CN105287671A - Application of ginseng pectin in resisting depressive behavior - Google Patents
Application of ginseng pectin in resisting depressive behavior Download PDFInfo
- Publication number
- CN105287671A CN105287671A CN201510724008.7A CN201510724008A CN105287671A CN 105287671 A CN105287671 A CN 105287671A CN 201510724008 A CN201510724008 A CN 201510724008A CN 105287671 A CN105287671 A CN 105287671A
- Authority
- CN
- China
- Prior art keywords
- ginseng
- behavior
- pectin
- wgpa
- medicine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to application of ginseng pectin in resisting depressive behavior, and belongs to the field of traditional Chinese medicine application. Ginseng pectin is given to a mouse for 12 days in force feeding manner, and the activity of resisting a depressive behavior is evaluated by an open-field locomotor activity test, an elevated plus maze test, a social interference test and a forced swimming test. Results indicate that by using the ginseng pectin, the interference time and frequency in a mouse social interference test can be increased, the invasion time and frequency of the mouse can be reduced, the time rate and frequency rate of an open-arm of the mouse entering an elevated plus maze can be increased, the standing time of the mouse forced swimming test can be reduced, and protein expression of hippocampus beta-catenin and brain-derived neurotrophic factor (BDNF) of the mouse can be increased along with changes of the behaviors, namely the ginseng pectin can be used for enhancing release of transmitters among synapses and connection among synapses by inducing the protein expression of hippocampus beta-catenin and BDNF so as to influence the neuron plasticity and improve neuron development and achieve the activity of resisting the depressive behavior.
Description
Technical field
The present invention relates to the application of a kind of ginseng pectin in the behavior of antidepressant sample, belong to application in TCM field.
Background technology
Radix Ginseng is since ancient times just with building body, life lengthening and famous, and it has resisting fatigue, antioxidation, Auto-regulator metabolism, regulates the effects such as central nervous system.The neuroprotective of Radix Ginseng and extract thereof is extensively confirmed, if Radix Ginseng is by Neuroprotective Mechanisms antagonism Parkinson's disease (VanKampen, J.M., Baranowski, D.B., Shaw, C.A., & Kay, D.G. (2014) .Panaxginsengisneuroprotectiveinanovelprogressivemodelof Parkinson'sdisease.ExperimentalGerontology, 50, 95-105.), in addition, its extract such as Ginsenoside Rg3 has also been proved to be able to the cytotoxicity of antagonism 24-hydroxyl-cholesterol induction, protection cortical neuron (Roh, Y.S., Kim, H.B., Kang, C.W., Kim, B.S, Nah, S.Y, & Kim, J.H. (2010) .NeuroprotectiveeffectsofginsenosideRg3against24-OH-chol esterol-inducedcytotoxicityincorticalneurons.
journalofGinsengResearch, 34, 246-253.).Ginseng pectin, as the active component of Radix Ginseng, has antitumor (Shin, H.J., Kim, Y.S., Kwak, Y.S., Song, Y.B., Kim, Y.S., & Park, J.D. (2004) .Enhancementofantitumoreffectsofpaclitaxel (taxol) incombinationwithredginsengacidicpolysaccharide (RGAP).
plantaMedica, 24, 1033-1038.), anti-oxidation stress (Luo, D.H., & Fang, B.S. (2008) .Structuralidentificationofginsengpolysaccharidesandtest ingoftheirantioxidantactivities.CarbohydratePolymers, 72, 376-381.) and suppress diabetes (Konno, C., Murakami, M., Oshima, Y., & Hikino, H. (1985) .IsolationandhypoglycemicactivityofpanaxansQ, R, S, TandU, the biological function such as glycansofPanaxginsengroots.JournalofEthnopharmacology14: 69-74.).
Inventor's experimental result in earlier stage has proved that ginseng pectin WGPA obviously can reverse the set time of mouse forced swimming test, there is antidepressant activity (Wang, J, Flaisher-Grinberg, S., Li, S.S., Liu, H.B., Sun, L., Zhou, Y.F., etal. (2010) .Antidepressant-likeeffectsoftheactiveacidicpolysacchari deportionofginsenginmice.JournalofEthnopharmacology, 132,65-69.), however its antidepressant active component and molecular mechanism not yet verify.The present invention have studied the antidepressant activity of ginseng pectin and the Molecular Biology Mechanism thereof.
Summary of the invention
Instant invention overcomes the vacancy to the application of ginseng pectin's antidepressant in prior art, the using value new for ginseng pectin contributes, and provides experiment basis to the industrialization development of Radix Ginseng extract.
The invention provides the application of a kind of ginseng pectin WGPA-A in the behavior of antidepressant sample.
The present invention also provides a kind of ginseng pectin the application in preparation antidepressant sample behavior medicine, and described antidepressant sample behavior medicine is the compound recipe form formed with other ingredients with similar effect for active drug composition or ginseng pectin's acidic components WGPA-A with ginseng pectin's acidic components WGPA-A.
Described antidepressant sample behavior is two-way affective disorders, social aggressive behavior or anxiety etc.
The present invention also provides the medicine of a kind of antidepressant sample behavior, and described pharmaceutical pack is containing ginseng pectin's acidic components WGPA-A.
First the present invention prepares ginseng pectin WGPA, is then separated the neutral component WGPA-N and the acidic components WGPA-A that obtain ginseng pectin.
The present invention gives BS mice various dose ginseng pectin WGPA-A in advance, tested by autonomic activities, Elevated plus-maze, social interference experiment and forced swim test evaluate the antidepressant activity of ginseng pectin, the protein expression of key regulator beta chain albumen (Beta-catenin) in Wnt signal path and Brain Derived Neurotrophic Factor (BDNF) is detected, to inquire into its mechanism of action by WesternBlotting.
Result display ginseng pectin significantly increase mice society interference experiment in interference time, number of times, reduce mice invasion the time, number of times; Add mice and enter the time rate and time digit rate that open wide arm in Elevated plus-maze; What reduce mouse forced swimming test maintains static the time, and the behavior is learned result and shows that ginseng pectin WGPA-A has antidepressant activity.Simultaneously, above-mentioned ethological change increases along with the protein expression of Hippocampus of Mice Beta-catenin and BDNF, its mechanism may be raised by the protein expression of inducing mouse Hippocampus Beta-catenin and BDNF, strengthen the release of mediator between synapse, first plasticity that affects the nerves and the neuronic growth of promotion, thus play its antidepressant activity.
The present invention demonstrates by series of experiments the activity that ginseng pectin's acidic components have anti-two-way affective disorders.Its biologic activity is mainly through raising the protein expression of BDNF, activation Wnt/ β-catenin signal path, promote that β-catenin synthesis increases, and then promote neuronic survival, form occurs, the function of synapse and increase neuronic plasticity, plays the effect of anti-two-way affective disorders.
Although behavioristics's presentation of rodentine two-way affective disorders is difficult to evaluate, but go checking mice to be proved to be a kind of effective means evaluating two-way affective disorders to the exploratory behaviour of novel environment by Behaviors survey and social interaction experiment, ginseng pectin's acidic components WGPA-A obviously can reduce invasion time rate, the invasion time digit rate of the experiment of mice social interaction, the time of remarkable increase mice social interaction experiment and number of times, namely ginseng pectin's acidic components significantly increase social interaction ability, decrease social aggressive behavior simultaneously.
Elevated plus-maze test mice enters anxiety-like behavior and the medicine pharmacological activity antianxity that time of unlimited arm and number of times can be used for evaluating mice, ginseng pectin's acidic components WGPA-A can significantly increase in mouse elevated plus-maze experiment (open wide arm/open wide arm+airtight arm) time rate and secondary digit rate.Mice enters time of unlimited arm and number of times significantly to be increased than the time and number of times that enter airtight arm and means Set back by drug mouse anxiety sample symptom.
In addition, ginseng pectin's acidic components WGPA-A can reduce the set time of mouse forced swimming test, increases its swimming time, and the acidic components of prompting ginseng pectin obviously can reverse the depressive symptom of two-way affective disorders.And above-mentioned behavioristics's performance does not change with the autonomic activities (no matter being the test of 10min or the test of 60min) of mice prologue behavior, illustrate that the impact of WGPA-A on above-mentioned Behaviors survey result is not because change mice behavior baseline generally, cause mice manic or depressed.
beneficial effect of the present invention:
At present, the medicine overwhelming majority of depression passes through chemosynthesis, prolonged application, many toxic and side effects are brought to body, therefore, an urgent demand is found has antidepressant function and the natural product had no side effect, and Radix Ginseng has been proved to be able to regulate hypothalmus-pituitary-adrenal axis and monoaminergic system and antagonism is cognitive and anxiety dysfunction, therefore, will have broad application prospects with natural product-Radix Ginseng extract antagonism depression.Ginseng pectin WGPA comprises two kinds of components, and research before has proved that WGPA has antidepressant activity, and the unknown specifically any active component work, the present invention confirms that the concrete active component of ginseng pectin is Radix Ginseng pectic acid component WGPA-A.
Tradition is thought, the active component of Radix Ginseng to the regulating action of central nervous system is ginsenoside, therefore, in Radix Ginseng leaching process, ginseng polysaccharide is often abandoned in a large number as residue, cause very large waste, the present invention demonstrates ginseng pectin's acidic components WGPA-A by serial experiment and has antidepressant activity.Its biologic activity is mainly through raising the protein expression of BDNF, and activation Wnt/Beta-catenin signal path, promotes that the synthesis of beta chain albumen increases, increase neuronic plasticity, play antidepressant effect.This is by making full use of for Radix Ginseng component, and Radix Ginseng is farthest developed provides important working foundation.
Accompanying drawing explanation
Fig. 1: ginseng pectin's acidic components WGPA-A is to the test result of mice at Elevated plus-maze; Figure A is [unlimited arm/(opening wide arm+airtight arm)] time rate result in mouse elevated plus-maze experiment; Figure B be secondary digit rate result, and Post-hocLSDtest:* represents to have significant difference (p<0.0001) compared with saline control group.
Fig. 2: ginseng pectin's acidic components WGPA-A on mice social interaction interference time (A) and the impact disturbing number of times (B); * represent has significant difference (p<0.05) compared with saline control group.
Fig. 3: ginseng pectin's acidic components WGPA-A on the invasion time rate in mice social interaction experiment (10 minutes) and the impact of invading time digit rate; * represent has significant difference (p<0.05) compared with saline control group.
Fig. 4: ginseng pectin's acidic components WGPA-A is on the impact of the set time of mouse forced swimming test; * represent has significant difference (p<0.002) compared with saline control group.
Fig. 5: the WesternBlotting protein blot result of BDNF and Beta-catenin of mice after administration, A is the marking result of BDNF, and B is the protein blot result of Beta-catenin.
Fig. 6: the protein expression percentage result of BDNF and Beta-catenin of mice after administration, A is the protein expression percentage result of BDNF, and B is the protein expression percentage result of Beta-catenin; * the meaning (p<0.05) that to represent compared with saline control group that there was a significant difference.
Detailed description of the invention
embodiment 1: the preparation of ginseng pectin
Preparation ginseng pectin WGPA:
Extraction and isolation process is as follows: Radix Ginseng (commercially available) boils through hot water to be carried, and macroporous resin separates ginsenoside, and residue precipitate with ethanol obtains crude polysaccharides.Crude polysaccharides is refined through Sevag method deproteinization and precipitate with ethanol and is obtained total polysaccharides WGP(Watersolubleginsengpolysaccharides).WGP is divided into amyloid polysaccharide WGPN(Starch-likeglucans by DEAE-Cellulose ion exchange column) and ginseng pectin WGPA(Ginsengpectin).WGPA is dissolved in water again, adopts DEAE-Cellulose ion exchange column to be separated, carries out gradient elution, thus obtain neutral component WGPA-N and the acidic components WGPA-A of ginseng pectin with NaCl aqueous solution (0M, 0.5M).
embodiment 2: Behaviors survey
BlackSwiss mice purchased from Jiangsu University, 10-12 week age, be divided into three groups, often organize 10, matched group is normal saline group, administration volume 10mL/kg; Experimental group is 2 groups, ginseng pectin WGPA-A100mg/kg group and 200mg/kg group.
(1) autonomic activities of beginning is tested
After administration the 8th day, mice was placed in the transparent automatic movable monitor of a 50cm × 25cm × 20cm (OptoM3, ColumbusInstruments, Columbus, OH).The every 10min of infrared observation records 1 time, continuously record mice autonomic activities 6 times, and 60min altogether, after having tested, mice is sent to return in rearging cage, and cleans monitor with the ethanol of 10%.
Prologue autonomic activities experimental result is in table 1, and no matter in front 10min or in 60min, WGPA-A does not affect the autonomic activities time of mice, and namely ginseng pectin does not affect the change of mice behavior baseline.
The affect result of table 1WGPA on mice autonomic activities result is used
represent
N.S=Nosignificance, does not have significant difference
(2) Elevated plus-maze test (EPM)
Elevated plus-maze is wooden, the labyrinth of the cross shape of the 60cm that is above the ground level, and is made up of four arm, wherein comprises two airtight arms (24cm) and two unlimited arms (1cm).Every arm is all made up of jointly the region of 30cm × 5cm and the center of 5cm × 5cm.After administration the 9th day, first mice is placed on the center in labyrinth, by its behavioral activity of the camera record above labyrinth 5min, then according to shooting record manual count mice at time of unlimited arm and airtight arm and number of times, only have when being just recorded in the whole arm entry of mice extremity.Time in arm entry and number of times will be used for statistical analysis open wide arm time/(open wide arm time+airtight arm time); Open wide arm number of times/(opening wide arm number of times+airtight arm number of times).All sent after every mouse experiment terminates and returned its rearging cage, and cleaned maze device with 10% ethanol.Compared with normal saline group, WGPA-A100mg/kg and WGPA – A200mg/kg significantly increases the time rate ［ F (2 entering unlimited arm of mice, 27)=30.2, p<0.0001 ］ (see Figure 1A) and time digit rate ［ F (2,27)=53.1, p<0.0001 ］ (see Figure 1B).
Elevated plus-maze test mice enters anxiety-like behavior and the medicine pharmacological activity antianxity that time of unlimited arm and number of times can be used for evaluating mice, ginseng pectin's acidic components WGPA-A can significantly increase in mouse elevated plus-maze experiment (open wide arm/open wide arm+airtight arm) time rate and secondary digit rate.Mice enters time of unlimited arm and number of times significantly to be increased than the time and number of times that enter airtight arm and means Set back by drug mouse anxiety sample symptom.
(3) social interference experiment
After administration the 10th day, mice is from its rearging cage, be paired transfer has entered, 10min in unfamiliar test-cage, the mice of pairing should be same treatment group or normal saline group, the behavioral activity 10min of pairing mice is recorded continuously, then according to attack time and the social interference time of shooting record manual count every mice by the video camera above it.Attack time is defined as attempting stinging, actually sting, box, the behavior such as to play.Society is defined as the Body contact of another kind of form interference time, comprises smelling, licking or the contact of health.Do not carry out between mice contacting with each other and not being recorded.Invasion ratio be defined as invasion time/(invasion time+society interference time).After experiment terminates, mice is sent to return in rearging cage.
Result WGPA-A100mg/kg and WGPA-A200mg/kg all significantly affects the result of social interference experiment.Compared with normal saline group, WGPA-A significantly increases social interference time ［ see Fig. 2 A, F(2,27)=13.4, p<0.0001 ］ of mice and society's interference number of times ［ see Fig. 2 B, F(2,27)=6.91, p=0.004 ］.Meanwhile, WGPA-A significantly reduce the invasion time rate of mice and invasion time digit rate see Fig. 3, time rate: F(2,27)=3.65, p=0.04; Secondary digit rate F(2,27)=9.8, p<0.001].
Demonstrate invasion time rate, invasion time digit rate (intrusion behavior/non-intrusive behavior) that ginseng pectin's acidic components WGPA-A obviously can reduce the experiment of mice social interaction, the time of remarkable increase mice social interaction experiment and number of times, namely ginseng pectin's acidic components significantly increase social interaction ability, decrease social aggressive behavior simultaneously.
(4) forced swim test
The experimentation of forced swim test is by (PorsoltR.D., BertinA., JalfreM., etal (1978). " Behaviouraldespair " inratsandmice:straindifferencesandtheeffectsofimipramine .EurJPharmacol51 (3): 291-294.) carry out.BS mice after administration the 11st day, by putting into respectively, the plastic cylinder 25cm(filling water is high) × 10cm(diameter), cylinder fills 23 ~ 25 DEG C, the water that 10cm is high, mice is forced in 6min in plastic cylinder, when mice stops struggling in plastic cylinder, floating state (only having small action to surface to keep head) is kept to be considered to maintain static.Total testing time continues 6min, and front 2min is as the laundering period, and rear 4min records its swimming time by Enthovision8.5 auto Analysis and maintains static the time.Every mice has been swum, and all needs to change the water in cylinder.Result WGPA-A100mg/kg and 200mg/kg all significantly reduces set time [F2,27=8.64, the p<0.002] (see figure 4) of forced swim test.
Test proves, ginseng pectin's acidic components WGPA-A can reduce the set time of mouse forced swimming test, increases its swimming time, and the acidic components of prompting ginseng pectin obviously can reverse the depressive symptom of two-way affective disorders.
embodiment 3: Mechanism Study
The preparation of cerebral tissue: administration the 12nd day, mice 1h after administration is put to death by CO2 overdose of anesthesia, gets its Hippocampus and is placed in liquid nitrogen, be transferred in-80 DEG C of refrigerators.Homogenate: cerebral tissue sample adds the 50mMKPO4 buffer of 9 × volume, the Triton-X-100 and 1 of 0.5%pH7.2 × not containing in the protease inhibitor of EDTA, use the homogenate of Barnant homogenizer, brain homogenate thing is in 4 DEG C, 13, the centrifugal 15min of 000g, gets supernatant and is stored in-80 DEG C of refrigerators, and its protein concentration uses BCA protein assay kit to detect.
Beta chain albumen WesternBlotting analyzes: Beta-catenin applied sample amount is 1 μ g/ hole, in loading to 8% gel, SDS-PAGE electrophoresis (100V, 1h), isolated protein delivery is in pvdf membrane, 5% defatted milk powder closes 1h, rabbit source Anti-betacatenin polyclone primary antibodie hatches (1:5000, Abcam, Inc, Cambridge, ab32572) 2h, Anti-rabbitIgG bis-anti-(1:1000, BeyotimeBiotechnology, A0208) hatch 1h, Labworks image acquisition and analysis software detects.1h is hatched in Mus source Beta-tubulin primary antibodie (1:1000, BeyotimeBiotechnology, AT819), and Anti-mouseIgG bis-anti-(1:1000, BeyotimeBiotechnology, A0216) hatches 1h, and Labworks image acquisition and analysis software detects.Expressing quantity uses Bio-RadQualityOne analysis software to add up.Pattern detection result corrects (having run same check sample in each glue) by check sample, corrects finally by Beta-tubulin, ensures the consistent of applied sample amount.
Brain Derived Neurotrophic Factor WesternBlotting analyzes: BDNF applied sample amount is 5 μ g/ holes, in loading to 10% gel, and SDS-PAGE electrophoresis (100V, 1h), isolated protein delivery is in pvdf membrane, and 5% defatted milk powder closes 1h, rabbit source Anti-BDNF polyclone primary antibodie hatches (1:1000, Abcam, Inc, Cambridge, ab6201) 2h, Anti-rabbitIgG bis-anti-(1:1000, BeyotimeBiotechnology, A0208) hatch 1h, Labworks image acquisition and analysis software detects.1h is hatched in Mus source Beta-tubulin primary antibodie (1:1000, BeyotimeBiotechnology, AT819), and Anti-mouseIgG bis-anti-(1:1000, BeyotimeBiotechnology, A0216) hatches 1h, and Labworks image acquisition and analysis software detects.Expressing quantity uses Bio-RadQualityOne analysis software to add up.Pattern detection result corrects (having run same check sample in each glue) by check sample, corrects finally by Beta-tubulin, ensures the consistent of applied sample amount.
Fig. 5 is the WesternBlotting marking result of BDNF and Beta-catenin of mice after administration, and A is the marking result of BDNF, and B is the protein blot result of Beta-catenin; Fig. 6 is the protein expression percentage result of BDNF and Beta-catenin of mice after administration, and A is the protein expression percentage result of BDNF, and B is the protein expression percentage result of Beta-catenin; * the meaning (p<0.05) that to represent compared with saline control group that there was a significant difference.Result shows, and WGPA-A significantly increases the protein expression percentage ratio of BDNF and Beta-catenin in hippocampus of mice district.
Claims (7)
1. the application of ginseng pectin in preparation antidepressant sample behavior medicine.
2. the application of ginseng pectin according to claim 1 in preparation antidepressant sample behavior medicine, it is characterized in that, described ginseng pectin's active component is Radix Ginseng pectic acid component WGPA-A.
3. the application of ginseng pectin according to claim 2 in preparation antidepressant sample behavior medicine, it is characterized in that, the protein expression of described ginseng pectin's acidic components WGPA-A energy inducing mouse Hippocampus Beta-catenin and BDNF raises, strengthen the release of mediator between synapse, the plasticity of the unit that affects the nerves and the neuronic growth of promotion.
4. the application of the ginseng pectin according to claim 1-3 any one claim in preparation antidepressant sample behavior medicine, it is characterized in that, the behavior of described antidepressant sample is two-way affective disorders, social aggressive behavior or anxiety etc.
5. a medicine for antidepressant sample behavior, is characterized in that, described pharmaceutical pack is containing ginseng pectin's acidic components WGPA-A.
6. the medicine of a kind of antidepressant sample according to claim 5 behavior, is characterized in that, described medicine is for active drug composition with ginseng pectin's acidic components WGPA-A single component.
7. the medicine of a kind of antidepressant sample according to claim 6 behavior, is characterized in that, described medicine is the compound recipe form that Radix Ginseng pectic acid component WGPA-A and other ingredients with similar effect form.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510724008.7A CN105287671A (en) | 2015-10-30 | 2015-10-30 | Application of ginseng pectin in resisting depressive behavior |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510724008.7A CN105287671A (en) | 2015-10-30 | 2015-10-30 | Application of ginseng pectin in resisting depressive behavior |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105287671A true CN105287671A (en) | 2016-02-03 |
Family
ID=55185958
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510724008.7A Withdrawn CN105287671A (en) | 2015-10-30 | 2015-10-30 | Application of ginseng pectin in resisting depressive behavior |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105287671A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108741036A (en) * | 2018-06-19 | 2018-11-06 | 中国农业科学院特产研究所 | It is a kind of that there is the ginseng pectin's composition for adjusting intestinal flora function and its application |
| CN109258573A (en) * | 2018-11-21 | 2019-01-25 | 梧州学院 | Natural enemy mewing audio induces construction method and the application of Animal Models of Anxiety |
| CN109730322A (en) * | 2018-12-10 | 2019-05-10 | 华南农业大学 | Application of the panaxan in terms of promoting feeding and alleviating anxiety behavior |
| WO2022036121A1 (en) * | 2020-08-12 | 2022-02-17 | Pharmavite, Llc | Prebiotic composition of pectin, beta-glucan, xylooligosaccharide and/or ashwagandha and a method of improving mood |
| CN115068498A (en) * | 2022-07-06 | 2022-09-20 | 东北师范大学 | Application of AG type pectin in prolonging life and resisting aging |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104644652A (en) * | 2013-11-22 | 2015-05-27 | 富力 | Application of 20(R)-ginsenoside Rg3 in preparation of drug for treating depression and the drug |
-
2015
- 2015-10-30 CN CN201510724008.7A patent/CN105287671A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104644652A (en) * | 2013-11-22 | 2015-05-27 | 富力 | Application of 20(R)-ginsenoside Rg3 in preparation of drug for treating depression and the drug |
Non-Patent Citations (2)
| Title |
|---|
| JIA WANG ET AL: "Antidepressant-like effects of the active acidic polysaccharide portion of ginseng in mice", 《JOURNAL OF ETHNOPHARMACOLOGY》 * |
| 王佳: "人参多糖抗疲劳和抗抑郁作用及其机制的研究", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108741036A (en) * | 2018-06-19 | 2018-11-06 | 中国农业科学院特产研究所 | It is a kind of that there is the ginseng pectin's composition for adjusting intestinal flora function and its application |
| CN109258573A (en) * | 2018-11-21 | 2019-01-25 | 梧州学院 | Natural enemy mewing audio induces construction method and the application of Animal Models of Anxiety |
| CN109730322A (en) * | 2018-12-10 | 2019-05-10 | 华南农业大学 | Application of the panaxan in terms of promoting feeding and alleviating anxiety behavior |
| WO2022036121A1 (en) * | 2020-08-12 | 2022-02-17 | Pharmavite, Llc | Prebiotic composition of pectin, beta-glucan, xylooligosaccharide and/or ashwagandha and a method of improving mood |
| CN115068498A (en) * | 2022-07-06 | 2022-09-20 | 东北师范大学 | Application of AG type pectin in prolonging life and resisting aging |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105287671A (en) | Application of ginseng pectin in resisting depressive behavior | |
| Kim et al. | Therapeutic potential of mesenchymal stem cells (MSCs) and MSC-derived extracellular vesicles for the treatment of spinal cord injury | |
| Lim et al. | Inhibitory effect of quercetin on propionibacterium acnes-induced skin inflammation | |
| Chakravarthi et al. | Effect of Glycyrrhiza glabra root extract on learning and memory in wistar albino rats | |
| Ozer et al. | Anti-inflammatory effect of a Nuphar lutea partially purified leaf extract in murine models of septic shock | |
| Aja et al. | Evaluation of anti-diabetic effect and liver enzymes activity of ethanol extract of Pterocarpus santalinoides in alloxan induced diabetic albino rats | |
| Wang et al. | Hypoglycemic Effect of Ethanol and Ethyl Acetate Extract of Phellinus baumii Fruiting Body in Streptozotocin‐Induced Diabetic Mice | |
| CN103622992B (en) | The purposes of hydrogen sulfide and its donor sodium hydrosulfide in treatment diabetes medicament is prepared | |
| Nhung et al. | Analgesic and antipyretic activities of ethanol extract of Gardenia jasminoides Ellis fruits in mice | |
| CN103860611A (en) | Crape myrtle extract as well as extraction method and application thereof | |
| CN101152208A (en) | Application of brown seaweed polyoses sulfate in preparing medicament for treating senile dementia | |
| CN102293785A (en) | Method for extracting secondary metabolites of insects or arthropods | |
| CN105327338A (en) | Medicine for treating ulcerative colitis and preparation method of medicine | |
| CN103622955A (en) | Application of ellagic acid to preparation of drugs for treating and preventing fungal infection of human body | |
| Sundaram et al. | Preliminary study to evaluate analgesic and behavioural effects of Lycopodium clavatum in experimental animals | |
| Alallan et al. | Anti-arthritic effects of Anchusa strigosa extracts on complete Freund’s adjuvant-induced arthritis in rats | |
| Han et al. | Preventive effect of small molecular fraction of Irpex lacteus (Agaricomycetes) fruiting body against chronic nephritis in mice and identification of active compounds | |
| CN103860572B (en) | The application of baicalin in the poisoning medicine of preparation treatment Ricin | |
| Erndt-Marino et al. | Membrane potential controls macrophage activation | |
| CN103070956B (en) | Biological adhesion colon positioning micro-chip for treating ulcerative colitis | |
| Freitas et al. | Involvement of glutamate and cytokine pathways on antinociceptive effect of Pfaffia glomerata in mice | |
| Modaresi et al. | Comparison of Antinociceptive Effect of Viola Odorata with Carbamazepine in Animal Seizure Model | |
| Habbu et al. | Antidiabetic potential of endophytic bacterial fraction of Murraya koenigii (L.) Spreng. in rats | |
| DE102014005975B3 (en) | Detection of cell-implemented genetic information pre-programmed or de novo programmed, natural or synthetic replicable cells or minimal cells that respond to a product mixture for the purpose of a screening process | |
| CN103622984A (en) | Application of baicalin in preparation of medicine for treating acute hemolytic uremic syndrome |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20160203 |