CN105316234B - The method of the method and industrial waste gas denitration of cultivating microalgae - Google Patents
The method of the method and industrial waste gas denitration of cultivating microalgae Download PDFInfo
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Abstract
The present invention relates to a kind of method of cultivating microalgae, this method can either promote the growth of microalgae, and can enhance the ability that microalgae resists miscellaneous bacteria and pest and disease damage, therefore be more suitable for large-scale farming microalgae by the way that EM bacterium is added in the breeding process.The invention further relates to a kind of method of industrial waste gas denitration, this method has higher absorption efficiency to NOx using algae raffinate is supported caused by cultivating microalgae as the absorbing liquid of NOx.
Description
Technical field
The present invention relates to a kind of methods of cultivating microalgae and a kind of method of industrial waste gas denitration.
Background technique
Energy and environment are the major issues that human social is faced.On the one hand, the modern text of the support mankind
Bright fossil energy is non-renewable, and exploitation alternative energy source is extremely urgent；It on the other hand, can not when processing and using fossil energy
The emission problem of exhaust gas and sewage can be generated with avoiding, serious influence is caused on environment, these problems need system
Raise the solution of coordination.
Microalgae is many kinds of and the extremely extensive aquatic rudimentary plant of distribution.They pass through efficient photosynthesis, will
Luminous energy is converted into the chemical energy of the carbohydrate such as fat or starch, is known as " the activation factory of sunlight driving ".Utilize microalgae
Production bioenergy and chemicals are expected to reach the dual purpose of " substitution fossil energy, purification exhaust gas and sewage " simultaneously.
Difficulty known to one of cultivating microalgae is that there are a large amount of harmful bacterias in the water of cultivating microalgae, in open type
Breeding process in also inevitably pollute harmful bacteria, these harmful bacterias are unfavorable for the growth of microalgae, meeting when serious
Cultivation is caused to fail.Although stringent sterilization and it is closed under the conditions of cultivation can to avoid this harm, for
For large-scale farming microalgae, the cost of this method is prohibitively expensive.For these reasons, there is an urgent need to develop one kind to be more suitable for
The method of large-scale farming microalgae makes microalgae in its natural state, can fast-growth and harmful bacteria pollution shadow
It rings.
In nature, there is complicated ecological relationship between microalgae and bacterium, it may mutually promote, it is also possible to phase mutual inhibition
System.Existing certain references disclose the symbiosis between microalgae and bacterium, and on the one hand these documents have only related to one or more
The symbiosis of specific bacterium and microalgae；On the other hand these documents are the generations that other side is mutually utilized by microalgae and bacterium
Thank to product, to achieve the purpose that growth of mutually promoting.So far, it yet there are no and " in microalga cultivation process, use profitable strain
The relevant report of prevention and treatment harmful bacteria pollution ".
Nitrogen is consumption most one of fast, the nutrient most easily lacked during micro algae growth.The nitrogenous fertilizer largely consumed is to feeding
It is expensive for growing for microalgae, if nitrogenous fertilizer can be provided for micro algae growth using the NOx in industrial waste gas, on the one hand can be reduced
The cost of cultivating microalgae；On the other hand the discharge that exhaust gas can be purified again, reduce NOx generates more overall situation benefit.
In general, photoautotrophic efficiency is less than 30g.m-2.d-1, the efficiency of outdoor large-scale culture is generally below 10g.m- 2.d-1, a large amount of soil can be occupied by carrying out industrial waste gas denitration with such efficiency, it is therefore necessary to further increase microalgae
Breeding efficiency.Addition organic carbon source carries out Heterotrophic culture or luminous energy and supports to be to accelerate the feasible method of micro algae growth, however adding
After adding organic carbon source, algae solution easily by the pollution of harmful bacteria, causes the growth of bacterium to be significantly faster than the growth of microalgae, thus
Both culturing microalgae is caused to fail.
Summary of the invention
The present invention provides a kind of method of cultivating microalgae, this method by being added a certain amount of EM in the breeding process
Bacterium can either promote the growth of microalgae, and can enhance the ability that microalgae resists miscellaneous bacteria and pest and disease damage, therefore be more suitable for extensive
Cultivating microalgae.
A kind of method of cultivating microalgae, which is characterized in that in breeding process, EM bacterium is added into algae solution.
Micro algae growth needs necessary condition, such as suitable temperature, sufficient illumination, enough water, CO2And nitrogen
The nutriments such as fertilizer, phosphate fertilizer, regulate and control that dissolved oxygen in algae solution, pH value is in suitable range etc..Although for different microalgaes,
These conditions are not quite similar, but these are all known in the art.
In general, cultivation temperature is 15~40 DEG C, preferable temperature is 25~35 DEG C；Light intensity is 1000~200000 Le
Ke Si, preferable light intensity are 5000~150000 luxs；Algae solution pH value is 6~11, and preferable algae solution pH value is 7~9.
According to the present invention, breeding way can be photoautotrophy (under light illumination, merely with inorganic carbon source such as CO2It is raw
It is long), Heterotrophic culture (Heterotrophic culture refers to be grown merely with organic carbon source) or luminous energy and support that (luminous energy and supporting refers to, under light illumination
Inorganic carbon source such as CO is utilized simultaneously2It is grown with organic carbon source).
According to the present invention, there is no limit as long as the microalgae is suitable for supporting using selected type of the present invention to microalgae
Grow mode.The present invention preferably cultivates those microalgaes for being suitable for oil-producing, to obtain bioenergy.
It is known in the art that needing to provide organic carbon source to microalgae when using Heterotrophic culture or luminous energy and the breeding way supported.
According to the present invention, available organic carbon source includes but is not limited to sugar, organic acid, acylate, alcohol, cellulose hydrolysate and starch
At least one of hydrolysate；For example it can be selected from glucose, fructose, acetic acid, sodium acetate, lactic acid, ethyl alcohol, methanol, cellulose water
At least one of object and cellulose hydrolysate are solved, preferably selection is glucose.
It is known in the art that with the continuous growth of micro algae biomass, need according in algae solution micro algae biomass and nutrition
The insufficient nutriment of content is replenished in time in content of material.According to the present invention, any mode for adding nutriment is all available
, for example be segmented and add or continuously add, as long as the amount of nutriment can be controlled in reasonable range.
When according to the present invention, using Heterotrophic culture or luminous energy and the breeding way supported, generally by the concentration control of organic carbon source
System is preferably controlled in 2g/L algae solution~10g/L algae solution in 1g/L algae solution~30g/L algae solution.
Although luminous energy and feeding or Heterotrophic culture can increase part aquaculture cost, breeding efficiency because using organic carbon source
Also it greatly improves, is simplified following process process, so if can be avoided sterile cultivation, it will be able to avoid consumption a large amount of
Steam carries out stringent sterilization processing to system, so that aquaculture cost be greatly reduced.According to the present invention, particularly preferably those can luminous energy
And feeding or Heterotrophic culture microalgae, such as chlorella, scenedesmus, single needle algae or spirulina., it is surprising that with luminous energy and support or
When these microalgaes of Heterotrophic culture mode, as long as a certain number of EM bacterium are added, disinfecting action, cultivation are not carried out to culture environment
It can go on smoothly, the growth rate of microalgae is greatly speeded up, even if water source contains a large amount of harmful bacterias and/or opens wide cultivation, as a result
It is also such；And when being added without EM bacterium, if not carrying out disinfecting action to culture environment, luminous energy and feeding or Heterotrophic culture would generally
Failure.
According to the present invention, it the Heterotrophic culture or luminous energy and supports, without disinfecting action.
According to the present invention, in the breeding process, preferably with NO3 -And/or NO2 -As nitrogen source, particularly preferably with industry
Exhaust gas denitration salt made from earth containing a comparatively high percentage of sodium chloride obtained and/or nitrosonium salts are as nitrogen source, such as with alkali absorption method salt made from earth containing a comparatively high percentage of sodium chloride obtained and/or nitrous
Salt or with nitric acid absorption process through alkali neutralization salt made from earth containing a comparatively high percentage of sodium chloride obtained and/or nitrosonium salts.It is added in the breeding process a certain number of
After EM bacterium, the growth of microalgae is not only accelerated, to inorganic nitrogen-sourced such as NO3 -And/or NO2 -Wear rate also increase
Add, the present invention made to be quite suitable for industrial waste gas denitration, can use the fixed salt of NOx on the one hand as micro algae growth and nitrogenous fertilizer is provided,
To reduce the cost of cultivating microalgae；On the other hand the discharge for being conducive to purifying industrial waste gases again, reducing NOx.
According to the present invention, specific microalgae, such as chlorella, scenedesmus, single needle algae or spirulina, they can be metabolized simultaneously
NO3-And NO2-, it can be resistant to the environment of high nitrogen concentration, cultivate the discharge that these microalgaes are more advantageous to purification exhaust gas, reduce NOx.
According to the present invention, it when photoautotrophy or luminous energy are simultaneous feeding, needs to provide inorganic carbon source, such as CO to microalgae2.When with
NO3 -And/or NO2 -When as nitrogen source, CO is contained by control2Gas intake, the pH value of algae solution is controlled with can be convenient
In suitable range.
The EM bacterium (Effective Microorganisms) belongs to the prior art, mainly by belonging to photosynthetic bacteria
Group, lactobacillus, yeast flora, Gram positive actinomycetes group, fermentation system Filamentous flora tens kinds of microorganism groups at being
A kind of commercially available active bacteria formulation.The EM bacterium both can voluntarily prepare according to existing knowledge, can also be commercially available, and use
It is preceding to need to illustrate to ferment according to having knowledge or commercial preparations.
In accordance with the present invention, it was found that there are two types of functions for EM bacterium tool, first is that can promote the growth of microalgae；Second is that can inhibit to microalgae
Harmful bacterial reproduction.It should be appreciated that the purpose of the present invention is obtain microalgae biomass, therefore the dosage of EM bacterium should meet plus
The needs of fast micro algae growth can not neither work because dosage is very few, and cannot compete and consume with microalgae due to dosage is excessive
Excessive nutriment.The adding manner (for example be added at one time or be added several times) and any EM bacterium dosage of any EM bacterium
It is all available, as long as being able to satisfy the needs for accelerating micro algae growth.
According to the present invention, the additional amount of EM bacterium is preferably 1 × 106A/algae solution~9 × 10 L8A/L algae solution；More preferably 1
×107A/algae solution~5 × 10 L8A/L algae solution.
The present invention also provides a kind of methods of industrial waste gas denitration, which is characterized in that with the method for above-mentioned cultivating microalgae
NOx absorbing liquid of the generated feeding algae raffinate as industrial waste gas denitration.
Preferably, alkalinity caused by the method for above-mentioned cultivating microalgae is supported algae raffinate and is inhaled as the NOx of industrial waste gas denitration
Receive liquid.The pH value of the feeding algae raffinate of alkalinity is preferably 9~11.
Make us unexpected, compared with the lye of preparation, alkalinity caused by the method for aforementioned cultivating microalgae supports algae
Raffinate has higher absorption efficiency to the NOx in industrial waste gas.
The present invention achieves following technical effect.
According to the present invention, no matter which kind of breeding way is used, as long as EM bacterium is added in algae solution, it will be able to effectively inhibit
The breeding of harmful bacteria increases substantially the growth rate of microalgae.This feature makes the present invention be more suitable for large-scale farming microalgae,
Especially when Heterotrophic culture or luminous energy are simultaneous feeding, due to not needing the sterilizing that carries out disinfection, make the present invention with greater advantage.
According to the present invention, after EM bacterium being added in algae solution, microalgae is inorganic nitrogen-sourced with high efficiency consumption, makes the present invention ten
Divide and is suitable for industrial waste gas denitration.
According to the present invention, specific microalgae, such as chlorella, scenedesmus, single needle algae or spirulina, they can be metabolized simultaneously
NO3-And NO2-, it can be resistant to the environment of high nitrogen concentration, cultivate the discharge that these microalgaes are more advantageous to purification exhaust gas, reduce NOx.
According to the present invention, compared with the lye of preparation, the feeding algae raffinate of alkalinity has higher suction to the NOx in industrial waste gas
It produces effects rate.
Unless otherwise defined, all technical and scientific terms used herein all has those skilled in the art conventional
The meaning of understanding.In case of conflict, it is subject to the definition of this specification.
In the context of the present specification, other than the content clearly stated, any matters or item that do not mention are equal
It is directly applicable in those of known in the art without carrying out any change.
All features disclosed in this invention can in any combination, these combinations should be understood in disclosed in this invention
Hold, unless those skilled in the art think that the combination is obviously unreasonable；Numerical point disclosed in this specification (including each numerical value model
The endpoint enclosed) range of institute's any combination is regarded as the published range of the present invention, no matter whether disclosing one by one herein
These numerical value pair.
Detailed description of the invention
Fig. 1 is photoautotrophic micro algae growth curve.
Fig. 2 is luminous energy and feeding micro algae growth curve.
Fig. 3 is using salt made from earth containing a comparatively high percentage of sodium chloride as the micro algae growth curve of nitrogen source.
Fig. 4, Fig. 5 are the micro algae growth curve for adding a large amount of organic carbon sources.
Fig. 6 is the micro algae growth curve of addition EM bacterium under conditions of unglazed heterotrophism.
Fig. 7 is the schematic diagram of NOx absorption technique.
Fig. 8 is NOx absorptivity versus time curve.
Specific embodiment
With embodiment, the present invention will be described in detail below, but is not therefore construed as limiting the invention.
Algae solution OD value (OD680Value) measurement: OD value spectrophotometric determination is compared with distilled water, measurement
Light absorption value of the algae solution at wavelength 680nm, the index as microalgae concentration.
The measurement of solution nitrogen content: using in ICS3000 type ion chromatograph (Dionex company, the U.S.) measurement aqueous solution
NO3Content or NO2 -Content, instrument is equipped with EG40 leacheate automatic generator, electric conductivity detector and the work of chameleon chromatography
It stands；IonPac AS11-HC type splitter (250mm × 4mm i.d.)；IonPac AG11 type guard column (50mm × 4mm
i.d.)；Itself suppressor of ASRS-ULTRA anion.Leacheate: KOH solution；Flow velocity is 1mL/min；Eluent concentration:
30mmol/L；Sample volume is 60 μ L；Column temperature is 30 DEG C；Inhibit electric current 100mA；External standard method peak area quantification.
Count of bacteria: count of bacteria is carried out according to the following steps
1. sample washs: drawing 1ml sample, washed 2-3 times with 1 × PBS；2. initial gross separation: according to algae and bacterium from
The difference of mental and physical efforts is centrifuged 2min, initial gross separation algae with 1000rpm first (for bacterium in supernatant, algae is in precipitating)；If
When algae content is higher, repeat again；3. collecting supernatant, the amount of algae in supernatant is negligible at this time, 8000rpm centrifugation
5min abandons supernatant；4. precipitating is resuspended with the rupture of membranes agent of 500ul bacterium, 15min is reacted at room temperature；5.8000rpm is centrifuged 5min, with 1 ×
PBS washs 2 bacterium solutions；6. 100ul1 × PBS, which is added, is resuspended thallus, 5ul PI dye liquor mother liquor is added, reacts at room temperature 30min；7.
Fluorescence microscopy microscopic observation bacterium simultaneously counts, and bacterial number is up to 1000 in 4 block plaids, when being greater than 1000, dilution
Bacterium solution certain multiple counts again；8. calculation formula:
Bacterial density=count results/4 × extension rate × 4 × 10 in surveyed solution4A/ml
Main agents consumptive material:
| Agents useful for same consumptive material | Manufacturer |
| PI Viability Staining Solution | Four positive cypress Cat No.FXP002 |
| Rupture of membranes agent | Sharp that health Cat No.REK3004 |
| Phosphate buffer (10 × PBS, pH7.4, cell culture grade are sterile) | Sharp that health Cat No.REK3013 |
| Cell climbing sheet | NEST |
Key instrument:
| Instrument | Manufacturer |
| Tally | Shanghai precision instrument |
| Fluorescence microscope | Olympus BX-51 |
The culture medium of microalgae: medium component is shown in Table 1~table 2.
1 culture medium BG11 of table
| Component | Composition, mg/L |
| K2HPO4·3H2O | 40 |
| NaNO3 | 1500 |
| Na2CO3 | 20 |
| MgSO4·7H2O | 75 |
| CaCl2·2H2O | 36 |
| Citric acid | 6 |
| Ferric citrate | 6 |
| EDETATE SODIUM | 1 |
| Microelement A5 | 1 |
2 microelement A5 of table
| Component | Composition, mg/L |
| H3BO3 | 2860 |
| MnCl2·4H2O | 1810 |
| ZnSO4·7H2O | 222 |
| CuSO4·5H2O | 79 |
| NaMoO4·5H2O | 390 |
| Co(NO3)2·6H2O | 50 |
3 Heterotrophic culture base of table
4 microelement of table
| Component | Composition, g/L |
| H3BO3 | 2.86 |
| MnCl2·4H2O | 0.11 |
| ZnSO4·7H2O | 9.22 |
| CuSO4·5H2O | 1 |
| (NH4)Mo7O24·4H2O | 0.1 |
| Co(NO3)2·6H2O | 0.9 |
EM bacterium: probiotics used in embodiment is the production of the oasis Kang Yuan Biotechnology Co., Ltd such as golden probiotics,
Illustrate to carry out activation processing, PH < 4 by it using preceding.
Embodiment 1
The present embodiment is for illustrating " addition EM bacterium is on the photoautotrophic influence of microalgae ".
It (is come from using BG11 culture medium (adding nutritional ingredient by table 1, culture solution is without sterilization treatment) culture chlorella
Sinopec microalgae algae library, number Chlorella sp.RIPP-1), between 20~30 DEG C, it is empty to be passed through compression
Gas and CO2Culture, is passed through CO as algae solution PH > 102, stop being passed through CO as algae solution PH < 7.52.Nature is used in incubation
Day optical culture, daylight intensity reach as high as 60000 luxs, detect the OD of algae solution daily680Value, after continuous culture 14 days
Harvest, culture terminate to stop within first 1 day being passed through containing CO2Gaseous mixture, terminate cultivation after, by being centrifugally separating to obtain algal gel and feeding algae
Raffinate.The growth curve of microalgae is shown in Fig. 1, and two in Fig. 1 test difference and are only that: EM bacterium is not added in one of test,
In another test press 3.6 × 106The additive amount of a/L algae solution adds EM bacterium.For adding the test of EM bacterium, in breeding process
Monitor count of bacteria < 6.7 × 10 of algae solution6A/mL algae solution, algae solution pH is increased to 9.8 naturally at the end of measuring cultivation.From Fig. 1
In as it can be seen that under the conditions of photoautotrophy, addition EM bacterium promotes the growth of microalgae.
Embodiment 2~5 for illustrate " luminous energy and support in, influence of the EM bacterium additive amount to microdisk electrode ".
Embodiment 2
It (is come from using BG11 culture medium (adding nutritional ingredient by table 1, culture solution is without sterilization treatment) culture chlorella
Sinopec microalgae algae library, number Chlorella sp.RIPP-1), the glucose of 2g/L is added in incubation, controls temperature
Between 20~30 DEG C, it is passed through compressed air and CO2Culture, is passed through CO as algae solution PH > 102, stop as algae solution PH < 7.5 logical
Enter CO2.Natural daylight culture is used in incubation, daylight intensity reaches as high as 60000 luxs, detects algae solution daily
OD680Value, the growth curve of microalgae are shown in Fig. 2.Wherein EM additive amount is 3.6 × 106A/L algae solution monitors algae in breeding process
Count of bacteria < 8 × 10 of liquid6A/mL algae solution, continuous culture harvest after 14 days, and culture terminates to stop within first 1 day being passed through CO2Flue gas,
And algae solution pH is made to be increased to 9.4 naturally, then terminate to cultivate, is centrifugally separating to obtain algal gel and feeding algae raffinate.
Embodiment 3
The present embodiment and the difference of embodiment 2 are only that: EM additive amount is 1.8 × 107A/L algae solution.It is supervised in breeding process
Survey count of bacteria < 1 × 10 of algae solution7A/mL algae solution, the pH for measuring algae solution at the end of cultivating are increased to 9.3 naturally.The life of microalgae
Long curve is shown in Fig. 2.
Embodiment 4
The present embodiment and the difference of embodiment 2 are only that: EM additive amount is 3.6 × 107A/L algae solution.It is supervised in breeding process
Survey count of bacteria < 2 × 10 of algae solution7A/mL algae solution, the pH for measuring algae solution at the end of cultivating are increased to 8.9 naturally.The life of microalgae
Long curve is shown in Fig. 2.
Embodiment 5
The present embodiment and the difference of embodiment 2 are only that: EM additive amount is 7.2 × 107A/L algae solution.It is supervised in breeding process
Survey count of bacteria < 5.8 × 10 of algae solution7A/mL algae solution, the pH for measuring algae solution at the end of cultivating are increased to 8.7 naturally.Microalgae
Growth curve is shown in Fig. 2.
Comparative example 1
The difference of this comparative example and embodiment 2 is only that: not adding EM bacterium.The count of bacteria of algae solution is monitored in breeding process
It has been up to 1.2 × 108A/mL algae solution, the pH for measuring algae solution at the end of cultivating are increased to 7.9 naturally.The growth of microalgae is bent
Line is shown in Fig. 2.
It can be seen in fig. 2 that addition EM bacterium promotes the growth of microalgae under the conditions of luminous energy is simultaneous feeding.
Embodiment 6~8 is for illustrating " metabolism of the microalgae to NO3-N and NO2-N ".
Embodiment 6
It (is come from using BG11 culture medium (adding nutritional ingredient by table 1, culture solution is without sterilization treatment) culture chlorella
Sinopec microalgae algae library, number Chlorella sp.RIPP-1), between 20~30 DEG C, it is empty to be passed through compression
Gas and CO2Culture, is passed through CO as algae solution PH > 102, stop being passed through CO as algae solution PH < 7.52.Nature is used in incubation
Day optical culture, daylight intensity reach as high as 60000 luxs, detect the OD of algae solution daily680Value, continuous culture 14 days.It is micro-
The growth curve of algae is shown in Fig. 3.
Embodiment 7
The present embodiment and the difference of embodiment 6 are only that: the sodium nitrate of 1.5g/L in culture medium is substituted for 1.35g nitrous
Sour sodium and 0.15g sodium nitrate.The growth curve of microalgae is shown in Fig. 3.
Embodiment 8
The present embodiment and the difference of embodiment 7 are only that: culture microalgae is that single needle algae (comes from Sinopec microalgae algae
Library, number Monoraphidium dybowskii.RIPP-50).The growth curve of microalgae is shown in Fig. 3.
It can be seen from figure 3 that using the microalgae algae of institute's breeding, it can be preferably raw using NO3-N and NO2-N simultaneously
It is long.
Embodiment 9~16 is for illustrating that " in the case where largely addition organic carbon source, EM bacterium is metabolized microalgae inorganic nitrogen-sourced
Influence ".
Embodiment 9
First using BG11 culture medium (adding nutritional ingredient by table 1, culture solution is without sterilization treatment) culture chlorella
(coming from Sinopec microalgae algae library, number Chlorella sp.RIPP-1)；Work as OD680When value is 4, mended by 3 specified amount of table
Add a Heterotrophic culture base nutritional ingredient.Between 20~30 DEG C, it is passed through compressed air and CO2Culture, works as algae solution
CO is passed through when PH > 102, stop being passed through CO as algae solution PH < 7.52.Natural daylight culture is used in incubation, daylight is strong
Degree reaches as high as 60000 luxs, adds the glucose of 2g/L, and press 2.9 × 107The amount of a/L algae solution adds EM bacterium, daily
Detect the OD of algae solution680Value；The glucose of 10g/L is added in culture again after 1 day, and presses 3.6 × 107A/L algae solution adds EM bacterium；
Glucose 10g/L is added when culture was to the 5th day again, monitoring the count of bacteria of algae solution in breeding process is up to 9.7 × 106
A/mL algae solution, continuous culture harvest after 8 days, and last time stops being passed through CO after glucose is added2, terminate algae solution PH when cultivation
Value is 8.6, is centrifugally separating to obtain algal gel and feeding algae raffinate.Analyze the NO supported in algae raffinate3With NO2Total content < 10 μ g/g.
The growth curve of microalgae is shown in Fig. 4.
Embodiment 10
The present embodiment and the difference of embodiment 9 are only that: culture microalgae is that single needle algae (comes from Sinopec microalgae algae
Library, number Monoraphidium dybowskii.RIPP-50).The count of bacteria that algae solution is monitored in breeding process is up to
4.6 × 107A/mL algae solution, the pH for measuring algae solution at the end of cultivating are increased to 8.2 naturally, analyze the NO supported in algae raffinate3With
NO2Total content < 200 μ g/g.The growth curve of microalgae is shown in Fig. 4.
Embodiment 11
The difference of the present embodiment and embodiment 9 is only that following aspect: the EM bacterium additive amount of first time is 7.9 × 107A/
L algae solution does not add secondary EM bacterium；And second of glucose amount added is 30g/L, does not add third time glucose.
It is up to 2.6 × 10 that the count of bacteria of algae solution is monitored in breeding process7A/mL algae solution measures the pH of algae solution at the end of cultivating certainly
8.2 are so increased to, the NO supported in algae raffinate is analyzed3With NO2Total content < 10 μ g/g.The growth curve of microalgae is shown in Fig. 4.
Embodiment 12
The present embodiment and the difference of embodiment 11 are only that: culture microalgae is that single needle algae (comes from Sinopec microalgae algae
Library, number Monoraphidium dybowskii.RIPP-50).The count of bacteria that algae solution is monitored in breeding process is up to
5.2 × 107A/mL algae solution, the pH for measuring algae solution at the end of cultivating are increased to 7.8 naturally, analyze the NO supported in algae raffinate3With
NO2Total content < 200 μ g/g.The growth curve of microalgae is shown in Fig. 4.
Comparative example 2
The difference of this comparative example and embodiment 9 is only that: not adding EM bacterium.Monitor incubation in algae solution count of bacteria most
A height of 13.6 × 108A/mL algae solution, the pH for measuring algae solution at the end of cultivating are increased to 7.2 naturally.The growth curve of microalgae is shown in figure
4。
As can be seen from Fig. 4, addition EM bacterium is greatly facilitated the growth of microalgae and consumes rapidly inorganic nitrogen-sourced.
Embodiment 13
First using BG11 culture medium (adding nutritional ingredient by table 1, culture solution is without sterilization treatment) culture chlorella；
Work as OD680When value is 4, a Heterotrophic culture base nutritional ingredient is added by 3 specified amount of table.Between 20~30 DEG C, lead to
Enter compressed air and CO2Culture, is passed through CO as algae solution PH > 102, stop being passed through CO as algae solution PH < 7.52.In incubation
Using natural daylight culture, daylight intensity reaches as high as 60000 luxs, first in illumination autotrophy item after chlorella inoculation
It is cultivated under part 2 days, then adds the glucose of 2g/L, and press 1.8 × 108The amount of a/L algae solution adds EM bacterium, detects algae daily
The OD of liquid680Value；The glucose of 10g/L is added in culture again after 3 days, and presses 1.8 × 108A/L algae solution adds EM bacterium；Culture 2
Glucose 10g/L is added after it again, monitoring the count of bacteria of algae solution in breeding process is up to 2.9 × 107A/mL algae solution,
Continuous culture harvests after 14 days, and last time stops being passed through CO after glucose is added2, terminating algae solution pH value when cultivation is 9.2, from
The isolated algal gel of the heart and feeding algae raffinate.Analyze the NO supported in algae raffinate3With NO2Total content < 10 μ g/g.The growth of microalgae
Curve is shown in Fig. 5.
Embodiment 14
The difference of the present embodiment and embodiment 13 is only that following aspect: not adding secondary EM bacterium；And second
The glucose amount of addition is 30g/L, does not add third time glucose.In breeding process monitor algae solution count of bacteria be up to
2.9×107A/mL algae solution, the pH for measuring algae solution at the end of cultivating are increased to 9.3 naturally, analyze the NO supported in algae raffinate3With
NO2Total content < 10 μ g/g.The growth curve of microalgae is shown in Fig. 5.
Embodiment 15
The present embodiment and the difference of embodiment 13 are only that: NaNO in BG11 culture medium3Replace with KNO3, and KNO3Add
Dosage is 0.5g/L.It is up to 1.3 × 10 that the count of bacteria of algae solution is monitored in breeding process7A/mL algae solution measures end cultivation
When algae solution pH value be 9.4, analyze support algae raffinate in NO3With NO2Total content < 10 μ g/g.The growth curve of microalgae is shown in figure
5。
Embodiment 16
The present embodiment and the difference of embodiment 14 are only that: the NaNO in BG11 culture medium3Replace with KNO3, and KNO3
Additive amount be 0.5g/L.It is up to 1.7 × 10 that the count of bacteria of algae solution is monitored in breeding process7A/mL algae solution, measures end
The pH value of algae solution is 9.3 when cultivation, analyzes the NO supported in algae raffinate3With NO2Total content < 10 μ g/g.The growth curve of microalgae
See Fig. 5.
As can be seen from Fig. 5, using potassium nitrate or sodium nitrate as nitrogen source, the growth that EM bacterium promotes microalgae is added.
Embodiment 17 is for illustrating " EM bacterium is on microalgae without photoheterotrophic influence ".
Embodiment 17
The present embodiment and the difference of embodiment 9 are only that: being cultivated under no light condition.Measure the pH of algae solution when terminating cultivation
Value is 7.7.The growth curve of microalgae is shown in Fig. 6.
Embodiment 18 is for illustrating " absorbing NOx with algae raffinate is supported ".
Embodiment 18
Using O3Auxiliary law absorbs NOx.
Using NO2Practical flue gas is simulated with the gaseous mixture of NO, using compressed air as carrier gas, NOx flow is 0.3L/min, is contained
O3The mobile ozone generator of XM-Y type that produces from Qingdao Xin Mei cleaning equipment Co., Ltd of gas, flow 1L/min,
Make total flow up to 150L/h after mixing air, measures the NOx concentration of entrance and exit gas, NOx absorptivity is calculated as follows；
NOx absorptivity=(1- exit NOx concentration/inlet NOx concentration) × 100%；
Wherein the total concentration of entrance NOx is basically stable at 620mg/m3(wherein NO content is about 600mg/m3, NO2Content is about
For 20mg/m3)
Flow chart is shown in Fig. 7, wherein absorption tower diameter 100mm, high 700mm, and tower bottom is equipped with sieve-mesh gas distributor,
In hold 3L embodiment 14 generation feeding algae raffinate.NOx mixed gas is passed directly into absorption tower when operation, 22h is absorbed and stops
Operation, NOx absorption efficiency curve are shown in Fig. 8.
Comparative example 3
This comparative example is used to illustrate " absorbing NOx using the lye prepared ".
Potassium, the Na ion concentration in the feeding algae raffinate (pH value 9.3) that embodiment 14 generates are analyzed, is prepared with it with identical
The aqueous solution 3L of potassium concentration and Na ion concentration, pairing anion are HCO3 -And CO3 2-, prepared pH value of water solution is
9.27, the pH value for supporting algae raffinate with the alkalinity of embodiment 14 is essentially identical.Algae raffinate and preparation are supported with above-mentioned alkalinity respectively
Aqueous solution is absorbing liquid, absorbs NOx using the method for embodiment 18, sees Fig. 8 to the absorption efficiency curve of NOx.
As seen from Figure 8, the lye that algae raffinate is apparently higher than preparation to the absorptivity of NOx is supported.
Claims (9)
1. a kind of method of cultivating microalgae, which is characterized in that in breeding process, EM bacterium is added into algae solution；The additional amount of EM bacterium
It is 1 × 106A/algae solution~9 × 10 L8A/L algae solution；The microalgae is chlorella, scenedesmus, single needle algae or spirulina；Cultivation side
Formula is Heterotrophic culture or luminous energy and supports；It the Heterotrophic culture or luminous energy and supports, without disinfecting action.
2. the method according to claim 1, wherein organic carbon source used be selected from glucose, fructose, acetic acid,
At least one of sodium acetate, lactic acid, ethyl alcohol, methanol and cellulose hydrolysate.
3. the method according to claim 1, wherein controlling the concentration of organic carbon source used in 1g/L algae solution
~30g/L algae solution.
4. the method according to claim 1, wherein in the breeding process, with NO3 -And/or NO2 -As nitrogen
Source.
5. according to the method described in claim 4, it is characterized in that, being obtained in the breeding process with industrial waste gas denitration
The salt made from earth containing a comparatively high percentage of sodium chloride and/or nitrosonium salts obtained is as nitrogen source.
6. the method according to claim 1, wherein the additional amount of EM bacterium is 1 × 107A/algae solution~5 × 10 L8
A/L algae solution.
7. cultivation temperature is 15~40 the method according to claim 1, wherein breeding way is luminous energy and supports
DEG C, light intensity is 1000~200000 luxs, and algae solution pH value is 6~11.
8. a kind of method of industrial waste gas denitration, which is characterized in that support algae caused by any one of claim 1~7
NOx absorbing liquid of the raffinate as industrial waste gas denitration.
9. according to the method described in claim 8, it is characterized in that, the alkalinity caused by any one of claim 1~7
Support NOx absorbing liquid of the algae raffinate as industrial waste gas denitration.
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| CN106635919A (en) * | 2017-01-16 | 2017-05-10 | 江西中藻生物科技股份有限公司 | Spirulina culture method |
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