CN107019222A - The extraction process of anti-oxidation active substance and the preparation technology of antioxidation activity particle - Google Patents
The extraction process of anti-oxidation active substance and the preparation technology of antioxidation activity particle Download PDFInfo
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- CN107019222A CN107019222A CN201710240859.3A CN201710240859A CN107019222A CN 107019222 A CN107019222 A CN 107019222A CN 201710240859 A CN201710240859 A CN 201710240859A CN 107019222 A CN107019222 A CN 107019222A
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- active substance
- oxidation active
- lotus rhizome
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- 239000013543 active substance Substances 0.000 title claims abstract description 89
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to field of health care food, and the extraction process and the preparation technology of antioxidation activity particle of more particularly to a kind of anti-oxidation active substance.The extraction process of anti-oxidation active substance, it comprises the following steps：Lotus rhizome slurry is carried out homogeneous using the homogeneous method of high speed and extracted.Wherein, the rotating speed of the homogeneous method of high speed is 6000~14000r/min, and the temperature of extraction is 50~90 DEG C.The extraction process is destroyed the intact cell configuration of lotus rhizome using the powerful machinery of the homogeneous method of high speed and fluid shear, is extracted beneficial to anti-oxidation active substance in lotus rhizome.The extraction efficiency for further improving anti-oxidation active substance is pre-processed to lotus rhizome, while the species that perfect anti-oxidation active substance is extracted.
Description
Technical field
The present invention relates to field of health care food, and the extraction process of more particularly to a kind of anti-oxidation active substance and anti-oxidant
The preparation technology of active particle.
Background technology
Lotus rhizome is Nymphaeceae nelumbium, is a kind of important economic aquatic plant, has concurrently and view and admire, eat and medicinal work(
Effect, consumers in general are liked.In China, lotus rhizome is mainly distributed on the province such as Hubei, Hunan, Zhejiang and Guangdong, is China's plantation
Area is maximum, yield highest aquatic vegetable.Lotus rhizome contains abundant carbohydrate, starch, protein, tannin, lipid etc.
Composition, in addition, also rich in various active composition, such as dietary fiber, flavones, volatile oil, alkaloid, polysaccharide, with anti-
Inflammation, lipid-loweringing, hypotensive, hypoglycemic, anti-AIDS and bacteriostasis etc., there is wide DEVELOPMENT PROSPECT.Wherein Nelumbo nucifera extract table
Reveal the multiple biological activities such as antibacterial, antitumor, anti-oxidant, hypoglycemic and immunological regulation, more gone out with its antioxidation activity especially
Main antioxidant component in crowd, lotus rhizome is polysaccharide and phenolic compound.
At present, the Study on extraction on lotus rhizome antioxidant content focuses on a certain class material mostly.It is many in such as lotus rhizome
The ultrasonic assistant Study on extraction of Flavonoid substances, lotus rhizome reclaimed water in the extraction technical study of aldehydes matter, lotus rhizome
Study on extraction of soluble polysaccharide etc., but then had no for the collaboration Study on extraction of water-soluble antioxidant content in lotus rhizome
Report.
The content of the invention
It is an object of the invention to provide a kind of extraction process of anti-oxidation active substance, its can fast and efficiently from
The bioactive substance of antioxidant effect is extracted in lotus rhizome.
Another object of the present invention is to provide a kind of preparation technology of antioxidation activity particle, quickly will by the technique
Extract obtained anti-oxidation active substance and be prepared as particle, its operating condition is gentle, it is easy to accomplish.
The present invention is solved its technical problem and realized using following technical scheme：
The present invention proposes a kind of extraction process of anti-oxidation active substance, and it comprises the following steps：By lotus rhizome slurry using high
The homogeneous method of speed is carried out homogeneous and extracted.Wherein, the rotating speed of the homogeneous method of high speed is 6000~14000r/min, and the temperature of extraction is
50~90 DEG C.
The present invention proposes a kind of preparation technology of antioxidation activity particle, and it comprises the following steps：Above-mentioned antioxidation activity
The extraction process of material extracts anti-oxidation active substance.Anti-oxidation active substance is spray-dried and wet granulation.
The beneficial effect of the extraction process of the anti-oxidation active substance of the embodiment of the present invention is：It is anti-oxidant that the present invention is provided
The extraction process of active material is broken the intact cell configuration of lotus rhizome using the powerful machinery of the homogeneous method of high speed and fluid shear
It is bad, extracted beneficial to anti-oxidation active substance in lotus rhizome.Lotus rhizome is pre-processed and further improves anti-oxidation active substance
Extraction efficiency, while the species that perfect anti-oxidation active substance is extracted.And extracted by the way of heating, accelerate anti-
The speed and content of active oxide material dissolving in a solvent, improve the efficiency and speed of extraction.Extraction is resisted
Active oxide material is prepared as particle, extends the storage time of anti-oxidation active substance, while easy to carry and transport.
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, are the conventional production that can be obtained by commercially available purchase
Product.
In the description of the invention, it is necessary to which explanation, term " first ", " second " etc. are only used for distinguishing description, without
It is understood that to indicate or imply relative importance.
Below to the extraction process and the preparation work of antioxidation activity particle of the anti-oxidation active substance of the embodiment of the present invention
Skill is specifically described.
A kind of extraction process of anti-oxidation active substance provided in an embodiment of the present invention：
S1, lotus rhizome pretreatment
The lotus rhizome surface attachment sludge just dug out, it is impossible to be used directly to extract anti-oxidation active substance, meanwhile, entirely
Lotus rhizome volume is excessive to be unfavorable for extracting anti-oxidation active substance, accordingly, it would be desirable to be pre-processed to lotus rhizome.
First, lotus rhizome is dried standby after lotus rhizome to be cleaned to the rubbish such as the sludge to remove lotus rhizome surface.Dry be in order to
Extend the quality guarantee period of lotus rhizome, it is to avoid lotus rhizome moisture is too high and causes to go mouldy, increase the loss of lotus rhizome.Then by lotus rhizome
Progress, which is crushed, obtains lotus rhizome slurry, and crushing is to reduce the volume of lotus rhizome, be easy to subsequent extracted.Meanwhile, crushing can destroy lotus
The cell membrane of lotus root cell, beneficial to the dissolution of the anti-oxidation active substance in lotus rhizome.
Preferably, the lotus rhizome after cleaning is freezed successively, crushed and defrosting processing.Lotus rhizome is freezed, powder
Broken and thaw processing rather than directly crushing extraction, the anti-oxidation active substance for being able in lotus rhizome to contain are complete
Extract.Lotus rhizome is freezed first so that the hydrone in lotus rhizome condenses into small ice slush and so that lotus rhizome is hardened, is easy to follow-up
Crushing, while freezing can effectively prevent the anti-oxidation active substance in lotus rhizome from changing.It is former during subsequent extracted
The water for becoming ice slush in material is changed into aqueous water again, to provide sufficient moisture, the antioxidation activity being more convenient in lotus rhizome
Material dissolution.
It is further preferred that the temperature of freezing is -30--18 DEG C, cooling time is 24-48h.In this temperature and this freezing
In time range, the water in lotus rhizome can fully be condensed into ice slush, and will not freezing raw material.Ensureing the base of refrigerating effect
The structure of the anti-oxidation active substance in lotus rhizome is ensure that on plinth to be destroyed, and then ensure that the antioxygen of antioxidation activity thing
Change performance.
Lotus rhizome is crushed after freezing, the structure of the cell in destruction lotus rhizome, be that cell is broken by complete clasmatosis
Piece, the dissolution of intracellular anti-oxidation active substance when being easy to subsequent extracted.
Lotus rhizome is thawed after crushing, now, initially the ice slush that is formed melts as water again during freezing, and now lotus
Each cell in lotus root is no longer complete, and cell membrane is destroyed, intracellular to save water, therefore, intracellular water after defrosting
Distribute cell so that each lotus rhizome is changed into solidliquid mixture, the water after these thawings can be carried further for subsequent extracted
For solvent, the dissolution for the anti-oxidation active substance for being dissolved in water being easy in lotus rhizome, meanwhile, antioxygenic activity in perfect extraction process
The species of material.And thaw and enhance follow-up homogeneous effect, further, it is ensured that extraction effect.
Most of anti-oxidation active substances easily react with the yang-energy in air and then influence it under the high temperature conditions
The activity function of antioxygen.Therefore, in order to which the temperature for the antioxidant effect defrosting for ensureing anti-oxidation active substance in lotus rhizome is room
Temperature.Meanwhile, thawing time is long, and the anti-oxidation active substance being initially defrosted in successful lotus rhizome easily changes, therefore, lotus
The thawing time of lotus root is 4-6 hours.Thawing time and thaw point both ensure that the defrosting effect of lotus rhizome in the range of this, and to the greatest extent might be used
Reduce the possibility that anti-oxidation active substance in lotus rhizome changes energy.
S2, high speed are homogeneous to decompose
Carried out after the lotus rhizome obtained after crushing slurry is mixed with deionized water homogeneous.Homogeneous rotating speed be 6000~
14000r/min, the homogeneous time is 5-8 minutes.Carried out using above-mentioned high rotating speed homogeneous, using the teaching of the invention it is possible to provide stronger machinery and liquid
Lotus rhizome, can be not only well mixed by body shearing force using above-mentioned rotating speed with deionized water, can utilize powerful shearing force
By the eucaryotic cell structure destruction in lotus rhizome, and then beneficial to the extraction of the antioxygenic activity material in lotus rhizome.Also, using the high rotating speed more
It is intracellular into what is crushed beneficial to the hydrone in deionized water, improve extraction efficiency.And ensure that using the homogeneous time
Deionized water is sufficiently well mixed with lotus rhizome slurry, the extraction of anti-oxidation active substance more conducively in lotus rhizome.Meanwhile, when this is homogeneous
In ensure that lotus rhizome slurry in cell damaged degree, prevent because homogeneous overlong time cause part be dissolved it is anti-oxidant
The performance reduction of active material.Deionized water is added, is the antioxidation activity in being starched beneficial to lotus rhizome to provide sufficient solvent
The dissolution of material.Further, deionized water and the mass ratio that lotus rhizome is starched are 10-20:1.Now, anti-oxidation active substance is carried
Efficiency is taken to reach most preferably.
S3, extraction
Lotus rhizome after will be homogeneous is starched leaches what is contained to extract in lotus rhizome slurry within 1-4 hours in 50~90 DEG C of water bath with thermostatic control
Anti-oxidation active substance.Anti-oxidation active substance is extracted by the way of heating, is because temperature rise solubility rise, is beneficial to
Anti-oxidation active substance dissolution in lotus rhizome slurry.And the Extracting temperature used is 50~90 DEG C, in the temperature range in lotus rhizome
Anti-oxidation active substance stripping quantity is at most and dissolution rate is most fast, and obtained anti-oxidation active substance will not be also oxidized.Take
It is that slurry is constantly in a stable state, more conducively anti-oxidant work because constant temperature ensure that lotus rhizome that the mode of constant temperature, which extract,
The dissolution of property material.And be then the mode of heatings such as relative oil bath or sand-bath using water-bath, water-bath is more easy to operate, is more easy to reach perseverance
The effect of temperature, and then the more conducively extraction of anti-oxidation active substance.Extraction time is that ensure that within 1-4 hours antioxygen in lotus rhizome slurry
Change the extraction effect of active material, while can prevent the reducing substanceses such as anti-oxidation active substance and air from reacting, and then lose
Go oxidation resistant activity.
S4, post processing
Lotus rhizome slurries after the completion of extraction are subjected to centrifugal treating and obtain supernatant.Anti-oxidation active substance in extraction process
In dissolving and solvent, and remaining insoluble matter (water insoluble material) is still existed in lotus rhizome slag, and then carries out solid-liquid point
From obtaining the only liquid containing anti-oxidation active substance, and remove other impurities, it is ensured that anti-oxidation active substance in solvent
Content and purity.It is simple, conveniently using centrifugally operated, and more other solid-liquid separating method separating effects are more preferably.Enter one
Preferably, the rotating speed of centrifugation is 3500-4500r/min to step, and centrifugation time is 5-8 minutes.
Only contain solvent in anti-oxidation active substance, but supernatant in obtained supernatant too high, and then cause anti-
Active oxide material content is too low, it is impossible to play good health-care effect.Therefore, in order to lift containing for anti-oxidation active substance
Amount, it is ensured that its oxidation resistant effect, the embodiment of the present invention is concentrated supernatant, obtains anti-oxidation active substance levels
Moderate concentrate.Particularly supernatant is concentrated in Rotary Evaporators, the multiple of concentration is 3-5 times, that is, is obtained
Concentrate amount be supernatant 1/3-1/5.Anti-oxidation active substance containing moderate concentration in the concentrate, that is, complete
The extraction of anti-oxidation active substance in lotus rhizome.
The preparation technology for the antioxidation activity particle that said extracted is obtained is liquid, though also can be used directly and with antioxygen
Effect of change, but liquid is difficult to store, is difficult transport, and it is not portable.
Therefore, extraction is obtained into anti-oxidation active substance in order to solve this technical problem embodiment of the present invention to be granulated
Particle is obtained, is easy to carry, transports.Therefore, the embodiment of the present invention also provides a kind of preparation technology of antioxidation activity particle：It is first
First the anti-oxidation active substance in lotus rhizome is then granulated, specific preparation technology is as follows according to above-mentioned extracting method：
S1, spray drying：
The above-mentioned concentrate obtained after concentration is passed through in spray drying tower and is dried and then obtains extract powder.Will
Concentrate is dried to remove the moisture in concentrate, it is ensured that the softwood of follow-up antioxidation activity particle can be smoothly prepared into
Arrive, while ensure that the content of anti-oxidation active substance in antioxidation activity particle is suitable.The air intake of specific spray drying tower
Temperature is 160~180 DEG C, 75~85 DEG C of leaving air temp, peristaltic pump 200~300r/min of charging rate, compressed air pressure
0.2-0.5MPa.Concentrate is dried using above-mentioned EAT, heat exchange can be quickly realized, and then reach quick
Dry purpose.Meanwhile, control leaving air temp, peristaltic pump charging rate, compressed air pressure are to pay mental in inventor
That discusses out afterwards can ensure that the condition that drying effect can avoid anti-oxidation active substance property from changing again.
S2, prepare softwood；
By extract powder, sucrose and dextrin according to mass ratio be 1:2-3:0.8-1.2 ratios are mixed.Wherein sucrose is played
The effect of the seasoning of one filling, and dextrin is then to be used as adhesive so that it can be bonded together between extract powder and extract powder
The slightly larger particle of particle diameter is formed, while sucrose is filled, it is ensured that contain suitable extract powder in every particle.
It is ground after mixing, to control the particle diameter of particle, while so that extract powder, sucrose and dextrin are easier to be bonded in
Together.Easily mixed with 90-95% ethanol after grinding.Ethanol mainly moistens extract powder, dextrin and sucrose, and then causes dextrin
Play the effect of adhesive.
The softwood prepared is pelletized by nylon mesh screen, and the mesh mesh number of nylon wire is 16-18 mesh.Using nylon
Mesh screen is, in order to prevent metal fillings (disconnected silk) or disconnected stainless steel wire brings particle into, and then to influence the matter of antioxidation activity particle
Amount, hinders anti-oxidation active substance to play its effect.Meanwhile, it is controller tightness installing screen cloth, screen cloth pine excessively may
Sticky, the obtained wet granular for increasing softwood is thick and tight.Conversely, obtained particle is carefully loose.And the mesh of 16-18 mesh is used,
It ensure that the suitable size of wet granular, it is ensured that the size of the particle subsequently prepared, and ensure that each in every particle
The content of active ingredient is suitable.
Then wet granular is dried 1.5-2 hours under conditions of 60-80 DEG C, dried to remove the moisture in wet granular,
So that will not be glued between particle and particle glutinous.Dried particle is then passed through into nylon mesh screen again so that caking, of adhesion
Grain scatters, and screens out fine powder, obtains the single-size with certain particle size.
The antioxidation activity particle prepared is in brown color, dry, uniform, color and luster it is consistent, no moisture absorption, softening, caking,
The phenomenons such as deliquescence, free from extraneous odour is in good taste, micro- sweet tea.The inspection of moisture, granularity and melting：The water of particle obtained by wet granulation
Point content is low, and epigranular, melting is good, meets the regulation of Chinese Pharmacopoeia.
A kind of extraction process for anti-oxidation active substance that the present invention is provided utilizes the homogeneous method of high speed, utilizes its powerful machine
Lotus rhizome eucaryotic cell structure is destroyed complete by tool and liquid shear effect, beneficial to the dissolution of anti-oxidation active substance.Using freezing, powder
Broken, method that is thawing is pre-processed the dissolution of antioxidation activity in more conducively lotus rhizome to lotus rhizome, increases dissolution efficiency, together
When, expand the species of the anti-oxidation active substance of extraction, the further perfect activity of anti-oxidation active substance.Meanwhile, will
Extract obtained anti-oxidation active substance and be prepared as particle, be easy to the storage and carrying of anti-oxidation active substance, expand anti-
The use scope of active oxide material.
The feature and performance to the present invention are described in further detail with reference to embodiments.
Embodiment 1
The present embodiment provides a kind of extraction process of anti-oxidation active substance：
After the lotus rhizome of fresh harvesting is cleaned, under conditions of -18 DEG C, freeze 36 hours, then crushed, thaw,
The temperature of defrosting is room temperature, and the time of defrosting is 4 hours.Lotus rhizome after crushing is with deionized water using mass ratio as 15:1 ratio
With homogeneous 6 minutes of 10000r/min high rotating speed after being mixed.Then leached 2 hours in 80 DEG C of water bath with thermostatic control.Then
Carry out centrifugal treating and obtain supernatant, the rotating speed of centrifugation is 3500r/min, and centrifugation time is 5 minutes.Finally supernatant is carried out
Concentration, the multiple of concentration is 4 times.Complete the extraction of anti-oxidation active substance.
The present embodiment also provides a kind of preparation technology of antioxidation activity particle：
Concentrate obtained above be spray-dried to obtain extract powder, the EAT of specific spray drying tower is
160 DEG C, 80 DEG C of leaving air temp, peristaltic pump charging rate 300r/min, compressed air pressure 0.2MPa.Then by extract powder and sugarcane
The mass ratio of sugar, dextrin mixing, wherein extract powder and sucrose, dextrin is 1:2:0.8.The ethanol for adding 90% in mixture again enters
Row, which is mixed and soaked, obtains softwood, and softwood then is prepared into wet granular by 16 mesh nylon wires sieve.Wet granular is at 60 DEG C
Under the conditions of dry 1.5 hours after antioxidation activity particle is obtained by nylon mesh screen again.
Embodiment 2
The present embodiment provides a kind of extraction process of anti-oxidation active substance：
After the lotus rhizome of fresh harvesting is cleaned, under conditions of -24 DEG C, freeze 48 hours, then crushed, thaw,
The temperature of defrosting is room temperature, and the time of defrosting is 6 hours.Lotus rhizome after crushing is with deionized water using mass ratio as 10:1 ratio
With homogeneous 8 minutes of 14000r/min high rotating speed after being mixed.Then leached 4 hours in 90 DEG C of water bath with thermostatic control.Then
Carry out centrifugal treating and obtain supernatant, the rotating speed of centrifugation is 4000r/min, and centrifugation time is 8 minutes.Finally supernatant is carried out
Concentration, the multiple of concentration is 3 times.Complete the extraction of anti-oxidation active substance.
The present embodiment also provides a kind of preparation technology of antioxidation activity particle：
Concentrate obtained above be spray-dried to obtain extract powder, the EAT of specific spray drying tower is
180 DEG C, 75 DEG C of leaving air temp, peristaltic pump charging rate 250r/min, compressed air pressure 0.5MPa.Then by extract powder and sugarcane
The mass ratio of sugar, dextrin mixing, wherein extract powder and sucrose, dextrin is 1:2.5:1.The ethanol for adding 93% in mixture again enters
Row, which is mixed and soaked, obtains softwood, and softwood then is prepared into wet granular by 18 mesh nylon wires sieve.Wet granular is at 70 DEG C
Under the conditions of dry 2 hours after antioxidation activity particle is obtained by nylon mesh screen again.
Embodiment 3
The present embodiment provides a kind of extraction process of anti-oxidation active substance：
After the lotus rhizome of fresh harvesting is cleaned, under conditions of -30 DEG C, freeze 24 hours, then crushed, thaw,
The temperature of defrosting is room temperature, and the time of defrosting is 5 hours.Lotus rhizome after crushing is with deionized water using mass ratio as 20:1 ratio
With homogeneous 5 minutes of 12000r/min high rotating speed after being mixed.Then leached 3 hours in 50 DEG C of water bath with thermostatic control.Then
Carry out centrifugal treating and obtain supernatant, the rotating speed of centrifugation is 4500r/min, and centrifugation time is 6 minutes.Finally supernatant is carried out
Concentration, the multiple of concentration is 5 times.Complete the extraction of anti-oxidation active substance.
The present embodiment also provides a kind of preparation technology of antioxidation activity particle：
Concentrate obtained above be spray-dried to obtain extract powder, the EAT of specific spray drying tower is
170 DEG C, 85 DEG C of leaving air temp, peristaltic pump charging rate 200r/min, compressed air pressure 0.3MPa.Then by extract powder and sugarcane
The mass ratio of sugar, dextrin mixing, wherein extract powder and sucrose, dextrin is 1:3:1.2.The ethanol for adding 95% in mixture again enters
Row, which is mixed and soaked, obtains softwood, and softwood then is prepared into wet granular by 17 mesh nylon wires sieve.Wet granular is at 80 DEG C
Under the conditions of dry 2 hours after antioxidation activity particle is obtained by nylon mesh screen again.
Embodiment 4
The present embodiment provides a kind of extraction process of anti-oxidation active substance：
After the lotus rhizome of fresh harvesting is cleaned, under conditions of -26 DEG C, freeze 30 hours, then crushed, thaw,
The temperature of defrosting is room temperature, and the time of defrosting is 6 hours.Lotus rhizome after crushing is with deionized water using mass ratio as 17:1 ratio
With homogeneous 7 minutes of 8000r/min high rotating speed after being mixed.Then leached 2 hours in 60 DEG C of water bath with thermostatic control.Then enter
Row centrifugal treating obtains supernatant, and the rotating speed of centrifugation is 4200r/min, and centrifugation time is 7 minutes.Finally supernatant is carried out dense
Contracting is handled, and the multiple of concentration is 5 times.Complete the extraction of anti-oxidation active substance.
The present embodiment also provides a kind of preparation technology of antioxidation activity particle：
Concentrate obtained above be spray-dried to obtain extract powder, the EAT of specific spray drying tower is
165 DEG C, 77 DEG C of leaving air temp, peristaltic pump charging rate 220r/min, compressed air pressure 0.4MPa.Then by extract powder and sugarcane
The mass ratio of sugar, dextrin mixing, wherein extract powder and sucrose, dextrin is 1:2.7:0.9.Added again in mixture 92% ethanol
Mixed and soaked and obtain softwood, softwood is then prepared into wet granular by 16 mesh nylon wires sieve.Wet granular is at 75 DEG C
Under conditions of dry 2 hours after antioxidation activity particle is obtained by nylon mesh screen again.
Embodiment 5
The present embodiment provides a kind of extraction process of anti-oxidation active substance：
After the lotus rhizome of fresh harvesting is cleaned, under conditions of -20 DEG C, freeze 42 hours, then crushed, thaw,
The temperature of defrosting is room temperature, and the time of defrosting is 6 hours.Lotus rhizome after crushing is with deionized water using mass ratio as 15:1 ratio
With homogeneous 8 minutes of 14000r/min high rotating speed after being mixed.Then leached 1 hour in 70 DEG C of water bath with thermostatic control.Then
Carry out centrifugal treating and obtain supernatant, the rotating speed of centrifugation is 3700r/min, and centrifugation time is 7 minutes.Finally supernatant is carried out
Concentration, the multiple of concentration is 3 times.Complete the extraction of anti-oxidation active substance.
The present embodiment also provides a kind of preparation technology of antioxidation activity particle：
Concentrate obtained above be spray-dried to obtain extract powder, the EAT of specific spray drying tower is
170 DEG C, 82 DEG C of leaving air temp, peristaltic pump charging rate 280r/min, compressed air pressure 0.5MPa.Then by extract powder and sugarcane
The mass ratio of sugar, dextrin mixing, wherein extract powder and sucrose, dextrin is 1:2.2:1.1.Added again in mixture 95% ethanol
Mixed and soaked and obtain softwood, softwood is then prepared into wet granular by 16 mesh nylon wires sieve.Wet granular is at 77 DEG C
Under conditions of dry 1.5 hours after antioxidation activity particle is obtained by nylon mesh screen again.
Experimental example 1
Detect polyoses content
Total sugar content is measured using phend-sulphuric acid, and reduced sugar is determined with DNS methods, and polyoses content=total reducing sugar contains
Amount-content of reducing sugar.
Total sugar content is determined
The principle that total sugar content is determined be polysaccharide under effect of sulfuric acid, be first hydrolyzed into monose, and be dehydrated generation alditol and derive
Thing, generates orange solution with phenol, has absorption maximum at 490nm, absorption value is linear with sugared content.
Reagent：The concentrated sulfuric acid (analysis is pure), 5% phenol solution (analysis is pure), standard glucose.
Operating procedure：
Make standard curve：It is accurate to weigh 105 DEG C of drying and be made into 500mL volumetric flasks to the glucose 50mg of constant weight
0.1mg/mL standard liquid, be made into 0 respectively with distilled water, 0.02,0.04,0.06,0.08,0.1mg/mL.1mL titers point
5% phenol solution 1mL is not added, and is rapidly added concentrated sulfuric acid 5mL, stands 10min.Shake up, 30 DEG C place 30min after
490nm determines OD values, using glucose content as abscissa, and OD values are ordinate, and making obtains standard curve.
2. sample size is determined：1.0mL embodiments 1-5 extract solution is drawn respectively, according to above-mentioned steps time-and-motion study
OD490, and substitute into the total sugar content that standard curve calculates sample.
Content of reducing sugar is determined
Under the principle that content of reducing sugar is determined is alkalescence condition, DNS can generate 3- amino -5- nitro water with reducing sugar reaction
Poplar acid.This material is into brownish red under boiling conditions, and shade is linear with content of reducing sugar within the specific limits.
Content of reducing sugar can be determined by determining OD values accordingly.
Reagent and preparation：Concentration is 1mg/mL glucose standards solution.DNS nitrite ions：3.25gDNS is weighed to be dissolved in less
In amount distilled water and it is transferred in 500mL volumetric flasks, adds 2mol/L NaOH solution 162.5mL, add the third the three of 7.5mL
Alcohol, shakes up, plus distilled water is settled to 500mL, shakes up.
Operating procedure：
1. standard curve making：1mg/mL glucose standards solution 0.00mL, 0.10mL are separately added into 6 test tubes,
0.20mL, 0.30mL, 0.40mL, 0.50mL, mend distilled water to 0.5mL, then add DNS reagent 1.5mL, fully mix.Put
5min is boiled in boiling water bath, then rapid flowing water cooling, and add 4mL distilled water into test tube respectively, mixed.In 540nm
Read OD values in place.With concentration of glucose (μ g/mL) for abscissa, standard curve is made by ordinate of OD values, is obtained its linear time
Return equation.
2. sample size is determined：0.5mL embodiments 1-5 extract solution is drawn respectively, according to above-mentioned steps time-and-motion study OD
Value, and the content of reducing sugar calculated in sample is substituted into regression equation.
The polyoses content testing result of embodiment 1- embodiments 5 is shown in Table 1.
The polyoses content testing result of table 1
| Polyoses content | |
| Embodiment 1 | 2.7mg/mL |
| Embodiment 2 | 3.0mg/mL |
| Embodiment 3 | 2.9mg/mL |
| Embodiment 4 | 2.7mg/mL |
| Embodiment 5 | 2.9mg/mL |
Experimental example 2
Total phenol content is determined
Specific total phenol content assay method refers to " guard the folin's methods measure of total phenol content in thunder, Wang Qing chapter rhizoma nelumbinis rigidly
[J] Hua Zhong Agriculture University journal, 2003,22 (4)：412-414”.Measurement result is shown in Table 2.
The total phenol content measurement result of table 2
| Polyoses content | |
| Embodiment 1 | 32μg/mL |
| Embodiment 2 | 34μg/mL |
| Embodiment 3 | 34μg/mL |
| Embodiment 4 | 33μg/mL |
| Embodiment 5 | 32μg/mL |
Experimental example 3
Determination of total flavonoids
Measuring principle is the 3- hydroxyls in flavone compound, 4- hydroxyls or 5- hydroxyls, 4- carbonyls or adjacent two phenolic hydroxyl groups,
Complex reaction is carried out with aluminium salt, the complex compound of red is generated in the basic conditions.With AAS in measure under 500nm wavelength
Its absorbance, is compared with rutin standard reference material, carries out the quantitative determination of general flavone in determinand.
Operating procedure：
The preparation of standard curve：Precision measures reference substance solution 1mL, 2mL, 3mL, 4mL, 5mL and 6mL, and 25mL is put respectively
In measuring bottle, 6mL, plus 5% sodium nitrite solution 1mL are respectively added water to, is mixed, is placed 6 minutes, plus 10% aluminum nitrate solution 1mL, mix
It is even, place 6 minutes, plus 4% sodium hydroxide solution 10mL, add water to scale, shake up, place 15 minutes, with corresponding reagent
Make blank, according to UV-VIS spectrophotometry, absorbance is determined at 500nm wavelength, using reference substance concentration as abscissa,
Absorbance is ordinate, draws standard curve.
Sample is determined：The accurate extract solution 10mL for measuring embodiment 1-5 respectively, add water 10mL, shakes up, with it is water saturated just
Butanol shaking is extracted 4 times, and each 20mL merges extract solution, is evaporated, residue adds methanol 10mL to make dissolving and goes to 100mL measuring bottles
In, scale is added water to, is shaken up.Precision measures 1mL, puts in 25mL measuring bottles, the method under sighting target directrix curve preparation, " adds water certainly
Risen to 6mL " and determine absorbance in accordance with the law, the amount of rutin from need testing solution is read on standard curve is calculated, produced.Specific inspection
Survey the results are shown in Table 3.
The Determination of Total Flavonoids result of table 3
| Polyoses content | |
| Embodiment 1 | 467μg/mL |
| Embodiment 2 | 468μg/mL |
| Embodiment 3 | 465μg/mL |
| Embodiment 4 | 467μg/mL |
| Embodiment 5 | 465μg/mL |
By table 1- tables 3, contain more polysaccharide, aldehydes matter and brass class material in extract solution, surface is carried
Take liquid that there is good anti-oxidation function, while illustrating that the content of anti-oxidation active substance in extract solution is high, total class is complete.Explanation
The extraction effect of the extraction process of anti-oxidation active substance provided in an embodiment of the present invention is good, can be by the anti-oxidant work in lotus rhizome
Property material is fully extracted.
Experimental example 4
The measure of DPPH free radicals
The preparation of reagent：A (0.1mmol/L DPPH solution), B (0.05mg/mL sample liquid), C (60% ethanol solution),
The 0.05mg/mL of embodiment 1- embodiments 5 sample liquid.
Some test tubes and in sequence number consecutively are taken, each group adds reagent according to shown in table 1, test tube is placed in into 25 respectively
30min is reacted in DEG C thermostat water bath, because DPPH is shown in certain it is noted that keeping away in the labile property of light, whole experiment process
A517 is surveyed after the completion of light, water-bath.It is compared by positive control of Vc.Its clearance rate calculation formula is as follows：
Experiment addition reagent in the DPPH radicals scavengings of table 4
| Group | A/mL | B/mL | C/mL |
| Blank group | 3 | 0 | 3 |
| Sample sets | 3 | 3 | 0 |
| Control group | 0 | 3 | 3 |
It the results are shown in Table 5：
| DPPH free radical scavenging activities (%) | |
| Embodiment 1 | 70 |
| Embodiment 2 | 73 |
| Embodiment 3 | 74 |
| Embodiment 4 | 75 |
| Embodiment 5 | 73 |
In summary, the extraction process of 1-5 of embodiment of the present invention anti-oxidation active substances is powerful using the homogeneous method of high speed
Machinery and fluid shear destroy the intact cell configuration of lotus rhizome, are extracted beneficial to anti-oxidation active substance in lotus rhizome.Adopt simultaneously
Lotus rhizome is pre-processed with freezing, crushing and defrosting, the extraction efficiency of anti-oxidation active substance is further improved, simultaneously
The species that perfect anti-oxidation active substance is extracted.And extracted by the way of heating, accelerate anti-oxidation active substance
The speed and content of dissolving in a solvent, improve the efficiency and speed of extraction.Extraction is obtained into anti-oxidation active substance
Particle is prepared as, the storage time of anti-oxidation active substance is extended, while easy to carry and transport.
Embodiments described above is a part of embodiment of the invention, rather than whole embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiment in the present invention, what those of ordinary skill in the art were obtained under the premise of creative work is not made
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. a kind of extraction process of anti-oxidation active substance, it is characterised in that comprise the following steps：Lotus rhizome slurry is even using high speed
Matter method is carried out homogeneous and extracted；Wherein, the rotating speed of the homogeneous method of high speed is 6000~14000r/min, the temperature of extraction for 50~
90℃。
2. the extraction process of anti-oxidation active substance according to claim 1, it is characterised in that the preparation of the lotus rhizome slurry
It is to crush to obtain the lotus rhizome slurry after lotus rhizome is cleaned.
3. the extraction process of anti-oxidation active substance according to claim 2, it is characterised in that after the lotus rhizome is cleaned
Crushing is to clean the lotus rhizome freezed, crushed and defrosting processing successively.
4. the extraction process of anti-oxidation active substance according to claim 3, it is characterised in that the temperature of freezing for-
30-- 18 DEG C, cooling time is 24-48h.
5. the extraction process of anti-oxidation active substance according to claim 1, it is characterised in that the lotus after will be homogeneous
Lotus root slurry leaches 1-4 hours in 50~90 DEG C of water bath with thermostatic control.
6. the extraction process of anti-oxidation active substance according to claim 5, it is characterised in that by the lotus after leaching
Lotus root slurry progress, which is centrifuged, obtains supernatant, and the rotating speed of centrifugation is 3500-4500r/min, and centrifugation time is 5-8 minutes.
7. the extraction process of anti-oxidation active substance according to claim 6, it is characterised in that carried out to the supernatant
Concentration, concentrates 3-5 times.
8. a kind of preparation technology of antioxidation activity particle, it is characterised in that comprise the following steps：Appointed according to claim 1-7
The extraction process of anti-oxidation active substance described in meaning one extracts anti-oxidation active substance；The anti-oxidation active substance is entered
Row spray drying and wet granulation.
9. the preparation technology of antioxidation activity particle according to claim 8, it is characterised in that carry out spray drying be by
Supernatant after concentration, which is passed through spray drying tower and is dried, obtains extract powder, and the EAT of the spray drying tower is 160
~180 DEG C, 75~85 DEG C of leaving air temp, peristaltic pump 200~300r/min of charging rate, compressed air pressure 0.2-0.5MPa.
10. the preparation technology of antioxidation activity particle according to claim 9, it is characterised in that wet granulation is by institute
It is 1 that extract powder, sucrose and dextrin, which are stated, according to mass ratio:2-3:After the mixing of 0.8-1.2 ratios, it is mixed into after being ground with ethanol
Row moistening obtains softwood.
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