1, is characterized in that:
Flavone after vacuum drying can be prepared into powdery and preserve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510776155.9A CN105326978A (en) | 2015-11-13 | 2015-11-13 | Method for extracting longan seed flavone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510776155.9A CN105326978A (en) | 2015-11-13 | 2015-11-13 | Method for extracting longan seed flavone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105326978A true CN105326978A (en) | 2016-02-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510776155.9A Pending CN105326978A (en) | 2015-11-13 | 2015-11-13 | Method for extracting longan seed flavone |
Country Status (1)
| Country | Link |
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| CN (1) | CN105326978A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101433620A (en) * | 2008-12-10 | 2009-05-20 | 广东省农业科学院农业生物技术研究所 | Longan water-soluble active substance as well as extraction method and use thereof |
| CN104288313A (en) * | 2014-10-31 | 2015-01-21 | 于芳 | Method for extracting flavone from longan seeds |
-
2015
- 2015-11-13 CN CN201510776155.9A patent/CN105326978A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101433620A (en) * | 2008-12-10 | 2009-05-20 | 广东省农业科学院农业生物技术研究所 | Longan water-soluble active substance as well as extraction method and use thereof |
| CN104288313A (en) * | 2014-10-31 | 2015-01-21 | 于芳 | Method for extracting flavone from longan seeds |
Non-Patent Citations (1)
| Title |
|---|
| 彭晓青等: "复合酶法提取龙眼核总黄酮的工艺研究", 《中国医院用药评价与分析》 * |
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Application publication date: 20160217 |