CN105326869A - Antitumor composition containing crocodile extract and preparation method thereof - Google Patents
Antitumor composition containing crocodile extract and preparation method thereof Download PDFInfo
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- CN105326869A CN105326869A CN201510717211.1A CN201510717211A CN105326869A CN 105326869 A CN105326869 A CN 105326869A CN 201510717211 A CN201510717211 A CN 201510717211A CN 105326869 A CN105326869 A CN 105326869A
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/58—Reptiles
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/04—Sulfur, selenium or tellurium; Compounds thereof
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
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Abstract
The invention discloses an antitumor composition containing a crocodile extract and a preparation method thereof and belongs to the technical field of medicines. The composition and the preparation method thereof enhance immunity and are good in antitumor effect. The antitumor composition is technically characterized by being prepared from, by weight, 20-80 parts of a crocodile blood extract, 20-80 parts of crocodile shell extract, 10-20 parts of lucid and 1-5 parts of selenium.
Description
Technical field
The open a kind of clear anti-tumor compositions of the present invention, specifically, is a kind of anti-tumor compositions containing crocodile extract, belongs to medical art.
Background technology
Tumor refers to that body is under the effect of the various tumorigenesis factor, the neoplasm that local organization hyperplasia is formed, because this neoplasm is many in occupancy block-like protrusions, also claims vegetation.
Crocodile and dinosaur contemporary, be one of animal the most ancient on the earth, have the title of " living fossil ".One of animal of crocodile longest-lived simultaneously or on the earth, the life-span up to more than 200 year old, than Testudinis and Trionyx sinensis Wiegmann also long.In addition, the animal that on crocodile or the earth, the bodily form is maximum, find that maximum crocodile fish length has 12 meters at present in Africa, weight is up to 8.The Shennong's Herbal of Qin-Han times claims crocodile to be Tuo fish, and cure mainly trusted subordinate's lump in the abdomen, Fu Jian gathers, cold and heat, woman's metrorrhagia hematochezia five colors, phase dragging pain in lower abdomen the moon, the dead flesh of scabies.Record crocodile first soup in the Thousand Golden Prescriptions of the Tang Dynasty " king of medicine " Sun Simiao to cure mainly " wind is insane ".
Summary of the invention
The object of the present invention is to provide a kind of enhancing immunity, the composition and method of making the same that antitumous effect is good.
For solving the problems of the technologies described above, last technical scheme provided by the invention is such:
Containing an anti-tumor compositions for crocodile extract, described compositions is made up of the component of following weight parts: crocodile blood extract 20-80 part, crocodile finger extract 20-80 part, Ganoderma 10-20 part, selenium 1-5 part.
Further, the above-mentioned anti-tumor compositions containing crocodile extract, described compositions is made up of the component of following weight parts: crocodile blood extract 30-60 part, crocodile finger extract 30-60 part, Ganoderma 12-18 part, selenium 2-4 part.
In order to prepare said composition, after provided by the invention, a technical scheme is such:
Containing the preparation method of the anti-tumor compositions of crocodile extract, should comprise the steps: successively
1) crocodile blood extract is extracted respectively, crocodile finger extract;
2) each component is taken;
3) by step 2) after the Ganoderma that takes carries out ultraviolet sterilization with crocodile blood extract, crocodile finger extract, selenium after pulverizing and sieving, the abundant mix homogeneously of input blender, obtains premix material;
4) by premix material tabletting on tablet machine, at 60-80 DEG C, dry 3-5 hour, to obtain final product.
Further, the preparation method of the above-mentioned anti-tumor compositions containing crocodile extract, the preparation method of described crocodile blood extract is:
1) preparation of hemoglobin
Add food grade anticoagulant to fresh crocodile blood, fully stir and centrifugalize, by lower floor's hemocyte brine, the hemocyte of collecting precipitation adds distilled water, and with the process of ultrasonic grinding instrument, centrifugal segregation cell membrane and impurity, obtain hemoglobin solutions;
2) enzyme solution of hemoglobin
Get hemoglobin solutions, be adjusted to anti-pH value 6.5-7.5 by NaOH solution, add in mmp reaction process and be adjusted to pH value 7.5 by NaOH solution; Reaction terminates rear enzyme denaturing, cessation reaction, obtains hydrolyzed solution;
3) pretreatment of hydrolyzed solution
By the hydrolyzed solution centrifugalize of enzyme denaturing, supernatant carries out decolouring, debitterize process, repeatedly centrifugally obtains hemoglobin hydrolyzed solution;
4) purification of Hemoglobin Peptide
By hemoglobin hydrolyzed solution dialysis treatment, obtain the outer transparent liquid that molecular weight is less than 8000, by outer transparent liquid vacuum lyophilization, Hemoglobin Peptide sample preparation is become the solution of 5ug/ml concentration, get appropriate amount of sample and carry out purification by ion exchange chromatography, gel filtration chromatography, high performance liquid chromatography successively, finally obtain purer Hemoglobin Peptide sample.
Further, the preparation method of the above-mentioned anti-tumor compositions containing crocodile extract, the preparation method of described crocodile finger extract is: crocodile finger is put into extraction pot, uses soak by water secondary, and each water consumption is the 5-7 times amount of crocodile finger weight, decoct secondary, each 60 ~ 90 minutes, filter, filtrate is condensed into and surveys relative density at 60-65 DEG C is the clear paste of 1.10-1.25, lyophilization, obtains crocodile finger extract.
Compared with prior art, technical scheme provided by the invention has good antitumous effect, wherein, in crocodile blood, crocodile finger containing protein, calcium, ferrum, zinc 1, vitamin A 5, vitamin E, taurine, cholesterol, fat, containing multiple human body essential amino acid, unsaturated fatty acid, vitamin and 33 kinds of trace element, wherein have more than 10 kinds to be the essential trace element favourable to the mankind, and ferrum and Zn content higher; Particularly in crocodile blood, crocodile finger, oxygen carrying capacity exceedes more than 100 times of other animal, has extremely strong suppression and kill ability to tumor cell; Ganoderma energy enhancing human body immunity power, protects human body from antibacterial, virus infraction, anti-tumor processed, the substances such as the polysaccharide in Ganoderma, triterpene, organic germanium penetrate in cancerous cell with telomere covalent bond, make enzyme molecule elongate degeneration, tumoricidal telomerase activation, makes cancer cell-apoptosis; Selenium can promote propagation and the lethal effect of T cell and neutrophilic granulocyte, the synthesis of Promote immunity globulin and antibody tormation, strengthen the antiviral ability of human body, suppress viral activation, and remove oxygen-derived free radicals too much in human body, prevent the snperoxiaized destruction of Cell membrane lipids, its anti-radical action is vitamin E and ascorbic 300 ~ 500 times; Improve overall immunity, there is auxiliary cancer-resisting function.Four coordinative roles, have good inhibition to cancerous cell.
Detailed description of the invention
Below in conjunction with detailed description of the invention; claim of the present invention is described in further detail; but become any limitation of the invention not, the amendment of anyone limited number of time made within the scope of the claims in the present invention, still at claims of the present invention.
Embodiment 1
A kind of anti-tumor compositions containing crocodile extract provided by the invention, described compositions is made up of the component of following weight parts: crocodile blood extract 80kg, crocodile finger extract 20kg, Ganoderma 20kg, selenium 1kg.
Embodiment 2
A kind of anti-tumor compositions containing crocodile extract provided by the invention, described compositions is made up of the component of following weight parts: crocodile blood extract 20kg, crocodile finger extract 80kg, Ganoderma 10kg, selenium 5kg.
Embodiment 3
A kind of anti-tumor compositions containing crocodile extract provided by the invention, described compositions is made up of the component of following weight parts: crocodile blood extract 60kg, crocodile finger extract 30kg, Ganoderma 18kg, selenium 2kg.
Embodiment 4
A kind of anti-tumor compositions containing crocodile extract provided by the invention, described compositions is made up of the component of following weight parts: crocodile blood extract 30kg, crocodile finger extract 60kg, Ganoderma 12kg, selenium 4kg.
Embodiment 5
A kind of anti-tumor compositions containing crocodile extract provided by the invention, described compositions is made up of the component of following weight parts: crocodile blood extract 45kg, crocodile finger extract 45kg, Ganoderma 9kg, selenium 3kg.
In embodiment 1 to 5 any one group containing the preparation method of anti-tumor compositions of crocodile extract, comprise the steps: successively
1) crocodile blood extract is extracted respectively, crocodile finger extract;
2) each component is taken by executing any one group of mass number in example 1 to 5;
3) by step 2) after the Ganoderma that takes carries out ultraviolet sterilization with crocodile blood extract, crocodile finger extract, selenium after pulverizing and sieving, the abundant mix homogeneously of input blender, obtains premix material;
4) by premix material tabletting (every sheet 0.5g) on tablet machine, at 60-80 DEG C, dry 3-5 hour, to obtain final product.
The preparation method of described crocodile blood extract is:
1) preparation of hemoglobin
Add food grade anticoagulant to fresh crocodile blood, fully stir and centrifugalize, by lower floor's hemocyte brine, the hemocyte of collecting precipitation adds distilled water, and with the process of ultrasonic grinding instrument, centrifugal segregation cell membrane and impurity, obtain hemoglobin solutions;
2) enzyme solution of hemoglobin
Get hemoglobin solutions, be adjusted to anti-pH value 6.5-7.5 by NaOH solution, add in neutral protease course of reaction and be adjusted to pH value 7.5 by NaOH solution; Reaction terminates rear enzyme denaturing, cessation reaction, obtains hydrolyzed solution;
3) pretreatment of hydrolyzed solution
By the hydrolyzed solution centrifugalize of enzyme denaturing, supernatant carries out decolouring, debitterize process, repeatedly centrifugally obtains hemoglobin hydrolyzed solution;
4) purification of Hemoglobin Peptide
By hemoglobin hydrolyzed solution dialysis treatment, obtain the outer transparent liquid that molecular weight is less than 8000, by outer transparent liquid vacuum lyophilization, Hemoglobin Peptide sample preparation is become the solution of 5ug/ml concentration, get appropriate amount of sample and carry out purification by ion exchange chromatography, gel filtration chromatography, high performance liquid chromatography successively, finally obtain purer Hemoglobin Peptide sample.
The preparation method of described crocodile finger extract is:
Crocodile finger is put into extraction pot, use soak by water secondary, each water consumption is 5-7 doubly (preferably 6 times) amount of crocodile finger weight, decoct secondary, each 60 ~ 90 minutes (preferably 80 minutes), filter, and filtrate is condensed into and surveys relative density at 60-65 DEG C is the clear paste of 1.10-1.25, lyophilization, obtains crocodile finger extract.
Clinical data
Patient originates: case 100 example is observed in this test altogether, and men and women half and half.
Efficacy determination:
1) effective: tumor tumor body reduces more than 10%, cardinal symptom disappears;
2) effective: tumor tumor body reduces 1 ~ 10%, and clinical symptoms is obviously improved;
3) invalid: tumor tumor body continues to increase, and clinical symptoms is without improvement or increase the weight of.
Test method:
Adopt single blind completely random grouping, tumor observation group treats with compositions provided by the invention, three times on the one, each 1; Matched group adopts chemotherapy and radiotherapy in the treatment.Within 30 days, be a course for the treatment of, observe 3 months.
Two groups of comprehensive therapeutic effects, in table 1:
Table 1
| Group | Number of cases | Effective | Effectively | Invalid | Total effective rate |
| Observation group | 100 | 55 | 40 | 5 | 95％ |
| Matched group | 100 | 27 | 52 | 21 | 79％ |
As can be seen from the table, between two groups, there were significant differences for comprehensive therapeutic effect, and observation group is obviously better than matched group.
Claims (5)
1., containing an anti-tumor compositions for crocodile extract, it is characterized in that, described compositions is made up of the component of following weight parts: crocodile blood extract 20-80 part, crocodile finger extract 20-80 part, Ganoderma 10-20 part, selenium 1-5 part.
2. the anti-tumor compositions containing crocodile extract according to claim 1, it is characterized in that, described compositions is made up of the component of following weight parts: crocodile blood extract 30-60 part, crocodile finger extract 30-60 part, Ganoderma 12-18 part, selenium 2-4 part.
3. the preparation method of the anti-tumor compositions containing crocodile extract according to claim 1, is characterized in that, comprise the steps: successively
1) crocodile blood extract is extracted respectively, crocodile finger extract;
2) each component is taken by mass number according to claim 1;
3) by step 2) after the Ganoderma that takes carries out ultraviolet sterilization with crocodile blood extract, crocodile finger extract, selenium after pulverizing and sieving, the abundant mix homogeneously of input blender, obtains premix material;
4) by premix material tabletting on tablet machine, at 60-80 DEG C, dry 3-5 hour, to obtain final product.
4. the preparation method of the anti-tumor compositions containing crocodile extract according to claim 3, it is characterized in that, the preparation method of described crocodile blood extract is:
1) preparation of hemoglobin
Add food grade anticoagulant to fresh crocodile blood, fully stir and centrifugalize, by lower floor's hemocyte brine, the hemocyte of collecting precipitation adds distilled water, and with the process of ultrasonic grinding instrument, centrifugal segregation cell membrane and impurity, obtain hemoglobin solutions;
2) enzyme solution of hemoglobin
Get hemoglobin solutions, be adjusted to anti-pH value 6.5-7.5 by NaOH solution, add in mmp reaction process and be adjusted to pH value 7.5 by NaOH solution; Reaction terminates rear enzyme denaturing, cessation reaction, obtains hydrolyzed solution;
3) pretreatment of hydrolyzed solution
By the hydrolyzed solution centrifugalize of enzyme denaturing, supernatant carries out decolouring, debitterize process, repeatedly centrifugally obtains hemoglobin hydrolyzed solution;
4) purification of Hemoglobin Peptide
By hemoglobin hydrolyzed solution dialysis treatment, obtain the outer transparent liquid that molecular weight is less than 8000, by outer transparent liquid vacuum lyophilization, Hemoglobin Peptide sample preparation is become the solution of 5ug/ml concentration, get appropriate amount of sample and carry out purification by ion exchange chromatography, gel filtration chromatography, high performance liquid chromatography successively, finally obtain purer Hemoglobin Peptide sample.
5. the preparation method of the anti-tumor compositions containing crocodile extract according to claim 3, it is characterized in that, the preparation method of described crocodile finger extract is: crocodile finger is put into extraction pot, uses soak by water secondary, and each water consumption is the 5-7 times amount of crocodile finger weight, decoct secondary, each 60 ~ 90 minutes, filter, filtrate is condensed into and surveys relative density at 60-65 DEG C is the clear paste of 1.10-1.25, lyophilization, obtains crocodile finger extract.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510717211.1A CN105326869A (en) | 2015-10-28 | 2015-10-28 | Antitumor composition containing crocodile extract and preparation method thereof |
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| CN201510717211.1A CN105326869A (en) | 2015-10-28 | 2015-10-28 | Antitumor composition containing crocodile extract and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108148882A (en) * | 2018-01-05 | 2018-06-12 | 百生康（北京）健康产业科技有限公司 | A kind of extracting method of crocodile first peptide |
| CN109248177A (en) * | 2018-08-23 | 2019-01-22 | 许瑞安 | The preparation of crocodile first active principle and its anti-oxidant, anti-hepatic fibrosis application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1383889A (en) * | 2001-04-27 | 2002-12-11 | 关令华 | Crocodile pill |
| CN103230587A (en) * | 2013-05-10 | 2013-08-07 | 汪华东 | Composition of crocodile hemoglobin peptide and preparation method of composition |
-
2015
- 2015-10-28 CN CN201510717211.1A patent/CN105326869A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1383889A (en) * | 2001-04-27 | 2002-12-11 | 关令华 | Crocodile pill |
| CN103230587A (en) * | 2013-05-10 | 2013-08-07 | 汪华东 | Composition of crocodile hemoglobin peptide and preparation method of composition |
Non-Patent Citations (2)
| Title |
|---|
| 于大元: "《抗癌健身法》", 31 July 2004, 地震出版社 * |
| 汪华东: "《鳄鱼百科》", 30 June 2015, 暨南大学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108148882A (en) * | 2018-01-05 | 2018-06-12 | 百生康（北京）健康产业科技有限公司 | A kind of extracting method of crocodile first peptide |
| CN109248177A (en) * | 2018-08-23 | 2019-01-22 | 许瑞安 | The preparation of crocodile first active principle and its anti-oxidant, anti-hepatic fibrosis application |
| CN109248177B (en) * | 2018-08-23 | 2023-02-28 | 许瑞安 | Preparation of crocodile amour effective component and application of crocodile amour effective component in oxidation resistance and hepatic fibrosis resistance |
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Application publication date: 20160217 |