CN105303015B - Optimize the method and device of contaminated site soil ecology toxicity diagnostic result - Google Patents
Optimize the method and device of contaminated site soil ecology toxicity diagnostic result Download PDFInfo
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Abstract
The invention discloses a kind of method and device for optimizing contaminated site soil ecology toxicity diagnostic result.This method includes：Soil ecology toxicity diagnostic result, inquiry poisonous effect and pollution evaluation mapping table are gathered, obtains the pollution assignment of poisonous effect mapping corresponding to soil ecology toxicity diagnostic result；Kind of test according to belonging to toxicity test classification chart obtains diagnostic test, inquiry kind of test and experiment weight coefficient mapping table, obtain the experiment weight coefficient of affiliated kind of test mapping；The Testing index that diagnostic test uses is determined, inquires about the mapping table of Testing index and index weights, obtains the index weights of the Testing index mapping of use；Soil ecology toxicity integrated integral is obtained according to the pollution assignment of acquisition, experiment weight coefficient and index weights, and calculates the ratio with individual level integration maximum, the soil ecology Severity level of contaminated site is determined according to ratio.Using the present invention, the accuracy that contaminated site is assessed can be effectively lifted.
Description
Technical field
The present invention relates to soil ecology technology, more particularly to a kind of optimization contaminated site soil ecology toxicity diagnostic result
Method and device.
Background technology
Soil pollution is one of worldwide environmental problem, and cleaning and the safe utilization of contaminated soil are a vital tasks,
Soil pollution diagnosis is one of important step.Soil pollution diagnosis is carried out by chemical method, it is impossible to comprehensive, section merely
The total quality characteristic that ground characterizes soil is learned, following limitation be present：(1) various pollutants in soil can not be surveyed comprehensively
It is fixed, it is impossible to identify the poisonous effect of all genotoxic potential materials, can not also determine the combined pollution effect of pollutant；(2) only with
Pollutant load can not react poisonous effect of these pollutants to biology；(3) it is difficult to distinguish different route of exposure (such as spaces
Water, soil air etc.) in polluter concentration, the effective toxicity of pollutant may be underestimated；(4) can not be to the generation of pollutant
Thank to toxicity to be tracked.Therefore other methods are needed to make supplement to this.It is combined with chemical analysis and eco-toxicological indicator
Method, can the more evaluation soil environment sample of science overall toxicity and environmental hazard.By selecting in soil environment
The sensitive representative of Different Nutrition level completes serial ecotoxicological experiment, used as the index diagnosed to the actual toxicity of pollutant
This pollution toxicity diagnosis index system formed plays an important roll to soil pollution diagnosis.
At present, in order to obtain the detailed polluted information of contaminated site, with according to the polluted information of acquisition to contaminated site
Pollution situation is assessed, and typically the soil ecology toxicity of contaminated site is carried out using comprehensive, all standing diagnostic test
Diagnosis, diagnostic test include：Bio-toxicity diagnostic test and genetoxic diagnostic test, wherein,
Bio-toxicity diagnostic test includes：Soil extract is tested and the experiment of actual soil original soil, actual soil original soil
Experiment includes again：In short term/acute toxicity test and long-term/chronic toxicity test.Wherein, soil extract experiment includes aquatic
Biologic test, short-term/acute toxicity test include plant it is short-term/acute toxicity test and geobiont (animal, microorganism)
In short term/acute toxicity test, long-term/chronic toxicity test include plant it is long-term/chronic toxicity test and soil animal it is long-term/
Chronic toxicity test.
Genetic toxicity test includes：Micronucleus test, mutagenicity test, DNA damage experiment etc..
In practical application, because soil ecosystem is a complicated system, different biological subjects is to soil ecology
The influence of system is different, meanwhile, the Testing index of selection is also different to the sensitiveness of the concern pollutant in contaminated site so that
Different biological subject, carry out for different Testing index soil ecology toxicity diagnostic result difference that diagnostic test obtains compared with
Greatly, and in the prior art, it is general using choosing maximum or malicious using final soil ecology is obtained by the way of equalization weights
Property diagnostic result, as contaminated site pollution situation assess foundation so that the accuracy of assessment is not high, easily by biological subject
And the influence for the Testing index chosen, it is impossible to reflect the actual pollution situation of contaminated site.
The content of the invention
In view of this, it is a primary object of the present invention to propose a kind of optimization contaminated site soil ecology toxicity diagnostic result
Method and device, lifting contaminated site assess accuracy.
To reach above-mentioned purpose, the invention provides a kind of side for optimizing contaminated site soil ecology toxicity diagnostic result
Method, this method include：
The soil ecology toxicity diagnostic result directly obtained by diagnostic test is gathered, inquires about the poisonous effect pre-set
With polluting evaluation mapping table, the pollution assignment that poisonous effect corresponding to the soil ecology toxicity diagnostic result maps is obtained；
According to the toxicity test classification chart pre-set, the kind of test belonging to diagnostic test is obtained, inquiry is pre-set
Kind of test with experiment weight coefficient mapping table, belonging to the diagnostic test obtained kind of test mapping experiment weight
Coefficient；
The Testing index that diagnostic test uses is determined, inquires about the mapping relations of the Testing index pre-set and index weights
Table, obtain the index weights for the Testing index mapping that the diagnostic test uses；
The soil ecology toxicity that the pollution assignment of acquisition, experiment weight coefficient and index weights are applied to pre-set
Integrated integral formula, obtain soil ecology toxicity integrated integral；
Soil ecology toxicity integrated integral and the ratio of individual level integration maximum are calculated, and it is true according to the ratio of calculating
Determine the soil ecology Severity level of contaminated site.
It is preferred that the poisonous effect includes with pollution evaluation mapping table：
If poisonous effect is more than 80%, the levels of contamination for determining soil ecology toxicity diagnostic result is serious pollution, if
Put pollution and be entered as 5；
If poisonous effect is more than 50% and less than or equal to 80%, the pollution level of soil ecology toxicity diagnostic result is determined
Not Wei serious pollution, set pollution be entered as 4；
If poisonous effect is more than 30% and less than or equal to 50%, the pollution level of soil ecology toxicity diagnostic result is determined
Not Wei intermediate pollution, set pollution be entered as 3；
If poisonous effect is more than 15% and less than or equal to 30%, the pollution level of soil ecology toxicity diagnostic result is determined
Not Wei slight pollution, set pollution be entered as 2；
If poisonous effect is more than 5% and less than or equal to 15%, the pollution level of soil ecology toxicity diagnostic result is determined
Not Wei potential pollution, set pollution be entered as 1；
If poisonous effect is less than or equal to 5%, the levels of contamination of soil ecology toxicity diagnostic result is determined to be pollution-free,
Pollution is set to be entered as 0.
It is preferred that the diagnostic test includes：In short term/acute toxicity test and chronic/genetic toxicity test, wherein,
Chronic/genetic toxicity test includes：For a long time/chronic toxicity test and genetic toxicity test.
It is preferred that short-term/the acute toxicity test is categorized as：One kind experiment and the experiment of two classes, the chronic/heredity
The corresponding three classes experiment of toxicity test, wherein,
For toxic endpoints, one kind experiment includes：Lethal, germination percentage；The experiment of two classes includes：Avoid, be luminous, enzyme activity suppression
System；The experiment of three classes includes：Breeding, development, gene mutation, chromosome aberration；
For Aquatic Toxicity Test, one kind experiment includes algal grown and suppresses experiment；The experiment of two classes includes：Photogen
Acute toxicity test and the acute motility inhibition test of Magna classes；
For terrestrial organism toxicity test, one kind experiment includes：Seed germination experiment, earthworm acute toxicity test；Two classes
Experiment includes：Terrestrial plant growth experiment, plant roots elongation test, plant physiology and biochemistry index determining, earthworm avlidance behavior examination
Test, earthworm Determination of Physiological And Biochemical Indices and microbial respiratory suppress；The experiment of three classes includes：Vegetable active experiment, earthworm breeding
Experiment, enchytrae reproduction test and snail young toxicity test；
For genetic toxicity test, the experiment of three classes includes：Genotoxicity, Ames test and earthworm
Earthworm Comet Assay.
It is preferred that the kind of test includes with experiment weight coefficient mapping table：
The experiment weight coefficient of one kind experiment mapping is 1；
The experiment weight coefficient of two classes experiment mapping is 0.8；
The experiment weight coefficient of three classes experiment mapping is 0.5.
It is preferred that calculate the soil ecology toxicity integrated integral using following formula：
M=A+B+C
In formula,
M is soil ecology toxicity integrated integral；
A is the integrated integral of soil extract toxicity test；
B be terrestrial organism it is short-term/integrated integral of acute toxicity test；
C be terrestrial organism it is long-term/integrated integral of chronic toxicity test.
It is preferred that wherein,
In formula,
n1For the biological subject kind number in soil extract toxicity test；
AiFor in soil extract toxicity test, assignment is polluted corresponding to the poisonous effect of i-th kind of biological subject；
For in soil extract toxicity test, the experiment weight coefficient of i-th kind of affiliated kind of test of biological subject；
For in soil extract toxicity test, the index weights for the Testing index that i-th kind of biological subject uses；
n2For terrestrial organism it is short-term/acute toxicity test in biological subject kind number；
BjFor terrestrial organism it is short-term/acute toxicity test in, pollution assignment corresponding to the poisonous effect of jth kind biological subject；
For terrestrial organism it is short-term/acute toxicity test in, the experiment weight coefficient of the affiliated kind of test of jth kind biological subject；
For terrestrial organism it is short-term/acute toxicity test in, the index weights for the Testing index that jth kind biological subject uses；
n3For terrestrial organism it is long-term/chronic toxicity test in biological subject kind number；
CkFor terrestrial organism it is long-term/chronic toxicity test in, pollution assignment corresponding to the poisonous effect of kth kind biological subject；
For terrestrial organism it is long-term/chronic toxicity test in, the experiment weight coefficient of the affiliated kind of test of kth kind biological subject；
For terrestrial organism it is long-term/chronic toxicity test in, the index weights for the Testing index that kth kind biological subject uses.
It is preferred that calculating the individual level using following formula integrates maximum：
In formula,
Ai maxFor in soil extract toxicity test, greatest contamination assignment corresponding to the poisonous effect of i-th kind of biological subject；
For in soil extract toxicity test, the ceiling effect factor of i-th kind of biological subject；
Bj maxFor terrestrial organism it is short-term/acute toxicity test in, it is maximum dirty corresponding to the poisonous effect of jth kind biological subject
Contaminate assignment；
For terrestrial organism it is short-term/acute toxicity test in, the ceiling effect factor of jth kind biological subject；
Ck maxFor terrestrial organism it is long-term/chronic toxicity test in, it is maximum dirty corresponding to the poisonous effect of kth kind biological subject
Contaminate assignment；
For terrestrial organism it is long-term/chronic toxicity test in, the ceiling effect factor of kth kind biological subject；
0.6 is the index weights tested when Testing index is individual level.
It is preferred that determine the determination soil ecology Severity level using following formula：
In formula,
R is tested soil ecology harmfulness score value；
If R >=0.8, level is endangered to be high；
If 0.5≤R ＜ 0.8, level is endangered for height；
If 0.3≤R ＜ 0.5, level is endangered in being；
It is less harmful level if R ＜ 0.3.
Present invention also offers a kind of device for optimizing contaminated site soil ecology toxicity diagnostic result, the device includes：
Assignment acquisition module is polluted, for gathering the soil ecology toxicity diagnostic result directly obtained by diagnostic test,
The poisonous effect and pollution evaluation mapping table pre-set is inquired about, toxicity corresponding to the soil ecology toxicity diagnostic result is obtained and imitates
The pollution assignment that should be mapped；
Weight coefficient acquisition module is tested, for according to the toxicity test classification chart pre-set, obtaining diagnostic test institute
The kind of test of category, the kind of test pre-set is inquired about with experiment weight coefficient mapping table, the diagnostic test institute obtained
The experiment weight coefficient of the kind of test mapping of category, the toxicity test classification chart are classified based on toxic endpoints sensitiveness；
Index weights acquisition module, the Testing index used for determining diagnostic test, inquires about the detection pre-set and refers to
The mapping table of mark and index weights, obtain the index weights for the Testing index mapping that the diagnostic test uses；
Soil ecology toxicity integrated integral computing module, for the pollution assignment of acquisition, experiment and to be referred to weight coefficient
Mark weight is applied to the soil ecology toxicity integrated integral formula pre-set, obtains soil ecology toxicity integrated integral；
Severity level evaluation module, for calculating soil ecology toxicity integrated integral and individual level integration maximum
Ratio, and determine according to the ratio of calculating the soil ecology Severity level of contaminated site.
As seen from the above technical solutions, a kind of optimization contaminated site soil ecology toxicity diagnostic result provided by the invention
Method and device, respectively corresponding to soil ecology toxicity diagnostic result poisonous effect set pollution assignment, diagnostic test institute
The kind of test of category sets corresponding experiment weight coefficient, and sets corresponding index weights for each Testing index, based on each examination
Pollution assignment, experiment weight coefficient and index weights calculate soil life corresponding to the soil ecology toxicity diagnostic result for testing to obtain
State toxicity integrated integral, according to individual level integration maximum, soil is carried out to the soil ecology toxicity integrated integral being calculated
Earth ecological hazard grade is assessed so that the accuracy of assessment is high, is not easy the Testing index by indivedual biological subjects and selection
Influence, the actual pollution situation of contaminated site can be reflected.
Brief description of the drawings
Fig. 1 is the method flow schematic diagram that the embodiment of the present invention optimizes contaminated site soil ecology toxicity diagnostic result.
Fig. 2 is the apparatus structure schematic diagram that the embodiment of the present invention optimizes contaminated site soil ecology toxicity diagnostic result.
Embodiment
It is right below in conjunction with the accompanying drawings and the specific embodiments to make the object, technical solutions and advantages of the present invention clearer
The present invention is described in further detail.
The existing method for obtaining contaminated site soil ecology toxicity diagnostic result, general each soil ecology using from acquisition
In toxicity diagnostic result, maximum is chosen, or, each soil ecology toxicity diagnostic result is obtained by the way of equalization weights
Final soil ecology toxicity diagnostic result, the foundation that the pollution situation as contaminated site is assessed so that the accuracy of assessment
It is not high, easily influenceed by biological subject and the Testing index of selection, it is impossible to reflect the actual pollution situation of contaminated site.
Based on the measure of polluter toxicity, accurate evaluation is carried out to soil pollution, and how to be based in diagnostic test not
The soil ecology toxicity diagnostic result that same biological subject and different Testing index is tested to obtain carries out reliable assessment,
It is the important step of Soil Pollution Control.
Soil ecotoxicology is a branch in ecological toxicology subject system, is ecological toxicology and modern soil
Learn the frontier branch of science to be formed that intersects, it is intended to study the various poisonous and harmful factors (polluter) to non-in ecological environment of soil
The detrimental effect and its mechanism of human biological.Specific research contents is mainly poisonous and harmful factor in soil ecosystem, for example,
Toxic compounds are to animal, plant and microorganism in the different life levels such as molecule, cell, organ, individual, population and group
Detrimental effect, so as to disclose influence of the poisonous and harmful factor to the ecosystem in soil ecosystem, so as to be controlled for contaminated site
Reason provides scientific basis.Wherein, different food chains biologies in soil pollution ecotoxicological (toxicity) diagnostic techniques set soil
To the overall poisonous effect of chemicals, the full detail of soil pollution can be provided.Thus, in the research of soil ecotoxicology
In, all it be unable to do without the support of eco-toxicity diagnostic method or technology.
In the embodiment of the present invention, the sensitiveness based on biological subject to diagnostic test, toxic endpoints and Testing index is right
The soil ecology toxicity diagnostic result of acquisition pollutes assignment corresponding to setting, meanwhile, carried out for each experiment in diagnostic test
Classification, experiment weight coefficient (effector) is set for the experiment of classification, shows the class test to the pollution assignment of acquisition
Influence degree, moreover, carrying out comprehensive assessment to the Testing index in class test, weight corresponding to each Testing index is set.This
Sample, the soil ecology that the equivalent soil ecology toxicity diagnostic result of a certain biological subject not only directly obtains with the biological subject are malicious
Property pollution assignment corresponding to diagnostic result it is related, it is also related to the class test of use and the Testing index of use, by right
The equivalent soil ecology toxicity diagnostic result of each biological subject is weighted, and so as to obtain soil ecology toxicity integrated integral, is made
The foundation assessed for the pollution situation of contaminated site, due to having considered the specific diagnostic method and tool of contaminated site use
The influence of body biological subject so that the accuracy of assessment is high, is not easy the shadow of the Testing index by single biological subject and selection
Ring, and the actual pollution situation of contaminated site can be accurately reflected.
Fig. 1 is the method flow schematic diagram that the embodiment of the present invention optimizes contaminated site soil ecology toxicity diagnostic result.Ginseng
See Fig. 1, this method includes：
Step 101, the soil ecology toxicity diagnostic result directly obtained by diagnostic test is gathered, inquires about what is pre-set
Poisonous effect and pollution evaluation mapping table, obtain the pollution that poisonous effect corresponding to the soil ecology toxicity diagnostic result maps and assign
Value；
It is known technology, herein on directly obtaining soil ecology toxicity diagnostic result by diagnostic test in this step
Omit detailed description.
In practical application, when directly obtaining soil ecology toxicity diagnostic result by diagnostic test due to (test group), also
It is provided with the diagnostic test of corresponding control group.Thus, in the embodiment of the present invention, consider the soil ecology toxicity diagnosis of test group
As a result the soil ecology toxicity diagnostic result with control group carries out significant difference comparison, obtains characterizing diagnostic result presence
The poisonous effect of significant difference degree, so that by each soil ecology toxicity diagnostic result directly obtained by diagnostic test with poison
Property effect is characterized.So, can be more accurate because poisonous effect is relatively obtained by the significant difference with control group
Reflect the pollution level of contaminated site.
In the embodiment of the present invention, it is contemplated that the response range of each toxicity test differs.Specifically, it is relatively quick
The index (toxic endpoints sensitiveness) of sense can be strongly inhibited under the toxicity test that low concentration pollutes, so as to lose
To the responding ability of the toxicity test of higher concentration pollution；And the index of relative insensitivity can to the toxicity test of low concentration pollution
Response can not be produced so that the soil ecology toxicity diagnostic result difference for the toxicity test that different indexs pollutes to same concentration
It is larger, or, the soil ecology toxicity diagnostic result difference for the toxicity test that identical index pollutes to various concentrations is larger, from
And influence the accuracy of soil ecology toxicity diagnostic result.Thus, consider to carry out poisonous effect corresponding to different toxicity tests
Assignment is classified, to embody the influence to soil ecology toxicity diagnostic result.
It is (biological compared with control group to poisonous effect corresponding to soil ecology toxicity diagnostic result in the embodiment of the present invention
The negative effect endangered) the pollution assignment that is classified.It is preferred that carry out " the Pyatyi the five-grade marking system " point system.Wherein,
According to the negative effect degree (poisonous effect) that biology is endangered compared with control group, soil ecology toxicity is diagnosed and tied
Fruit is categorized as：Serious pollution, serious pollution, intermediate pollution, slight pollution, potential pollution and pollution-free, wherein,
If poisonous effect is more than 80%, the levels of contamination for determining soil ecology toxicity diagnostic result is serious pollution, if
Put pollution and be entered as 5；
If poisonous effect is more than 50% and less than or equal to 80%, the pollution level of soil ecology toxicity diagnostic result is determined
Not Wei serious pollution, set pollution be entered as 4；
If poisonous effect is more than 30% and less than or equal to 50%, the pollution level of soil ecology toxicity diagnostic result is determined
Not Wei intermediate pollution, set pollution be entered as 3；
If poisonous effect is more than 15% and less than or equal to 30%, the pollution level of soil ecology toxicity diagnostic result is determined
Not Wei slight pollution, set pollution be entered as 2；
If poisonous effect is more than 5% and less than or equal to 15%, the pollution level of soil ecology toxicity diagnostic result is determined
Not Wei potential pollution, set pollution be entered as 1；
If poisonous effect is less than or equal to 5%, the levels of contamination of soil ecology toxicity diagnostic result is determined to be pollution-free,
Pollution is set to be entered as 0.
It should be noted that levels of contamination division and pollute assignment setting, can also basis be actually needed progress
Set, for example, according to being actually needed, levels of contamination can be arranged to 3 grades, it can also be provided that 8 grades etc..The embodiment of the present invention
The setting of division and pollution assignment to levels of contamination is not specifically limited.
Table 1 be poisonous effect with pollution evaluation mapping table, i.e., poisonous effect classification pollution assignment table, including poisonous effect with
Levels of contamination and the mapping relations for polluting assignment.
Table 1
In table 1, poisonous effect refers to the negative effect that biology is endangered, wherein, poisonous effect≤5% represents that biology exists
The effect that contaminated site is endangered is not notable, thus, set pollution to be entered as 0.
It should be noted that for same contaminated site, the soil ecology toxicity directly obtained by diagnostic test diagnoses
As a result have it is multiple, for example, different biological subjects, the specific experiment of the difference of different Testing index and use, can be obtained multiple
Soil ecology toxicity diagnostic result, each soil ecology toxicity diagnostic result are corresponding with the pollution assignment of poisonous effect mapping.
Step 102, according to the toxicity test classification chart pre-set, the kind of test belonging to diagnostic test, inquiry are obtained
The kind of test pre-set and experiment weight coefficient mapping table, what the kind of test belonging to the diagnostic test obtained mapped
Weight coefficient is tested, the toxicity test classification chart is classified based on toxic endpoints sensitiveness；
In this step, as it was previously stated, toxicity test (diagnostic test) includes：In short term/acute toxicity test and chronic/something lost
Toxicity test is passed, wherein, chronic/genetic toxicity test includes：For a long time/chronic toxicity test and genetic toxicity test.
In the embodiment of the present invention, based on the difference of toxic endpoints sensitiveness, each experiment that toxicity test is included is divided
Class, it is divided into a kind of experiment, the experiment of two classes and the experiment of three classes.
In the embodiment of the present invention, short-term/acute toxicity test is categorized as：One kind experiment and the experiment of two classes, it is chronic/to lose
Pass the corresponding three classes experiment of toxicity test.Wherein,
For toxic endpoints, one kind experiment includes：Lethal, germination percentage etc.；The experiment of two classes includes：Avoid, be luminous, enzyme activity suppression
System etc.；The experiment of three classes includes：Breeding, development, gene mutation, chromosome aberration etc.；
For Aquatic Toxicity Test, one kind experiment includes algal grown and suppresses experiment；The experiment of two classes includes：Photogen
Acute toxicity test and the acute motility inhibition test of Magna classes；That is, when being tested using one kind, Aquatic Toxicity Test
Carry out algal grown and suppress experiment, and using toxic endpoints of the lethal and germination percentage as experiment.
For terrestrial organism toxicity test, one kind experiment includes：Seed germination experiment, earthworm acute toxicity test；Two classes
Experiment includes：Terrestrial plant growth experiment, plant roots elongation test, plant physiology and biochemistry index determining, earthworm avlidance behavior examination
Test, earthworm Determination of Physiological And Biochemical Indices and microbial respiratory suppress；The experiment of three classes includes：Vegetable active experiment, earthworm breeding
Experiment, enchytrae reproduction test and snail young toxicity test；
For genetic toxicity test, the experiment of three classes includes：Genotoxicity, Ames test and earthworm
Earthworm Comet Assay.
In the embodiment of the present invention, for same tested species, generally, the sensitiveness for testing terminal is sequentially：It is numerous
Grow, develop and heredity test terminal ＞ behaviors, enzyme activity test terminal ＞ be lethal, germination percentage terminal, per a kind of experiment (one kind examination
Test, two classes experiment and three classes experiment) corresponding to toxotest terminal sensitivity range there is no specific numerical value.
In the embodiment of the present invention, divided according to the sensitiveness difference of the toxotest terminal of biological subject, for same
One biological subject, breeding, development and various genetoxic terminals are more sensitive, and testing experiment corresponding to such terminal is arranged into three
Class is tested；Behavior, luminous, enzyme activity suppress etc. test terminal typically than it is lethal, germination percentage is sensitive, behavior, luminous, enzyme activity are suppressed
The experiment of two classes is arranged to etc. testing experiment corresponding to non-lethal terminal；Testing experiment corresponding to germination percentage, lethal terminal is set
Tested for three classes.For example, using earthworm as biological subject, by taking the experiment of individual level as an example, earthworm acute toxicity test is one kind
Experiment, earthworm avlidance behavior experiment are tested for two classes, chronic toxicity and the something lost such as earthworm breeding, development experiment and earthworm Comet Assay
Toxicity test is passed to test for three classes.
Table 2 is the toxicity test classification chart of the embodiment of the present invention.In the embodiment of the present invention, one kind experiment and the experiment of two classes are all
It is short-term/acute toxicity test.
Table 2
In the embodiment of the present invention, because the toxic endpoints sensitivity differences of different biological subjects are larger and inhomogeneous
Obtained soil ecology toxicity diagnostic result is tested to differ greatly.Consider inhomogeneous experiment to soil ecology toxicity diagnostic result
Influence, experiment weight coefficient corresponding to different classes of experiment is set, to reflect the pollution level of contaminated site.
Table 3 is experiment weight coefficient list corresponding to the inhomogeneous experiment of the embodiment of the present invention, i.e. kind of test and experiment
Weight coefficient mapping table.
Table 3
It should be noted that the setting of experiment weight coefficient (effector) corresponding to kind of test, can also be according to reality
Border is needed to be arranged to other values, and the embodiment of the present invention is not specifically limited to this.
In the embodiment of the present invention, as alternative embodiment, when simultaneously same biological subject has carried out a kind of experiment and two classes
During experiment, if contradiction occurs in the soil ecology toxicity diagnostic result of two groups of experiments, knot is diagnosed with the soil ecology toxicity of weighting
Fruit (product of the soil ecology toxicity diagnostic result directly obtained and corresponding experiment weight coefficient) peak is defined.Citing comes
Say, to a certain contaminated site, a kind of experiment is respectively adopted in same biological subject and the experiment of two classes is examined soil ecology toxicity
It is disconnected, carry out soil ecology toxicity diagnosis, toxicity corresponding to obtained soil ecology toxicity diagnostic result if tested using two classes
Effect is ＞ 50%, then according to table 1,50% corresponding serious pollutions of poisonous effect ＞, 4 is entered as, according to table 3, two stage assay pair
The experiment weight coefficient (effector) answered is 0.8, then the soil ecology toxicity diagnostic result of two class experiment weighting is：4 with
0.8 is multiplied (3.2)；And using a kind of experiment to carry out soil ecology toxicity diagnosis, obtained poisonous effect is ＞ 30%, then basis
Table 1,30% corresponding intermediate pollutions of poisonous effect ＞, is entered as 3, according to table 3, experiment weight coefficient (effect corresponding to Primary Assay
Answer the factor) be 1, then the final soil ecology toxicity diagnostic result of one kind experiment is：3 are multiplied (3) with 1.Choose the soil of weighting
Earth eco-toxicity diagnostic result peak, obtained peak soil ecology toxicity diagnostic result are 3.2.
For another example using earthworm as biological subject, earthworm acute toxicity test (lethal effect) has been carried out respectively and earthworm returns
Behavior test is kept away, according to table 2, earthworm acute toxicity test belongs to a kind of experiment, and earthworm avlidance behavior experiment belongs to the experiment of two classes,
Assuming that the poisonous effect of earthworm acute experiment is ＞ 15%, then according to table 1 and table 3, the final soil life of the earthworm acute experiment
State toxicity diagnostic result is 2*1=2；Assuming that the poisonous effect of earthworm avlidance behavior experiment is ＞ 50%, then according to table 1 and table 3,
It is 4*0.8=3.2 that final soil ecology toxicity diagnostic result is tested in the earthworm avlidance behavior, chooses the soil ecology poison of weighting
Property diagnostic result peak, obtained peak soil ecology toxicity diagnostic result be 3.2.
As another alternative embodiment, in the embodiment of the present invention, enter when same poisonous effect terminal employs multiple species
Gone test when, using the test result of most sensitive species.
In the embodiment of the present invention, because the response range of each eco-toxicity diagnostic test is different, farthest to subtract
Few experimentation cost, complete to diagnose the eco-toxicity of soil with minimum experiment, level can also be set preferential toxicity test
Level, i.e., diagnosed, effectively to reduce test number (TN) by different level to the soil ecology toxicity of contaminated site.Specifically, it is aquatic
Biologic test level highest priority, short-term/acute toxicity test level priority are taken second place, long-term/chronic toxicity test level
Third, genetic toxicity test level priority is minimum for priority, and is sequentially tested according to level priority height.
Step 103, the Testing index that diagnostic test uses is determined, inquires about the Testing index pre-set and index weights
Mapping table, obtain the index weights for the Testing index mapping that the diagnostic test uses；
In this step, research shows, after pollutant emission is to environment, will be influenceed in different levels in ecological environment
Biology.Pollutant low dosage for a long time exposure or high dose exposure under, the influence to the ecosystem will be embodied in first molecule,
On the Testing index such as cell and histoorgan level, after certain level is arrived in accumulation, the high-level stage is progressively traveled to.From ecologic facies
For closing property and operability, the research of individual level is all in a more medium position, therefore, the ecology that various countries are recommended
Toxicity diagnostic criteria method, it is essentially all based on the toxicity test in bion level.
In the embodiment of the present invention, according to the height with ecological correlation, by the toxic endpoints (Testing index) of varying level
Different weighted values is assigned, is shown in Table 4.
Table 4
In table 4, the toxic endpoints (Testing index) of varying level, imparting has different index weightses.It is that is, right
Per the toxic endpoints of a kind of experiment in table 2, all comprising five kinds of Testing index in table 4, the corresponding index of every kind of Testing index
Weight.
It should be noted that step 101, step 102 and step 103 do not have point of sequencing, step 103 can be with
Perform, can also be performed before step 102 before step 101；Similarly, step 101 can be held after step 102
OK, can also be performed after step 103；Step 102 can perform before step 101, can also be held after step 103
OK.
Step 104, the pollution assignment of acquisition, experiment weight coefficient and index weights are applied to the soil pre-set
Eco-toxicity integrated integral formula, obtain soil ecology toxicity integrated integral；
In this step, each experiment is corresponding with a pollution assignment, an experiment weight coefficient and an index weights.Will pollution
All experiments that place is carried out are integrated, and obtain soil ecology toxicity integrated integral.
In the embodiment of the present invention, soil ecology toxicity integrated integral is calculated using weighted sum method.It is specific to calculate public affairs
Formula is as follows：
M=A+B+C
In formula,
M is soil ecology toxicity integrated integral；
A is the integrated integral of soil extract toxicity test；
n1For the biological subject kind number in soil extract toxicity test；
AiFor in soil extract toxicity test, pollution assignment corresponding to the poisonous effect of i-th kind of biological subject, i.e., i-th
Assignment is polluted corresponding to the soil ecology toxicity diagnostic result that kind biological subject is directly obtained by diagnostic test；
For in soil extract toxicity test, the experiment weight coefficient (effect of i-th kind of affiliated kind of test of biological subject
The factor)；
For in soil extract toxicity test, the index weights for the Testing index that i-th kind of biological subject uses；
B be terrestrial organism it is short-term/integrated integral of acute toxicity test；
n2For terrestrial organism it is short-term/acute toxicity test in biological subject kind number；
BjFor terrestrial organism it is short-term/acute toxicity test in, pollution assignment corresponding to the poisonous effect of jth kind biological subject；
For terrestrial organism it is short-term/acute toxicity test in, the experiment weight system of the affiliated kind of test of jth kind biological subject
Number (effector)；
For terrestrial organism it is short-term/acute toxicity test in, the index weights for the Testing index that jth kind biological subject uses；
C be terrestrial organism it is long-term/integrated integral of chronic toxicity test；
n3For terrestrial organism it is long-term/chronic toxicity test in biological subject kind number；
CkFor terrestrial organism it is long-term/chronic toxicity test in, pollution assignment corresponding to the poisonous effect of kth kind biological subject；
For terrestrial organism it is long-term/chronic toxicity test in, the experiment weight system of the affiliated kind of test of kth kind biological subject
Number (effector)；
For terrestrial organism it is long-term/chronic toxicity test in, the index weights for the Testing index that kth kind biological subject uses.
Step 105, soil ecology toxicity integrated integral and the ratio of individual level integration maximum are calculated, and according to calculating
Ratio determine the soil ecology Severity level of contaminated site.
In this step, maximum (M is integrated according to soil ecology toxicity integrated integral (M) and individual levelmax) ratio
(R) soil ecology Severity level is determined.I.e.：
In formula,
R is tested soil ecology harmfulness score value；
MmaxMaximum is integrated for individual level, the soil ecology toxicity integrated integral respectively tested when 0.6 is taken for index weights
Maximum.
In the embodiment of the present invention, individual level integration maximum value calculation formula is as follows：
In formula,
Ai maxFor in soil extract toxicity test, greatest contamination assignment corresponding to the poisonous effect of i-th kind of biological subject；
In the embodiment of the present invention, pollution assignment value is 5；
For in soil extract toxicity test, the ceiling effect factor of i-th kind of biological subject；Value is 1.
Bj maxFor terrestrial organism it is short-term/acute toxicity test in, it is maximum dirty corresponding to the poisonous effect of jth kind biological subject
Assignment is contaminated, pollution value is 5；
For terrestrial organism it is short-term/acute toxicity test in, the ceiling effect factor of jth kind biological subject, value 1；
Ck maxFor terrestrial organism it is long-term/chronic toxicity test in, it is maximum corresponding to the poisonous effect of the 7th kind of biological subject
Pollute assignment, value 5；
For terrestrial organism it is long-term/chronic toxicity test in, the ceiling effect factor of kth kind biological subject, value 1；
0.6 is the index weights tested when Testing index is individual level.
In the embodiment of the present invention, soil ecology Severity level is divided into 4 grades, is respectively：It is high to endanger level, high harm
Level, it is middle endanger level and less harmful level, wherein,
R >=0.8, endanger level to be high；
0.5≤R ＜ 0.8, endanger level for height；
0.3≤R ＜ 0.5, endanger level in being；
R ＜ 0.3, it is less harmful level.
From above-mentioned, the method for the optimization contaminated site soil ecology toxicity diagnostic result of the embodiment of the present invention, pass through
Consider each influence factor in contaminated site diagnostic test, respectively poisonous effect corresponding to soil ecology toxicity diagnostic result is set
Pollution assignment is put, the kind of test belonging to diagnostic test sets corresponding experiment weight coefficient, and sets phase for each Testing index
The index weights answered, based on pollution assignment, experiment weight coefficient corresponding to each soil ecology toxicity diagnostic result tested and obtained
And index weights calculate soil ecology toxicity integrated integral, according to individual level integration maximum, to the soil being calculated
Eco-toxicity integrated integral carries out soil ecology Severity level assessment so that the accuracy of assessment is high, is not easy by indivedual tested
The influence of biology and the Testing index chosen, the actual pollution situation of contaminated site can be reflected.
Fig. 2 is the apparatus structure schematic diagram that the embodiment of the present invention optimizes contaminated site soil ecology toxicity diagnostic result.Ginseng
See Fig. 2, the device includes：Pollute assignment acquisition module, experiment weight coefficient acquisition module, index weights acquisition module, soil
Eco-toxicity integrated integral computing module and Severity level evaluation module, wherein,
Assignment acquisition module is polluted, for gathering the soil ecology toxicity diagnostic result directly obtained by diagnostic test,
The poisonous effect and pollution evaluation mapping table pre-set is inquired about, toxicity corresponding to the soil ecology toxicity diagnostic result is obtained and imitates
The pollution assignment that should be mapped；
In the embodiment of the present invention, poisonous effect includes with pollution evaluation mapping table：
If poisonous effect is more than 80%, the levels of contamination for determining soil ecology toxicity diagnostic result is serious pollution, if
Put pollution and be entered as 5；
If poisonous effect is more than 50% and less than or equal to 80%, the pollution level of soil ecology toxicity diagnostic result is determined
Not Wei serious pollution, set pollution be entered as 4；
If poisonous effect is more than 30% and less than or equal to 50%, the pollution level of soil ecology toxicity diagnostic result is determined
Not Wei intermediate pollution, set pollution be entered as 3；
If poisonous effect is more than 15% and less than or equal to 30%, the pollution level of soil ecology toxicity diagnostic result is determined
Not Wei slight pollution, set pollution be entered as 2；
If poisonous effect is more than 5% and less than or equal to 15%, the pollution level of soil ecology toxicity diagnostic result is determined
Not Wei potential pollution, set pollution be entered as 1；
If poisonous effect is less than or equal to 5%, the levels of contamination of soil ecology toxicity diagnostic result is determined to be pollution-free,
Pollution is set to be entered as 0.
Weight coefficient acquisition module is tested, for according to the toxicity test classification chart pre-set, obtaining diagnostic test institute
The kind of test of category, the kind of test pre-set is inquired about with experiment weight coefficient mapping table, the diagnostic test institute obtained
The experiment weight coefficient of the kind of test mapping of category, the toxicity test classification chart are classified based on toxic endpoints sensitiveness；
In the embodiment of the present invention, toxicity test classification chart includes：
Short-term/acute toxicity test is categorized as：One kind experiment and the experiment of two classes, chronic/genetic toxicity test are corresponding
Three classes are tested, wherein,
For toxic endpoints, one kind experiment includes：Lethal, germination percentage etc.；The experiment of two classes includes：Avoid, be luminous, enzyme activity suppression
System etc.；The experiment of three classes includes：Breeding, development, gene mutation, chromosome aberration etc.；
For Aquatic Toxicity Test, one kind experiment includes algal grown and suppresses experiment；The experiment of two classes includes：Photogen
Acute toxicity test and the acute motility inhibition test of Magna classes；
For terrestrial organism toxicity test, one kind experiment includes：Seed germination experiment, earthworm acute toxicity test；Two classes
Experiment includes：Terrestrial plant growth experiment, plant roots elongation test, plant physiology and biochemistry index determining, earthworm avlidance behavior examination
Test, earthworm Determination of Physiological And Biochemical Indices and microbial respiratory suppress；The experiment of three classes includes：Vegetable active experiment, earthworm breeding
Experiment, enchytrae reproduction test and snail young toxicity test；
For genetic toxicity test, the experiment of three classes includes：Genotoxicity, Ames test and earthworm
Earthworm Comet Assay.
Kind of test includes with experiment weight coefficient mapping table：
The experiment weight coefficient of one kind experiment mapping is 1；
The experiment weight coefficient of two classes experiment mapping is 0.8；
The experiment weight coefficient of three classes experiment mapping is 0.5.
Index weights acquisition module, the Testing index used for determining diagnostic test, inquires about the detection pre-set and refers to
The mapping table of mark and index weights, obtain the index weights for the Testing index mapping that the diagnostic test uses；
In the embodiment of the present invention, the mapping table of Testing index and index weights includes：
Testing index is molecule/cellular level, and the index weights of mapping are 0.2；
Testing index is that tissue/organ is horizontal, and the index weights of mapping are 0.3；
Testing index is individual level, and the index weights of mapping are 0.6；
Testing index is population/community level, and the index weights of mapping are 0.8；
Testing index is the ecosystem/landscape level, and the index weights of mapping are 1.
Soil ecology toxicity integrated integral computing module, for the pollution assignment of acquisition, experiment and to be referred to weight coefficient
Mark weight is applied to the soil ecology toxicity integrated integral formula pre-set, obtains soil ecology toxicity integrated integral；
In the embodiment of the present invention, soil ecology toxicity integrated integral is calculated using weighted sum method.It is specific to calculate public affairs
Formula is as follows：
M=A+B+C
Severity level evaluation module, for calculating soil ecology toxicity integrated integral and individual level integration maximum
Ratio, and determine according to the ratio of calculating the soil ecology Severity level of contaminated site.
In the embodiment of the present invention, maximum (M is integrated according to soil ecology toxicity integrated integral (M) and individual levelmax)
Ratio (R) determines soil ecology Severity level.I.e.：
Individual level integration maximum value calculation formula is as follows：
In the embodiment of the present invention, soil ecology Severity level is divided into 4 grades, is respectively：It is high to endanger level, high harm
Level, it is middle endanger level and less harmful level, wherein,
R >=0.8, endanger level to be high；
0.5≤R ＜ 0.8, endanger level for height；
0.3≤R ＜ 0.5, endanger level in being；
R ＜ 0.3, it is less harmful level.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvement for being made etc., the guarantor of the present invention should be included in
Within the scope of shield.
Claims (7)
1. a kind of method for optimizing contaminated site soil ecology toxicity diagnostic result, this method include：
The soil ecology toxicity diagnostic result directly obtained by diagnostic test is gathered, inquires about the poisonous effect pre-set and dirt
Evaluation mapping table is contaminated, obtains the pollution assignment that poisonous effect corresponding to the soil ecology toxicity diagnostic result maps；
According to the toxicity test classification chart pre-set, the kind of test belonging to diagnostic test is obtained, inquires about the examination pre-set
Classification and experiment weight coefficient mapping table are tested, the experiment weight system of the kind of test mapping belonging to the diagnostic test obtained
Number；
The Testing index that diagnostic test uses is determined, inquires about the mapping table of the Testing index pre-set and index weights,
Obtain the index weights for the Testing index mapping that the diagnostic test uses；
The soil ecology toxicity that the pollution assignment of acquisition, experiment weight coefficient and index weights are applied to pre-set is integrated
Integral formula, obtain soil ecology toxicity integrated integral；
Soil ecology toxicity integrated integral and the ratio of individual level integration maximum are calculated, and dirt is determined according to the ratio of calculating
Contaminate the soil ecology Severity level in place；
The soil ecology toxicity integrated integral is calculated using following formula：
M=A+B+C
In formula,
M is soil ecology toxicity integrated integral；
A is the integrated integral of soil extract toxicity test；
B be terrestrial organism it is short-term/integrated integral of acute toxicity test；
C be terrestrial organism it is long-term/integrated integral of chronic toxicity test；
Wherein,
In formula,
n1For the biological subject kind number in soil extract toxicity test；
AiFor in soil extract toxicity test, assignment is polluted corresponding to the poisonous effect of i-th kind of biological subject；
For in soil extract toxicity test, the experiment weight coefficient of i-th kind of affiliated kind of test of biological subject；
For in soil extract toxicity test, the index weights for the Testing index that i-th kind of biological subject uses；
n2For terrestrial organism it is short-term/acute toxicity test in biological subject kind number；
BjFor terrestrial organism it is short-term/acute toxicity test in, pollution assignment corresponding to the poisonous effect of jth kind biological subject；
For terrestrial organism it is short-term/acute toxicity test in, the experiment weight coefficient of the affiliated kind of test of jth kind biological subject；
For terrestrial organism it is short-term/acute toxicity test in, the index weights for the Testing index that jth kind biological subject uses；
n3For terrestrial organism it is long-term/chronic toxicity test in biological subject kind number；
CkFor terrestrial organism it is long-term/chronic toxicity test in, pollution assignment corresponding to the poisonous effect of kth kind biological subject；
For terrestrial organism it is long-term/chronic toxicity test in, the experiment weight coefficient of the affiliated kind of test of kth kind biological subject；
For terrestrial organism it is long-term/chronic toxicity test in, the index weights for the Testing index that kth kind biological subject uses；
The individual level integration maximum is calculated using following formula：
In formula,
AimaxFor in soil extract toxicity test, greatest contamination assignment corresponding to the poisonous effect of i-th kind of biological subject；
For in soil extract toxicity test, the ceiling effect factor of i-th kind of biological subject；
BjmaxFor terrestrial organism it is short-term/acute toxicity test in, greatest contamination assignment corresponding to the poisonous effect of jth kind biological subject；
For terrestrial organism it is short-term/acute toxicity test in, the ceiling effect factor of jth kind biological subject；
CkmaxFor terrestrial organism it is long-term/chronic toxicity test in, greatest contamination assignment corresponding to the poisonous effect of kth kind biological subject；
For terrestrial organism it is long-term/chronic toxicity test in, the ceiling effect factor of kth kind biological subject；
0.6 is the index weights tested when Testing index is individual level.
2. the method as described in claim 1, the poisonous effect includes with pollution evaluation mapping table：
If poisonous effect is more than 80%, the levels of contamination for determining soil ecology toxicity diagnostic result is serious pollution, is set dirty
Dye is entered as 5；
If poisonous effect is more than 50% and less than or equal to 80%, the levels of contamination for determining soil ecology toxicity diagnostic result is
Serious pollution, pollution is set to be entered as 4；
If poisonous effect is more than 30% and less than or equal to 50%, the levels of contamination for determining soil ecology toxicity diagnostic result is
Intermediate pollution, pollution is set to be entered as 3；
If poisonous effect is more than 15% and less than or equal to 30%, the levels of contamination for determining soil ecology toxicity diagnostic result is
Slight pollution, pollution is set to be entered as 2；
If poisonous effect is more than 5% and less than or equal to 15%, the levels of contamination for determining soil ecology toxicity diagnostic result is
Potential pollution, pollution is set to be entered as 1；
If poisonous effect is less than or equal to 5%, the levels of contamination for determining soil ecology toxicity diagnostic result is pollution-free, is set
Pollution is entered as 0.
3. the method as described in claim 1, the diagnostic test includes：In short term/acute toxicity test and chronic/heredity poison
Property experiment, wherein, chronic/genetic toxicity test includes：For a long time/chronic toxicity test and genetic toxicity test.
4. method as claimed in claim 3, the short-term/acute toxicity test is categorized as：One kind experiment and the experiment of two classes,
The corresponding three classes experiment of chronic/genetic toxicity test, wherein,
For toxic endpoints, one kind experiment includes：Lethal, germination percentage；The experiment of two classes includes：Avoid, luminous, enzyme activity suppresses；Three
Class experiment includes：Breeding, development, gene mutation, chromosome aberration；
For Aquatic Toxicity Test, one kind experiment includes algal grown and suppresses experiment；The experiment of two classes includes：Photogen is acute
Toxicity test and the acute motility inhibition test of Magna classes；
For terrestrial organism toxicity test, one kind experiment includes：Seed germination experiment, earthworm acute toxicity test；Two classes are tested
Including：Terrestrial plant growth experiment, plant roots elongation test, plant physiology and biochemistry index determining, earthworm avlidance behavior experiment, earthworm
Earthworm Determination of Physiological And Biochemical Indices and microbial respiratory suppress；The experiment of three classes includes：Vegetable active experiment, earthworm reproduction test,
Enchytrae reproduction test and snail young toxicity test；
For genetic toxicity test, the experiment of three classes includes：Genotoxicity, Ames test and earthworm broom
Satellite experiment.
5. method as claimed in claim 4, the kind of test includes with experiment weight coefficient mapping table：
The experiment weight coefficient of one kind experiment mapping is 1；
The experiment weight coefficient of two classes experiment mapping is 0.8；
The experiment weight coefficient of three classes experiment mapping is 0.5.
6. the method as described in claim 1, the determination soil ecology Severity level is determined using following formula：
In formula,
R is tested soil ecology harmfulness score value；
If R >=0.8, level is endangered to be high；
If 0.5≤R ＜ 0.8, level is endangered for height；
If 0.3≤R ＜ 0.5, level is endangered in being；
It is less harmful level if R ＜ 0.3.
7. a kind of device for optimizing contaminated site soil ecology toxicity diagnostic result, the device include：
Assignment acquisition module is polluted, for gathering the soil ecology toxicity diagnostic result directly obtained by diagnostic test, inquiry
The poisonous effect that pre-sets and pollution evaluation mapping table, obtain the soil ecology toxicity diagnostic result corresponding to poisonous effect reflect
The pollution assignment penetrated；
Weight coefficient acquisition module is tested, for according to the toxicity test classification chart pre-set, obtaining belonging to diagnostic test
Kind of test, the kind of test pre-set is inquired about with experiment weight coefficient mapping table, belonging to the diagnostic test obtained
The experiment weight coefficient of kind of test mapping, the toxicity test classification chart are classified based on toxic endpoints sensitiveness；
Index weights acquisition module, the Testing index used for determining diagnostic test, inquire about the Testing index that pre-sets with
The mapping table of index weights, obtain the index weights for the Testing index mapping that the diagnostic test uses；
Soil ecology toxicity integrated integral computing module, for the pollution assignment of acquisition, experiment weight coefficient and index to be weighed
It is applied to the soil ecology toxicity integrated integral formula pre-set again, obtains soil ecology toxicity integrated integral；
Severity level evaluation module, for calculating the ratio of soil ecology toxicity integrated integral and individual level integration maximum
It is worth, and the soil ecology Severity level of contaminated site is determined according to the ratio of calculating；
The soil ecology toxicity integrated integral is calculated using following formula：
M=A+B+C
In formula,
M is soil ecology toxicity integrated integral；
A is the integrated integral of soil extract toxicity test；
B be terrestrial organism it is short-term/integrated integral of acute toxicity test；
C be terrestrial organism it is long-term/integrated integral of chronic toxicity test；
Wherein,
In formula,
n1For the biological subject kind number in soil extract toxicity test；
AiFor in soil extract toxicity test, assignment is polluted corresponding to the poisonous effect of i-th kind of biological subject；
For in soil extract toxicity test, the experiment weight coefficient of i-th kind of affiliated kind of test of biological subject；
For in soil extract toxicity test, the index weights for the Testing index that i-th kind of biological subject uses；
n2For terrestrial organism it is short-term/acute toxicity test in biological subject kind number；
BjFor terrestrial organism it is short-term/acute toxicity test in, pollution assignment corresponding to the poisonous effect of jth kind biological subject；
For terrestrial organism it is short-term/acute toxicity test in, the experiment weight coefficient of the affiliated kind of test of jth kind biological subject；
For terrestrial organism it is short-term/acute toxicity test in, the index weights for the Testing index that jth kind biological subject uses；
n3For terrestrial organism it is long-term/chronic toxicity test in biological subject kind number；
CkFor terrestrial organism it is long-term/chronic toxicity test in, pollution assignment corresponding to the poisonous effect of kth kind biological subject；
For terrestrial organism it is long-term/chronic toxicity test in, the experiment weight coefficient of the affiliated kind of test of kth kind biological subject；
For terrestrial organism it is long-term/chronic toxicity test in, the index weights for the Testing index that kth kind biological subject uses；
The individual level integration maximum is calculated using following formula：
In formula,
AimaxFor in soil extract toxicity test, greatest contamination assignment corresponding to the poisonous effect of i-th kind of biological subject；
For in soil extract toxicity test, the ceiling effect factor of i-th kind of biological subject；
BjmaxFor terrestrial organism it is short-term/acute toxicity test in, greatest contamination corresponding to the poisonous effect of jth kind biological subject is assigned
Value；
For terrestrial organism it is short-term/acute toxicity test in, the ceiling effect factor of jth kind biological subject；
CkmaxFor terrestrial organism it is long-term/chronic toxicity test in, greatest contamination corresponding to the poisonous effect of kth kind biological subject is assigned
Value；
For terrestrial organism it is long-term/chronic toxicity test in, the ceiling effect factor of kth kind biological subject；
0.6 is the index weights tested when Testing index is individual level.
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