CN105294883A - Method for preparing beet pectin in diluted acid-pressing way - Google Patents
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Abstract
The invention discloses a method for preparing beet pectin in a diluted acid-pressing way. The method comprises the following steps: slowly refrigerating fresh pressed pulp or stored refrigerated pressed pulp by taking wet pulp having the water content being 75 to 80 percent as a raw material, washing with clear water, draining off, loading into an extraction tank, and adding deionized water; adjusting the pH value of a solution with acid; soaking at 65-85 DEG C; periodically raising the pressure in the extraction tank in a soaking process, keeping the pressure, recovering to normal pressure, raising the pressure and lowering the pressure to the normal pressure, wherein a pressure changing period lasts for 1 to 3 times; stirring the materials in the extracting tank to obtain crude filtrate; adding a carbonate solution into the crude filtrate, and finely filtering in a filter press; concentrating the finely-filtered pectin extracting solution, and precipitating; removing a solvent, and drying to obtain the beet pectin. The method is mild in reaction conditions and simplified in process, the problem of poor mass transfer caused by absorption expansion of beet wet meal is solved, degradation of pectin molecules and the loss of neutral sugars on side chains are avoided, the yield and quality of the pectin are improved, and the processing cost is lowered.
Description
Technical field
The present invention relates to a kind of pectin technology of preparing, particularly the auxiliary diluted acid of pressurization directly extracts the method for pectin from beet wets the dregs of rice, belongs to field of deep processing of farm products.
Background technology
Beet is as the sugar crop cultivation history of existing 200 years so far, and world today's beet cultivated area accounts for 48% of sugar crop, occupies the 2nd inferior to sugarcane.Beet pulp is the by product produced after sugar is carried in beet root chopping hot dipping, the wet dregs of rice are called the pressure dregs of rice after extruding removing portion of water, its moisture content is about 75 ~ 80%, and pressure dregs of rice output accounts for 20 ~ 30% of beet dry-matter, the beet sugar manufacture by product that the amount of being is concentrated greatly.Sugar refinery is all produce at north of china in winter, and the pressure dregs of rice are easy to naturally frozen storage.The pressure dregs of rice of current domestic and international each beet-sugar factory are mainly squeezed into granular dregs, used as fodder through 600 ~ 800 DEG C of dryings, do not carry out higher value application.
Beet pectin is a kind of mixed polysaccharide that extensively there is beet cell wall and cell middle layer, and the sampling analysis according to China different areas confirms, in beet pulp containing 15 ?28% pectin.The structure of beet pectin and the processing mode of raw material, Isolation and purification technology are relevant; research shows the neutral sugar of beet pulp pectin, degree of acetylation and protein content all higher than the pectin in orange peel and apple residue source; have good emulsification, stable and thickening properties; compared with conventional gum arabic; the emulsifying activity of beet pectin advantageously, is therefore with a wide range of applications in food and medicine industry.
Worldwide beet pectin does not also form suitability for industrialized production at present, and particularly China still can not meet the need of market with the pectin output that oranges and tangerines and Fructus Mali pumilae dregs and peel are raw material.If in conjunction with the resourceful advantage of China's beet pulp, can develop beet pectin product of good performance, can not only fill up the blank in market, cheap beet pulp can also be made to appreciate, and the recycling that can be beet sugar manufacturing industry brings considerable economic benefit.
According to the kind of Extraction solvent, pectin extraction method can be divided into acid system, alkaline process and chelate method.Acid system has high, residual less, the easy and simple to handle advantage of efficiency, is widely adopted.Usually, (insoluble) protopectin-in cell walls for Extraction solvent, is converted into soluble pectin by hot acid effect with organic acid or mineral acid by acid system.This process generally in pH1.3 ~ 3.0,60 ~ 90 DEG C, carry out (YapoB.M.Pectinquantity under 30 ~ 360min, compositionandphysicochemicalbehaviourasinfluencedbythep urificationprocess [J] .FoodResearchInternational.2009 (8): 1197 – 1202.), be thus also referred to as hot acid method.The subject matter that hot acid method exists is easy depolymerized pectin molecular structure, especially structure (particularly the arabinose residues) (YapoB.M. that the acid such as methoxyl group, tral sugar side chains is unstable, RobertC., EtienneI., etal.Effectofextractionconditionsontheyield, purityandsurfacepropertiesofsugarbeetpulppectinextracts [J] .FoodChemistry.2007,100 (4): 1356 – 1364; Thibault, J. ?F., Renard, C.M.G.C., Axelos, M.A.V., Roger, P., & Cre ' peau, M. ?J. (1993) .Studiesofthelengthofhomogalacturonicregionsinpectinsbya cidhydrolysis.CarbohydrateResearch, 238,271 – 286).In order to overcome this problem, Chinese scholars develops the chelate approach of milder, but because sequestrant easily remains, therefore limit this method further application (LevigneS. industrially, RaletM., ThibaultJ.F.Characterisationofpectinsextractedfromfreshs ugarbeetunderdifferentconditionsusinganexperimentaldesig n [J] .CarbohydratePolymers.2002,49 (2): 145 – 153).
Beet pectin extractive technique has been reported.201410326603.0) and a kind of method of microwave radiation exaraction beet pectin the people such as Zhang Haiyan disclose a kind of method (number of patent application: of salt method beet pectin; The safe China of wood waits people to disclose a kind of method (number of patent application: 201310247157.X) of physics assisted extraction beet pectin; The people such as Wang Yong disclose a kind of beet pectin and extracting method (number of patent application: 201310700191.8) thereof.But existing beet pectin extraction scheme be all with the dry dregs of rice for extract raw material basis on set up, and the enforcement of the program needs to carry out the pre-treatment such as drying, pulverizing to the beet dregs of rice that wet.Not only technique is loaded down with trivial details for this method, power consumption, also increases the loss of raw material to a certain extent.Secondly, in leaching process, the beet undergoes rapid expansion after dregs of rice water suction that wets increases the viscosity of system, and this have impact on beet pectin mass transfer effect.Although extend the detrimentally affect that extraction time can compensate the generation of mass transfer difference, the prolongation of heat-up time exacerbates again the palliating degradation degree of pectin molecule, and finally causes the quality of pectin to decline.Therefore, keep extraction yield in advance under, developing a kind of contributing to keeps the method for beet pectin structural integrity to have important practical significance.
Summary of the invention
For the shortcoming and defect of existing pectin extraction technology, the present invention wets from beet the physicochemical characteristic of the dregs of rice, aims to provide the pectin preparation method that a kind of reaction conditions is gentle, flow process is simple, extraction efficiency is high.
The leaching process of pectin is that protopectin-constantly dissolves, and to the outside process easily spread of cell walls.From the angle of mass transfer, this process can be subdivided into again: (1) pectin is surface from plant tissue internal diffusion to stationary phase; (2) pectin on stationary phase surface is diffused into (MinkovS. in solution again by concentration gradient, MinchevA., PaevK.Modellingofthehydrolysisandextractionofapplepectin [J] .JournalofFoodEngineering.1996,29 (1): 107 – 113).When the factors such as temperature, solid-liquid ratio, stirring velocity are identical, the rate of mass transfer of pectin is also closely related with the physicochemical characteristic of raw material.When the coefficient of expansion of raw material is large, viscosity height time, the speed that pectin yearns for diffusion from plant tissue is slower.Therefore, select suitable extractive technique according to the physicochemical characteristic of raw material, for the extraction efficiency improving pectin, there is important effect.The present invention is for raw material with sugar refinery by product pressure dregs of rice, feature is that raw material need not dry and rehydration swelling process, the mode of pressurizeing instantaneously is coordinated in dilute acid soln to open cell passage, contributes to the problem overcoming thickness slag liquid mass transfer difference, make pectin be easy to stripping and be diffused in solution.
Object of the present invention is achieved through the following technical solutions:
Xi Suan ?pressurization prepare the method for beet pectin, comprise the following steps:
Step 1: by percentage to the quality, the wet dregs of rice with moisture 75 ~ 80% are raw material, freeze slow for the freezing pressure dregs of rice of the fresh pressure dregs of rice or storage, clear water washing, drain rear loading extractor, add deionized water; Controlling the pressure dregs of rice with deionized water quality than being 1:10 ?20, is 2.0 ?3.4 by the pH value of acid-conditioning solution; The acid added is hydrochloric acid, sulfuric acid, nitric acid or citric acid, soaks at 65 ~ 85 DEG C;
Step 2: boost to 0.1 ~ 1.0Mpa to the extractor cycle in immersion process, keeps pressure 1 ~ 5min, after being returned to normal pressure 20 ~ 60min, then to boost and decompression is returned to normal pressure; The pressure alteration cycle is 1 ~ 3 time, and total soak time is 100 ~ 180min, comprises the pressure alteration cycle;
Step 3: stir material in extractor, controls stirring velocity 60 ~ 200rpm, obtains coarse filtration liquid;
Step 4: add carbonate solution in coarse filtration liquid, makes the suspended substance in precipitation and absorption coarse filtration liquid and calcium and magnesium salt ash content, and extracting solution, after mechanical deslagging device precipitation separation and suspended substance, enters pressure filter and carries out essence filtration; The carbonate added is CaCO
3or Na
2cO
3, add-on is make the pH value of coarse filtration liquid be 2.5 ~ 4.5;
Step 5: the pectin extracting soln after essence being filtered concentrates, gradually in the pectin solution after concentrated adds ethanol, and pectin is dewatered precipitating; Alcohol analyse after through extruding or centrifugal desolvation, drying obtains beet pectin.
For realizing the object of the invention further, preferably, described pressure filter is butterfly or plate and frame(type)filter press.
Preferably, the described gradual alcoholic solvent that adds is that the different liquid level point analysing tank at alcohol instill ethanolic soln simultaneously, and the quality adding ethanol is 3 ~ 5 times of pectin solution.
Preferably, described ethanol mass concentration is 75 ~ 95%.
Preferably, described concentrated be that at 40 ~ 60 DEG C of temperature, evaporate removing moisture be 1 ~ 5% to containing solid quality percentage composition for pectin extracting soln after essence being filtered; Concentrated vaporizer is that single-action or multiple-effect series combination form.
Preferably, described drying refer to by the solid wet pectin of centrifugal exsolution agent at <60 DEG C of temperature dry obtain water content lower than 10% pectin.
Compared with prior art, the present invention has the following advantages:
1, the direct garden beet of the inventive method dregs of rice that wet are raw material, need not pre-treatment and reconstitution process when extracting such as drying, pulverizing, and beet cell adds cell passage through overheatedly to rise, after freeze thawing, makes pectin be easy to from organizing internal diffusion to solid phase surface and solution; Easily, extraction yield is high, and flow process is short in pectin molecule diffusion;
2, few by acid amount in the inventive method process, its method is little to pectin molecule structural damage, and the purity of product is high, mild condition, not etching apparatus, free from environmental pollution;
3, adopt gradual alcohol during alcohol precipitation of the present invention, avoid local too high containing alcohol, pectin precipitation parcel impurity, forms indissoluble particle;
4, compared with traditional technology, this technology reaction conditions is gentle, process simplification, overcomes the beet dregs of rice that wet and absorbs the mass transfer difference problem expanding and cause, and also helps avoid the loss of neutral sugar on the degraded of pectin molecule and side chain, improve yield and the quality of pectin, cut down finished cost simultaneously; Technological line is simple, can realize scale operation.
Accompanying drawing explanation
Fig. 1 is the typical curve of the galacturonic acid content measuring products obtained therefrom in the embodiment of the present invention;
Fig. 2 is the molecular weight distribution curve that the embodiment of the present invention and art methods extract pectin sample.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described, but the scope of protection of present invention is not limited to the scope of embodiment statement.
Embodiment 1
Take the fresh beet pressure dregs of rice 1200g of moisture 80% (mass content), residual sugar is removed with 25 DEG C of water hydro-peenings, drain rear loading extractor, add deionized water, the mass ratio of the beet pressure dregs of rice and deionized water is 1:18, and add the hydrochloric acid that concentration is 2mol/L, adjust ph is 3.0, after 50rpm stirs, at 82 DEG C, constant temperature leaves standstill 30min, and extractor boosts to 0.1Mpa, holds pressure 4min, tensimeter is returned to 0.0Mpa, 80rpm stirs 30min and leaves standstill, then boosts to 0.13Mpa, holds pressure 3min, reduce to normal pressure, 120rpm stirs 30min.Bent sieved filter solid-liquid separation, adds the NaCO that mass concentration is 5% in filtrate
3solution, maintaining pH value is 4, and filter-cloth filtering, filtrate is concentrated into 30% of original volume with vaporizer at 60 DEG C.Filtrate moves in Alcohol-settling tank, and with the ethanol of the progressive dropping mass concentration 85% of two-point, add-on is to filtrate 3.2 times of volumes.Less than 10 DEG C leave standstill 12 hours, be separated 30min with the whizzer of 10000rpm, then be respectively the washing with alcohol 2 times of 85%, 90% by mass concentration successively, wash away soluble sugar and alcohol dissolubility impurity, centrifugation, at 55 DEG C, vacuum-drying obtains light gray powder target product.Collect above-mentioned ethanolic soln at different levels in order to reuse.
Embodiment 2
Cheng Qu ?the beets pressure dregs of rice 2000g of 15 DEG C of storages, the slow rear use 35 DEG C of warm water hydro-peenings that freeze remove the impurity such as pressure dregs of rice surface residual sugar, drain rear moisture 75%, load pot for solvent extraction, add deionized water, the mass ratio of the beet pressure dregs of rice and deionized water is 1:20, add the nitric acid that concentration is 1.5mol/L, adjust ph is 2.5, after 45rpm stirs, at 78 DEG C, constant temperature leaves standstill 35min, 0.12Mpa is boosted in tank, hold pressure 3min, tensimeter is returned to 0.0Mpa, 90rpm stirs 20min and leaves standstill, boost to 0.15Mpa again, hold pressure 4min, reduce to normal pressure, 100rpm stirs 30min.Solid-liquid separation is carried out in filtration, adds the NaCO that mass concentration is 4% in filtrate
3solution, maintaining pH value is 3.3, and vacuum filtration, filtrate is concentrated into 25% of original volume at 57 DEG C.Filtrate moves in Alcohol-settling tank, and be the ethanol of 87% with the progressive dropping mass concentration of bikini, add-on is to filtrate 3.0 times of volumes.Less than 15 DEG C leave standstill 13 hours, are separated 20min with the whizzer of 12000rpm, then are respectively the washing with alcohol 3 times of 83%, 85%, 90% by mass concentration successively, and wash away alcohol dissolubility impurity, centrifugation, at 60 DEG C, vacuum-drying obtains beet pectin.Collect above-mentioned ethanolic soln at different levels in order to reuse.
Embodiment 3
Cheng Qu ?the beet pressure dregs of rice 1800g of storage at 5 DEG C, the slow rear use 30 DEG C of water hydro-peenings that freeze remove residual sugar, drain rear moisture 78% (mass content), load in extractor, add deionized water, the mass ratio of the beet pressure dregs of rice and deionized water is 1:15, add the phosphoric acid that concentration is 1.2mol/L, adjust ph is 3.2, after 50rpm stirs, at 80 DEG C, constant temperature leaves standstill 50min, pot for solvent extraction boosts to 0.15Mpa, hold pressure 5min, tensimeter is returned to 0.0Mpa, stir 20min to leave standstill, boost to 0.20Mpa again, hold pressure 3min, reduce to normal pressure, 120rpm stirs 30min.Extracting solution obtains clear filtrate through Plate Filtration, then in filtrate, add the NaCO that mass concentration is 3%
3solution, maintaining pH value is 3.2, and filter-cloth filtering, filtrate is concentrated into 23% of original volume with vaporizer at 55 DEG C.Filtrate moves in Alcohol-settling tank, and take the progressive dropping mass concentration of bikini as the ethanol of 83% concentration, add-on is to filtrate 3.5 times of volumes.Less than 8 DEG C leave standstill 10 hours, are separated 30min with the whizzer of 8000rpm, then are respectively the washing with alcohol 2 times of 82%, 89% by mass concentration successively, and wash away soluble sugar and alcohol dissolubility impurity, centrifugation, at 56 DEG C, vacuum-drying obtains beet pectin.Collect above-mentioned ethanolic soln at different levels in order to reuse.
(traditional hot acid method's technique gathers traditional hot acid method's technique of comparing embodiment (experiment sample) gained pectin sample and improvement, pH value is 1.3 ~ 3.0, temperature is 60 ~ 90 DEG C, the time is 30 ~ 360min, not pressurization, do not relate to intermittent pressurized, raw material is siccative) the obtained difference of pectin sample (control sample) in extraction yield and chemical composition, pectin molecule amount, molecular structure etc.Embodiment sample is from embodiment 2; Do not carry out pressure treatment in control sample preparation process, but wet the dregs of rice for extracting raw material with beet, solid-liquid ratio is 1:20, extract that pH value is 2.5, extraction time is 100min; The method that the detection of experiment sample and control sample specifies according to GB/T25533-2010 and additive method (see table 1) detect, and its result is as follows:
The Testing index of table 1 gained sample
| Project | Experiment sample | Control sample | National regulations | The method of inspection |
| Extraction yield, (w/%) | 11.7 | 10.3 | / | / |
| Loss on drying, (w/%) | 11.0 | 11.0 | ≦12.0 | GB 5009.3 |
| Sulfurous gas, (mg/kg) | Do not detect | Do not detect | ≦5.0 | GB/T 5009.34 |
| Acid insoluble ash, (w/%) | <0.1 | <0.1 | ≦1.0 | 25533‐2010 |
| Plumbous (Pb), (mg/kg) | 1.2 | 1.3 | ≦5.0 | GB 5009.12 |
| Total galacturonic acid, (w/%) | 68.0 | 67.0 | ≥65.0 | Ultraviolet spectrophotometer method |
| Total percent of ash, (w/%) | 1.3 | 3.5 | / | GB5009.4‐2010 |
Above-mentioned detected result shows, according to the every main physical and chemical index of embodiment 2 products obtained therefrom and control sample all meet Guo Biao ?the requirement of pectin, prove that the present invention can produce qualified beet pectin.As shown in table 1, the extraction yield of the beet pectin adopting the present invention to produce is 11.7%, and the beet pectin extraction yield that prior art (traditional hot acid method) obtains is only 10.3%.This shows, under the identical condition such as extraction pH, Extracting temperature, solid-liquid ratio, to be significantly better than prior art in the present invention in extraction yield.From the above results, Xi Suan of the present invention ?pressurised extraction method can effectively improve the extraction yield of beet pectin and shorten extraction time.Compared with prior art, why the present invention can accelerate the extraction efficiency of beet pectin, may mainly come from following reason: 1) pressure treatment facilitates the peptization of pectin, have compressed the pectous duct of beet cell pars intramuralis bag, pectin is made to be squeezed to external diffusion, thus accelerate mass transfer velocity, and finally improve the extraction efficiency of beet pectin.
In food system, pectin is commonly used for emulsifying agent and thickening material, for preventing oil droplet and aqueous phase layering and improving the quality of food.The effect that beet pectin can reach is directly related with the concentration of beet pectin, and under same amount, the effective concentration of beet pectin depends on the purity of beet pectin.Therefore, under the condition of equal consumption, the purity improving beet pectin can improve the result of use of beet pectin.As shown in table 1, Xi Suan provided by the invention ?the total ash content of product that obtains of pressurised extraction method be only 1.3%, significantly lower than control sample 3.5%.The content of total percent of ash is directly proportional to the content of non-pectin composition.Therefore, method of the present invention can obtain the higher beet pectin product of purity.As can be seen from the above embodiments, use method of the present invention, the beet pectin that extraction yield is high, purity is high can be prepared.
The weight average of table 2 Different Extraction Method gained beet pectin, number-average molecular weight and polydispersity coefficient
As shown in table 2, art methods due to extraction time longer, the sour unstable structure such as pectin molecule neutral sugar especially on side chain is easily degraded in leaching process, causes the molecular weight and molecular weight of pectin, polydispersity coefficient to raise.
Fig. 1 is the pectin sample molecular weight distribution curve adopting Size Exclusion Chromatograph SEC-rheological properties technology to record.Therefrom can find out, compared with experiment sample, the absorption peak of control sample is wider, and appearance time totally offsets backward, and this reflects the degraded that pectin molecule receives in leaching process indirectly; Absorption peak broadens and also shows that the homogeneity degree of product reduces.The result of associative list 2 and Fig. 1 can draw, Xi Suan provided by the invention ?pressurised extraction method can shorten extraction time of beet pectin, and reduce the palliating degradation degree of beet pectin in hot acid environment, thus keep integrity and the homogeneity of pectin molecule structure.
As mentioned above, beet pectin of the present invention preparation is raw material with the wet dregs of rice, need not dry, pulverizes, sieve, can very big simplification of flowsheet, minimizing significant loss; Diluted acid hydrolysis lixiviate, condition is relatively gentle, can reduce pectin molecule degraded; In extracting solution except beet pectin, filter residue is mainly Mierocrystalline cellulose and the hemicellulose raw material as processing food fibre further, and filtrate is mainly water-soluble carbohydrate, and diluted acid and ethanol are recycled, environmentally safe.
The method adopted in Fig. 1, table 1 and table 2 index determining:
1. galactouronic acid detection method
With trihydroxy-biphenyl for developer, ultraviolet spectrophotometer method is adopted to measure the galacturonic acid content of beet pectin.
(1) foundation of typical curve: with D ?galacturonic acid for standard control, be mixed with the D ?galacturonic acid solution of different mass concentration, in the sample getting each 0.4mL different concns and 10mL test tube with ground stopper, be placed in ice bath and cool 5min, blank tube deionized water replaces.Getting 2.4mL sulfuric acid (containing 0.0125M sodium tetraborate) adds in test tube, and after abundant shaken well, develop the color 5min in 100 DEG C of boiling water.Colour developing terminates to be cooled to room temperature rapidly, then adds the developer of 40 μ L, and at room temperature develop the color after shaken well 15min, then measures the absorbancy of solution at 520nm.Blank tube 0.5% sodium hydroxide solution of 40 μ L replaces.Typical curve as shown in Figure 1.
(2) sample determination: precise 5mg beet pectin sample, dissolves in deionized water, and is settled to 100mL with deionized water.Get in 0.4mL sample and 10mL test tube with ground stopper, carry out heating according to identical method, develop the color, absorbance measurement.Galacturonic acid content calculates according to the following formula:
In above formula:
A represents the absorbancy of testing sample;
M represents the content of beet pectin, mg;
V0 represents the volume of pectin diluting soln to be measured, mL;
V represents constant volume, mL.
2. pectin extraction rate method of calculation
Get constant weight, clean aluminum dish, by the weight of ten thousand/analytical balance record aluminum dish, then take 2 ~ 3g beet pulp, beet pectin, be placed in hot air drier, dry 10h at 105 DEG C.Aluminum dish is placed in moisture eliminator immediately and cools after taking out from loft drier, be down to until temperature the weight weighing aluminum dish after room temperature.Beet pectin extraction yield calculates according to formula 2:
In above formula:
M
1represent the weight of beet pulp, dry basis, g;
M
2represent the weight of beet pectin, dry basis, g.
3. molecular weight, the molecular weight distribution curve measuring method of beet pectin
Adopt high performance liquid phase Size Exclusion Chromatograph SEC associating multi-angle laser light scattering (HPSEC ?MASL) to measure the molecular weight of beet pectin, chromatographic condition is as follows: moving phase, 50mM SODIUMNITRATE (containing 0.05% sodiumazide); Flow velocity is 0.6mL/min; Chromatographic column is Ultrahydrogel Series of Water solubleness gel post guardcolumn, Linear and 1000 (three series connection), and separating ranges is 10
3~ 10
8g/mol; Test sample concentration is 1mgmL
?1; Dn/dc value is 0.146mLg
?1; Analysis temperature 35 DEG C; Sampling volume 100 μ L.
Detector: DAWNHELEOS II multi-angle laser detector; OptilabT ?rEX Composition distribution.
Claims (6)
1. rare sour ?pressurization prepares a method for beet pectin, it is characterized in that comprising the following steps:
Step 1: by percentage to the quality, the wet dregs of rice with moisture 75 ~ 80% are raw material, freeze slow for the freezing pressure dregs of rice of the fresh pressure dregs of rice or storage, clear water washing, drain rear loading extractor, add deionized water; Controlling the pressure dregs of rice with deionized water quality than being 1:10 ?20, is 2.0 ?3.4 by the pH value of acid-conditioning solution; The acid added is hydrochloric acid, sulfuric acid, nitric acid or citric acid, soaks at 65 ~ 85 DEG C;
Step 2: boost to 0.1 ~ 1.0Mpa to the extractor cycle in immersion process, keeps pressure 1 ~ 5min, after being returned to normal pressure 20 ~ 60min, then to boost and decompression is returned to normal pressure; The pressure alteration cycle is 1 ~ 3 time, and total soak time is 100 ~ 180min, comprises the pressure alteration cycle;
Step 3: stir material in extractor, controls stirring velocity 60 ~ 200rpm, obtains coarse filtration liquid;
Step 4: add carbonate solution in coarse filtration liquid, makes the suspended substance in precipitation and absorption coarse filtration liquid and calcium and magnesium salt ash content, and extracting solution, after mechanical deslagging device precipitation separation and suspended substance, enters pressure filter and carries out essence filtration; The carbonate added is CaCO
3or Na
2cO
3, add-on is make the pH value of coarse filtration liquid be 2.5 ~ 4.5;
Step 5: the pectin extracting soln after essence being filtered concentrates, gradually in the pectin solution after concentrated adds ethanol, and pectin is dewatered precipitating; Alcohol analyse after through extruding or centrifugal desolvation, drying obtains beet pectin.
2. Xi Suan according to claim 1 ?pressurization prepare the method for beet pectin, it is characterized in that, described pressure filter is butterfly or plate and frame(type)filter press.
3. Xi Suan according to claim 1 ?pressurization prepare the method for beet pectin, it is characterized in that, the described gradual alcoholic solvent that adds is that the different liquid level point analysing tank at alcohol instill ethanolic soln simultaneously, and the quality adding ethanol is 3 ~ 5 times of pectin solution.
4. Xi Suan according to claim 1 ?pressurization prepare the method for beet pectin, it is characterized in that, the mass concentration of described ethanolic soln is 75 ~ 95%.
5. Xi Suan according to claim 1 ?pressurization prepare the method for beet pectin, it is characterized in that, described concentrated be that at 40 ~ 60 DEG C of temperature, evaporate removing moisture be 1 ~ 5% to containing solid quality percentage composition for pectin extracting soln after essence being filtered; Concentrated vaporizer is that single-action or multiple-effect series combination form.
6. Xi Suan according to claim 1 ?pressurization prepare the method for beet pectin, it is characterized in that, described drying refer to by the solid wet pectin of centrifugal exsolution agent at <60 DEG C of temperature dry obtain water content lower than 10% pectin.
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| CN105924542A (en) * | 2016-06-22 | 2016-09-07 | 平凉市佳禾农产品加工有限责任公司 | Method for extracting pectin from glutinous corn |
| CN106622157A (en) * | 2016-12-20 | 2017-05-10 | 华南理工大学 | Modified beet pulp fiber adsorbent as well as preparation method and application thereof |
| CN116217755A (en) * | 2022-12-07 | 2023-06-06 | 广东省人民医院 | Cordyceps sinensis glycogen content detection method and structure detection method |
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| CN116217755A (en) * | 2022-12-07 | 2023-06-06 | 广东省人民医院 | Cordyceps sinensis glycogen content detection method and structure detection method |
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