CN1053220C - Enzyme process for producing L-malic acid - Google Patents
Enzyme process for producing L-malic acid Download PDFInfo
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- CN1053220C CN1053220C CN95111164A CN95111164A CN1053220C CN 1053220 C CN1053220 C CN 1053220C CN 95111164 A CN95111164 A CN 95111164A CN 95111164 A CN95111164 A CN 95111164A CN 1053220 C CN1053220 C CN 1053220C
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- malic acid
- acid
- enzyme
- fumarate
- producing
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- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
The present invention relates to a novel technological process for producing L-malic acid by a novel enzyme method, which belongs to the technical field of enzyme engineering. The technological process has the steps that fumarate is added into a biological reaction crystallizer provided with fumaric acid enzyme; enzyme reaction is carried out at the normal temperature under the atmospheric pressure; crystals of L-malic acid salt are directly obtained through separating and coupling; then, finished products of the L-malic acid are prepared through separation and purification. The technological process of the present invention realizes the complete conversion of the fumarate into the L-malic acid by a one-step method, and has the advantages of high enzyme conversion efficiency, short time, high concentration of unit enzyme conversion generation products, production cost reduction and production capacity improvement.
Description
What the present invention relates to is a kind of new process of novel Production by Enzymes L MALIC ACID, belongs to the enzyme engineering field.L MALIC ACID is at industry, food and the product in wide market is arranged aspect other, as important chemical material, superior as acidic flavoring agent on food than citric acid, its organic acid that can be used as nuisanceless specification substitutes tripoly phosphate sodium STPP and nitrilotriacetic acid sodium commonly used at other industrial circles, eliminates scrub raffinate and enters the disadvantage that the river produces algae.At present, the former immobilized enzyme production technique of L MALIC ACID, transformation efficiency is low, Production Flow Chart is long, and product content is low in the conversion fluid, and water, electricity, manpower consumption are big, back extraction cost is higher, causes that price is too high, L MALIC ACID is sold be difficult to strive unexpectedly with DL-oxysuccinic acid, citric acid.
The objective of the invention is to be to avoid not enough place of the prior art, adopt the novel enzyme reaction and separate the lotus root technology of closing and produce L MALIC ACID, can improve concentration of reactants greatly, realize that single stage method is converted into L MALIC ACID fully with fumarate, obviously improve separation yield, simplify technological process, production energy consumption is few, and quality product is better than chemical synthesis.
Enzyme process for producing L-malic acid takes following scheme to realize: the processing method of novel Production by Enzymes L MALIC ACID is the biological respinse crystallizer of packing into of weighing FURAMIC ACID according to a certain ratio, fumarate is added in the biological respinse crystallizer that FURAMIC ACID is housed according to a certain ratio, under normal temperature, normal pressure, carry out enzyme reaction, separating lotus root closes, reaction, separation, fashionable of lotus root are 20-60 hour, directly obtain the crystallization of L MALIC ACID, separate then, purifying, produce and prepare the L MALIC ACID finished product.
Fumarate and FURAMIC ACID proportioning raw materials (weight ratio) are in the processing method of Production by Enzymes L MALIC ACID: fumarate: FURAMIC ACID=1-6: 0.2-2 (weight ratio).Fumarate can adopt Fumaric acid, calcium salt.
The present invention further describes below in conjunction with example:
1, the 2.5Kg FURAMIC ACID of in the biological respinse crystallizer of 10L, packing into, add Fumaric acid, calcium salt 6.25Kg, carry out enzyme reaction and separate lotus root and close that average conversion is 98.5% after 30 hours, per kilogram (liter) enzyme can transform the L MALIC ACID of producing about 2.5Kg, separate then, purifying is prepared the L MALIC ACID finished product.
2, pack in the biological respinse crystallizer of the 20L FURAMIC ACID of 3kg adds the 12kg Fumaric acid, calcium salt and carries out enzyme reaction and close with separating lotus root, and average conversion reaches 99% after 30 hours, separates then, and purifying is prepared-oxysuccinic acid 9.6kg.
3, the FURAMIC ACID liquid that separates the 250kg that packs in the coupling apparatus at the biological respinse of 2000L, add Fumaric acid, calcium salt 800kg with certain speed, carry out enzyme and instead just close with separating lotus root, average conversion reaches 99% after 36 hours, separate then, purifying, prepare L MALIC ACID 669kg.
Enzyme process for producing L-malic acid has the following advantages than prior art:
This process shows that novel enzymatic conversion method is produced L MALIC ACID and can be reached reaction and divide From carrying out synchronously, realize that one-step method is converted into L MALIC ACID fully with fumarate, and enzyme turns to Change the efficient height, the reaction time is short, and the unit enzymatic conversion generates the concentration height of product, and this can make L-The malic acid production cost reduces by 30%, and production capacity greatly improves. Novel Production by Enzymes L The DL-malic acid that-malic acid can make (1) production cost and chemical method synthesize is suitable, ( 2) the process units investment is low, and energy consumption is few under normal temperature, the normal pressure, and (3) product quality is excellent In chemical synthesis, thereby can significantly strengthen the market competition of L MALIC ACID product.
Claims (3)
1, a kind of novel method of Production by Enzymes L MALIC ACID, it is characterized in that by proportioning weighing FURAMIC ACID, the biological respinse crystallizer of packing into, the fumarate adding is equipped with in the biological respinse crystallizer of FURAMIC ACID, carry out enzyme reaction at normal temperatures and pressures, separate lotus root and close, directly obtain the crystallization of L MALIC ACID salt, separate then, purifying, prepare the L MALIC ACID finished product.
2, enzyme process for producing L-malic acid according to claim 1 is characterized in that fumarate and FURAMIC ACID proportioning raw materials are: fumarate: FURAMIC ACID=1-6: 0.2-2 (weight ratio).
3,, it is characterized in that fumarate adopts Fumaric acid, calcium salt according to claim 1,2 described enzyme process for producing L-malic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95111164A CN1053220C (en) | 1995-08-22 | 1995-08-22 | Enzyme process for producing L-malic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95111164A CN1053220C (en) | 1995-08-22 | 1995-08-22 | Enzyme process for producing L-malic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1124777A CN1124777A (en) | 1996-06-19 |
| CN1053220C true CN1053220C (en) | 2000-06-07 |
Family
ID=5078477
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95111164A Expired - Fee Related CN1053220C (en) | 1995-08-22 | 1995-08-22 | Enzyme process for producing L-malic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1053220C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103910621B (en) * | 2013-12-30 | 2015-09-09 | 西安万隆制药股份有限公司 | A kind of L MALIC ACID compound |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067451A (en) * | 1991-06-04 | 1992-12-30 | 中国科学院微生物研究所 | Production of l-malic acid by fixed cells |
| CN1081206A (en) * | 1992-07-14 | 1994-01-26 | 中国药科大学 | Method with preparing malic acid with enzyme engineering technology |
| CN1093752A (en) * | 1993-04-16 | 1994-10-19 | 欧阳平凯 | C-malic acid production by using immobilized cell |
-
1995
- 1995-08-22 CN CN95111164A patent/CN1053220C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067451A (en) * | 1991-06-04 | 1992-12-30 | 中国科学院微生物研究所 | Production of l-malic acid by fixed cells |
| CN1081206A (en) * | 1992-07-14 | 1994-01-26 | 中国药科大学 | Method with preparing malic acid with enzyme engineering technology |
| CN1093752A (en) * | 1993-04-16 | 1994-10-19 | 欧阳平凯 | C-malic acid production by using immobilized cell |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1124777A (en) | 1996-06-19 |
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